Group Title: Added resolution elements in tandem mass spectrometry for chemical analysis /
Title: Added resolution elements in tandem mass spectrometry for chemical analysis
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 Material Information
Title: Added resolution elements in tandem mass spectrometry for chemical analysis
Physical Description: vii, 169 leaves : ill. ; 28 cm.
Language: English
Creator: Fetterolf, Dean Donald, 1956- ( Dissertant )
Yost, Richard A. ( Thesis advisor )
Dorsey, John G. ( Reviewer )
Winefordner, James D. ( Reviewer )
Eyler, John R. ( Reviewer )
Moye, Hugh A. ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1983
Copyright Date: 1983
Subjects / Keywords: Mass spectrometry   ( lcsh )
Chemistry, Analytic   ( lcsh )
Chemistry thesis Ph. D
Dissertations, Academic -- Chemistry -- UF
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
Abstract: The concepts of information theory have been applied to tandem mass spectrometry. The roles of the added resolution elements, which increase the informing power, were investigated for chemical analysis using a triple quadrupole mass spectrometer. It has been demonstrated that the added resolution elements in sample introduction and ionization as well as in the collisionally activated dissociation (CAD) process lead to increased sensitivity and selectivity for structure elucidation and mixture analysis . The effects of collision energy on the appearance of the daughter spectra were investigated in terms of energyresolved breakdown curves. These curves show good agreement with theoretical curves predicted by the quasiequilibrium theory (QET) and with those obtained by angle- resolved mass spectrometry. The effects of energy and pressure on the efficiency of the CAD process ivere also investigated. These resolution elements increase our understanding of the CAD process, but also aid in the differentiation of isomeric ion structures. For example, the cyclopropane ion can be differentiated from the linear propene ion by energy-resolved breakdown curves. The use of reaction collisions has been demonstrated to be a powerful new method for differentiating isomeric ion structures. The cyclopentadiene and the linear l-propene-3-yne molecular ions can be readily distinguished by reactive collisions xvith isobutane. These reactive collisions are also helpful in determining the mechanisms for the formation of adduct ions in chemical ionization, as well as those occurring in the reactions of hydrocarbon ions with acetylene. The careful selection and optimization of these added resolution elements in mixture analysis have lead to the development of a method for the rapid screening (100 samples/hr) of chlorinated compounds in serum and urine at the sub-ppb level. Limits of detection of 0.05 ppb and 0.250 ppb for hexachlorobenzene and trichlorophenol respectively, were obtained for a 1 yl injection of an unconcentrated extract of serum or urine. Combined chromatography and tandem mass spectrometry has been demonstrated for the separation of isomers and the identification of classes of compounds in complex environmental samples through the use of the various operating modes in MS/MS.
Thesis: Thesis (Ph. D.)--University of Florida, 1983.
Bibliography: Bibliography: leaves 163-168.
Statement of Responsibility: by Dean Donald Fetterolf.
General Note: Typescript.
General Note: Vita.
 Record Information
Bibliographic ID: UF00099352
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000366050
oclc - 10015281
notis - ACA4882


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