Effects of a Dietary Restriction plus Exercise Program on Central Adiposity in Obese Older Women

MISSING IMAGE

Material Information

Title:
Effects of a Dietary Restriction plus Exercise Program on Central Adiposity in Obese Older Women
Physical Description:
1 online resource (59 p.)
Language:
english
Creator:
Karabetian, Christy
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Master's ( M.S.)
Degree Grantor:
University of Florida
Degree Disciplines:
Psychology, Clinical and Health Psychology
Committee Chair:
Anton, Stephen D
Committee Members:
Dotson, Vonetta M
Mccrae, Christina Smith
Boggs, Stephen R

Subjects

Subjects / Keywords:
aging -- obesity
Clinical and Health Psychology -- Dissertations, Academic -- UF
Genre:
Psychology thesis, M.S.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Aging is associated with an increase in central adiposity and an accelerated loss of muscle mass. These body composition changes can increase risk of functional decline and ultimately disability in older adults. The current study tested whether a weight loss intervention in which dietary restriciton was combined  with a multi-component exercise program could reduce central fat depots (i.e., visceral and subcutaneous adipose tissue)  in obese older women. Twenty-five obese older women (age range = 55–79 years) with mild to moderate physical impairments were randomly assigned to a dietary restriction plus exercise (DR+E) intervention group or successful aging educational control for 24 weeks.  Participants in the DR+E group attended weekly weight management sessions, as well as three structured exercise sessions each week. Participants in the successful aging educational control attended monthly health education lectures. Visceral and subcutaneous adipose tissues within the abdominal region were measured by magnetic resonance imaging, and analyses of covariance were conducted to examine changes in volume from baseline to post-treatment.  Relationships between changes in central adiposity, body weight, and physical function were also examined. Participants in the DR+E group had a significantly greater reduction in visceral adipose tissue compared to participants in the successful aging group (-1057.4 ± 347.0 cm3 vs. 199.9 ± 378.2 cm³; p 3 vs. 1096.6 ± 1406.9 cm3, p = .05). There was a strong, positive correlation between changes in body weight and subcutaneous adipose tissue (r = .57, p
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Christy Karabetian.
Thesis:
Thesis (M.S.)--University of Florida, 2013.
Local:
Adviser: Anton, Stephen D.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2014-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2013
System ID:
UFE0045592:00001


This item is only available as the following downloads:


Full Text

PAGE 1

1 EFFECTS OF A DIETARY RESTRICTION PLUS EXE RCISE PROGRAM ON CENTRAL ADIPOSITY IN OBESE OLDER WOMEN By CHRISTY KARABETIAN A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE R EQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2013

PAGE 2

2 2013 Christy Karabetian

PAGE 3

3 For my parents.

PAGE 4

4 ACKNOWLEDGMENTS Special thanks go to Dr. Stephen Anton for his endless support and encouragement. Many thanks go to the members of my supervisory committee, Dr. Stephen Boggs, Dr. Christina McCrae, and Dr. Vonetta Dotson, for their time and assistance. Thanks go to my colleagues in the University of Florida Aging and Rehabilitation Rese arch Center and Weight Management Lab Finally, much gratitude and love go to my family and friends

PAGE 5

5 TABLE OF CONTENTS page ACKNOWLEDGMENTS ................................ ................................ ................................ .. 4 LIST OF TABLES ................................ ................................ ................................ ............ 7 LIST OF FIGURES ................................ ................................ ................................ .......... 8 LIST OF ABBREVIATIONS ................................ ................................ ............................. 9 ABSTRACT ................................ ................................ ................................ ................... 10 CHAPTER 1 INTRODUCTION ................................ ................................ ................................ .... 12 The Obesity Epidemic ................................ ................................ ............................. 12 Demographic Variations in Obesity Prevalence ................................ ............... 13 Age Related Changes in Body Composition ................................ .................... 14 Functional De cline in Obese Older Adults ................................ ........................ 17 Guidelines for the Treatment of Obesity ................................ ................................ 19 Weight Loss for Obese Older Adults ................................ ................................ 19 Lifestyle Treatment Approach ................................ ................................ ........... 20 Rationale for Current Stu dy ................................ ................................ .................... 23 Specific Aims and Hypotheses ................................ ................................ ............... 25 2 MATERIALS AND METHODS ................................ ................................ ................ 26 Research Methods and Procedures ................................ ................................ ....... 26 Participants ................................ ................................ ................................ ....... 26 Procedure ................................ ................................ ................................ ......... 28 Measurements ................................ ................................ ................................ .. 30 Statistical Analyses ................................ ................................ .......................... 32 3 RESULTS ................................ ................................ ................................ ............... 34 Sample Characteristics ................................ ................................ ........................... 34 Weight Loss and Physical Function ................................ ................................ ........ 34 Central Adiposity ................................ ................................ ................................ ..... 35 Changes in Body Weight and Central Adiposity ................................ ..................... 35 Changes i n Central Adiposity and Physical Function ................................ .............. 36 Changes in Body Weight and Physical Function ................................ .................... 36 4 DISCUSSION ................................ ................................ ................................ ......... 40 Summary and Future Directions ................................ ................................ ............. 40

PAGE 6

6 Strengths and Limitations ................................ ................................ ....................... 43 Conclusions ................................ ................................ ................................ ............ 45 LIST OF REFERENCES ................................ ................................ ............................... 46 BIOGRAPHICAL SKETCH ................................ ................................ ............................ 59

PAGE 7

7 LIST OF TABLES Table page 3 1 Participant Demographics and Baseline Characteristics ................................ .... 37

PAGE 8

8 LIST OF FIGURES Figure page 3 1 Mean changes in VAT volume between groups after 24 weeks of intervention. ................................ ................................ ................................ ........ 38 3 2 Mean changes in SAT volume between groups after 24 weeks of intervention. ................................ ................................ ................................ ........ 39

PAGE 9

9 LIST OF ABBREVIATIONS BMI Body Mass Index DR+E Dietary Restriction plus Exercise SAT Subcutane ous adipose tissue SPPB Short Physical Performance Battery VAT Visceral a dipose tissue

PAGE 10

10 Abstract of Thesis Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Maste r of Science EFFECTS OF A DIETARY RESTRICTION PLUS EXE RCISE PROGRAM ON CENTRAL ADIPOSITY IN OBESE OLDER WOMEN By Christy Karabetian May 2013 Chair: Stephen D. Anton Major: Psychology Aging is associated with an increase in central adiposity and an acc elerated loss of muscle mass These body composition changes can increase risk of functional decline and ultimately disability in older adults. The current study tested whether a weight loss intervention in which dietary restriciton was combined with a m ulti component exercise program could reduce central fat depots (i.e., visceral and subcutaneous adipose tissue) in obese older women. Twenty five ob ese older women (age range = 55 79 years ) with mild to moderate physical impairments were randomly assigne d to a dietary restriction plus exercise (DR+E) intervention group or successful ag in g educational control for 24 weeks Participants i n the DR+E group attended weekly weight management sessions, as well as three structured exercise sessions each week. P articipants in the successful aging educational control attended monthly health education lectures. Visceral and subcutaneous adipose tissues within the abdominal region were measured by magnetic resonance i maging and analyses of covariance were conducted to examine changes in volume from baseline to post treatment. Relationships between changes in central adiposity, body weight, and physical function were also examined Participants in the DR+E group had a

PAGE 11

11 significantly greater reduction in visceral adi pose tissue compared to participants in the successful aging group ( 1057.4 347.0 cm 3 vs. 199.9 378.2 cm ; p < .05), and significantly greater reduction in subcutaneous adipose tissue compared to participants in the successful aging group ( 2970.6 13 45.1 cm 3 vs. 1096.6 1406.9 cm 3 p = .05 ). There was a strong, positive correlation between changes in body weight and subcutaneous adipose tissue (r = .57, p < .01 ); however, there were no other significant associations between changes in body weight, a diposity, or physical function. Our findings indicate that a lifestyle intervention involving moderate reductions in dietary intake combined with a multi component physical activity program can produce significant reductions in central fat depots in obese older women.

PAGE 12

12 CHAPTER 1 INTRODUCTION The Obesity Epidemic By the year 2050, the number of older adults living in the United States is expected to double from 40.2 million to 88.5 million ( 1 ). This trend taken together with the increasing prevalence of obesity, is alarming as approximately two thirds of older adults (age 60 years and older) are overweight and one third of older adults are obese ( 2 ). Thus, the current shift in the age distribution of the American population may increase the number of obes e older adults even without an increase in obesity prevalence ( 1 ). behaviors associated with physical health. Genetics, metabolic regulation, environmental factors, culture, and socioeconomic status all impact body w eight throughout the lifespan ( 3 ). Obesity is caused by an imbalance in energy intake and expenditure, resulting in a state of prolonged positive energy balance ( 4 ). B ehavioral and environmental factors (ex., foo d i ntake and sedentary lifestyle) have largely con tributed to the increasing p revalence of obesity Recently, an analysis of four nationally representative surveys of food intake in the U.S. population consisting of 19 years), identified that average daily energy intake has increased from 1,765 kcal/day in 1977 78 to 2,164 kcal/day in 2003 06 ( 5 ). Concurrently, data from the National Health Interview Survey indicates that approximately 40% of adults do not participation in any physical activity behaviors ( 6 ) Excess body weight reaching obesity, classified based on the measurement of body mass index (BMI) greate r than 30 kg/m 2 is associated with increased risk of total

PAGE 13

13 mortality ( 7 ) More than 400,000 deaths per year are attributed to obesity ( 8 ). Many older adults are obese and this is associated with increased morbidity and reduced quality of life. Obesity h as been clearly established as a risk factor for many chronic health conditions in older adults such as diabetes mellitus, hypertension, cardiovascular disease, osteoarthristis, and certain cancers ( 9 ). Excess weight also accelerates the development of ost eoarthritis ( 10 ). In addition, it can be accompanied by pain and suffering, reduced quality of life, and medical costs for treatment ( 11 13 ) Demographic Variations in Obesity Prevalence Considerable subgroup variation exists in the prevalence of obesity a ccording to age sex, and race/ethnicity According to NHANES 2009 2010 data, obesity prevalence do not differ between men and women ( 14 ) Examination of ethnic/racial subgroups reveals trends that indicate there are higher rates of obesity among some subg roups which may be the result of factors such as genetics, education, environment, or cultural practices ( 15 ). Across the lifespan, nearly half of African American females are obese and significantly different from African American males and Caucasian fem ales ( 16 ). Moreover, the highest rates of overweight, as def ined by a BMI greater than 25 29.9 kg/m 2 are observed among non Hispanic, African American females between the ages of 40 to 59 years of age (81.5%) and 60 years of age and older (81.7%) and the highest rates of obesity are observed among non Hispanic, African American females between the ages of 40 to 59 years of age (53.2%) ( 16 ). Thus, obese older African Americ an women represent a particularly high risk population Obesity in Older Adults The prevalence of obesity is increasing in older adults ( 17 ). As in younger adults, obesity in older adults is associated with increased risk of morbidity ( 18 ). Some evidence suggest that high body weight may not be an important

PAGE 14

14 adverse factor for mortality i n older adults and an ideal BMI appears to be higher in older than in younger adults ( 19 ) Based on NHANES (1999 to 2002) data, however an estimated 25% of excess deaths in individuals over 70 years old are attributed to obesity ( 20 ). Notably, the relatio nship between mortality and BMI in older adults may be misleading since variations in body composition (i.e., lean mass vs. fat mass) are not captured by BMI With aging, there is an increase in fat mass and a decrease in lean body mass ( 21 ). These body c omposition changes can lead to older adults having more body fat compared to younger adul ts who have the same BMI. Age Related Changes in Body Composition Body composition changes in older age as fat free mass decreases while fat mass increases and gets redistributed in the abdominal area ( 22 24 ). These changes in body composition (i.e., muscle loss and fat gain) can occur independently from changes in body weight and BMI ( 25 ) and are accelerated after middle age especially during menopause ( 21 ). Contro lling for BMI, women and older adults have a higher percent body fat than men and younger individuals ( 26 ). Therefore, central adiposity may be more important in assessing risk than total body fat ness especially in older adults. Sarcopenia Normal aging is associated with physiological changes that result in functional decline A fter the age of 50 years, the rate of lean tissue mass loss is estimated to be approximately 1% to 2% annually ( 23 ) This reduction of muscle mass, strength, and quality with ag ing, can lead to sarcopenia defined as poverty of flesh describing age related loss of muscle mass ( 27 ), and the decline of physical function. Some estimates indicate that approximately one quarter to one half of adults aged 65 and older are sarcopenic ( 28 ), though the true prevalence is difficult to ascertain given the lack of a universally accepted definition of sarcopenia. While loss of muscle mass

PAGE 15

15 explains a significant component of weakness, s everal other mechanisms may underlie and contribute to t he accelerated rate of muscle loss and strength observed in older adults Some potential mechanisms contributing to functional decline (or sarcopenia) likely include hormonal changes ( 29 ), low levels of physical activity ( 30 31 ), and fat accumulation with in the muscle ( 32 ). In addition, o besity has been identified as important contributor to the progressio n of sarcopenia ( 33 34 ) due to the accumulation of adipose tissue and subsequent increases in both cortisol and pro inflammatory cytokines ( 35 ). These physiological changes can promote abdominal fat accumulation, the development of insulin resistance, and skeletal muscle atrophy ( 36 37 ) Increased adiposity combined with lower skeletal muscle influences the development of functional decline ( 38 ). A key determinant of functional abilities is the relative relationship of muscle strength to body mass ( 39 41 ). Evidence suggests that obese older adults typically possess a reduced strength to body mass ratio compared with non obese older adults. Relative mus cle mass has recently been reported to decrease by 0.02 kg/year for every standard deviation increase in fat mass (i.e., SD = 7.1 kg for ma les and SD = 9.1 kg for females ) (42) In a cross sectional study of postmenopausal women participants who were ove rweight or obese showed that a 10 kg increase in fat mass was significantly associated with lower physical performance, lower physica l activity, and higher frequency of impairments in activities of daily living ( 43 ). Thus, obesity contributes to reducing m uscle mass beyond the progressive physiological changes associated with normal aging and may accelerate the rate of functional decline

PAGE 16

16 Central Adiposity. Central obesity is determined by the accumulation of both subcutaneous adipose tissue (SAT) and vis ceral adipose tissue (VAT) in the abdominal region. Visceral adipose tissue is metabolically active ( 34 ), making the excess accumulation of VAT particularly hazardous in the pathogenesis of metabolic abnormalities ( 44 ) and cardiovascular disease ( 45 ). Vi sceral adipose tissue, located in the body cavity beneath the abdominal muscles, secrets adipokines (e.g., TNF 6) and other protein molecules, and promotes a pro inflammatory state which interferes with metabolic regulation ( 22, 46 47 ). This inte rference can lead to insulin resistance ( 48 50 ), hyperinsulinemia and hyperglycemia ( 48, 51 ), high blood pressure ( 52 ), metabolic syndrome ( 53 ), and non alcoho lic fatty liver disease ( 54 ). In fact, visceral adipose tissue is a stronger predictor of metabol ic alterations than total body fat percentage ( 49 ). In contrast to VAT, there is little evidence that SAT is associated with risk of pathology and may even have a protective effect on metabolic parameters ( 55 ). Inflammation may contribute to the developmen t of diseases such as atherosclerosis ( 56 ) autoimmune disease ( 57 ) and c ertain c ancer s ( 58 ). Central adiposity is a stronger risk factor for morbidity and mortality than BMI, total body fat, or other sites of fat predominance ( 49, 59 60 ). The relative ri sk of death imposed by central adiposity for vascular diseases reaches a risk equivalent to that reported for hypercholesterolemia, hypertension, and smoking ( 49 ). In obese postmenopausal women, atherogenic levels of lipids and lipoproteins were independe ntly related to central adiposity ( 61 ) Consistent with the subgroup variations in the prevalence of obesity, difference in body fat distribution can be seen by age sex, and race/ethnicity independent of BMI

PAGE 17

17 Studies indicate that older males have more a dipose tissue within the abdominal cavity and less SAT compared to middle aged males ( 62 ) and age is positively correlation with VAT in females ( 63 ). African American women have higher rates of central adiposity compared to African American men and Caucasi ans ( 64 66 ). Among older adults, t he relationship between inflammation and increased risk of disability has been established ( 67 68 ). As pro inflammatory cytokines promote systemic inflammation and progressive destruction of tissue these protein molecule s have a direct effect on physical function by accelerating body composition changes ( 67, 69 ) and impact cognitive function by accelerating brain atrophy typical of the aging process ( 70 71 ). Functional Decline in Obese Older Adults As the population ages, the risk of physical and/or cognitive impairments secondary to obesity and related lifestyle behaviors threatens the health, safety and quality of life in older adults. I f current obesity trends continue, there will also be an increase in chronic illnes s in an otherwise already vulnerable population. Physical function, a marker of performance in domains such as activities of daily living and mobility tasks, is a strong determinant of independent living and health ( Guralnik & Simonsick, 1993 ). As such, t h e definition of disability was expanded in the International Classification System of Functioning, Disability, and Health (ICF) to include functional impairments (e.g., deficits in strength or cardiorespiratory fitness) along with a range of behaviors incl uding either discrete tasks/actions (i.e., walking tests, stair climbing, and balance tests) or participation in life situations ( 73 ). Physical impairments and disabilities in older adults can lead to a loss of independence ( 74 ), increased use of

PAGE 18

18 support s ervices ( 75 ), hospitalization ( 76 77 ), and mortality (78 79 ). Compared to adults with a normal body weight, obese o lder adults experience impairments in basic activities of daily living approximately five years earlier and are twice as likely to develop fu nctional impairments and/or activities of dai ly living disabilities ( 80 ). In addition, cognitive decline has been linked to disability onset in older adults ( 81 ). Several cross sectional studies suggest a negative relationship between obesity and cognitive function in older adults ( 82 84 ). Thus, o besity places older adults at high risk for the development of disability (80) Physical Function. The relationship between obesity and physical function has been widely examined ( 38, 85 88 ) and data show that hig h body weight and high BMI are associated with increased risk for functional impairment and disability. Furthermore, the relationship between body composition and physical function indicates that fat mass, but not fat free mass, is more predictive of funct ional limitations ( 42 89 ). Several studies suggest that central adiposity, measured by waist circumference, may adversely impact mobility ( 9 0 91 ); however, the results of these studies are limited by their use of a n anthropomorphic index measure of body fat distribution and subjective measure s of physical function (i.e., self report assessment tools ) Therefore, the relationship between central adiposity and physical function is not well understood and should be examined using objective functional assessm ent tools and site specific quantification of visceral and subcutaneous adipose tissue. Cardiac Function Impairments in c ardiac function in obese patients is attributed to excess body weight and duration of obesity ( 92 ), and there is evidence that viscera l fat accumulation is a specific risk factor for metabolic abnormalities ( 93 96 ) that can

PAGE 19

19 lead to cardiovascular and metabolic disease ( 9 7 100 ). Cardiovascular disease cerebrovascular disease, and diabetes mellitus are among the top ten leading causes of death in older adults living in the U.S. ( 101 ). Notably, as survival rates are increasing ( 102 ) individuals are l iving wide range of disabilities including motor disorders, memory loss, sensory problems and depression ( 103 ). Guidelines for the Treatment of Obesity Weight Loss for Obese Older Adults According to the National Institute of Health ( 2000 ) clinical guidelines for the treatment of overweight and obesity in adults, weight loss is recommended for individuals with a BMI equal to or greater than 30 kg/m 2 and for individuals with a BMI between 25 and 29.9 kg/m 2 with two or more risk factors including hypertension, cigarette smoking, high low density lipoprotein cholesterol, impaired fasting glucose, family history of early cardiovascular disease, and age (male 45 years, females 55 years) (4) The recommendation for weight loss in older adults has been controversial. Several longitudinal observational studies show that weight loss i n older adults i s associated with increased mortality ( 104 106 ) ; however, most observational studies often fail to disting uish intentional weight loss (e.g ., to promote health) a nd unintentional weight loss (e.g ., as a precursor to illness). Additionally, some critics argue that weight loss in this population can accelerate the los s of lean body mass that occurs with aging ( 107 ) Despite these claims, The American Society of Nutrition and the North American Association for the Study of Obesity ( 4,17 ) both recommend intentional weight loss for older adults only in the case of obesity combined with weight related comorbidities or functional limitations. Evidence supports that a r eduction in body weight, even if modest

PAGE 20

2 0 (e.g., 5 10%), produce s beneficial effects on metabolic, cardiac, and physical function in obese older adults ( 108 111 ) Lifestyle Treatment Approach Lifestyle interventions represent the first line of intervention in the behavioral treatment of obesity ( 4 ). Lifestyle interventions consist of calorie restriction, physical activity, and behavioral modification with the goa l of creating a negative energy balance for weight loss. Caloric restriction of 500 to 1000 kcals below baseline intake is recommended for a weight loss of approximately 0.4 0. 9 k g per week. Elements of physical activity include aerobic training, resistanc e tr aining, and flexibility exercises. The 2008 Physical Activity Guidelines for Americans recommends at least 150 minutes of moderate intensity aerobic activity every week plus muscle strengthening activities on two or more days per week for older adults ( 6 ). Behavioral modification includes self monitoring, goal setting, social support, stimulus control, and relapse prevention. Benefits of lifestyle interventions often extend beyond weight loss to include improvements in hypertension, glucose intolerance hyperlipidemia, physical functioning, and quality of life ( 112 116 ). Studies to date indicate lifestyle interventions which combine dietary restriction and physical activity have favorable effects in obese older adults on changes in body weight, body com position, and measures of physical function. Body Weight. The ability of lifestyle interventions to produce clinically meaningful reductions in body weight in older adults has now been well established ( 17,108,113 115, 11 7 ). Research indicates that lifesty le modification programs combining a moderate energy deficit diet, increased physical activity, and behavior modification typically yield weight loss results of approximately 5 to 8.5 kg (5 to 10%)

PAGE 21

21 reduction of body weight after six months ( 118 ). Weight l oss of this magnitude has consistently been associated with clinically significant reductions in risk factors for cardiovascular disease, diabetes, and other health conditions ( 119 121 ). Body Composition. Lifestyle intervention s can produce significant re ductions in VAT and SAT in older obese adults ( 117,122 123 ). The combination of moderate energy deficit diet and increased aerobic e xercise training yielding a 4 9% body weight loss can directly reduce VAT ( 124 ) and even greater reductions can be seen when mean weight loss exceeds 10% reduction in body weight ( 125 ). Two randomized control trials demonstrate the benefit of a comprehensive lifestyle approach to reducing central adiposity. Santanasto and colleagues (2011) randomized obese older adults to a si x month physical activity program, with or withou t a weight loss intervention (123) Participants in the weight loss intervention lost significantly more body weight than participants in the physical activity alone group. The weight loss group also lost s ignificant amounts of total abdominal fat, visceral adipose tissue, and subcutaneous adipose tissue after six months, compared to no change in the physical activity alone group. Similarly, t he Diet, Exercise, and Metabolism for Older Women study ( 117 ), com pared the effects of caloric restriction alone versus caloric restriction plus aerobic exercise at a moderate or vigorous intensity in sedentary, obese postmenopausal wome n. After 20 weeks, t here was no significant difference in weight loss between group s; however, b oth abdominal visceral fat volume and subcutaneous fat volume decreased significantly among all groups Decreases in abdominal visceral fat were directly relat ed to the amount of weight lost.

PAGE 22

22 B oth calorie restriction and exercise induced weigh t loss can decrease VAT volumes in obese older adults ( 126 128 ). In a study in which obese older adults followed dietary restriction for six months without physical activity, results showed significant weight loss and a decrease in fat mass ( 129 ). Irwin a nd colleagues (2003) studied overweight and obese postmenopausal women randomized to an exercise (i.e., walking) or no exercise (i.e., stretching) control group (130) After 12 months, women in the exercise group showed significantly larger reductions in t otal body fat, VAT, and SAT, compared to women in the control group. Women who exercise for approximately 200 minutes per week reduced their body weight and percent of VAT without reducing their energy intake. This outcome illustrates a significant dose re sponse for greater body fat loss with increasing duration of exercise. Thus, examination of the literature on groups following dietary restriction with or without exercise shows similar weight loss, loss of body fat, and reductions in VAT ( 131 ). Given the concern for the loss of lean mass in older adults, adding exercise training to a diet ary restriction program helps preserve lean mass during weight loss (132 133 ). Beyond that, t he benefits of regular physical activity can also impact cardiometabolic heal th by reducing triglyceride levels, increas ing high density lipoprotein levels, reduc ing resting blood pressure, increas ing glucose tolerance, and reduc ing insulin resistance ( 134 ) Physical Function. Several studies indicate that physical function in obese older adults is improved following lifestyle intervention ( 108,113,115,135 ). Santanasto and colleagues (2011) examined physical function outcomes following six months of a physical activity program, with or without a weight loss intervention, in obese ol der

PAGE 23

23 adults (123) Significant improvements in physical function as measured by the Short Physical Performance Battery were achieved by the weight loss plus physical activity group only. The relationship between physical function and body composition was strongly inversely correlated with mean change in visceral adipose tissue and subcutaneous adipose tissue Rationale for Current Study Age related c hanges in body composition play an important role in functional decline and risk for disability. O besity ca n accelerate the progression of sarcopenia and physical disability in older adults ( 34,136 137 ). While some degree of sarcopenia is inevitable in older adults, contributing factors such as poor diet and sedentary lifestyle are modifiable and should be the focus of treatment for reducing obesity and functional decline As such dietary modifications and increased p hysical activity play an important role in maintaining lean muscle mass. However, the recommendation for weight loss in overweight and obese olde r adults has been controversial This controversy is due to concerns that weight loss can accelerate the loss of lean tissue that occurs with aging. Thus, t he potential risks and benefits of weight loss in obese older adults should be tested in the context of a lifestyle based treatment program that can help reduce body fat mass while retaining lean mass. To date there is some empirical support for the efficacy of lifestyle interventions on adaptive changes in physical function in obese older adults Few studies have explored the relationship between body fat distribution and physical function in obese older adults In most studies, surrogate measures of fat mass distribution (i.e., waist circumference) were used rather than direct measures of body fat dis tribution (i.e., duel energy x rays, magnetic resonance imaging, or computed

PAGE 24

24 tomography) to assess the association between body fat distribution and physical function ( 138 139 ) E fforts have been made to generate predictive values of simple anthropometri c measurements on regional body fat distribution. D ifferences between predicted and observed values of SAT were small; however, differences between predicted and observed values of VAT were large ( 140 ). T he re is limited information on the effect of lifesty le interventions on body composition, specifically the impact of treatment on reducing central fat depots (i.e. visceral and subcutaneous adipose tissue) in obese older adults. As body composition changes with aging, it is important to design interventions that can effectively reduce central adiposity, the fat depots that have been most associated with increased levels of pro inflammatory cytokines and also contribute to muscle atrophy in obese older adults. Although sex differences are not evident in the prevalence of obesity among older adults, i t is important to consider that sex difference s in body fat distribution have been noted independent of BMI. Even with equal BMI, women typically have a higher body fat percentage than men ( 141 ). In women, there is a shift in body composition around the time of menopause. A simultaneous acceleration in muscle loss paired with redistribution of fat to the central region plac es women at a particularly high risk for developing obesity related co morbidities Thus, ob ese older women represent a particularly important population for examining the effects of lifestyle interventions on weight loss, body composition, and physical functioning It remains critical to determine the best therapeutic approach for losing body f at, retaining muscle and bone mass, and prolonging functional mobility to extend quality of life throughout the lifespan in obese older women Therefore, m ore research is needed

PAGE 25

25 to examine the relationship between weight loss and regional body fat distribu tion, the effect of lifestyle treatments on central adiposity, and the role of central adiposity in maintaining physical function and delaying functional decline Specific Aims and Hypotheses This study has two specific aims. The first aim was to evaluate changes in central adiposity in obese older women following a six month lifestyle based weight loss intervention consisting of dietary restriction and a multi component exercise program. We hypothesized that as compared to the educational control group, t he Dietary Restriction plus Exercise group would produce greater reductions in both abdominal visceral adipose tissue volume and abdominal subcutaneous tissue volume The second aim was to examine the association between changes in body weight, regional f at distribution, and physical function. We hypothesized that among individuals randomized to the Dietary Restriction plus Exercise group, greater reductions in body weight would be associated with greater reductions in visceral adipose tissue volume and su bcutaneous adipose tissue volume Furthermore, we hypothesized that among individuals randomized to the Dietary Restriction plus Exercise group, g reater reductions in visceral adipose tissue volume and subcutaneous adipose tissue volume would be associated with improve ments in physical performance

PAGE 26

26 CHAPTER 2 MATERIALS AND METHODS Research Methods and Procedures The current study was a secondary analysis of data from a pilot, randomized controlled trial ( 142 ). The parent study was designed to demonstrate t he feasibility, acceptability, and efficacy of a weight loss program combined with moderate exercise in improving physical function in sedentary obese, older adult women with mild to moderate physical impairments The effects of a 24 week lifestyle based w eight loss plus comprehensive exercise intervention on body weight, physical function, and muscle strength were assessed and racial differences between Caucasian and African American women were examined. Participants Thirty four sedentary women between the ag es of 55 to 79 years old, with BMI > 28kg/m 2 who had mild to moderate physical impairment, were enrolled in the parent study A sedentary lifestyle was defined as engaging in less than 20 minutes per week of aerobic exercise over prior two months. Phy sical limitation was defined based on performance on the Short Physical Performance Battery (SPPB) with scores in the range of 4 to 10 indicating mild to moderate physical impairment. Participants were excluded for any of the following: body weight over 300lbs; weight loss of 10lbs within the past six months; history of surgery for weight loss; hospitalization within the past six months; significant underlying disease likely to limit lifespan and/or increase risk of interventions (cancer or any condition with a life expectancy < 5 years with the exception of non melanoma skin cancer; serious infectious diseases; myocardial infarction, cerebrovascular accident, or u nstable angina

PAGE 27

27 within the past six months; NYHA Class 3 or 4 congestive heart failure; aortic stenosis; chronic hepatitis; cirrhosis; kidney disease; solid organ transplantation; chronic gastrointestinal disorders; fibromyalgia; chronic fatigue syndrome; major psychiatric disorder); metabolic exclusions (i.e., resting blood pressure > 160/90 mmHg, fasting blood glucose > 160 mg/dl, fasting triglycerides > 400 mg/dl; patients on medication for hypertension, diabetes, or hyperlipidemia will not be excluded unless their values monoamine oxidase inhibitors; systemic corticosteroids; antibiotics for HIV or TB; chemotherapeutic drugs; or current use of prescription weight loss drugs); physical limitations likely to prevent exercise participation (i.e., use of walker; breathing pro blems that limit physical activity); conditions or behaviors likely to affect the conduct of the trial (e.g., unwilling or unable to give informed consent; unwilling to accept random assignment; likely t o move out of area within next two years; unable to a ttend weekly meetings; unwilling to complete paperwork; participation in another randomized research project; unwilling or unable to comply with study requirements or schedule); contraindications to MRI (MR incompatible implants or severe claustrophobia); c ontraindications to muscle biopsy (i.e., lidocaine allergy) Recruitment A total of 412 women responded to the study recruitment announcements. Three hundred and fifty eight women were excluded at the telephone screening phase and 17 individuals were e xcluded after completing the in person medical screening assessment. A total of 37 participants were eligible to participate in the study Of these, 34 participants were randomized to the DR+E intervention or educational control. The sample size included in the current study was 25 obese older

PAGE 28

28 women from the parent study. In total, nine participants were not included due to lack of post intervention follow up or inability to provide body composition measurements. Participants were recruited by the Aging a nd Rehabilitation Research Center, in conjunction with the Weight Management Lab, at the University of Florida from September 2006 to December 2008. Recruitment methods included direct mailings, solicitations at community based facilities, and the use of a Participant Registry maintained by the Institute on Aging at the University of Florida. All participants provided written informed consent and all protocols were approved by the I nstitutional R eview B oard (IRB 01) at the University of Florida. Procedure T his study was a single blinded, 24 week randomized clinical trial in which p articipants were randomly assigned to a comprehensive lifestyle intervention or to a successful aging education group. All groups meetings were held in a community based center (i. e., church facility). Dietary Restriction plus Exercise (DR+E) Group The intervention was designed to produce a modest weight loss of 7 10% of initial body weight. Participants met weekly for 60 minute group based weight management sessions and discussed topics related to behavior modification for weight loss, such as goal setting and self monitoring. At each weight management session, participants were provided with treatment plans including specific objectives for each meeting, particular methods to acco mplish the objectives, and illustrative handouts. Groups were co led by a registered dietician and a behavioral specialist. Adherence was monitored by weekly review of food diaries, physical activity logs, and weigh ins. Participants were provided with die tary restriction guidelines, calculated according to their self reported energy intake at

PAGE 29

29 baseline on food diaries, to facilitate weight loss. Guidelines fo r restriction ranged from 500 1000 kcals per day and followed the American Heart Association recomme ndations for macronutrient intake (i.e., 55% of energy coming from carbohydrates, 30% from fats, and 15% from protein) ( 143 ) In addition to dietary restriction, participants engaged in a multi component exercise program that incorporated both structured and home based physical activity. For the aerobic exercise component, participants were instructed to walk for 150 minutes per week. The exercise regimen was based on the protocols designed for the ADAPT Study ( 113 ) and LIFE Trial ( 144 ) which demonstrated the safety and efficacy of walking plus light resistance training as the exercise modality for use in older adults with mild to moderate impairments in mobility. Participants attended three community based, supervised exercise sessions per week. Safety d uring exercise was monitored by trained interventionists, certified in CPR, and potential adverse experiences and symptoms were observed and recorded. For example, blood pressure and heart rate were monitored before and after each supervised exercise sessi on. Participants were supervised by exercise physiologists and certified personal trainers. Sessions included aerobic activity (i.e., walking), lower body resistance strength training, and flexibility exercises. During each session, participants completed two 15 minute bouts of walking, separated by lower body resistance training exercises (i.e., wide leg squat, standing leg curl, seated knee extension, side hip raise, and toe stand). Participants aimed for two sets of 10 repetitions of each exercise with 6 0 second rest between sets. Resistance baring exercises were monitored and resistance was gradually increased using adjustable ankle weights as participants improved their fitness. Upon completion of

PAGE 30

30 these exercises, participants performed a series of fle xibility exercises. To help participants estimate the intensity level at which they were exercising, the Borg Perceived Exertion scale, a 15 point self assessment tool ranging from ea sy was used and participants were asked to walk at an i ntensity level equivalent to supervised sessions, and the remaining 60 minutes were to be done at home. Successful Aging Educational Group The successful aging educational c ontrol group met monthly for group based health education lectures on topics relevant to aging, but did not include lectures on weight loss, diet, or physical activity. Participants were to maintain their usual eating and physical activity patterns, and we re asked not to engage in any intentional weight loss for six months. As an incentive for participation in the control group, the full 24 week DR+E intervention was offered at the end of their six month assessment. Measurements Body weight. Body weight was measured in a fasting state following a morning void. Height and body weight were measured using a stadiometer, and body mass square of her height in meters. Central Adipo sity. Visceral and subcutaneous adipose tissue within the abdominal region was measured volumetrically over five contiguous axial slices (10 mm thickness) at the L4 L5 vertebral space by a T1 weighted 3D Magnetic Resonance Imaging (MRI) scanner (Philips Me dical Systems, Bothell, WA). The 3D data were collected using a fast gradient echo sequence, with TR=100ms, TE=10ms, flip angle of 3 selective fat suppression was utilized. The fat suppression

PAGE 31

31 enhanced muscle as high signal intensity pixels and adipose tissue as low signal intensity pixels to allow for segmentation. Images were analyzed using a freely available sof tware program called MIPAV (version 1.3; Medical Image Processing, Analysis and Visualization). Currently, multi slice volume MRI is empirically supported as the gold standard reference for measuring regional adipose tissue volumes ( 125, 145 ). Physical Fun ction. Two objective performance based tests were used to assess physical performance: The Short Physical Performance Battery and 400 m walk test. The Short Physical Performance Battery (SPPB) is related to mobility disability and activities of daily livin g disability ( 146 ) and is highly predictive of subsequent disability among non disabled older adults living in the community ( 147 148 ). Performance on the SPPB has been found to predict four year incident mobility disability ( 148 ). It is designed to assess lower extremity function by measuring three timed subtests: five timed repetitive chair stands, standing balance (i.e., hold tandem and semi tandem foot position for 10 seconds), and gait speed during a 4 m walk at from 0 to 4, with 0 indicating inability to complete test and 4 indicating maximal performance. A summary score from 0 to 12 is calculated for the SPPB, where higher scores indicate better physical function. The results of these three subtests are moderat ely correlated (Spearman correlation coefficients range from 0.39 to 0.48) based on over 5,000 participants in the Established Populations for Epidemiologic Studies of the Elderly ( 147 ). Interclass correlation coefficient values ranging from .88 and .92 h ave been reported for this measure ( 149 ) Regarding change in SPPB scores, based on data from the LIFE P trial, a minimally

PAGE 32

32 significant change is 0.3 to 0.8 points and a substantial change is 0.4 to 1.5 points ( 150 ). The 400 m walk test measures completion time (in seconds) on a standard adults between the ages of 70 to 79 years old, the 400 m walk t est has been shown to predict five year incident mobility disability ( 151 ). Participants were required to complete the 400 m course in 15 minutes, but were permitted to stop during the walk if necessary. Previous studies have demonstrated the high test retest reliability of the 400 m walk test ( 152 ). Regarding change in 400 m walk time, based on data from the LIFE P trial, a minimally significant change is 20 to 30 seconds and a substantial change is 50 to 60 seconds ( 150 ). Statistical Analyse s Statistical analyses were conducted using the Statistical Package for Social Sciences ( SPSS) for Windows, version 20.0 (IBM). Descriptive statistics were used to identify sample characteristics and provide summary indices of selected measures. One way analysis of co variance (ANCOVA) was used to determine any differences between the two grou and change outcomes The main outcomes of interest were change from baseline to six months for abdominal visceral adipose tissue and abdominal subcutaneous adipose tissue. Changes in MRI measures of central adiposity were compa red using ANC OVA, using age, race, and baseline measurements of VAT and SAT as the covariates, to evaluate differences between DR+E and successful aging groups. Values of VAT and SAT that are negative denote a decrease from baseline to six month follow up and values that are positive denote an increase from baseline. Correlation coefficients were used to quantify the relationships

PAGE 33

33 between changes in body composition, body weight, and physical performance outcomes with Person product moment correlation coeff icients.

PAGE 34

34 CHAPTER 3 RESULTS Sample Characteristics At baseline, participants (N = 25) had a mean age of 64.0 6.2 years weighed, on average, 94.8 15.7 kg and had a mean BMI of 36.1 5.1 kg/m 2 classifying them as obese (Class II) At baseline, t he re were no statistically significant differences between the DR+E group and successful aging control group on demographic, anthropometric, and physical function measures (see Table 1). Participants were evenly divided by race (African American and Caucasia n) across both groups ; however due to the relatively small sample size of each group, racial difference in body fat distribution were not analyzed Weight Loss and Physical Function The primary outcome of the parent study (i.e., walking speed) was found to increase significant among participants in the DR+E group compared to participants in the successful aging control group. Scores on the short physical performance battery also improved significantly among participants in the DR+E group and successful agin g control group, and participants in the DR+E group lost significantly more weight than participants in the control group. In accordance with the parent study, p articipants in the DR+E group had clinically meaningful improvements in walking speed and perfo rmed significantly better than participants in the control group (mean speed change SE in DR+E group = 0.17 0.04 m/s, p < 01 ; mean completion time change SE in control group = 0.03 0.04 m/s, p = 0. 66 ; mean difference = 0.15 m/s, p < .05 ) Scores o n the SPPB improved in both the DR+E and control groups (mean score change in the DR+E group SE = 1.8

PAGE 35

35 0.4, p < .01; mean score change in the control group SE = 1.0 0.4, p = 0.13) In addition, participants in the DR+E group lost significantly more weight than participants in the control group ( 7.02 1.1 kg vs. 0.16 1.1 kg p < 001). The average body weight loss for participants in the DR+E group was 7.3% of their initial body weight. Central Adiposity Both markers of abdominal adiposity (i.e ., visceral and subcutaneous adipose tissue) decreased significantly from baseline to six months among participants in the DR+E group. V isceral adipose tissue decreased significantly within the DR+E group compared to no significant change with in the succes sful aging control group ( mean VAT change in the DR+E group SE = 1057.4 347.0 cm 3 p < .01 ; mean VAT change in the control group SE = 199.9 378.2 cm 3 p = .87). As illustrated in Figure 1, t he DR+E group had a significantly greater reduction in vi sceral adipose tissue after six months compared to the contro l group ( mean VAT difference = 1257.4 521.2 cm 3 p < .05 ). Subcutaneous adipose tissue also decreased significantly within the treatment group after six months compared to no significant change with in the control group ( mean S AT change in the DR+E group SE = 2970.6 1345.1 cm 3 p < .05 ; mean S AT change in the control group SE = 1096.6 1406.9 cm 3 p = .66 ) As illustrated in Figure 2, participants in t he DR+E group had a significantly grea ter reduction in SAT after six months compared to participants in the contro l group (mean SAT difference = 4067.1 1979.8 cm 3 p = .05 ). Changes in Body Weight and Central Adiposity There was a large positive correlation between changes in body weight an d change in SAT (r = .57, p < .01). There was no significant correlation between change in

PAGE 36

36 body weight and ch anges in VAT (r = .30, p = .15) Notable, changes in VAT and SAT had a large, positive correlation (r = .725, p < .001). Changes in Central Adiposi ty and Physical Function Relationships between body composition changes and physical function were found to be non significant. T here was no correlation between t otal SPPB change scores and changes in VAT (r = .04 p = .84 ) or changes in SAT (r = .09 p = .69 ). In addition, there was no correlation between changes in 400 m walking speed (m /s ) and changes in VAT (r = .29 p = .17 ) or changes in SAT (r = .02 p = .93 ). Changes in Body Weight and Physical Function T here was no correlation between change in b ody weight and changes in 400 m walking speed (r = .30 p = .16 ) or total SPPB change scores (r = .32 p = .11 ).

PAGE 37

37 Table 3 1. Participant Demographics and Baseline Characteristics DR+E Mean (S.D.) Control Mean (S.D.) Age (years) 63.9 (4.8) 64.2 (7.6) Education (years) 14.2 (2.2) 15.1 (2.7) Race: Caucasian 7 6 African American 6 6 Weight (kg) 97.4 (12.9) 92.1 (18.5) BMI (kg/m 2 ) 36.9 (3.6) 3 5.1 (6.4) VAT (cm 3 ) 4532.6 (2028.5) 3920.6 (1982.1) SAT (cm 3 ) 15431.9 (4537.6) 14316.6 (5903.9) SPPB ( sc ore ) 9.2 (0.8) 9.3 (1.1 ) W400 (seconds) 434.9 (78.9) 388.9 (64.8) No significant group differences

PAGE 38

38 *Significant between group differences (p < .05) Figure 3 1 Mean c hanges in VAT volume between groups after 24 weeks of intervention. Visceral Adipose Tissue, cm 3

PAGE 39

39 Significant decrease from BL to W24 (p < .05) *Significant between group differences (p = .05) Figure 3 2 Mean changes in SAT volume between groups af ter 24 weeks of intervention Subcutaneous Adipose Tissue, cm 3

PAGE 40

40 CHAPTER 4 DISCUSSION Summary and Future Directions The purpose of the current study was to examine the impact of a six month, lifestyle base d weight loss intervention on changes in central body fat distribution in obese older women, a population that is at particularly high risk for obesity related comorbidities and subsequent disabilities. The major finding of this study was that a lifestyle based intervention produced significant changes in regional body fat distribution. Specifically, p articipants in the DR+E intervention group lost significant levels of visceral and subcutaneous adipose tissue located in the central region of the body. More over, participants in the DR+E intervention group had significant changes in body fat distribution after six months compared to the participants in the successful aging educational control group. The parent study ( 142 ) demonstrate d the feasibility, accept ability, and efficacy of this DR+E program in improving physical function in sedentary obese older adult women with mild to moderate physical impairments To determine the relationship between central adiposity and physical function, we examined changes i n body weight, regional body fat distribution, and physical function outcomes. Our hypotheses were that greater reductions in body weight would be associated with greater reductions in VAT and SAT, and g reater reductions in VAT and SAT would be associated with improve ments in physical performance In partial support of these hypotheses, reductions in body weight were found to be strongly and directly related to reductions in SAT. No relationship was found between changes in body weight and changes in VAT. A dditionally, no significant relationships were noted between changes in body weight or

PAGE 41

41 central adiposity with changes in physical function measures. Notably, there was a positive correlation between changes in VAT and SAT. To date only a few studies hav e investigated the effects of lifestyle interventions which combine dietary restriction and exercise on regional body fat distribution using technologies that can measure levels of internal visceral and subcutaneous adipose tissue. Although most studies sh ow a positive effect of weight loss on central adiposity, these results are limited by the use of anthropomorphic measures (i.e., waist to hip ration and waist circumference). While these measures are widely used as measures of adiposity and can be useful tool s in monitoring health status they are unable to assess body fat distribution directly. This makes them inaccurate for assessing health risks associated with central adiposity ( 153 ); therefore a strength of the present study compared to many previous studies was that central body fat depots (i.e., visceral and subcutaneous adipose tissue) were measured directly using magnetic resonance imaging technology. The findings of this study are promising regarding the potential of lifestyle based interventi ons to reduce chronic systemic inflammation by cytokines released in VAT and to decrease the risk for cardiometabolic diseases Even small differences in VAT volume can significantly improve plasma insulin and lipid metabolism, and alter the risk profile f or cardiovascular disease ( 51, 154 155 ). Similar results were reported by Santanasto and colleagues (2011) who found that physical activity plus weight loss significant ly reduced total abdominal fat, VAT, and SAT compared to no change in the successful agi ng control group (123) Moreover, m ean changes in VAT were significantly different between intervention groups. Notably, although SAT is not thought

PAGE 42

42 to be as harmful as VAT there is evidence of a critical VAT threshold effect ( 51 ). At the point where m eta bolic syndrome has begun to develop, SAT may become more like metabolically active VAT More evidence is needed to support this theory; however, our findings show that lifestyle based interventions can significantly reduce SAT, thereby minimizing potential deleterious effects of high levels of SAT. This study was designed to more closely examine the relationship between changes in body weight, body fat distribution, and physical function. In a previous study, d ecreases in abdominal visceral fat were dire ctly relat ed to change in body weight ( 117 ). We found a significant relationship between changes in body weight and in SAT, but this was not observed for changes in VAT. It has been previously shown that changes in regional body fat distribution can occur independently from changes in body weight and BMI ( 25 ) Therefore, changes in VAT may occur independently of weight loss and appear to be more strongly impacted by other components of the lifestyle based intervention (i.e., exercise training). While some studies have shown that VAT reductions can occur in the absence of significant weight loss among middle aged adults ( 156 158) this association has yet to be made in older adults. In contrast to hypotheses, n o significant relationships were observed betw een changes in central adiposity and changes in physical function measures Previous studies have demonstrated that intentional weight loss in obese older adults is associated with improvements in walking speed ( 135 ). Furthermore, data compiled from three randomized clinical trials combined showed that every 1 kg loss of fat mass predicts a 0.01 m/s increase in walking speed in obese older adults ( 135 ). Decreased flexibility, limited range of motion, and fatigue associated with both aging and central

PAGE 43

43 obesit y likely contribute to functional decline in obese older adults. In addition, a sedentary lifestyle may also contribute to a decline in functional status in this population. Changes in body fat distribution, particularly the accumulation of VAT are partic ularly hazardous in the pathogenesis of metabolic abnormalities (i.e., pro inflammatory state) that can contribute to both cognitive and functional decline. Thus, there are multiple contributors that can impact physical function and more research is needed to evaluate the relative effect of these factors. Findings from our study also showed no associations between changes in body weight and physical function. This finding is in contrast to findings from the literature, which suggest that there is a strong inverse relationship between weight change and physical function ( 108,113 ). In a recent study, the magnitude of weight loss was associated with improvements in walking speed, but not overall SPPB score ( 135 ). Still, it has also been suggested that the rel ationship between leg strength and gait speed is non linear in older adults ( 159 ). Additional research to assess the nature of this relationship will help to provide more information on the independent effects of weight loss and exercise. Strengths and L imitations The current study is significant and innovative as it is among the first randomized controlled trials to directly examine changes in body fat distribution in obese older women. We examined the response of abdominal adipose tissue to the recommen ded comprehensive lifestyle intervention for weight loss in obese older adults which was designed to minimize muscle and bone loss. Specifically, we measured v isceral and subcutaneous adipose tissue within the abdominal region directly by magnetic resonan ce i maging The design of our lifestyle based intervention wa s a comprehensive

PAGE 44

44 and safe approach for the treatment of obesity in older adults. Our ethnically/racially diverse sample of postmenopausal women is an important asp ect of this study because obes e older African American women represent an important underserved population for examining the effects of lifestyle approach es on central adiposity and physical functioning. The results of this study support the need for continued investigation on the key pathways linking central adiposity, inflammation and functional decline commonly observed in obese older adults. This study also has clinical importance as it highlights the importance of evaluating changes in abdominal fat distribution when working with obese older adults to monitor risk of cardiometabolic diseases, cognitive decline, and functional impairments. There are a few limitations to consider when interpreting the results of this study. First, the sample size was small as some women dropped out before the six month follow up visit and some image scans were unavailable or not interpretable. The small sample size did not allow for racial difference s to be examined within groups. Second, the duration of the study was only six months and therefore t he study duration may not have been sufficient to detect potential changes in body fat distribution, which may occur over longer time periods. Studies with larger study samples followed over longer time periods are needed to further evaluate the effects o f a comprehensive lifestyle intervention on central adiposity Third, t his experimental design did not allow for an understanding of whether dietary restriction alone, exercise alone, or the combined effects of dietary restriction plus exercise were respon sible for the observed reductions in VAT and SAT.

PAGE 45

45 Conclusions There is an urgent need for intervention studies testing lifestyle strategies f or improving or maintaining functional status in obese older adults. T he rising prevalence of obesity and its rela ted co morbid ities contribute to the increasing prevalence of disability among older populations particularly obese older African American women. As such, identification of older adults at high risk for disease and/or disability from increased central fa t depots will be an important focus of clinical practice and future research. The results of this study indicate that a comprehensive six month, lifestyle intervention consisting of dietary restriction plus a multi component exercise program can produce r eductions in abdominal body fat in obese older women with mild to moderate functional impairments. Specifically, dietary restriction plus exercise is an effective treatment approach for pro ducing significant reductions in visceral and subcutaneous adipose tissues in the high risk population of obese older African American and Caucasian women

PAGE 46

46 LIST OF REFERENCES 1. Vincent GK, Velkoff VA. The next four decades, the older population in the United States: 2010 to 2050. Current Population Reports 2010. Avail able at: http://www.census.gov/prod/2010pubs/p25 1138.pdf Accessibility verified April 14, 2013. 2. Flegal KM, Carroll MD, Ogden CL, Curtin LR. Prevalence and trends in obesity among US a dul ts 1999 2008 JAMA 2010; 3 : 235 241 3. Center for Disease Control and Prevention. Overweight and Obesity. Available at: http://www.cdc.gov/obesity/adult/causes/index.html Accessibility verified February 18, 2013. 4. National Heart, Lung, and Blood Institute, Nati onal Institutes of Health. The practical guide: identification, evaluation, and treatment of overweight and obesity in a dults. Available at: http://www.nhlbi.nih.gov/guidelines/obesity/prctgd_c.pdf Accessibility verified April 14, 2013. 5. Popkin BM, Duffy KJ. Does hunger and satiety drive eating anymore? Increasing eating occasions and decreasing time b etween eating occasions in the United States. Am J Clin Nutr 2010; 91:1342 7. 6. Center for Disease Control and Prevention. Physical Activity. How much physical activity do older adults need? Available at: http://www.cdc.gov/physicalactivity/everyone/guidelines/olderadults.html Accessibility verified February 18, 2013. 7. Katzmarzyk PT, Janssen I, Ardern CI. Physical inactivity, excess adiposity and premature morta lity. Obes Rev 2003; 4:257 29. 8. Mokdad AH, Marks JS, Stroup DF, Gerberding JL. Actual causes of de ath in the United S tates, 2000. JAMA 2004; 291(10):1238 1245. 9. National Heart, Lung, and Blood Institute, Nati onal Institutes of Health. Clinical guide lines on the identification, evaluation, and treatment of overweight and obesity in a dults Available at: http://www.nhlbi.nih.gov/guidelines/obesity/ob_gdlns.pdf Accessibility verif ied April 14, 2013. 10 Grotle M, Hagen KB, Natvig B, Dahl FA, Kvien TK. Obesity and osteoarthritis in knee, hip and/or hand: an epidemiological study in the general population with 10 years follow up. BMC Musculoskelet Disord 2008; 9:132. 11 Johnson WL Schur E, Noonan C, Ahumada S, Buchwald D, Afari, N. Chronic pain, overweight, and obesity: findings from a community based twin registry J Pain 2010; 1(7):628 635.

PAGE 47

47 12 Kushner RF, Foster GD. Obesity and quality of life. Nutrition 2000; 16(10):947 52. 13 Finkelstein, EA, Trogdon, JG, Cohen, JW, Dietz, W. Annual medical spending attributable to obesity: payer and service specific estimates. Health Affairs 2009; 28(5):w822 w831. 14 Ogden CL, Carroll MD, Kit BK, Flegal KM. Prevalence of obesity in the United States, 2009 2010. NCHS Data B rief 82. Hyattsville, MD: National Center for Health Statistics, 2012. 15. Pender JR, Pories WJ. Epidemiology of obesity in the United States. Gastroenterol Clin North Am 2005; 34(1):1 7. 16 Flegal KM, Carroll MD, O gden CL, Johnson CL. Prevalence and trends in obesity among US adults, 1999 2000. JAMA 2002; 288(14):1723 1727. 17. Villareal DT, Apovian C, Kushner R, Klein S. Obesity in older adults: technical Review and position statement of the American Society for Nutrition and NAASO, The Obesity Society. Am J Clin Nutr 2005; 82(5): 923 934. 18. Chapman, IM. Obesity paradox du ring aging. In: Mobbs, CV & Hof, PR, eds Body Composition and Aging Series: Interdisciplinary Topics in G erontology Basel (Switzerland): Karger; 2010: 20 37. 19. Heiat A, Vaccarino V, Krumholz HM. An evidence based assessment of federal guidelines for overweight and obesity as they apply to elderly persons. Arch Intern Med 2001; 161(9):1194. 20. Flegal KM, Graubard BI, Williamson DF, Ga il MH. Excess deaths associated with underweight, overweight, and obesity. JAMA 2005; 293(15):1861 1867. 21. Prentice AM, Jebb SA, Goldberg GR, et al. Effects of weight cycling on body composition. Am J Clin Nutr 1992; 56(suppl):209S 16S 22. Ferrucci L, Alley D. Obesity, disability, and mortality: a puzzling link. Arch Intern Med 2007; 167(8):750 1. 23. Hughes VA, Frontera WR, Roubenoff R, Evans WJ, Singh MA. Longitudinal changes in body composition in older men and women: role of body weight change and physical activity. Am J Clin Nutr 2002; 76 (2):473 481. 24. Hughes VA, Roubenoff R, Wood M, Frontera WR, Evans WJ, Singh MAF. Anthropometric assessment of 10 y changes in body composition in the elderly. Am J Clin Nutr 2004; 80(2):475 482.

PAGE 48

48 25. Zamboni M Mazzali G, Zoico E, et al. Health consequences of obesity in the elderly: A review of four unresolved questions. Int J Obes 2005; 29(9):1011 1029. 26. Gallagher D, Heymsfield SB, Heo M, Jebb SA, Murgatroyd PR, Sakamoto Y. Healthy percentage body fat ra nges: an approach for developing guidelines based on body mass index. Am J Clin Nutr 2000; 72(3):694 701. 27. Rosenberg I. Summary comments: epidemiological and methodological problems in determining nutritional status of older persons. Am J Clin Nutr 198 9; 50:1231 3. 28. Janssen I, Shepard DS, Katzmarzyk PT, Roubenoff R. The healthcare costs of sarcopenia in the United States. J Am Geriatr Soc 2004; 52 :80 85. 29. Rolland YM, Perry HM, Patrick P, Banks WA, Morley JE. Loss of appendicular muscle mass and loss of muscle strength in young postmenopausal women. J Gerontol A Biol Sci Med Sci 2007; 62(3):330 335. 30. Evans W J. Effects of exercise on body composition and functional capac ity of the elderly. J Gerontol 1995; 50A: 147 150. 31. Evans WJ, Campbell WW. Sarcopenia and age related changes in body composition and functional capacity. J Nutr 1993; 123(2)(suppl):465 8. 32. Delmonico MJ, Harris TB, Visser M, et al. Longitudinal study of muscle strength, quality, and adipose tissue infiltration. Am J Clin Nutr 2009; 90(6):1579 1585. 33. Villareal DT, Banks M, Siener C, Sinacore DR, Klein S. Physical frailty and body composition in obese elderly men and women. Obes Res 2004; 12(6):913 20. 34. Jarosz PA, Bellar A. Sarcopenic obesity: An emerging cause of f railty in older adults. Geriatr Nur 2009; 30(1):64 70. 35. Kyrou I, Tsigos C. Obesity in the elderly diabetic patient: is weight loss beneficial? No. Diabetes Care 2009; 32(2)(suppl):S403 S409. 36. Epel ES. Psychological and metabolic stress: A recipe fo r accelerated cellular aging? Hormones 2009; 8(1):7 22. 37. Estrada M, Kleppinger A, Judge JO, Walsh SJ, Kuchel GA. Functional impact of relative versus absolute sarcopenia in healthy older women. J Am Geriatr Soc 2007; 55(11):1712 1719. 38. Vincent HK, Vincent KR, Lamb KM. Obesity and mobility disability in the older adult. Obes Rev 2010; 11(8):568 79.

PAGE 49

49 39. Goodpaster BH, Carlson CL, Visser M, et al. Attenuation of skeletal muscle and strength in the elderly: the Health ABC Study. J Appl Physiol 2001; 90 (6):2157 2165. 40. Manini TM, Clark BC, Nalls MA, Goodpaster BH, Ploutz Snyder LL, Harris TB. Reduced physical activity increases intermuscular adipose tissue in healthy young adults. Am J Clin Nutr 2007; 85(2):377 384. 41. Visser M, Harris TB, Langlois J, et al. Body fat and skeletal muscle mass in relation to physical disability in very old men and women of the Framingham Heart Study. J Gerontol A Biol Sci Med Sci 1998; 53(3):214 221. 42. Koster A, Ding J, Stenholm S, et al. Does the amount of fat ma ss predict age related loss of lean mass, muscle strength, and muscle quality in older adults? J Gerontol A Biol Sci Med Sci 2011; 66(8):888 895. 43. Lebrun CE, van der Schouw YT, de Jong FH, Pols HA, Grobbee DE, Lamberts SW. Relations between body compos ition, functional and hormonal parameters and quality of life in healthy postmenopausal women. Maturitas 2006; 55(1):82 92. 44. Donohoe CL, Doyle SL, Reynolds JV. Visceral adiposity, insulin resistance and cancer risk. Diabetol Metab Syndr 2011; 3:12. 45 Berg AH, Scherer PE. Adipose tissue, inflammation, and cardiovascular disease. Circ Res 2005; 96(9):939 949. 46. Martha A, Christos S, Konstantinos M, Simon C, Theodoros D, Apostolos H. Age, weight and obesity. Maturitas 2012; 71 : 115 119. 47. DiStefa no PS, Curtis R, Geddes BJ. Insulin resistance, glycemic control and adiposity: key determinants of healthy lifespan. Curr Alzheimer Res 2007; 4(2):153 157. 48. El Atat FA, Stas SN, McFarlane SI, Sowers JR. The relationship between hyperinsulinemia, hyper tension and progressive renal disease. J Am Soc Nephrol 2004; 15(11):2816 2827. 49. Bouchard C, Bray GA, Hubbard VS. Basic and clinical aspects of regional fat distribution. Am J Clin Nutr 1990; 52:946 50. 50. Amati F, Pennant M, Azuma K, et al. Lower th igh subcutaneous and higher visceral abdominal adipose tissue content both contribute to insulin resistance. Obesity ( Silver Spring ) 2012; 20(5):1115 1117. 51. Freeland ES. Role of a critical visceral adipose tissue threshold (CVATT) in metabolic syndrome : implications for controlling dietary carbohydrates: A review. Nutr Metab (Lond) 2004; 1(1):12.

PAGE 50

50 52. Koh H, Hayashi T, Sato KK, et al. Visceral adiposity, not abdominal subcutaneous fat area, is associated with high blood pressure in Japanese men: The Oht ori Study. Hypertens Res 2011; 34(5):565 572. 53. Desprs J. Is visceral obesity the cause of the metabolic syndrome? Ann Med 2006; 38(1):52 63. 54. Cusi K. Nonalcoholic fatty liver disease in type 2 diabetes mellitus Curr Opin Endocrinol Diabetes Obe s 2009; 16:141 149. 55. Porter SA, Massaro JM, Hoffmann U, Vasan RS, O'Donnel CJ, Fox CS. Abdominal subcutaneous adipose tissue: a protective fat depot? Diabetes Care 2009; 32(6):1068 75. 56. Hansson, GK. Inflammation, atherosclerosis, and coronary arter y disease N Engl J Med 2005; 352 : 1685 1695. 57. Vogt, B, Fuhrnrohr, B, Muller, R., Sheriff, A CRP and the disposal of dying cells: consequences for systemic lupus erythematosus and rheumatoid arthritis Autoimmunity 2007; 40 : 295 298 58. De Visser, KE, Eichten, A., Coussens, LM. Paradoxical roles of the immune system during cancer development Nat Rev Cancer 2006; 6 : 24 37 59. Kissebah A, Murray A, Murray R, et al. Relationship of body fat distribution to glucose tolerance and clinical diabetes in obes e women. Clin Res 1980; 28:520A. 60. Kissebah AH, Vydelingum N, Murray R, Evans DJ, Kalkhoff RK, Adams PW. Relation of body fat distribution to metabolic complications of obesity. JClin Endocrinol Metab 1982; 54(2):254 260. 61. Svendsen OL, Hassager C, Christiansen C. Relationships and independence of body composition, sex hormones, fat distribution and other cardiovascular risk factors in overweight postmenopausal women. Int J Obes Relat Metab Disord 1993; 17(8):459 463. 62. Borkan GA, Hults DE, Gerzof SG, Robbins AH. Comparison of body composition in middle aged and elderly males using computed tomography. Am J Phys Anthropol 2005; 66(3):289 295. 63. Zamboni M, Armellini F, Harris T, et al. Effects of age on body fat distribution and cardiovascular risk factors in women. Am J Clin Nutr 1997; 66(1):111 115. 64. Sundquist J, Winkleby MA, Pudaric S. Cardiovascular disease risk factors among older black, Mexican American, and white women and men: An analysis of NHANES III, 1988 1994. J Am Geriatr Soc 2001; 49(2):109 116.

PAGE 51

51 65. Ford ES, Giles WH, Dietz WH. Prev alence of the metabolic syndrome among US adults. JAMA 2002; 287(3):356 359. 66. Keil J, Gazes P, Sutherland S, Rust P, Branch L, Tyroler H. Predictors of physical disability in elderly blacks and whites of the Charleston Heart study. J Clin Epidemiol 19 89; 42(6):521 529. 67. Ferrucci L, Harris TB, Guralnik JM, et al. Serum IL 6 level and the development of disability in older persons. J Am Geriatr Soc 1999; 47(6):639 46. 68. Cesari M, Penninx BWJH, Pahor M, et al. Inflammatory markers and physical per formance in older persons: the InCHIANTI study. J Gerontol A Biol Sci Med Sci 2004; 59A:M242 M248. 69. Visser M, Pahor M, Taaffe DR, et al. Relationship of interleukin 6 and tumor necrosis factor alpha with muscle mass and muscle strength in elderly men a nd women: the Health ABC study. J Gerontol A Biol Sci Med Sci 2002; 57A:M326 M332. 70. Ahluwalia N. Aging, nutrition and immune function. J Nutr Health Aging 2004; 8(1):2 6. 71. Wrnberg J, Gomez Martinez S, Romeo J, Daz LE, Marcos. A Nutrition, inflamm ation, and cognitive function. Ann N Y Acad Sci 2009; 1153:164 75. 72. Guralnik JM, Simonsick EM. Physical disability in older Americans. J Gerontol 1993; 48:3 10. 73. International classification of functioning, disability and h ealth. World Health Or ganization Wes site http://www.who.int/classifications/icf/en/ Accessed April 14, 2013. 74. Mor V, Wilcox V, Rakowski W, Hiris J. Functional transitions among the elderly: patterns, predictors, and related hospital use. Am J Public Health 1994; 84(8):12 74 80. 75. Branch LG, Jette AM. A prospective study of long term care institutionalization among the aged. Am J Public Health 1982; 72(12):1373 9. 76. Branch LG, Jette AM. The Framingham Disability Study: I. Social disability among the aging. Am J Public Health 1981; 71(11):1202 1210. 77. Guralnik JM, Simonsick EM, Ferrucci L, et al. A short physical performance battery assessing lower extremity function: association with self reported disability and prediction of mortality and nursing home admission. J Gerontol Med Sci 1994; 49:M85 M94.

PAGE 52

52 78. Manton KG. A longitudinal study of functional change and mortality in the United States. J Gerontol 1988; 43(5):S153 61. 79. Reuben DB, Siu AL. An objective measure of physical function of elderly outpatients: the P hysical Performance Test. J Am Geriatr Soc 1990; 38:1105 1112. 80. Peeters A, Bonneux L, Nusseider WJ, De Laet C, Barendreqt JJ. Adult obesity and the burden of disability throughout life. Obes Res 2004; 12(7):1145 1151. 81. Tabbarah M, Crimmins EM, Seem an TE. The relationship between cognitive and physical performance: MacArthur Studies of Successful Aging. J Gerontol A Biol Sci Med Sci 2002; 57 (4):M228 M235 82. cognitive def icit: the Framingham Heart Study. Neurobiol Agin g 2005; 26 :11 16. 83. Waldstein SR, Katzel LI. Interactive relations of central versus total obesity and blood pressure to cognitive function. Int J Obes (Lond) 2006; 30 :201 207. 84. Gunstad J, Paul RH, Coh en RA, Tate DF, Spitznagel MB, Gordon E. Elevated body mass index is associated with executive dysfunction in otherwise healthy adults. Compr Psychiatry 2007; 48 :57 61. 85. Coakley E, Kawachi I, Manson J, Speizer F, Willet W, Colditz G. Lower levels of ph ysical functio ning are associated with higher body weight among middle aged and older women. Int J Obes (Lond) 1998 ; 10 : 958 965. 86. Lang IA, Llewellyn DJ, Alexander K, Melzer D. Obesity, physical function, and mortality in older a dults. JAGS 2008; 56: 14 74 1478. 87. Rejeski WJ, Marsh AP, Chmelo E, Rejeski JJ. Obesity, intentional weight loss and physical disability in older adults. Obes Rev 2010; 11(9):671 685. 88 Alley DE, Chang VW, Doshi J. The shape of things to come: Obesity, aging, and disability. LDI Issue Brief 2008; 13(3):1 4. 89. Davison KK, Ford E, Cogswell M, Dietz W. Percentage of body fat and body mass index are associated with mobility limitations in people aged 70 and older from NHANES III. J Am Geriatr Soc 2002; 50 :1802 1809. 90. Banne rman E, Miller MD, Daniels LA, et al. Anthropometric indices predict physical function and mobility in older Australians: the Australian Longitudinal Study of Ageing. Public Health Nutr 2002; 5(5):655 662. 91. Guallar Castillon P, Sagardui Villamor J Ban egas J, et al. Waist circumferen ce as a predictor of disability among older adults. Obesity 2007; 15 : 233 244.

PAGE 53

53 92. Poirier P, Giles TD, Bray GA, et al. Obesity and cardiovascular disease: pathophysiology, evaluation, and effect of weight loss: an update o f the 1997 American Heart Association scientific statement on obesity and heart disease from the obesity c ommittee of the Council on Nutrition, Physical Activity, and Metabolism. Circulation. 2006; 113:898 918. 93. Despr s JP, Moorjani S, L upien PJ, Tremb lay A, Nadeau A, Bouchard C. Regional d istribution of body fat, plasma lipoproteins, and cardiovasc ular disease. Arteriosclerosis 1990; 10:497 511. 94. Despr s JP, Lemieux I, Be rgeron J, et al. Abdo minal obesity and the metabolic syndrome: contribution to global cardiometabolic risk. Arterioscler Thromb Vasc Biol 2008; 28:1039 49. 95. Despr s JP. Is visceral obe sity the cause of the metabolic syndrome? Ann Med 2006; 38:52 63 96. Fontana L, Eagon JC, Trujillo ME, Scherer PE, Klein S Visceral fat a dipokine secretion is associated with systemic inflammation in obese humans. Diabetes 2007 ; 56:1010 1013 97. Fujioka S, Matsuzawa Y, Tokunaga K, Tarui S. Contribution of intra abdominal fat accumulation to the impairment of glucose and lipid metabolism Metabolism 1987; 36: 54 59 98. Nakamura T Tokunaga K, Shimomura I, et al. Contribution of visceral fat accumulation to the development of coronary artery disease in non obese men Atherosclerosis 1994; 107 : 239 246 99. Desprs JP Nadeau A, Tremblay A, et al. Role of deep abdominal fat in the association between regional adipose tissue distribution and glucose tolerance in obese women Diabetes 1989; 38: 304 309 100. Nakajima T, Fujioka S, Tokunaga K, Matsuzawa Y, Tarui S. Correlation of intraabdominal fat accumulation and left ventricular performance in obesity. Am J Cardio l 1989; 64(5):369 373. 101 Center for Disease Control and Prevention. Injury prevention & c ontrol : data & s tatistics Ten leading causes of death and i nju ry. Available at: http://www.cdc.gov/injury/wisqars/LeadingCauses.html Accessibility verified February 18, 2013.

PAGE 54

54 102 Federal Interagency Forum on Aging Related Statistics. Older Americans 2008: Key indicators of well b eing Accessed at: http://ww w.agingstats.gov/agingstatsdotnet/Main_Site/Data/2008_Documents/tables/Ta bles.aspx Accessibility verified February 18, 2013. 103. Caplan B. Psychological assessment and practice in geriatric rehabilitation. In: Frank RG, Rosenthal, M, Caplan, B, eds. Ha ndbook of Rehabilitation Psychology 2 nd ed. Washington, DC: American Psychological Association; 2010: 95 119. 104. Arnold AM, Newman AB, Cushman M, Ding J, Kritchevsky S. Body weight dynamics and their association with physical function and mortality in older adults: The Cardiovascular Health Study. J Gerontol A Biol Sci Med Sci 2010; 65(1):63 70. 105. Knudtson MD, Klein BE, Klein R, Shankar, AA. Associations with weight loss and subsequent mortality risk. Ann Epidemiol 2005;15:483 491. 106. Reynolds MW Fredman L, Langenberg P, Magaziner J. Weight, weight change, mortality in a random sample of older community dwelling women. J Am Geriatr Soc 1999; 47(12):1409 14. 107. Miller SL, Wolfe RR. The danger of weight loss in the elderly J Nutr Health Aging 2008 ; 12(7):487 91. 108. Villareal DT, Banks M, Sinacore DR, Siener C, Klein S. Effects of weight loss and exercise on frailty in obese older adults. Arch Intern Med 2006; 166:860 866. 109. Jensen GL, Roy MA, Buchanan AE, Berg MB. Weight loss intervention for obese older women: improvements in performance and function. Obes Res 2004; 12( 11 ) : 1814 1820. 110. Pettee Gabriel K, Conroy MB, Schmid KK, et al. The impact of weight and fat mass loss and increased physical activity on physical function in overweight, postmenopausal women: Results from the WOMAN study. Menopause 2011; 18(7):759 765. 111. Shah K, Wingkun NJG, Lambert CP, Villareal DT. Weight lo ss therapy improves endurance capacity in obese older adults. JAGS 2008; 56:1157 1159. 112. Imayama I, Alfano CM, Kong A, et al Dietary weight loss and exercise interventions effects on quality of life in overweight/ obese postmenopausal women: a randomiz ed controlled trial. Int J Behav Nutr Phys Act 2011 ; 8:118. 113. Messier SP, Loeser RF, Miller GD, et al. Exercise and dietary weight loss in overweight and obese older adults with knee osteoarthritis: the Arthritis, Diet, and Activity Promotion Trial. Ar thritis Rheu 2004; 50(5):1501 1510

PAGE 55

55 114. The Diabetes Prevention Program Research Group. Reduction in the incidence of type 2 diabetes with lifestyle intervention or Metformin N Engl J Med 2002 ; 346:393 403 115 Villareal DT, Chode S, Parimi N, et al. Weight loss, exercise, or both and physical function in obese older adults. N Engl J Med 2011; 364(13):1218 1229. 116. Solomon TP, Sistrun SN, Krishnan RK, et al. Exercise and diet enhance fat oxidation and reduce insulin resistance in older obese adults J Appl Physiol 2008; 104(5):1313 9. 11 7 Nicklas BJ, Wang X, You T, et al. Effect of exercise intensity on abdominal fat loss during calorie restriction in overweight and obese postmenopausal wome n: A randomized, controlled trial. Am J Clin Nutr 2009; 8 9:1043 52. 118. Franz MJ, VanWormer J J, Crain L, et al. Weight loss outcomes: a systematic review and meta analysis of weight loss clinical trials with a minimum 1 year follow up. J Am Dietetic Association 2007; 107(10): 1755 1767 119. Carels RA, Darby L A, Cacciapaglia HM, Douglass OM. Reducing cardiovascular risk Factors in postmenopa usal women through a lifestyle change intervention. J Women s Health 2004; 13: 412 426. 120. NHLBI Obesity Education Initiative Expert s On the identification, evaluation, an d treatment of overweight and obesity in adults. clinical guidelines on the identification, evaluation, and treatment of ov erweight and obesity in adults T he evidence report. Obes Res 1998; 6(suppl 2):51S 209S. 121. Pi Sunyer X, Blackburn G, Brancati F, et al. reduction in weight and cardiovascular disease risk factors in individuals with type 2 diabetes. Diabetes Care. 2007; 30: 1374 1383. 122. Nicklas BJ Dennis KE Be rman DM Sorkin J Ryan AS Goldberg AP Lifestyle intervention of hypocaloric dieting and walking reduces abdominal obesity and improves coronary heart disease risk fact ors in obese, postmenopausal, African American and Caucasian women. J Gerontol A Biol Sci Med Sci 2003; 58(2): 181 9. 123. Santanasto AJ, Glynn NW, Newman MA, Taylor CA, Brooks MM, Goodpaster BH, Newman AB. Impact of weight loss on physical function with changes in strength, muscle mass, and muscle fat infiltration in overweight to moderately obese older adults: A randomized clinical trial. J Obesity 2011; 2011. http://www.hindawi.com/journals/jobes/2011/516576/ Accessed April 14, 2013. 124. Kay SJ Fiatarone Singh MA. The influence of physical a ctivity on abdominal fat: a systematic review of the literature. Obes Rev 2006; 7:183 200

PAGE 56

56 125. Ross R, Leger L, Morris D, de Guise J, Guardo R. Quantification of adipose tissue by MRI: relationship with anthropometric variables. J Appl Physiol 1992; 72:78 7 795. 126. Murphy JC McD aniel JL Mora K Villareal DT Fontana L Weiss EP Pre ferential reductions in intermuscular and visceral adipose tissue with exercise induced weight loss compared with calorie restriction. J A ppl Physiol 2012; 112(1):79 85. 127. Trussardi Fayh AP, Lopes AL, Fernandes PR, Reischak Oliveira A, Friedman R. Impact of weight loss with or without exercise on abdominal fat and insulin resistance in obese individuals: a randomised clinical trial. Br J Nutr 2013; 10:1 7. 128. Goodpaster B H Delany JP Otto AD et al. Effects of diet and physical activity interventions on weight loss and cardiometabolic risk factors in severely obese adults: a randomized trial. JAMA 2010; 304(16):1795 802. 129. Shen W, Punyanitya M, Chen J, et al. Visceral adipose tissue : relationships between single slice areas at different locations and obesity related health risks. Int J Obes (Lond) 2007; 31(5):763 9. 130. Mathu s Vliegen EM. Obesity and the elderly. J Clin Gastroenterol 2012; 46(7):533 544. 131. Irwin ML, Yasui Y, Ulrich CM, et al. Effect of exercise on total and intra abdominal body fat in postmenopausal women. JAMA 2003; 289(3):323 330. 132. Ryan AS, Pratley RE, Elahi D, Goldberg AP. Resistive training increases fat free mass and maintains RMR despite weight loss in postmenopausal women. J Appl Physiol 1995; 79: 818 23. 133. Ryan AS, Nicklas BJ, Berman DM, Dennis KE. Dietary restriction and walking reduce fat deposition in the midthigh in obese older women. Am J Clin Nutr 2000; 72(3):708 13. 134. Thompson PD, Crouse SF, Goodpaster B, Kelley D, Moyna N, Pescatello L. The acute versus the chronic response to exercise. Med Sci Sports Exerc 2001; 33(6 ) (suppl) :438 445. 135. Beavers KM, Miller ME, Rejeski WJ, Nicklas BJ, Krichevsky SB. Fat mass loss predicts gain in physical function with intentional weight loss in older adults. J Gerontol A Biol Sci Med Sci 2012; 68(1):80 6. 136. Bouchard DR, Dionne IJ, Brochu. Sarcopenic/obesity and physical capacity in older men and women: data from the Nutrition as a Determination of Successful Aging (NuAge) the Quebec longitudinal study. Obesity (Silver Spring) 2009; 17(11):2082 8.

PAGE 57

57 137. Buford TW, Anton SD, Judge AR. Models of accelerated sarcopenia: Critical pieces for solving the puzzle of age related muscle atrophy. Ageing Res Rev 2010; 9(4):369 383. 138. Chen H, Bermdez OI, Tucker KL. Waist circumference and weight change are associated with disability among elderly His panics. J Gerontol A Biol Sci Med Sci 2002 ; 57(1):M19 25. 139. Guallar Castilln P, Sagardui Villamor J, Banegas JR, et al. Waist circumference as a predictor of disability among older adults. Obesity (Silver Spring) 2007 ; 15(1):233 44. 140. Bonora E, Micc iolo R, Ghiatas AA, et al. Is it possible to derive a reliable estimate of human visceral and subcutaneous abdominal adipose tissue from simple anthropometric measurements? Metabolism 1995; 44:1617 1625. 141. Chumlea WC, Guo SS, Kuczmarski RJ, et al. Body composition estimates from NHANES III bioelectrical impedance data. Int J Obes Relat Metab Disord 2002; 26: 1596 1609. 142. Anton SD, Manini TM, Milsom VA, et al. Effects of a weight loss plus exercise program on physical function in overweight, older wom en: A randomized controlled trial. Clin Interv Aging 2011; 6:141 149. 143. Krauss R, Eckel R, Howard B, et al. AHA Diet ary Guidelines Revision 2000: A statement for healthcare professionals from the Nutrition Committee of the American Heart Association. Circulation 2000; 102: 2296 2311. 144. The LIFE Stu dy Investigators. Effects of a physical activity intervention on measures of physical p erformance: Results of the Lifestyle Interventions and Independence for Elders Pilot (LIFE P) Study. J Gerontol 2006 ; 61(11): 1157 1165 145. Heymsfield SB, Wang Z, Baumgartner RN, Ross R. Human body composition: a dvances in models and methods. Annu Rev Nutr 1997; 17:527 558 146. Guralnik JM, Ferrucci L, Pieper CF, et al Lower extremity function and subsequent disability: consistency across studies, predictive models, and value of gait speed alone compared with the short physical performance battery. J Gerontol A Biol Sci Med Sc i 2000 ; 55(4):M221 31. 147. Gural nik JM, Seeman TE, Tinetti ME, Nevitt MC, Berkman LF. Validation and use of performance measures of functioning in a non disabled older population: MacArthur studies of successful aging. Aging (Mil ano) 1994 ; 6(6):410 9.

PAGE 58

58 148. Guralnik JM, Ferrucci L, Simonsick EM, Salive ME, Wallace RB. Lower extremity function in persons over the age of 70 years as a predictor of subsequent disability. N Engl J Med 1995; 332(9):556 562. 149. Ostir GV, Volpato S, F ried LP, Chaves P, Guralnik JM. Reliability and sensitivity to change assessed for a summary measure of lower body function: Results from the J Clin Epidemiol 2002 ; 55 (9), 916 921. 150. Kwon S, Perera S, Pahor M, et al. Wha t is a meaningful change in physical performance? Findings from a clinical trial in older adults (The LIFE P Study). J Nutr Health Aging 2009; 13 (6) : 538 544. 151. Newman AB, Simonsick EM, Naydeck BL, et al. Association of long distance corridor walk perf ormance with mortality, cardiovascular disease, mobility limitation, and disability. JAMA 2006; 295(17):2018 2026. 152. Rolland YM, Cesari M, Miller ME, Penninx BW, Atkinson HH, Pahor M. Reliability of the 400 M u sual p ace walk test as an assessment of m obility limitation in older adults. J Am Geriatr Soc 2004; 52(6):972 976. 153. Molarius A, Seidell JC. Selection of anthropometric indicators for classification of abdominal fatness a critical review. Int J Obes Relat Metab Disord 1998; 22 : 719 727. 15 4. Fox CS, Massaro JM, Hoffmann U, et al. Abdominal visceral and subcutaneous adipose tissue compartments. Associations with metabolic risk factors in the Framingham Heart Study. Circulation 2007; 116: 39 48. 155. Goo dpaster BH, Kelley DE, Wing RR, Meier A, Thaete FL. Effects of weight loss on regional fat distribution and insulin sensitivity in obesity. Diabetes 1999; 48: 839 847. 156. Johnson NA Sachinwalla T Walton DW et al. Aerobic exercise training reduces hepa tic and visceral lipids in obese individuals without weight loss Hepatology 2009 ; 50 : 1105 1112 157. Slentz CA Duscha BD Johnson JL et al. Effects of the amount of exercise on body weight, body composition, and measures of central obesity: STRRIDE a r andomized controlled study Arch Intern Med 2004 ; 164 : 31 39 158. Ross R, Janssen I, Dawson J, et al. Exercise induced reduction in obesity and insulin resistance in women: a randomized controlled trial. Obes Res 2004; 12 : 789 98 159. Buchner DM, Larson E B, Wagner EH, Koepsell TD, de Lateur BJ. Evidence for a non linear relationship between leg strength and gait speed. Age Ageing 1996; 25 :386 391.

PAGE 59

59 BIOGRAPHICAL SKETCH Christy Karabetian obtained a Bachelor of Arts from Concordia University in Montreal, Que bec, with a double majo r in psychology and s ociology. She was involved in research at the Center for Research in Human Development at Concordia University, and in community servi ce work with underserved populations in Montreal. She completed a Master of Ar ts in m ental h ealth and b ehavioral m edicine from Boston University School of Medicine in Boston, Massachusetts. During her graduate studies, Ms. Karabetian received training in research and clinical skills at the Boston University Medical Center Following her studies, Ms. Karabetian accepted a position with a large, multi center NIA funded study, Comprehensive Assessment of Long term Effects of Reducing Energy Intake (CALERIE) and gained direct experience in clinical trial operations, behavioral counselin g, and collect data for outcome measures Ms. Karabetian participated in workshops for continuing education on such topics as sports nutrition, biofeedback, and motivational interviewing. In August 2011, she began her current position as a graduate researc h assistant in the Department of Clinical and Health Psychology at the University of Florida, under the mentorship of Dr. Stephen Anton. research interests are in the role that lifestyle factors have in influencing obesity, metabolic disea se conditions, and physical function.