The Non-Lantibiotic Mutacin Gene Nlmd and the Pyruvate Dehydrogenase Complex (Pdh) Operon

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Material Information

Title:
The Non-Lantibiotic Mutacin Gene Nlmd and the Pyruvate Dehydrogenase Complex (Pdh) Operon Models for the Regulation of Gene Expression in Streptococcus Mutans in Response to Aeration
Physical Description:
1 online resource (138 p.)
Language:
english
Creator:
Watts, Matthew O
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Medical Sciences, Immunology and Microbiology (IDP)
Committee Chair:
BURNE,ROBERT ARTHUR,JR
Committee Co-Chair:
BRADY,LINDA J
Committee Members:
JIN,SHOUGUANG
LORCA,GRACIELA L

Subjects

Subjects / Keywords:
bacteriocins -- caries -- dental -- mutans -- regulation -- streptococcus -- stress
Immunology and Microbiology (IDP) -- Dissertations, Academic -- UF
Genre:
Medical Sciences thesis, Ph.D.
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
Streptococcus mutans is a primary etiological agent of human dental caries.  The stress response pathways of S. mutans are important, as this is a virulent and opportunistic pathogen. Based on a previous microarray comparing the cultures of S. mutans grown under anaerobic or aerated conditions, the expression levels of several genes were altered under aerated conditions.  To determine the pathway(s) use be S. mutans to respond to aeration, three genes were chosen as model genes and the expression of each gene was assayed in various mutant strains lacking known regulatory proteins of S. mutans.  Each of the three genes is believed to be important for the virulence of S. mutans: non-lantibiotic mutacin D, a bacteriocin with antimicrobial effects on similar oral species; pyruvate dehydrogenase, a metabolic enzyme linked with acid tolerance; and glucosyltransferase B, linked with biofilm formation and sucrose-dependent attachment. Two-component systems and a global regulator played roles in the regulation of nlmD and the pdh expression.  CcpA was a dominant regulator of nlmD expression in S. mutans, while VicK and ComDE played roles in the expression of the mutacin in response to oxygen.  The expression of nlmD was also dependenton carbohydrate source.  However, CcpA controlled the expression of pdh,with VicK and ComDE playing a similar role in regulating the expression of pdh under certain environmental conditions.  The primary difference was that pdh expression was primarily controlled by carbohydrate catabolite repression, whereas nlmD was not even expressed in cells growing on a non-preferred carbohydrate source.  By defining the regulatory pathways that alter the transcriptome of S. mutans in response to oxygen, novel target pathways decrease the pathogenic potential of S. mutans maybe identified.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Statement of Responsibility:
by Matthew O Watts.
Thesis:
Thesis (Ph.D.)--University of Florida, 2014.
Local:
Adviser: BURNE,ROBERT ARTHUR,JR.
Local:
Co-adviser: BRADY,LINDA J.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2014-11-30

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2014
System ID:
UFE0045281:00001