Evaluation of Novel Potassium Channel-Directed Compounds as New Mosquitocides for Control of Aedes aegypti and Anopheles...

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Material Information

Title:
Evaluation of Novel Potassium Channel-Directed Compounds as New Mosquitocides for Control of Aedes aegypti and Anopheles gambiae
Physical Description:
1 online resource (78 p.)
Language:
english
Creator:
Larson, Nicholas R
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Master's ( M.S.)
Degree Grantor:
University of Florida
Degree Disciplines:
Entomology and Nematology
Committee Chair:
BLOOMQUIST,JEFFREY R
Committee Co-Chair:
BERNIER,ULRICH R
Committee Members:
KAUFMAN,PHILLIP EDWARD

Subjects

Subjects / Keywords:
aedes -- aegypti -- anopheles -- gambiae -- insecticides -- potassiumchannel
Entomology and Nematology -- Dissertations, Academic -- UF
Genre:
Entomology and Nematology thesis, M.S.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Vector control with insecticides is in a constant struggle to stay ahead of resistance development, so new insecticides with novel modes of action are needed. A novel class of target site for insecticide development would be potassium channels of nerve and muscle membranes. In this study, known and experimental potassium channel blockers were tested for toxicity against Anopheles gambiae and Aedes aegypti mosquitoes. 4-Aminopyridine was found to have the closest toxicity (10-fold less) to the industrial insecticide propoxur in a new headless larvae paralysis assay. Catechol PRC 725 was the most toxic experimental compound in all assays, but was at least 100-fold less toxic than propoxur in Ae. aegypti and 39-fold in An. gambiae. Potassium channel blockers increased tension in larval muscle sheets, along with increased duration of evoked contractions. For example, tetraethylammonium chloride (30 mM) increased baseline muscle tension and evoked contraction duration upwards of 200% and 500%, respectively. A novel symptom of melanization occurred after exposure to catechol compounds in the larval mortality assays, which may indicate their metabolism by phenoloxidase, followed by chemical polymerization. Compared to An. gambiae adults, toxicity of the compounds decreased significantly when screened against Ae. aegypti. Injection and synergist studies indicated that the decreased sensitivity was not primarily due to the cuticle acting as a barrier, nor by metabolic degradation of the compounds. While the toxicity of the experimental catechols was modest, the chemical structures of these compounds may prove to be useful leads for the development of more powerful compounds.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Nicholas R Larson.
Thesis:
Thesis (M.S.)--University of Florida, 2014.
Local:
Adviser: BLOOMQUIST,JEFFREY R.
Local:
Co-adviser: BERNIER,ULRICH R.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2015-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0046744:00001