Citation

Material Information

Title:
The effect of galacto-oligosaccharide supplementation on intestinal microbiota
Creator:
Bost, Madeline
Publication Date:
Language:
English

Subjects

Subjects / Keywords:
Anticipation ( jstor )
Bacteria ( jstor )
College students ( jstor )
Diseases ( jstor )
Electrophoresis ( jstor )
Gastrointestinal tract ( jstor )
Gels ( jstor )
Prebiotics ( jstor )
rRNA genes ( jstor )
Undergraduate students ( jstor )
College students
Diet
Prebiotics
University of Florida
Genre:
Undergraduate Honors Thesis

Notes

Abstract:
Supplementation with prebiotics, substrates intended to facilitate the growth of beneficial bacteria in the gastrointestinal tract, is anticipated to affect health and reduce disease complications. The purpose of this project was to study an intervention in the diets of University of Florida undergraduate students by providing galacto-oligosaccharide (GOS) fiber packets to consume daily. Of the 427 students participating, a subgroup of 66 individuals provided a total of 262 stool samples. DNA was extracted from fecal samples, and the 16S rRNA gene was amplified non-selectively from the bacterial community and analyzed using denaturing gradient gel electrophoresis (DGGE). Baseline and treatment samples were compared for each subject. According to DGGE analysis using both visual comparison and statistical tests GOS intake significantly affected the diversity of intestinal microbiota. Ongoing DNA sequencing will provide additional data on which species were affected. ( en )
General Note:
Awarded Bachelor of Science; Graduated December 21, 2010 magna cum laude. Major: Food Science and Human Nutrition, Emphasis/Concentration: Nutritional Sciences
General Note:
Advisor: Volker Mai
General Note:
College of Agricultural and Life Sciences
General Note:
Legacy honors title: Only abstract available from former Honors Program sponsored database.

Record Information

Source Institution:
University of Florida
Rights Management:
Copyright Madeline Bost. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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