Physical and Biochemical Alterations of Heat Exposed Bone in Relation to Ultraviolet Fluorescence

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Material Information

Title:
Physical and Biochemical Alterations of Heat Exposed Bone in Relation to Ultraviolet Fluorescence
Physical Description:
1 online resource (8 p.)
Language:
english
Creator:
Van Deest, Traci L
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Anthropology
Committee Chair:
WARREN,MICHAEL WADE
Committee Co-Chair:
KRIGBAUM,JOHN
Committee Members:
DAEGLING,DAVID
BURT,MARTHA
KAMENOV,GEORGE DIMITROV

Subjects

Subjects / Keywords:
anthropology -- bone -- burned -- fluorescence
Anthropology -- Dissertations, Academic -- UF
Genre:
Anthropology thesis, Ph.D.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Alteration to bone due to heat exposure is important to correctly interpret burned and cremated remains from modern and archaeological contexts. This research examines changes to heated bone tissue to investigate underlying mechanisms of fluorescence under ultraviolet light. Five porcine femora were cleaned of all soft tissue and sectioned into 13 segments. To examine temperature variation, heat treatment included one unheated control sample and a segment heated at one of the following temperatures: 100 degrees C, 300 degrees C, 500 degrees C, 700 degrees C, and 900 degrees C in for 60 minutes. To test duration, segments from each femur were heated at 500 degrees C and 900 degrees C for 15, 30, and 45 minutes. Trace and major elements, percent weight nitrogen and carbon, and color differences were measured. Of the chemical elements that could be reliably examined, cobalt, chromium, nickel, zinc, strontium, lead, rubidium, uranium, phosphorus, percent weight nitrogen and carbon, and all color points exhibited differences between temperatures. Differences between duration times at 500 degrees C were found in zinc, uranium, and percent weight nitrogen and carbon. At 900 degrees C, changes in heat duration impacted values for zinc, lead, rubidium, and percent weight nitrogen and carbon. Fluorescence was collected using a spectrofluorimeter with excitation in the ultraviolet range (300 -395 nm) and emission in the visible range (400 to 700 nm). Emission spectra at 365 nm excitation, the wavelength of most handheld UV lights, were consistent for collagen for samples heated at 0 degrees C and 100 degrees C, with no fluorescence at 300 degrees C and 500 degrees C. At 700 degrees C and 900 degrees C, fluorescence intensity increased from 640 nm to 700 nm, for which the underlying mechanism is as yet unknown. Parallel factor analysis of the temperature dataset indicated a two component model appropriately describes the excitation emission matrices. The first component of the model is an unknown fluorophore (excitation = 330 nm, emission = 696 nm), resulting in red fluorescence. The second component (excitation = 365 nm, emission = 458 nm) results in a blue fluorescence corresponding to the collagen and organic components well-documented in the literature.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Traci L Van Deest.
Thesis:
Thesis (Ph.D.)--University of Florida, 2014.
Local:
Adviser: WARREN,MICHAEL WADE.
Local:
Co-adviser: KRIGBAUM,JOHN.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2016-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0046634:00001