| Material Information
||An isotope method to determine availability of phosphorus in different phosphatic sources
||4 leaves : ; 28 cm.
||Davis, George K
Arrington, Lewis Robert, 1919-
Outler, Jason Curry, 1922-
University of Florida -- Dept. of Animal Husbandry and Nutrition
||Dept. of Animal Husbandry and Nutrition
||Place of Publication:
||Phosphorus in animal nutrition -- Florida ( lcsh )
Phosphorus -- Isotopes ( lcsh )
||government publication (state, provincial, terriorial, dependent) ( marcgt )
non-fiction ( marcgt )
||Animal husbandry & nutrition mimeograph series - UF Dept. of Animal Husbandry and Nutrition ; no. 57-7
||Statement of Responsibility:
||George K. Davis, L.R. Arrington and Jason C. Outler.
| Record Information
||University of Florida
||All rights reserved by the source institution and holding location.
||oclc - 71303271
Animal Husbandry & Nutrition Mimeroraph June, 1957
Series No. 57-7
AN ISOTOPE METHOD TO DETERMINE AVAILABILITY
OF PHOSPHORUS IN DIFFERENT PHOSPHATIC SOURCES I/.
George K. Davis, L. R. Arrington and Jason C. Outlerl
The need for supplemental phosphorus in many livestock and
poultry rations has resulted in the use of many phosphatic ma-
terials to supply the needed phosphorus. The value of any phos-
phate supplement will depend upon several factors, but the actual
availability to the animal is an important measure of its value.
A number of methods are in use to evaluate utilization or avail-
ability. Most of these involve feeding the test material under
controlled conditions and determining growth and bone composition
of the animal. An isotope method has been designed which affords
a direct measure of absorption. The purpose of this report is to
describe the method and to give some preliminary results.
Three different compounds have been studied with this method.
'Reagent grade dicalcium phosphate (CaHP04) was used as the ref-
erence standard, and two feeding grade phosphates were compared
with the standard.
Samples of the test materials are sent to Oak Ridge either
for two weeks or for one month irradiation in the pile (graphite
reactor). The exposure to neutron (ny) bombardment converts
1Davis, Animal Nutritionist; Arrington, Assistant Animal Nu-
tritionist; and Outler, Assistant in Chemistry, Department of
Animal Husbandry and Nutrition.
phosphorus-31 to phosphorus-32 which is radioactive phosphorus.
Since labeled phosphorus is metabolized in the same way as
natural phosphorus, it may be used to measure quantitatively the
The irradiated material is administered to selected animals
which are confined to digestion stalls so that feces and urine
may be collected separately and quantitatively. The weighed
dose of the radioactive phosphate is administered in a capsule
with a balling gun. Fecal collections are made for six days,
samples are taken, digested and the radioactive phosphorus in the
feces is determined. The amount and activity of that administered
is known so that the amount present in the fecal excretion repre-
sents primarily that which has not been absorbed.
The amount of radioactive phosphorus in the feces subtracted
from that fed to the animal gives an approximate measure of the
phosphorus absorbed since the radioactive phosphorus is a part of
the phosphate compound that is being tested. Total phosphorus de-
terminations are made to provide the full information on comsump-
tion and excretion of phosphorus.
Some phosphorus is absorbed in the upper part of the intestine
and then reexcreted back into the digestive tract. This appears
in the feces as unabsorbed phosphorus. For a measure of total
absorbtion, it is necessary to measure the phosphorus which is
absorbed and reexcreted. This reexcreted phosphorus increases as
animals get older and is especially important for the evaluation
of phosphorus used for livestock and poultry after the very early
A measurement of the phosphorus absorbed and reexcreted is
possible by use of the radioactive phosphorus. If the rate of
reexcretion does not change rapidly, then the same animal used
at two different periods can provide an estimate of the phospho-
rus excretion into the intestinal tract.
This measurement has been accomplished by injecting radio-
active phosphorus in phosphates into the blood of the animal.
The phosphorus is rapidly mixed with the blood and radioactive
phosphorus appearing in the feces is then a measure of the phos-
phorus excreted from the blood. Since the radioactive phosphorus
acts in the same manner as stable phosphorus and is a measured
proportion of blood phosphorus, this gives us a measure of total
phosphorus excreted that had been absorbed into the blood.
If the phosphorus, reexcreted from the blood, is similar for
all sources of dietary phosphorus, then fecal phosphorus compared
to consumed phosphorus may be used as an approximate measure of
absorbed phosphorus but the values will always be less than was
actually absorbed. Since this may not be true, experiments to
check this are being continued.
One of the questions which has been raised regarding this
method is whether or not irradiation in the pile changes the form
of the phosphate. If the amount of the ortho form is increased
or decreased during irradiation, the results would have to be cor-
rected to account for the change. Tests of the materials after
irradiation have thus far shown no change, but these are being
Comparative results using the three phosphates are recorded
in Table I. These data represent absorption which is calculated
by subtracting total fecal excretion from the dose administered.
If the values were corrected for that portion of phosphorus that
is absorbed and reexcreted, then the values would be somewhat
% Absorbed by
Calves at 144 hours
% Absorbed by
Rats at 72 hours
These results should not be interpreted as conclusive but
as preliminary. Tests are being continued as facilities and ani-
mals become available.