Identification and Analysis of Potential Sex-Determination Genes in Metaseiulus occidentalis (Acari

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Material Information

Title:
Identification and Analysis of Potential Sex-Determination Genes in Metaseiulus occidentalis (Acari Phytoseiidae)
Physical Description:
1 online resource (112 p.)
Language:
english
Creator:
Pomerantz, Aaron F
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Master's ( M.S.)
Degree Grantor:
University of Florida
Degree Disciplines:
Entomology and Nematology
Committee Chair:
HOY,MARJORIE ANN
Committee Co-Chair:
KAWAHARA,AKITO

Subjects

Subjects / Keywords:
chelicerata -- evolution -- phytoseiidae -- sex-determination
Entomology and Nematology -- Dissertations, Academic -- UF
Genre:
Entomology and Nematology thesis, M.S.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Little is known about the process of sex-determination at the molecular level in Metaseiulus occidentalis, a predator of phytophagous pest mites. Detailed knowledge of the sex-determination pathway could allow for genetic manipulation of M. occidentalis to produce more female offspring, which could improve its effectiveness as a biological control agent. By screening the genome and transcriptome, potential sex-determination genes were identified: four doublesex-mab-3-related transcription factor (dmrt) genes, one transformer-2 gene, one intersex gene, and two fruitless-like genes. Comparative analyses reveal conservation within functional domains and confirmed that the architecture for certain sex-determination genes is conserved in chelicerate arthropods. Eleven genes were evaluated by reverse-transcriptase PCR to determine if this mite exhibits sex-specific gene expression. Single transcripts for doublesex1, doublesex2, transformer-2, intersex, and fruitless1-like were detected from cDNA constructed from male embryos, male deutonymphs and adults, and female deutonymphs and adults, suggesting that pre-mRNA of these genes does not undergo alternative splicing in a sex-specific manner. Four vg-like genes were detected from cDNA from adult females only, while two other vg-like genes were detected from cDNA from both adult males and females. An RNA interference study was carried out to evaluate the biological role of transformer-2 (tra-2) through oral delivery of double-stranded RNA (dsRNA) to adult males and females. Females that ingested tra-2 dsRNA produced significantly fewer eggs compared to control females. The sex ratio of progeny was not significantly altered, suggesting that tra-2 may not be implicated as a major regulatory gene in the sex-determination pathway.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Aaron F Pomerantz.
Thesis:
Thesis (M.S.)--University of Florida, 2014.
Local:
Adviser: HOY,MARJORIE ANN.
Local:
Co-adviser: KAWAHARA,AKITO.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2015-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0046729:00001