Circadian Control of Adult Neural Progenitor Cell Behavior in Vivo and in Vitro

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Material Information

Title:
Circadian Control of Adult Neural Progenitor Cell Behavior in Vivo and in Vitro a Molecular and Genetic Screen of Regulatory Candidates for Neuroregenerative Therapies
Physical Description:
1 online resource (181 p.)
Language:
english
Creator:
Hoang-Minh, Lan B.
Publisher:
University of Florida
Place of Publication:
Gainesville, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Doctorate ( Ph.D.)
Degree Grantor:
University of Florida
Degree Disciplines:
Biomedical Engineering
Committee Chair:
Ormerod, Brandi K.
Committee Members:
Ogle, William
Carney, Paul R
Reynolds, Brent

Subjects

Subjects / Keywords:
cell -- circadian -- cycle -- estradiol -- genes -- hippocampus -- neurogenesis -- progenitors
Biomedical Engineering -- Dissertations, Academic -- UF
Genre:
Biomedical Engineering thesis, Ph.D.
Electronic Thesis or Dissertation
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )

Notes

Abstract:
Identifying the mechanisms that either stimulate or allowneurogenesis in the adult hippocampus could lead to the development of excitingnew strategies for treating neurodegenerative diseases and injuries. Chapter 1discusses the history, significance, and potential of endogenous andtransplantable stem cell strategies for CNS repair. Previous work suggests thatthe level of hippocampal neurogenesis is potentiated in nocturnal adult miceduring the dark (active) versus light (active) phase of the light-dark (LD) cycle.The experiments described in Chapter 2 directly tested whether neuralprogenitor cell (NPC) division, neuronal differentiation, and/or survival ofNPC progeny vary across the LD cycle. These experiments show that, during thedark cycle, more dividing NPCs are detected, and more surviving neurons areproduced in the hippocampal dentate gyrus than during the light cycle. Interestingly,more dividing cells were detected at night in the substantia nigra, but noevidence of neuronal differentiation among new cells was observed. Theexperiments in Chapter 3 show that the rate of NPC division in vivo is increased during the dark phase and the expression of cell cyclegenes in the hippocampus varies between light and dark phases. Chapter 3 experiments also show that moreprogenitor cells exit the multipotential state at night, and the expression levelsof genes stimulating neuronal differentiation and survival are significantly increasedin the hippocampi of mice harvested during the dark versus light phase,differentially between female and male adult mice. Next, in vitro experiments were conducted to test whether the NPC proliferationand/or daughter cell differentiation varied with diurnal-like changes ofpro-mitotic estradiol (which increases in mammals during the active versusinactive phase of the LD cycle; Chapter 4).In vitro analysis demonstrated thatNPC proliferation increases in the presence of a high versus low dose ofestradiol, while estradiol exposure has no effect on young neurondifferentiation or survival after 1 week in culture. Chapter 5 summarizes the circadiancycle-induced changes in components of neurogenesis that were identified inthis thesis and how they might influence normal hippocampal function, neuroregenerativestrategies, and potentially even strategies for combating brain tumors.
General Note:
In the series University of Florida Digital Collections.
General Note:
Includes vita.
Bibliography:
Includes bibliographical references.
Source of Description:
Description based on online resource; title from PDF title page.
Source of Description:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The University of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Thesis:
Thesis (Ph.D.)--University of Florida, 2012.
Local:
Adviser: Ormerod, Brandi K.
Electronic Access:
RESTRICTED TO UF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE UNTIL 2014-12-31
Statement of Responsibility:
by Lan B. Hoang-Minh.

Record Information

Source Institution:
UFRGP
Rights Management:
Applicable rights reserved.
Classification:
lcc - LD1780 2012
System ID:
UFE0045015:00001