Phenotypic and Genotypic Variation in Bacterial Spot Causing Xanthomonads

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Material Information

Title:
Phenotypic and Genotypic Variation in Bacterial Spot Causing Xanthomonads
Physical Description:
1 online resource (110 p.)
Language:
english
Creator:
Timilsina, Sujan
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Master's ( M.S.)
Degree Grantor:
University of Florida
Degree Disciplines:
Plant Pathology
Committee Chair:
VALLAD,GARY EDWARD
Committee Co-Chair:
JONES,JEFFREY B
Committee Members:
GOSS,ERICA M

Subjects

Subjects / Keywords:
xanthomonas
Plant Pathology -- Dissertations, Academic -- UF
Genre:
Plant Pathology thesis, M.S.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Bacterial spot of tomato, a disease caused by fourphylogenetically distinct xanthomonads, is a major concern for tomato productionaround the world. Extensive use of copper-based bactericides has resulted in thewidespread occurrence of copper tolerant strains of xanthomonads in tomato productionareas. In 2011-2012, 176 Xanthomonas strainswere sampled from tomato production areas throughout Florida. All strains were identifiedas X. perforans tomato race 4, of which all strains but one werecharacterized as copper tolerant. Bacteria were characterized for theirsensitivity against streptomycin, kasugamycin and quinoxyfen. A total of 32%strains were streptomycin resistant and 86% of the strains from transplanthouse were resistant to the antibiotic. Kasumin (a.i. 2.3% kasugamycin) wasfound effective against bacteria while quinoxyfen had no bactericidal effect onits own but significantly reduced bacterial population in combination withcopper in select strains. Genomic sequencingof 16 representative strains from the survey along with possible recombinantstrain Xp2010 was analyzed for their effectorprofiles. Phylogenetic analysis of the 11 core effectors revealed that fiveFlorida strains varied in XopQ effector from X. perforans 91-118 butwere identical to the strains Xp2010 and X. euvesicatoria 85-10. Furthermore,the Florida strains lacked XopAF gene but XopJ4 effector was presentcorresponding to tomato race 4 for these strains. We also studiedvariation in Xanthomonas species responsible for BLS in tomato usingstrains collected from around the world and sequenced six housekeeping genesfor each strain. Phylogenetic analysis of these Xanthomonas strainscausing bacterial spot of tomato revealed highly conserved nature of X.gardneri species, two different clusters within X. vesicatoria andexchange of genetic material between X. euvesicatoria and X.perforans. Further efforts will be focused in understanding the pattern ofrecombination and analyzing the type III effector profiles in the bacterialspot causing xanthomonads population.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Sujan Timilsina.
Thesis:
Thesis (M.S.)--University of Florida, 2013.
Local:
Adviser: VALLAD,GARY EDWARD.
Local:
Co-adviser: JONES,JEFFREY B.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2015-12-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2013
System ID:
UFE0046351:00001