|
|
|
| UFDC Home | myUFDC Home | Help |
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Full Text | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
PAGE 15 Oxidative Stress Hypoxia in vivo in vitro in vivo PAGE 16 .6, 7 .11 PAGE 17 Hyperoxia PAGE 18 Effect of Oxidative Stress on Endothelial Cell Metabolism PAGE 19 de novo de novo PAGE 22 Purine and Pyrimid ine Cycle in Oxidative Stress de novo de novo PAGE 24 Carbon Fiber Ultrami croelectrodes in vitro in vivo PAGE 27 Fast Scan Cyclic Volt ammetry (FSV) in vivo PAGE 28 High P erformance L iquid C hromatography (HPLC) PAGE 29 Study Overview PAGE 31 . PAGE 35 600400200 0 -200 0 2 4 6 current/ nApotential/ mV PAGE 36 injector PAGE 37 Reagents and Solutions PAGE 38 Cyclic Voltammetry PAGE 39 Fast Scan Cyclic Voltammetry (FSV) Instrumental Setup PAGE 41 Electrodes PAGE 42 SEM Imaging Concentration Determination HPLC -UV Measurements PAGE 43 Enzyme Assays PAGE 44 Cells PAGE 45 Statistical Analysis PAGE 48 600400200 0 -200 0 1 2 3 4 5 PAGE 49 1.51.00.50.0-0.5-1.0 -8 -6 -4 -2 0 2 4 6 PAGE 50 Introduction PAGE 52 R esults and Discussion Nanostructured Carbon Fiber Sensor E lectrode (N -CFS ) Background Current in Physiological B uffers PAGE 55 Voltammetry of F erricyanide at the Carbon Fiber Sensor B efore and After Surface Fabrication PAGE 56 Voltammetry o f Uric Acid (U A) i n Physiological Media PAGE 57 N-CFS Fabrication for Use in Physiological Media PAGE 61 Conclusions PAGE 70 Introduction PAGE 72 R esults and Discussion Background Current and Surface Structure of the Carbon Fiber Sensor PAGE 75 Voltammetry of Ferricyanide Voltammetry of Xanthine PAGE 76 Sensitivity in Fast Scan Voltammetry of Xanthine PAGE 78 Xanthine and Uric Acid in Xanthinuric and Normal U rine PAGE 79 Direct Determinations of Metabolite Concentrations in Urine PAGE 80 Conclusions PAGE 88 Introduction de novo PAGE 89 de novo .2931, 153 PAGE 92 inal metabolites of the purine and pyrimidine cycle released in the cell supernatant were expected to change after exposure to stress.12, 27 Isocrati c elution with HPLC -UV was used, since it and allows good separation of purines, pyrimidines and their oxidation products .100 Results and Discussion Measurements of Metabolites in Cell Supernatant PAGE 93 In this work with a low ionic strength 20 mM buffer, pH 5.1. This allowed separation of neutral forms of structurally similar purine and pyrimidine metabolites Hy (pKa 8.8),169 XA (7.44),151 UA (5.8),170 Ur (9.5)171 and Ade (3.6)172 from their oxidation products. Longer retention times and higher capacity factors in isocratic elution favor separation of structurally similar metabolites such as 2,8 -D HA and UA, and Hy and XA. Some variations in retention times were observed, which may be due to changes in the pH of cell supernatants. Consequently, standards and enzyme assays were used to confirm metabolite identity. Ado and Ino were n ot detected in cell supernatants B reakdown of Ado to Hy is very rapid in endothelial cells due in part to the high activity of purine nucleoside phosphorylase.173 This suggests that ATP breakdown products are re leased from cells while some may be formed by the degradation of exogenous ATP Ado may be transported back into the cell to form Hy, XA and UA, which are then released .167 In vivo PAGE 94 Metabolite Identification in Cell Supernatant by HPLC -UV PAGE 96 Oxidative Stress PAGE 97 Effect of Hyperoxia on Adaptation of PAECs PAGE 98 in vivo H ypoxia at AECs PAGE 99 Observations of the Effects of Oxidative Stress PAGE 102 de novo in vivo in vivo PAGE 104 de novo de novo PAGE 105 - de novo PAGE 106 PAGE 107 Conclusions PAGE 117 I ntroduction PAGE 118 In vivo in vivo PAGE 119 R esults and Discussion FSV of 2,8-DHA in pH 7.4 P hosphate Buffer at N -CFS PAGE 121 FSV of 2,8-DHA at N-CFS in Physiological Buffers PAGE 123 Measurement of 2,8 -DHA in PAECs and AECs Supernatant in vivo PAGE 126 Conclusions PAGE 138 Free Radic. Biol. Med 30 Am. J. Physiol .Cell Physiol 292 Plant Physiol., 141 Am. J. Physiol -Cell Physiol., 282 J. Clin. Invest., 82 Circ. Res., 86 Radiat. Res., 143 Circ. Res., 97 Anal. Biochem., 238 Am J. Physiol .Lung Cell Molec Physiol 270 J. Neurochem., 51 Am. J. Respir. Cell Mol. Biol., 6 Mol. Immunol., 38 Life Sci., 59 J. Biol. Chem., 256 Crit. Care Med., 32 Chest, 88 Ann. Neurol., 57 PAGE 139 Neuroimaging Clin. N. Am., 15 J. Biol. Chem., 279 Physiol. Genomics, 30 Physiol. Genomics, 30 Biochem. J., 390 J. Clin. Invest., 95 Am. J. Physiol., 258 J. Cell Biol., 109 Am. J. Physiol. -Cell Physiol., 276 J. Bacteriol., 82 J. Neurochem., 46 Brain and Nerve (Tokyo), 41 Neurosurgery, 25 Am. J. Physiol., 266 Am. J. Respir. Crit. Care Med., 158 Biochem. J., 266 PAGE 140 Pharmacol. Ther., 84 Hoppe -Seylers Zeitschrift Fur Physiologische Chemie, 355 Circulation, 107 Am. J. Physiol. -Cell Physiol., 289 Am. J. Physiol. -Lung Cell. Mol. Physiol., 270 Am. J. Physiol. -Lung Cell. Mol. Physiol., 269 J. Cardiovasc. Pharmacol. Ther., 13 J. Biol. Chem., 273 J. B iol. Chem., 273 J. Hypertens., 18 Antioxid. Redox Signal., 5 J. Chromatogr. B, 877 Clin. Chem., 45 Arch. Biochem. Biophys., 376 Proc Natl Acad Sci U. S A. 78 Physio l. Rev., 87 J. Neurochem., 69 PAGE 141 J. Biol. Chem., 279 Pflugers Arch., 452 Mol. Cell. Biochem., 321 Circ. Res., 47 Jpn. J. Cancer Res., 84 J. Nutr., 126 IUBMB Life, 58 Appl. Environ. Microbiol., 61 J. Bacteriol., 188 Analyst, 128 Analyst, 133 Chem. Anal., 44 Anal. Biochem., 129 Pediatr. Nephrol., 22 Aktuelle Urol., 35 Clin. Chim. Acta, 335 J. Clin. Invest., 79 Clin. Biochem., 40 Clin. Chim Acta 160 PAGE 142 J. Braz. Chem. Soc., 11 J. Pharm. Biomed. Anal., 19 Anal. Chem., 72 Anal. Chi m. Acta, 349 Analyst, 132 Brain Res., 381 J. Phys. Chem., 88 Analyst, 128 J. Neurosci. Methods, 95 Science, 240 Anal. Chem., 66 48 Anal. Chem., 66 Chem. Eng. J., 116 Carbon Fibers Carbon: Electrochemical and Physical Properties In Laboratory Techniques in Electroanalytical Chemistry In Electroanalytical Chemistry Trends Neurosci., 12 PAGE 143 Anal. Chem., 68 Analyst, 128 Electroanalysis, 12 Analyst, 123 J. Neurosci. Methods, 10 Anal. Chem., 56 J Electroanal Chem, 272 Anal. Chim. Acta, 321 Zeitschrift Fur Elektrochemie, 57 Anal. Chem., 67 Anal. Chim. Acta, 202 Clin. Biochem., 38 Chem. Lett., J. Clin. Invest., 56 Am. J. Physiol., 257 J. Am. Chem. Soc., 104 Anal. Chem., 56 Anal. Chem., 67 J. Phys. Chem., 90 Anal. Chem., 57 PAGE 144 Ann. Anat. -Anat. Anz., 191 Placenta, 26 Am. J. Obstet. Gynecol 185 Free Radic. Biol. Med., 14 J. Leukoc. Biol., 61 Anal. Chem., 67 Anal. Chem., 71 Anal. Chem., 71 Anal. Chem., 57 Anal. Chem., 55 Electroanalysis, 19 Anal. Chem., 79 Electrochem. Commun., 6 J. Mass Spectrom 41 Circulation, 115 Nephron. Cli n. Pract., 105 Clin. J. Am. Soc. Nephrol., 2 Hypertension, 49 PAGE 145 J. Chromatogr. B, 781 Clin. Chem., 46 J. Chromatogr. B, 837 Arch. Med. Res., 38 Electrophoresis, 28 J. Liq. Chromatogr. Rel. Technol., 25 Chromatographia, 65 Anal. Biochem., 108 Anal. Chim. Acta, 89 Nanotechnol. 17 Methods in Molecular Biology Anal. Chem., 59 Anal. Chem., 65 Anal. Chem., 70 J. Electrochem. Soc., 147 Carbon, 39 Polym. Bull., 59 PAGE 146 J. Raman Spectrosc., 35 Electrochemical Methods, Fundamentals and Applications J. Chim. Phys 94 J. Chim Phys 93 Radiat. Res., 97 J. Electrochem. Soc., 119 Chem. Res. Toxicol., 16 Anal. Chem., 65 Acta Neurochir., 142 Arch. Biochem. Biophys., 348 Proc. Natl. Acad. Sci. U. S. A., 99 Mutat. Res 402 Circ. Res., 87 Am. J. Physiol. -Heart Circul. Physiol., 292 Proc. Natl. Acad. Sci. U. S. A., 103 Am J. Physiol 289 Am. J. Physiol. -Heart Circul. Physiol., 293 Pharmacol. Rev., 50 PAGE 147 Cancer Res., 54 Pathobiology, 58 J. Chromatogr. B, 707 J. Chromatogr. B, 704 Gen. Pharmacol., 27 Basic Res. Cardiol., 92 Clin. Chem., 29 QJM Mon. J. Assoc. Physicians, 93 Cancer Res., 16 Chromatographia, 47 Cardiovasc. Res., 28 Am. J. Physiol., 263 In Vitro Mol. Toxicol. -J. Basic Appl. Res., 14 Clinica Chimica Acta, 245 FASEB J., 23 J. Exp. Med., 198 Trends Pharmacol. Sci., 10 PAGE 148 Am. J. Physiol., 262 Am. J. Physiol. -Heart Circul. Physiol., 277 Am. J. Pathol., 145 J. Appl. Physiol., 59 Am. J. Respir. Cell Mol. Biol., 28 Biochem. -Moscow, 69 Biochem. Biophys. Res. Commun., 113 Am. J. Respir. Cell Mol. Biol., 7 Brain Res., 1008 Proc. Natl. Acad. Sci. U. S. A., 95 Proc. Natl. Acad. Sci. U. S. A., 89 Biochem. J., 249 Biochem. Biophys. Res. Commun., 122 Annu. Rev. Biochem., 30 Proc. Natl. Acad. Sci. U. S. A., 87 Free Radic. Biol. Med., 11 PAGE 149 Phytochemistry, 25 Topics and perspectives in adenosine research. Proc. Natl. Acad. Sci. U. S. A., 93 Int. J. Cardiol., 100 J. Cell. Mol. Med., 12 News. Physiol Sci 16 Am. J. Physiol. Cell Physiol., 42 Arch. Biochem. Biophys., 273 J. Appl. Physiol., 65 J. Cell. Physiol., 162 J. Biol. Chem. 283 Biochem. Biophys. Res. Commun., 251 Am. J. Physiol. -Cell Physiol., 295 Biochem. J., 255 Metab. -Clin. Exp., 40 Arch. Biochem. Biophys., 70 J. Chem. Soc PAGE 150 Bioelectrochem 70 Indian J. Chem. Sect A -Inorg. Phys. Theor. Anal. Chem., 32 Aust. N. Z. J. Med., 5 Journal of Chromatography A, 894 J. Chromatogr. B -Biomed. Appl., 672 Clin. Chim. Acta, 181 J Electroanal Chem, 557 J Electroanal Chem, 521 Free Radic. Biol. Med., 40 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
