Material Information

The Relationship between Periplasmic Nitrate Reductase and the Length of Diauxic Lag in Denitrifying Bacteria Switching from Oxic to Anoxic Conditions
Fischer, Kyle
Publication Date:


Subjects / Keywords:
Anaerobic conditions ( jstor )
Bacteria ( jstor )
Denitrifying bacteria ( jstor )
Nitrates ( jstor )
Paracoccus pantotrophus ( jstor )
Wastewater treatment ( jstor )
Undergraduate Honors Thesis


The efficiency of biological denitrification processes in wastewater treatment is adversely affected by the length of diauxic lag when denitrifying bacteria are switched from oxic to anoxic conditions. Periplasmic nitrate reductase (Nap) is hypothesized to shorten diauxic lag. A Nap-containing bacterium and a Nap-deficient mutant of the same strain were grown and the lengths of their diauxic lags were compared to test this hypothesis. Nap containing (wild type) Paracoccus pantotrophus displayed a significantly shorter diauxic lag than the Nap-deficient mutant bacteria. This result supports the hypothesis that the presence of Nap in bacteria decreases the length of diauxic lag. Enrichment of Nap containing bacteria in wastewater treatment facilities could increase treatment rates by reducing diauxic lags. ( en )
General Note:
Awarded Bachelor of Science in Chemical Engineering; Graduated May 4, 2010 summa cum laude. Major: Chemical Engineering
General Note:
Advisor: Dr. Ben Koopman
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College/School: College of Engineering
General Note:
Legacy honors title: Only abstract available from former Honors Program sponsored database.

Record Information

Source Institution:
University of Florida
Holding Location:
University of Florida
Rights Management:
Copyright Kyle Fischer. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.