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- Permanent Link:
- http://ufdc.ufl.edu/UFE0022191/00001
Material Information
- Title:
- Aluminum Water Treatment Residual Effects on Soil Phosphorus Retention and Forage Yield and Nutritive Value
- Creator:
- Driscoll, Julie Lauter
- Place of Publication:
- [Gainesville, Fla.]
- Publisher:
- University of Florida
- Publication Date:
- 2008
- Language:
- english
- Physical Description:
- 1 online resource (87 p.)
Thesis/Dissertation Information
- Degree:
- Master's ( M.S.)
- Degree Grantor:
- University of Florida
- Degree Disciplines:
- Soil and Water Science
- Committee Chair:
- Graetz, Donald A.
- Committee Members:
- Silveira, Maria L.
Rhue, Roy D.
- Graduation Date:
- 5/1/2008
Subjects
- Subjects / Keywords:
- Aluminum ( jstor )
Forage ( jstor ) Groundwater ( jstor ) pH ( jstor ) Phosphorus ( jstor ) Soil horizons ( jstor ) Soil science ( jstor ) Soils ( jstor ) Toxicity ( jstor ) Water treatment ( jstor ) Soil and Water Science -- Dissertations, Academic -- UF bahiagrass, phosphorus, residual, treatment, water City of Madison ( local )
- Genre:
- Electronic Thesis or Dissertation
bibliography ( marcgt ) theses ( marcgt ) Soil and Water Science thesis, M.S.
Notes
- Abstract:
- The health of aquatic systems is being threatened by the addition of phosphorus (P) which causes increased eutrophication. Many Florida soils have naturally low capacities to retain P, allowing for P loss to local waterways by leaching. In addition, P is often over-applied when animal manures are used as fertilizers, which further increases the chances of P loss. Water treatment residuals (WTRs) are by-products of the water purification process which contain high amounts of amorphous aluminum (Al) or iron, giving them the capacity to retain P. Land application of WTRs has been investigated mainly in greenhouse experiments to test the P sorbing capacities of WTRs and to evaluate potential Al toxicity to plants. The purpose of this project was to evaluate the effects of an Al-WTR on soil P retention, soil microbial population, groundwater soluble reactive P (SRP) concentrations and forage yield and nutritive value. Three WTR application rates (0, 1.5 and 3% dry weight by mass) and two application methods (soil incorporation and surface applied) were tested on a bahiagrass (Paspalum notatum) pasture in Ona, FL. Soils on the site had low P levels (A horizon = 2.1 mg Mehlich-1 P kg-1) so triple superphosphate was added at 224 kg ha-1 to simulate high P loadings. The addition of WTR showed a trend toward slightly higher pH. Water extractable P concentrations decreased as WTR application rate increased, suggesting less P will be lost to leaching. Mehlich-1 Al increased from 22 mg kg-1 to 390 mg kg-1 (3% WTR rate) in the A horizon, giving the soil a greater P retention capacity which was observed in the increasing soil P storage capacity (SPSC) values which occurred with increasing WTR rate as well. The SPSC values increased on plots with WTR even after heavy loads of P were applied and some remained high enough that additional P should be retained. Potential nitrification rates increased with higher WTR application rates, indicating no adverse effects occurred on soil microbial populations because of WTR application. Groundwater SRP concentrations decreased with the addition of WTR by 78% in the surface applied treatments. There were no differences in SRP concentrations among the incorporated treatments which was likely a temporary result of the tilling process. Forage yield, crude protein and neutral detergent fiber were not affected by WTR application. Tissue Al did not increase and tissue calcium and magnesium uptake was not restricted. Tissue P decreased as WTR application rate increased. Tissue P levels were higher in the incorporated treatments than the surface applied treatments. However, tissue levels did not fall below the bahiagrass limiting value (0.15%). Based on this study, the use of WTRs is recommended as an amendment to soils with low P retention capacities that have been heavily impacted with P or will receive high amounts of P (e.g., a dairy sprayfield). ( en )
- General Note:
- In the series University of Florida Digital Collections.
- General Note:
- Includes vita.
- Bibliography:
- Includes bibliographical references.
- Source of Description:
- Description based on online resource; title from PDF title page.
- Source of Description:
- This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
- Thesis:
- Thesis (M.S.)--University of Florida, 2008.
- Local:
- Adviser: Graetz, Donald A.
- Statement of Responsibility:
- by Julie Lauter Driscoll
Record Information
- Source Institution:
- University of Florida
- Holding Location:
- University of Florida
- Rights Management:
- Copyright Julie Lauter Driscoll. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
- Embargo Date:
- 7/11/2008
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- LD1780 2008 ( lcc )
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concentrations were below the detection limit. Most of this P is bound by the extensive amount
of Al. Desorption of P from an Al-WTR was reported to be less than 1% (Makris et al., 2004).
Table 4-2. General characteristics of the Manatee County Al-WTR.
Moisture pH* Mehlich-1 Mehlich-1 Mehlich-1 Total Water
Content Al Fe P P** Extractable P
mgk-1
----------------------------------mg kg ---------------------
30.3% 6.8 1007 17 2 1300 BDL***
Soil:Solution ratio of 1:2
*Total P determined by ignition method (Anderson 1976)
*** Detection limit was 0.02 mg L-1
Soil pH
Soil pH eight months after WTR application was affected by application method for the
A (0-8 cm) and A (8-21 cm) horizons and nearly affected by WTR application rate in the A (0-8
cm) horizon (Table 4-3). There were no effects of WTR application method or rate in the E and
B horizons. In the A (0-8 cm) and A (8-21 cm) horizons differences resulted from application
method in which the incorporated treatments had a higher pH than the surface applied treatments
(Table 4-4).
Table 4-3. The p-values for the pH data using ANOVA and general mean contrast (single degree
of freedom).
Soil Horizon Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-'
A (0-8 cm) 0.009 0.0564 0.9094 0.0126 0.2683
A (8-21 cm) 0.0083 0.1034 0.6941 0.0403 0.6156
E 0.9536 0.6706 0.5785 0.7623 0.6168
B 0.9805 0.1316 0.3168 0.5134 0.0456
t Values italicized are significant at a = 0.05.
Table 4-4. Soil pH averages for incorporated and surface applied treatments 8 months after WTR
application.
Application Method A (0-8 cm) A (8-21 cm) E B
Incorporated 4.89 a 4.73 a 4.89 a 5.98 a
Surface Applied 4.71 b 4.43 b 4.93 a 6.18 a
t Treatments with similar letters show no significant difference for that column by Waller-
Duncan (p<0.05).
Table 4-10. The p-values for the forage yield data using ANOVA and general mean contrast
(single degree of freedom).
Month Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
January 0.9283 0.3292 0.4687 0.1426 0.9874
May <.0001 0.2742 0.4541 0.136 0.5674
June <.0001 0.1417 0.9699 0.077 0.3685
July 0.089 0.6099 0.6195 0.3596 0.7245
August 0.03 0.0799 0.0578 0.1147 0.0936
September 0.9674 0.3422 0.0845 0.3745 0.2444
t Values italicized are significant at a = 0.05.
ft January data was collected before any amendments were applied. May through September
data was collected after WTR and TSP application.
Treatment effects were evaluated on five harvests taken during May through September,
2007. The incorporation process caused yield reduction for the first two harvests (May and
June) because the grass was not completely reestablished from tilling (Table 4-11). In the
subsequent harvests, there were no yield differences observed except for August when yield was
greater in the incorporated than in the surface applied treatments. Differences did not occur in
the previous and following months so the August forage yield differences may be an anomaly.
However, it may take additional yield measurements in the following year to confirm this.
Table 4-11. Forage yield averages for incorporated and surface applied treatments.
Application Method January May June July August September
---------------------------------- Tons ha ----------------------
Incorporated 5.77 a 0.05 b 0.45 b 2.62 a 4.27 a 2.25 a
Surface Applied 5.74 a 0.41 a 1.44 a 2.91 a 3.90 b 2.25 a
f Treatments with similar letters show no difference for that column by Waller-Duncan (p<0.05).
In this field study, WTR application rates up to 3% dry weight by mass did not indicate
any trends toward negatively impacting bahiagrass yield. Oladeji et al. (2006) evaluated
bahiagrass yields in a glasshouse pot experiment. An Al-WTR was applied on a dry weight basis
at rates of 0, 1 and 2.5% dry weight by mass to a P deficient soil fertilized at N and P based rates.
Bahiagrass yields were reduced in the first harvest likely due to germination difficulties. In the
second crop, yields were not affected at any WTR application rate. In a study using stargrass
values suggesting the soils could retain additional P. The A and E horizons had low SPSC
values close to zero, or equilibrium, implying the soils could easily become P sources. Even
though these soils had low initial P concentrations, they also contained very little Al and Fe to
give them any significant P retention capacity.
The SPSC was affected by the addition of P and WTR. Application method affected
SPSC only in the A (0-8 cm) horizon while rate affected SPSC in all horizons except the B
horizon (Table 4-7). Both of the control treatments became significantly negative as a result of
adding P to a soil which had an initially low SPSC (Figs. 4-7 and 4-8). These soils have lost the
ability to retain P and prevent leaching. SPSC values increased as WTR application rates
increased at every horizon except the B horizon where P and Al levels were unaffected.
Table 4-7. The p-values for the SPSC data using ANOVA and general mean contrast (single
degree of freedom).
Soil Horizon Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
A (0-8 cm) 0.0004 <.0001 0.019 <.0001 0.0006
A (8-21 cm) 0.1036 0.0012 0.1043 0.0003 0.5023
E 0.2506 0.018 0.7193 0.0065 0.4346
B 0.4223 0.7748 0.6761 0.4874 0.9717
t Values italicized are significant at a = 0.05.
In the A horizon (0-8 cm), the incorporated treatments with WTR have greater SPSC
values than the original soils suggesting that the applied WTR increased the soil's ability to
retain P even after a large amount of P was added. However, Figure 4-8 shows that the surface
applied treatments with WTR were only slightly positive at the 70 Mg ha-1 application rate and
dropped below zero at the 35 Mg ha-1 application rate.
The E horizon soils treated with WTR continued to have SPSC values near zero similar
to the pre-amendment soils. While the WTR did not increase the soil's capacity to retain P at
ALUMINUM WATER TREATMENT RESIDUAL EFFECTS ON SOIL PHOSPHORUS
RETENTION AND FORAGE YIELD AND NUTRITIVE VALUE
By
JULIE LATER DRISCOLL
A THESIS PRESENTED TO THE GRADUATE SCHOOL
OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT
OF THE REQUIREMENTS FOR THE DEGREE OF
MASTER OF SCIENCE
UNIVERSITY OF FLORIDA
2008
Water extractable P concentrations are shown by horizon for the incorporated and surface
applied treatments in Figs. 4-1 and 4-2, respectively. Recall that 14 weeks after WTR was
applied to the appropriate treatments, a blanket application of P as triple superphosphate (TSP)
was surface applied at a rate of 224 kg P ha-1 to all plots including the controls. Concentrations
of WEP increased in the controls compared to the pretreatment concentrations due to this
application of P. Thus, data are also presented in these figures for WEP concentrations prior to
the application of P.
Higher Al concentrations suggest a greater potential of the soil to retain P. It also results
in a greater need to be aware of possible Al toxicity to plants. These potential effects will be
discussed in the forage section of this chapter.
Soil Phosphorus Storage Capacity
The soil P storage capacity (SPSC) was estimated from the following calculation
determined by Nair and Harris (2004):
SPSC (mg P kg-1) = (0.15 PSR) (Al + Fe)x 31
where PSR = (P) / (Al + Fe) and all elements are expressed as mmoles. The P, Al and Fe values
were Mehlich-1 extractable concentrations. The 0.15 value is the threshold for Florida soils
suggested by Nair and Harris (2004) and 31 is the atomic mass of P. When SPSC values are
greater than 0 the soil is acting as a P sink and when SPSC values are less than 0 the soil is acting
as a P source. The true SPSC calculation uses oxalate extractable concentrations instead of
Mehlich-1 concentrations. Therefore the SPSC values shown here do not give exact values but
are instead approximations in which relative changes can still be analyzed. There have been
other instances when Mehlich-1 values for P, Al and Fe were used to calculate SPSC values
(Nair and Harris, 2004).
The SPSC factor is unique in that it takes into account not only how much P the soil
contains from past loadings but also how much P retention capacity remains in the soil. Soil test
P (STP) concentrations are often used to determine environmental risks due to P, but a low STP
does not necessarily indicate that the soil has the capacity to retain added P (Nair and Harris,
2004).
The average SPSC values prior to application of any treatment for the A (0-8 cm), A (8-
21 cm), E and B horizons were 2, 4, 3 and 44 mg kg-1 respectively. The B horizon showed a
higher SPSC value due to higher concentrations of Fe and Al. All horizons showed positive
which should reduce P movement through the soil. Long term studies are needed to establish the
continual benefits ofWTRs on soil P retention. The soil microbial population was not adversely
affected as nitrification potentials increased as WTR application rate increased. The analysis of
soluble reactive P (SRP) in groundwater showed a decrease in the surface applied treatments
with WTR application but no changes among the incorporated treatments. Tillage seems to have
affected SRP concentrations but future sampling may show results without interference from the
incorporating process. Regular groundwater sampling in the future may also help to validate
current results.
Forage yield, NDF and crude protein were not affected by WTR application rate for the
duration of the study. Tissue analysis did not show additional uptake of Al and the uptake of Ca
and Mg was not restricted, suggesting Al has not interfered with cation uptake by roots. Tissue P
declined with added WTR but concentrations appear to have stabilized over the five harvests and
did not drop below the critical level for bahiagrass. Again, future analysis is needed to assure
tissue P does not drop any further. One of the issues associated with Al toxicity is the restriction
of root growth. This study did not attempt to measure root biomass because this would have
required complete removal of the bahiagrass on the study site. The field plots were preserved so
future research can be continued. Decreased root growth can result in less nutrient and water
uptake, affecting growth and yield. This situation has the potential to develop with time so
future sampling is needed to assure toxicity effects do not present themselves.
Based on this study, the use of WTRs is recommended as an amendment to soils with low
P retention capacity that have been heavily impacted with P or will receive high amounts of P,
e.g., a dairy sprayfield. The WTRs have shown to lower WEP concentrations below those of
untreated soil. The SPSC values have also shown an increase, even with high loads of applied P,
Table 4-1. General characteristics in soil samples taken prior to amendment application (August
2006) averaged for all plots.
Soil Horizon Soil pH Water Extractable P Mehlich-1 Mehlich-1 Mehlich-1 Al:Fe Ratio
P Al Fe
-----------------------------------mg kg--------------
A (0-8 cm) 4.59 9.0 + 3.8 2.1 + 1.2 22 9.9 4.6
A (8-21 cm) 4.42 3.0 + 1.6 1.4 2.7 35 14.7 5.0
E 4.36 0.8 + 0.8 0.7 + 1.9 20 9.0 4.5
Bh 5.62 1.3 + 1.4 29.1 + 16.7 334 71.7 9.7
Bw 5.40 1.9 3.3 36.1 16.6 287 54.1 11.0
Bt 6.37 2.0 + 0.9 38.4 19.5 131 25.4 10.7
Mehlich-1 Al and Fe concentrations were approximately 10 and 4 fold greater,
respectively, in the B horizons than in the A and E horizons (Table 4-1). This accumulation of
metals is a result of spodic horizon formation when metals and carbon are redistributed from one
zone to another (Franzmeier et al., 1965). The Al:Fe ratios in the A-E and B horizons were
approximately 5 and 11, respectively. It is typical for Fe concentrations to be lower than Al
concentrations in poorly drained Spodosols in Florida (Sodek et al., 1990). These spodic Al and
Fe values are similar to those found in a South Florida beef pasture by Nair and Graetz (2002).
Aluminum Water Treatment Residual Characteristics
The moisture content and pH of the Al-WTR were 30.3% and 6.8, respectively (Table 4-
2). Mehlich-1 Al and Fe concentrations were 1,007 and 17 mg kg-1, respectively. A recent study
using material from the Manatee County Water Treatment Plant found that nearly all the Al and
Fe was oxalate extractable (O'Connor, 2005). Oxalate extracts amorphous Al and Fe which are
highly reactive to P (McKeague et al., 1971). Another study also using material from the same
source at an earlier date found that approximately 80% of the Al was amorphous (Miyittah-
Kporgbe, 2004) and the total Al concentration was 89.1 g kg-1 (O'Connor et al., 2002). The
WTR had a substantial total P content (1,300 mg kg-1). Although this will result in a large
amount of P added to the soil, there is little chance of P release from the WTR since WEP
Tillage may cause pH to be higher than if the soil was left unmanaged. Godsey et al.
(2006) reported that over many years no-till plots of wheat, soybean and sorghum had a lower
average pH than conventionally tilled plots. No-tillage systems can increase the organic matter
(OM) of the soil. Shertz (1994) showed an example in which a farm in Illinois had an increase in
soil OM from 1.87% to 4.0% in fifteen years of no till. Higher soil OM levels can potentially
lead to an increase in soil acidity (Coleman and Thomas, 1967). Carbon dioxide is produced
when OM decomposes, which releases H+ when it reacts with water. Organic matter also
contains carboxylic and phenolic groups which act as weak acids (Havlin et al., 2005). This
acidifying action of soil OM has a minimal effect on the overall soil pH in mineral soils but can
still be a factor. No-till systems also have higher rates of water infiltration and thus leach cations
such as Ca+ which can be replaced by H+, causing higher rates of soil acidity over time (Powers
and McSorley, 2000).
Water Extractable Phosphorus
Water extractable P is the form of P most easily lost to leaching and subsurface transport.
Application method affected WEP concentrations in the A (8-21 cm) and E horizons while
application rate influenced WEP concentrations in the A (0-8 cm), A (8-21 cm) and E horizons
(Table 4-5). The interactions between WTR application method and rate were not significant.
Table 4-5. The p-values for the WEP data using ANOVA and general mean contrast (single
degree of freedom).
Soil Horizon Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
A (0-8 cm) 0.5962 <.0001 0.9011 <.0001 0.0341
A (8-21 cm) 0.0319 0.0004 0.223 0.0001 0.5329
E 0.0066 0.0004 0.9758 <.0001 0.5543
B 0.9731 0.447 0.5473 0.616 0.2504
t Values italicized are significant at a = 0.05.
in August and September. The RCREC received 7.4 cm of rain in May through August of 2007,
while the average amount of rain for the previous 4 years was 13.85 cm (FAWN).
Water samples were collected using a battery operated vacuum pump. Prior to sampling,
standing water in the wells was purged to assure that fresh water was being sampled. After
approximately one hour, the wells were recharged and 200 mL samples were collected into
Nalgene bottles. Samples were preserved with H2SO4 (1 drop per 20 mL sample). Samples were
placed on ice before refrigerated in the laboratory.
The groundwater table depth was measured using a pressure transducer to measure the
potential of water at varying depths (modeled from the design of Schumann and MunozCarpena,
2002). The transducer was calibrated by using a bucket of water at known water levels and
recording the voltage. A regression was then made from several values and an equation was
produced to convert voltage readings to water depth. A Hobo datalogger was set to record
voltage readings in the field every 15 minutes.
Analytical Methods
Soil
All soil samples were air dried before analysis. Water extractable P, Mehlich-1 P, Fe and
Al, pH and the soil P storage capacity were determined for all soil samples.
Water extractable P (WEP) was determined using a 1:10 soil to solution ratio (Kuo,
1996). Three grams of soil was weighed into 50mL centrifuge tubes and 30mL of distilled
deionized water (DDI) was added. The tubes were placed on a mechanical shaker for one hour
and then passed through a 0.45 [im filter with a vacuum. After extraction the samples were
refrigerated. Analysis with a TechniconTM Autoanalyzer was performed within 48 hours.
Mehlich-1 extractable P, Fe and Al were determined on all soil samples (Mehlich, 1953).
Five grams of soil was weighed into a 50 mL centrifuge tube and 20 mL of Mehlich-1 solution
release ammonium, increasing the pH within the microsite. Another is the presence of more acid
tolerant nitrifiers. The best possible explanation is that the lack of Al toxicity in organic and
some forest soils allows nitrification at even lower pHs (Paul and Clark, 1996). Temperature,
water potential, salinity and nutrient availability also affect nitrification rates (Myrold, 1999).
Aluminum negatively affects soil bacterial growth and overall numbers of rhizobium
(Glenn and Dilworth, 1991). The mechanisms which specifically harm the bacteria are
unknown. Soybeans and cowpeas have shown decreased levels ofN fixing capabilities when
grown in combination of low pH and toxic levels of Al (Mookherji and Floyd, 1991). A study
on forest soils reported decreased nitrification rates as potassium chloride extractable Al
increased (Gilliam et al., 2005).
Alexander (1980) proposed that acid precipitation affects nitrification more than other N
cycle phases. The effects of nitrification as impacted by acid precipitation were studied by
Bitton et al. (1985). Acid rain of pH 3.7 and 3.0 along with a control of 4.6 was applied to 75 m2
plots on a typical Florida sandy soil. Nitrification rates proved to be the most sensitive to the
acid rain and were reduced from 0.16 mg NO-3-N kg-1 d-1 on control plots to 0.08 and 0.06 mg
NO-3-N kg-1 d-1 for acid rain with a pH of 3.7 and 3.0, respectively.
There is little information on the impact that WTR application has on soil
microorganisms. In one study, six WTRs from South Africa were added to two soils at various
rates and soil respiration was monitored. In general, the soils amended with WTR showed
significant increases in soil respiration. The effects were less visible after time, indicating
increased microbial activity may not be a long-term result. Based on soil microbial indicators
alone, WTR could be applied at a 300 Mg ha-1 rate without negatively impacting soil quality
(Pecku et al., 2006).
Abstract of Thesis Presented to the Graduate School
of the University of Florida in Partial Fulfillment of the
Requirements for the Degree of Master of Science
ALUMINUM WATER TREATMENT RESIDUAL EFFECTS ON SOIL PHOSPHORUS
RETENTION AND FORAGE YIELD AND NUTRITIVE VALUE
By
Julie Lauter Driscoll
May 2008
Chair: Donald Graetz
Major: Department: Soil and Water Science
The health of aquatic systems is being threatened by the addition of phosphorus (P)
which causes increased eutrophication. Many Florida soils have naturally low capacities to
retain P, allowing for P loss to local waterways by leaching. In addition, P is often over-applied
when animal manures are used as fertilizers, which further increases the chances of P loss.
Water treatment residuals (WTRs) are by-products of the water purification process which
contain high amounts of amorphous aluminum (Al) or iron, giving them the capacity to retain P.
Land application of WTRs has been investigated mainly in greenhouse experiments to test the P
sorbing capacities of WTRs and to evaluate potential Al toxicity to plants.
The purpose of this project was to evaluate the effects of an Al-WTR on soil P retention,
soil microbial population, groundwater soluble reactive P (SRP) concentrations and forage yield
and nutritive value. Three WTR application rates (0, 1.5 and 3% dry weight by mass) and two
application methods (soil incorporation and surface applied) were tested on a bahiagrass
(Paspalum notatum) pasture in Ona, FL. Soils on the site had low P levels (A horizon = 2.1 mg
Mehlich-1 P kg-1) so triple superphosphate was added at 224 kg ha-1 to simulate high P loadings.
The addition of WTR showed a trend toward slightly higher pH. Water extractable P
concentrations decreased as WTR application rate increased, suggesting less P will be lost to
Codling, E.E., R.L. Chaney, and C.L. Mulchi. 2002. Biomass yield and phosphorus availability
to wheat grown on high phosphorus soils amended with phosphate inactivating residue I.
drinking water treatment residue. Comm. in Soil Sci. and Plant Anal. 33:1039-1061.
Codling, E.E., C.L. Mulchi, and R.L. Chaney. 2007. Grain yield and mineral element
composition of maize grown on high phosphorus soils amended with water treatment residual. J.
of Plant Nutrition 30:225-240.
Coleman, N.T. and G.W. Thomas. 1967. The basic chemistry of soil acidity. P. 1-41. In R.W.
Pearson and F. Adams (ed.) Soil Acidity and Liming. ASA, CSSA, and SSSA, Madison, WI.
Cox, A.E., J.J. Camberato, and B.R. Smith. 1997. Phosphate availability and inorganic
transformation in an alum sludge-affected soil. J. of Environ. Qual. 26:1393-1398.
Curtis, T.P. and W.T. Sloan. 2005. Exploring microbial diversity- A vast below. Science
309:1331-1333.
Dayton, E.A., and N.T. Basta. 2001. Characterization of drinking water treatment residuals for
use as a soil substitute. Water Environ. Res. 75:52-57.
Delhaize, Emmaneal and P.R. Ryan. 1995. Aluminum toxicity and tolerance in plants. Plant
Phys. 107: 315-321.
Edwards, A.C. 1991. Soil acidity and its interactions with phosphorus availability for a range of
different crop types. p. 299-305. In R.J. Wright et al. (ed.) Plant-soil interactions at low pH.
Kluwer Academic, Boston, MA.
Elliott, H.A., G.A. O'Connor, P. Lu, and S. Brinton. 2002. Influence of water treatment
residuals on phosphorus solubility and leaching. J. of Environ. Qual. 31:1362-1369.
FAWN (Florida Automated Weather Network). January 2008. http://fawn.ifas.ufl.edu/.
Fox, R.L., N.V. Hue, R.C. Jones, and R.S. Yost. 1991. Plant-soil interactions associated with
acid, weathered soils. p. 197-204 In R.J. Wright et al. (ed.) Plant-soil interactions at low pH.
Kluwer Academic, Boston, MA.
Foy C.D., R.L. Chaney, and M.C. White. 1978. The physiology of metal toxicity in plants.
Annual Review Plant Phys. 29:511-566.
Franzmeier, D.P., B.F. Hajek, and C.H. Simonson. 1965. Use of amorphous material to identify
spodic horizons. Soil Sci. Soc. of Am. J. 33:815-816.
Gallaher, R. N., C. O. Weldon and J. G. Futral. 1975. An aluminum block digester for plant and
soil analysis. Soil Sci. Soc. Amer. Proc. 39:803-806.
CHAPTER 1
INTRODUCTION
Nutrient enrichment was the leading cause of water degradation in the 1990s, polluting
approximately 45% of waters in the United States (USEPA, 2000). Nitrogen (N) and phosphorus
(P) are the main nutrients of concern; while they are naturally present in the environment, they
are also added as fertilizers in agriculture and home lawns and can enter aquatic systems through
runoff or leaching. Eutrophication occurs when increased levels of nutrients in aquatic systems
cause an influx of aquatic organisms and ultimately a decline in dissolved oxygen (Brady and
Weil, 2002). Under some eutrophic conditions, fishery, recreation and industrial water use is put
under restrictions and surface waters may no longer be used for drinking water (Sharpley et al.,
2001). Although N is necessary for the algae that cause the damages associated with
eutrophication, environmental concerns have been focused on P since it has been shown as the
limiting nutrient that most often causes accelerated impairment in aquatic systems (Sharpley et
al., 1994).
Florida has a large number of important water resources which contribute to many facets
of life: groundwater supplies 90% of the drinking water in the state, sport fishing and bird
watching bring millions annually to the economy and aesthetic beauty can be appreciated by all.
In Florida's unique ecosystem, P from urban and agricultural activities has impacted many
aquatic systems. The transfer of P downstream to other systems has created far reaching impacts
(Reddy et al., 1999). For example, drainage water enriched with P from the Everglades
Agricultural Area drains into the Water Conservation Areas and has caused eutrophic conditions.
The movement of P into freshwater bodies may lead not only to eutrophication but also generally
unhealthy biological conditions.
Table 3-1. Dates and descriptions of field activities.
Aug. 15-17, 2006 Laid plot perimeters and collected soil samples.
WTR delivered from Manatee County Water Treatment Plant. WTR
Sept. 9, 2006 sieved to 0.64 cm.
Initial forage samples taken. Moisture content of sieved WTR
Jan. 4, 2007 determined.
Plots mowed and forage removed. WTR weighed into bags for
Jan. 5, 2007 application.
Jan. 11, 2007 Al-WTR applied to plots with a drop fertilizer spreader
WTR incorporated into necessary plots. Fertilizer combination spread
Jan. 12, 2007 (34-0-60).
March 26, 2007 Wells placed in the middle of plots 60 cm deep.
Nitrification soil samples collected and then TSP applied at 224 kg ha'
April 23, 2007 per plot.
May 24, 2007 Forage harvested. Fertilizer combination spread (34-0-60).
June 20, 2007 Forage harvested. Fertilizer combination spread (34-0-60).
July 20, 2007 Forage harvested. Fertilizer combination spread (34-0-60).
Second soil samples collected. Forage harvested. Fertilizer combination
Aug. 20-21, 2007 spread (34-0-60).
Nitrification soil samples and groundwater samples collected. Forage
Sept. 20, 2007 harvested.
Oct. 2, 2007 Groundwater samples collected.
Water Treatment Residual
An Al-WTR was selected for field testing because of its effectiveness in controlling P
leaching when soluble P was in contact with the WTR. Aluminum WTR was acquired from the
Manatee County Water Treatment Plant in Bradenton, FL. Previous studies compared an Al-
WTR from this plant to an Al-WTR from Okeechobee, FL, and to other various soil
amendments. The ability of each amendment to sorb P and prevent leaching and runoff was
evaluated. The other amendments were not recommended for an assortment of reasons including
ineffective P sorbing capacities, high concentrations of potentially detrimental nutrients, threats
of soil salinity and high cost. The Manatee WTR was recommended over the Okeechobee WTR
for field evaluation because of its drier condition, ease of handling and the large quantities
values did not greatly vary and showed similar trends. Thus, the Mehlich-1 extractable
concentrations used can give a good indication of the SPSC values.
Soil Nitrification Potential
Since the microbial nitrification process in soils is sensitive to a variety of environmental
factors, it was used as an indicator to evaluate any adverse effect that the Al-WTR might have on
the soil microbial population. The assay used a solution of (NH4)2SO4 buffered at pH 7.2.
Preliminary studies showed essentially no nitrate production when this test was first used.
Because the soil had a relatively low initial pH (4.6), it was hypothesized that the buffered
(NH4)2SO4 solution added to the soil did not have enough buffering capacity to overcome the
low soil pH and the pH of the solution dropped to levels where microorganisms could not reach
their optimum nitrification potential. This was confirmed by a subsequent test that showed the
initial solution pH of 7.2 drop to 5.2 after addition of the soil. The pH of the soil was considered
to be a limiting factor in this study so the assay was rerun with newly collected soil and the
added solution contained a higher concentration of phosphate buffer to prevent the pH from
dropping.
After increasing the buffer strength of the solution, nitrification potential increased as the
application rate of WTR increased, especially in the surface applied treatments (Table 4-8 and
Fig. 4-10). The interactions between WTR application method and rate were not significant.
These results may be indicative of some of the possible benefits of WTR application
Ippolito et al. (2003) observed, including increased N and organic carbon availability and greater
aggregate stability. Potential nitrification rates were lower in the incorporated treatments
because the WTR was spread throughout the top 10-15 cm while the soil samples for this study
were collected only from the top 5 cm.
conditions present in the soil (pH < 5.2 and 6.2). Microbial community structure was analyzed
with denaturing gradient gel electrophoresis and diversity was not found to be adversely affected
by WTR application. This study concluded that WTRs could be applied to these specific soils at
rates up to 300 Mg ha-1 without negatively impacting the microbial community.
In another study highlighting the effects of Al-WTRs on plant decomposition, C-labeled
soybean leaves and stems were added to two soils amended with WTR at rates up to 1% dry
weight by mass. The Al-WTR did not affect decomposition of either the plant material or soil
organic matter. In comparison, an iron rich residue (not Fe-WTR) decreased decomposition rate,
likely because of pH or salinity changes caused by the amendment (Sikora, 2004).
Both of the above studies showed similar results as found in our study: Al-WTRs did not
cause adverse effects on the biological functions of soil as measured by soil microbial functions.
These two studies were performed under laboratory conditions while the Ona study employed
soil and WTR samples taken from field conditions.
Soluble Reactive Phosphorus in Groundwater
Because of drought conditions during the study period, wells contained sufficient
groundwater for sampling only during a period from mid-September to mid-October (Figure 4-
11). Samples were obtained at two dates during this period: September 20, 2007, and October 2,
2007.
method for all harvests (Tables 4-16). Surface applied WTR resulted in lower tissue P
concentrations than when WTR was incorporated. The surface applied treatments have nearly all
of the applied WTR still present close to the ground surface where most of the bahiagrass roots
are located. This higher concentration of WTR may be adsorbing P that would otherwise be
available for plant uptake. Plant maturity may also partially explain why the less mature plants
of the incorporated treatments showed higher tissue P. Less mature plants take up nutrients at a
quicker rate than more mature plants. For instance, in sorghum when half the plant mass had
been produced 60% of P, 70% of N and 80% of K had already been taken up (Vanderlip, 1993).
The surface applied controls may have been affected by the unusually large amount of P applied
and thus were not lower than the incorporated controls of less maturity. Application rate was
also significant at each harvest. However, there were differences between the 35 and 70 Mg ha-1
rates for only the July and August harvest dates.
Table 4-16. The p-values for the forage tissue P data using ANOVA and general mean contrast
(single degree of freedom).
Month Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
January 0.4676 0.7058 0.4934 0.5668 0.5519
May 0.0123 <.0001 0.0743 <.0001 0.1707
June 0.0032 0.0002 0.8144 <.0001 0.1113
July 0.0029 <.0001 0.0194 <.0001 0.0156
August 0.014 <.0001 0.0004 <.0001 0.0098
September 0.0208 <. 0001 0.039 <. 0001 0.1068
t Values italicized are significant at a = 0.05.
ft January data was collected before any amendments were applied. May through September
data was collected after WTR and TSP application.
Groundwater
Groundwater samples were analyzed for soluble reactive P (SRP) within 48 hours of
collection. Twenty milliliters of the sample was filtered through a 0.45im filter before analysis.
Samples were analyzed using a TechniconTM Autoanalyzer by EPA Method 365.1.
Forage
All forage samples were dried and then ground to 2 mm with a Wiley mill. Neutral
detergent fiber content (NDF), N (crude protein), P, Al, Ca and Mg were determined for forage
samples.
Neutral detergent fiber estimates the plant's cell wall contents and is the best indicator of
how much forage an animal will potentially consume. To determine the NDF, 0.50 g of grass
was weighed into a bag, the weight was recorded and the bag sealed (Golding et al., 1985). The
sample was then boiled in a neutral detergent solution for one hour leaving the cell wall
constituents. The bags were soaked in acetone and placed in an oven for 2-3 hours to complete
the drying process. The dry bags were weighed and the NDF was reported on a dry matter basis.
Tissue nutrient content (N, P, Al, Fe, Mg and Ca) was determined using a modification of
the total Kjeldahl method (Gallaher et al., 1975). Samples were weighed to 0.25 g and placed in
75 mL volumetric glass tubes. A catalyst (containing 9:1 K2S04:CuSO4), 6 mL of concentrated
H2SO4 and 2 mL of H202 were added and samples were digested at 3750C for at least 4 hours.
After samples cooled they were brought to volume with DDI water, mixed thoroughly, filtered
and stored in 20 mL scintillation vials. Nitrogen and P in the digestate was determined by
semiautomated colorimetery using a TechniconTM Autoanalyzer by TKN Method 351.2. Crude
protein was calculated by multiplying the amount of N in the sample by 6.25. Aluminum, Fe,
Mg and Ca were determined by atomic adsorption spectrophotometry.
Calcium and Magnesium
Table 4-18. The p-values for the forage tissue Ca and Mg data using ANOVA and general mean
contrast (single degree of freedom).
Nutrient Month Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
July 0.9331 0.0185 0.7056 0.0159 0.0958
Calcium August 0.0015 0.2582 0.7376 0.1923 0.31
September 0.005 0.0472 0.5771 0.0153 0.816
July 0.6282 0.7173 0.1722 0.5842 0.5539
Magnesium August 0.0657 0.0304 0.0668 0.0163 0.2267
September 0.6684 0.8677 0.1439 0.6497 0.7927
t Values italicized are significant at a = 0.05.
ft July through September data was collected after WTR and TSP application.
Tissue Ca concentrations for July through September samples showed little variation
ranging from 0.34% to 0.40% (Table 4-19) but did experience some effects due to application
method (August and September) and rate (July and September) (Table 4-18). Surface applied
WTR treatments had slightly higher tissue Ca concentrations than incorporated treatments.
Treatments amended with WTR had higher tissue Ca concentrations. Decreased Ca uptake was
not observed due to WTR application. Magnesium concentration was only affected in August by
application rate (Table 4-18). The surface applied control treatment was higher than all other
treatments and may be an anomaly (Table 4-20). It does not appear cation uptake is being
negatively affected by WTR application rate but may need to be monitored in the future.
Table 4-19. Tissue Ca percentages for July through September.
July August September
Treatment --------------------Tissue Ca %-----------------------------
0 0.353 a 0.353 c 0.333 b
35 I 0.360 a 0.355 bc 0.365 ab
70 I 0.385 a 0.373 abc 0.368 a
0 S 0.343 a 0.383 abc 0.373 a
35 S 0.370 a 0.393 ab 0.392 a
70 S 0.388 a 0.395 a 0.385 a
f Treatments with similar letters show no difference for that column by Waller-Duncan (p<0.05).
4-16 The p-values for the forage tissue P data using ANOVA and general mean contrast
(single degree of freedom ). ...................................................................... ....................70
4-17 T issue P content. .......................................................................................................... 7 1
4-18 The p-values for the forage tissue Ca and Mg data using ANOVA and general mean
contrast (single degree of freedom ). .................................................................................73
4-19 Tissue Ca percentages for July through September. ................... .................73
4-20 Tissue Mg percentages for July through September .......................................................74
restriction of root growth which later inhibits plant growth (Delhaize and Ryan, 1995). The
obstruction of root growth can cause the plant to experience less water and nutrient uptake since
roots do not penetrate the soil as extensively (Strid, 1996).
Aluminum toxicity can be minimized by adding various solutes to the solution. Hydroxyl
ions or alkaline salts reduce Al activity instead of Al concentrations but prove to be helpful
nonetheless. Raising the solution pH also decreases Al toxicity (Parker et al., 1989). While Al
toxicity is strongly affected by pH, the amount of extractable Al in soil and soil solution is
partially determined by mineralogy and the amount of weathering (Fox et al., 1991). For
instance, solution Al in an Ultisol had toxic values around 0.62 mg L-1 while Al concentrations
of an Oxisol were around 0.05 mg L-1 under the same growing conditions. Mineralogical
structure helps to explain these differences in Al solubility. More highly weathered soils
normally have lower Al solubility.
Edwards (1991) studied interactions between soil acidity, liming and P additions to
spring barley, winter wheat, woilseed rape and grass common to Scotland. Yield reductions
were witnessed with increases in acidity. By maintaining P soil levels adequate for crop growth,
the effects of Al toxicity were noticeable at pH values below the optimal growing conditions of
5.5.
In a study conducted by Dayton and Basta (2001), WTR was considered as a substitute
for the top layer of soil. Many properties were analyzed including cation exchange capacity,
nutrient levels, plant available water and potential toxicities. The only possible toxicity
problems pertained to levels of nitrite which if greater than 10 mg kg-1 are considered toxic to
plants. Water soluble Al levels fell below the regulatory value of 1.0 mg L-1 in all of the 14
WTRs tested.
LIST OF TABLES
Table page
3-1 Dates and descriptions of field activities. ............................................... ............... 34
3-2 Depths to the bottom of the soil horizons sampled. The A horizon was sampled from
0-8 cm and 8 cm to the bottom of the horizon................. ............................................. 36
4-1 General characteristics in soil samples taken prior to amendment application (August
2006) averaged for all plots. ..................................................................... ...................42
4-2 General characteristics of the Manatee County Al-WTR.................. .......... .........43
4-3 The p-values for the pH data using ANOVA and general mean contrast (single
degree of freedom )............................... .. ............................................. ...................43
4-4 Soil pH averages for incorporated and surface applied treatments 8 months after
W T R application ............................................................................................ 43
4-5 The p-values for the WEP data using ANOVA and general mean contrast (single
degree of freedom ) ................. .................................................... ............................ 45
4-6 The p-values for the Mehlich-1 data using ANOVA and general mean contrast
(single degree of freedom ). ...................................................................... ....................49
4-7 The p-values for the SPSC data using ANOVA and general mean contrast (single
degree of freedom )............................... .. ........................................ . ...................55
4-8 The p-values for the nitrification potential data using ANOVA and general mean
contrast (single degree of freedom ). .................................... ........ ............ .......... 60
4-9 The p-values for the groundwater SRP data using ANOVA and general mean
contrast (single degree of freedom ). .................................... ........ ............ .......... 63
4-10 The p-values for the forage yield data using ANOVA and general mean contrast
(single degree of freedom ). ...................................................................... ....................66
4-11 Forage yield averages for incorporated and surface applied treatments............................66
4-12 The p-values for the forage NDF data using ANOVA and general mean contrast
(single degree of freedom ). ...................................................................... ....................67
4-13 Forage NDF percentage averages for incorporated and surface applied treatments. ........68
4-14 The p-values for the forage crude protein data using ANOVA and general mean
contrast (single degree of freedom ). .................................... ........ ............ .......... 69
4-15 Crude protein percentage averages for incorporated and surface applied treatments. ......69
--*-- Initial
-35 Mg / ha
- Control
-e 70 Mg / ha
A
(0-8 cm)
o A
N
c (8-21 cm)
o
I
E
0
B
Mehlich-1 Al of Incorporated Treatments (mg kg-1)
Figure 4-5. Mehlich-1 Al concentrations of incorporated treatments by soil horizon. The dashed
line represents the average concentration of all plots from the 2006 sampling before
amendment application. The other lines represent the WEP concentrations from the
2007 soil sampling after amendment application.
--* -- Initial
- 35 Mg / ha
-- Control
--70 Mg / ha
A
c (0-8 cm)
o i
N
0 A
I (8-21 cm)
o0 E
B
0
Mehlich-1 Al of Surface Applied Treatments (mg kg-1)
Figure 4-6. Mehlich-1 Al concentrations of surface applied treatments by soil horizon. The
dashed line represents the average concentration of all plots from the 2006 sampling
before amendment application. The other lines represent the WEP concentrations
from the 2007 soil sampling after amendment application.
capacity (SPSC) which would account for both previous P loadings onto a soil and the capacity
of the soil to retain additional P. They found that some Spodosols in Florida had low STP and
DPS values but also low sorption capacities so loading these soils with P based on these two
factors could pose environmental risks. The SPSC value would show potential risk of P
application for soils with low sorption capacities.
Phosphorus Retention by WTR
The ability of WTRs to sorb P has been tested to determine if it can be used as an agent to
prevent P runoff. Gallimore et al. (1999) applied WTR to plots fertilized with two poultry litters
at a rate of 6.72 Mg P ha-1. The WTR was applied at 44.8 and 11.2 Mg ha-1 on broadcast plots
and 44.8 Mg ha-1 on a buffer strip. After simulated rain events, surface runoff was collected,
analyzed and compared to control plots. Dissolved P was reduced 42.7% in the broadcast plots
at the 44.8 Mg ha-1 WTR rate and 45.9% in the buffer strips when compared to the control. Land
application of WTR did not increase soluble Al or sediment in the surface runoff.
Novak and Watts (2004) evaluated the use of WTRs for P retention on sandy coastal
plains that have a high susceptibility to P leaching. Soils obtained from North and South
Carolina were combined with several WTRs in blends of 0, 2.5, 5.0, 7.5 and 10.0% dry weight
by mass. A calcium chloride solution with P concentrations ranging from 5,000 to 30,000 mg P
L-1 was added to the soil and WTR mixtures. The WTRs proved to sorb 100% of P when the
solution contained between 5,000 and 10,000 mg P L-1. The Pmax value determined for the soils
alone was less than 1 mg P g-1 while the Pmax when WTR was incorporated with the soil ranged
from 1.7 to 8.5 mg P g-1 depending on the composition of the WTR used. From this study it was
concluded that WTR can increase a soil's P sorption values and prospectively help P from
moving to bodies of water.
about by tilling may only be temporary. Future sampling may be needed to show how
groundwater SRP is affected by WTR application. The incorporated treatments with WTR did
not have lower SRP concentrations than the surface applied treatments with WTR. This is likely
because the main P source was applied only at the surface and was not present throughout the
soil profile.
Silveira et al. (2006) showed that by incorporating WTR in the top 15 cm of manure
impacted soil, SRP concentrations in leachate were reduced more than if the WTR was
incorporated in the top 7.5 cm. Control samples had SRP concentrations of 38 mg L-1, while the
7.5 and 15 cm incorporated samples had SRP concentrations of 19 and 2 mg L-1 respectively.
Rew (2006) observed that WTR applied on the surface better controlled P in runoff, but that
incorporating WTR better controlled P in subsurface flow and leachate concentrations. Both of
these studies dealt with highly impacted soils which contained large amounts of P throughout the
profile due to excessive loads of manure, while the soils at the Ona study site were naturally low
in P and had a P source applied to the surface.
Forage Yield and Nutritive Value
The P sorbing characteristics of Al-WTRs help prevent P loss from leaching and runoff
but also raise concern for P availability to plants and potential Al toxicity effects. Thus, forage
yield and several forage quality parameters were closely observed to evaluate the effects of WTR
application on bahiagrass.
Forage Yield
Forage was harvested in January 2007 prior to application of any treatment to evaluate
uniformity of the study area. No differences across treatments in forage yield were observed
before WTR was applied. Yield averaged 5.8 tons ha-1 (Table 4-11).
CHAPTER FOUR
RESULTS AND DISCUSSION
Soil Properties
Initial Soil Characteristics
The soil on the experimental site was classified as Myakka fine sand based on soil survey
information (USDA-Soil Conservation Service, 1984). This was substantiated during the
sampling process. Twenty-two of the 24 plots had a spodic horizon within the one-meter depth
sampled.
Initial soil WEP and Mehlich-1 P average concentrations were relatively low and
decreased with depth through the A and E horizons and then Mehlich-1 P slightly increased in
the B horizons whereas WEP remained low (Table 4-1). An anomaly was found in the A
horizon (0-8 cm) between WEP and Mehlich-1 P concentrations which were 9.0 and 2.1 mg kg-1,
respectively. It is uncommon for WEP concentrations to be higher than Mehlich-1 P
concentrations because extracting P with an acid solution should extract more P than the water
extract. There is no logical explanation for this observation and it was not observed in post-
treatment sampling. Mehlich-1 P concentrations in the B horizons were approximately 30-fold
greater than in the A and E horizons. Mehlich-1 P concentrations less than 10 mg kg-1 in the A
horizon are considered very low for agronomic crops as well as bahiagrass and P fertilization
would be recommended for this site (Kidder et al., 2002). These concentrations are slightly
lower to those found in forage fields in South Florida (Graetz and Nair, 1995).
such as pasture soils have less P and so the possibility of P moving out of the system is less than
a highly impacted soil (Nair et al., 1999).
Soil Microorganisms
Microorganisms encompass only 1-3% of soil organic matter (Jenkinson, 1988) yet there
can be as many as 109 bacterial cells per gram of soil (Torsvik et al., 1990). Microorganisms
have many vital roles in the soil environment. They decompose organic matter thereby releasing
substantial amounts of nutrients. They are major constituents in biogeochemical cycles (carbon,
nitrogen, sulphur) and also help in environmental processes such as the degradation of pollutants
(Curtis and Sloan, 2005).
Soil microorganisms are considered an excellent biological soil quality indicator because
they respond quickly to environmental changes (Turco et al., 1994). They also play a vital role
in the cycling of N and C (Nannipieri et al., 1990). Specifically, nitrification is a functional
parameter in assessing soil quality because it is carried out by specialized bacteria which are very
sensitive to environmental changes, stress and toxic substances (Bollmann, 2006).
Nitrification, the reduction ofN to nitrate (NO3s), is normally a two-stage process with
ammonia being oxidized to nitrite, and then nitrite quickly being oxidized to NO3- (Prosser,
2007). Nitrification is affected by a number of factors which can interact to affect the overall
process. Nitrifiers must be present, and because they are aerobic organisms oxygen must also be
available. Nitrification is optimal when soils are near field capacity or when 60% of pore spaces
are filled with water. The most important regulating factor is substrate availability, namely
ammonium (Myrold, 1999). Soil pH is another influence on nitrification. Most soils below pH
4.5 have negligible nitrification rates because nitrifiers generally work best at neutral pH. It has
been shown however that nitrification can occur at pH below 4.0 in forest soils. One possible
explanation for nitrification under these low pH conditions is that N rich material in microsites
plants, improved aggregate stability and water retention, better aeration and drainage capacity
and increased cation exchange capacity for coarse textured soils.
Numerous studies have looked at the characteristics and capabilities of WTRs as a means
of controlling P loss from leaching. Most of these studies have taken place in a laboratory or a
greenhouse under controlled conditions. These methods are a good way to make initial
evaluations of the tested material. There is concern in the agricultural sector of using an
amendment containing Al and the effects it may have on crop growth and production. There is a
strong need to determine the effects WTR will have on soil P movement, plant P uptake and
possible Al toxicity problems under field conditions. This study will address these concerns
under field conditions.
The objectives of this study are to:
1. Evaluate the effects of Al-WTR application method and rate on P forms in the soil
2. Determine the effects of Al-WTR application on the soil microbial population
3. Examine P leaching through groundwater monitoring
4. Evaluate forage yield and nutritive value as affected by Al-WTR application method
and rate
Villapando, R.R. and D.A. Graetz. 2001. Water table effects on phosphorus reactivity and
mobility in a dairy manure-impacted spodosol. Ecol. Eng. 18:77-89.
Zhang, M.K., Z.L. He, D.V. Calvert, P.J. Stofella, Y.C. Li, and E.M. Lamb. 2002. Release
potential of phosphorus in Florida sandy soils in relation to phosphorus fractions and adsorption
capacity. J. of Environ. Sci. Health A Tox. Harzard. Subst. Environ. Eng. 37(5): 793-809.
12 -
-0 Sept. 20
S10 Oct. 2
E
a_ 8
>a
S 6 AB a
AB b ab
4 4 -
-b
T 2
Incorporated Incorporated Incorporated Surface Surface Surface
OMg /ha 35Mg/ha 70 Mg /ha 0 Mg /ha 35 Mg /ha 70 Mg /ha
Application Method and Rate
Figure 4-12. Water SRP concentrations across treatments for two sampling dates in 2007.
tTreatments with similar letters and casing show no significant difference by Waller-Duncan
(p<0.05).
The process of incorporation impacted the groundwater SRP concentrations. The surface
applied control treatments had almost twice as high SRP concentrations than all of the
incorporated treatments, including the incorporated control treatments. As previously
mentioned, tilling destroys macropores so water movement is greater through no-till systems.
Shipitalo et al. (2000) studied the leaching of anions and cations in tilled and no-till systems by
using strontium bromide hexahydrate (SrBr2 6H20) as a tracer. The Br was leached more
quickly in the no-till systems initially but overall anion loss after 2 years was not different
between tilled and no-till systems. Thus there may have been greater leaching of SRP in the
surface applied treatments, possibly explaining why the surface applied control had greater
concentrations than the incorporated control.
The SRP concentrations for the incorporated treatments showed no differences. The
effects due to tilling seem to have prevailed over any possible effects resulting from WTR
application that were seen in the surface applied treatments. However, the conditions brought
CHAPTER THREE
MATERIALS AND METHODS
Site Description and Experimental Design
The study was performed on an established bahiagrass (Paspalum notatum) pasture at the
Range Cattle Research and Education Center (RCREC) in Ona, FL (27026' N, 81055' W). Main
plots (15.24 X 6.10 m) were divided into subplots (3.05 X 6.10 m) and separated by 3.05 m
buffer strips (Fig. 3-1). A randomized block, split-plot design used application method (soil
incorporation and surface applied) as the main plot and WTR rate (0, 35 and 70 Mg ha-1) as the
subplot. Each treatment was replicated four times. Aluminum WTR was incorporated in the top
10-15 cm of soil in the designated plots by disking. The bahiagrass was allowed to grow in from
the existing tilled-in material. Elliott et al. (2002) determined practical and effective WTR
application rates to be between 20 and 100 Mg ha-1 through column leaching studies and thus
rates for this study were chosen within this range.
DII IDI ..
N
M1 DII
DII lID
Figure 3-1. Plot design and layout. The shaded regions indicate surface applied treatments and
non-shaded regions indicate incorporated treatments. White boxes are the controls,
light gray boxes are the 35 Mg ha-1 WTR application rate and the darker gray boxes
are the 70 Mg ha-1 WTR application rate.
CHAPTER 5
CONCLSUIONS
The use of Al-WTRs has been proposed as a means to help reduce P runoff and leaching
from agricultural land where soils are loaded with high amounts of P or have a low capacity to
retain P. Many laboratory and greenhouse studies have been conducted to evaluate the
efficiency ofWTRs to retain P and to ascertain the potential for Al toxicity to plants. This field
study evaluated the effects on soil P retention and movement when applying an Al-WTR
followed by a heavy application of P to a soil with a low capacity to retain P. High amounts of P
were added in the form of triple superphosphate (TSP) to simulate high P loadings that may
result from biosolid or manure application. An Al-WTR was added at 1.5 and 3.0% of the dry
weight of soil to a bahiagrass pasture in South Florida. Half of the plots were disked in order to
incorporate the amendment in the top 10-15 cm. Soil samples were taken from each horizon to a
depth of one meter 8 months after WTR application. Soil pH, water extractable P, Mehlich-1 P,
Al and Fe, and the soil P storage capacity (SPSC) were measured. The soil microbial population
was monitored with a nitrification assay. Groundwater samples were collected from wells placed
above the spodic horizon in each plot. Forage samples were collected every 30 days for 5
months to monitor for toxicity effects. Yield, neutral detergent fiber (NDF), crude protein and
tissue analysis including P, Al, Ca and Mg content were observed.
Soil pH increased slightly with WTR application, thereby creating better conditions for
bahiagrass growth and also decreasing the threat of Al toxicity. The addition of WTR reduced
water extractable P concentrations in the A and E horizons. Mehlich-1 P concentrations showed
no increases due to WTR application, but increased from previous conditions only because of the
large amount of P added as TSP. Mehlich-1 Al concentrations and SPSC increased as WTR
application rate increased. The soil's capacity to retain P increased even after heavy P loadings
TABLE OF CONTENTS
page
A C K N O W L E D G M E N T S ..............................................................................................................4
L IS T O F T A B L E S .................................................................................7
LIST O F FIG U RE S ................................................................. 9
ABSTRACT ........................................... .. ......... ........... 10
CHAPTER
1 INTRODUCTION ............... .............................. ............................. 12
2 L ITE R A TU R E R E V IE W ......................................................................... ........................ 16
P hosphorus... . .............................................................16
Phosphorus Retention by W TR ......................................................................... ............... 18
W TR -Induced P D efficiency in Plants.......................................................... ............... 22
A lu m inu m .................. ................. .................................................................................... 2 4
Phosphorus and W after M ovem ent in Spodosols ........................................ .....................27
Soil Microorganisms................... ....... .. .... ..... ..................28
B a h ia g ra ss ................... ...................3...................0..........
3 M A TER IA L S A N D M ETH O D S ........................................ .............................................32
Site D description and Experim ental D esign...................................... .................................. 32
W after T reatm ent R esidual ............................................................................ ....................34
S o il S am p lin g ............................................................................................... ............... 3 5
Groundwater Sam pling ..................................................... ............ ............ 36
A analytical M ethods............ ...... ......................... ..........37
S o il ..............................................................................................3 7
N itrification Potential ...................... ...................... ... ......... .... ....... 38
G ro u n d w after ........................................................................................3 9
F o ra g e ..................................................................................................3 9
Q quality A assurance and Q quality C ontrol...................................................................... ...... 40
Statistical A analysis .............................................40
4 RESULTS AND D ISCU SSION .................................................. ............................... 41
Soil Properties ............................ ............... 41
Initial Soil C characteristics ....................... ............................................ ............... 41
Aluminum Water Treatment Residual Characteristics................ ..............42
W ater E xtractable P hosphorus ............................................................. .....................45
M ehlich-1 E xtractions ............... ....................................................... ........ 49
Soil Phosphorus Storage C apacity........................................................ ............... 54
surface applied treatments (Figs. 4-3 and 4-4). This trend shows less Mehlich-P in the A (0-8
cm) horizon of the controls than WTR amended treatments but more Mehlich-1 P in the A (8-21
cm) and E horizons of the controls than WTR amended treatments. This suggests P is being
retained near the surface in the WTR treated soils and not moving lower down the soil profile.
This trend may be more significant with time as P will continually move downward in the
controls.
LIST OF FIGURES
Figure page
3-1 Plot design and layout............ .. .... ...................... ............... 32
4-1 The WEP concentrations of incorporated treatments by soil horizon ............. ...............47
4-2 The WEP concentrations of surface applied treatments by soil horizon .........................47
4-3 Mehlich-1 P concentrations of incorporated treatments by soil horizon...........................51
4-4 Mehlich-1 P concentrations of surface applied treatments by soil horizon.....................51
4-5 Mehlich-1 Al concentrations of incorporated treatments by soil horizon .......................53
4-6 Mehlich-1 Al concentrations of surface applied treatments by soil horizon ...................53
4-7 Soil P storage capacity of incorporated treatments by soil horizon...............................56
4-8 Soil P storage capacity of surface applied treatments by soil horizon............................. 56
4-9 Differences in SPSC for incorporated and surface applied treatments ...........................58
4-10 Nitrification potential of treatments for soil samples taken on September 20, 2007.........60
4-11 Water depth data from the pressure transducer.........................................62
4-12 Water SRP concentrations across treatments for two sampling dates in 2007 .................64
Table 3-2. Depths to the bottom of the soil horizons sampled. The A horizon was sampled from
0-8 cm and 8 cm to the bottom of the horizon.
Soil Horizon Average Depth (cm)
A 8
A 21
E 54
Bw 75
Bh 83
Bt > 100
Soil samples were collected on April 23, 2007 and September 20, 2007 to determine
nitrification potential. In these samplings, the top 5 cm of the A horizon were placed in sampling
bags and stored in a cooler. A 1.3 cm diameter stainless steel probe was pre-marked to 5 cm and
two samples from each quartile of the plot were collected.
Forage Sampling
An initial forage sampling was conducted in January 2007 before any treatments were
applied. Two 51cm2 tracts were used to take forage samples from each plot. The grass was cut
to approximately 3 cm above the ground with hand shears. Plots were harvested every 30 days
from May to September, 2007, with a flail mower and a dump wagon attachment to collect and
weigh harvested forage. Hand drawn samples at each harvest were dried at 105F, moisture
content calculated and sieved to 2 mm for analysis. After each harvest, plots were fertilized with
ammonium nitrate and potassium chloride at rates of 89.7 kg N ha-1 and 44.9 kg K20 ha-1,
respectively.
Groundwater Sampling
A 60 cm PVC well was placed above the spodic horizon in the center of all plots. There
were slits in the lower 10 cm to allow water to enter the well. The goal was to collect
groundwater samples when a perched water table existed to observe concentrations of P
leaching. Due to an unusually dry early summer, samples were not able to be collected until late
LIST OF REFERENCES
Alexander, M. 1980. Effects of acid precipitation on biochemical activities in soil. p. 47-52. In
Proceedings of the International Conference on Ecological Impact of Acid Precipitation,
Sandefjord, Norway. SNSF Project, 47-52.
Allen, L.H. 1987. Dairy-siting criteria and other options for wastewater management on high
water-table soils. Soil Crop Sci. Soc. ofFl. Proc. 47:108-127.
Agyin-Birikorang, S., G.A. O'Connor, L.W. Jacobs, K.C. Makris, and S.R. Rinton. 2007.
Long-term phosphorus immobilization by a drinking water treatment residual. J. of Environ.
Qual. 36:316-323.
Ann, Y., K.R. Reddy, and J.J. Delfino. 1999. Influence of chemical amendments on phosphorus
retention in a constructed wetland. Ecol. Eng. 14:213.
Arthington, J.D. and W.F. Brown. 2005. Estimation of feeding value of four tropical forage
species at two stages of maturity. J. of Anim. Sci. 83:1726-1731.
Bitton, G. Volk, B.G., Graetz, D.A., Bossart, J.M., Boylan, R.A., and years, G.E. 1985. Effect of
acid precipitation on soil microbial activity: II. field studies. Journal of Environmental Quality
14:69-71.
Board on Agriculture. 1980. Mineral Tolerance of Domestic Animals. Subcommittee on
mineral toxicity in animals, Committee on animal nutrition. National Academy of Sciences,
Washington, D.C.
Botcher, A.B. and R.D. Rhue. 1984. Fertilizer management key to a sound water quality
program. EDIS. http://edis.ifas.ufl.edu/CIR-816.
Bollmann, Annette. 2006. Nitrification in soil. p. 136-179. In J. Bloem et al. (ed.)
Microbiological methods for assessing soil quality. CABI Publishing, Cambridge, MA.
Brady, N.C. and R.R. Weil. 2002. Elements of the Nature and Properties of Soils. Prentice
Hall, Upper Saddle River, NJ.
Burgora, B.A. 1989. Phosphorus spatial distribution, sorption, and transport in a Spodosol.
Ph.D. Dissertation. University of Florida.
Chambliss, C.G. and M.B. Adjei. 2006. Bahiagrass. EDIS. http://edis.ifas.ufl.edu/SS-AGR-36.
Codling, E. E., R. L. Chaney, and C. L. Mulchi. 2000. Use of aluminum and iron rich residue to
immobilize phosphorus in poultry litter and litter amended soils. J. of Environ. Qual. 29:1924-
1931.
which has low solids content (1-2%). Surface applied alum sludge had little effect on P
availability while incorporating the sludge into the entire soil volume did show a decrease in P
availability in the significantly decreased dry matter yields and plant P uptake. When the sludge
was incorporated, Al compounds retained soil P that was otherwise loosely bound soil P and
available to the wheat. It was suggested that higher P fertilizer requirements will be needed on
soils amended with alum sludge.
An Fe-WTR was amended with P to determine if adding P could prevent P deficiencies
in plants that may be caused by the WTR. The Fe-WTR was also amended with a cationic
polymer, Magnafloc 572C, before being applied to sorghum-sudangrass. The amount of P
needed to make the Fe-WTR a supplier of P was only 14.6 to 19.4 g P kg-1. This low P rate was
partially a result of P release from the cationic polymer. The cost of adding P to WTRs to make
them a supplier of P will vary depending on the amount of Fe or Al, but in this study it was more
economical than placing the WTR in a landfill (Hyde and Morris, 2004).
In a rainfall simulation, the effects of an Al-WTR at a 2.5% dry weight by mass
application rate were observed for stargrass (Cynodon spp). Shoot growth, root biomass, and
forage P, N and Al concentrations were not affected by the WTR (Rew, 2006). An Al-WTR was
applied in combination with poultry litter manure under field conditions to determine if the WTR
amendment would affect the yield and nutrient concentration of corn (Zea mays L.). The poultry
litter was amended with WTR at rates of 0, 5.6 and 11.2 Mg ha-1 before being applied to the soil.
The two sites used for the study were classified as an Ultisol and an Entisol. Corn grain yield
and mineral nutrients (Zn, Mn, Fe, Ca, Mg, and K) in the leaves and grain were not affected by
the addition of WTR. Tissue P levels remained above adequate growth levels for corn (2.0 g
kg-') and were similar to control plot leaf P concentrations, indicating adequate levels of
- -- Initial
A 35 Mg / ha
- Control
- 70 Mg / ha
WEP of Incorporated Treatments (mg kg-1)
Figure 4-1. The WEP concentrations of incorporated treatments by soil horizon. The dashed line
represents the average WEP concentration of all plots from the 2006 sampling before
amendment application. The other lines represent the WEP concentrations from the
2007 soil sampling after amendment application.
- -Initial
-A-35 Mg / ha
--Control
--70 Mg / ha
WEP of Surface Applied Treatments (mg kg-1)
Figure 4-2. The WEP concentrations of surface applied treatments by soil horizon. The dashed
line represents the average WEP concentration of all plots from the 2006 sampling
before amendment application. The other lines represent the WEP concentrations
from the 2007 soil sampling after amendment application.
A
(0-8 cm)
A
(8-21 cm)
E
A
(0-8 cm)
A
(8-21 cm)
E
Alum (A12(SO4)3) has been used to reduce P in a variety of environmental conditions. It
has been applied in wetlands when sediments have been known to release P (Ann et al., 1999)
and also to soils amended with poultry litter high in P (Shreve et al., 1995). The P retaining
capacities of alum were compared to an Al-WTR because of the high cost of alum. The Al-WTR
was nearly as effective as alum at reducing the amount of P released when combined with a
poultry litter. The advantages of the WTR include lower costs and less dissolved Al released
than alum (Makris et al., 2005).
Other amendments have also been studied for their ability to reduce P movement.
Haustein et al (2000) compared the efficiency of a WTR to a by-product of the commercial alum
production process, HiClay Alumina (HCA). An Al-WTR and HCA were applied at rates of 0,
2.2, 9.0, and 18 Mg ha-1 to plots with high to excessive soil test P levels (150 to 300 mg P kg-1
soil). Rainfall was simulated to produce runoff one day, one month, and four months after
application. The WTR was able to sorb P at a rate 20 times higher than HCA most likely
because WTR contained three times as much Al and more clay particles. This study also looked
at Al concentrations in the runoff The rain event after one day showed greater amounts of total
Al for the two highest WTR application rates than the control since the residue contained 47 g Al
kg- Runoff Al concentrations after one and four months did not differ from the controls. Thus,
controlling when WTR is applied may prevent greater levels of Al in runoff
Elliott et al. (2002) examined the ability of Al-WTR, Fe-WTR and Ca-WTR to prevent P
leaching in a sandy soil amended with biosolids and triple superphosphate (TSP). Soil columns
were set up with bahiagrass grown on an A horizon of Immokalee sand and an E horizon of a
Myakka series. WTR was applied at 56 Mg ha-1. The controls had 21% of total P leach from
TSP and 11% of total P leach from the Largo cake biosolid over four months. When WTR was
Rew (2006) did not observe changes in soil Mehlich-1 P before or after rainfall
simulations in which an Al-WTR was applied at rates up to 2.5% dry weight by mass. The soil
was impacted with heavy loads of manures and so had initially high P concentrations. Before the
simulations began, some treatments had the WTR mixed throughout the top 10 or 20 cm and no
Mehlich-1 P differences occurred due to this application method.
Mehlich-1 Al concentrations were higher in the A (0-8 cm) and A (8-21 cm) horizons
when the WTR was incorporated compared to surface applied (Table 4-6; Figs. 4-5 and 4-6).
Application rate of WTR affected Mehlich-1 Al concentrations in the A (0-8 cm), A (8-21 cm)
and E horizons. Additions of WTR did not change the soil Fe concentration due to the low Fe
concentrations in the Al-WTR (Table 4-6).
applied, the amount of leached P reduced to 3.5%, 2.5% and less than 1% of total applied P for
Fe-WTR, Ca-WTR and Al-WTR respectively, making the Al-WTR the most effective in
decreasing soluble P of the materials tested.
Miyittah-Kporgbe (2004) investigated the effects of application rate and the level of
mixing an Al-WTR with the soil on P leaching. Leaching rates determined through a column
study showed a reduction by 87 to 99.7% when Al-WTR was mixed completely with 15 cm of
soil; partial mixing with 7.5 cm of soil only resulted in 40 to 58% less leaching than control
columns with no applied WTR. More P retention was accredited to more contact with the
amendment. Runoff from rainfall simulations showed that when Al-WTR was surface applied,
soluble P concentrations were less than the critical threshold of 0.03 mg L-1. The leachate
concentrations when WTR was surface applied were above the critical threshold because of
limited contact with the amendment. These studies showed that higher surface application rates
can be as effective as using lower quantities of Al-WTR mixed throughout the soil profile.
Therefore, Al-WTR should be applied and distributed according to anticipated P losses. Surface
applications will help prevent runoff losses and mixing the amendment will help prevent
leaching.
In a study by Silveira et al. (2006) the efficiency of WTR mixed within the entire profile
was evaluated by observing soluble reactive P (SRP) concentrations in leachate. WTR was
added to a manure-impacted Immokalee soil at rates of 0, 25, 50 and 100 g kg-1. The WTR in
the soil columns was either left unincorporated or mixed within the top 7.5 or 15 cm. Water
extractions through the columns were analyzed for SRP. Control samples had an average SRP
concentration of 38 mg L-1. In columns with WTR in the top 7.5 cm, SRP decreased to 19 mg
L-1 and when WTR was in the entire 15 cm SRP further declined to 2 mg L-1. Thus, results
Neutral detergent fiber percentages did not change due to WTR application rate over the
course of the study (Table 4-12). Values were only affected by application method in the June
and August harvests. In June, the surface applied treatments had higher NDF percentages than
the incorporated treatments. However, the control was not different than the treatments
receiving Al-WTR suggesting that WTR was not a factor in causing the decrease in NDF. The
August NDF averages for incorporated and surface applied treatments differed less than 1%, and
were not different when analyzed using the Waller-Duncan test (Table 4-13). Overall, NDF was
not impacted by WTR application rate.
Table 4-13. Forage NDF percentage averages for incorporated and surface applied treatments.
Application Method January May June July August September
---------------------------- Neutral Detergent Fiber %----------------------------
Incorporated 76.18 a 68.85 a 68.15 b 70.23 a 70.03 a 73.95 a
Surface Applied 76.14 a 67.58 a 70.84 a 70.63 a 70.68 a 74.84 a
f Treatments with similar letters show no difference for that column by Waller-Duncan (p<0.05).
The NDF percentages found in this study were similar to those found for bahiagrass in
other studies in Florida. A study examining bahiagrass NDF based on organic matter percentage
at the RCREC in Ona, FL, showed values of 76% for both 4 and 10 week maturity periods
(Arthington and Brown, 2005). Samples of coastal bermudagrass taken in October and
November from dairies in the Suwanee River Valley showed NDF content ranging from 78 to
85% (Staples et al., 2003).
Crude Protein
There were no pretreatment (January) differences among treatments in bahiagrass crude
protein percentage (Tables 4-14). There were also no differences in crude protein content of
forage due to WTR application rate in post-treatment harvests. There was an effect due to
application method as surface applied treatments had higher percentages than the incorporated
treatments in May; in June and July the incorporated treatments had higher percentages than the
BIOGRAPHICAL SKETCH
Julie Lauter Driscoll was born in 1984. She is the third daughter of Michael and Connie
Driscoll and was born and raised in Fort Pierce, FL. Coming from a long line of Gators, she
began her undergraduate degree in August 2002 at the University of Florida where she decided
to major in soil and water science. She worked at the Indian River Research and Education
Center in Fort Pierce for three summers where she completed an internship and an independent
work study in a soil toxicology lab. It was these experiences where she discovered a desire to
protect agriculture and the environment and decided to pursue her Master of Science in the
summer of 2006. Julie worked to prevent phosphorus leaching from impacting surface and
ground waters at a field study in Ona, FL.
Gallimore, L.E., N.T. Basta, D.E. Storm, M.E. Payton, R.H. Huhnke, and M.D. Smolen. 1999.
Water treatment residual to reduce nutrients in surface runoff from agricultural land. J. of
Environ. Qual. 28: 1474-1478.
Gilliam, F.S., N.L. Lyttle, A. Thomas, and M.B. Adams. 2005. Soil variability along a nitrogen
mineralization and nitrification gradient in a nitrogen-saturated hardwood forest.
Soil Sci. Soc. of Am. J. 69:247-256.
Glenn, A. and M. Dilworth. 1991. Soil acidity and the microbial population: survival and
growth of bacteria in low pH. p. 567-579. In R.J. Wright et al. (ed.) Plant-soil interactions at
low pH. Kluwer Academic, Boston, MA.
Godsey, C.B., G.M. Pierzynski, D.B. Mengel, and R.E. Lamond. 2006. Changes in soil pH,
organic carbon, and extractable aluminum from crop rotation and tillage. Soil Sci. Soc. of Am. J.
71:1038-1044.
Golding, E. J., M. F. Carter, and J. E. Moore. 1985. Modification of the neutral detergent fiber
procedure for hay. J. Dairy Sci. 68:2732-2736.
Graetz, D.A. and V.D. Nair. 1995. Fate of phosphorus in Florida spodosols contaminated with
cattle manure. Ecol. Eng. 5:163-181.
Graetz, D.A. and V.D. Nair. 1999. Inorganic forms of phosphorus in soils and sediments. p.
171-186. In K.R. Reddy et al. (ed) Phosphorus biogeochemistry in subtropical ecosystems:
Florida as a case example. Lewis Publishers, Boca Raton, FL.
Harris, W.G., R.D. Rhue, G. Kidder, R.B. Brown, and R. Littell. 1996. Phosphorus retention as
related to morphology of sandy coastal plain soil materials. Soil Sci. Soc. of Am. J. 60:1513-
1521.
Hart, S.C., J.M. Stark, E.A. Davidson, and M.K. Firestone. 1996. Nitrogen mineralization,
immobilization and nitrification. P. 958-1018. In R.W. Weaver, et al. (ed.) Methods of soil
analysis, microbiological and biochemical properties. SSSA, Madison, WI.
Haustein, G.K, T.C. Daniel, D.M. Miller, P. Moore, and R.W. McNew. 2000.
Aluminum-containing residuals influence high-phosphorus soils and runoff water quality. J. of
Environ. Q. 29:1954-1959.
Havlin, J.L., D.E. Kissel, L.E. Maddux, M.M. Claassen, and J.H. Long. 1990. Crop rotation and
tillage effects on soil organic carbon and nitrogen. Soil Sci. Soc. of Am. J. 54:448-452.
He, Z., H. Yao, D. Calvert, P. Stofella, X. Yang, G. Chen, and G. Lloyd. 2005. Dissolution
characteristics of central Florida phosphate rock in an acidic sandy soil. Plant Soil 273:157-166.
Heil, D.M., K.A. Barbarick. 1989. Water treatment sludge influence on the growth of
sorghum-sudangrass. J. of Environ. Qual. 18:292-298.
ACKNOWLEDGMENTS
I would first like to thank Dr. Don Graetz for all of his support in both the work on this
thesis and with the Agronomy and Soils Club. I also thank my committee members Dr. Dean
Rhue and Dr. Maria Silveira for all of their time and effort in helping me compile my work. I
would like to pay my respects to Dr. Martin Adjei who passed away shortly after this study
began. I also thank the Soil and Water Science Department for their input and guidance in every
aspect of my work, especially Dr. Nair, Dr. Ogram, Dr. Harris and Mace Bauer.
I extend my deepest thanks to Dawn Lucas for being my mom away from home and
helping with all of my laboratory analysis. I would also like to thank everyone in Lab 212 for
their advice, help with lab work and overall friendships: Myrlene, Lacey, Ryan, Brandon,
Leighton and Dan. I thank Richard Fethiere and the Forage Evaluation Support Lab for allowing
me to do so much of my forage work in your lab. Thank you to Bill Reves and Nancy Wilkinson
for help in analyzing samples. I thank Cindy Holley for all her efforts and help in coordinating
field work in Ona. Also, thanks go to all who helped work in field during those hot summer
months: TJ, Kenny, David and especially my Dad.
I would like to thank my family for all of their support and love throughout the years. I'll
finally be the "Dr. of Dirt" as you like to refer to me. Thanks also go to the Agronomy and Soils
Club advisors and members for teaching me to be a leader and for all of the lifelong friendships.
Finally, I would like to thank all my friends for two great years of graduate school. Hughes,
thanks for all the fun with biochem and sampling months; Miller, thanks for being my best friend
in the department and willingly participating in so many Gator events; Bri and Tom, thanks for
taking on regionals and the Appalachian Trail with me.
weight by mass in a wheat growth chamber experiment (Codling et al., 2002). Soil WSP
decreased as WTR rate increased for all three soils and was reduced 98 to 99% at the 5%
application rate when compared to the controls.
Mehlich-1 Extractions
Mehlich-1 P concentrations were affected by application method in the A (8-21 cm)
horizon (Table 4-6). The distribution of Mehlich-1 P in the soil profile for incorporated and
surface applied WTR treatments is shown in Figs. 4-3 and 4-4, respectively. As noted earlier for
WEP concentrations, the blanket application of TSP is responsible for the considerable increases
in Mehlich-1 P in 2007 compared to 2006.
Table 4-6. The p-values for the Mehlich-1 data using
degree of freedom).
Response Soil Horizon Method Rate
Variable
Mehlich-1 A (0-8 cm) 0.2794 0.4589
P A (8-21 cm) <.0001 0.0696
E 0.0811 0.1607
B 0.1635 0.3327
Mehlich-1 A (0-8 cm) 0.0099 <.0001
Al A (8-21 cm) 0.0005 <.0001
E 0.4174 0.038
B 0.7486 0.8271
Mehlich-1 A (0-8 cm) 0.0742 0.1745
Fe A (8-21 cm) 0.7233 0.8367
E 0.5292 0.2455
B 0.6607 0.0669
t Values italicized are significant at a = 0.05.
ANOVA and general mean contrast (single
-1
Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
0.8492 0.2202 0.9433
0.5926 0.0617 0.1762
0.8637 0.0616 0.8085
0.286 0.4761 0.1217
0.1267 <.0001 0.0026
0.0174 <.0001 0.0076
0.1199 0.0375 0.0825
0.6664 0.5454 0.9947
0.5916 0.1465 0.227
0.7284 0.6436 0.6983
0.613 0.114 0.5795
0.9241 0.0557 0.146
The only difference observed for Mehlich-1 P concentrations was in the A (8-21 cm)
horizon where the WTR incorporated treatments had greater concentrations than the surface
applied treatments (Table 4-7). This may be a result of the incorporation process since tilling did
not occur exactly at 8 cm where the A horizon was split for two samples. Mehlich-1 P
concentrations followed the same general trend for all treatments in both the incorporated and
Quality Assurance and Quality Control
Quality assurance and quality control (QA/QC) were met with 10% repeats and blanks
for each procedure. Quality control was determined by certified external standards and standard
calibration curves. A standard deviation of 10% was required for all repeats and quality control
checks.
Statistical Analysis
Statistical analysis was executed using SAS version 8.2 1999-2001 (SAS Institute Inc.,
Cary, North Carolina, USA). The Waller-Duncan procedure was used to compare the mean
values for application method and application rate. ANOVA was used to determine the
individual variable responses (application method and rate) and interactions between the
variables. A single degree of freedom was used to explore the general mean contrast between
application rates.
WTR-Induced P Deficiency in Plants
Phosphorus deficiency in plants is a concern when applying an Al based WTR. Because
of its chemical makeup and high concentration of Al, WTRs may sorb the P available to and
needed for plants. Codling et al. (2002) applied WTR to three different soils at rates of 0, 10, 25
and 50 g kg-1. After seven weeks, three cycles of wheat (Triticum aestivum L.) were grown. At
the 10 g kg-1 WTR application rate, biomass yield was not influenced for all the soils. Two of
the three soils showed lower biomass yields at the 25 and 50 g kg-1 WTR application rates.
These two soils had a greater sand content than the unaffected soil. Tissue P concentration fell to
deficient levels (<1.5 g kg -1) at the 25 and 50 g kg-1 application rate in the second and third
cropping cycles although no deficiency symptoms were visual. Results from the study suggest
WTRs can be applied at rates up to 10 g kg-1 without reducing yield production. Heil and
Barbarick (1989) reported increased sorghum-sudangrass (Sorghum bicolor) growth above the
control when WTR was applied at 5 and 10 g kg-1. When application rates increased to 15 and
25 g kg- there was a 1.4 to 4.3 fold decline in plant yields.
Ippolito et al. (2002) studied the effects of combining WTR with biosolids on two range
grasses. Before testing it was assumed P availability would be reduced because of the chemical
composition of WTR and thus P was supplemented through the application of biosolids.
However, even when the two amendments were co-applied, P availability was reduced for blue
grama (Bouteloua gracilis) and western wheatgrass (Agropyron smithii) observed by the
lowering of tissue P. When WTR was applied alone, results showed increased Al uptake but no
effects on dry matter yield or P uptake.
Cox et al. (1997) performed a greenhouse study to determine the effects of alum sludge
application on P availability to wheat (Triticum aestivum L.) in a fine-loamy, Ultisol. Alum
sludge is a byproduct of the drinking water purification process using A12(S04)3 14 H20 and
Triple superphosphate was added in April and groundwater samples were not collected
until September. The rate of P movement between these time frames is unknown. The study
procedure represents a situation in which high loads of P would be applied after WTR
application. If the soil had been initially loaded with P throughout the upper soil profile,
groundwater data may have given different results especially visible in the incorporated
treatments.
Groundwater SRP concentrations were reduced by the surface application of WTR but
were not affected when the WTR was incorporated (Figure 4-12). The standard deviation of the
control surface treatment was very large in the first sampling but decreased considerably in the
second sampling while not having different SRP concentrations. The second sampling gave a
more stabilized and representative value of the SRP concentrations in these control treatments
and was still higher than treatments with WTR. There were no differences between the two
WTR application rates.
Table 4-9. The p-values for the groundwater SRP data using ANOVA and general mean contrast
(single degree of freedom).
Sampling Date Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
September 20, 2007 0.5805 0.0695 0.104 0.0301 0.504
October 2, 2007 0.8413 0.026 0.0305 0.0123 0.336
t Values italicized are significant at a = 0.05.
Figure 4-9 helps explain the differences in SPSC values of the incorporated and surface
applied treatments. The incorporated treatments had Al (from the WTR) mixed throughout the
top 10-15 cm of the soil profile by the tilling process. The A horizon soil samples were taken
above and below 8 cm. Samples were not mixed before being collected but rather taken at
regular increments along the length of the profile sampled (8 cm in this case) so the samples
from the surface applied treatments contained soil that did not include WTR. Recall that the soil
before WTR amendment had very low amounts of Fe and Al. Since this poor P retaining soil,
coupled with a larger amount of P from TSP application, was used to calculate the SPSC, the
value dropped below zero even though WTR was present. The soil sampling process involved
taking intact cores and placing them in sequence in a rain gutter. Subsequent subsampling from
each increment was done by compositing random samples taken with a spatula. This
subsampling protocol may have resulted in a non-uniform sampling of the given soil horizon
thereby explaining differences in SPSC between incorporated and surface applied treatments.
surface applied treatments. In general, less mature grasses have higher crude protein levels than
more mature grasses (Arthington and Brown, 2005). While values were lower in May, the less
mature grasses on the incorporated treatments almost doubled their crude protein content by
June, possibly explaining why values were higher than in the surface applied treatments.
Table 4-14. The p-values for the forage crude protein data using ANOVA and general mean
contrast (single degree of freedom).
Month Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
January 0.5604 0.4889 0.1951 0.4049 0.3948
May 0.0001 0.8384 0.9865 0.8705 0.5747
June <.0001 0.4749 0.6777 0.2871 0.5645
July 0.0058 0.982 0.5071 0.8514 1
August 0.0949 0.8471 0.7832 0.8514 0.5924
September 0.4913 0.1407 0.2947 0.0514 1
t Values italicized are significant at a = 0.05.
ft January data was collected before any amendments were applied. May through September
data was collected after WTR and TSP application.
Table 4-15. Crude protein percentage averages for incorporated and surface applied treatments.
Application Method January May June July August September
-------------------------------- Crude Protein %---------------------------------
Incorporated 6.10 a 8.28 b 15.25 a 12.42 a 10.19 a 13.19 a
Surface Applied 6.30 a 9.85 a 13.58 b 11.13 b 9.82 a 13.06 a
f Treatments with similar letters show no difference for that column by Waller-Duncan (p<0.05).
The crude protein percentage of bahiagrass grown in the summer ranged from 6.8 to
10.3%. Percentages dropped as maturity times increased from 4 to 6 weeks and 6 to 8 weeks
(Sollenberger et al., 2006). These values were lower than the bahiagrass values seen in our study
but may be indicative of an unfertilized system.
Tissue Nutrient Effects
Phosphorus
The pretreatment forage harvest showed no differences in P tissue concentration (0.12 -
0.15%) across all plots. Pretreatment concentrations were very low because of the initial low
soil P concentrations (See Table 4-1). Tissue P concentrations were affected by application
Hill, P.R. and J.V. Mannering. 1995. Conservation tillage and water quality. Purdue University
Cooperative Extension Service. http://www.ces.purdue.edu/extmedia/WQ/WQ-20.html.
Hsu, P.H. 1976. Comparison of iron III and aluminum in precipitation of phosphate from
solutions. Water Res. 10:903-907.
Hyde, J.E. and T.F. Morris. 2004. Phosphorus availability from phosphorus-amended water
treatment residual. Comm. in Soil Sci. and Plant Anal. 35:1033-1046.
Ippolito, J.A., K.A. Barbarick, and E.F. Redente. 2002. Combinations of water treatment
residuals and biosolids affect two range grasses. Commun. of Soil Sci. Plant Anal. 33:831-844.
Ippolito, J.A., K.A. Barbarick, D.M. Heil, J.P. Chandler, and E.F. Redente. 2003.
Phosphorus retention mechanisms of a water treatment residual. J. of Environ. Qual. 32:1857-
1864.
Jarvis, S.C. 1987. Laboratory studies of effects of lime on soluble aluminum, calcium,
magnesium and manganese in a pelostagnogley soil. J. of Soil Sci. 38:443-451.
Jenkinson, D.S. 1988. Determination of microbial biomass carbon and nitrogen in soil. p. 368-
386. In J.R. Wilson (ed.) Advances in nitrogen cycling in agricultural ecosystems. Oxford
University Press, Brisbane, Australia.
Kabata-Pendias, A. and H. Pendias. 2001. Trace Elements in Soils and Plants. 3rd ed. CRC
Press, Boca Raton, Fl.
Kalmbacker, R.S. and I.V. Ezenwa. 2006. Managing South Florida range for cattle. EDIS.
http://edis.ifas.ufl.edu/SS-AGRO105.
Kidder, G., C.G. Chamblis, and R. Mylavarapu. 2002. UF/IFAS standard fertilization
recommendations for agronomic crops. EDIS. http://edis.ifas.ufl.edu/SS401.
Kuo, S. 1996. Phosphorus. p. 869-919. In D.L. Sparks (ed.) Methods of soil analysis: Chemical
methods Part 3. ASA and SSSA, Madison, WI.
Li, Y.C., A.K. Alva, and D.V. Calvert. 1997. Transport of phosphorus and fractionation of
residual phosphorus in various horizons of a spodosol. Water, Air, and Soil Poll. 109: 303-312.
Lush, R.H. 1934. Calcium and phosphorus content of pasture grasses and legumes. J. of Anim.
Sci. 1934:254-257.
Makris, K.C., H. El-Shall, W.G. Harris, G.A. O'Connor, and T.A. Obreza. 2004.
Intraparticle phosphorus diffusion in a drinking water treatment residual at room temperature. J.
of Colloid and Interface Sci. 77: 417-423.
Table 4-17. Tissue P content. WTR application rate in Mg ha-1 is represented by 0, 35 and 70.
Application treatment is represented by I (incorporation) and S (surface application).
January May June July August September
Treatment ------------------------------Tissue Phosphorus %-----------------------------------
01 0.14 a 0.86 a 0.90 a 0.48 a 0.42 ab 0.43 a
35 0.13 a 0.57 b 0.63 b 0.36 ab 0.32 b 0.32 b
701 0.15 a 0.52 b 0.46 c 0.31 bc 0.26 c 0.29 bc
OS 0.14 a 0.89 a 0.71 b 0.50 a 0.49 a 0.46 a
35 S 0.12 a 0.37 c 0.33 cd 0.26 bc 0.22 0.24 cd
70 S 0.12 a 0.21 d 0.22 d 0.19 c 0.17 0.20 d
f Treatments with similar letters show no difference for that column by Waller-Duncan (p<0.05).
Tissue P concentrations were highest during May and June and then decreased to
relatively stable concentrations for the remainder of the harvests. The decreased P concentration
for July and August may be due to the higher yields during this time resulting in dilution of the
tissue P (Table 4-11). In addition, P concentrations in the soil solution may have decreased due
to leaching as 25 cm of rainfall occurred after TSP application (FAWN). The treatment most
impacted as measured by tissue P% was the surface applied 70 Mg ha-1 treatment. Tissue P
concentrations were low and relatively constant for this treatment throughout the entire study and
ranged from 0.17 to 0.22%. The August average of 0.17% was just slightly above the limiting
value of tissue P concentration of 0.15% (Mylavarapu et al., 2007).
Oladeji et al. (2006) observed decreasing plant P tissue concentrations with WTR
application rates at 1 and 2.5% dry weight by mass when fertilizers (biosolids, manures and TSP)
were applied at P agronomic rates of 44 kg P ha-1. However, when fertilizers were applied at N
agronomic rates (P application ranged from 88-280 kg P ha-1 depending on the N:P ratio in the
source), treatments amended with WTR had higher tissue P concentrations than the controls at P
agronomic rates. This suggests that under N agronomic rates, WTRs do not cause the tissue P to
drop below critical values even though tissue P is lowered. These results are similar to our study
where P was applied at high rates (224 kg P ha-1) to a WTR amended soil and tissue P levels
Al
Samples from
taken at WTR
8 cm
Incorporated
Treatments
Al
from
Samples WTR
taken at
8 cm
Surface Applied
Treatments
Figure 4-9. Differences in SPSC for incorporated and surface applied treatments.
Oladeji et al. (2006) also observed SPSC values in a bahiagrass field where WTR was
surface applied at rates up to 2.5% dry weight by mass. Various P sources were added at N and
P agronomic rates. It was found that independent of the P source or rate, SPSC increased as
WTR rates increased. The SPSC values in this study were calculated using oxalate extractable
concentrations. When P was applied with TSP at values greater than plant requirements which
were meant to mimic manure applications (88 kg P ha-1), the SPSC values were approximately
20 mg kg-1. In comparison, the SPSC values determined from Mehlich-1 concentrations in our
study showed values with an average of 6 mg kg-1 (3% dry weight WTR, surface applied and
under high TSP additions). Considering some differences existed between the two studies, SPSC
showed deeper incorporation of WTR increased P immobilization. Rew (2006) showed similar
results in a rainfall simulation involving the effects of an Al-WTR on manure impacted soils
with a fluctuating high water table. Boxes were filled with manure-impacted soils high in P and
either mixed with WTR or had WTR applied at the surface at a rate of 2.5% dry weight by mass.
Soluble P in runoff was more effectively reduced on the surface applied treatments while
incorporating the WTR was better at reducing soluble P in leachate and subsurface flow. Both of
these studies imply that incorporating the WTR allows for more contact with the impacted soil
and so better reduces downward movement of P than surface application. It was suggested that
to reduce P in runoff and leaching, WTR would need to be surface applied after being
incorporated (Rew, 2006).
Ippolito et al. (2003) reviewed the relationship between time and P desorption from a
WTR. Water treatment residual samples with a given load of P were shaken in a calcium
chloride solution for various lengths of time and analyzed to observe how much P was desorbed
from the amendment. After a linear decrease in the amount ofP sorbed (approximately 28 days),
the concentrations of P being desorbed quickly leveled off. The quick decline in P desorption
may represent P that was adsorbed to variable charged surfaces and the leveling off of desorption
may be a result of more strongly precipitated calcium phosphates.
The long term effects of P bound by WTR have not been extensively studied. In one
study by Agyin-Birikorang et al. (2007) the P bound by an Al-WTR was found to be stable after
7.5 years. A rainfall simulation demonstrated that WTR reduced dissolved and bio-available P
by more than 50% compared to controls. Therefore, it was shown that after 7.5 years the P
associated with WTR should not contaminate local waterways by runoff or leaching.
decreased but did not fall below the critical value. Rew (2006) observed no differences in shoot
P of stargrass when WTR was surface applied or mixed in the top 10 cm and 20 cm. Stargrass
was planted in boxes after the WTR and soil were mixed.
In a study by Codling et al. (2007) with WTRs, tissue P levels for corn grain and ear
leaves stayed above the minimum level needed for adequate growth (2 g kg-1) at WTR rates of 0,
5.6 and 11.2 Mg ha-1. Wheat showed deficient amounts of P (< 1.5 g kg-1) when WTR was
applied at rates of 2.5 and 5% dry weight by mass. The wheat in this study was grown in pots so
deficiencies may have been exacerbated because roots may have been constricted in the pots. In
the field, roots may have grown to depths below WTR application to obtain adequate P for plant
tissue.
Aluminum
Tissue Al for January, May and June showed concentrations below 370 [tg g-1 (data not
shown) which is within normal levels (50 and 3410 [tg g-l) as reported by Kabata-Pendias and
Pendias (2001). Concentrations did not increase with WTR application rate and no differences
occurred between treatments. Forage samples for July through September were analyzed with a
different instrument than the January, May and June samples because the original instrument
failed and could not be repaired in time to analyze the later samples. The substitute instrument
had a detection limit of 1.0 mg L-1, and the July through September samples had Al tissue
concentrations below this limit.
While Al tissue concentrations do not appear to have been affected by WTR application,
it is unusual for Al to be transported from the roots to the leaves so low Al concentrations in the
forage may not be indicative of signs of Al stress or toxicity. Cation uptake by roots is restricted
when Al toxicity is affecting plants. Thus, calcium (Ca) and magnesium (Mg) levels were also
evaluated.
To Beau Roberts, for being my mentor and challenging me in every facet of life;
and to Uncle Paul, for giving me a love and respect for agriculture.
Date
10
20
30
o 40
40
S50
C-
o 60
70
Figure 4-11. Water depth data from the pressure transducer. The ground surface is located at
zero on the y axis. The two sampling dates are circled and show they were collected
recently after rain events.
Many factors need to be considered when analyzing groundwater soluble reactive P
(SRP) concentrations. The water table from which the samples were collected was above the
spodic horizon. Nair et al. (1999) showed that lateral movement above the spodic horizon is
possible and it cannot be ruled out under the field conditions present in this study. Since SRP
concentrations did not change between the two sampling dates (Fig. 4-12) and there was a
difference due to WTR application in the surface applied treatments (Table 4-9), it is believed
that subsurface lateral flow did not cause substantial variations in groundwater SRP
concentrations. When the SRP concentrations were looked at on a plot map there were no trends
seen relating concentrations with subsurface movement. Sampling quickly after rain events may
have helped to prevent subsurface lateral flow effects from obstructing the results from WTR
application.
The main sources of P in Florida are fertilizers, animal manures, municipal biosolids and
effluents and composts. Application of manures and biosolids often occur at N agronomic rates
resulting in P levels which are higher than necessary (McCoy et al., 1986). Long histories of
high P applications have caused an accumulation of P to the point where many crops do not
benefit from added P. Although there is often little or no crop response from P fertilizers, many
farmers in Florida still apply higher amounts than necessary. In 1996, over 50% of P fertilizers
purchased in Florida were used in the South Florida Water Management District which is
especially susceptible to P loading in aquatic systems (Reddy et al., 1999).
Florida has more than 14 million hectares of acidic soils most of which are sandy (He et
al., 2005). These sandy soils are less capable of retaining P because unlike finer textured soils
they do not contain large quantities of mineral components such as metal oxides and secondary
phyllosilicates which aid in P retention (Sample et al., 1980). A study conducted on 96 Florida
sandy soils showed more than 10% of the soil P was water soluble suggesting high leaching
potential (Zhang et al., 2002). Harris et al. (1996) contend the main reasons for low P retention
in Alaquods are (i) very low amounts of aluminosilicate and metaloxide clays in the E horizon
and (ii) a seasonal shallow water table that results in the transport of P laterally into connected
drainage ways.
In 2007 Florida had 18,900 cattle farms and 440 dairy farms (USDA, 2008). Livestock
operations across the nation are intensifying their practices and creating Concentrated Animal
Feeding Operations (CAFOs). With this intensification comes more manure production per acre
of land. Before USEPA regulations, CAFOs applied manure at N agronomic rates resulting in
excessive P levels and possible P loss scenarios (USDA-USEPA, 1999). The risk of P loss can
be exacerbated with the poor sorbing soils found in Florida.
Phosphorus and Water Movement in Spodosols
There are approximately 4.5 million acres of Spodosols in Florida (Li et al., 1997).
Spodosols are characteristically nearly level, poorly drained, sandy soils with high seasonal
water tables and an organic Bh horizon. Naturally these soils are very infertile but can be highly
improved for crops or pasture if managed correctly (Soil Survey Staff, 1996).
Spodosols are formed from intensive acid leaching in moist areas. Acid organic
compounds bind Al or Fe and carry them down the soil profile by percolating waters. A
subsurface spodic horizon (Bh) forms when Al and Fe precipitate beneath an albic horizon. The
organic compounds make this horizon black in color (Brady and Weil, 2002). Spodosols can
cause a perched water table to exist. This can result in surface and subsurface runoff losses
because P does not come into contact with the Bh horizon where the Al and Fe have the
capability of forming precipitates and preventing P loss (Burgora, 1989).
Spodosols found in the Myakka series in Florida have an average water table depth of 76
cm (Allen, 1987). Nair et al. (1999) looked at how P retention was affected by the changing
aerobic and anaerobic conditions that occur with a fluctuating water table in Myakka soils. The
sandy upper A and E horizons allow P to move into the spodic horizon where it can become
trapped due to higher contents of Al and Fe. Anaerobic conditions led to equilibrium P
concentrations which were 30% greater than aerobic conditions and a P sorption maximum that
was less than 25% of aerobic conditions. These statements suggest P retention decreases when
the water table conditions are high (above the spodic horizon). Villapando and Graetz (2001)
similarly found that even though most of the P content of the Bh horizon was associated with Al
and Fe, a significant percentage was still in a highly mobile form which could be transported to
nearby waterways with lateral movement above the spodic. Conditions in which P could move
laterally above the Bh horizon depend on the P loading of the spodic: non-manure impacted soils
Nair, V.D. and W.G. Harris. 2004. A capacity factor as an alternative to soil test phosphorus in
phosphorus risk assessment. New Zealand J. of Ag. Res. 47:491-497.
Nannipieri, P., J. Ascher, M.T. Ceccherini, L. Landi, G. Pietramellara, and G. Renella.
1990. Microbial diversity and soil functions. European J. of Soil Sci. 54: 655-670.
Nichol, B.E., L.A. Oliveira, A.D. Glass, and M.Y. Siddiqi. 1993. The effects of aluminum on
the influx of calcium, potassium, ammonium nitrate, and phosphate in an aluminum-sensitive
cultivar of barley (Hordeum vulgare L.). Plant Phys. 101:1263-1266.
Novak, J.M., and D.W. Watts. 2004. Increasing the phosphorus sorption capacity of
southeastern coastal plain soils using water treatment residuals. Soil Sci. 169:206-214.
O'Connor, G.A., H.A. Elliott, and P. Lu. 2002. Characterizing water treatment residuals
phosphorus retention. Soil and Crop Sci. Soc. ofFl. Proc. 61:67-73.
O'Connor, G. A., 2005. Task # 6: Selection and Evaluation of Soil Amendments to Reduce
Edge-of-Field P Losses. IN "Demonstration of Water Quality Best Management Practices for
Beef Cattle Ranching in the Lake Okeechobee Watershed," Florida DEP Agreement No.: G0024
Oladeji, 0.0., G.A. O'Connor, J.B. Sartain, and V.D. Nair. 2006. Controlled application rate of
water treatment residual for agronomic and environmental benefits. PhD. Dissertation
University of Florida.
Parker, D.R., L.W. Zelazny, and T.B. Kinraide. 1989. Chemical speciation and plant toxicity of
aqueous aluminum. p. 117-146. In T.E. Lewis (ed.) Environmental chemistry and toxicology
of aluminum. C.R.C. Press, Upper Saddle River, NJ.
Paul, E.A., and F.E. Clark. 1996. Soil Microbiology and Biochemistry. Academic Press, San
Diego, CA.
Pecku, S., C.H. Hunter, and J.C. Hughes. 2006. The effects of water treatment residuals on soil
respiration and microbial community structure. Water Sci. and Tech. 54:215-225.
Pierzynski, G.M., R.W. McDowell, and J.T. Sims. 2005. Chemistry, cycling, and potential
movement of inorganic phosphorus in soils. p. 53-86. In J.T. Sims (ed.) Phosphorus:
Agriculture and the environment. ASA, CSSA, and SSSA, Madison, WI.
Powers, L.E. and R. McSorley. 2000. Ecological Principles of Agriculture. Delmar Thomson
Learning, Albany, NY.
Prosser, J.I. 2007. Microorganisms cycling soil nutrients and their diversity. pp 237-262. In
D. Elsas et al. (ed.) Modern soil microbiology, C.R.C. Press, Upper Saddle River, NJ.
2008 Julie Lauter Driscoll
S oil N itrification P otential............................................... .......................................... 59
Soluble Reactive Phosphorus in Groundwater............... .............. ...............61
Forage Y field and N utritive V alue................................................. .............................. 65
F o rag e Y field ................................................................6 5
N neutral D detergent Fiber ......... .... .............. ......... ........ .... ..... 67
C ru d e P ro te in ................................................................. ...... ..........................................6 8
Tissue N utrient Effects ....................................................... ................. 69
P h o sp h o ru s ............................................................................................................... 6 9
A lu m in u m ........................................................................................................... 7 2
Calcium and Magnesium....................................................................... 73
5 CONCLSUIONS ....................... ........ ..... .. ............. ........75
L IST O F R E F E R E N C E S ..................................................................................... ....................78
B IO G R A PH IC A L SK E T C H .............................................................................. .....................87
6
The soils on the site are from the Myakka soil series (sandy, siliceous, hyperthermic aeric
alaquod). This soil series is found extensively throughout Florida, covering more than 1.5
million acres. In Hardee County, it makes up more than 55,000 acres which is more than 13% of
the county's soils (Soil Survey Staff, 1996).
The study site had very low initial P concentrations (Mehlich-1 P = 2.1 mg kg-1 for the A
horizon). Additional P was surface applied in the form of triple superphosphate (TSP) with a
hand-held fertilizer spreader to all plots at a rate of 224 kg P ha-1. Applying TSP at high levels
allowed for the movement ofP throughout the soil profile to be evaluated. It also simulated
circumstances when P would be applied at high levels to the soil surface, such as in the
application of manures or biosolids at N agronomic rates. A timeline of the various activities on
the plots is shown in Table 3-1.
Table 4-8. The p-values for the nitrification potential data using ANOVA and general mean
contrast (single degree of freedom).
Sampling Date Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-
September 20, 2007 <.0001 0.0504 0.1386 0.1687 0.0369
t Values italicized are significant at a = 0.05.
20 a
-T
15 b
O
E c C
0-
Incorporated Incorporated Incorporated Surface Surface Surface
0 Mg / ha 35 Mg / ha 70 Mg / ha 0 Mg / ha 35 Mg / ha 70 Mg / ha
Application Method and Rate
Figure 4-10. Nitrification potential of treatments for soil samples taken on September 20, 2007.
f Treatments with similar letters show no significant difference by Waller-Duncan (p<0.05).
It is important to note that the values determined by this study are not actual field
nitrification rates but rather potential nitrification values. Factors which regulate nitrification
rates such as temperature, NH4+ supply and pH were controlled for optimal results.
In a study by Pecku et al. (2006), the effects of WTRs on soil respiration and microbial
community structure were evaluated. Six different WTRs were added to two soils at rates up to
500 Mg ha1. Soil microbial respiration increased as WTR application rate increased. This
increase was influenced not only by the application rate but also by the type of WTR and its
chemical characteristics. Respiration rates decreased with time (10 days) indicating WTR may
not enhance microbial activity for extended periods. Two WTRs had lower respiration rates
when combined with the soils. The lower rates were considered to be a result of the acidic
Soil pH is important when applying Al-WTRs to agricultural land because if the pH
drops below 4.0, Al becomes more soluble and toxicity implications become more serious. The
target pH for bahiagrass in Florida is 5.5 (Mylavarapu et al., 2007). The WTR used in this study
had positive effects on soil pH, showing an increasing trend with WTR application, but the soil
pH values even after WTR application suggest that liming of this soil should be recommended.
There may have been a slight acidifying effect from the ammonium nitrate fertilizer (89.7 kg N
ha-1) applied after each harvest. However all treatments, including the controls, received the
same fertilization so the relative effect of WTR application rate on soil pH can still be observed.
The pH of WTRs from different treatment plants can vary from 5.3 to 8.0 based on their
chemical makeup, how the WTR was produced and the characteristics of the water being
purified (Silveira et al., 2006, Codling et al., 2002, and Ippolito et al., 2003). Independent of the
exact pH of the material, WTRs have not expressed a strong effect on the soil pH in recent
studies. Ippolito et al. (2003) looked at changes in pH when WTR was shaken with a potassium
chloride solution. Subsamples were taken throughout a 215 day study and results showed the
initial pH of the WTR solution was 7.6 and increased to 8.15 after approximately 135 days. In
another study, soil samples from the top 2.5 cm with WTR applied at rates up to 18 Mg ha-1
showed no increase in pH after four months (Haustein et al., 2000). The pH of the WTR in this
study was 5.8 and the pH of the control plots was 5.5. In the same study, a HiClay Alumina
(HCA) amendment was compared to the WTR. The HCA had a pH of 3.4 and reduced the soil
pH from 5.53 (control) to 5.27 at an application rate of 18 Mg ha-l. Codling et al. (2007) applied
combinations of poultry litter and WTR at rates up to 11.2 Mg ha-l and observed minimal
changes in pH due to WTR application.
grown in a P impacted soil with WTR at rates of 2.5% dry weight by mass, no effects on yield
were observed (Rew, 2006).
In a study by Codling et al. (2007), corn grain yields were not adversely affected when
poultry litter was mixed with an Al-WTR at rates of 0, 5.6 and 11.2 Mg ha-1 and applied to plots
before corn was planted. However, wheat biomass yields were impacted at certain WTR rates in
a study by Codling et al. (2002). Three soils of various textures including a silt loam, fine sandy
loam and sand were mixed with WTR at rates of 0, 1, 2.5 and 5% dry weight by mass. Three
cycles of wheat were grown and analyzed in a greenhouse pot experiment. The fine sandy loam
and sand soils showed yield reductions at the 2.5 and 5% application rates but no effects were
found for the silt loam soil.
Neutral Detergent Fiber
Neutral detergent fiber (NDF) is often used as a forage quality indicator and measures the
amount of hemicellulose, cellulose and lignin in the forage. These polymers are not directly
composed of P, the element of concern for plant availability with WTR application. However,
observing no effect on NDF percentage does suggest that WTR application does not interfere
with reactions in the plant.
Table 4-12. The p-values for the forage NDF data using ANOVA and general mean contrast
(single degree of freedom).
Month Method Rate Method*Rate Control vs. 35 Mg ha- vs.
Treatments 70 Mg ha-1
January 0.9063 0.2741 0.1166 0.2185 0.2953
May 0.0553 0.6835 0.6908 0.4498 0.6787
June 0.0014 0.2438 0.6354 0.1529 0.3743
July 0.7035 0.9367 0.2993 0.8907 0.7426
August 0.0311 0.6239 0.8157 0.7243 0.3726
September 0.3016 0.7375 0.3985 0.7636 0.4787
t Values italicized are significant at a = 0.05.
ft January data was collected before any amendments were applied. May through September
data was collected after WTR and TSP application.
this depth, it did prevent the soil from becoming a P source as was observed in the negative
values of the controls.
Soil P Storage Capacity of Incorporated Treatments (mg kg1)
-40 -30 -20 -10 0 10 20 30 40 50 60 70
(0-8 cm)
A
(8-21 cm)
E
o Initial
* Control
[ 35 Mg / ha
S70 Mg / ha
Figure 4-7. Soil P storage capacity of incorporated treatments by soil horizon. The initial values
are the average SPSC values of all plots from the 2006 sampling before amendment
application. The control and WTR amended values are from the 2007 soil sampling
after amendment application.
Soil P Storage Capacity of Surface Applied Treatments (mg kg-1)
-40
A
(0-8 cm)
A
(8-21 cm)
E
t
0 10
I I
:2
o Initial
* Control
[ 35 Mg / ha
B70 Mg / ha
Figure 4-8. Soil P storage capacity of surface applied treatments by soil horizon. The initial
values are the average SPSC values of all plots from the 2006 sampling before
amendment application. The control and WTR amended values are from the 2007
soil sampling after amendment application.
I IE
kmmmmmmmmmmmmmm~
...............
CHAPTER TWO
LITERATURE REVIEW
Phosphorus
Phosphorus (P) is a macronutrient required by plants to provide and produce energy and
is the second most limiting nutrient. In most plants it comprises up to 0.2% of dry weight
(Schachtman et al., 1998), while legumes can have higher concentrations in the range of 0.4-
0.6% (Lush, 1934). It is essential to many cell components such as nucleic acids (DNA and
RNA), phospholipids and adenosine triphosphate (ATP) (Schachtman et al., 1998). ATP is
required for most processes within the plant that need energy including the uptake and transport
of nutrients. Phosphorus improves various plant physiological aspects such as nitrogen (N)
fixation, photosynthesis and root development. Phosphorus deficiency can be observed by
stunted, thin-stemmed and spindly plants. Some plants develop a purple color while extremely
deficient P levels may result in the yellowing of leaves. Deficiencies also cause delayed
maturity, sparse flowering and poor seed quality (Brady and Weil, 2002).
The movement of P in the environment is strongly affected by the form of P. Inorganic P
is normally found bound with aluminum (Al), iron (Fe), calcium (Ca) and magnesium (Mg)
(Graetz and Nair, 1999). Inorganic P is the most common form taken up by plants and is rarely
higher than 10 [iM in soil solution. The form of inorganic P changes according to pH. The
dissociation pKs of H3PO4 are 2.1 and 7.2 for H2P04- and HPO42-, respectively. Most P uptake
occurs in a pH range of 5.0-6.0 making H2P04- the most readily available form of inorganic P to
plants (Schachtman et al., 1998).
The Langmuir Equation is a mathematical model used to predict anion adsorption onto
reactive surfaces. This equation shows that the availability of phosphate is often controlled by
the amount of saturation at the sorption sites. As the sorption sites become saturated with anions
Table 4-20. Tissue Mg percentages for July through September.
Application Rate July August September
---------------------------Tissue Mg %-----------------------------
0 Mg ha1 0.32 a 0.35 a 0.28 a
35 Mg ha1 0.30 a 0.33 b 0.28 a
70 Mg ha-1 0.32 a 0.32 b 0.27 a
f Treatments with similar letters show no difference for that column by Waller-Duncan (p<0.05).
Codling et al. (2007) saw no changes in leaf or grain concentrations of Al, Ca or Mg at
WTR rates up to 16.8 Mg ha-1. Other minerals were also studied, including zinc, manganese,
iron and potassium. Similarly, there were no impacts from WTRs on these minerals. Rew
(2006) monitored stargrass tissue Al concentrations below 40 [tg g-1 in a rainfall simulation
experiment with WTR application rates up to 2.5% dry weight by mass. The Al concentration of
roots were also examined and showed no increases due to WTR. The Ca and Mg content in
forage averaged over flatwoods and fresh marsh sites in South Florida was 0.19% and 0.09%,
respectively (Kalmbacher and Ezenwa, 2006).
(0.05 N HC1 and 0.025 N H2SO4) was added. The tubes were shaken for five minutes and
immediately filtered through Whatman #41 filter paper. Samples were analyzed on a
TechniconTM Autoanalyzer.
The pH was determined by weighing 5 grams of soil and adding 10 mL DDI water giving
a 1:2 soil to solution ratio. The solution was stirred gently and after 30 minutes a reading was
taken with an Orion pH electrode.
Nitrification Potential
The nitrification potential of the soils was determined using the shaken soil slurry method
(Hart et al., 1996). The method was performed the day after soil collection. Fifteen grams of
field moist soil was added to a 250 mL Erlenmeyer flask. One hundred milliliters of a solution
containing 1.5 mM NH4 and 1.6 mM PO4-3 was added to each flask. The original procedure
called for 1.0 mM PO4-3 but this was increased to account for the low soil acidity. The buffer
solution was adjusted to pH 7.2 by adding NaOH dropwise. Parafilm with a slit cut on top was
placed securely over each flask to allow oxygen exchange but minimize evaporation. Samples
were placed on a shaker at 1,000 rpm in an incubator at 250C. Approximately 10 mL of slurry
was collected at 2, 4, 8, 20 and 24 hours and filtered through Whatman #40 filter paper. Samples
were stored in scintillation bottles and acidified with one drop of concentrated H2SO4. Analysis
for nitrate-N was performed on a rapid flow analyzer using EPA Method 353.2. The rate of
nitrate-N production (mg NO3-N L-1 h-) was calculated by linear regression of solution
concentration versus time. The gravimetric soil water content was determined for the same soil
samples (mass of water / mass of dry soil) and used to calculate the dry soil potential nitrification
rate.
Sodek, F., III., V.W. Carlisle, M.E. Collins, L.C. Hammond, and W.G. Harris. 1990.
Characterization data for selected Florida soils. Soil and Water Science Dep., Univ. of Florida,
Gainesville, FL. Res. 81-1.
Soil Survey Staff, Natural Resources Conservation Service, United States Department of
Agriculture. Soil Series Classification Database [Online WWW]. Available URL:
"http://soils.usda.gov/soils/technical/classification/scfile/index.html" [Accessed 20 June 2007].
Sollenberger, L.E., M.B. Adjei, W.F. Brown, K.H. Quesenberry, C.G. Chambliss, and W.E.
Kunkle. 2006. Floralta limpograss (Hemarthria altissima). EDIS. http://edis.ifas.ufl.edu/aa218.
Staples, C.R., R.S. Tervola, and E.C. French. 2003. Forage production practices by dairy
producers in the Suwannee Valley. Cooperative Extension Service, IFAS, University of Florida.
Gainesville, FL.
Strid, Hakan. 1996. Aluminum toxicity effects on growth and on uptake and distribution of
some mineral nutrients in two cultivars of spring wheat. Dissertation, Swedish University of
Agricultural Sciences.
Thompson, A., S.L. Hansard, and M.C. Bell. 1959. The influence of aluminum and zinc upon
the absorption and retention of calcium and phosphorus in lambs. J. of Anim. Sci. 18:187.
Torsvik, V., J. Goksoyr, and L. Daae. 1990. High diversity in DNA of soil bacteria. Applied
Environ. Microbiology 56:782-787.
Troeh, F.R., J.A. Hobbs, and R.L. Donahue. 2004. Soil and Water Conservation for
Productivity and Environmental Protection. Prentice Hall, Upper Saddle River, New Jersey.
Turco, R.F., A.C. Kennedy, and M.D. Jawson. 1994. Microbial indicators of soil quality. p. 73-
89. In Defining soil quality for a sustainable environment. SSSA, Minneapolis, MN.
USDA- Soil Conservation Service. 1984. Soil Survey of Hardee County Florida.
U.S. Department of Agriculture-U.S. Environmental Protection Agency (USDA-USEPA). 1999.
Unified National Strategy for Animal Feeding Operations. Available at
www.epa.gov/npdes/pubs/finafost.pdf.
USDA- National Agriculture Statistics Survey. 2008. http://www.nass.usda.gov/index.asp.
U.S. Environmental Protection Agency. 2000. National water quality inventory. EPA841-R-
00-001. USEPA, Office of Water, Washington, DC.
Vanderlip, R.L. 1993. How a sorghum plant develops. Kansas State University Agricultural
Experiment Station and Cooperative Extension Service.
http://www.oznet.ksu.edu/library/crpsl2/s3.pdf.
A number of best management practices (BMPs) have been designed to help control P
loss from agricultural soils. Fertilizer application rates, animal density, irrigation management,
runoff and wetland retention systems are being managed to help reduce eutrophication of aquatic
systems (Bottcher and Rhue, 1984). While these measures are helping to reduce P contamination
in Florida's aquatic systems, increasing populations and intensively managed agricultural
operations will place pressure to continually reduce the amount of P entering aquatic systems.
Controlling erosion, lowering levels of fertilizers in agricultural production and
implementing BMPs are approaches used to help prevent eutrophication (Troeh et al., 2004).
Several physical means of reducing surface runoff to minimize P losses are currently in practice:
conservation tillage, buffer strips, runoff water impoundments and riparian zones (Novak and
Watts, 2004). Recently, a variety of chemical soil amendments have been suggested as means of
increasing P retention by the soil and thereby reducing P losses.
Aluminum (Al) and iron (Fe) salts (A12(S04)3, FeC13) have been shown to lower the
amounts of labile P in the soil but often have high costs which detract from their potential
widespread use (Moore and Miller, 1994). Water treatment residuals (WTRs) are by-products of
the drinking water purification process that contain amorphous Fe or Al oxides or calcium
carbonate as well as sediment and humic substances from the treated water (Makris et al., 2004).
The Fe and Al compounds in WTRs contain reactive hydrous oxides with substantial P-fixing
capabilities (Elliott et al., 2002). The current methods of WTR disposal are expensive for water
treatment municipalities and include storage in landfills and onsite lagoons and discharge into
sanitary sewer systems (Dayton and Basta, 2001). Thus, other methods of disposal and
utilization such as land application are being evaluated. Ippolito et al. (2003) list some of the
possible benefits of WTR land application: increased N and organic carbon availability for
leaching. Mehlich-1 Al increased from 22 mg kg-1 to 390 mg kg-1 (3% WTR rate) in the A
horizon, giving the soil a greater P retention capacity which was observed in the increasing soil P
storage capacity (SPSC) values which occurred with increasing WTR rate as well. The SPSC
values increased on plots with WTR even after heavy loads of P were applied and some
remained high enough that additional P should be retained. Potential nitrification rates increased
with higher WTR application rates, indicating no adverse effects occurred on soil microbial
populations because of WTR application. Groundwater SRP concentrations decreased with the
addition of WTR by 78% in the surface applied treatments. There were no differences in SRP
concentrations among the incorporated treatments which was likely a temporary result of the
tilling process. Forage yield, crude protein and neutral detergent fiber were not affected by WTR
application. Tissue Al did not increase and tissue calcium and magnesium uptake was not
restricted. Tissue P decreased as WTR application rate increased. Tissue P levels were higher in
the incorporated treatments than the surface applied treatments. However, tissue levels did not
fall below the bahiagrass limiting value (0.15%).
Based on this study, the use of WTRs is recommended as an amendment to soils with low
P retention capacities that have been heavily impacted with P or will receive high amounts of P
e.g., a dairy sprayfield.
Aluminum toxicity is one of the major problems and growth limiting agents in acidic
soils. Toxicity occurs when the pH falls below 5.5 and becomes increasingly damaging below
5.0 (Foy et al., 1978). The trivalent form of Al (Al+3) dominates solutions when the pH is below
5.0; Al(OH)+2 and AI(OH)2 become more prevalent as pH increases. The trivalent form is
assumed to be the phytotoxic form of Al because other trivalent cations are also toxic to plants
and because toxicity is associated with acidic soil conditions (pH less than 5.0) (Delhaize and
Ryan, 1995). Taking into consideration that WTRs are often buffered at 7.0 and agricultural
fields are often maintained above pH 5.5 for optimal growing conditions, Codling et al. (2007)
contend Al toxicity should not issue problems since Al+3 solubility decreases as pH approaches
5.2. Soils high in organic carbon content may form Al complexations further reducing toxicity
possibilities (Hsu, 1976).
Acidification increases Al and manganese solubility, thereby decreasing root growth
(Jarvis, 1987). The process of soil acidification is mainly caused by heavy rains which strip
major cations from the soil. It can also result from using acidifying fertilizers (Mora et al.,
1999).
Aluminum toxicity causes less P uptake into the plant and also disables its movement
within the roots (Edwards, 1991). Damage due to Al toxicity arises from decreased nutrient
uptake and transport of other vital nutrients, especially Ca, potassium and Mg, as well as from
imbalanced cation to anion ratios (Kabata-Pendias, 2001). Nichol et al. (1993) observed the
effects on cation uptake at Al levels of 100lM. Calcium uptake was inhibited by 69%,
ammonium by 40% and potassium by 13%. Possible mechanisms of reducing cation uptake
include the Al+3 ions blocking or binding to negative charges in proteins of the plasma
membrane. Even in micromolar concentrations, Al can cause stress in plants observable by the
Makris, K.C., G.A. O'Connor, W.G. Harris, and T.A. Obreza. 2005. Relative efficacy of a
drinking-water treatment residual and alum in reducing phosphorus release from poultry litter.
Comm. in Soil Sci. and Plant Anal. 36:2657-2675.
McBride, Murray B. 1994. Environmental Chemistry of Soils. Oxford University Press,
New York, NY.
McCoy, J.L., L.T. Sikora, and R.R. Weil. 1986. Plant availability of phosphorus in sewage
sludge compost. J. of Environ. Qual. 15:405-409.
McKeague, J.A., J.E. Brydon, and N.M. Miles. 1971. Differentiation of forms of extractable
iron and aluminum in soils. Soil Sci. Soc. of Am. Proc. 35:33-38.
Mehlich, A. 1953. Determination of P, Ca, Mg, K, Na, and NH4. p. 1-53. In Soil testing
division publications. North Carolina Department of Agriculture, Raleigh, NC.
Miyittah-Kporgbe, M. 2004. Phosphorus immobilization in manure-impacted soil with
aluminum-based drinking water treatment residual. M.Sci. Thesis, Univ. of FL, Gainesville, Fl.
Mookherji, S. and M. Floyd. 1991. The effect of aluminum on growth of and nitrogen fixation
in vegetable soybean germplasm. Plant Soil 136:25-29.
Moore, P.A..Jr., T.C. Daniel, and D.R. Edwards. 2000. Reducing phosphorus runoff and
inhibiting ammonia loss from poultry manure with aluminum sulfate. J. of Environ. Qual. 29:37-
49.
Moore, P.A.Jr,. and D.M. Miller. 1994. Decreasing phosphorus solubility in poultry litter with
aluminum, calcium, and iron amendments. J. of Environ. Qual. 23:325-330.
Mora, M.L., B. Schnettler, and R. Demanet. 1999. Effect of liming and gypsum on soil
chemistry, yield and mineral composition of ryegrass grown in an acidic andisol. Comm. in Soil
Sci. and Plant Anal. 30:1251-1266.
Mylavarapu, R., D. Wright, G. Kidder, and C.G. Chambliss. 2007. UF/IFAS standardized
fertilization recommendations for agronomic crops. EDIS. http://edis.ifas.ufl.edu/ssl63.
Myrold, D.D. 1999. Transformations of nitrogen. p. 259-294. In D.M. Sylvia et al. (ed.)
Principles and applications of soil microbiology. Prentice Hall, Upper Saddle River, NJ.
Nair, V.D. and D.A. Graetz. 2002. Phosphorus saturation in Spodosols impacted by manure. J.
of Environ. Qual. 31:1279-1285.
Nair, V.D., R.R. Villapando, and D.A. Graetz. 1999. Phosphorus retention capacity of the
spodic horizon under varying environmental conditions. J. of Environ. Qual. 28:1308-1313.
produced yearly (about 4,000 Mg yr-1). The recommended rate of application was 2.5% dry
weight by mass (O'Connor, 2005); the rates of application for this study were 1.5 and 3.0% dry
weight by mass.
The initial size of the WTR particles varied largely from clay size upwards to 12 cm so
they were passed through a 0.64 cm mesh to obtain better uniformity. The moisture content of
the WTR was not high enough to cause a problem when sieving and so it was not previously air
dried. Sieved WTR was stored at the study site under visqueen tarps until application. Five
random samples were taken the day prior to WTR application to determine the average moisture
content of the Al-WTR. Given the 30.3% moisture content, the amount of WTR needed for the
35 and 70 Mg ha-1 application rates was 93 and 186 kg per plot, respectively. The WTR was
applied with a drop fertilizer spreader to assure an even distribution.
Soil Sampling
Two soil samplings were performed during the course of the study. An initial sampling
before any treatment was applied was taken in August 2006 and the second sampling was taken
in August 2007. Two holes were augured to a depth of one meter for each plot. The profile was
carefully laid out in rain gutters and depths noted at each change in horizon (Table 3-2). One
composite sample from each horizon was then taken from both profiles. The A horizon was split
into two depth increments, one from the top 8 cm and one from below 8 cm. This technique was
followed to better quantify differences due to the incorporation process. All soil samples were
placed in plastic bags and kept on ice until they were transported to Gainesville, FL, for
laboratory analysis. All samples were air-dried before analysis.
the concentration of the anion in solution increases. Desorption of phosphate creates a condition
of hysteresis in which desorption is much slower than adsorption to such an extent that it does
not affect soil processes. Hysteresis has important environmental implications because it allows
the soil to hold onto phosphate it has sorbed even once its concentration decreases. This
ultimately results in less leaching; however, it is only effective when soils have the ability to sorb
phosphate or when the soil has not been oversaturated (McBride, 1994).
Soils can become loaded with high concentrations of P through agricultural management
practices. Manure from local livestock productions is often applied as a fertilizer based on plant
N requirements which results in P application rates that exceed plant needs (Sharpley et al.,
1994). Excessive fertilizer usage and biosolids application can also load soils with excessive P
(O'Connor et al., 2002). Graetz and Nair (1999) reported that up to 80% of total P has the
potential to be transported in heavily manure-impacted upland soils. Repeated additions of P
over time reduce the soil's capacity to retain P (Reddy et al., 1999).
Soils in the eastern Coastal Plain are characteristically highly weathered and coarse
textured soils which have a lowered affinity to sorb P. When these soils with low P retention
capacity are coupled with high loads of P, the ability to bind P is significantly lowered (Novak
and Watts, 2004). Florida has many low P sorbing soils and hydraulic conditions that allow for
P to easily leach into bodies of water causing environmental problems (O'Connor et al., 2002).
Soil test P (STP) and degree of P saturation (DPS) are indicators often used to assess
environmental risks associated with P application. Neither indicator however accounts for the
soil's capacity to retain P. Although STP and DPS values may be low, applying high amounts of
additional P could result in excess P not bound by the soil and induce risks to local water
systems. Nair and Harris (2004) proposed using a calculation to determine the soil P storage
available P. Leaf Al concentrations were not affected by WTR and did not reach toxic levels
(Codling et al., 2007). A similar study was conducted by Codling et al. (2002) on wheat grown
in a greenhouse where P deficiencies were observed when the WTR application rate was 25 and
50 g kg-1. The differences in P uptake between greenhouse and field conditions were attributed
to the confining volume of growing pots. Under greenhouse potting conditions, the volume of
soil to supply P is limited (Codling et al., 2007).
A study by Oladeji et al. (2006) evaluated the effects of an Al-WTR on bahiagrass
(Paspalum notatum) in a glasshouse study. The WTR was applied at three rates (0, 10, and 25 g
kg-1) on P deficient soils in combination with various P sources applied at N and P agronomic
rates. Bahiagrass tissue P concentrations fell between 1.5 to 6.0 g kg-1, right around the critical P
value of approximately 2.0 g kg-1 used in this study. Dry matter yields were not affected by
WTR application. From this study it was concluded that in order to avoid agronomic
deficiencies, WTR application rates should be based upon the Al, Fe and P composition of the
soil, WTR, and P-source. Applying WTRs so soil P storage capacity is at zero allows for P
uptake in plants to be minimally impacted and increased environmental benefits such as less P
leaching.
Aluminum
Aluminum makes up 7% of the earth's crust by mass and is the most abundant element in
the crust (Delhaize and Ryan, 1995). It is found in all plants and some more tolerant species may
contain more than 0.1% of Al by dry weight. Tissue samples from grass tops contained 50-3410
pg Al g-1 by dry weight (Kabata-Pendias and Pendias, 2001). Although the physiological
function of Al in plants is not clearly known it may be beneficial to some plants in small
quantities in its ability to activate some enzymes and control plasma and membrane permeability.
There was considerable movement of WEP downward in the soil profile in the control
treatments four months after TSP application. Increases in WEP concentrations were observed
in all horizons except the B horizon (Figs. 4-1 and 4-2). It is not possible to determine if the
lack of response in the B horizon was due to the P bonding strength of the B horizon or to the
lack of movement to this depth. Application of WTR decreased the WEP concentrations relative
to the control in all horizons except the B horizon where there was no effect. Differences
between the 35 and 70 Mg ha-1 WTR application rates were only significant in the A (0-8 cm)
horizon (Table 4-5).
The WEP concentrations in the incorporated treatments of the A (8-21 cm) and E
horizons were greater than the surface applied treatments (Table 4-5) suggesting there may be a
potential effect of tillage on P transport in the soil. Tilling destroys macropores which allow for
more rapid water movement (Hill and Mannering, 1995). Thus, no-tillage systems have greater
water movement which may result in faster P transport and may help explain the WEP
concentrations between the incorporated and surface applied treatments. This P may not be
observable in the B horizon due to extensive bonding by Al in the spodic horizon.
These results are similar to previous results reported in the literature. Codling et al.
(2000) studied the effects of WTRs and iron rich residues (IRR) when added to poultry litter and
soils. Each amendment was added to the litter and soils at rates up to 10% dry weight by mass.
Samples were incubated for 7 weeks and subsamples taken at 2, 4 and 7 weeks. The litter and
WTR combination showed a decrease in water soluble P (WSP) as WTR rate increased. The
WTR reduced WSP more than the IRR. Reductions in WSP compared to the controls at the
highest application rate were 87% and 42% for the fourth week for WTR and IRR, respectively.
Similar results were shown when a WTR was added to three different soils at rates up to 5% dry
Sikora (2004) investigated the effects of Al-WTR on plant residue decomposition. The
WTR was applied to two Ultisols at rates of 0.5, 2.5 and 10 g kg-1. Carbon labeled soybean
leaves and stems were added. Lime (CaO) was also added to evaluate the effects of raising the
pH. There was no effect on soybean residue decomposition by WTR or CaO addition. Salinity
increases were negligible and did not affect the other soil characteristics studied. It was
recommended that the WTR application rate should have the same metal molar concentration as
soil P molar concentration.
Bahiagrass
Bahiagrass is a warm season perennial common throughout Florida. It can sustain high
temperatures and low periods of rain which often limit the growth of other grasses. In Florida,
bahiagrass covers approximately 2.5 million acres making it the highest single used grass pasture
species (Chambliss and Adjei, 2006). It is e used extensively in Florida because of its ability to
tolerate a wide range of soil conditions, produce moderate yields even when the soil has low
fertility, establish easily from seed, withstand close grazing and is not regularly affected by
insects and disease. Mylavarapu et al. (2007) determined the limiting value of bahiagrass to be
1.5 g kg- The limiting value is defined as the tissue concentration above which added P will
have no effect on plant yield.
Aluminum has no known biological value to animal nutrition, thus it is almost never
added to the diet. It can be ingested through soil or grass. In some animals, Al at certain levels
can cause P deficiencies. Toxicity is altered by the solubility of Al and quantity of P in the diet.
Thompson et al. (1959) proposed ruminant P levels were not as negatively impacted by the
consumption of excess Al. Based on results from numerous studies, the Board on Agriculture
(1980) set the maximum tolerable level for Al for sheep and cows at 1,000 ppm.
Since bahiagrass is such an important and widely used forage in Florida cattle production,
it is vital to consider the impact WTRs may have on forage yield and tissue nutrient
concentrations, namely Al. Phosphorus levels should not drop below levels which would
decrease yields. Aluminum can affect animal dietary needs and should also be monitored, not
only in the amount consumed in the forage but also in the soil.
Reddy, K.R., E. Lowe, and T. Fontaine. 1999. Phosphorus in Florida's ecosystems: analysis of
current issues. p. 111-142. In K.R. Reddy et al. (ed.) Phosphorus biogeochemistry in
subtropical ecosystems. Lewis Publishers, Boca Raton, FL.
Rew, T.J., Graetz, D.A., Nair, V.D., O'Connor, G.A., and Sollenberger, L.E. 2006. Aluminum
water treatment residuals for reducing phosphorus loss from manure-impacted, high-watertable
soils. M. of Sci. Univ. ofFL, Gainesville, FL.
Sample, E.C., R.J. Soper, and G.J. Racz. 1980. Reactions of phosphate fertilizers in soils. p. 263-
310. In F.E. Khasawneh et al. (ed.) The role of phosphorus in agriculture. ASA, CSSA, and
SSSA, Madison, WI.
SAS Institute, 2001. The SAS System for Windows. Version 8 Release 8.2 SAS Inst. Cary, NC.
Schachtman, D.P., R.J. Reid, S.M. and Syling. 1998. Phosphorus uptake by plants: from soil to
cell. Plant Phys. 116:447-453.
Schertz, D.L. 1994. Conservation tillage- A national perspective. p. 1-5. In P.J. Bauer and
W.J. Busscher (ed.) Proceedings of the 1994 southern conservation tillage conference for
sustainable agriculture. USDA-ARS, Florence, S.C.
Schumann, A.W. and R. MunozCarpena. 2002. A simple, selfcontained canal stage recorder.
Applied Eng. in Ag. 18(6): 691-696.
Sharpley, A.N., S.C. Chapra, R. Wedepohl, J.T. Sims, T.C. Daniel, and K.R. Reddy. 1994.
Managing agricultural phosphorus for the protection of surface waters: Issues and options. J. of
Environ. Qual. 26:437-451.
Sharpley, A.N., R.W. McDowell, and P.J. KleinmanJ. 2001. Phosphorus loss from land to
water: integrating agricultural and environmental management. Plant Soil 237:287-307.
Shipitalo, M.J., W.A. Dick, and W.M. Edwards. 2000. Conservation tillage and macropore
factors that affect water movement and the fate of chemicals. Soil and Tillage Res. 53:167-183.
Shreve, B.R., P.A. Moore, T.C. Daniel, D.R. Edwards, and D.M. Miller. 1995. Reduction of
phosphorus in runoff from field-applied poultry litter using chemical amendments. J. of Environ.
Qual. 24:106-111.
Sikora, L.J. 2004. Effect of industrial byproducts containing high aluminum or iron levels on
plant residue decomposition. Comm. in Soil Sci. and Plant Anal. 35:921-936.
Silveira, M.L., M.K. Miyittah, and G.A. O'Connor. 2006. Phosphorus release from a manure-
impacted spodosol: effects of a water treatment residual. J. of Environ. Qual. 35:529-541.
suggesting even more P can be retained if added to the soil. Incorporation of the WTR is
recommended based on two main factors: bahiagrass tissue P concentration was less affected
under these conditions and the SPSC benefited greater in the A horizon. However, the physical
incorporation process is not always practical. If applying WTR to a large area, surface
application may be easier and less destructive when considering extra time is needed for
regrowth after disking. Surface application is still very effective at P retention, especially when
the P source is being applied after WTR application. No adverse effects were observed by
forage yield or nutritive value at any rate. Therefore, the 70 Mg ha-1 application rate is
recommended since it adds a greater amount of Al to the soil, further increasing the capacity of
the soil to retain P than lower application rates. Forage yield and tissue P concentrations need to
be analyzed in the future to assure toxicities do not develop with time.
The prevention of P loss from agricultural fields is essential for the health of aquatic
systems. The application of Al-WTRs has the potential to achieve these goals but the quality of
the crop must also be seriously regarded. Different crops may have varying sensitivities to Al in
the soil or to how much tissue P is required for normal plant growth and function. Application
method and rate are important factors to consider when applying WTRs. Future analysis of the
soil and forage on this study site would be useful to assess long term implications of WTR usage.
- -- Initial -- Control
--35 Mg / ha
(0-8 cm)
A
(8-21 cm)
E
--70 Mg/ ha
Mehlich-1 P of Incorporated Treatments (mg kg-1)
Figure 4-3. Mehlich-1 P concentrations of incorporated treatments by soil horizon. The dashed
line represents the average concentration of all plots from the 2006 sampling before
amendment application. The other lines represent the WEP concentrations from the
2007 soil sampling after amendment application.
--*--Initial
--35 Mg / ha
- Control
-70 Mg / ha
(0-8 cm) P
Soil Horizon i
A i
(8-21 cm)
E -
Mehlich-1 P of Surface Applied Treatments (mg kg)
Figure 4-4. Mehlich-1 P concentrations of surface applied treatments by soil horizon. The
dashed line represents the average concentration of all plots from the 2006 sampling
before amendment application. The other lines represent the WEP concentrations
from the 2007 soil sampling after amendment application.
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ALUMINUM WATER TREATMENT RESIDUAL EFFECTS ON SOIL PHOSPHORUS RETENTION AND FORAGE YIELD AND NUTRITIVE VALUE By JULIE LAUTER DRISCOLL A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLOR IDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2008 1
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2008 Julie Lauter Driscoll 2
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To Beau Roberts, for being my mentor a nd challenging me in every facet of life; and to Uncle Paul, for giving me a love and respect for agriculture. 3
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ACKNOWLEDGMENTS I would first like to thank Dr. Don Graetz for all of his support in bo th the work on this thesis and with the Agronomy and Soils Club. I also thank my committee members Dr. Dean Rhue and Dr. Maria Silveira for all of their time and effort in helping me compile my work. I would like to pay my respects to Dr. Martin Adjei who passed away shortly after this study began. I also thank the Soil and Water Science De partment for their input and guidance in every aspect of my work, especially Dr. Nair Dr. Ogram, Dr. Harris and Mace Bauer. I extend my deepest thanks to Dawn Lucas for being my mom away from home and helping with all of my laborator y analysis. I would also like to thank everyone in Lab 212 for their advice, help with lab work and overall friendships: Myrlene, Lacey, Ryan, Brandon, Leighton and Dan. I thank Richard Fethiere and the Forage Evaluation S upport Lab for allowing me to do so much of my forage work in your lab. Thank you to Bill Reves and Nancy Wilkinson for help in analyzing samples. I thank Cindy Ho lley for all her efforts a nd help in coordinating field work in Ona. Also, thanks go to all w ho helped work in field during those hot summer months: TJ, Kenny, David and especially my Dad. I would like to thank my family for all of th eir support and love throughout the years. Ill finally be the Dr. of Dirt as you like to refer to me. Thanks also go to the Agronomy and Soils Club advisors and members for teaching me to be a leader and for all of the lifelong friendships. Finally, I would like to thank all my friends for two great years of graduate school. Hughes, thanks for all the fun with biochem and sampling months; Miller, thanks for being my best friend in the department and willingly participating in so many Gator events; Bri and Tom, thanks for taking on regionals and the Appa lachian Trail with me. 4
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TABLE OF CONTENTS page ACKNOWLEDGMENTS...............................................................................................................4 LIST OF TABLES................................................................................................................. ..........7 LIST OF FIGURES.........................................................................................................................9 ABSTRACT...................................................................................................................................10 CHAPTER 1 INTRODUCTION................................................................................................................. .12 2 LITERATURE REVIEW.......................................................................................................16 Phosphorus..............................................................................................................................16 Phosphorus Retention by WTR..............................................................................................18 WTR-Induced P Deficiency in Plants.....................................................................................22 Alumin um....................................................................................................................... ........24 Phosphorus and Water Movement in Spodosols....................................................................27 Soil Microorganisms............................................................................................................ ...28 Bahiagrass..................................................................................................................... ..........30 3 MATERIALS AND METHODS...........................................................................................32 Site Description and Experimental Design.............................................................................32 Water Treatment Residual......................................................................................................3 4 Soil Sampling.................................................................................................................. ........35 Groundwater Sampling........................................................................................................... 36 Analytical Methods............................................................................................................. ....37 Soil...................................................................................................................................37 Nitrification Potential......................................................................................................38 Groundwater....................................................................................................................39 Forage..............................................................................................................................39 Quality Assurance and Quality Control..................................................................................40 Statistical Analysis........................................................................................................... .......40 4 RESULTS AND DISCUSSION.............................................................................................41 Soil Properties.........................................................................................................................41 Initial Soil Characteristics...............................................................................................41 Aluminum Water Treatment Residual Characteristics....................................................42 Water Extractable Phosphorus........................................................................................45 Mehlich-1 Extractions.....................................................................................................49 Soil Phosphorus Storage Capacity...................................................................................54 5
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Soil Nitrification Potential...............................................................................................59 Soluble Reactive Phosphorus in Groundwater................................................................61 Forage Yield and Nutritive Value...........................................................................................65 Forage Yield....................................................................................................................65 Neutral Detergent Fiber...................................................................................................67 Crude Protein...................................................................................................................68 Tissue Nutrient Effects....................................................................................................69 Phosphorus...............................................................................................................69 Alumin um.................................................................................................................72 Calcium and Magnesium..........................................................................................73 5 CONCLSUIONS.................................................................................................................. ..75 LIST OF REFERENCES...............................................................................................................78 BIOGRAPHICAL SKETCH.........................................................................................................87 6
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LIST OF TABLES Table page 3-1 Dates and descriptions of field activities...........................................................................34 3-2 Depths to the bottom of the soil horiz ons sampled. The A horizon was sampled from 0-8 cm and 8 cm to the bottom of the horizon...................................................................36 4-1 General characteristics in soil samples taken prior to amendment application (August 2006) averaged for all plots...............................................................................................42 4-2 General characteristics of the Manatee County Al-WTR..................................................43 4-3 The p-values for the pH data usi ng ANOVA and general mean contrast (single degree of freedom).............................................................................................................43 4-4 Soil pH averages for incorporated an d surface applied treatm ents 8 months after WTR application................................................................................................................43 4-5 The p-values for the WEP data usin g ANOVA and general mean contrast (single degree of freedom).............................................................................................................45 4-6 The p-values for the Mehlich-1 data using ANOVA and general mean contrast (single degree of freedom).................................................................................................49 4-7 The p-values for the SPSC data usin g ANOVA and general mean contrast (single degree of freedom).............................................................................................................55 4-8 The p-values for the nitrification potential data using ANOVA and general mean contrast (single degree of freedom)...................................................................................60 4-9 The p-values for the groundwater SRP data using ANOVA and general mean contrast (single degree of freedom)...................................................................................63 4-10 The p-values for the forage yield data using ANOVA and general mean contrast (single degree of freedom).................................................................................................66 4-11 Forage yield averages for incorporated and surface applied treatments............................66 4-12 The p-values for the forage NDF da ta using ANOVA and general mean contrast (single degree of freedom).................................................................................................67 4-13 Forage NDF percentage averages for incorporated and surface applied treatments.........68 4-14 The p-values for the forage crude protein data using ANOVA and general mean contrast (single degree of freedom)...................................................................................69 4-15 Crude protein percentage averages for in corporated and surface applied treatments.......69 7
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4-16 The p-values for the forage tissue P data using ANOVA and general mean contrast (single degree of freedom).................................................................................................70 4-17 Tissue P content......................................................................................................... ........71 4-18 The p-values for the forage tissue Ca and Mg data using ANOVA and general mean contrast (single degree of freedom)...................................................................................73 4-19 Tissue Ca percentages for July through September...........................................................73 4-20 Tissue Mg percentages for July through September..........................................................74 8
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LIST OF FIGURES Figure page 3-1 Plot design and layout..................................................................................................... ...32 4-1 The WEP concentrations of incorporated treatments by soil horizon...............................47 4-2 The WEP concentrations of surf ace applied treatments by soil horizon...........................47 4-3 Mehlich-1 P concentrations of in corporated treatments by soil horizon...........................51 4-4 Mehlich-1 P concentrations of surface applied treatments by soil horizon.......................51 4-5 Mehlich-1 Al concentrations of incorporated treatments by soil horizon.........................53 4-6 Mehlich-1 Al concentrations of surface applied treatments by soil horizon.....................53 4-7 Soil P storage capacity of incor porated treatments by soil horizon...................................56 4-8 Soil P storage capacity of surface applied treatments by soil horizon...............................56 4-9 Differences in SPSC for incorporat ed and surface applied treatments..............................58 4-10 Nitrification potential of treatments for soil samples taken on September 20, 2007.........60 4-11 Water depth data from the pressure transducer..................................................................62 4-12 Water SRP concentrations across trea tments for two sampling dates in 2007..................64 9
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Abstract of Thesis Presen ted to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Master of Science ALUMINUM WATER TREATMENT RESIDUAL EFFECTS ON SOIL PHOSPHORUS RETENTION AND FORAGE YIELD AND NUTRITIVE VALUE By Julie Lauter Driscoll May 2008 Chair: Donald Graetz Major: Department: Soil and Water Science The health of aquatic systems is being threatened by the addition of phosphorus (P) which causes increased eutrophication. Many Flor ida soils have naturally low capacities to retain P, allowing for P loss to local waterways by leaching. In addition, P is often over-applied when animal manures are used as fertilizers, which further increases the chances of P loss. Water treatment residuals (WTRs) are by-produc ts of the water purif ication process which contain high amounts of amorphous aluminum (Al) or iron, giving them the cap acity to retain P. Land application of WTRs has been investigated mainly in greenhouse experiments to test the P sorbing capacities of WTRs and to evalua te potential Al toxi city to plants. The purpose of this project was to evaluate the effects of an Al-W TR on soil P retention, soil microbial population, groundwater soluble reactive P (SRP) con centrations and forage yield and nutritive value. Three WTR application ra tes (0, 1.5 and 3% dry weight by mass) and two application methods (soil incorporation and surface applied) were tested on a bahiagrass ( Paspalum notatum ) pasture in Ona, FL. Soils on the site had low P levels (A horizon = 2.1 mg Mehlich-1 P kg-1) so triple superphospha te was added at 224 kg ha-1 to simulate high P loadings. The addition of WTR showed a trend toward slightly high er pH. Water extractable P concentrations decreased as WTR application ra te increased, suggesting less P will be lost to 10
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leaching. Mehlich-1 Al increased from 22 mg kg-1 to 390 mg kg-1 (3% WTR rate) in the A horizon, giving the soil a greater P retention capacity which was obs erved in the increasing soil P storage capacity (SPSC) values which occurred with increasing WTR rate as well. The SPSC values increased on plots with WTR even afte r heavy loads of P were applied and some remained high enough that additional P should be reta ined. Potential nitrification rates increased with higher WTR application ra tes, indicating no adverse e ffects occurred on soil microbial populations because of WTR application. Groundw ater SRP concentrations decreased with the addition of WTR by 78% in the surface applied tr eatments. There were no differences in SRP concentrations among the incorporated treatmen ts which was likely a temporary result of the tilling process. Forage yield, cr ude protein and neutral detergent fiber were not affected by WTR application. Tissue Al did not increase a nd tissue calcium and magnesium uptake was not restricted. Tissue P decreased as WTR application rate increased. Tissue P levels were higher in the incorporated treatments than the surface applied treatments. However, tissue levels did not fall below the bahiagrass limiting value (0.15%). Based on this study, the use of WTRs is reco mmended as an amendment to soils with low P retention capacities that have been heavily im pacted with P or will r eceive high amounts of P e.g., a dairy sprayfield. 11
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CHAPTER 1 INTRODUCTION Nutrient enrichment was the leading cause of water degradation in the 1990s, polluting approximately 45% of waters in the United St ates (USEPA, 2000). N itrogen (N) and phosphorus (P) are the main nutrients of con cern; while they are naturally pr esent in the environment, they are also added as fertilizers in agriculture a nd home lawns and can enter aquatic systems through runoff or leaching. Eutrophication occurs when increased levels of nutrients in aquatic systems cause an influx of aquatic organisms and ulti mately a decline in dissolved oxygen (Brady and Weil, 2002). Under some eutrophic conditions, fishery, re creation and industrial water use is put under restrictions and surface waters may no longer be used for drinking water (Sharpley et al., 2001). Although N is necessary for the algae that cause the damages associated with eutrophication, environmental concerns have been focused on P since it has been shown as the limiting nutrient that most often causes accelerated impairment in aquatic systems (Sharpley et al., 1994). Florida has a large number of important wate r resources which contribute to many facets of life: groundwater supplies 90% of the drinking water in the state, sport fishing and bird watching bring millions annually to the economy and aesthetic beauty can be appreciated by all. In Floridas unique ecosystem, P from urban and agricultural activities has impacted many aquatic systems. The transfer of P downstream to other systems has created far reaching impacts (Reddy et al., 1999). For example, drainage wa ter enriched with P from the Everglades Agricultural Area drains into the Water Conser vation Areas and has cause d eutrophic conditions. The movement of P into freshwater bodies may l ead not only to eutrophication but also generally unhealthy biological conditions. 12
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The main sources of P in Florida are fertil izers, animal manures, municipal biosolids and effluents and composts. Applicat ion of manures and biosolids of ten occur at N agronomic rates resulting in P levels which are higher than ne cessary (McCoy et al., 1986). Long histories of high P applications have caused an accumulati on of P to the point where many crops do not benefit from added P. Although there is often little or no crop response from P fertilizers, many farmers in Florida still apply hi gher amounts than necessary. In 1996, over 50% of P fertilizers purchased in Florida were used in the South Florida Water Management District which is especially susceptible to P loading in aquatic systems (Re ddy et al., 1999). Florida has more than 14 million hectares of acidic soils most of which are sandy (He et al., 2005). These sandy soils are less capable of retaining P because unlike finer textured soils they do not contain large quanti ties of mineral components such as metal oxides and secondary phyllosilicates which aid in P retention (Sampl e et al., 1980). A study conducted on 96 Florida sandy soils showed more than 10% of the soil P was water so luble suggesting high leaching potential (Zhang et al., 2002). Harris et al. (1996) contend the ma in reasons for low P retention in Alaquods are (i) very low amounts of aluminosilicate and metaloxide clays in the E horizon and (ii) a seasonal shallow water table that results in the transport of P laterally into connected drainage ways. In 2007 Florida had 18,900 cattle farms and 440 dairy farms (USDA, 2008). Livestock operations across the nation are intensifying their practices and creating Concentrated Animal Feeding Operations (CAFOs). With this intens ification comes more ma nure production per acre of land. Before USEPA regulations, CAFOs appl ied manure at N agronomic rates resulting in excessive P levels and possible P loss scenarios (USDA-USEPA, 1999). The risk of P loss can be exacerbated with the poor sorb ing soils found in Florida. 13
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A number of best management practices (B MPs) have been designed to help control P loss from agricultural soils. Fertilizer applica tion rates, animal density, irrigation management, runoff and wetland retention systems are being mana ged to help reduce eutr ophication of aquatic systems (Bottcher and Rhue, 1984). While these m easures are helping to reduce P contamination in Floridas aquatic systems, increasing populations and inte nsively managed agricultural operations will place pressure to continually reduce the amount of P entering aquatic systems. Controlling erosion, lowering levels of fe rtilizers in agricultural production and implementing BMPs are approaches used to he lp prevent eutrophication (Troeh et al., 2004). Several physical means of reducing surface runoff to minimize P losses are currently in practice: conservation tillage, buffer strips, runoff wate r impoundments and riparian zones (Novak and Watts, 2004). Recently, a variety of chemical soil amendments have been suggested as means of increasing P retention by the soil and thereby reducing P losses. Aluminum (Al) and ir on (Fe) salts (Al2(SO4)3, FeCl3) have been shown to lower the amounts of labile P in the soil but often have high costs whic h detract from their potential widespread use (Moore and Miller 1994). Water treatment residua ls (WTRs) are by-products of the drinking water purification pr ocess that contain amorphous Fe or Al oxides or calcium carbonate as well as sediment and humic substances from the treated water (Makris et al., 2004). The Fe and Al compounds in WTRs contain r eactive hydrous oxides with substantial P-fixing capabilities (Elliott et al., 2002). The current methods of WTR disposal are expensive for water treatment municipalities and include storage in landfills and onsite lagoons and discharge into sanitary sewer systems (Dayton and Basta, 2001). Thus, other methods of disposal and utilization such as land applica tion are being evaluated. Ippolito et al. (2003) list some of the possible benefits of WTR land application: in creased N and organic carbon availability for 14
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plants, improved aggregate stability and water retention, better aeration and drainage capacity and increased cation exchange capacity for coarse textured soils. Numerous studies have looked at the characteristics and capa bilities of WTRs as a means of controlling P loss from leaching. Most of thes e studies have taken pla ce in a laboratory or a greenhouse under controlled condit ions. These methods are a good way to make initial evaluations of the tested material. There is concern in the agricultural sector of using an amendment containing Al and the effects it may ha ve on crop growth and production. There is a strong need to determine the effects WTR will have on soil P movement, plant P uptake and possible Al toxicity problems under field conditio ns. This study will address these concerns under field conditions. The objectives of th is study are to: 1. Evaluate the effects of Al-WTR applica tion method and rate on P forms in the soil 2. Determine the effects of Al-WTR app lication on the soil microbial population 3. Examine P leaching through groundwater monitoring 4. Evaluate forage yield and nutritive valu e as affected by Al-WTR application method and rate 15
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CHAPTER TWO LITERATURE REVIEW Phosphorus Phosphorus (P) is a macronutrient required by plants to provide and produce energy and is the second most limiting nutrient. In most plants it comprises up to 0.2% of dry weight (Schachtman et al., 1998), while legumes can have higher concentrations in the range of 0.40.6% (Lush, 1934). It is esse ntial to many cell components such as nucleic acids (DNA and RNA), phospholipids and adenosine triphosphate (ATP) (Schachtman et al., 1998). ATP is required for most processes within the plant that need energy in cluding the uptake and transport of nutrients. Phosphorus improves various plant physiological aspects such as nitrogen (N) fixation, photosynthesis and root development. Phosphorus deficiency can be observed by stunted, thin-stemmed and spindly plants. Some plants develop a purple color while extremely deficient P levels may result in the yellowing of leaves. Deficiencies also cause delayed maturity, sparse flowering and poor seed quality (Brady and Weil, 2002). The movement of P in the environment is strongly affected by the form of P. Inorganic P is normally found bound with aluminum (Al), iron (Fe), calcium (Ca) and magnesium (Mg) (Graetz and Nair, 1999). Inorganic P is the most common form taken up by plants and is rarely higher than 10 M in soil soluti on. The form of inorganic P ch anges according to pH. The dissociation pKs of H3PO4 are 2.1 and 7.2 for H2PO4 and HPO4 2-, respectively. Most P uptake occurs in a pH range of 5.0-6.0 making H2PO4 the most readily available form of inorganic P to plants (Schachtman et al., 1998). The Langmuir Equation is a mathematical model used to predict anion adsorption onto reactive surfaces. This equation shows that the av ailability of phosphate is often controlled by the amount of saturation at the sorption sites. As the sorption sites become saturated with anions 16
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the concentration of the anion in solution increases. Desorpti on of phosphate creates a condition of hysteresis in which desorption is much slower than adsorption to such an extent that it does not affect soil processes. Hysteresis has importa nt environmental implications because it allows the soil to hold onto phosphate it has sorbed even once its concentration decreases. This ultimately results in less leaching; however, it is onl y effective when soils have the ability to sorb phosphate or when the soil has not been oversaturated (McB ride, 1994). Soils can become loaded with high concentrations of P through agricultural management practices. Manure from local live stock productions is often applied as a fertilizer based on plant N requirements which results in P application ra tes that exceed plant needs (Sharpley et al., 1994). Excessive fertilizer usage and biosolids application can also load soils with excessive P (OConnor et al., 2002). Graetz and Nair (1999) reported that up to 80% of total P has the potential to be transported in heavily manure-impacted upland so ils. Repeated additions of P over time reduce the soils capacity to retain P (Reddy et al., 1999). Soils in the eastern Coastal Plain are characteristically highly weathered and coarse textured soils which have a lowered affinity to sorb P. When these soils with low P retention capacity are coupled with high loads of P, the ab ility to bind P is signi ficantly lowered (Novak and Watts, 2004). Florida has many low P sorbing soils and hydraulic conditions that allow for P to easily leach into bodies of water causing environmental problems (OConnor et al., 2002). Soil test P (STP) and degree of P saturation (DPS) are indicators often used to assess environmental risks associated with P applica tion. Neither indicator however accounts for the soils capacity to retain P. Although STP and D PS values may be low, applying high amounts of additional P could result in excess P not bound by the soil and induce risks to local water systems. Nair and Harris (2004) proposed usi ng a calculation to determine the soil P storage 17
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capacity (SPSC) which would account for both prev ious P loadings onto a soil and the capacity of the soil to retain a dditional P. They found that some Spodosols in Florida had low STP and DPS values but also low sorption capacities so loading these soils with P based on these two factors could pose environmental risks. Th e SPSC value would show potential risk of P application for soils with low sorption capacities. Phosphorus Retention by WTR The ability of WTRs to sorb P has been tested to determine if it can be used as an agent to prevent P runoff. Gallimore et al. (1999) applie d WTR to plots fertilized with two poultry litters at a rate of 6.72 Mg P ha-1. The WTR was applied at 44.8 and 11.2 Mg ha-1 on broadcast plots and 44.8 Mg ha-1 on a buffer strip. After simulated rain events, surface runoff was collected, analyzed and compared to control plots. Disso lved P was reduced 42.7% in the broadcast plots at the 44.8 Mg ha-1 WTR rate and 45.9% in the buffer strips when compared to the control. Land application of WTR did not in crease soluble Al or sediment in the surface runoff. Novak and Watts (2004) evaluated the use of WTRs for P retention on sandy coastal plains that have a high susceptibility to P leaching. Soils obtained from North and South Carolina were combined with several WTRs in blends of 0, 2.5, 5.0, 7.5 and 10.0% dry weight by mass. A calcium chloride solution with P concentrations ranging from 5,000 to 30,000 mg P L-1 was added to the soil and WTR mixtures. The WTRs proved to sorb 100% of P when the solution contained between 5,000 and 10,000 mg P L-1. The Pmax value determined for the soils alone was less than 1 mg P g-1 while the Pmax when WTR was incorporated with the soil ranged from 1.7 to 8.5 mg P g-1 depending on the composition of the WTR used. From this study it was concluded that WTR can increase a soils P so rption values and prospectively help P from moving to bodies of water. 18
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Alum (Al2(SO4)3) has been used to reduce P in a vari ety of environmenta l conditions. It has been applied in wetlands when sediments have been known to release P (Ann et al., 1999) and also to soils amended with poultry litter high in P (Shreve et al., 1995). The P retaining capacities of alum were compared to an Al-WTR because of the high cost of alum. The Al-WTR was nearly as effective as alum at reducing th e amount of P released when combined with a poultry litter. The advantages of the WTR incl ude lower costs and less dissolved Al released than alum (Makris et al., 2005). Other amendments have also been studied for their ability to reduce P movement. Haustein et al (2000) compared the efficiency of a WTR to a byproduct of the commercial alum production process, HiClay Alumina (HCA). An Al-WTR and HCA were a pplied at rates of 0, 2.2, 9.0, and 18 Mg ha-1 to plots with high to excessive so il test P levels (150 to 300 mg P kg-1 soil). Rainfall was simulated to produce runo ff one day, one month, and four months after application. The WTR was able to sorb P at a rate 20 times higher th an HCA most likely because WTR contained three times as much Al a nd more clay particles. This study also looked at Al concentrations in the runo ff. The rain event after one day showed greater amounts of total Al for the two highest WTR appli cation rates than the control sinc e the residue contained 47 g Al kg-1. Runoff Al concentrations after one and four months did not differ from the controls. Thus, controlling when WTR is applied may preven t greater levels of Al in runoff. Elliott et al. (2002) examined the ability of Al-WTR, Fe-WTR and Ca-WTR to prevent P leaching in a sandy soil amended with biosolids a nd triple superphosphate (TSP). Soil columns were set up with bahiagrass grown on an A hor izon of Immokalee sand and an E horizon of a Myakka series. WTR was applied at 56 Mg ha-1. The controls had 21% of total P leach from TSP and 11% of total P leach from the Largo ca ke biosolid over four months. When WTR was 19
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applied, the amount of leached P reduced to 3.5%, 2.5% and less than 1% of total applied P for Fe-WTR, Ca-WTR and Al-WTR respectively, ma king the Al-WTR the most effective in decreasing soluble P of the materials tested. Miyittah-Kporgbe (2004) investigated the eff ects of application rate and the level of mixing an Al-WTR with the soil on P leaching. Leaching rates determined through a column study showed a reduction by 87 to 99.7% when Al-W TR was mixed completely with 15 cm of soil; partial mixing with 7.5 cm of soil only resulted in 40 to 58% less leaching than control columns with no applied WTR. More P reten tion was accredited to more contact with the amendment. Runoff from rainfall simulations sh owed that when Al-WTR was surface applied, soluble P concentrations were less than the critic al threshold of 0.03 mg L-1. The leachate concentrations when WTR was surface applied we re above the critical threshold because of limited contact with the amendment. These studie s showed that higher surface application rates can be as effective as using lower quantities of Al-WTR mi xed throughout the soil profile. Therefore, Al-WTR should be appl ied and distributed according to anticipated P losses. Surface applications will help prevent runoff losses and mixing the amendment will help prevent leaching. In a study by Silveira et al. (2006) the efficiency of WTR mi xed within the entire profile was evaluated by observing soluble reactive P (S RP) concentrations in leachate. WTR was added to a manure-impacted Immokalee soil at rates of 0, 25, 50 and 100 g kg-1. The WTR in the soil columns was either left unincorporated or mixed within the top 7.5 or 15 cm. Water extractions through the columns were analyzed for SRP. Control samples had an average SRP concentration of 38 mg L-1. In columns with WTR in the top 7.5 cm, SRP decreased to 19 mg L-1 and when WTR was in the entire 15 cm SRP further decl ined to 2 mg L-1. Thus, results 20
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showed deeper incorporation of WTR increased P immobilization. Rew (2006) showed similar results in a rainfall simulation involving the e ffects of an Al-WTR on manure impacted soils with a fluctuating high water table. Boxes were filled with manure-impacted soils high in P and either mixed with WTR or had WTR applied at th e surface at a rate of 2.5% dry weight by mass. Soluble P in runoff was more effectively re duced on the surface applied treatments while incorporating the WTR was better at reducing soluble P in leachate and subsurface flow. Both of these studies imply that incorporating the WTR allows for more contact with the impacted soil and so better reduces downward movement of P than surface application. It was suggested that to reduce P in runoff and leaching, WTR would need to be surface applied after being incorporated (Rew, 2006). Ippolito et al. (2003) reviewed the relati onship between time and P desorption from a WTR. Water treatment residual samples with a given load of P were shaken in a calcium chloride solution for various lengths of time and analyzed to observe how much P was desorbed from the amendment. After a linear decrease in the amount of P sorbed (approximately 28 days), the concentrations of P being desorbed quickly leveled off. The quick decline in P desorption may represent P that was adsorbed to variable ch arged surfaces and the leveling off of desorption may be a result of more strongly precipitated calcium phosphates. The long term effects of P bound by WTR have not been extensively studied. In one study by Agyin-Birikorang et al. (2 007) the P bound by an Al-WTR was found to be stable after 7.5 years. A rainfall simulation demonstrated that WTR reduced dissolved and bio-available P by more than 50% compared to controls. Ther efore, it was shown that after 7.5 years the P associated with WTR should not contaminat e local waterways by runoff or leaching. 21
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WTR-Induced P Deficiency in Plants Phosphorus deficiency in plants is a concer n when applying an Al based WTR. Because of its chemical makeup and high concentration of Al, WTRs may sorb the P available to and needed for plants. Codling et al. (2002) applie d WTR to three different soils at rates of 0, 10, 25 and 50 g kg-1. After seven weeks, three cycles of wheat ( Triticum aestivum L.) were grown. At the 10 g kg-1 WTR application rate, biomass yield was not influenced for all the soils. Two of the three soils showed lower biom ass yields at the 25 and 50 g kg-1 WTR application rates. These two soils had a greater sand content than the unaffected soil. Tissue P concentration fell to deficient levels (<1.5 g kg -1) at the 25 and 50 g kg-1 application rate in the second and third cropping cycles although no deficiency symptoms were visual. Re sults from the study suggest WTRs can be applied at rates up to 10 g kg-1 without reducing yield production. Heil and Barbarick (1989) reported in creased sorghum-sudangrass ( Sorghum bicolor ) growth above the control when WTR was applied at 5 and 10 g kg-1. When application rates increased to 15 and 25 g kg-1, there was a 1.4 to 4.3 fold decline in plant yields. Ippolito et al. (2002) studied the effects of combining WTR with biosolids on two range grasses. Before testing it was assumed P availa bility would be reduced because of the chemical composition of WTR and thus P was supplemente d through the application of biosolids. However, even when the two amendments were co-applied, P availability was reduced for blue grama (Bouteloua gracilis ) and western wheatgrass ( Agropyron smithii ) observed by the lowering of tissue P. When WTR was applied al one, results showed increased Al uptake but no effects on dry matter yield or P uptake. Cox et al. (1997) performed a greenhouse study to determine the effects of alum sludge application on P availability to wheat ( Triticum aestivum L .) in a fine-loamy, Ultisol. Alum sludge is a byproduct of the drinking water purification process using Al2(SO4)3 14 H2O and 22
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which has low solids content (1-2%). Surface applied alum sludge had little effect on P availability while incorporating the sludge into the entire soil volume did show a decrease in P availability in the significantly decreased dry matter yields and plant P uptake. When the sludge was incorporated, Al compounds retained soil P that was otherwise loosely bound soil P and available to the wheat. It was suggested that higher P fertilizer requirements will be needed on soils amended with alum sludge. An Fe-WTR was amended with P to determin e if adding P could prevent P deficiencies in plants that may be caused by the WTR The Fe-WTR was also amended with a cationic polymer, Magnafloc 572C, before being applie d to sorghum-sudangrass. The amount of P needed to make the Fe-WTR a supplier of P was only 14.6 to 19.4 g P kg-1. This low P rate was partially a result of P release from the cationic pol ymer. The cost of adding P to WTRs to make them a supplier of P will vary depending on the am ount of Fe or Al, but in this study it was more economical than placing the WTR in a landfill (Hyde and Morris, 2004). In a rainfall simulation, the effects of an Al-WTR at a 2.5% dry weight by mass application rate were observed for stargrass ( Cynodon spp). Shoot growth, root biomass, and forage P, N and Al concentrations were not affected by the WTR (Rew, 2006). An Al-WTR was applied in combination with poul try litter manure under field conditions to determine if the WTR amendment would affect the yield an d nutrient concentr ation of corn ( Zea mays L.). The poultry litter was amended with WTR at rates of 0, 5.6 and 11.2 Mg ha-1 before being applied to the soil. The two sites used for the study were classified as an Ultisol and an Entisol. Corn grain yield and mineral nutrients (Zn, Mn, Fe, Ca, Mg, and K) in the leaves and grain were not affected by the addition of WTR. Tissue P levels remained above adequate growth levels for corn (2.0 g kg-1) and were similar to control plot leaf P concentrations, in dicating adequate levels of 23
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available P. Leaf Al concentrations were not affected by WTR and did not reach toxic levels (Codling et al., 2007). A simila r study was conducted by Codling et al. (2002) on wheat grown in a greenhouse where P deficiencies were obser ved when the WTR application rate was 25 and 50 g kg-1. The differences in P uptake between gree nhouse and field conditions were attributed to the confining volume of growing pots. U nder greenhouse potting conditions, the volume of soil to supply P is limited (Codling et al., 2007). A study by Oladeji et al. (2006) evaluated the effects of an Al-WTR on bahiagrass ( Paspalum notatum ) in a glasshouse study. The WTR was a pplied at three rates (0, 10, and 25 g kg-1) on P deficient soils in combination with va rious P sources applied at N and P agronomic rates. Bahiagrass tissue P concentrations fell between 1.5 to 6.0 g kg-1, right around the critical P value of approximately 2.0 g kg-1 used in this study. Dry matte r yields were not affected by WTR application. From this study it was conc luded that in order to avoid agronomic deficiencies, WTR application rates should be based upon the Al Fe and P composition of the soil, WTR, and P-source. Applying WTRs so so il P storage capacity is at zero allows for P uptake in plants to be minimally impacted and increased environmental benefits such as less P leaching. Aluminum Aluminum makes up 7% of the earths crust by mass and is the most abundant element in the crust (Delhaize and Ryan, 1995). It is found in all plants and some more tolerant species may contain more than 0.1% of Al by dry weight. Tissue samples from grass tops contained 50-3410 g Al g-1 by dry weight (Kabata-Pendias and Pe ndias, 2001). Although the physiological function of Al in plants is not clearly known it may be beneficial to some plants in small quantities in its ability to activate some enzyme s and control plasma and membrane permeability. 24
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Aluminum toxicity is one of the major probl ems and growth limiting agents in acidic soils. Toxicity occurs when the pH falls be low 5.5 and becomes increasingly damaging below 5.0 (Foy et al., 1978). The trivalent form of Al (Al+3) dominates solutions when the pH is below 5.0; Al(OH)+2 and Al(OH)2 + become more prevalent as pH increases. The trivalent form is assumed to be the phytotoxic form of Al because ot her trivalent cations are also toxic to plants and because toxicity is associated with acidic soil conditions (pH less than 5.0) (Delhaize and Ryan, 1995). Taking into consideration that WTRs are often buffered at 7.0 and agricultural fields are often maintained above pH 5.5 for opt imal growing conditions, Codling et al. (2007) contend Al toxicity should not issue problems since Al+3 solubility decreases as pH approaches 5.2. Soils high in organic carbon content may form Al complexations further reducing toxicity possibilities (Hsu, 1976). Acidification increases Al and manganese so lubility, thereby decr easing root growth (Jarvis, 1987). The process of soil acidification is mainly ca used by heavy rains which strip major cations from the soil. It can also result from using acidifying fertilizers (Mora et al., 1999). Aluminum toxicity causes less P uptake into the plant and also disables its movement within the roots (Edwards, 1991). Damage due to Al toxicity arises fr om decreased nutrient uptake and transport of other vita l nutrients, especially Ca, potassium and Mg, as well as from imbalanced cation to anion ratios (Kabata-Pe ndias, 2001). Nichol et al. (1993) observed the effects on cation uptake at Al levels of 100M. Calcium uptake was inhibited by 69%, ammonium by 40% and potassium by 13%. Possi ble mechanisms of reducing cation uptake include the Al+3 ions blocking or binding to negative charges in proteins of the plasma membrane. Even in micromolar concentrations, Al can cause stress in plants observable by the 25
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restriction of root growth which later inhib its plant growth (Delhaize and Ryan, 1995). The obstruction of root growth can cau se the plant to experience less water and nutrient uptake since roots do not penetrate the soil as extensively (Strid, 1996). Aluminum toxicity can be minimized by addi ng various solutes to the solution. Hydroxyl ions or alkaline salts reduce Al activity instead of Al concentr ations but prove to be helpful nonetheless. Raising the solution pH also decreases Al toxicity (Parker et al., 1989). While Al toxicity is strongly affected by pH, the amount of extractable Al in so il and soil solution is partially determined by mineralogy and the am ount of weathering (Fox et al., 1991). For instance, solution Al in an Ultisol had toxic values around 0.62 mg L-1 while Al concentrations of an Oxisol were around 0.05 mg L-1 under the same growing conditions. Mineralogical structure helps to explain these differences in Al solubility. More highly weathered soils normally have lower Al solubility. Edwards (1991) studied interactions betw een soil acidity, liming and P additions to spring barley, winter wheat, woilseed rape and grass common to Scotland. Yield reductions were witnessed with increases in acidity. By ma intaining P soil levels adequate for crop growth, the effects of Al toxicity were noticeable at pH values below the optimal growing conditions of 5.5. In a study conducted by Dayton and Basta (2001 ), WTR was considered as a substitute for the top layer of soil. Many properties were analyzed including cation exchange capacity, nutrient levels, plant available water and potential toxicities. The only possible toxicity problems pertained to levels of nitr ite which if greater than 10 mg kg-1 are considered toxic to plants. Water soluble Al levels fell below the regulatory value of 1.0 mg L-1 in all of the 14 WTRs tested. 26
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Phosphorus and Water Movement in Spodosols There are approximately 4.5 million acres of Spodosols in Florida (Li et al., 1997). Spodosols are characteristically nearly level, poorly drained, sandy soils with high seasonal water tables and an organic Bh horizon. Naturally these soils are very infe rtile but can be highly improved for crops or pasture if managed correctly (Soil Survey Staff, 1996). Spodosols are formed from intensive acid leaching in moist areas. Acid organic compounds bind Al or Fe and carry them down the soil profile by percolating waters. A subsurface spodic horizon (Bh) forms when Al a nd Fe precipitate beneath an albic horizon. The organic compounds make this horizon black in color (Brady and Weil, 2002). Spodosols can cause a perched water table to exist. This can result in surface and subsurface runoff losses because P does not come into contact with the Bh horizon where the Al and Fe have the capability of forming precipitates and preventing P loss (Burgora, 1989). Spodosols found in the Myakka series in Florid a have an average water table depth of 76 cm (Allen, 1987). Nair et al. (1999) looked at how P retention was affected by the changing aerobic and anaerobic conditions that occur with a fluctuating wate r table in Myakka soils. The sandy upper A and E horizons allow P to move in to the spodic horizon where it can become trapped due to higher contents of Al and Fe. Anaerobic co nditions led to equilibrium P concentrations which were 30% greater than aer obic conditions and a P sorption maximum that was less than 25% of aerobic conditions. These statements suggest P retention decreases when the water table conditions are hi gh (above the spodic horizon). Villapando and Graetz (2001) similarly found that even though most of the P content of the Bh horizon was associated with Al and Fe, a significant percentage was still in a hi ghly mobile form which could be transported to nearby waterways with lateral movement above th e spodic. Conditions in which P could move laterally above the Bh horizon depend on the P lo ading of the spodic: non-manure impacted soils 27
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such as pasture soils have less P and so the possib ility of P moving out of the system is less than a highly impacted soil (N air et al., 1999). Soil Microorganisms Microorganisms encompass only 1-3% of soil organic matter (Jenkinson, 1988) yet there can be as many as 109 bacterial cells per gram of soil (T orsvik et al., 1990). Microorganisms have many vital roles in the soil environment. They decompose organic matter thereby releasing substantial amounts of nutrients. They are majo r constituents in biogeochemical cycles (carbon, nitrogen, sulphur) and also help in environmental processes such as the degradation of pollutants (Curtis and Sloan, 2005). Soil microorganisms are considered an excellent biological soil qual ity indicator because they respond quickly to environmental changes (Tur co et al., 1994). They also play a vital role in the cycling of N and C (Nanni pieri et al., 1990). Specificall y, nitrification is a functional parameter in assessing soil quality because it is ca rried out by specialized bacteria which are very sensitive to environmental changes, stress and toxic substances (Bollmann, 2006). Nitrification, the reduction of N to nitrate (NO3 -), is normally a twostage process with ammonia being oxidized to nitrite, and th en nitrite quickly being oxidized to NO3 (Prosser, 2007). Nitrification is affected by a number of factors which can interact to affect the overall process. Nitrifiers must be present, and because they are aerobic organisms oxygen must also be available. Nitrification is optimal when soils ar e near field capacity or when 60% of pore spaces are filled with water. The most important regu lating factor is substrat e availability, namely ammonium (Myrold, 1999). Soil pH is another infl uence on nitrification. Most soils below pH 4.5 have negligible nitrification ra tes because nitrifiers generally wo rk best at neutral pH. It has been shown however that nitrification can occur at pH below 4.0 in forest soils. One possible explanation for nitrification under these low pH conditions is that N rich material in microsites 28
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release ammonium, increasing the pH within the microsite. Another is the presence of more acid tolerant nitrifiers. The best pos sible explanation is that the lack of Al toxicity in organic and some forest soils allows nitrif ication at even lower pHs (Paul and Clark, 1996). Temperature, water potential, salinity and nutrient availability also affect nitr ification rates (Myrold, 1999). Aluminum negatively affects soil bacterial growth and overall numbers of rhizobium (Glenn and Dilworth, 1991). The mechanisms which specifically harm the bacteria are unknown. Soybeans and cowpeas have shown decreas ed levels of N fixing capabilities when grown in combination of low pH and toxic levels of Al (Mookherji and Floyd, 1991). A study on forest soils reported decrea sed nitrification rate s as potassium chloride extractable Al increased (Gilliam et al., 2005). Alexander (1980) proposed that acid precipitation aff ects nitrification more than other N cycle phases. The effects of nitrification as impacted by acid preci pitation were studied by Bitton et al. (1985). Acid rain of pH 3.7 and 3.0 along with a control of 4.6 was applied to 75 m2 plots on a typical Florida sandy soil. Nitrification rates proved to be the most sensitive to the acid rain and were reduced from 0.16 mg NO-3-N kg-1 d-1 on control plots to 0.08 and 0.06 mg NO-3-N kg-1 d-1 for acid rain with a pH of 3.7 and 3.0, respectively. There is little information on the imp act that WTR application has on soil microorganisms. In one study, six WTRs from S outh Africa were added to two soils at various rates and soil respiration was monitored. In general, the soils amended with WTR showed significant increases in soil respiration. The e ffects were less visible after time, indicating increased microbial activity may not be a long-te rm result. Based on so il microbial indicators alone, WTR could be applied at a 300 Mg ha-1 rate without negatively impacting soil quality (Pecku et al., 2006). 29
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Sikora (2004) investigated th e effects of Al-WTR on plan t residue decomposition. The WTR was applied to two Ultisol s at rates of 0.5, 2.5 and 10 g kg-1. Carbon labeled soybean leaves and stems were added. Lime (CaO) was also added to evaluate the effects of raising the pH. There was no effect on soybean residue decomposition by WTR or CaO addition. Salinity increases were negligible and did not affect the other soil characteristics studied. It was recommended that the WTR application rate shoul d have the same metal molar concentration as soil P molar concentration. Bahiagrass Bahiagrass is a warm season perennial common thr oughout Florida. It can sustain high temperatures and low periods of rain which often limit the growth of other grasses. In Florida, bahiagrass covers approximately 2.5 million acres making it the highest single used grass pasture species (Chambliss and Adjei, 2006). It is e used extensively in Fl orida because of its ability to tolerate a wide range of soil conditions, produce mode rate yields even when the soil has low fertility, establish easily from seed, withstand close grazing and is not regularly affected by insects and disease. Mylavarapu et al. (2007) determined the limiting value of bahiagrass to be 1.5 g kg-1. The limiting value is defined as the ti ssue concentration above which added P will have no effect on plant yield. Aluminum has no known biological value to animal nutrition, thus it is almost never added to the diet. It can be inge sted through soil or grass. In some animals, Al at certain levels can cause P deficiencies. Toxicity is altered by the solubility of Al and quant ity of P in the diet. Thompson et al. (1959) proposed ruminant P leve ls were not as negatively impacted by the consumption of excess Al. Based on results fr om numerous studies, th e Board on Agriculture (1980) set the maximum tolerable level for Al for sheep and cows at 1,000 ppm. 30
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Since bahiagrass is such an important and wi dely used forage in Florida cattle production, it is vital to consider the impact WTRs may have on forage yield and tissue nutrient concentrations, namely Al. Phosphorus leve ls should not drop below levels which would decrease yields. Aluminum can affect animal dietary needs and should also be monitored, not only in the amount consumed in the forage but also in the soil. 31
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CHAPTER THREE MATERIALS AND METHODS Site Description and Experimental Design The study was performed on an established bahiagrass ( Paspalum notatum ) pasture at the Range Cattle Research and Education Center (RCRE C) in Ona, FL (27' N, 81' W). Main plots (15.24 X 6.10 m) were divided into s ubplots (3.05 X 6.10 m) a nd separated by 3.05 m buffer strips (Fig. 3-1). A ra ndomized block, split-plot design used application method (soil incorporation and surface appl ied) as the main plot and WTR rate (0, 35 and 70 Mg ha-1) as the subplot. Each treatment was replicated four tim es. Aluminum WTR was incorporated in the top 10-15 cm of soil in the designated plots by disking. The bahiagrass was allowed to grow in from the existing tilled-in material. Elliott et al (2002) determined pract ical and effective WTR application rates to be between 20 and 100 Mg ha-1 through column leaching studies and thus rates for this study were chos en within this range. N N Figure 3-1. Plot design and layout The shaded regions indicate surface applied treatments and non-shaded regions indicate incorporated tr eatments. White boxes are the controls, light gray boxes are the 35 Mg ha-1 WTR application rate and the darker gray boxes are the 70 Mg ha-1 WTR application rate. 32
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The soils on the site are from the Myakka soil series (sandy, siliceous, hyperthermic aeric alaquod). This soil series is found extensiv ely throughout Florida, covering more than 1.5 million acres. In Hardee County, it makes up more than 55,000 acres which is more than 13% of the countys soils (Soil Survey Staff, 1996). The study site had very low initial P concentrations (Mehlich-1 P = 2.1 mg kg-1 for the A horizon). Additional P was surface applied in the form of triple superphosphate (TSP) with a hand-held fertilizer spreader to all plots at a rate of 224 kg P ha-1. Applying TSP at high levels allowed for the movement of P throughout the soil pr ofile to be evaluated. It also simulated circumstances when P would be applied at high levels to the soil surface, such as in the application of manures or biosol ids at N agronomic rates. A tim eline of the various activities on the plots is shown in Table 3-1. 33
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Table 3-1. Dates and descripti ons of field activities. Aug. 15-17, 2006 Laid plot perimeters and collected soil samples. Sept. 9, 2006 WTR delivered from Manatee County Water Treatment Plant. WTR sieved to 0.64 cm. Jan. 4, 2007 Initial forage samples taken. Moisture content of sieved WTR determined. Jan. 5, 2007 Plots mowed and forage removed. WTR weighed into bags for application. Jan. 11, 2007 Al-WTR applied to plots w ith a drop fertilizer spreader Jan. 12, 2007 WTR incorporated into necessary plots. Fertilizer combination spread (34-0-60). March 26, 2007 Wells placed in the middle of plots 60 cm deep. April 23, 2007 Nitrification soil samples collected and then TSP applied at 224 kg ha-1 per plot. May 24, 2007 Forage harvested. Fertilizer combination spread (34-0-60). June 20, 2007 Forage harvested. Fertilizer combination spread (34-0-60). July 20, 2007 Forage harvested. Fertili zer combination spread (34-0-60). Aug. 20-21, 2007 Second soil samples collected. Forage harvested. Fertilizer combination spread (34-0-60). Sept. 20, 2007 Nitrification soil samples and groundwater samples collected. Forage harvested. Oct. 2, 2007 Groundwater samples collected. Water Treatment Residual An Al-WTR was selected for field testing be cause of its effectiveness in controlling P leaching when soluble P was in contact with th e WTR. Aluminum WTR was acquired from the Manatee County Water Treatment Plant in Braden ton, FL. Previous studies compared an AlWTR from this plant to an Al-WTR from Okeechobee, FL, and to other various soil amendments. The ability of each amendment to sorb P and prevent leaching and runoff was evaluated. The other amendments were not recommended for an a ssortment of reasons including ineffective P sorbing capacities, high concentrations of potentially detrimental nutrients, threats of soil salinity and high cost. The Manat ee WTR was recommended over the Okeechobee WTR for field evaluation because of its drier condition, ease of handling a nd the large quantities 34
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produced yearly (about 4,000 Mg yr-1). The recommended rate of application was 2.5% dry weight by mass (OConnor, 2005); th e rates of application for this study were 1.5 and 3.0% dry weight by mass. The initial size of the WTR particles varied largely from clay size upwards to 12 cm so they were passed through a 0.64 cm mesh to obtain better uniformity. The moisture content of the WTR was not high enough to cause a problem wh en sieving and so it was not previously air dried. Sieved WTR was stored at the study site under visqueen tarps until application. Five random samples were taken the day prior to WTR application to determine the average moisture content of the Al-WTR. Given the 30.3% moisture content, th e amount of WTR needed for the 35 and 70 Mg ha-1 application rates was 93 and 186 kg per plot, respectively. The WTR was applied with a drop fertilizer spreader to assure an even distribution. Soil Sampling Two soil samplings were performed during the course of the study. An initial sampling before any treatment was applied was taken in August 2006 and the second sampling was taken in August 2007. Two holes were augured to a depth of one meter for each plot. The profile was carefully laid out in rain gutte rs and depths noted at each cha nge in horizon (Table 3-2). One composite sample from each horizon was then taken from both profiles. The A horizon was split into two depth increments, one from the top 8 cm and one from below 8 cm. This technique was followed to better quantify differences due to the incorporation process. All soil samples were placed in plastic bags and kept on ice until th ey were transported to Gainesville, FL, for laboratory analysis. All samples we re air-dried before analysis. 35
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Table 3-2. Depths to the bottom of the soil horizons sampled. The A horizon was sampled from 0-8 cm and 8 cm to the bottom of the horizon. Soil Horizon Average Depth (cm) A 8 A 21 E 54 Bw 75 Bh 83 Bt 100 Soil samples were collected on April 23, 2007 and September 20, 2007 to determine nitrification potential. In these samplings, the top 5 cm of the A horizon were placed in sampling bags and stored in a cooler. A 1.3 cm diameter stainless steel probe was pre-marked to 5 cm and two samples from each quartile of the plot were collected. Forage Sampling An initial forage sampling was conducted in January 2007 before any treatments were applied. Two 51cm2 tracts were used to take forage samples from each plot. The grass was cut to approximately 3 cm above th e ground with hand shears. Plot s were harvested every 30 days from May to September, 2007, with a flail mowe r and a dump wagon attachment to collect and weigh harvested forage. Hand drawn samples at each harvest were dried at 105F, moisture content calculated and sieved to 2 mm for analysis. After each harvest, plots were fertilized with ammonium nitrate and potassium chloride at rates of 89.7 kg N ha-1 and 44.9 kg K2O ha-1, respectively. Groundwater Sampling A 60 cm PVC well was placed above the spodic horizon in the center of all plots. There were slits in the lower 10 cm to allow wate r to enter the well. The goal was to collect groundwater samples when a perched water tabl e existed to observe concentrations of P leaching. Due to an unusually dry early summer, samples were not able to be collected until late 36
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in August and September. The RCREC receive d 7.4 cm of rain in May through August of 2007, while the average amount of rain for the previous 4 years was 13.85 cm (FAWN). Water samples were collected using a battery operated vacu um pump. Prior to sampling, standing water in the wells was purged to assure that fresh water was being sampled. After approximately one hour, the wells were recharged and 200 mL samples were collected into Nalgene bottles. Samples were preserved with H2SO4 (1 drop per 20 mL sample). Samples were placed on ice before refrigera ted in the laboratory. The groundwater table depth was measured usin g a pressure transduc er to measure the potential of water at varying depths (modeled from the design of Schumann and MunozCarpena, 2002). The transducer was calibrated by using a bucket of water at known water levels and recording the voltage. A regression was then ma de from several values and an equation was produced to convert voltage read ings to water depth. A Hobo da talogger was set to record voltage readings in the fi eld every 15 minutes. Analytical Methods Soil All soil samples were air dried before analysis Water extractable P, Mehlich-1 P, Fe and Al, pH and the soil P storage capacity were determined for all soil samples. Water extractable P (WEP) was determined using a 1:10 soil to solution ratio (Kuo, 1996). Three grams of soil was weighed into 50 mL centrifuge tubes and 30mL of distilled deionized water (DDI) was added. The tubes were placed on a mechanical shaker for one hour and then passed through a 0.45 m filter with a vacuum. After extraction the samples were refrigerated. Analysis with a TechniconTM Autoanalyzer was performed within 48 hours. Mehlich-1 extractable P, Fe and Al were determined on all soil samples (Mehlich, 1953). Five grams of soil was weighed into a 50 mL ce ntrifuge tube and 20 mL of Mehlich-1 solution 37
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(0.05 N HCl and 0.025 N H2SO4) was added. The tubes were shaken for five minutes and immediately filtered through Whatman #41 filte r paper. Samples were analyzed on a TechniconTM Autoanalyzer. The pH was determined by weighing 5 gram s of soil and adding 10 mL DDI water giving a 1:2 soil to solution ratio. The solution was st irred gently and after 30 minutes a reading was taken with an Orion pH electrode. Nitrification Potential The nitrification potential of the soils was de termined using the shaken soil slurry method (Hart et al., 1996). The method was performed th e day after soil collecti on. Fifteen grams of field moist soil was added to a 250 mL Erlenmeyer flask. One hundred milliliters of a solution containing 1.5 mM NH4 + and 1.6 mM PO4 -3 was added to each flask. The original procedure called for 1.0 mM PO4 -3 but this was increased to account for the low soil acidity. The buffer solution was adjusted to pH 7.2 by adding NaOH dropw ise. Parafilm with a slit cut on top was placed securely over each flask to allow oxygen exchange but minimize evaporation. Samples were placed on a shaker at 1,000 rpm in an incuba tor at 25C. Approximately 10 mL of slurry was collected at 2, 4, 8, 20 and 24 hours and filtered through Whatman #40 filter paper. Samples were stored in scintillation bottles and aci dified with one drop of concentrated H2SO4. Analysis for nitrate-N was performed on a rapid flow analyzer using EPA Method 353.2. The rate of nitrate-N production (mg NO3 --N L-1 h-1) was calculated by linear regression of solution concentration versus time. The gravimetric soil water content was determined for the same soil samples (mass of water / mass of dry soil) and used to calculate the dry soil potential nitrification rate. 38
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Groundwater Groundwater samples were analyzed for sol uble reactive P (SRP) within 48 hours of collection. Twenty milliliters of the sample was filtered through a 0.45m filter before analysis. Samples were analyzed using a TechniconTM Autoanalyzer by EPA Method 365.1. Forage All forage samples were dried and then gr ound to 2 mm with a Wiley mill. Neutral detergent fiber content (NDF), N (crude protein), P, Al, Ca and Mg were determined for forage samples. Neutral detergent fiber estimates the plants ce ll wall contents and is the best indicator of how much forage an animal will potentially co nsume. To determine the NDF, 0.50 g of grass was weighed into a bag, the weight was recorded and the bag sealed (Golding et al., 1985). The sample was then boiled in a neutral deterg ent solution for one hour leaving the cell wall constituents. The bags were soaked in acetone and placed in an oven for 2-3 hours to complete the drying process. The dry bags were weighe d and the NDF was reported on a dry matter basis. Tissue nutrient content (N, P, Al, Fe, Mg and Ca) was determined using a modification of the total Kjeldahl method (Gallaher et al., 1975). Samples were weighed to 0.25 g and placed in 75 mL volumetric glass tubes. A catalyst (containing 9:1 K2SO4:CuSO4), 6 mL of concentrated H2SO4 and 2 mL of H2O2 were added and samples were digested at 375C for at least 4 hours. After samples cooled they were brought to volume with DDI water, mixed thoroughly, filtered and stored in 20 mL scintillation vials. Nitr ogen and P in the digestate was determined by semiautomated colorimetery using a TechniconTM Autoanalyzer by TKN Method 351.2. Crude protein was calculated by multiplying the amount of N in the sample by 6.25. Aluminum, Fe, Mg and Ca were determined by atomic adsorption spectrophotometry. 39
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Quality Assurance and Quality Control Quality assurance and quality control (QA/QC) were met with 10% repeats and blanks for each procedure. Quality control was determin ed by certified external standards and standard calibration curves. A standard deviation of 10% was required for all repeats and quality control checks. Statistical Analysis Statistical analysis was executed using SAS version 8.2 1999-2001 (SAS Institute Inc., Cary, North Carolina, USA). The Waller-Duncan procedure was used to compare the mean values for application method and applicati on rate. ANOVA was used to determine the individual variable responses (application method and rate) and interactions between the variables. A single degree of freedom was used to explore the general mean contrast between application rates. 40
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CHAPTER FOUR RESULTS AND DISCUSSION Soil Properties Initial Soil Characteristics The soil on the experimental site was classified as Myakka fine sand based on soil survey information (USDA-Soil Conservation Service, 1984). This was s ubstantiated during the sampling process. Twenty-two of the 24 plots had a spodic horizon within the one-meter depth sampled. Initial soil WEP and Mehlich-1 P average concentrations were relatively low and decreased with depth through the A and E horizons and then Mehlich-1 P slightly increased in the B horizons whereas WEP remained low (Table 4-1). An anomaly was found in the A horizon (0-8 cm) between WEP a nd Mehlich-1 P concentrations which were 9.0 and 2.1 mg kg-1, respectively. It is uncommon for WEP concentrations to be higher than Mehlich-1 P concentrations because extracting P with an acid solution should extract more P than the water extract. There is no logical explanation for this observation and it was not observed in posttreatment sampling. Mehlich-1 P concentrations in the B horizons were approximately 30-fold greater than in the A and E horizons. Mehl ich-1 P concentrations less than 10 mg kg-1 in the A horizon are considered very low for agronomic crops as well as bahiag rass and P fertilization would be recommended for this site (Kidder et al., 2002). These concentrations are slightly lower to those found in forage fields in South Florida (Graetz and Nair, 1995). 41
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Table 4-1. General characteristics in soil sample s taken prior to amendm ent application (August 2006) averaged for all plots. Soil Horizon Soil pH Water Extractable P Mehlich-1 P Mehlich-1 Al Mehlich-1 Fe Al:Fe Ratio ---------------------------------------mg kg-1---------------------------------------A (0-8 cm) 4.59 9.0 3.8 2.1 1.2 22 9.9 4.6 A (8-21 cm) 4.42 3.0 1.6 1.4 2.7 35 14.7 5.0 E 4.36 0.8 0.8 0.7 1.9 20 9.0 4.5 Bh 5.62 1.3 1.4 29.1 16.7 334 71.7 9.7 Bw 5.40 1.9 3.3 36.1 16.6 287 54.1 11.0 Bt 6.37 2.0 0.9 38.4 19.5 131 25.4 10.7 Mehlich-1 Al and Fe concentrations we re approximately 10 and 4 fold greater, respectively, in the B horizons than in the A a nd E horizons (Table 4-1). This accumulation of metals is a result of spodic horizon formation when metals and carbon are redistributed from one zone to another (Franzmeier et al., 1965). Th e Al:Fe ratios in the A-E and B horizons were approximately 5 and 11, respectively. It is typical for Fe concen trations to be lower than Al concentrations in poorly drained Spodosols in Fl orida (Sodek et al., 1990). These spodic Al and Fe values are similar to those found in a South Fl orida beef pasture by Nair and Graetz (2002). Aluminum Water Treatment Residual Characteristics The moisture content and pH of the Al-W TR were 30.3% and 6.8, respectively (Table 42). Mehlich-1 Al and Fe concen trations were 1,007 and 17 mg kg-1, respectively. A recent study using material from the Manatee County Water Tr eatment Plant found that nearly all the Al and Fe was oxalate extractable (OConnor, 2005). Ox alate extracts amorphous Al and Fe which are highly reactive to P (McKeague et al., 1971). Anot her study also using material from the same source at an earlier date f ound that approximately 80% of the Al was amorphous (MiyittahKporgbe, 2004) and the total Al concentration was 89.1 g kg-1 (OConnor et al., 2002). The WTR had a substantial total P content (1,300 mg kg-1). Although this will result in a large amount of P added to the soil, there is little chan ce of P release from the WTR since WEP 42
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concentrations were below the detection limit. Most of this P is bound by the extensive amount of Al. Desorption of P from an Al-WTR was reported to be less than 1% (Makris et al., 2004). Table 4-2. General characteristics of the Manatee County Al-WTR. Moisture Content pH* Mehlich-1 Al Mehlich-1 Fe Mehlich-1 P Total P** Water Extractable P -----------------------------------mg kg-1-----------------------------------30.3% 6.8 1007 17 2 1300 BDL*** Soil:Solution ratio of 1:2 ** Total P determined by ignition method (Anderson 1976) *** Detection limit was 0.02 mg L-1 Soil pH Soil pH eight months after WTR application was affected by application method for the A (0-8 cm) and A (8-21 cm) horizons and nearly a ffected by WTR application rate in the A (0-8 cm) horizon (Table 4-3). There were no effects of WTR application method or rate in the E and B horizons. In the A (0-8 cm) and A (8-21 cm) horizons differences resulted from application method in which the incorporated treatments had a higher pH than the surface applied treatments (Table 4-4). Table 4-3. The p-values for the pH data using ANOVA and general mean contrast (single degree of freedom). Soil Horizon Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 A (0-8 cm) 0.009 0.0564 0.9094 0.0126 0.2683 A (8-21 cm) 0.0083 0.1034 0.6941 0.0403 0.6156 E 0.9536 0.6706 0.5785 0.7623 0.6168 B 0.9805 0.1316 0.3168 0.5134 0.0456 Values italicized are significant at = 0.05. Table 4-4. Soil pH averages for incorporated a nd surface applied treatments 8 months after WTR application. Application Method A (0-8 cm) A (8-21 cm) E B Incorporated 4.89 a 4.73 a 4.89 a 5.98 a Surface Applied 4.71 b 4.43 b 4.93 a 6.18 a Treatments with similar letters show no significant difference for that column by WallerDuncan (p<0.05). 43
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Soil pH is important when applying Al-WTR s to agricultural land because if the pH drops below 4.0, Al becomes more soluble and toxi city implications become more serious. The target pH for bahiagrass in Flor ida is 5.5 (Mylavarapu et al., 2007) The WTR used in this study had positive effects on soil pH, s howing an increasing trend with WTR application, but the soil pH values even after WTR application suggest that liming of this soil should be recommended. There may have been a slight acidifying effect from the ammonium nitrate fertilizer (89.7 kg N ha-1) applied after each harvest. However all tr eatments, including the controls, received the same fertilization so the relative effect of WTR a pplication rate on soil pH can still be observed. The pH of WTRs from differe nt treatment plants can vary from 5.3 to 8.0 based on their chemical makeup, how the WTR was produced a nd the characteristics of the water being purified (Silveira et al., 2006, Codling et al., 2002, and Ippolito et al., 2003). Independent of the exact pH of the material, WTRs have not expr essed a strong effect on the soil pH in recent studies. Ippolito et al. (2003) l ooked at changes in pH when WT R was shaken with a potassium chloride solution. Subsamples were taken th roughout a 215 day study and results showed the initial pH of the WTR solution was 7.6 and incr eased to 8.15 after approximately 135 days. In another study, soil samples from the top 2.5 cm with WTR applied at rates up to 18 Mg ha-1 showed no increase in pH after f our months (Haustein et al., 2000). The pH of the WTR in this study was 5.8 and the pH of the control plots was 5.5. In the same study, a HiClay Alumina (HCA) amendment was compared to the WTR. Th e HCA had a pH of 3.4 and reduced the soil pH from 5.53 (control) to 5.27 at an application rate of 18 Mg ha-1. Codling et al. (2007) applied combinations of poultry litter a nd WTR at rates up to 11.2 Mg ha-1 and observed minimal changes in pH due to WTR application. 44
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Tillage may cause pH to be higher than if the soil was left unmanaged. Godsey et al. (2006) reported that over many years no-till plots of wheat, s oybean and sorghum had a lower average pH than conventionally tilled plots. No-tillage systems can increase the organic matter (OM) of the soil. Shertz (1994) showed an example in which a farm in Illinois had an increase in soil OM from 1.87% to 4.0% in fifteen years of no till. Higher soil OM levels can potentially lead to an increase in soil acidity (Coleman and Thomas, 1967) Carbon dioxide is produced when OM decomposes, which releases H+ when it reacts with water. Organic matter also contains carboxylic and phenolic groups which act as weak acids (Havlin et al., 2005). This acidifying action of soil OM has a minimal effect on the overall soil pH in mineral soils but can still be a factor. No-till systems also have highe r rates of water infiltration and thus leach cations such as Ca+ which can be replaced by H+, causing higher rates of so il acidity over time (Powers and McSorley, 2000). Water Extractable Phosphorus Water extractable P is the form of P most easil y lost to leaching and s ubsurface transport. Application method affected WEP concentratio ns in the A (8-21 cm) and E horizons while application rate influenced WEP concentrations in the A (0-8 cm), A (8-21 cm) and E horizons (Table 4-5). The interactions between WTR appl ication method and rate were not significant. Table 4-5. The p-values for the WEP data us ing ANOVA and general m ean contrast (single degree of freedom). Soil Horizon Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 A (0-8 cm) 0.5962 <.0001 0.9011 <.0001 0.0341 A (8-21 cm) 0.0319 0.0004 0.223 0.0001 0.5329 E 0.0066 0.0004 0.9758 <.0001 0.5543 B 0.9731 0.447 0.5473 0.616 0.2504 Values italicized are significant at = 0.05. 45
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Water extractable P concentrations are show n by horizon for the incorporated and surface applied treatments in Figs. 4-1 and 4-2, re spectively. Recall that 14 weeks after WTR was applied to the appropriate treatments, a blanket application of P as trip le superphosphate (TSP) was surface applied at a rate of 224 kg P ha-1 to all plots including the controls. Concentrations of WEP increased in the controls compared to the pretreatment concen trations due to this application of P. Thus, data ar e also presented in these figures for WEP concentrations prior to the application of P. 46
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0 1 2 3 4 50 5 10 15 20 25 30WEP of Incorporated Treatments (mg kg-1)Initial Control 35 Mg / ha 70 Mg / ha Soil Horizon A (0-8 cm) A (8-21 cm) E B Figure 4-1. The WEP concentrations of incorporated treatments by soil horizon. The dashed line represents the average WEP concentration of all plots from the 2006 sampling before amendment application. The other lines represent the WE P concentrations from the 2007 soil sampling after amendment application. 0 1 2 3 4 50 5 10 15 20 25 30WEP of Surface Applied Treatments (mg kg-1)Soil Horizon Initial Control 35 Mg / ha 70 Mg / ha A (0-8 cm A (8-21 c E B ) m) Figure 4-2. The WEP concentrations of surface applied treatments by soil horizon. The dashed line represents the average WEP concentr ation of all plots from the 2006 sampling before amendment application. The other lines represent the WEP concentrations from the 2007 soil sampling after amendment application. 47
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There was considerable movement of WEP downward in the soil profile in the control treatments four months after TSP application. Increases in WEP concentrations were observed in all horizons except the B horizon (Figs. 4-1 and 4-2). It is not possible to determine if the lack of response in the B horiz on was due to the P bonding strength of the B horizon or to the lack of movement to this depth. Application of WTR decreased the WEP concentrations relative to the control in all horizons except the B hor izon where there was no effect. Differences between the 35 and 70 Mg ha-1 WTR application rates were only significant in the A (0-8 cm) horizon (Table 4-5). The WEP concentrations in the incorporat ed treatments of the A (8-21 cm) and E horizons were greater than the surface applied treatments (Table 4-5) suggesting there may be a potential effect of tillage on P tr ansport in the soil. Tilling de stroys macropores which allow for more rapid water movement (Hill and Mannering, 1995). Thus, no-tillage systems have greater water movement which may result in faster P transport and may help explain the WEP concentrations between the incorporated and su rface applied treatments. This P may not be observable in the B horizon due to extensive bonding by Al in the spodic horizon. These results are similar to previous results reported in the literature. Codling et al. (2000) studied the effects of WTRs and iron rich residues (IRR) when added to poultry litter and soils. Each amendment was added to the litter a nd soils at rates up to 10% dry weight by mass. Samples were incubated for 7 weeks and subsamples taken at 2, 4 and 7 weeks. The litter and WTR combination showed a decrease in water so luble P (WSP) as WTR rate increased. The WTR reduced WSP more than the IRR. Reductions in WSP compared to the controls at the highest application rate were 87% and 42% for the fourth week for WTR and IRR, respectively. Similar results were shown when a WTR was added to three different soils at rates up to 5% dry 48
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weight by mass in a wheat growth chamber e xperiment (Codling et al., 2002). Soil WSP decreased as WTR rate increased for all thr ee soils and was reduced 98 to 99% at the 5% application rate when compared to the controls. Mehlich-1 Extractions Mehlich-1 P concentrations were affected by application method in the A (8-21 cm) horizon (Table 4-6). The distri bution of Mehlich-1 P in the soil profile for incorporated and surface applied WTR treatments is shown in Figs. 4-3 and 4-4, respectively. As noted earlier for WEP concentrations, the blanket ap plication of TSP is responsible for the considerable increases in Mehlich-1 P in 2007 compared to 2006. Table 4-6. The p-values for the Mehlich-1 data using ANOVA and general m ean contrast (single degree of freedom). Response Variable Soil Horizon Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 A (0-8 cm) 0.2794 0.4589 0.8492 0.2202 0.9433 A (8-21 cm) <.0001 0.0696 0.5926 0.0617 0.1762 E 0.0811 0.1607 0.8637 0.0616 0.8085 Mehlich-1 P B 0.1635 0.3327 0.286 0.4761 0.1217 A (0-8 cm) 0.0099 <.0001 0.1267 <.0001 0.0026 A (8-21 cm) 0.0005 <.0001 0.0174 <.0001 0.0076 E 0.4174 0.038 0.1199 0.0375 0.0825 Mehlich-1 Al B 0.7486 0.8271 0.6664 0.5454 0.9947 A (0-8 cm) 0.0742 0.1745 0.5916 0.1465 0.227 A (8-21 cm) 0.7233 0.8367 0.7284 0.6436 0.6983 E 0.5292 0.2455 0.613 0.114 0.5795 Mehlich-1 Fe B 0.6607 0.0669 0.9241 0.0557 0.146 Values italicized are significant at = 0.05. The only difference observed for Mehlich-1 P concentrations was in the A (8-21 cm) horizon where the WTR incorporated treatments had greater concentrations than the surface applied treatments (Table 4-7). This may be a result of the incorporati on process since tilling did not occur exactly at 8 cm where the A horiz on was split for two samples. Mehlich-1 P concentrations followed the same general trend fo r all treatments in both the incorporated and 49
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surface applied treatments (Figs. 4-3 and 4-4). This trend shows less Mehlich-P in the A (0-8 cm) horizon of the controls than WTR amended tr eatments but more Mehlich-1 P in the A (8-21 cm) and E horizons of the controls than WTR amended treatment s. This suggests P is being retained near the surface in the WTR treated so ils and not moving lower down the soil profile. This trend may be more significant with time as P will continually move downward in the controls. 50
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0.00 1.00 2.00 3.00 4.00 5.0001 02 03 04 05Mehlich-1 P of Incorporated Treatments (mg kg-1)Soil Horizon0 Initial Control 35 Mg / ha 70 Mg / ha A (0-8 cm) cm) A (8-21 E BFigure 4-3. Mehlich-1 P concentrations of incor porated treatments by soil horizon. The dashed line represents the average concentration of all plots from the 2006 sampling before amendment application. The other lines represent the WE P concentrations from the 2007 soil sampling after amendment application. Initial Control 570 Mg / ha 35 Mg / ha 4 2 3 0 1 Figure 4-4. Mehlich-1 P concentrations of su rface applied treatments by soil horizon. The dashed line represents the average concentrati on of all plots from the 2006 sampling before amendment application. The other lines represent the WEP concentrations from the 2007 soil sampling after amendment application. 0 10 20 30 40 50 Mehlich-1 P of Surface Applied Treatments (mg kg-1 ) Soil Horizon A (0-8 cm A (8-21 cm E B ) ) 51
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Rew (2006) did not observe changes in soil Mehlich-1 P before or after rainfall simulations in which an Al-WTR was applied at rates up to 2.5% dry weight by mass. The soil was impacted with heavy loads of manures and so had initially high P concentrations. Before the simulations began, some treatments had the WTR mixed throughout the top 10 or 20 cm and no Mehlich-1 P differences occurred du e to this application method. Mehlich-1 Al concentrations were higher in the A (0-8 cm) and A (8-21 cm) horizons when the WTR was incorporated compared to su rface applied (Table 4-6; Figs. 4-5 and 4-6). Application rate of WTR affected Mehlich-1 Al concentrations in the A (0-8 cm), A (8-21 cm) and E horizons. Additions of WTR did not change the soil Fe concentration due to the low Fe concentrations in the Al-WTR (Table 4-6). 52
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Initial Control 0.00 5.000 100 200 300 400 500Mehlich-1 Al of Incorporated Treatments (mg kg-1)35 Mg / ha 70 Mg / ha 1.00 2.00 3.00 4.00Soil Horizon A (0-8 cm) A (8-21 cm) E B Figure 4-5. Mehlich-1 Al concentr ations of incorporated treatments by soil horizon. The dashed line represents the average concentration of all plots from the 2006 sampling before amendment application. The other lines represent the WE P concentrations from the 2007 soil sampling after amendment application. 0.00 1.00 2.00 3.00 4.00 5.000 100 200 300 400 500Mehlich-1 Al of Surface Applied Treatments (mg kg-1)Soil Horizon Initial Control 35 Mg / ha 70 Mg / ha A (0-8 cm) A (8-21 cm) E B Figure 4-6. Mehlich-1 Al concen trations of surface applied tr eatments by soil horizon. The dashed line represents the average concentrati on of all plots from the 2006 sampling before amendment application. The other lines represent the WEP concentrations from the 2007 soil sampling after amendment application. 53
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Higher Al concentrations suggest a greater potential of the soil to retain P. It also results in a greater need to be aware of possible Al t oxicity to plants. These potential effects will be discussed in the forage sec tion of this chapter. Soil Phosphorus Storage Capacity The soil P storage capacity (SPSC) was estimated from the following calculation determined by Nair and Harris (2004): SPSC (mg P kg-1) = (0.15 PSR) (Al + Fe) x 31 where PSR = (P) / (Al + Fe) and al l elements are expressed as mmole s. The P, Al and Fe values were Mehlich-1 extractable con centrations. The 0.15 value is th e threshold for Florida soils suggested by Nair and Harris (2004) and 31 is the atomic mass of P. When SPSC values are greater than 0 the soil is acting as a P sink and when SPSC values ar e less than 0 the soil is acting as a P source. The true SPSC calculation uses oxalate extractable con centrations instead of Mehlich-1 concentrations. Therefore the SPSC va lues shown here do not give exact values but are instead approximations in which relative cha nges can still be analyzed. There have been other instances when Mehlich-1 values for P, Al and Fe were used to calculate SPSC values (Nair and Harris, 2004). The SPSC factor is unique in that it takes into account not only how much P the soil contains from past loadings but also how much P retention capacity remains in the soil. Soil test P (STP) concentrations are often used to determ ine environmental risks due to P, but a low STP does not necessarily indicate that the soil has the capacity to retain added P (Nair and Harris, 2004). The average SPSC values prior to application of any treatment for the A (0-8 cm), A (821 cm), E and B horizons were 2, 4, 3 and 44 mg kg-1 respectively. The B horizon showed a higher SPSC value due to higher concentrations of Fe and Al. All horizons showed positive 54
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values suggesting the soils co uld retain additional P. The A and E horizons had low SPSC values close to zero, or equilibrium, implying th e soils could easily become P sources. Even though these soils had low initial P concentrations, they also contai ned very little Al and Fe to give them any significant P retention capacity. The SPSC was affected by the addition of P and WTR. Application method affected SPSC only in the A (0-8 cm) horizon while rate affected SPSC in all horizons except the B horizon (Table 4-7). Both of the control treatm ents became significantly negative as a result of adding P to a soil which had an initially low SPSC (F igs. 4-7 and 4-8). These soils have lost the ability to retain P and prevent leaching. SPSC values increased as WTR application rates increased at every horizon except the B horizon where P and Al levels were unaffected. Table 4-7. The p-values for the SPSC data using ANOVA and general mean contrast (single degree of freedom). Soil Horizon Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 A (0-8 cm) 0.0004 <.0001 0.019 <.0001 0.0006 A (8-21 cm) 0.1036 0.0012 0.1043 0.0003 0.5023 E 0.2506 0.018 0.7193 0.0065 0.4346 B 0.4223 0.7748 0.6761 0.4874 0.9717 Values italicized are significant at = 0.05. In the A horizon (0-8 cm), the incorporated treatments with WTR have greater SPSC values than the original soils suggesting that the applied WTR increased the soils ability to retain P even after a large amount of P was added. However, Fi gure 4-8 shows that the surface applied treatments with WTR were only slightly positive at the 70 Mg ha-1 application rate and dropped below zero at the 35 Mg ha-1 application rate. The E horizon soils treated with WTR continue d to have SPSC values near zero similar to the pre-amendment soils. While the WTR did not increase the soils capacity to retain P at 55
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this depth, it did prevent the soil from beco ming a P source as was observed in the negative values of the controls. -40-30-20-10010203040506070 4. 00 3. 00 2. 00 1. 00Soil P Storage Capacity of Incorporated Treatments (mg kg-1) Initial Control 35 Mg / ha 70 Mg / ha A (0-8 cm) A (8-21 cm) E B Figure 4-7. Soil P storage capacity of incorporated treatments by soil horizon. The initial values are the average SPSC values of all plots from the 2006 sampling before amendment application. The control and WTR amended values are from the 2007 soil sampling after amendment application. -40-30-20-10010203040506070 1. 00 2. 00 3. 00 4. 00Soil P Storage Capacity of Surface Applied Treatments (mg kg-1) Initial Control 35 Mg / ha 70 Mg / ha E A (0-8 cm) A (8-21 cm) B Figure 4-8. Soil P storage capacity of surface applied treatments by soil horizon. The initial values are the average SPSC values of all plots from the 2006 sampling before amendment application. The control a nd WTR amended values are from the 2007 soil sampling after amendment application. 56
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Figure 4-9 helps explain the differences in SPSC values of the incorporated and surface applied treatments. The incorporated treatmen ts had Al (from the WTR) mixed throughout the top 10-15 cm of the soil profile by the tilling pr ocess. The A horizon soil samples were taken above and below 8 cm. Samples were not mixed before being collected but rather taken at regular increments along the length of the profil e sampled (8 cm in this case) so the samples from the surface applied treatments contained soil that did not include WTR. Recall that the soil before WTR amendment had very low amounts of Fe and Al. Since this poor P retaining soil, coupled with a larger amount of P from TSP ap plication, was used to calculate the SPSC, the value dropped below zero even though WTR was present. The soil sampling process involved taking intact cores and placing them in sequence in a rain gutter. Subsequent subsampling from each increment was done by compositing random samples taken with a spatula. This subsampling protocol may have resulted in a non-uniform sampling of the given soil horizon thereby explaining differences in SPSC between incorporated and surface applied treatments. 57
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Al from WTR Incorporated TreatmentsSamples taken at 8 cm Al from WTR Surface Applied TreatmentsSamples taken at 8 cm Figure 4-9. Differences in SPSC for incorpor ated and surface applied treatments. Oladeji et al. (2006) also observed SPSC va lues in a bahiagrass field where WTR was surface applied at rates up to 2.5% dry weight by mass. Various P sources were added at N and P agronomic rates. It was found that independe nt of the P source or rate, SPSC increased as WTR rates increased. The SPSC values in this study were calculated using oxalate extractable concentrations. When P was app lied with TSP at values greater than plant requirements which were meant to mimic manure applications (88 kg P ha-1), the SPSC values were approximately 20 mg kg-1. In comparison, the SPSC values determined from Mehlich-1 concentrations in our study showed values with an average of 6 mg kg-1 (3% dry weight WTR, surface applied and under high TSP additions). Considering some di fferences existed between the two studies, SPSC 58
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values did not greatly vary and showed similar trends. Thus, the Mehlich-1 extractable concentrations used can give a good indication of the SPSC values. Soil Nitrification Potential Since the microbial nitrification process in so ils is sensitive to a variety of environmental factors, it was used as an indicator to evaluate any adverse effect that the Al-WTR might have on the soil microbial population. The assay used a solution of (NH4)2SO4 buffered at pH 7.2. Preliminary studies showed esse ntially no nitrate production when this test was first used. Because the soil had a relatively low initial pH (4.6), it was hypothesized that the buffered (NH4)2SO4 solution added to the soil did not have enough buffering capacity to overcome the low soil pH and the pH of the solution dropped to levels where microorganisms could not reach their optimum nitrification potential. This was c onfirmed by a subsequent test that showed the initial solution pH of 7.2 drop to 5.2 after addition of the soil. The pH of the soil was considered to be a limiting factor in this study so the a ssay was rerun with newly collected soil and the added solution contained a highe r concentration of phosphate bu ffer to prevent the pH from dropping. After increasing the buffer strength of the soluti on, nitrification potenti al increased as the application rate of WTR increased, especially in the surface applied trea tments (Table 4-8 and Fig. 4-10). The interactions betw een WTR application method and ra te were not significant. These results may be indicativ e of some of the possible be nefits of WTR application Ippolito et al. (2003) observed, incl uding increased N and organic carbon availability and greater aggregate stability. Potential nitrification rate s were lower in the incorporated treatments because the WTR was spread throughout the top 10-15 cm while the soil samples for this study were collected only from the top 5 cm. 59
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Table 4-8. The p-values for the nitrificati on potential data using ANOVA and general mean contrast (single degree of freedom). Sampling Date Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 September 20, 2007 <.0001 0.0504 0.1386 0.1687 0.0369 Values italicized are significant at = 0.05. 0 5 10 15 20IncorporatedIncorporatedIncorporatedSurfaceSurfaceSurface 0 Mg / ha35 Mg / ha70 Mg / ha0 Mg / ha35 Mg / ha70 Mg / ha Application Method and Ratemg NO3 --N kg-1 h-1 a b b c c c Figure 4-10. Nitrification potenti al of treatments for soil samples taken on September 20, 2007. Treatments with similar letters show no significant difference by Waller-Duncan (p<0.05). It is important to note that the values determined by this study are not actual field nitrification rates but rather potential nitrificat ion values. Factors whic h regulate nitrification rates such as temperature, NH4 + supply and pH were controlled for optimal results. In a study by Pecku et al. (2006), the effect s of WTRs on soil respiration and microbial community structure were evaluated. Six different WTRs were added to two soils at rates up to 500 Mg ha-1. Soil microbial respiration increased as WTR application rate increased. This increase was influenced not only by the applica tion rate but also by th e type of WTR and its chemical characteristics. Respiration rates d ecreased with time (10 days) indicating WTR may not enhance microbial activity for extended periods. Two WTRs had lo wer respiration rates when combined with the soils. The lower rates were considered to be a result of the acidic 60
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conditions present in the soil (pH < 5.2 and 6.2). Microbial community st ructure was analyzed with denaturing gradient gel elect rophoresis and diversity was not found to be adversely affected by WTR application. This study conc luded that WTRs could be applie d to these specific soils at rates up to 300 Mg ha-1 without negatively impacting the microbial community. In another study highlighting the effects of Al-WTRs on plant decomposition, C-labeled soybean leaves and stems were added to two so ils amended with WTR at rates up to 1% dry weight by mass. The Al-WTR did not affect deco mposition of either the plant material or soil organic matter. In comparison, an iron rich re sidue (not Fe-WTR) decr eased decomposition rate, likely because of pH or salinity changes caused by the amendment (Sikora, 2004). Both of the above studies showed similar re sults as found in our study: Al-WTRs did not cause adverse effects on the biological functions of soil as measured by soil microbial functions. These two studies were performed under labo ratory conditions while the Ona study employed soil and WTR samples taken from field conditions. Soluble Reactive Phosphorus in Groundwater Because of drought conditions during th e study period, wells c ontained sufficient groundwater for sampling only during a period fro m mid-September to mid-October (Figure 411). Samples were obtained at two dates duri ng this period: September 20, 2007, and October 2, 2007. 61
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00 9/ 14/07; 1 8: 18 0 9 /16/07;00:18 0 9/17 / 07;06:18 0 9/ 18/07; 12 : 18 0 9 /19/07;18: 1 8 0 9/21 / 07;00:18 0 9/ 22/ 0 7; 06 : 18 0 9 /23/07; 1 2: 18 0 9/24 / 07;18:18 0 9/ 26/ 0 7; 00:18 0 9/ 27/07; 0 6: 18 0 9 /28/07;12:18 0 9/2 9/ 07;18:18 1 0/ 01/07; 0 0: 18 1 0 /02Date 10 20 30 40 50 60 70/07;06: 1 8Depth Below Surface (cm) 9-14-07 9-16-07 9-18-07 9-20-07 9-22-07 9-24-07 9-26-07 9-28-07 9-30-07 10-2-07 Figure 4-11. Water depth data from the pressure transducer. The ground surface is located at zero on the y axis. The two sampling dates ar e circled and show they were collected recently after rain events. Many factors need to be considered wh en analyzing groundwater soluble reactive P (SRP) concentrations. The water table from wh ich the samples were collected was above the spodic horizon. Nair et al. (1999) showed that lateral moveme nt above the spodic horizon is possible and it cannot be ruled ou t under the field conditions pres ent in this study. Since SRP concentrations did not change between the tw o sampling dates (Fig. 4-12) and there was a difference due to WTR application in the surface applied treatments (Table 4-9), it is believed that subsurface lateral flow did not cause substantial variations in groundwater SRP concentrations. When the SRP concentrations we re looked at on a plot ma p there were no trends seen relating concentrations with subsurface mo vement. Sampling quickly after rain events may have helped to prevent subsurf ace lateral flow effects from ob structing the results from WTR application. 62
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Triple superphosphate was added in April and groundwater samples were not collected until September. The rate of P movement between these time frames is unknown. The study procedure represents a situation in which high loads of P would be applied after WTR application. If the soil had b een initially loaded with P th roughout the upper soil profile, groundwater data may have given different results especially visible in the incorporated treatments. Groundwater SRP concentrations were reduced by the surface application of WTR but were not affected when the WTR was incorporated (Figure 4-12). The standard deviation of the control surface treatment was very large in the first sampling but decreased considerably in the second sampling while not having different SRP concentrations. The second sampling gave a more stabilized and representative value of the SRP concentrations in these control treatments and was still higher than treatments with WTR. There were no differences between the two WTR application rates. Table 4-9. The p-values for the groundwater SR P data using ANOVA and general mean contrast (single degree of freedom). Sampling Date Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 September 20, 2007 0.5805 0.0695 0.104 0.0301 0.504 October 2, 2007 0.8413 0.026 0.0305 0.0123 0.336 Values italicized are significant at = 0.05. 63
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0 2 4 6 8 10 12IncorporatedIncorporatedIncorporatedSurfaceSurfaceSurface 0 Mg / ha 35 Mg / ha70 Mg / ha0 Mg / ha 35 Mg / ha70 Mg / ha Application Method and RateSoluble Reactive P (mg L-1) Sept. 20 Oct. 2 A a AB b AB ab B b B b B b Figure 4-12. Water SRP concentr ations across treatments fo r two sampling dates in 2007. Treatments with similar letters and casing s how no significant diffe rence by Waller-Duncan (p<0.05). The process of incorporation impacted the groundwater SRP concentrations. The surface applied control treatments had almost twice as high SRP concentrations than all of the incorporated treatments, including the incorp orated control treatments. As previously mentioned, tilling destroys macropores so water movement is greater th rough no-till systems. Shipitalo et al. (2000) studied th e leaching of anions and cations in tilled and no-till systems by using strontium bromide hexahydrate (SrBr2 6H2O) as a tracer. The Brwas leached more quickly in the no-till systems initially but overa ll anion loss after 2 years was not different between tilled and no-till systems. Thus ther e may have been greater leaching of SRP in the surface applied treatments, possibly explaining why the surface applied control had greater concentrations than the incorporated control. The SRP concentrations for the incorporated treatments showed no differences. The effects due to tilling seem to have prevailed over any possible effects resulting from WTR application that were seen in the surface appl ied treatments. Howeve r, the conditions brought 64
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about by tilling may only be temporary. Futu re sampling may be needed to show how groundwater SRP is affected by WTR application. The incorporated treatments with WTR did not have lower SRP concentrations than the surface applied treatments with WTR. This is likely because the main P source was applied only at the surface and was not present throughout the soil profile. Silveira et al. (2006) showed that by in corporating WTR in th e top 15 cm of manure impacted soil, SRP concentrations in leachat e were reduced more than if the WTR was incorporated in the top 7.5 cm. Control samples had SRP concentrations of 38 mg L-1, while the 7.5 and 15 cm incorporated samples had SRP concentrations of 19 and 2 mg L-1 respectively. Rew (2006) observed that WTR applied on the surf ace better controlled P in runoff, but that incorporating WTR better controlled P in subsurf ace flow and leachate concentrations. Both of these studies dealt with highly impacted soils which contained large am ounts of P throughout the profile due to excessive loads of manure, while th e soils at the Ona study s ite were naturally low in P and had a P source applied to the surface. Forage Yield and Nutritive Value The P sorbing characteristics of Al-WTRs help prevent P loss from leaching and runoff but also raise concern for P availability to plants and potential Al toxicity effects. Thus, forage yield and several forage quality parameters were closely observed to evalua te the effects of WTR application on bahiagrass. Forage Yield Forage was harvested in January 2007 prior to application of any treatment to evaluate uniformity of the study area. No differences acro ss treatments in forage yield were observed before WTR was applied. Yield averaged 5.8 tons ha-1 (Table 4-11). 65
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Table 4-10. The p-values for the forage yiel d data using ANOVA and general mean contrast (single degree of freedom). Month Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 January 0.9283 0.3292 0.4687 0.1426 0.9874 May <.0001 0.2742 0.4541 0.136 0.5674 June <.0001 0.1417 0.9699 0.077 0.3685 July 0.089 0.6099 0.6195 0.3596 0.7245 August 0.03 0.0799 0.0578 0.1147 0.0936 September 0.9674 0.3422 0.0845 0.3745 0.2444 Values italicized are significant at = 0.05. January data was collected before any amen dments were applied. May through September data was collected after WTR and TSP application. Treatment effects were evalua ted on five harvests taken dur ing May through September, 2007. The incorporation process caused yield reduction for the first two harvests (May and June) because the grass was not completely rees tablished from tilling (Table 4-11). In the subsequent harvests, there were no yield differences observed ex cept for August when yield was greater in the incorporated than in the surface applied treatments. Differences did not occur in the previous and following months so the August forage yield differences may be an anomaly. However, it may take additional yield measurem ents in the following year to confirm this. Table 4-11. Forage yield averages for incorp orated and surface applied treatments. Application Method January May June July August September ----------------------------------------Tons ha-1-----------------------------------Incorporated 5.77 a 0.05 b 0.45 b 2.62 a 4.27 a 2.25 a Surface Applied 5.74 a 0.41 a 1.44 a 2.91 a 3.90 b 2.25 a Treatments with similar letters show no differen ce for that column by Waller-Duncan (p<0.05). In this field study, WTR application rates up to 3% dry weight by mass did not indicate any trends toward negatively impacting bahi agrass yield. Oladeji et al. (2006) evaluated bahiagrass yields in a glasshouse pot experiment. An Al-WTR wa s applied on a dry weight basis at rates of 0, 1 and 2.5% dry weight by mass to a P deficient soil fert ilized at N and P based rates. Bahiagrass yields were reduced in the first harvest likely due to germination difficulties. In the second crop, yields were not aff ected at any WTR application ra te. In a study using stargrass 66
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grown in a P impacted soil with WTR at rates of 2.5% dry weight by mass, no effects on yield were observed (Rew, 2006). In a study by Codling et al. (2007), corn grai n yields were not adversely affected when poultry litter was mixed with an Al-W TR at rates of 0, 5.6 and 11.2 Mg ha-1 and applied to plots before corn was planted. However, wheat bioma ss yields were impacted at certain WTR rates in a study by Codling et al. (2002). Three soils of va rious textures including a silt loam, fine sandy loam and sand were mixed with WTR at rates of 0, 1, 2.5 and 5% dry weight by mass. Three cycles of wheat were grown and analyzed in a greenhouse pot experiment. The fine sandy loam and sand soils showed yield redu ctions at the 2.5 and 5% appli cation rates but no effects were found for the silt loam soil. Neutral Detergent Fiber Neutral detergent fiber (NDF) is often used as a forage quality indica tor and measures the amount of hemicellulose, cellulose and lignin in the forage. Th ese polymers are not directly composed of P, the element of concern for plan t availability with WTR application. However, observing no effect on NDF percentage does sugge st that WTR application does not interfere with reactions in the plant. Table 4-12. The p-values for the forage NDF data using ANOVA and general mean contrast (single degree of freedom). Month Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 January 0.9063 0.2741 0.1166 0.2185 0.2953 May 0.0553 0.6835 0.6908 0.4498 0.6787 June 0.0014 0.2438 0.6354 0.1529 0.3743 July 0.7035 0.9367 0.2993 0.8907 0.7426 August 0.0311 0.6239 0.8157 0.7243 0.3726 September 0.3016 0.7375 0.3985 0.7636 0.4787 Values italicized are significant at = 0.05. January data was collected before any amen dments were applied. May through September data was collected after WTR and TSP application. 67
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Neutral detergent fiber percentages did not ch ange due to WTR application rate over the course of the study (Table 4-12). Values were on ly affected by application method in the June and August harvests. In June, the surface applie d treatments had higher NDF percentages than the incorporated treatments. However, the control was not different than the treatments receiving Al-WTR suggesting that WTR was not a factor in causi ng the decrease in NDF. The August NDF averages for incorporated and surface applied treatments differed less than 1%, and were not different when analyzed using the Walle r-Duncan test (Table 4-13). Overall, NDF was not impacted by WTR application rate. Table 4-13. Forage NDF percentage averages for in corporated and surface applied treatments. Application Method January May June July August September ------------------------------Neutral Detergent Fi ber %--------------------------Incorporated 76.18 a 68.85 a 68.15 b 70.23 a 70.03 a 73.95 a Surface Applied 76.14 a 67.58 a 70.84 a 70.63 a 70.68 a 74.84 a Treatments with similar letters show no differen ce for that column by Waller-Duncan (p<0.05). The NDF percentages found in this study were similar to those found for bahiagrass in other studies in Florida. A study examining bahiagrass NDF ba sed on organic matter percentage at the RCREC in Ona, FL, showed values of 76% for both 4 and 10 week maturity periods (Arthington and Brown, 2005). Samples of co astal bermudagrass taken in October and November from dairies in the Suwanee River Va lley showed NDF content ranging from 78 to 85% (Staples et al., 2003). Crude Protein There were no pretreatment (January) differences among treatments in bahiagrass crude protein percentage (Tables 4-14). There were also no differences in crude protein content of forage due to WTR application rate in post-trea tment harvests. There was an effect due to application method as surface applied treatments had higher percentages than the incorporated treatments in May; in June and July the incorp orated treatments had higher percentages than the 68
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surface applied treatments. In general, less mature grasses have higher crude protein levels than more mature grasses (Arthington and Brown, 2005). While values were lower in May, the less mature grasses on the incorporated treatments almost doubled their crude protein content by June, possibly explaining why values were highe r than in the surface applied treatments. Table 4-14. The p-values for the forage crude protein data using ANOVA and general mean contrast (single degree of freedom). Month Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 January 0.5604 0.4889 0.1951 0.4049 0.3948 May 0.0001 0.8384 0.9865 0.8705 0.5747 June <.0001 0.4749 0.6777 0.2871 0.5645 July 0.0058 0.982 0.5071 0.8514 1 August 0.0949 0.8471 0.7832 0.8514 0.5924 September 0.4913 0.1407 0.2947 0.0514 1 Values italicized are significant at = 0.05. January data was collected before any amen dments were applied. May through September data was collected after WTR and TSP application. Table 4-15. Crude protein percentage averages for incorporated and surface applied treatments. Application Method January May June July August September ----------------------------------Crude Protein %------------------------------Incorporated 6.10 a 8.28 b 15.25 a 12.42 a 10.19 a 13.19 a Surface Applied 6.30 a 9.85 a 13.58 b 11.13 b 9.82 a 13.06 a Treatments with similar letters show no differen ce for that column by Waller-Duncan (p<0.05). The crude protein percentage of bahiag rass grown in the summer ranged from 6.8 to 10.3%. Percentages dropped as maturity times in creased from 4 to 6 weeks and 6 to 8 weeks (Sollenberger et al., 2006). Thes e values were lower than the ba hiagrass values seen in our study but may be indicative of an unfertilized system. Tissue Nutrient Effects Phosphorus The pretreatment forage harvest showed no di fferences in P tissue concentration (0.12 0.15%) across all plots. Pr etreatment concentrations were ve ry low because of the initial low soil P concentrations (See Table 4-1). Tissue P concentrations were affected by application 69
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method for all harvests (Tables 4-16). Su rface applied WTR resulted in lower tissue P concentrations than when WTR was incorporated. The surface applied treatments have nearly all of the applied WTR still present close to the gr ound surface where most of the bahiagrass roots are located. This higher concen tration of WTR may be adsorbing P that would otherwise be available for plant uptake. Plant maturity may al so partially explain why the less mature plants of the incorporated treatments showed higher tissue P. Less mature plants take up nutrients at a quicker rate than more mature plants. For in stance, in sorghum when half the plant mass had been produced 60% of P, 70% of N and 80% of K had already been taken up (Vanderlip, 1993). The surface applied controls may have been aff ected by the unusually large amount of P applied and thus were not lower than the incorporated co ntrols of less maturity. Application rate was also significant at each harvest. However, th ere were differences between the 35 and 70 Mg ha-1 rates for only the July and August harvest dates. Table 4-16. The p-values for the forage tissue P data using ANOVA and ge neral mean contrast (single degree of freedom). Month Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 January 0.4676 0.7058 0.4934 0.5668 0.5519 May 0.0123 <.0001 0.0743 <.0001 0.1707 June 0.0032 0.0002 0.8144 <.0001 0.1113 July 0.0029 <.0001 0.0194 <.0001 0.0156 August 0.014 <.0001 0.0004 <.0001 0.0098 September 0.0208 <.0001 0.039 <.0001 0.1068 Values italicized are significant at = 0.05. January data was collected before any amen dments were applied. May through September data was collected after WTR and TSP application. 70
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Table 4-17. Tissue P content. WT R application rate in Mg ha-1 is represented by 0, 35 and 70. Application treatment is repres ented by I (incorporation) and S (surface application). January May June July August September Treatment ---------------------------------Tissue Phosphorus %---------------------------------0 I 0.14 a 0.86 a 0.90 a 0.48 a 0.42 ab 0.43 a 35 I 0.13 a 0.57 b 0.63 b 0.36 ab 0.32 b 0.32 b 70 I 0.15 a 0.52 b 0.46 c 0.31 bc 0.26 c 0.29 bc 0 S 0.14 a 0.89 a 0.71 b 0.50 a 0.49 a 0.46 a 35 S 0.12 a 0.37 c 0.33 cd 0.26 bc 0.22 0.24 cd 70 S 0.12 a 0.21 d 0.22 d 0.19 c 0.17 0.20 d Treatments with similar letters show no differen ce for that column by Waller-Duncan (p<0.05). Tissue P concentrations were highest dur ing May and June and then decreased to relatively stable concentrations for the remainder of the harvests. The decreased P concentration for July and August may be due to the higher yields during this time resulting in dilution of the tissue P (Table 4-11). In additi on, P concentrations in the soil solution may have decreased due to leaching as 25 cm of rainfall occurred after TSP application (FAWN). The treatment most impacted as measured by tissue P% was the surface applied 70 Mg ha-1 treatment. Tissue P concentrations were low and rela tively constant for this treatme nt throughout the entire study and ranged from 0.17 to 0.22%. The August average of 0.17% was just slightly above the limiting value of tissue P concentration of 0.15% (Mylavarapu et al., 2007). Oladeji et al. (2006) observed decreasing plant P tissue concentrations with WTR application rates at 1 and 2.5% dr y weight by mass when fertilizer s (biosolids, manures and TSP) were applied at P agronomic rates of 44 kg P ha-1. However, when ferti lizers were applied at N agronomic rates (P application ranged from 88-280 kg P ha-1 depending on the N:P ratio in the source), treatments amended with WTR had higher ti ssue P concentrations than the controls at P agronomic rates. This suggests that under N agr onomic rates, WTRs do not cause the tissue P to drop below critical values even though tissue P is lowered. These results are similar to our study where P was applied at high rates (224 kg P ha-1) to a WTR amended soil and tissue P levels 71
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decreased but did not fall below th e critical value. Rew (2006) observed no differences in shoot P of stargrass when WTR was surface applied or mixed in the top 10 cm and 20 cm. Stargrass was planted in boxes after the WTR and soil were mixed. In a study by Codling et al. (2007) with WT Rs, tissue P levels for corn grain and ear leaves stayed above the minimum level needed for adequate growth (2 g kg-1) at WTR rates of 0, 5.6 and 11.2 Mg ha-1. Wheat showed deficient amounts of P (< 1.5 g kg-1) when WTR was applied at rates of 2.5 and 5% dry weight by mass. The wheat in this study was grown in pots so deficiencies may have been exacerb ated because roots may have been constricted in the pots. In the field, roots may have grown to depths below WTR application to obtain adequate P for plant tissue. Aluminum Tissue Al for January, May and June showed concentrations below 370 g g-1 (data not shown) which is within normal levels (50 and 3410 g g-1) as reported by Kabata-Pendias and Pendias (2001). Concentrations did not increas e with WTR application rate and no differences occurred between treatments. Forage samples fo r July through September were analyzed with a different instrument than the January, May and June samples because the original instrument failed and could not be repaired in time to analy ze the later samples. The substitute instrument had a detection limit of 1.0 mg L-1, and the July through September samples had Al tissue concentrations below this limit. While Al tissue concentrations do not appear to have been affected by WTR application, it is unusual for Al to be transported from the root s to the leaves so low Al concentrations in the forage may not be indicative of signs of Al stress or toxicity. Cation uptake by roots is restricted when Al toxicity is affecting plants. Thus, cal cium (Ca) and magnesium (Mg) levels were also evaluated. 72
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Calcium and Magnesium Table 4-18. The p-values for the forage tissue Ca and Mg data using ANOVA and general mean contrast (single degree of freedom). Nutrient Month Method Rate Method*Rate Control vs. Treatments 35 Mg ha-1 vs. 70 Mg ha-1 July 0.9331 0.0185 0.7056 0.0159 0.0958 August 0.0015 0.2582 0.7376 0.1923 0.31 Calcium September 0.005 0.0472 0.5771 0.0153 0.816 July 0.6282 0.7173 0.1722 0.5842 0.5539 August 0.0657 0.0304 0.0668 0.0163 0.2267 Magnesium September 0.6684 0.8677 0.1439 0.6497 0.7927 Values italicized are significant at = 0.05. July through September data was collected after WTR and TSP application. Tissue Ca concentrations for July through September samples showed little variation ranging from 0.34% to 0.40% (Table 4-19) but did experience some effects due to application method (August and September) and rate (July an d September) (Table 4-18). Surface applied WTR treatments had slightly higher tissue Ca concentrations than incorporated treatments. Treatments amended with WTR had higher tissue Ca concentrations. Decreased Ca uptake was not observed due to WTR application. Magnesium concentration was only affected in August by application rate (Table 4-18). The surface app lied control treatment was higher than all other treatments and may be an anomaly (Table 4-20 ). It does not appear cation uptake is being negatively affected by WTR application rate but may need to be monitored in the future. Table 4-19. Tissue Ca percentages for July through September. July August September Treatment ----------------------------Tissue Ca %---------------------------0 I 0.353 a 0.353 c 0.333 b 35 I 0.360 a 0.355 bc 0.365 ab 70 I 0.385 a 0.373 abc 0.368 a 0 S 0.343 a 0.383 abc 0.373 a 35 S 0.370 a 0.393 ab 0.392 a 70 S 0.388 a 0.395 a 0.385 a Treatments with similar letters show no differen ce for that column by Waller-Duncan (p<0.05). 73
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Table 4-20. Tissue Mg percentages fo r July through September. Application Rate July August September ----------------------------Tissue Mg %---------------------------0 Mg ha-1 0.32 a 0.35 a 0.28 a 35 Mg ha-1 0.30 a 0.33 b 0.28 a 70 Mg ha-1 0.32 a 0.32 b 0.27 a Treatments with similar letters show no differen ce for that column by Waller-Duncan (p<0.05). Codling et al. (2007) saw no changes in leaf or grain concentrations of Al, Ca or Mg at WTR rates up to 16.8 Mg ha-1. Other minerals were also studied, including zinc, manganese, iron and potassium. Similarly, there were no impacts from WTRs on these minerals. Rew (2006) monitored stargrass tissu e Al concentrations below 40 g g-1 in a rainfall simulation experiment with WTR application rates up to 2.5% dry weight by ma ss. The Al concentration of roots were also examined and showed no increa ses due to WTR. The Ca and Mg content in forage averaged over flatwoods and fresh ma rsh sites in South Florida was 0.19% and 0.09%, respectively (Kalmbacher and Ezenwa, 2006). 74
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CHAPTER 5 CONCLSUIONS The use of Al-WTRs has been proposed as a means to help reduce P runoff and leaching from agricultural land where soils are loaded with high amounts of P or have a low capacity to retain P. Many laboratory and greenhouse st udies have been conducted to evaluate the efficiency of WTRs to retain P and to ascertain th e potential for Al toxicity to plants. This field study evaluated the effects on soil P retenti on and movement when applying an Al-WTR followed by a heavy application of P to a soil with a low capacity to retain P. High amounts of P were added in the form of triple superphosphate (TSP) to simulate hi gh P loadings that may result from biosolid or manure application. An Al-WTR was added at 1.5 and 3.0% of the dry weight of soil to a bahiagrass pasture in South Florid a. Half of the plots we re disked in order to incorporate the amendment in the top 10-15 cm. Soil samples were taken from each horizon to a depth of one meter 8 months after WTR applicati on. Soil pH, water extractable P, Mehlich-1 P, Al and Fe, and the soil P stor age capacity (SPSC) were measure d. The soil microbial population was monitored with a nitrificati on assay. Groundwater samples were collected from wells placed above the spodic horizon in each plot. Forage samples were collected every 30 days for 5 months to monitor for toxicity effects. Yield, neutral detergen t fiber (NDF), crude protein and tissue analysis including P, Al, Ca and Mg content were observed. Soil pH increased slightly with WTR applic ation, thereby creati ng better conditions for bahiagrass growth and also decreasing the threat of Al toxicity. The addition of WTR reduced water extractable P concentrations in the A and E horizons. Mehlich-1 P concentrations showed no increases due to WTR application, but increase d from previous conditions only because of the large amount of P added as TSP. Mehlich-1 Al concentrations and SPSC increased as WTR application rate increased. The soils capacity to retain P increased even after heavy P loadings 75
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which should reduce P movement through the soil. Long term studies are needed to establish the continual benefits of WTRs on soil P retention. The soil microbi al population was not adversely affected as nitrification potentia ls increased as WTR application rate increased. The analysis of soluble reactive P (SRP) in groundwater showed a decrease in the surf ace applied treatments with WTR application but no changes among the inco rporated treatments. Tillage seems to have affected SRP concentrations but future sampling may show results without interference from the incorporating process. Regular groundwater sampling in the futu re may also help to validate current results. Forage yield, NDF and crude protein were not affected by WTR application rate for the duration of the study. Tissue analysis did not show additional uptake of Al and the uptake of Ca and Mg was not restricted, suggesting Al has not interfered with cation uptake by roots. Tissue P declined with added WTR but concentrations appear to have stabilized over the five harvests and did not drop below the critical level for bahiagrass. Again, future analysis is needed to assure tissue P does not drop any further. One of the issues associated with Al toxicity is the restriction of root growth. This study did not attempt to measure root biomass be cause this would have required complete removal of the bahiagrass on th e study site. The field plots were preserved so future research can be continued. Decreased r oot growth can result in less nutrient and water uptake, affecting growth and yield. This situation ha s the potential to develop with time so future sampling is needed to assure toxicity effects do not present themselves. Based on this study, the use of WTRs is reco mmended as an amendment to soils with low P retention capacity that have been heavily impa cted with P or will receive high amounts of P, e.g., a dairy sprayfield. The WTRs have shown to lower WEP concentrations below those of untreated soil. The SPSC values have also shown an increase, even with high loads of applied P, 76
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suggesting even more P can be retained if adde d to the soil. Incorporation of the WTR is recommended based on two main factors: bahiag rass tissue P concentration was less affected under these conditions and the SPSC benefited greater in the A horizon. However, the physical incorporation process is not al ways practical. If applying WTR to a large area, surface application may be easier and less destructive when considering extra time is needed for regrowth after disking. Surface appl ication is still very effective at P retention, especially when the P source is being applied after WTR application. No adverse effects were observed by forage yield or nutritive value at any rate. Therefore, the 70 Mg ha-1 application rate is recommended since it adds a greater amount of Al to the soil, further incr easing the capacity of the soil to retain P than lower application rates. Forage yield and tissue P concentrations need to be analyzed in the future to assure toxicities do not develop with time. The prevention of P loss from agricultural fi elds is essential for the health of aquatic systems. The application of Al-WTRs has the pote ntial to achieve these goa ls but the quality of the crop must also be seriously re garded. Different crops may have varying sensitivities to Al in the soil or to how much tissue P is required for normal plant gr owth and function. Application method and rate are important fact ors to consider when applying WT Rs. Future analysis of the soil and forage on this study site would be useful to assess long term implications of WTR usage. 77
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LIST OF REFERENCES Alexander, M. 1980. Effects of acid precipita tion on biochemical activiti es in soil. p. 47-52. In Proceedings of the Internati onal Conference on Ecological Imp act of Acid Precipitation, Sandefjord, Norway. SNSF Project, 47-52. Allen, L.H. 1987. Dairy-siting criteria and ot her options for wastewat er management on high water-table soils. Soil Crop Sci. Soc. of Fl. Proc. 47:108-127. Agyin-Birikorang, S., G.A. OConnor, L.W. Jacobs, K.C. Makr is, and S.R. Rinton. 2007. Long-term phosphorus immobilization by a drinking wa ter treatment residual. J. of Environ. Qual. 36:316-323. Ann, Y., K.R. Reddy, and J.J. Delfino. 1999. In fluence of chemical amendments on phosphorus retention in a construc ted wetland. Ecol. Eng. 14:213. Arthington, J.D. and W.F. Brown. 2005. Estimati on of feeding value of four tropical forage species at two stages of maturit y. J. of Anim. Sci. 83:1726-1731. Bitton, G. Volk, B.G., Graetz, D.A., Bossart, J.M., Boylan, R.A., and yers, G.E. 1985. Effect of acid precipitation on soil microbial activity: II. field studies. Journal of Environmental Quality 14:69-71. Board on Agriculture. 1980. Mineral Toleranc e of Domestic Animals. Subcommittee on mineral toxicity in animals, Committee on an imal nutrition. National Academy of Sciences, Washington, D.C. Botcher, A.B. and R.D. Rhue. 1984. Fertiliz er management key to a sound water quality program. EDIS. http://edi s.ifas.ufl.edu/CIR-816. Bollmann, Annette. 2006. Nitrif ication in soil. p. 136-179. In J. Bloem et al. (ed.) Microbiological methods for assessing soil qua lity. CABI Publishing, Cambridge, MA. Brady, N.C. and R.R. Weil. 2002. Elements of the Nature and Propertie s of Soils. Prentice Hall, Upper Saddle River, NJ. Burgora, B.A. 1989. Phosphorus spatial distri bution, sorption, and transport in a Spodosol. Ph.D. Dissertation. University of Florida. Chambliss, C.G. and M.B. Adjei. 2006. Bahiagrass. EDIS. http://edis.ifas.ufl.edu/SS-AGR-36. Codling, E. E., R. L. Chaney, and C. L. Mulchi. 2000. Use of aluminum a nd iron rich residue to immobilize phosphorus in poultry litter and litter amended soils. J. of Environ. Qual. 29:19241931. 78
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87 BIOGRAPHICAL SKETCH Julie Lauter Driscoll was born in 1984. She is the third daughter of Michael and Connie Driscoll and was born and raised in Fort Pierce, FL. Coming from a long line of Gators, she began her undergraduate degree in August 2002 at the University of Florida where she decided to major in soil and water science. She worked at the Indian River Research and Education Center in Fort Pierce for three summers where sh e completed an internship and an independent work study in a soil toxicology lab. It was thes e experiences where she discovered a desire to protect agriculture and the environment and decided to pursue her Master of Science in the summer of 2006. Julie worked to prevent phosphorus leaching from impacting surface and ground waters at a field study in Ona, FL.
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