Characterization of Chemosensory Proteins from the Red Imported Fire Ant, Solenopsis Invicta

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Material Information

Title:
Characterization of Chemosensory Proteins from the Red Imported Fire Ant, Solenopsis Invicta
Physical Description:
1 online resource (173 p.)
Language:
english
Creator:
Wanchoo, Arun
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Microbiology and Cell Science
Committee Chair:
KEYHANI,NEMATOLLAH
Committee Co-Chair:
KIMA,PETER EPEH
Committee Members:
KANG,BYUNG-HO
MAI,VOLKER
ACHE,BARRY W

Subjects

Subjects / Keywords:
chemosensory -- dichroism -- invicta -- ligand -- odorant -- solenopsis
Microbiology and Cell Science -- Dissertations, Academic -- UF
Genre:
Microbiology and Cell Science thesis, Ph.D.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Ants are one of the most successful animals on our planet, yet remarkably little is known about how they perceive chemical compounds, a process essential for their survival. Solenopsis invicta, the red imported fire ant employs a set of twenty one chemosensory proteins (CSPs) to aid in olfaction, chemical reception and or chemical transport. Our central hypotheses are that (a) CSPs display differential binding to a wide range of chemical and odorant molecules and (b) CSPs are differentially regulated in tissue and developmental specific patterns. Knowledge concerning the ligand specificity and gene expression patterns of CSPs can in turn can be used to make predictions regarding the role(s) of specific CSPs in chemoreception and other processes. Fourteen of the S. invicta chemosensory proteins (SiCSPs) were expressed and purified in recombinant form for the purposes of probing the activity and structural features of these proteins. Ligand binding profiles testing 78 different environmental and/or physiologically relevant compounds were determined for each protein. In addition, since CSPs are predicted to be predominantly alpha helical, circular dichroism polarimetry was used to test whether these proteins undergo any secondary structural conformational changes upon ligand binding. Real-Time quantitative polymerase chain reaction (RTQPCR) was used to examine the tissue and developmental gene expression profiles of all twenty-one CSPs. The ligand binding and gene expression profiles indicate complex patterns of chemical compounds perceived and tissue/developmental distribution. These data suggest that a subset of CSPs are multifunctional proteins with a subset involved in olfaction, while others potentially participate in chemical transport, release, and or sequestration.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Arun Wanchoo.
Thesis:
Thesis (Ph.D.)--University of Florida, 2014.
Local:
Adviser: KEYHANI,NEMATOLLAH.
Local:
Co-adviser: KIMA,PETER EPEH.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2016-05-31

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0045413:00001