Material Information

DNA Origami: The Modified Icosahedron
Gelfand, Brittany
Publication Date:


Subjects / Keywords:
Biochemistry ( jstor )
Cooling ( jstor )
Engineering ( jstor )
Food staples ( jstor )
Heating ( jstor )
Icosahedrons ( jstor )
Oligonucleotides ( jstor )
Origami ( jstor )
Software ( jstor )
Software applications ( jstor )
Biochemical engineering
Undergraduate Honors Thesis


DNA origami is a means of creating multi-dimensional shapes on the nanoscale level. This process involves DNA folding to create larger DNA designs. Single strands of DNA consisting of thousands of base pairs are forced to take specific shapes using smaller strands of DNA, called oligonucleotide staple strands. The shapes are created by denaturing the DNA strands through heating and then cooling once they are in the proper environment. At this point, the strands of DNA will assume the prescribed orientation. This thesis paper specifically discusses the development of a new three-dimensional shape, the modified icosahedron, based on the structure of the previously created icosahedron. Three-dimensional DNA origami structures can be created and viewed using a relatively new software program called caDNAno. The program allows for DNA template strands to be selected and combined in the desired configuration. Once the backbone of the structure is completed, the program adds staple strands, which hold the template together, and also provides sequences for synthetic strands. The sequences that are given by the software are used to synthesize the oligonucleotides in the laboratory. The three-dimensional DNA complexes that are created can be used for specific applications in biochemical engineering, such as drug delivery. ( en )
General Note:
Awarded Bachelor of Science in Chemical Engineering; Graduated May 4, 2010 summa cum laude. Major: Chemical Engineering
General Note:
College/School: College of Engineering
General Note:
Legacy honors title: Only abstract available from former Honors Program sponsored database.
General Note:
UF Honors Program sponosored database

Record Information

Source Institution:
University of Florida
Rights Management:
Copyright Brittany Gelfand. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions require