Detection Methods and Taxonomic Clarifications for Three Emerging Tree Pathogens in Florida, Diplodia Corticola, Diplodi...

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Material Information

Title:
Detection Methods and Taxonomic Clarifications for Three Emerging Tree Pathogens in Florida, Diplodia Corticola, Diplodia Quercivora and Raffaelea Lauricola
Physical Description:
1 online resource (80 p.)
Language:
english
Creator:
Dreaden, Tyler J
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Forest Resources and Conservation
Committee Chair:
SMITH,JASON ANDREW
Committee Co-Chair:
DAVIS,JOHN MARK
Committee Members:
SOLTIS,PAMELA S
PLOETZ,RANDY C
WINGFIELD,MICHAEL J

Subjects

Subjects / Keywords:
corticola -- diplodia -- lauricola -- quercivora -- raffaelea
Forest Resources and Conservation -- Dissertations, Academic -- UF
Genre:
Forest Resources and Conservation thesis, Ph.D.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Laurel wilt of redbay (Persea borbonia) and other Lauraceae, caused by Raffaelea lauricola, and cankers of live oak (Quercus virginiana), caused by Diplodia corticola and D. quercivora, affect the health of trees in Florida. A phylogenetic study of Raffaelea and related taxa revealed that, as currently defined, the genus is not monophyletic and that Raffaelea and Grosmannia need to be reevaluated. Two methods to detect R. lauricola were developed. One utilizes the SSU locus and qPCR, is not taxon specific, but is useful experimentally. Another uses multiple taxon-specific loci and PCR, reduces diagnostic time from 1 week to a day, and can be used to diagnose laurel wilt. A PCR-RFLP-based method detects and differentiates D. corticola and D. quercivora. Using this method, a survey of isolates recovered from symptomatic live oaks confirm D. corticola in Duval, Highlands, Levy, Polk, and Washington Counties, and D. quercivora in Alachua, Duval, and Marion Counties in Florida.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Tyler J Dreaden.
Thesis:
Thesis (Ph.D.)--University of Florida, 2014.
Local:
Adviser: SMITH,JASON ANDREW.
Local:
Co-adviser: DAVIS,JOHN MARK.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2015-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0046535:00001