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Construction, Overexpression, and Preliminary Purification of a Plasmodium falciparum Plasmepsin 6 Prosegment Chimera

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Title:
Construction, Overexpression, and Preliminary Purification of a Plasmodium falciparum Plasmepsin 6 Prosegment Chimera
Creator:
Henry, Rachel
Dunn, Ben ( Mentor )
Place of Publication:
Gainesville, Fla.
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University of Florida
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Language:
English

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serial ( sobekcm )

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University of Florida
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University of Florida
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All applicable rights reserved by the source institution and holding location.

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Construction, Overexpression, and Preliminary Purification of a
Plasmodium falciparum Plasmepsin 6 Prosegment Chimera

Rachel Henry


ABSTRACT


Malaria is a parasitic infection that affects 300-500 million people annually. The Plasmodia parasites are

increasingly resistant to current drugs, and plasmepsins are essential hemoglobinases that are putative new

targets of Plasmodium falciparum. The structures of the plasmepsins reveal prosegment inactivation of the

enzyme by conferring a structural change in the protein and an alteration in the active site cleft. The enzyme

self-activates at acidic pH by removing the prosegment, leaving the mature enzyme in the proper conformation.



This project aims to study plasmepsin 6 (PM6), a recently discovered enzyme with amino acid sequence similarity

to plasmepsin 2 (PM2) and plasmepsin 1 (PM1). Prior attempts to overexpress PM6 with both a full length

and truncated prosegment have failed. It is hypothesized that if a protein shows poor levels of overexpression,

a different prosegment from a homologous protein may be used to mediate expression and refolding of the protein.



A chimeric protein comprised of the prosegment of PM2 joined to the mature PM6 sequence (pPM2gPM6) was

created through overlap PCR. Overexpression and refolding conditions were optimized, and purification is

currently underway. Preliminary assays indicate that pPM2gPM6 self-activates at acidic pH and activity was

observed by hydrolysis of aspartic proteinase substrates.



INTRODUCTION


Malaria is a parasitic infection that results in over 1.5 million deaths annually, and 300-500 million people

are currently infected with malaria worldwide (1). The parasite spends one part of its life cycle in female

Anopheles mosquitoes and the other part in humans (2). As the mosquito is feeding, sporozoites in the saliva

are transferred to the blood of the host. They travel through the blood and initially infect liver cells, in which

they develop into trophozoites. These trophozoites develop into merozoites, which erupt from the liver cells

and travel into the blood stream to invade erythrocytes. During this intraerythrocytic stage they develop

into trophozoites, and they degrade massive amounts of human hemoglobin as a nutrient source, which causes

the major presentation of disease in the human host (2,3). It is during this stage, from the acidic digestive





vacuole, that the first two plasmepsins were initially isolated (4).


One of the drugs used to treat malarial infections with minimal side effects is chloroquine. Chloroquine-

resistant Plasmodium falciparum strains emerged in Africa in 1978, and strains that are resistant to many of

the current therapeutics are widespread (5). This increasing resistance has made the development of new drugs

a necessity. One of the possibilities for new drug targets includes a class of enzymes called plasmepsins.

Plasmepsins are Plasmodia aspartic proteases that function in hemoglobin degradation during the

intraerythrocytic stage of the life cycle (6). Hemoglobin is an essential nutrient source, and various aspartic

protease inhibitors have been shown to kill intraerythrocytic cultures of P. falciparum (7,8,9).



Aspartic proteases exist both in an immature zymogen form, with the prosegment blocking the active site of

the enzyme, and an active form, in which the prosegment has been autocatalytically cleaved (10). Ten

plasmepsins have been identified to date, only a few of which have been characterized and studied (11). Attempts

to express full-length plasmepsin 6 have failed in the past, and a new method was needed to study this

enzyme. Prosegment chimeras are advantageous, because a different prosegment can be used to help mediate

the expression and refolding of the protein, but at a low pH, the prosegment gets cleaved off, leaving only

the desired enzyme behind.



MATERIALS AND METHODS


Background Primers were designed that were specific for the prosegment of PM2 (pPM2) and the gene segment

of PM6 (gPM6). Primer 2 had an overlapping region that was complementary to gPM6, and primer 3 had a region

that was complementary to pPM2. Primers 1 and 4 had BamHI cleavage sites incorporated into them. The pPM2

and gPM6 fragments were amplified through PCR and run on an agarose gel. They were excised from the gel and

gel purified. The fragments were allowed to extend at 72 oC for 10 minutes with Taq polymerase before the

primers (1 and 4) were added and amplified through overlap PCR (see Fig. 1). This PCR product was run on a

gel, gel purified, and ligated into a pCR.2.1 TOPO cloning vector (see Fig. 2). The vector was transformed

into TOP10F' One Shot E. coli cells, which were plated on LB/ampicillin plates. Colonies were plucked, cultures

were grown overnight, and plasmid DNA was isolated from them with a QIAprep Spin Miniprep kit. The clones

were screened with a BamHI restriction digest, and those that appeared correct were sent for verification

through sequence analysis.





Primer 1 Prinmr 3
____ _ PM2 proiegment
PM2 PM6
A1 1 -11 PM2 gene segment
PiriwrZ + PCR PCR Prinmer4progni
- ' PM6 prosegnent

i ^PM6 gene segment
- i 2BamH1 site
PM2 Proeegmenr PM6 Gene



p PM2 gPM6 Primer 1




Overlap PCR T
(extension, no primer)


Figure 1. pPM2gPMG Prosegment Chimera Construction





pCR' 2. TOPO-


















M� ftR -~



Figure 2. TOPOs.1 Cloning Vector



The TOPO vector plus insert and a pet3a vector were both digested with BamHI and run on a gel. The inserted

gene and the digested pet3a vector were excised from the gel, gel purified, and the insert was ligated into the

pet3a expression vector (see Fig. 3), and subsequently transformed into TOP10F' One Shot cells. Clones

were screened for directional insertion through a restriction digest with XbaI, and clones were verified

through sequence analysis. Plasmid DNA was isolated from a positive clone, and a transformation was done with

the DNA and BL21(DE3)pLysS supercompetent E. coli cells. Colonies were plucked from the plates and

grown overnight in LB media with ampicillin. Plasmid DNA was isolated and the clones were subsequently

digested with XbaI and run on a gel to screen for positives. Possible positive clones were verified through

sequence analysis, and cell stocks were made by adding 15% glycerol and storing at -20oC.





























Figure 3. pet3a Expression Vector



A 200mL culture of a positive clone was grown overnight with 200pL ampicillin, and was used to inoculate 4L of

fresh LB media. Once the cells had reached an OD at 600nm of 0.5-0.8 AU, 0.4mM IPTG was added to

induce overexpression of the plasmid protein. Samples and OD readings were taken at t=0, t=1, t=2, and t=3

hours. The OD readings were used to normalize the amount of protein loaded on the SDS-PAGE gel. After

three hours, the cells were spun down and the cell pellet was weighed and re-suspended in TE buffer. The

suspension was lysed through French Pressure Cell lysis and inclusion bodies, which are insoluble protein

aggregates, were purified by centrifugation with several different buffers. The resulting pellet was weighed after

the final step, and was re-suspended in TE buffer at 50mg/mL.



Inclusion bodies were solubilized at lmg/mL in 8M urea, 0.05M CAPS, 0.005M EDTA, and 0.2M BME. The

solubilized protein was put in dialysis tubing and was dialyzed against 50mM Tris pH 11.0 at room temperature

for four hours. Then the buffer was changed and allowed to sit overnight at 4>>C. The buffer was changed three

more times with 50mM Tris pH 8.0, 20mM Tris pH 8.0, and 20mM Tris pH 8.0, respectively, at twelve-hour

intervals. For the anion exchange column, the last three buffers were pH 9.5 instead of pH 8.0. Refolded protein in

a 20mM Tris pH 8.0 buffer was loaded onto a Superdex 75 gel filtration column, and fractions were tested for

activity and run on an SDS-PAGE gel. Protein in a pH 9.5 buffer was loaded onto a Sepharose High Performance

anion exchange column, and the fractions that eluted were run on an SDS-PAGE gel and tested for activity. For

the activity assays, the enzyme was incubated at 370C for 10 minutes in 0.5M sodium format buffer pH 4.5,

and then the substrate RS6P2V was added. The average absorbance change from 284-324 nm was monitored with

a spectrophotometer. A decrease in absorbance was indicative of substrate cleavage.









3.0kb
2.0kb
].5kb
1.Okb

0.5kb






i' \p Ct (41i It 5
1,\pt, ul( 1561 p)


t
gPN 16 fragment
(expected 1044bp)


Figure 4. PCR Amplification pPM2 and gPM6


3.0kb
2.0kb -
I .'5k'b

I.Okb~


0.5kb-~


I Expected 1200bp
(156+1044)


Figure 5. Overlap PCR Amplification of pPM2gPM6



RESULTS


The construction of the pPM2gPM6 chimera through overlap PCR was successful, as shown in Fig. 4 and 5, and
so was the overexpression and refolding, as show in Fig. 6 and 7, respectively. Gel filtration (Fig. 8) and
anoin exchange (Fig. 9) both helped in purification of the protein, but additional purification is still
necessary. Preliminary activity of the post-dialysate refoldedd protein) was seen,with 100pL of PD giving a
negative change in absorbance of 132x10-6 Absorbance Units/sec from 284-324 nm for the initial rate.





To1 T=2 T43 S1 S2 S3 84 IB


B1.OkDa . - . .

52.5kDa -

3SZkDaE 00 --



LB. OAmM IPTG, 37MC, IBL21l aE3)pI.%.SI
pPM2gPM6 calculated molecular weight ~46kDa


Figure 6. Expression of pPM2gPM6 in E.coli and Inclusion Body Purification


52.5kDa

36.2kDa


4- pFM2gPM6
45.IkDa


IB: Inclusion Bodies
PD: Post Dialyvate

Figure 7. Post-Dialysate (Refolded Protein)


MW 24 25 26 27 28 29 30


31 32


Figure 8. Superdex 75 GF Column Fractions


MW TWO
6~





30 32 34 37 38 39 40 MW


Figure 9. Sephorex Anion Exchange Column Fractions


DISCUSSION


The amplification of the segments was successful, as was the overlap PCR to join them. Overexpression and

refolding of pPM2gPM6 was successful, and in all of the publications to date, attempts to express PM6 have

failed. Preliminary activity was seen with the post-dialysate and the post-gel filtration column protein samples,

but the pPM2gPM6 enzyme that was used for the activity assays was in either a pH 8.0 or a pH 9.5 Tris buffer.

When the sodium format pH 4.5 buffer was added to drop the pH and induce activation of the enzyme, it

induced precipitation of the protein. This precipitation could be seen as opacity in the cuvette, and caused

significant scattering of light, which interfered with the assay.



A new refolding method is currently underway, which uses rapid dilution and then the addition of oxidized

glutathione instead of dialysis to promote refolding of the protein. The rapid dilution method is

advantageous, because the pH of the buffer can be adjusted from 9.5 to 4.5 without causing the protein

to precipitate out of solution (this was determined by experimentation). This protein could be loaded directly onto

a cation exchange column and eluted with a pH 4.5 sodium format buffer along with increasing concentrations

of NaCI to purify it, allowing the activity assays to be done without having to cross over the isoelectric point. This

will hopefully help to eliminate the precipitation during the activity assay and yield better results.



Post-anion exchange column activity has not yet been obtained, but this was due to several reasons. The amount

of protein that was loaded on the column was approximately 10.8 mg, much of which was E. coli proteins. Only

a fraction of that was pPM2gPM6, some of which may have failed to correctly refold. In addition to the small

amount of enzyme that could have even been present in the 1.5mL fractions, pPM2gPM6 eluted at around 600

mM NaCI, and the fractions were not desalted before they were tested for activity because the method used to

desalt the fractions lost so much protein. If the experiment were up-scaled, fractions could be pooled and a

different method for desalting could be used that would avoid so much protein loss. Additionally, a strong

anion exchange column was used, and perhaps with a weak anion exchange resin, the protein would still bind,






but would elute with less salt.


ACKNOWLEDGEMENTS



I would like to thank my mentor, Dr. Ben Dunn, Bret Beyer, and the other members of the Dunn lab for

their contributions and guidance.


REFERENCES



1. World malaria situation in 1994. Part I. Population at risk. Wkly. Epidemiol. Rec., 1997. 72(36): pp. 269-274.




2. Bogitsh, B. J., and Cheng, T.C. Human Parasitology, 2nd. Ed. 1998.




3. Rosenthal, Philip J. Proteases of malaria parasites: new targets for chemotherapy. Emerging Infectious

Diseases, 1998. 4(1): pp. 49-57.




4. Gluzman, I.Y., Francis, S. E., et. al. Order and specificity of the Plasmodium falciparum hemoglobin

degradation pathway. J. Clin. Invest., 1994. 93: pp. 1602-1608.




5. Kean, F. H. Cloroquine resistant falciparum malaria from Africa. JAMA, 1979. 241(4): p. 395.




6. Goldberg, D.E., Slater, A.F.G., et. al. Hemoglobin degradation in the human malaria pathogen

Plasmodium falciparum: a catabolic pathway initiated by a specific aspartic protease. J. Exp. Med., 1991. 173:

pp. 961-969.




7. Francis, S.E., Gluzman, I.I. et al. Molecular characterization and inhibition of a Plasmodium falciparum

aspartic hemoglobinase. EMBOJ. 1994. 13: pp. 306-317.




8. Moon, R.P., Tyas L., et al. Expression and characterization of plasmepsin I from Plasmodium falciparum. Eur.

J. Biochem., 1997. 244: pp. 552-560.


9. Silva, A.M., Lee A.Y., et al. Structure and inhibition of plasmepsin II, a hemoglobin-degrading enzyme






from Plasmodium falciparum. Proc. Natl. Acad. Sci. USA, 1996. 93: pp.10034-10039.


10. Davies,D.R. The structure and function of the aspartic proteinases. Annu. Rev. Biophys. Biophys. Chem., 1990.

19: pp. 189-215.




11. Banerjee, Ritu, Liu, Jun, et. al. Four Plasmepsins are active in the Plasmodium falciparum food vacuole, including

a protease with an active site histidine. Proc. Natl. Acad. Sci. USA, 2002. 99(2): pp. 990-995.


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