Developmental Programming of the Preimplantation Bovine Embryo by Colony Stimulating Factor 2

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Material Information

Title:
Developmental Programming of the Preimplantation Bovine Embryo by Colony Stimulating Factor 2
Physical Description:
1 online resource (178 p.)
Language:
english
Creator:
Dobbs, Kyle B
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Animal Molecular and Cellular Biology
Committee Chair:
HANSEN,PETER J
Committee Co-Chair:
DRURY,KENNETH C
Committee Members:
WOHLGEMUTH,STEPHANIE
RESNICK,JAMES LEWIS

Subjects

Subjects / Keywords:
bovine -- csf2 -- embryo
Animal Molecular and Cellular Biology -- Dissertations, Academic -- UF
Genre:
Animal Molecular and Cellular Biology thesis, Ph.D.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
The maternal environment is an important determinant of preimplantation development of the mammalian embryo. Many actions of the mother are mediated by alterations in secretion of regulatory molecules called embryokines produced by the endometrium. One of these molecules, colony stimulating factor 2 (CSF2), causes several actions on the embryo when added from days 5-7 of development including altering lineage commitment of the inner cell mass (ICM) and trophectoderm (TE) of the blastocyst, increasing competence of an embryo to become a blastocyst and to establish pregnancy after transfer. Multiple experiments were carried out to understand the mechanisms of CSF2 on the development of the preimplantation bovine embryo. First, experiments were conducted to evaluate regulation by CSF2 of pluripotency within the ICM and TE differentiation and growth. The proportion of isolated ICM colonies that survived until the end of the first cellular passage was greater for ICM derived from blastocysts produced in the presence of CSF2 than ICM from control blastocysts. There was little effect of CSF2 on characteristics of TE outgrowths from blastocysts. In addition, actions of CSF2 were found to occur through a signaling pathway that is likely independent of CSF2RB because CSF2RB was undetectable from the zygote to blastocyst stages. Second, the actions of CSF2 from day 5-7 of development on characteristics of the embryo at day 15 of gestation to determine whether CSF2 causes long-term changes in embryonic development. CSF2 decreased length and IFNT secretion of female embryos at Day15 but increased length and IFNT secretion of male embryos. Similarly, CSF2 affected the transcriptome and methylome differently for female embryos than male embryos. Finally, immunofluorescent labeling with anti-5- methylcytosine was used to evaluate global DNA methylation during preimplantation development. Results suggest that DNA methylation undergoes dynamic changes during the preimplantation period in a manner that is dependent upon gender and that the TE of the blastocyst is more methylated than the ICM. There was no effect of CSF2 on global DNA methylation at the blastocyst stage. Overall, these results establish a mechanism by which the maternally secreted embryokine, CSF2, alters the developmental trajectory of the bovine preimplantation embryo.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Kyle B Dobbs.
Thesis:
Thesis (Ph.D.)--University of Florida, 2014.
Local:
Adviser: HANSEN,PETER J.
Local:
Co-adviser: DRURY,KENNETH C.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2015-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0046537:00001