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FOREWORD We extend to you a cordial welcome to this Twenty-third Citrus Processors' Meeting, sponsored jointly by the Florida Department of Citrus Scientific Research Department and the IFAS Agricultural Research and Education Center at Lake Alfred. Our objectives in these meetings are to keep citrus processors up- to-date on our research results, and to listen carefully to the processors' comments and ideas about what our program should be. It is difficult to keep up to date and relevant in a small research group. Opportunities to modernize or change direction are greatest when new personnel enter the research group. We hope to keep closely in touch with the processing industry, so that our decisions about change in direction will be most likely of success Herman J. Reitz Director Agricultural Research and Education Center, Lake Alfred **** ********************* **** The Department of Citrus staff joins the Institute of Food and Agricultural Sciences in welcoming you to the Annual Processors' Meeting. We have a number of interesting reports for you this year including the results of our work on grapefruit bitterness, the second year's data from our Juice Definition Program and the status of the Automatic Brixometer. We of the Department of Citrus again express our deep appreciation for the continuing help and cooperation extended to us by IFAS Agricultural Research and Education Center during the past season. John A. Attaway Scientific Research Direct Florida Department of Citrus PROGRAM University of Florida Agricultural Research & Education Center P. 0. Box 1088 Lake Alfred, Florida 33850 9:00 A.M. Registration 9:30 A.M. Welcome Herman J. Reitz Director, Agricultural Research & Education Center Lake Alfred, Florida. Chairman: Art Mathias, Chairman, Florida Citrus Research Council. 9:45 A.M. FLUOROMETRIC DETERMINATION OF LIMONIN IN CITRUS JUICE PRODUCTS James F. Fisher, Research Chemist, Florida Department of Citrus, Lake Alfred. A new procedure for the assay of microgram quantities of limonin in grapefruit juice will be presented. This determination of limonin is more objective than earlier methods. Limonin was separated from grapefruit juice and quantitated fluro- metrically as follows: 1. Grapefruit juice was extracted with chloroform. 2. The chloroform was removed and the residue chromatographed on a short aluminum oxide column employing a chloroform/ benzene solvent system. The column retained the compounds which were more polar than limonin. 3. The eluate, containing the limonin and less polar compounds, was treated with aqueous sodium hydroxide. This converted the limonin into the di-sodium salt of limonoic acid which was extracted into the aqueous phase leaving the unwanted less polar material remaining in the chloroform layer. 4. The di-sodium salt of limonoic acid was reconverted to limonin by acidification of the aqueous phase followed by chloroform extraction. This afforded limonin in the chloroform layer. 5. The isolated limonin was measured fluorometrically in concen- trated sulfuric acid. 6. The micrograms of limonin were read from a standard curve. The mean of five determinations on a grapefruit sample was 4.06 ppm with a range of from 3.84 to 4.36 ppm. This difference of 0.52 ppm is good precision for this type of procedure. 10:00 A.M. VITAMIN E CONTENT OF COMMERCIAL CITRUS JUICES - R. J. Braddock, Assistant Food Scientist, and J. W. Kesterson, Chemist, University of Florida Agricultural Research and Education Center, Lake Alfred. Tocopherol (vitamin E) analyses were performed on a variety of fresh and processed citrus fruits. A sensitive, gas chromatographic tech- nique was employed to quantitatively determine the tocopherol content of these products. The technique involved the identification and esti- mation of the volatile trimethylsilyl ethers of individual tocopherols present in a sample. The tocopherol content measured as mg total tocopherol/100 ml juice, was as follows for the various samples: 1) reconstituted commercial frozen concentrated orange juice (13 samples, 0.01-0.14), 2) glass-pac single strength orange juice (4 samples, 0.1- 0.2), 3) glass-pac single strength grapefruit juice (4 samples, 0.1- 0.2), 4) canned single strength orange juice (2 samples, 0.2), 5) canned single strength grapefruit juice (2 samples, 0.2), 6) fresh-squeezed, unheated 'Valencia' juice (4 samples, 0.3-0.8) and 7) fresh-squeezed, unheated 'Duncan' grapefruit juice (4 samples, 0.15-0.3). Peel oil and seed oil from fresh fruit were much higher in tocopherols than the juices, generally having values in the range 5-30 mg tocopherols/100 gm sample. For purposes of comparison, the tocopherol content of cotton- seed oil is about 50-60 mg/100 gm and wheat germ oil is in the range 150-200 mg/100 gm. These results indicated that citrus juices were not good dietary sources of vitamin E, primarily because of low concentration. Compari- sons between fresh and processed juices also showed that processing significantly decreased the amounts of tocopherols present. 10:15 A.M. THE USE OF THE CENTRIFUGE FOR FINISHING CITRUS JUICES - M. D. Maraulia, Chemist II, Florida Department of Citrus, Lake Alfred. During the 1970-71 citrus season, nine fruit samples of 'Valencia' and 'Pineapple' oranges were extracted with commercial equipment using a harsh, destructive squeeze, and, both hard and soft "commercial" squeezes. Following extraction, the pulpy juices were finished using both an FMC Model 35 screw-finisher and a Westphalia, Model SA7, pilot plant size centrifuge. The two finishing methods were compared on the basis of single-strength finished juice quality and on that of the high Brix concentrates in regard to flavor, cloud, bottom pulp, viscosity and color, together with finished juice yields. The over-all quality of the centrifuge-finished juices was dependent for the most part on the degree of finishing as compared with screw-finishing using standard screen and settings. When juice yields by both finishing methods were matched to within approximately 1% yield, the overall quality of the centrifuge-finished juices was slightly higher. High Brix concentrates prepared from these juices using both a hard and soft squeeze were con- siderably less viscous than those prepared from the screw-finished juices. Some quality factors for pulp-free, high ratio, Valencia con- centrate, prepared by centrifuge finishing, are also discussed. 10:30 A.M. REFRACTIVE INDEX SOLUTIONS FOR STANDARDIZATION OF ELECTRONIC REFRACTOMETERS USED IN STATE TEST JUICE ROOMS R. W. Wolford, Research Chemist, and J. G. Blair, Research Engineer, Florida Department of Citrus, Lake Alfred. Investigations leading to the development of a stable chemical solution for use in the routine standardization of Electron Machine Corporations (EMC) SSR-70 Refractometers were conducted in cooperation with personnel of the Florida Department of Agriculture, Division of Fruit and Vegetable Inspection during the latter part of the 1971-72 processing season. The commercial EMC units which have been in operation in State Test Juice Rooms in 12 processing plants this season have had as the only standardizing media fresh juice, or juice plus con centrate, to provide an approximate 100 Brix span from 70 to 170 Brix. The many inherent problems encountered using juices of variable con- sistency for absolute calibration of the digital readout system within the tolerance limits of + 0.05 Brix made it evident that some appro- priate external standard having physical characteristics similar to orange and/or grapefruit juices was required. Intensive research concurrent with optical and electronic adjust- ments to the Department of Citrus EMC Refractometer in this laboratory resulted in the development of a solution, or series of solutions, of specific refractive indices containing cloud and color (optional) and having a specific gravity closely comparable to that of citrus juices. Laboratory comparisons between the Department of Citrus EMC Refracto- meter and an updated commercial EMC system, both calibrated using the new refractive index solutions, showed excellent correlation with Brix hydrometer values (within 0.050 Brix) on fresh and processed single strength juices. The refractive indices (relative 0 Brix) standards were found to be applicable for instrumental calibration of several commercial systems at the EMC plant. The useage of the standards for electronic adjustment, linearity checks, and simulated juice analyses by the manufacturer and test room operating personnel was successful. Statistical data developed during laboratory and commercial testing in the 1971-72 season will be presented. OJ BREAK 11:20 A.M. PHYSICAL AND CHEMICAL CHARACTERISTICS OF COMMERCIAL FCOJ PACKED DURING THE 1971-72 SEASON R. W. Barron, Chemist II, Florida Department of Citrus, Lake Alfred. A total of 203 samples of commercial FCOJ were examined for flavor and color during the 1971-72 citrus season. In addition, physical and chemical analyses relating to juice quality were conducted. Samples were collected semi-monthly during the season from 10 to 20 plants out of 22 plants in Florida. Reconstituted juices were graded for flavor by an Agricultural Research and Education Center taste panel. The per- centages of Flavor grades for samples in the "like very much", "like moderately", "like slightly", "neither like nor dislike", or "dislike slightly" category were respectively 8, 62, 26, 3, and 1 for the 1971- 72 season; 4, 60, 30, 5, and 1 for the 1970-71 season; and 1, 24, 55, 19, and 1 for the 1969-70 season. Color differences in the reconstituted juices were measured with a Hunterlab Citrus Colorimeter. The average Citrus Red and Citrus Yellow values were higher this season than last season and about the same as for 1969-70. 11:35 A.M. GRAPEFRUIT JUICE BITTERNESS STUDIES S. V. "Jerry" Ting, Research Biochemist, John A. Attaway, Scientific Research Director, James G. Blair, Research Engineer, Robert D. Carter, Chemist II, Paul Fellers, Food Technologist, and James F. Fisher, Research Chemist, Florida Department of Citrus, Lake Alfred. A new study of the relationship of the major bitter principles of grapefruit juice and juice products to quality has been initiated. The purpose of this program is to determine the desirable levels of bitter- ness in grapefruit products and to develop objective methods for evalu- ating grapefruit flavor through analysis for bitter constituents. The 2 known major chemical constituents which contribute to bitter taste in grapefruit juice are limonin and naringin. The latter is the principal flavanone glycoside of grapefruit. It is generally believed that limonin is much more bitter than naringin and makes a major con- tribution to poor fruit quality in the early weeks of the season. Efforts have been made to develop a bitterness index by adding the ppm limonin, multiplied by an appropriate factor, to that of naringin to give a single value as suggested by Mr. Robert Kilburn of Citrus World, Inc. This approach was not completely successful due to dis- agreement among investigators concerning the magnitude of the factor, to a lack of reliable methods for accurate analysis for limonin, and to the question of correlation between the Davis test for flavanone and the actual naringin content. The relationship of limonin values to flavor reported in this study can only be considered approximate, however, juices with a high limonin content and a high bitterness index frequently were given lower flavor scores by the taste panel at the Center. An important part of this study involved 2 experiments in a plant with a commercial pulp-wash unit. One experiment was conducted in mid-December and the other in late April. In the December experiment, reconstituted pulp-wash concentrate contained up to 20 ppm limonin while that prepared in April contained 0-5 ppm. Some early-season commercial grapefruit pulp-wash contained as much as 29 ppm limonin. Centrifuging these samples lowered the limonin level, however, in the April experiment, the effect of centrifuging on limonin content of concentrated pulp-wash was inconclusive. Unprocessed early season juice showed about 5-7 ppm limonin while none could be detected in un- processed late season juice. A wide variety of commercial grapefruit juice samples, concentrated, chilled, and canned, from Florida markets, as well as from 4 major National markets, were analyzed for bitter principles. The results were compared with flavor panel scores expressed via a 9-point Hedonic Scale. Sugar-add samples were excluded. No direct correlation could be demonstrated, but it was found that reconstituted grapefruit juice and chilled grapefruit juice received substantially higher scores than canned juice. An extractor study conducted in late December using light, medium, and hard squeeze settings with both 'Duncan' and 'Marsh' varieties, demonstrated that limonin values were highest with the hard squeeze regardless of variety. In this study, it was also found that the 'Duncan' juice contained less limonin than the 'Marsh' juice. Finisher tests with these juices gave inconclusive results regarding the re- duction of bitterness. It was generally observed that when grapefruit juice had a high Brix and Brix/acid ratio, the taste panel was less likely to discrimi- nate against it on the basis of bitterness than when it had a low Brix and Brix/acid ratio. To provide insight regarding the bitterness level preferred by con- sumers, grapefruit juice with 4 different levels of bitterness were provided to the U.S.D.A. consumer-type taste panel in Washington. It was found that the U.S.D.A. tasters were much more tolerant toward bitterness than the Center's panel. As a consequence, an extensive central location taste test is planned for Winter 1972-73 to get more information regarding the degree of bitterness the grapefruit juice consumer will accept. 11:50 A.M. PROGRESS OF PROCESSED CITRUS JUICES IN WESTERN EUROPE - Howard J. Connolly, International Marketing Manager, Florida Department of Citrus, Lakeland. The greatest stimulus to the consumption of processed citrus juices occurred during and immediately after World War II. It was the result of the massive shipments of juices made to Europe during 1943-44, and then subsequently, the availability of canned citrus juices, that were available through the period of postwar reconstruction. In the late 1940's the citrus industry in Florida found themselves with overproduction in canned citrus juices and recognizing the potential that the European market offered took positive action in trying to develop sales for canned juices. This effort was supple- mented by the efforts of the then Florida Citrus Commission, which established an office in Europe and generated a program of generic advertising and promotion. This kind of activity continued well into the middle 1960's, and the one problem that had not been completely satisfied was the continuity of supply that was and is necessary to sustain any marketing activity. In the mid-1960's the market development program, a joint effort of the Foreign Agricultural Service, the Florida Department of Citrus and the foreign importer was initiated and has since been identified as the Three Party Program. While at the outset it primarily supported single strength juice in glass and some retail-size frozen concentrated orange juice, as competitive activity increased, there was the recog- nition of a market change. The Florida effort was faced with lower prices from other citrus-producing areas of the world, which brought about the recognition of the need for introducing bulk frozen concen- trated citrus juices for reconstitution for the European marketing area. In the last three years, a major transition has been made in many of the Western European countries with regard to citrus juices. They are now assuming a regular place in the eating habit of the European consumer, and because of the readily available channels of distri- bution offered by many large dairies which are reconstituting bulk frozen juice into single-strength in paper cartons, we now have the two ingredients necessary for increased consumer activity. These are availability, because of good distribution, and competitive pricing due to the program of reconstitution from bulk. 12:15 P.M. L------------------------------------ 12:15 P.M. LUNCH ------------------------------------- Chairman: 1:30 P.M. Marvin Walker, Vice Chairman, Florida Citrus Commission. THE JUICE DEFINITION PROGRAM, SECOND ANNUAL REPORT - John A. Attaway, Scientific Research Director, Rodger W. Barron, Chemist II, James G. Blair, Research Engineer III, Bdla S. Buslig, Research Biochemist, Robert D. Carter, Chemist II, Marshall H. Dougherty, Research Engineer II, Paul Fellers, Food Technologist, James F. Fisher, Research Chemist, Elmer C. Hill, Research Bacteriologist, Richard L. Huggart, Chemist III, Donald R. Petrus, Chemist III, S. V. "Jerry" Ting, Research Biochemist, Florida Department of Citrus and Alvfn H. Rouse, Chemist, Agricultural Research and Education Center, Lake Alfred. In a study to determine the typical chemical and physical juice characteristics of orange juice in the 1970-71 season, 33 separate, analyses were made on two series of juices prepared periodically in the pilot plant. One series had been produced using extremely harsh squeezing which would generally be considered unacceptable in commercial practice, while the other was prepared in a manner approach- ing that used in commercial production. These two juices were found to be widely divergent in many of their characteristics. During the past season 31 analyses were used to study another two sets of juices prepared with two extractor settings less different than those used in the previous year. Both of these settings were within the bounds of commercial production practices. In addition, juices were prepared using the "State Test-House" extractor. Compari- son with the latter series of juices will be reported later by James G. Blair. The following table shows a comparison of the conditions of the juice extractor setting for the past two seasons using 1/8" beam settings. 1970-71 Season 1971-72 Season Squeeze Squeeze Harsh Soft Hard Light Cups 3" 3" 3" 4" 3" 4" Strainers 090" 040" 040" 040" 040" 040" Tubes 1" WR 1" SR 1" WR i 1/4"WR 1" SR 1 1/4"SR ID and/or Restrictor 7/16"LR 1/2"BM 7/16LR 1/2"LR 5/8"BM 9/16"LR Upper Cutter S S S S L L Note: WR Window Tube SR Split Ring (No window) LR Long Restrictors BM Bell Mouth L Long S Short In a separate series of tests, the 1970-71 soft squeeze settings were repeated periodically to provide a comparison of the fruit from the two years. The long upper cutter and the larger diameter strainer tube of the light squeeze were found to give comparatively lower yields. A uniform representation of the three varieties, 'Hamlin', 'Pine- apple' and 'Valencia' was used this past year, while only one compara- tive run had been made with the 'Hamlin' variety in 1970-71 because of problems encountered in starting up the program. The State test juices were finished periodically as will be discussed in detail in the next presentation. This work indicated the need for inclusion of finisher setting variables in the experimental design for the 1972-73 Juice Definition Program. A table of maximum, minimum and average values for each parameter in the 1971-72 program is provided and a comparison made with the correspond ing values in the 1970-71 program. A table of correlation coefficients for each variety will be presented for the first time this year. Using the correlation coefficients, a Multiple Regression Flavor Prediction Equation (MRFPE) was developed by means of a computer program. The agreement of actual flavor scores and the flavor scores predicted by the equation compares favorably with a similar equation developed from the 1970-71 data. In an effort to make a practical, commercial application of the MRFPE 30 samples of commercial FCOJ were analyzed and correlation coefficients developed. This will be presented in the paper by Fellers and Buslig. A number of new types of analyses, are being evaluated using the juices stored throughout the season. The most promising of these analyses will be added to the program for next year. 2:10 P.M. PREDICTION OF ORANGE JUICE FLAVOR USING A MULTIPLE REGRESSION EQUATION Paul J. Fellers, Food Technologist, and Bdla S. Buslig, Research Biochemist, Florida Depart- ment of Citrus, Lake Alfred. The reconstituted juices of 30 commercial frozen concentrated orange juice samples collected during the 1970-71 citrus season were analyzed for 30 different characteristics. The analyses included flavor, sink- ing pulp, Brix, acid, Brix/acid ratio, oil, serum viscosity, total aldehydes, oxygenated terpenes, Chemical Oxygen Demand (C.O.D.), total glycosides, limonin, pH, cloud, color values from the Hunterlab Citrus Colorimeter as follows: Citrus Red (CR), Citrus Yellow (CY), and Equivalent Color Score (ECS); water insoluble solids, alcohol insolu- ble solids, sucrose, total sugars, protein, water soluble pectins, sodium hydroxide soluble pectins, total pectins, ash, potassium, sodium, calcium and magnesium. Flavor was determined in each case by 10 experienced taste-panelists evaluating samples on a 9-point hedonic scale. All other determinations were performed by established analytical procedures. None of the samples were found to contain limonin, subsequently this variable was omitted from the statistical analysis. The General Electric (G.E.) time-sharing computer was utilized in analyzing the data. Correlations of individual variables with flavor yielded a maximal correlation coefficient of -0.529 with total glyco- sides. Such a correlation explained only about 28% of the flavor variation, therefore further analysis considering multiple variables was employed. The G.E. Statistical Analysis System used a stepwise linear multiple regression in which a maximum of 25 independent variables were permitted at one time. The system automatically selected a combination of 6 variables giving the highest degree of significance contained in the data. These 6 variables yielded the following regression equation: (FL) = 1.076(AC) + .956(TS) .676(NA) + .915(CA) + .02(TP) - .045(GLY) 4.626 FL = flavor AC = % acidity CA = calcium TS = total sugars TP = total pectin NA = sodium GLY = total glycosides r(correlation coefficient) = .904 -10- Using the above equation, flavor scores were calculated and the table below shows a comparison between actual and predicted scores. (The equation predicted approximately 81.5% of the variation in flavor.) Flavor Scores of Samples Observed Predicted Observed Predicted 6.7 6.6 6.1 6.4 6.4 6.4 7.0 6.8 6.1 5.9 7.0 6.8 6.2 6.4 6.4 6.6 7.1 7.0 7.1 7.2 6.3 6.1 7.6 7.5 7.0 6.9 5.5 5.4 6.7 6.8 5.8 6.2 6.6 6.6 6.3 6.6 6.6 6.8 7.0 6.7 5.1 5.4 6.1 6.6 7.5 7.2 6.9 6.7 6.8 6.6 6.5 6.3 6.9 7.0 6.5 6.9 6.3 5.8 5.4 5.4 Further statistical analyses, excluding the previous six variables, showed no other statistically significant components in either linear or 2 different curvilinear regression systems. OJ B R E A K 2:45 P.M. GRAPEFRUIT POUNDS-SOLIDS James G. Blair, Research Engineer III, Florida Department of Citrus, Lake Alfred. The Inspection Service has been voluntarily fulfilling an increasing number of requests for pounds of solids measurements on grapefruit. This very likely will result in a regulation making the measurement of grapefruit Pounds-Solids a mandatory procedure for all processing plants requesting State Certification. Several years ago evaluations of extractors were made for the purpose of selecting a machine for the Pounds-Solids measurement of oranges. Limited tests were run on grapefruit but no in-depth study was carried out because at that time there was little interest in Pounds-Solids measurement for this fruit. -11- Two years ago the industry requested that a program be started with the objective of improving the current methods of measuring grapefruit Pounds-Solids. So far the efforts on this program have been confined to the modification of the present official extractor to obtain more equitable yields between the Marsh and Duncan varieties. The increase in squeezing pressure which worked well for oranges, was not sufficient for grapefruit especially when only the 5-inch cup was used for all fruit. Therefore, it was necessary to broaden the testing procedures to include the use of both the 4- and 5-inch cups as well as different strainer tubes and settings. The results of these new developments are encouraging and it appears that progress is now being made toward accomplishing the objectives. 3:00 P.M. ANALYTICAL COMPARISON OF COMMERCIAL ORANGE CONCENTRATE AND ORANGE PULP WASH CONCENTRATE PRODUCED DURING THE 1970-71 AND 1971-72 SEASONS Robert D. Carter, Chemist II, A. H. Rouse, Chemist, S. V. Ting, Research Biochemist, and Rodger W. Barron, Chemist II, Florida Department of Citrus and Agricultural Research and Education Center, Lake Alfred. Orange pulp wash concentrate and samples of non-pulp wash orange concentrate evaporator pumpout were compared using 33 analytical pro- cedures of which only 5 showed significant differences. Twenty-three midseason samples and twenty-nine'Valencia' samples were collected from seven commercial concentrate plants during the 1970-71 and 1971- 72 seasons. Striking differences between pulp wash and non-pulp wash concen- trate samples were found in the values obtained for serum viscosity, water-insoluble solids, protein, water-soluble pectin, and oxalate- soluble pectin (1970-71 only). Regression equations were developed using results for the above constituents. The data points are dis- played graphically below and the equations will be discussed in the presentation. CONSTITUENT ANALYSES ILLUSTRATING DIFFERENCES BETWEEN ORANGE PULPWASH CONC. AND NON-PULPWASH PUMPOUT CONC. 52 SAMPLES-7 PLANTS-1970-71 and 1971-72 SEASONS SVP W 1 _VPO M=Midseason VPW VPO V = Valencia M P W M PO po=Pumpout Conc. PROTEIN ( g/100ml X 100) PW=Pulpwash Conc. VPO overlap IV VPw SMPW MPO SERUM VISCOSITY cps 0 5 10 15 20 25 VPO MPO MPW OXALATE-SOLUBLE PECTIN (mg/100g) VPO VPW MPO I MPW overlap/ WATER-SOLUBLE PECTIN (mg/ IOOg) VPW VPO MPW IMPO WATER-INSOLUBLE SOLIDS (mg/IOOg) I I I I I 0 50 100 50-200 25 .150 200 250 0 50 100 -13- 3:15 P.M. FLAVOR COMPARISON James G. Blair, Research Engineer III, Florida Department of Citrus, Lake Alfred. The Juice Definition Program (JDP) has been in operation at the Center for two complete seasons. Juices for this program were ex- tracted at different pressure settings. It was found that a change in these settings usually affected both yield and flavor. To pro- vide a basis for comparing JDP yields with those attained in commercial operations, an official State Test Room Extractor was used as a control. Juices from the program had flavor scores ranging from "dislike extremely" for samples prepared by a harsh squeeze to "like moderately" for samples prepared using a soft squeeze. Surprisingly, the taste panel expressed a preference for juices from the State Test Room Extractor over any of the juices tested. Some juices had a relatively high pulp content which normally detracts from flavor. However, some consumers prefer juice with pulp, particularly the floating type. The pulp from the State Test Extractor is of this type. The characteristics of the pulp have a definite bearing on flavor and consumer acceptance. Some primary differences between the commercial extractors used and the State Test House Extractor include the hole size in the strainer tube, the internal diameter of the orifice tube, the machine speed and the length of stroke. Finishers were adjusted to yield juices with the same Quick Fiber value regard- less of extractor setting, therefore it was assumed that the finisher did not represent a variable. These Abstracts are for limited distribution only. Information herein is not to be used for publication without permission. Acknowledgment for helpful assistance is made to Fred Schopke, Ben Wood, Irene Pruner, Betty Murphy, Mary Smith, Alice Barber, Florence Wolff, Margaret Swift, Sharon Lovejoy, Roger Waters, Joe Collins, Fred Givens, Roy Albright, Harold Walker, Vernon Rhoades, and to all other personnel of either the University of Florida Agricultural Research and Education Center or the Florida Department of Citrus who helped in many and various ways. -14- NOTES |