Renal Nitric Oxide, Pro- and Anti-Oxidant Pathways in Rat Pregnancy and Malignant Hypertension

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Material Information

Title:
Renal Nitric Oxide, Pro- and Anti-Oxidant Pathways in Rat Pregnancy and Malignant Hypertension
Physical Description:
1 online resource (163 p.)
Language:
english
Creator:
Cunningham, Mark W
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Medical Sciences, Physiology and Pharmacology (IDP)
Committee Chair:
BAYLIS,CHRISTINE
Committee Co-Chair:
SAYESKI,PETER PAUL
Committee Members:
CONRAD,KIRK PETER
SEGAL,MARK STUART

Subjects

Subjects / Keywords:
hypertension -- nitricoxide -- oxidativestress -- pregnancy -- renal
Physiology and Pharmacology (IDP) -- Dissertations, Academic -- UF
Genre:
Medical Sciences thesis, Ph.D.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Nitric Oxide (NO) bioavailability is important for facilitating the normal renal hemodynamic changes that occur during a normal pregnancy and blood pressure regulation. During pregnancy renal NO production dilates both the afferent and efferent arterioles of the renal vasculature which decreases renal vascular resistance (RVR) and thus increases renal blood flow (RBF) and (GFR). Normal pregnancy involves increased renal sodium reabsorption, metabolism, and oxygen consumption, which can cause increased oxidative stress. Oxidative stress can decrease in nitric oxide (NO) bioavailability and cause pregnancy complications, such as preeclampsia. We attempted to generate a at model of preeclampsia by feeding Sprague-Dawley (SD) rats a pro-oxidant western diet with angiotensin II (ANGII) and tumor necrosis factor alpha (TNF alpha) during the last 14 days of pregnancy. Furthermore, an increase oxidative stress, over-activation of the renin-angiotensin-aldosterone-system (RAAS), and impaired nitric oxide (NO) bioavailability are major players in the pathogenesis of hypertension. One animal model of hypertension caused by an activation of the RAAS system is the Cyp1a1-Ren2 transgenic rat (TGR). Although these rats suffer from massive increases in blood pressure over a ten day period, their kidney function, measured by GFR, remains intact. We tested the hypothesis that the maintained GFR in the Cyp1a1-Ren2 TGRs is due to preservation of the intrarenal NO and antioxidant system. Thus the purpose of this current body of work is to examine the renal NO and oxidative stress biomarkers during a normal rat pregnancy, a rat pregnancy compromised by oxidative stress and inflammation, and in Cyp1a1-Ren2 transgenic rats (TGR) with ANG II-dependent malignant hypertension. The results of our study showed that pregnant rats are protected against renal oxidative stress , hypertension, and any other pregnancy complications given a pro-oxidant western diet with ANGII and TNF alpha. The lack of an increase in intrarenal oxidative stress along with increased/maintained renal NO bioavailability play a role in maintaining normal renal hemodynamics in Cyp1a1-Ren2 transgenic rats (TGR) with ANG II-dependent malignant hypertension.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Mark W Cunningham.
Thesis:
Thesis (Ph.D.)--University of Florida, 2014.
Local:
Adviser: BAYLIS,CHRISTINE.
Local:
Co-adviser: SAYESKI,PETER PAUL.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2014-11-30

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0046616:00001