Effect of Triclosan on the Global Gene Expression in the Late Gestation Ovine Placenta

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Material Information

Title:
Effect of Triclosan on the Global Gene Expression in the Late Gestation Ovine Placenta
Physical Description:
1 online resource (36 p.)
Language:
english
Creator:
Grapes, Ashley L
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Master's ( M.S.)
Degree Grantor:
University of Florida
Degree Disciplines:
Animal Molecular and Cellular Biology
Committee Chair:
WOOD,CHARLES E
Committee Co-Chair:
HANSEN,PETER J
Committee Members:
KELLER WOOD,MAUREEN
BAKER,HENRY V

Subjects

Subjects / Keywords:
edc -- microarray -- pregnancy -- triclosan
Animal Molecular and Cellular Biology -- Dissertations, Academic -- UF
Genre:
Animal Molecular and Cellular Biology thesis, M.S.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
This study was designed to test the hypothesis that the antibacterial agent,triclosan, would cause global gene expression changes similar to estradiol. We chronically catheterized pregnant ewes at 120 days of gestation by placing polyvinyl catheters into the femoral veins and arteries of the fetus and mother.After the five-day recovery period, each fetus was subjected to a continuous two-day infusion of either 200micrograms/kg triclosan (n=6), 500micrograms/kg estradiol (n=4),or saline (n=5). After the infusion period, the animals were sacrificed and the fetal tissues were collected, including the placental cotyledons. An Agilentovine array containing 15,744 probes and annotated with homologous human protein names was used for global transcriptomic analysis of the placenta.Compared to the control, triclosan and estradiol differentially expressed 957and 895 genes, respectively (P 0.05). Of these genes, the two treatment groups only differentially expressed 7.4% (128) of the same genes, signifying a potent non-estrogenic effect of triclosan. Enriched biological pathways were determined using the annotated clustering tool provided by the Database for Annotation, Visualization and Integrated Discovery(DAVID) website.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Ashley L Grapes.
Thesis:
Thesis (M.S.)--University of Florida, 2014.
Local:
Adviser: WOOD,CHARLES E.
Local:
Co-adviser: HANSEN,PETER J.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2016-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0046389:00001