Strawberry Flavor

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Material Information

Title:
Strawberry Flavor from Genomics to Practical Applications
Physical Description:
1 online resource (195 p.)
Language:
english
Creator:
Chambers, Alan H
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Horticultural Sciences
Committee Chair:
FOLTA,KEVIN M
Committee Co-Chair:
WHITAKER,VANCE M
Committee Members:
KLEE,HARRY JOHN
MOU,ZHONGLIN

Subjects

Subjects / Keywords:
anthranilate -- decalactone -- fanes1 -- flavor -- fragaria -- gamma -- linalool -- methyl -- strawberry -- volatile
Horticultural Sciences -- Dissertations, Academic -- UF
Genre:
Horticultural Sciences thesis, Ph.D.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Strawberry (Fragariax ananassa) flavor has been studiedfor a century, and poor flavor quality has been lamented for about the sametime (Fletcher, 1917).  Many papers have documented the components ofstrawberry flavor (sugars, acids, texture, volatiles, etc), but catalogingfailed to make flavor genetically tractable. The work herein demonstrates the effectiveness of combining a RNAsequencing genomics approach with volatile phenotyping in a segregatingpopulation to identify functional gene candidates.  This approach enabled the discovery of thesingle, dominant gene conferring production of gamma-decalactone.  A functional molecular marker was thencreated that faithfully cosegregates with the volatile phenotype in cultivarsoutside the original population and even for a set of wild Fragaria genotypes.  Results are also presented for other distinctstudies.  The causal allele for linaloolproduction, FaNES1, was tested indiverse Fragaria accessions and wasonly found in octoploids.  This suggeststhat the FaNES1 flavor allele aroseshortly after octoploidization.  Ahypothesis was developed for linalool-positive genotypes that lack the FaNES1 allele.  Another study was inspired by industry demandfor accurate cultivar identification.  Amultiplexed, SSR-based fingerprinting platform was developed to accuratelyidentify strawberry cultivars.  Thefingerprinting platform efficiently resolved differences between over 200genotypes and even between full siblings. Finally, results are presented that show the efficacy and relativepotency of gamma-decalactone and methyl anthranilate against strawberry fungal,oomycete, and bacterial pathogens. Approximately 1-5 millimolar of either volatile was generally sufficientfor complete control of pathogen growth using both in vitro and pseudo-in vivoassays.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Alan H Chambers.
Thesis:
Thesis (Ph.D.)--University of Florida, 2013.
Local:
Adviser: FOLTA,KEVIN M.
Local:
Co-adviser: WHITAKER,VANCE M.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2014-12-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2013
System ID:
UFE0046096:00001