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1 SEA TURTLE LIFE HISTORY PATTERNS REVEALED THROUGH STABLE ISOTOPE ANALYSES By KIMBERLY JEANNE REICH A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2009
2 2009 Kimberly Jeanne Reich
3 To my Mom
4 ACKNOWLEDGMENTS My dissertation was made possible by the endless support and encouragement of many wonderful people. I would first like to thank my advisor, Karen Bjorndal, for standing by me through the all of the ups and downs of the last years, onward and upward! I als o thank Alan Bolten for never failing to be there when I needed him. My committee members Karen Bjorndal, Alan Bolten, Lauren Chapman, Bruce MacFadden, Ray Carthy and Dave Hodell have been invaluable to my growth and development as a scientist I cannot thank them enough for their support and guidance. I am forever indebted to Carlos Martinez del Rio for thought provoking discussions and invaluable assistance with analysis of various components of my research. I am grateful to the many friends and colleagues who provided assistance with the development, implementation, and/or analysis of my work. I am particularly grateful to my undergraduate assistants, including Teresa Garcia, Joe Pfaller, Nick Osman, Sarah Lu ciano, Helene Jacobsen, Janine Sankar, Florence St. Pierre, Brandon Jarvis, Kristin Engelmann, and Jordan Taheri, all of whom contributed significantly to the success of my research. I am especially appreciative for the contributions of my many lab-mates, including Lindy Barrow, Sarah Bouchard, Peter Eliazar, Gabby Hrycyshyn, Kate Moran, Jeff Seminoff, Manjula Tiwari, Hannah Vander Zanden, and Brian Riewald (who is remembered fondly). I also thank the graduate students, post docs, and faculty in the Depart ment of Zoology for providing intellectual and emotional support throughout my graduate career. In particular, Iwould like to acknowledge Kelly Hyndman and Joanna Joyner. I am grateful to Dave Hodell and Jason Curtis for generously providing access to thei r labs and their assistance with stable isotope analyses. I would like to thank Blair Witherington, Chris Johnson and John Stiner for their assistance collecting samples.
5 I would like to give special thanks to Pete Ryschkewitsch, Mike Gunter, and Frank Dav is, all of whom went above and beyond in providing the support (and thousands of gallons of sea water) that allowed me to conduct a three year feeding trial with loggerhead turtles in the basement of Carr Hall. Finally, I owe an enormous debt to my family for the encouragement, love, and support they have provided throughout my time in graduate school. I do not know what I would have done without them. Conducting animal research requires the oversight of a number of permitting agencies, particularly when th is research entails working with endangered species. I am especially grateful to the Florida Fish and Wildlife Conservation Commission, the U.S. Fish and Wildlife Service, the National Marine Fisheries Service, U.S Department of the Interior National Par ks Service and the Institutional Animal Care and Use Committee at the University of Florida. This research was conducted under IACUC permits: D093, Z094, Z097, D242, Florida Fish and Wildlife Conservation CommissionMarine Turtle Permit # 016, and U.S. D epartment of the Interior National Park Service permit numbers CANA 2003SCI 0008; CANA 2004-SCI 003. Funding for my dissertation was provided by the Archie Carr Center for Sea Turtle Research, Disney Wildlife Conservation Fund, National Marine Fisheries S ervice, US Fish and Wildlife Service, And Florida Fish and Wildlife Conservation Commission Marine Turtle grants Program Canaveral National Seashore, The Knight Vision Foundation and Keir Kleinknecht. Numerous travel grants were provided by the University of Florida Graduate Student Council, the Department of Zoology at the University of Florida, the Comparative Nutrition Society, the Symposium on Sea Turtle Biology and Conservation, and the Society for Integrative and Comparative Biology.
6 TABLE OF CONTE NTS Page ACKNOWLEDGMENTS .................................................................................................................... 4 LIST OF TABLES ................................................................................................................................ 8 LIST OF FIGURES .............................................................................................................................. 9 ABSTRACT ........................................................................................................................................ 10 CHAPTER 1 INTRODUCTION ....................................................................................................................... 12 2 EFFECTS OF GROWTH AND TISSUE TYPE ON THE KINETICS OF 13C AND 15N INCORPORATION IN A RAPIDLY GROWING ECTOTHERM ................................ 19 Introduction ................................................................................................................................. 19 Methods ....................................................................................................................................... 21 Tissues .................................................................................................................................. 22 Trial 1: Hatchling Turtles .................................................................................................... 22 Trial 2: Juvenile Turtles ...................................................................................................... 23 Sample Preparation and Mass Spectrometry ..................................................................... 23 Statistical Analyses .............................................................................................................. 24 Results .......................................................................................................................................... 26 Trial 1: Hatchling Turtles .................................................................................................... 26 Trial 2: Juvenile Turtles ...................................................................................................... 27 Discussion .................................................................................................................................... 28 Contributions of Growth and Catabolic Turnover to the Rate of Isotopic Incorporation .................................................................................................................... 29 Assumption and Caveats in the Estimation of the Effect of Growth Rate on Isotopic Incorporation .................................................................................................................... 33 Differences in Isotopic Discrimination Among T issues and Between Age Classes ....... 33 3 THE LOST YEARS OF GREEN TURTLES: USING STABLE ISOTOPES TO STUDY CRYPTIC LIFESTAGES ............................................................................................ 45 Introduction ................................................................................................................................. 45 Methods ....................................................................................................................................... 46 Sample Collection ................................................................................................................ 47 Stable Isotope Analysis ....................................................................................................... 47 Results .......................................................................................................................................... 48 Discussion .................................................................................................................................... 48 4 BIMODAL FORAGING IN ADULT LOGGERHEADS ( CARETTA CARETTA ): CHANGES TO LIFE HISTORY MODELS ............................................................................. 55
7 Introduction ................................................................................................................................. 55 Methods ....................................................................................................................................... 57 Sample Collection ................................................................................................................ 57 Stable Isotope Analysis ....................................................................................................... 58 Statistical Analyses .............................................................................................................. 59 Results .......................................................................................................................................... 59 Discussion .................................................................................................................................... 60 5 CONCLUSIONS ......................................................................................................................... 74 Stable Isotopes an d Sea Turtle Ecology .................................................................................... 74 Advancing the Field ............................................................................................................. 74 Growth, Isotopic Discrimination, and Isotopic Incorporation in Loggerheads ............... 76 Solving a Mystery Lost Years of Small Green Turtles .............................................. 77 Loggerhead Life History A New Perspective ................................................................. 78 Future Research Needed ............................................................................................................. 78 Studies to Improve Our Ability to Use Stable Isotope Analyses in Sea Turtle Biology ............................................................................................................................. 78 Studies to Advance Our Knowledge of Sea Turtles and Our Ability to Conserve Them ................................................................................................................................. 79 LIST OF REFERENCES ................................................................................................................... 81 BIOGRAPHICAL S KETCH ............................................................................................................. 91
8 LIST OF TABLES Table page 2 1 In Trial 1, the isotopic incorporation of carbon from diet into tissues of hatchling 1313C(13C(0)13C(k st t............................................................................................................................ 41 2 2 In Trial 1, the isotopic incorporation of nitrogen from diet into tissues of hatchli ng k stt 42 2 3 In Trial 2, isotopic incorporation of carbon from diet into tissues of juvenile 1313C(13C(0) 13C(k st t............................................................................................................................ 43 2 4 In Trial 2, the incorporation of the nitrogen isotopic composition of diet into the tissues of juvenile loggerhead turtles was well described by the equation 1515N( 15N(0)15N( k st t .............................................................................. 44 4 1 Number of skin samples collected from nesting loggerheads each year by location: Canaveral National Seashore (CNS), Melbourne Beach (MEL), Juno Beach (JUN) and Pompano and Ft. Lauderdale beaches in Broward County (BRO). ............................. 72 4 2 Epibiont species identified on loggerheads nesting at Canaveral National Seashore, habitat where each epibiont species is typically found, and the number of turtles (oceanic or neritic ) on which the epibiont was identified. ................................................... 73
9 LIST OF FIGURES Figure page 2 1 Growth in a hatchling and b juvenile loggerhead turtles (Caretta caretta). Each line represents the growth trajectory of an individual. ................................................................ 37 2 2 1315N in loggerhead turtle hatchlings 0 203 days after a diet change .................................................................................................................................... 38 2 3 Correlations of fractional incorporations of carbon and nitrogen into skin, scute, red blood cells, plasma, and whole blood of loggerhead turtles in a Trial 1 b Trial 2. ............ 39 2 4 1315N in loggerhead turtle juveniles 0 232 days after a diet change. .................................................................................................................................... 40 3 1 Mean values ( 1 SD) of 13C and 15N () from oceanic -stage loggerheads ............... 51 3 2 Green turtle showing the 2 sampling sites anterior (A) and posterior (P). ......................... 53 3 3 1315N () from oceanic -stage loggerheads and neritic green turtles resident in seagrass habitat in the Bahamas ........................................................................................ 54 4 1 Locati ons of the four sampling sites. .................................................................................... 65 4 2 Distribution of stable isotope values from nesting loggerheads at four sites in Florida as d etermined by cluster analysis .......................................................................................... 66 4 3 P roportions of oceanic/pelagic foragers and neritic/benthic foragers among the four nesting locations (chi .................................... 67 4 4 Size distributions of oceanic foragers ( n = 158; diagonal hatching) and neritic foragers (n = 152; open bars) among nesting loggerheads in Florida ................................. 68 4 5 Clinal changes in haplotype frequencies and foraging strategies for female loggerheads nesting at four locations in Florida. ................................................................. 69 4 6 Life history pattern for loggerhead sea turtles showing the sequence of lifestages that pass through different marine zones ..................................................................................... 70
10 Abstract of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy SEA TURTLE LIFE HISTORY PATTERNS REVEALED THROUGH STABLE ISOTOPE ANALYSES By Kimberly Jeanne Reich August 2009 Chair: Karen A. Bjorndal Major: Zoology For my doctoral research, I used stable isotope analyses to explore aspects of sea turtle life history that had not been stud ied previously. I determined isotopic discrimination factors and the contribution of growth and catabolic turnover to the rate of 13C and 15N incorporation into skin, scute, whole blood, red blood cells, and plasma solutes in two age classes of rapidly growing loggerheads The isotopic discrimination factors of nitrogen ranged from 0.64 1.77 These values are lower than the commonly assumed 3.4 discrimination factors reported for whole body and muscle isotopic analysis. G rowth explained from 26 100% and 15 52% of the total rate of in corporation in hatchling and juvenile turtles, respectively. To my knowledge this is the first study to determine isotopic discrimination and incorporation in a reptile. I used stable isot opes of carbon and nitrogen retained in scute (the top keratin layer of a turtle s shell) to investigate the habitats, diets and duration of a missing life stage: the early juvenile stage of the green turtle, Chelonia mydas. I developed a technique to mi cro -sampl e successive layers (50) of keratin from small green turtles that had recently recruited to neritic waters. The oldest or outermost layer of scute contains the oldest retained isotopic history of diet and habitat available to scientists from a living turtle. Analyses revealed that small green
11 turtles spend 3 5 years as carnivores in oceanic habitats before undergoing a rapid shift to an herbivorous diet in neritic habitats. To investigate diet and habitat of Floridas nesting loggerhead populat ion prior to their recruitment to nesting grounds I collected skin at four locations in Florida in 2003 and 2004. Cluster analysis based on stable isotope signatures revealed a previously undocumented bimodal foraging pattern with females almost equally divided between oceanic/pela gic and neritic/benthic foraging. Oceanic foraging females were significantly smaller (mean CCLmin = 97.6cm) than neritic foraging females (mean CCLmin = 100.2cm) though there was considerable overlap bet ween the two groups The distribution of 35 species of epibionts collected from 52 loggerheads are consistent with the foraging habitats assigned to the turtles by cluster analysis.
12 CHAPTER 1 INTRODUCTION Spec ies with cryptic lifestages in unknown or inaccessible locations pose a special challenge to scientists and conservationists. Sea turtles undergo multiple ontogenetic shifts in habitat and foraging strategi es through several lifestages. Access to adult sea turtles is limited, due in part to the fact that, with the exception of brief periods when reproductive females return to the beach to deposit their eggs, adult tur tles spend their lives at sea. Upon hatching, neonate turtles leave the beach and disappear into the ocean. Despite intensive effort by scientists, sightings of hatchl ings of most species are rare. Juvenile turtles are also elusive, with few sightings reported prior to recruitment to know n juvenile foraging locations. In my dissertation, I demon strate how stable isotopes can be used to evaluate the ecology of unknown or inaccessible lifestages of sea turtles. Stable isotopes of carbon and nitrogen in the marine environment provide a tool to investigate habitat use as well as trophic level (Lathja and Michener 1994; Hobs on and Schell 1998). The use of stable isotope analysis of carbon and nitrogen to investigate movement, trophic level, and foraging habits of free ranging animals in the marine environment has increased steadily in the last decade, including studies of seabirds, marine mammals, and marine turtles (Best & Schell 1996; Cherel et al. 2000, Godley et al. 1998). A naturally occurring gradient exists for 13C values, in which 13C values are depleted in oceanic or pelagic habitats relativ e to neritic or benthic habitats (Lorian et al. 1992; Hob son et al. 1994; France 1995). 15N values with values increasing at higher trophic levels (Minagawa and Wada 1984; Macko et al. 1986). These naturally occurring gradients provide powerful tools for addressing questions of foraging dynamics in all lifestages and species of sea turtles.
13 Our understanding of the ecology of sea turtles depends in part on our ability to identify geographic regions used by animals for m ig rating, breeding, and feeding. In the field of ecology, stable isotope analyses are being used increasingly to investigate feeding habits, migratory patterns, and even geographic origins in migratory species that are difficult to study using conventional methods (Chamberlain e t al. 1997; Rooker et al. 2008; Wunder and Norris 2008). Conventional methods used to investigate dietary habits include direct observation, stomach content analyses, esophageal lavage, and f ecal analysis (Bjorndal 1997). These metho ds provide information on recent feeding events, but they cannot provid e a history of feeding habits. Similarly, satellite telemetry and mark recapture methods used commonly for tracking migratory species, including larger sea turtles, have provided inform ation on animal movement, but these techniques cannot provide any history of earlier movements (Addison et al. 2002; Godley et al. 2002; Hay e s et al. 2001; Hatase 2002b ; Polovina et al 2000; Seminoff et al. 2002). In addition, telemetry is often inappropr iate to study movements of small animals such as hatchling sea turtles because of the size of the instrumentation. Stable isotope studies can be especially useful to determine diets of animals that are difficult to observe in the wild (DeNiro and Epstein 1 978, Peterson and Fry 1987, Hobson 1999) and to investigate movement patterns of animals that are difficult to follow (Gannes et al 1998). Isotopic ratios can be useful to study diet, trophic interactions, and movements of a wide range of spec ies (Lajtha and Michener 1994). Through comparative studies of natural isotope abundance in diet and the isotope signatures in the tissues of the animal under investigation, one can begin to assemble a picture of where that animal has been and what it has consumed. In 1983, Killingley and Lutcavage used oxygen and carbon isotopes in barnacles removed from the shells of six large subadult loggerhead turtles (Caretta caretta ) to evaluate movements
14 of loggerheads between ne arshore and offshore habitats. Godley et al. ( 1998) successfully applied the stable isotope technique to predict known diets of loggerheads, green turtles (Chelonia mydas ), and leatherbacks ( Dermochelys coriacea ) from shallow water habitats in the Mediterranean, and Barrick et al. (1999) demonstrated that oxygen isotopes in bones of loggerhead and leatherback sea turtles could be used to identify the geographic regions t hat the turtles have occupied. Stable isotopes provide a powerful tool that can be used with minimally invasive sampling techniques to address questions of sea turtle migration and foraging dynamics. One factor hindering the interpretation of stable isotope data in studies of sea turtles has been the lack of data on the rate of isotopic incorporation and discrimination factors in sea tu rtles. The use of stable isotopes to investigate sea turtle foraging dynamics requires knowledge of the rate at which animals incorporate the carbon and nitrogen from their diets and the magnitude of the difference in isotopic composition between the anima ls diet and that of its tissues (discrimination factor) The mechanism of isotopic discrimination (change in isotope ratios in the animals tissues relative to the food source) and turnover rate (the time required for the existing isotopes in the tissue to be replaced) i s generally poorly understood. Isotopes present in the diet discriminate or are differentially assimilated into the tissue of the consumer and isotope ratios can vary among tissues within an ind ividual (Tieszen et al. 1983). Differences in diet tissue discrimination factors and isotopic incorporation rate may result from body size, age, diet, and met abolism (Tieszen et al. 1983) or growth rate (Chapter 2). Diet tissue discrimination factors and isotopic incorporation rate can be assessed by maintaining animals on a controlled diet of known isotopic value until isotopic turnover is achieved in all tissues of interest. In my dissertation, I have applied this technique to sea turtles and revealed novel information.
15 First, in Chapter 2, I conducted a 12 month diet study in which I investigated both the dynamics and consistency of carbon and nitrogen incorporation and discrimination in the tissu es of young loggerhead turtles after a diet shift. I conducted feeding trials on two age classes of loggerhead turtles. In the first trial, 108 loggerhead hatchlings were fed a pelleted diet that was significantly different in C and N values from the initial values of the tissues of inte rest (blood, skin, and scute). Over a period of 120 d ays, measurements of stable isotopes of carbon and nitrogen in blood, epidermis and scute were obtained at regular intervals. In trial two, eight juvenile loggerheads were switched to a diet with a different isotopic composition for an additional 232 days. I collected samples from the same tissues for stable is otope analyses in both trials. This is the first study in which both the isotopic incorporation and the isotopic discrimination factor in a variety of tissues is reported for a reptile. This study wa s essential to identify turn over and discrimination values. Results of this study demonstrate that 1) in both hatchling and juvenile turtles, growth contributes significantly to the rate of isotopic incorporation, and 2) this contri bution differed among ti ssues. In addition, isotopic discrimination values differ significantly among tissues in both hatchling and juvenile turtles, and 2) isotopic discrimination values of the same tissues from the two age classes (hatchling and juvenile) also show significant differences. These results suggest that discrimination factors may vary among diets and developmental stages. These data provide a baseline by which, for the first time, stable isotope analyses of sea turtle tissues can be interpreted using known values for diet tissue discrimination, and tissue turnover rates. Chapter 2 has been published. The citation is Reich K J K A Bjorndal and C del Rio (2008) Effects of growth and tissue type on the kinetics of 13C and 15N incorporation in a rapidly g rowing ectotherm. Oecologia 155:651663.
16 In Chapter 3, I employed stable isotope analysis to explore the "lost year" of green turtles. One of the greatest mysteries remaining in sea turtle conservation is how do endangered green turtles s pend their first years of life? Finding where hatchling and post hatchling turtles go and what they do during their lost years was identified by Carr (1952) as critical for the recovery of green turtle populations. The location of this lifestage remains a mystery for mos t species of sea turtles (Hughes 1974, Bolten et al. 1993, Bolten and Balazs 1995). It has been assumed that post hatchling green turtles are in oceanic habitats with carnivorous or omnivorous diets, but there are only a few anecdotal data available for th is age class (Bjorndal 1997). Oceanic sightings of small green turtles are rare and intensive efforts to locate post hatchling green turtles in the epipelagic environment of the open ocean have turned up relatively few clues as to where they are spending t he early years of their lives. I used 13C and 15N isotope analyses of scute-the top, keratin layer of a turtle shell -to investigate diet and trophic position of North Atlantic green turtles prior to their recruitment to neritic waters in Florida and the Bahamas. My studies confirmed that scute carries a record of previous diet and habitat use by comparing samples of old and new scute from green turtles that had recently recruited to seagrass meadows in the Bahamas. Isotope analyses of serial samples of s cute illustrate changes in stable nitrogen and carbon values with increasing depth from the scute surface. The oldest dietary record is retained in the outermost layer of scute and each successive layer (0.05mm) reveals more recent diet and habitat use. T o interpret the nitrogen and carbon values of these samples, I used discrimination values from a previous study of captive loggerheads (Chapter 2) and results of stable isotope analysis of juvenile loggerheads in developmental foraging grounds in oceanic habitats near the Azores
17 (Chapter 3). Analyses of 13C and 15N signatures of green turtle s cute provide evidence of a shift from a primarily carnivorous diet in the pelagic zone of oceanic habitats to an herbivorous diet in neritic habitats. A publication r esulted from the study reported in Chapter 3 The citation is Reich, K.J., K.A. Bjorndal, and A.B. Bolten (2007) The lost years of green turtles: using stable isotopes to study cryptic lifestages. Biology Letters 3:712714. In Chapter 4, I used stable is otope analysis of carbon and nitrogen from the skin of nesting loggerhead turtles to determine the foraging strategies of female loggerheads nesting in Florida. Florida has the largest nesting aggregation of loggerheads in the Atlantic and is one of only t wo populations worldwide with more than 10,000 loggerhead females nesting each year (Baldwin et al. 2003; Ehrhart et al. 2003) This population was assumed to have a totally neritic lifestyle, based on tag returns that are largely fishery dependent. Fisher y -dependent data can be misleading because tagged turtles will only be captured at fishing grounds rather than in all are as occupied by tagged turtles. As a result, the numbers of tag returns from neritic fishing grounds far outnumbered tag ret urns from ot her habitats ( National Marine Fisheries Service and U.S. Fish and Wildlife Service 1991). These results led scientists to conclude that adult loggerheads occupy neritic habitats. Using stable isotopes of carbon and nitrogen in the skin of nesting loggerheads on Florida nesting beaches allowed me to avoid the problem of fisherydependent data. I evaluated stable isotopes of carbon and nitrogen in samples of skin collected from 310 loggerheads nesting at four locations on the east coast of Florida. The stable isotope signature in skin represents a temporal integration of the isotopes assimilated during the synthesis of the tissue before the nesting season.
18 I also collected epibionts from 48 of the 310 loggerheads sampled for stable isotope a nalyses. Loggerheads serve as a substrate for a diverse array of epibionts (Caine 1986), and my hypothesis was that these epibiont communities would reflect the pre -nesting habitat of the host turtle. Analyses of stable isotopes and epibionts revealed that loggerheads nesting in Florida have a bimodal foraging strategy and are divided almost equally between oce anic and neritic foraging groups. Cha pter 4 has been submitted for publication. The citation will be: Reich, K.J., K.A. Bjorndal, M.G. Frick, B.E. Witherington C. Johnson, and A.B. Bolten. Bimodal foraging in adult loggerheads ( Caretta caretta ): changes to life history models. In my final chapter, I review how my studies have advanced the field of sea turtle biology and discuss where we should go from here.
19 CHAPTER 2 EFFECTS OF GROWTH AN D TISSUE TYPE ON THE KINETICS OF 13C AND 15N INCORPORATIO N IN A RAPIDLY GROWI NG ECTOTHERM I ntroduction The use of stable isotopes in animal ecology depends on the observation that, isotopically speaking, animals are what they eat plus or minus a small difference (called isotopic issues tissues of animals resemble the isotopic composition of their diets (DeNiro and Epstein 1978, 1981; Hobson and Clark 1992; Michener and Schell 1994), and (2) the match between the isotopic com position of an animals tissues and that of its diet is not perfect (Schoeller 1999). Both of these components are useful. The former allows us to determine the sources of the nutrients that animals assimilate, whereas the latter allows us to diagnose trop hic position (Peterson and Fry 1987; Post 2002). Using stable isotopes in animal ecology judiciously demands that we understand why there are often differences between the isotopic composition of an animal and that of its diet. The differences in the isotopic composition between an animals tissues and its diet can be due to three factors: (1) isotopic memory, (2) metabolic fractionation (defined as the difference in isotopic composition between reactants and products in biochemical reactions), and (3) is otopic routing (Martnez del Rio and Wolf 2005). The first of these factors is the best studied and the main focus of this study. The term isotopic memory refers to the observation that when animals change diets, the isotopic composition of their tissues d oes not change immediately to reflect that of their diet. Instead, tissues incorporate the diets isotopic composition with characteristic temporal dynamics (Fry and Arnold 1982; Phillips and Eldrige 2006). The dynamics of incorporation depend on a variety of factors including animal size (Carleton and Martnez del Rio 2005), nutrient composition of the diet (Gaye -Seisseggar et al. 2003, 2004), the catabolic turnover
20 of the tissue type (Tieszen et al. 1983; Hobson and Clark 1992; Martnez del Rio and Wolf 2005), and the animals growth rate (Fry and Arnold 1982; Hesslein et al. 1993; MacAvoy et al. 2001; Martnez del Rio and Wolf 2005). Although it is well established that the rate of isotopic incorporation into an animals tissues depends on both the rates of tissue growth and of catabolic turnover (Fry and Arnold 1982; Hesslein et al. 1993), only a handful of studies ha ve used stable isotopes to partition the contribution of growth and catabolic turnover to the rate of isotopic incorporation (reviewed by Ma cAvoy et al. 2001). These studies have revealed that in rapidly growing animals, net growth rate is an important determinant of the rate at which the isotopic signal of diet is incorpora ted into an animals tissues. I investigated both the dynamics and consistency of 13C and 15N incorporation into the tissues of two age classes of a rapidly growing ectotherm, the loggerhead sea turtle (Caretta caretta ), after a diet shift. Ectotherms such as sea turtles have relatively low protein turnover (Houlihan et al. 1995) and hence, presumably, low rates of tissue catabolic turnover (Hesslein et al. 1993; MacAvoy et al. 2001; Tominga et al. 2003). My research was guided by two hypotheses: (1) that growth would be the major factor determining the rate of isotopic incor poration, and (2) that the dominant effect of growth would erase the differences in isotope incorporation rates often observed among tissues (Fry and Arnold 1982; Hesslein et al. 1993; MacAvoy et al. 2001; Martnez del Rio and Wolf 2005). Although differen ces in isotopic incorporation among tissues have been relatively well documented in birds and mammals (Dalerum and Angerbjorn 2005), they have not been well studied in fish, amphibians, and reptiles. Differences in incorporation rates among tissues are use ful because they permit identifying dietary changes at contrasting time scales (reviewed by Dalerum and Angerbjorn 2005). Phillips and Eldrige (2006) have proposed that differences in isotopic
21 incorporation among tissues may allow constructing an isotopic clock to da te the time of a diet shift. My conjecture that rapid growth may homogenize the incorporation rates among tissues would limit the use of these two applications. By measuring body growth concurrently with the rate of isotopic incorporation of c arbon and nitrogen in multiple tissues I was able to (1) partition the contribution of growth and catabolic turnover to the rate of isotopic incorporation in several tissues, and (2) determine whether rate of isotopic incorporation varied among tissues. Me thods Loggerhead hatchlings from hatcheries in Broward County, Florida, were transported to the animal vivaria at the Department of Zoology, University of Florida (Gainesville, FL, USA) in June 2002. Hatchlings (n = 120; 20 hatchlings from each of 6 clutches) ranged in size from 4.3 to 4.9 cm in straight carapace length (SCL; mean SD = 4.6 0.11) and from 15.3 to 22.4 g in body mass (mean SD = 19.8 1.33). Turtles were marked for identification with 2 -mm plastic discs glued to the carapace and housed in indoor tanks at 26.5C (1) on a 12:12 light:dark cycle with 20W full spectrum fluorescent bulbs (vita -light) and 60 W outdoor flood lights. Each turtle was measured (SCL) and weighed every 10 days for the duration of the study. Hatchling and juvenile turtles were fed daily ( 3% of body mass). Food remaining after 45 min was removed from the tank. D iets for both phases of feeding trials were purchased in single batches from Mellick Aquafeed (Catawissa, PA) and stored at -samples (n = 9) w ere collected and throughout the study to test for temporal variation in the isotopic composition of experimental die ts. At the end of the trials, I released all turtles under Florida Wildlife Conservation Commission guidelines.
22 Tissues I analyzed the isotopic composition of whole blood, red blood cells, plasma sol utes, skin, and scute samples. I chose these tissues because they can be sampled non -invasively, and one of the goals of my study was to be able to release the turtles unharmed after its completion. In addition, I used blood and its components because they are widely used in stable isotope analyses in vertebrates (with the exception of fish) and are the tissues most widely used in isotopic incorporation studies (Dalerum and Ang erbjorn 2005). Approximately 0. 2 ml of blood was collected with a 25 gauge needle and syringe from the dorsal cervical sinus and transferred to a non -heparini zed container. A sub -sample (0. 1 ml) of whole blood was removed and the remaining blood (0 1 ml) was separated into plasma solutes, red blood cells, and white blood cells by centrifugation. After the tissues were separated white blood cells were discarded. Skin samples were collected from the dorsal surface of the neck region using a 2-mm steri le biopsy punch. Scute samples were collected from the newly grown, anterior edge of the second caudal s cute by scoring 6 mm2 with a #21 scalpel blade and peeling the scute from the carapace with forceps. Trial 1: Hatchling Turtles Hatchling turtles were fed for 203 days on a pelleted diet in which the main protein source 1315N SD of bulk diet equaled 0.26, and 3.25 0.47, respectively). This diet contained 3% lipids and 30% crude protein. Blood skin, and scute samples were collected from 108 loggerheads. Because small body size precluded repeated sampling of individuals, I grouped the turtles (2 hatchlings from each clutch pe r group) and sampled 1 of the 9 experimental groups (12 hatchlings) at each sampling period. Because hatchling turtles assimilate nutrients from the remaining yolk sac for a period of up to two weeks after leaving the egg (Kraemer and Bennett 1981), and due to the acclimation period
23 needed for hatchlings to begin feeding re g ularly on the prepared diet, I began my analysis of isotopic change eight days after the turtles were first offered food. Trial 2 : Juvenile T urtles One group of twelve hatchlings was maintained throughout the hatchling turtle trial under identical environm ental conditions but was not sampled. These turtles were the subjects of Trial 2. At the conclusion of the hatchling trial, the remaining turtles in this group (n = 8) were switched to a diet with an animal based protein source (40% protein and 12% lipid) and a 1315N SD of bulk diet equaled 0.29 and 9.45 0.37, respectively) for an additional 232 days. At the start of Trial 2, the eight juvenile turtles ranged from 9.0 to 13.1 cm SCL (mean SD = 10.6 1.35 cm) and from 105.0 to 385.7 g body mass (mean SD = 208.8 97.5 g). I collected the same tissues from juvenile and hatchling turtles, using the same protocols except that I sampled tissues of each juvenile turtle repeatedly. Sample Pr eparation and Mass S pectrometry Skin and scute samples were rinsed in distilled water, finely diced with a scalpel blade and dried to constant weight for 24 48 h at 60C. Blood samples (whole blood, plasma solutes, and red blood cells) were dried for 24 48 h at 60C and homogenized with a glass cell homogenizer. Lipids were extracted from dry skin and scute samples with petroleum ether in a Dionex Accelerated Solvent Extractor (ASE, Dodds et al. 2004). Lipid extraction was not performed on blood components tissue samples were loaded into pre -cleaned tin capsules, combusted in a COSTECH ECS 4010 elemental analyzer interfaced via a Finnigan -MAT ConFlow III device (Finnigan MAT, Brema n, Germany) to a Finnigan -MAT DeltaPlus XL (Breman, Germany) isotope ratio mass spectrometer in the light stable isotope lab at the University of Florida, Gainesville, FL, USA. Stable isotope
24 per thousand () relative to the standard as follows: = ([Rsample/Rstandard] 1) (1000) (2 1) where R sample and R standard are the corresponding ratios of heavy to light isotopes (13C/12C and 15N/14N) in the sample and standard, respectively. R standard for 13C was Vienna Pee Dee Belemnite (VPDB) limestone formation international standard. R standard for 15N was atmospheric N2. IAEA CH 13C = 15N = +0.4), cali brated monthly to VPDP and atmospheric N2, respectively, were inserted in all runs at regular intervals to calibrate the system and assess drift over time. The analytical precision of my measurements, measured as the SD of replicates of standards, was 0.111315N (N = 88 and 91, respectively). Statistical A nalyses I estimated growth rate using both a linear and an exponential model in 45 individual hatchlings (Trial 1) and 8 individual juveniles (Trial 2). I fitted the parameters of linear growt h with standard least squares procedures and estimated the fractional growth rate of the exponential model (k g in days linear fitting procedure (JMP). To assess whether hatchling and juvenile growth was better described by linear or by exponential models, I compared their coefficients of determination using paired t tests and the difference in Akaikes Information i AICmin, where AICmin is the smallest value in a comparison and AIC i is the value of the alternative model, Burnham and Anderson 2002). The comparison of r 2 and AIC gave the same r esults. Both models described my data equally well. Because ontogenetic growth in most animals is well described by sigmoidal functions with an exponen tial phase during the early stages of development (West et al. 2001;
25 Zimmerman et a l. 2001; Swingle et al. 2005) I chose the exponential over the linear growth model. I estimated the fractional rate of isotopic incorporation k st (in days l inear fitting procedure (JMP) using the equation d X(t)=d X(8) +(d X(0) d X(8))e kst t (2 2 ) incorporation of a tissue (OBrien et al. 2000; Martnez del were estimated by the same non linear procedure. Hesslein et al. (1993) demonstrated that for tissues growing exponentially k st equals the sum of net growth k gt of a tissue and catabolic turnover k dt (k st = k gt + k dt). If the tissues are at steady state, then growth equals catabolic degradation (k st = k dt). If the tissue is growing exponentially, then I can measure growth and partition the contribution of net growth and catabolic turnover to k st. The term k gt c an be measured as the mass specific rate of change in the size of the tissue (k gt), and k dt can be estimated by difference (k dt = k st t; see Hesslein et al. 1993). I assumed that the fractional rate of growth of tissues was the same as that of the whole hatchling (k g) and compared k st with k g using t tests. If k st estimated with Eq. 2 2 was significantly different from k g, I estimated k dt, the contribution of catabolic turnover to the rate of isotopic incorporation, as k st I estimated dition, for juvenile turtles, I 1315N) among tissues using univariate repeated measures A NOVA, after checking whether my data set satisfied sphericity assumptions, followed by Tukeys HSD. These analyses were not conducted for hatchling turtles (Trial 1) because their small size precluded repeated samples. To compare visually the
26 incorporation pattern that would result if accretion was the only process contributing to changes in the iso topic composition of tissues, I used k g instead of k st in Eq. 2 2 To assess the effect of variation in k g in the pattern of incorporation curves, I plotted isotopic incorporation curves using both the average value of k g and k g + SD and k g variation for k g in this exercise is justified because the distribution of k g values was close to normal [Shapiro Wilks W = 0.85 (N = 45) and 0.91 (N = 8), P > 0.2, for T rials 1 and 2, respectively]. Equation 2 2 assumes that the time that a C or N molecule stays in a tissue is distributed as a negative exponential with average residence time equal to 1/k st. I used average residence time, rather than the more widely used half life [Ln(2)/k st] because I could estimate a standard error for 1/k st as SE(1/k st) = SE(k st)/k st2, where SE(k st) is the asymptotic standard error o f k st (Stuart and Ord 1994). I estimated SE(k st) using the non linear procedure described above Results Trial 1: Hatchling Turtles Both linear and exponential models described the growth in mass of hatchlings relatively well (average r 2 SD = 0.97 0.02 and 0.98 0.02, respectively, Fig. 2 1 a). There was no significant difference between coefficients of determination of these models (paired = 0.91, P = 0.36, N = i i ranged from i ranged from 3.7 to 25.3), and in 5 i equaled 0 (these were the cases in which the coefficient of determination of the two models was identical). Because both models fitted the data set equally well, I chose the exponential model. The exponential model estimated a fractional growt h rate equal to 0.014g (SD = 0.002) day. Equation 2 2 1315N through time after a diet change adequately well in all tissues ( r 2 ranged from 0.88 to 0.95; Fig. 2 2 ). For carbon, only
27 plasma solutes and whole blood had ra tes of incorporation that differed significantly from the value expected from growth (Table 2 1 ). The estimated value of k dt = (k st k g) for these two tissues equaled 0.036 and 0.009 day, respectively. For nitrogen, the rate of isotopic incorporation into skin and red blood cells was indistinguishable from that expected by growth alone (Table 2 2 ). However, the incorporation into scute, plasma solutes, and whole blood was higher than that expected from growth. The value of k dt for these tissues equal ed 0.008, 0.040, and 0.014 day1315N were tightly and linearly correlated ( r = 0.99, P < 0.0006, Fig.23 1315N varied widely among 13C ranged from ble 2 1 15N ranged from (Table 2 2 ). Trial 2: Juvenile T urtles Both linear and exponential models described the growth in mass of hatchlings relatively well (average r 2 SD = 0.96 0.02 and 0.96 0.02, respectively, Fig. 2 1 b). In four i i ranged from 5.5 to 17.5) and in four i ranged from 4.3 to 26.3). Because both models des cribed the data equally well, I assumed that turtles grew exponentially with a fractional growth rate ( k g) equal to 0.012 0 .001g day). Equation 2 2 1315N after a diet change adequately ( r 2 ranged from 0.92 to 0.96, Fig. 2 4 ). The rate of fractional incorporation (mean SD = 0.027 0.010 day) and residence time (1/ k st, mean SD = 44.7 25.0 days) of carbon did not differ significantly among tissues [RM ANOVA, F 4,28 (tissue) = 1.02, P = 0.41 and F 4,24 = 0.37, P = 0.82, respectively, Fig. 2 4 Table 2 3 ]. The value of k st was significantly different from that estimated assuming that growth was the sole determinant of isotopic incorporation (i.e., k g = 0.012 day) in all tissues. Thus, replacement of carbon lost through catabolic turnover ( k dt = k st k g) contributed significantly to the rate of isotopic incorporation
28 (Table 2 3 ). The rate of catabolic turnover for carbon did not differ among tissues (mean SD = 0.015 0.011, RM ANOVA F 4,24 = 1.02, P = 0.41) and was significantly differen t from 0 in all tissues (one -sample t ranged from 3.16 to 4.67, P < 13C = 13Ctissue 13Cdiet) differed significantly among tissues (RM ANOVA F 4,24 = 48.40, P < 0.001, Table 2 3 ). All tissues had significantly positive isotopic discrimination relative to bulk diet (Table 2 3 ) except that of plasma solutes which was statistically indistinguishable from 0. The rate of nitrogen fractional incorporation and its residence time differed significantly among tissues [R M ANOVA, F 4,28 (tissues) = 7.0, P = 0.0007 and F 4,28 (tissues) = 10.39, P = 0.0001, respectively, Fig. 2 4 Table 2 4 ]. The value of k st was significantly different from that estimated assuming that growth was the sole determinant of isotopic incorporat ion (i.e., k g = 0.012) in all tissues. Thus replacement of nitrogen lost through catabolic turnover contributed significantly to the rate of isotopic incorporation. The rate of catabolic turnover ( k dt) of nitrogen also differed significantly among tissues [RM ANOVA, F 4,28 (tissues) = 7.10, P = 0.0006] and was significantly different from 0 in all tissues ( t ranged from 4.3 to 11.0, P < 0.01, Table 2 4 ). 15N) diff ered significantly among tissues [RM ANOVA, F 4,28 (tissues) = 85.82, P < 0.001] and was significantly positive only for skin and plasma solutes. 15N values that did not differ from 0, and scute tissue was significantly depleted in 15N relative to diet (Table 2 4 ). The rate of fractional incorporation of nitrogen was more variable among tissues than that of carbon ( F 5,5 = 65.34, P < 0.001, Fig. 2 3 ), and unlike in Trial 1, these rates were not correlated (mean r SD = 0.65, P = 0.78, N = 8). Discussion To my knowledge, this is the first study in which both the isotopic incorporation and the isotopic discrimination factor in a variety of tissues is reported for a reptile. Indeed, there is a
29 paucity of studies on the differences in isotopic incorporation and discrimination factors among tissues in ectothermic vertebrates. My results demonstrate that (1) in both hatchling and juvenile turtles growth contributes significantly to the rate of isot opic incorporation, and (2) this contribution differed amo ng tissues. In addition, (3) my results suggest that discrimination factors varied greatly among tissues, and perhaps among diets and/or developmental stages. Here I discuss each of these themes and consider their implications. My discussion is limited by the absence of comparable data sets on other ectotherms, and hence I framed some of the implications of my study as hypotheses to be tested rather than as conclusive patterns. Contributions of G row th and Catabolic Turnover to the Rate of Isotopic I ncorporation The rates of incorporation of dietary C and N differed among tissues in both hatchling and juvenile turtles, but the variation among tissues was considerably smaller than that found in other s tudies. In gerbils, half -lives of carbon in different tissues varied from 6.4 to 47.5 days (Tieszen et al. 1983); in Japanese quail, half -lives of carbon varied from 2.6 to 173.3 days (Hobson and Clark 1992). In juvenile turtles the half life, or median re sidence time, of carbon [estimated by multiplying the average residence times in Tables 2 1 2 2 2 3 2 4 by Ln(2) = 0.69] ranged from 27 to 35 days and that of nitrogen ranged from 11 to 31 days. Variation among tissues was slightly higher for hatchling turtles, but it still was lower than that found in previous studies (Tieszen et al. 1983; Hobson and Clark 1992). The median residence time of carbon in hatchlings ranged from 14 to 57 days and that of nitrogen ranged from 13 to 49 days. In agreement with other studies (summarized by Dalerum and Angerbjorn 2005), plasma solutes had relatively high incorporation rates of C and N in both trials. I hypothesize that the relative homogeneity in rates of isotopic incorporation among tissues is probably due to t he rapid growth that masked potential differences in catabolism among tissues. In hatchling turtles, several tissues had rates of incorporation that were indistinguishable from
30 whole body growth rate. In the tissues that differed, the contribution of growt h rate to incorporation ranged from 2 1 2 2 ). In juveniles, the contribution of growth rate to isotopic incorporation was high as well, and ranged from 31 to 46% for carbon, and from 15 to 52% for nitrogen. High contributions of growth to isotopic incorporation have been reported in several species of fish, tadpoles, and two species of snails (McIntyre and Flecker 2006). Indeed, as in my study, McIntyre and Flecker ( 2006) reported that incorporation rates were very similar to growth rates in catfish and tadpoles. The contribution of growth to the ra te of isotopic incorporation in the tissues of these ectotherms is high relative to that reported by MacAvoy et al. ( 2005) for adult mice in which growth accounted for only of the rate of incorporation of carbon and nitrogen. These observations could lead one to hypothesize that there is a difference in the relative contribution of growth and catabolic turnover to the rate of isotopic incorporation between endotherms (mice) and ectotherms (fish, amphibians, and reptiles). Although this hypothesis has merit, it must be qualified by differences in the developmental stages of the endotherms and ectotherms that have been investigated. The mice studied by MacAvoy et al. ( 2005) were close to their asymptotic, maximal size, whereas most of the studies on ecto therms have been conducted in rapidly growing animals. West et al. ( 2001) have hypothesized that the fraction of energy and nutrients used for growth, relative to other functions, is roughly the same for all species at the same stage of development, as mea sured relative to their asymptotic mass. Thus a newborn calf and a 6 -year old cod are at the same developmental stage (1/15th of their asymptotic mass) and should devote roughly the same fraction of their energy/nutrients to growth (Kohler 1964; West et al 2001). Following West et al. ( 2001), I hypothesize that the relative contribution of growth to isotopic incorporation will be roughly the same in ectotherms and endotherms, provided that the
31 animals are measured at comparable developmental stages (as def ined above). This hypothesis implies that, in general, growth rates will be more important determinants of isotopic incorporation in ectotherms than in endotherms. Among vertebrates, endotherms reach their asymptotic mass in a relatively short time and then stop growing (they are determinate growers), whereas many (albeit not all) ectotherms continue growing for most of their lives (they are indeterminate growers, Sebens 1987). The effect of growth on the rate of isotopic incorporation has several cons equences for the interpretation of isotopic measurements in the field. The first one was recognized by Perga and Gerdeaux ( 2005). These authors found that the isotopic composition of muscle in whitefish reflected the isotopic composition of prey consumed o nly in the spring and summer, when the somatic tissues of fish were growing. In contrast, the isotopic composition of liver, which had a higher contribution of catabolic turnover, tracked the isotopic composition of the diet closely throughout the year. Pe rga and Gerdeaux ( 2005) concluded that stable isotope analyses may be deceptive if the tissue measured reflects only the isotopic composition of food ingested during the time when the tissue is growing. Because many ectothermic vertebrates grow seasonally (Castanet 1994; Youngson et al. 2005), the confounding effects of seasonal growth on stable isotope analyses are probably a prevalent, albeit so far relatively unstudied, potentially confounding factor in stable isotope field studies. In seasonal environme nts, the isotopic composition of slow tissues, such as muscle may reflect the integration of dietary inputs over the growing season. Stable isotopes can provide an integrated view of animal diets (Araujo et al. 2007). However, the time window of integra tion depends on the rate at which animals incorporate the isotopic composition of their diets (Newsome et al. 2007). My study demonstrates that growth rate is an important determinant of isotopic incorporation rate, and thus of the time window of
32 integrati on of diets composition. Carleton and Martnez del Rio ( 2005) demonstrated an allometric relationship between the rate of isotopic incorporation and body size in full grown birds. Because growth rate is an allometric function of size (West et al. 2001), i t is likely that the window of isotopic integration of diets is size -dependent in animals with indeterminate growth. A second consequence of the effect of growth on the rate of isotopic incorporation is that growth can reduce the differences in the isot opic incorporation rates among tissues, and thus limit the usefulness of measuring the isotopic composition of different tissues to investigate diet at different time scales (Dalerum and Angerbjorn 2005). The homogenizing effect of growth may also reduce t he application of the isotopic clock proposed by Phillips and Eldrige ( 2006). Phillips and Eldrige ( 2006) demonstrated that confidence in the isotopic clock increases as the difference in incorporation rate s between tissues increases. My results suggest th at growth reduces the differences in isotopic incorporation among tissues, but it does not eliminate them. In both hatchling and juvenile loggerheads, plasma solutes had consistently high incorporation rates that, in all cases, were the result of a signifi cant contribution of catabolic turnover (Tables 2 1 2 2 2 3 2 4 ). Significantly the incorporation rate of plasma was higher, and thus the average residence time was shorter, than that of red blood cells. Plasma proteins are primarily synthesized in the liver (Turner and Hulme 1970; Adkins et al. 2002), a tissue with high rates of protein turnover and hence with high rates of isotopic incorporation (Haschemeyer and Smith 1979; Dalerum and Angerbjorn 2005). It is likely that liver and plasma proteins are in isotopic equilibrium (Tsudaka et al. 1971). The observation of a consistent difference in the rate of incorporation of blood cells and plasma proteins is significant because blood is one of the easiest tissues to sample non invasively in vertebrates and a single blood sample yields two tissues with different rates of isotopic incorporation.
33 Assumption and Caveats in the Estimation of the Effect of Growth R ate o n Isotopic I ncorporation My estimates of the relative contribution of growth rate and catabol ic turnover must be qualified by the assumptions that we re made. I used the approach of Hesslein et al. ( 1993) to partition the contributions of growth and catabolism to the rate of isotopic incorporation. Using this approach requires that the animals are growing exponentially (Hesslein et al. 1993) and that growth rates do not differ among tissues. In my study, turtle growth was very closely approximated by exponential functions (Fig. 2 1 ), and hence the first of Hesslein et al.s ( 1993) assumptions was satisfied. Unfortunately, I have no growth data for the tissues used in my study and cannot confirm the second assumption. However, tissue mass usually scales isometrically with body mass (Brown et al. 2000; Carleton and Martnez del Rio 2005) and hence th e fractional rate of tissue growth can probably be estimated by that of the whole body (Iverson 1984; Miller and Birchard 2005). Differences in Isotopic D iscrimi nation Among Tissues and Between Age C lasses The isotopic discrimination of nitrogen, defined 15N = 15Ntissues 15Ndiet when the animals tissues and diet are in equilibrium (Cerling and Harris 1999), is at the heart of the isotopic approach used to diagnose an animals trophic position. Most, albeit not all, studies that aim to diagnose an animals trophic position, use isotopic measurements of muscle or of the animals homogenized whole bodies (Post 2002; McCutchan et al. 2003; but see Bsl et al. 2006 and Wallace et al. 2006 as examples of studies using other tissues). However, one of the virtues of isotopic measurements is that they allow studying important aspects of an animals ecology noninvasively (Gustafson et al. 2007). My experiments allowed me to assess the variation in isotopic discrimination among tissues, and thus the feasibili ty of using tissues that can be collected non invasively in food web studies.
34 13C differed significantly among tissues between age classes. In hatchling turtles only skin and whole blood showed positive isotopic discrimination. In juvenile turtles, with the exception of plasma, all tissues showed small, though significant, positive isotopic discrimination. The carbon isotopic composition of plasma solutes was statistically indistinguishable from that of the diet. The isotopic di scrimination of tissues ranged from in plasma solutes to 1.77 in s cute, a difference of 2.15. My results are consistent with the values reported for isotopic discrimination of carbon (from 1993; Hobson et al. 1993; Pi nnegar and Poulin 1999; Roth and Hobson 2000; Lesage et al. 2002; Pearson et al. 2003; McCutchan et al. 2003; Seminoff et al. 2006), but several of the values reported here (1.77 and 2.62) are higher than the commonly accepted carbon discrimination of fro m 0 to 1 (DeNiro and Epstein 1978; Peterson and Fry 1987). 15N also differed significantly among tissues and between age 15N in hatchling tissues relative to that of the diet was positive for skin, scute, whole blood, and plasma solutes but negative for red blood cells. Isotopic discrimination of nitrogen ranged from 1.65 in skin to significantly from 0) in red blood cells. Juvenile turtle skin and plasma so 15N values that were significantly positive, red blood cells and whole blood had values that did not differ 15N relative to diet. Isotopic discrimination ranged from Wh 15N values? A mathematical model crafted by Martnez del Rio and Wolf ( 200515N decreases as the ratio of nitrogen incorporation in tissues exceeds the ratio of nitrogen loss. Because this ratio is highe r in growing young animals than in non growing adults, Martnez del Rio and Wolf ( 2005) predicted a lower
35 15N in growing anima ls than in nongrowing ones. My results support this prediction. In a meta analysis Vanderklift and Ponsard ( 2003) found signific 15N among the tissues of birds and mammals. The wide inter 15N in loggerhead turtles described here, 15N among tissues in loggerhead t urtles and those reported by Vanderklift and Ponsard ( 2003) have not been explained adequately, but have consequences for the interpretation of results of field studies that increasingly rely on tissues that can be sampled noninvasively (Sullivan et al. 2 006). 1513C, among tissues is variation in 1315N of individual amino acids can vary significantly (McClelland and Montoya 2002; Fogel and Tuross 2003), and thus diff erences in the amino acid composition of a tissue can lead to differences in isotopic discrimination among tissues (Howland et al. 2003). Pinnegar and Polunin ( 1999) postulated that amino acid profiles could influence the discrimination factor of di fferent tissues. However, to my knowledge, this effect has not been investigated systematically. The nitrogen isotopic composition of an animals tissues is widely used to diagnose trophic position in food webs (reviewed by Post 2002; Vanderklift and Ponsard 2003 ; McCutchan et al. 20031515N = 3.4 and the 2.3 values reported as the average discrimination factor for muscle and whole animal isotopic measurements by Post ( 2002) and McCutchan et al. ( 2003), respect ively (see also DeNiro and Epstein 1978; Peterson and Fry 1987; Kelly 1999). Seminoff et al. ( 20061315N values that differed greatly among several soft tissues (red blood cells, plasma solutes and skin) of a sea turtle ( Chelonia mydas ). The comparison between the values reported in this study and those reviewed by Post ( 2002), McCutchan et al. ( 2003), and Vanderklift and Ponsard ( 2003) must be
36 qualified by the observation that I did not use the tissues used in these reviews: musc le and whole animal homogenates. However, because the estimation of trophic level is sensitive to variation in 15N (Post 2002), studies that aim to estimate trophic position using stable isotopes may have to account for the type of tissue used (McCutchan et al. 2003). The increase d reliance of researchers on minimally invasive isotopic analyses demands that we begin understanding the variation in 15N among tissues.
37 Figure 2 1. Growth in a hatchling and b juvenile loggerhead turtles (Caretta caretta). Each line represents the growth trajectory of an individual. Growth trajectories are well described by exponential functions of the form y = ae(bt )
38 Figure 2 2 1315N in loggerhead turtle hatchlings 0 203 days after a diet change (see Trial 1, Eq. 2 2 ); curves fitted by a nonlinear routine (line). Expected 1315N if growth rate (k g = 0.014 day the rate of isotopic incorporation shown (dashed line) with k g 1 SD (dotted line).
39 Figure 2 3. Correlations of fractional incorporations of carbon and nitrogen into skin, scute, red blood cells, plasma, and whole blood of loggerhead turtles in a Trial 1 (r = 0.99, P < 0.0006) and b Trial 2 (NS), mean 1 SE. The diagonal line represents y = x
40 Figure 2 4. 1315N in loggerhead turtle juveniles 0 232 days after a diet change (see Trial 2, Eq. 2 2 ); curves fitted by a nonlinear routine (line). Expected 1315N if growth rate (k g = 0.012 day the rate of isotopic incorporation shown (dashed line) with k g 1 SD (dotted line)
41 Table 2 1. In Trial 1, the isotopic incorporation of carbon from diet into tissues of hatchling 1313C(13C(0)13C(k st t The value of kst was not significantly different from that estimated assuming that growth was the sole determinant of isotop ic incorporation (i.e., kg = 0.014) in skin, scute, and red blood cells (t -value; ns denotes not significant). However, plasma solutes and whole blood had higher rates of isotopic incorporation, than those expected from growth (** indicates p < 0.01). 13C = diet tissue discrimination (one -sample t test, and ** indicate significant difference from 0 with p < 0.05 and 0.01, respectively; ns = not significantly different from 0). Average residence time was estimated as 1/kst. Tissue Equation t value 13 C A verage residence time (days) Skin 20.08+10.65e 0.012(time) 1.2(ns) 2.62 0.34(**) 83.0 7.02 Scute 23.56+13.44e 0.016(time) 1(ns) 0.86 0.57(ns) 62.5 7.31 Red blood cells 23.34+13.44e 0.013(time) 0.5(ns) 0.64 0.73(ns) 76.9 11.34 Plasma solutes 22.41+8.00e 0.050(time) 18(**) 0.29 0.20(ns) 20.0 6.34 Whole blood 21.78+9.32e 0.023(time) 3.75(**) 0.92 0.34(*) 43.5 2.34
42 Table 2 2. In Trial 1, the isotopic incorporation of nitrogen from diet into tissues of hatchling 1515N( 15N(0)15N( k stt. The value of kst was not significantly different from that estimated assuming that growth was the sole determinant of isotopic incorporation (i.e., kg = 0.014) in skin and red blood cells (t -value, ns denotes not significant). However, scute, plasma solutes and whole blood had higher rates of isotopic incorporation than those expected from growth (** p < 0.01). 15N = diet tissue discrimination (one -sample t test, and ** indicate significant difference from 0 with p < 0.05 and 0.01, respectively; ns = not significantly different from 0). Average residence time was estimate d as 1/kst. Tissue Equation t value 15 N Average residence time (days) Skin 4.91+3.77e 0.015(time) 0.5 ns 1.65 0.12(**) 66.7 7.36 Scute 3.86+3.86e 0.022(time) 4.0 (**) 0.61 0.16(**) 45.5 5.48 Red blood cells 3.08+4.82e 0.014(time) 0 (ns) 0.25 0.30(ns) 71.4 10.66 Plasma solutes 3.57+2.86e 0.054(time) 20(**) 0.32 0.09(ns) 18.5 4.25 Whole blood 3.44+4.15e 0.028(time) 7(**) 0.19 0.08(*) 35.7 2.73
43 Table 2 3. In Trial 2, isotopic incorporation of carbon from diet into tissues of juvenile 1313C(13C(0)13C(k st t. The rate of fractional incorporation (kst) did not differ significantly among tissues (RM ANOVA; values in Equation column). kdt is rate of catabolic turnover of carbon; 13C = diet tissue discrimination; average residence time was estimated as 1/kst. and ** indicate when a one -sample t test revealed that kdt or 13C was significantly different from 0 with p < 0.05 and 0.01, resp ectively; ns = not significant. Means labeled by the same letter are not different from each other (RM ANOVAs). Tissue Equation kdt 13C Average residence time (days) Skin 20.18 1.54e 0.026(time) a 0.014 0.004(**) a 1.11 0.17(**) b 46.1 8.9 a Scute 19.512.56e 0.026(time) a 0.013 0.003(**) a 1.77 0.58(*) a 50.9 13.14 a Red blood cells 19.75 2.48e 0.027(time) a 0.014 0.003(**) a 1.53 0.17(**) ab 40.1 3.4 a Plasma solutes 21.661.09e 0.031(time) a 0.019 0.006(**) a 0.38 0.21(ns) c 39.6 9.1 a Whole blood 20.18 1.61e 0.026(time) a 0.014 0.004(**) a 1.11 0.18(**) b 46.1 8.9 a
44 Table 2 4. In Trial 2, the incorporation of the nitrogen isotopic composition of diet into the tissues of juvenile loggerhead turtles was well described by the equation 1515N( 15N(0)15N( k st t. The rate of fractional incorporation (kst) differed significantly among tissues (RM ANO VA; values in Equation column). kdt is rate of catabolic turnover of carbon; 15N = diet tissue discrimination; average residence time was estimated as 1/kst. and ** indicate when a one -sample t test revealed that kdt or 15N was significantly different from 0 with p < 0.05 and 0.01, respe ctively; ns = not significant. Means labeled by the same let ter are not different from each other (RM ANOVAs). Tissue Equation kdt 15N Average residence time (days) Skin 11.04 5.69e 0.023(time) b 0.011 0.001(**) b 1.60 0.07(**) a 44.9 3.1 a Scute 8.844.78e 0.076(time) a 0.064 0.015(**) a 0.64 0.09(**) c 16.2 2.3 c Red blood cells 9.51 6.57e 0.030(time) b 0.017 0.004(**) b 0.16 0.08(ns) b 36.3 3.4 ab Plasma solutes 10.96 7.24e 0.054(time) b 0.042 0.008(**) ab 1.50 0.17(**) a 22.5 5.1 bc Whole blood 9.59 6.06e 0.040(time) b 0.027 0.004(**) b 0.14 0.06(ns) b 27.7 3.5 bc
45 CHAPTER 3 THE LOST YEARS OF GREEN TURTLES: USING STABLE ISOTOPES TO S TUDY CRYPTIC LIFESTAGES Introduction Species with cryptic lifestages lifestages in unknown or inaccessible locations pose a special challenge to s cientists and conservationists. My study demonstrates how stable isotopes can be used to evaluate the ecology of an unknown or inacces sible lifestage of an organism. I use d stable isotopes to study the early juvenile stage of green turtles, Chelonia mydas a lifestage of unknown location. I solve d a 50 year mystery in the biology of marine turtle s posed by Archie Carr in 1952: where do green turtles s pend their first years o f life? After leaving the nesting beach as 5 cm hatchlings, green turtles disappear until they recruit to neritic habitats as > 20 cm juveniles and feed pri marily on seagrasses and algae. Archie Carr (1952) identified finding where hatchling and post h atchling turtles go and what do they do during their lost years as critical for the restoratio n of green turtle populations. In 1986, Carr postulated that the early juvenile stage of all sea turtle species was spent in the surface waters of oceanic hab itats (Carr 1986 ; 1987). Since that time, we have learned that North Atlantic loggerheads, Caretta caretta conform to Carrs hypothesis, and spend their first 10 years in oceanic habitats feeding primarily on sea jellies and salps (Bolten et al. 1998; Bjo rnd al et al. 2003; Bolten 2003a). Carrs hypothesis has been generally accepted as the working hypothesis for other sea turtle species (Musick and Limpus 1997). However, extensive searching in the North Atlantic have yielded thousands of sightings of logge rheads, but green turtles are rarely seen (Withe rington 2002; Bolten 2003a,b). Therefore, whether green turtles undergo an ontogenetic shift from oceanic to neritic habitats remains a question. Stable isotopes of nitrogen and carbon have been used to study migration, feeding ecology, and trophic structure in marine and terrestrial ecosystems (Hobson and Welch 1992; Post 2002;
46 Cerling et a l. 2006). Levels of 15N are used to determine trophic posit ion. In the marine environment, carbon isotopes can distinguish between oc eanic and neritic habitat use. Stable isotope values in keratinized tissues have been used to track changes in diet and habitat in baleen whales (Hobson and Schell 1998). I tested Ca rrs hypothesis with the stable isotope record stored in green turtle scute tissue the hard, keratinized tissue covering the boney shell of most chelonians. Scute is continually produced over the entire surface, so as a turtle grows and the boney shell i ncreases in area, scute accumulates and becomes thicker over the older areas, while areas of recent growth expansion are covered by only thin, young scute tissue. Once produced, scute is inert and, although it is susceptible to wear, retains a history of diet and habitat. I used stable isotope values from young loggerheads in oceanic habitats to evaluate the diets and habitats of lost year green turtles. If Carr is correct, the oldest scute removed from green turtles newly recruited to neritic foraging gr ounds should contain a stable isotope signature similar to that of the oceanic -stage loggerheads and the signature of the youngest tissue should approach that of resident green turtles in neritic habitats (Fig. 3 1a). Methods Scute samples were collected b etween 2001 and 2005 from two regions. At a long -term study site off Great Inagua, Bahamas (Bjorndal et al. 2005), samples were collected from 16 previously untagged green turtles and 2 previously untagged hawksbills < 36 cm s traight carapace length (SCL). These turtles (recruits) were assumed to have recruited to the study area in the previous year because a saturation mark recapture study has been conducted at this site for over 30 years Samples were also collected from 28 green turtles tagged in previous years and thus known to have been resident for at least 1 year (residents). In Florida, samples were collected opportunistically from 11 green turtles, 2 hawksbills, and 1 Kemps ridley (all < 36 cm SCL) that
47 st randed dead on the east coast. To minimize the possibility of stable isotope values being affected by body condition (Hobson et al. 1993), samples were only collected from turtles in apparent good health prior to death (e.g., turtles killed by boat strikes or drowning in fishing nets). Only isotope values from the oldest tissues were determined for Florida turtles; because turtle carcasses can float long distances before stranding, the habitat at time of death could not be determined. Sample Collection I used sterile biopsy punches, with 6 mm diameter t o remove scute samples encompassing the full depth of the scute from the surface (oldest scute) to the origin (newest scute). Samples were collected from the posterior and anterior sites of the second lateral scute ( F ig 2 2) Samples from the Bahamas were stored in 70% ethanol and samples from stranded turtles (previously frozen) were kept frozen until preparation for stable isotope analysis. Method of Collection of Scute Layers : Each scute sample was cleaned with isopropyl alcohol, rinsed in distilled water, and dried at 60C for at least 24 hr. Lipids were then removed from all samples using an Accelerated Solvent Extractor with p etroleum ether as the solvent. Posterior scute was ground to a depth of 50 m (yielding ~ 500 g) from the dors al side of each sa mple using a carbide end mill. I collected successive layers of scute by repeating this procedure on samples collected in 2005 from 8 green turtle recruits. The depth of each layer was dictated by the minimum quantity needed ( ~ 500 g) for analyses. Anterior scute samples were too thin to collect multiple layer sub -samples; anterior scute samples were h omogenized with a razor blade. Stable Isotope Analysis All samples were combusted in a COSTECH ECS 4010 elemental analyzer interfaced via a Finnigan MAT ConFlow III device (Finnigan MAT, Breman, Germany) to a Finnigan MAT DeltaPlus XL (Breman, Germany) isotope ratio mass spectrometer in the light stable isotope lab
48 at the University of Flor ida, Gainesville, Florida, USA. Stable isotope abundances were expressed in delta ( ) notation, defined as parts per thousand () relative to the standard as follows: = ([Rsample/Rstandard] 1) (1000) (3 1) where Rsample and Rstandard are the corresponding ratios of heavy to li ght isotopes (13C/12C and 15N/14N) in the sample and interna tional standard, respectively. Rstandard for 13C was Vienna Pee Dee Belemnite (VPDB) and for 15N was atmospheric N2. Internal standards were inserted in all runs at regular intervals to calibrate the syst em and assess drift over time. The analytical accuracy of measurements, measured as the SD of replicates of standards, was 0.11 for both 13C and 15N ( n = 88 and 91, respectively). Statistical analyses were performed with S Plus software (v. 7.03; Insightful Corporation). Results Signatures of carbon ( 13C) and nitrogen ( 15N) were significantly different (Wilcoxon rank sum tests, P < 0.0001, n = 16, 16) between the oldest and youngest scute tissues from green turtles that had recruited within the previous year to neritic seagrass habitats (recruits; n = 16; Fig. 2 1b). Isotope signatures were not significantly different between Azores loggerheads and the oldest tissues from green turtle recruits (Wilcoxon rank sum tests, n = 12, 16; P = 0.423 for 13C and P = 0.593 for 15N ). The youngest scute tissues from green turtle recruits and those of green turtles resident in the same neritic seagrass habitat for at least 1 year (residents), did not differ significantly in 15N values (Wilcoxon rank sum test, n = 16, 28; P = 0.150), but differed in 13C (P = 0.0003). Discussion My data sup port Archie Carrs hypothesis. Stable isotopes in scute tissue reveal that, before recruiting to neritic habitats, juvenile green turtles occupy similar habitats and f eed at the same
49 trophic level as do oceanic -stage loggerheads. As predicted, the isotope values of youngest scute tissue from recent recruits approach those of resident s on neritic foraging grounds. The 15N values are not significantly different between these two groups, but the 13C values are significantly lower in recruits, indicating that incorporation of the new nitrogen signature into scute tissue is m ore rapid than that of carbon. This pattern matches the relative rates of N and C incorporation into scute in captive juvenile loggerheads (Reich et al. 2008) and provides further support that N and C incorporations can be uncoupled and cannot be assumed to be equal (Hobson and Stirling 1997; Hobson and Bairlein 2003; Carleton and Mart nez del Rio 2005). A few data points in Fig. 3 1b do not c onform to the general pattern. The youngest scute point (square) in the midst of the oldest scute points (triangles) and the oldest scute point that falls within the youngest scute points probably represent, respectively, an individual that had just recruited and had not yet incorporated a neritic signature and an individual that had recruited earlier but had escaped tagging in the previous year. The two points for oldest tissue that fall between the two clusters represent sampling layers that combined tissues with the oceanic and neritic signatures, a transition habitat and diet between the oceanic and neritic signatures, or a different habitat and di et in the early life stage of these two individuals. Successive layers of scute store a chronologica l record of diets and habitats. I can draw conclusions about rates of change if rate of scute deposition is used as a proxy for time. These conclusions mus t be con sidered with caution because my 50-m sampling layers were based on the minimum amount o f sample needed for analysis; I do not know the biological significance of this depth. A relatively rapid and direct transition from oceanic to neritic habitats is indicated by the paucity of values between the primary oceanic and neritic signatures (Fig. 3 1c) and the oceanic signature still present in the youngest scute tissue of one turtle caught on neritic foraging
50 grounds (Fig. 3 1b). The oldest 2 to 3 layer s in most turtles had the same oceanic foraging signature (Fig. 3 1c) suggesting that these isotopic values represent either the entire, or a major portion of, the lifestage between hatching and recruitment to neritic habitats. The similarity of diets betw een oceanic -stage green turtles and loggerheads suggests that growth rates of young green turtles may be si milar to those of loggerheads. If so, we can estimate the duration of the oceanic stage of green turtles as the time required for loggerheads to grow to 2535 cm (sizes at which green turtles recruit to neritic habitats). Because oceanic -stage loggerheads in the eastern Atlantic reach 25 and 35 cm in approximately 2.8 and 4.6 yr, respect ively (Bjorndal et al. 2003), I estimate the duration of the green turtle oceanic stage is approximately 2.8 to 4.6 yr, as well. This range is similar to an estimate for green turtles based on skele tochronology of 3 to 6 yr (Zug and Glor 1999). Of course, variation in temperature, diet quality, and food availability woul d affect growth rates of green turtles. Preliminary scute samples from green turtles stranded dead in Florida (n = 11), hawksbills (Eretmochelys imbricata n = 4), and a Kemps ridley ( Lepidochelys kempi n = 1) indicate that all have a similar oceanic sig nature (Fig 3 3 ). Other populations of green turtles and, apparently, other species of sea turtles share similar oceanic habitats and diets in early juvenile stages. More extensive sampling is needed. Stable isotopes of scute provided insights into the ear ly juvenile stage of green turtles, a lifestage whose geogra phic location remains unknown. Tissues such as scute of marine turtles and baleen in whales that retain a stable isotope record provide a powerful tool for studying inaccessible lifestages.
51 Figure 3 1. Mean values ( 1 SD) of 13C and 15N () from oceanic -stage loggerheads (a) Mean values ( 1 SD) of 13C and 15N () from oceanic -stage loggerheads (n = 12) and neritic green turtles resident in seagrass habitat (n = 28). If Carrs conje cture is correct, these values should be equivalent to the shift in stable isotope values (indicated by arrow) from oldest to youngest scute tissues from green turtles recently recruited to neritic habitats. (b) Values of 13C and 15N () from 16 green turtle recruits, added to (a). (c) Values of 13C and 15N for successive scut e layers from 8 green turtles. Each line is an individual; each point is a different layer. -20 -18 -16 -14 -12 -10 -8 -6 -4 -1 0 1 2 3 4 5 6 7 815N (o/oo) a oceanic loggerheads neritic green turtles -20 -18 -16 -14 -12 -10 -8 -6 -4 -1 0 1 2 3 4 5 6 7 815N (o/oo) b oceanic loggerheads neritic green turtles recruit old tissue recruit new tissue
52 Figure 3 1 Continued. -20 -18 -16 -14 -12 -10 -8 -6 -413C (o/oo) -1 0 1 2 3 4 5 6 7 815N (o/oo) c
53 Fig ure 3 2. Green turtle showing the 2 sampling sites an terior (A) and posterior (P). Diagram illustrates the sequential sample layers from posterior scute samples. Grey tissue around the anterior and lateral sides of each scute is new tissue.
54 Figure 3 3 Mean values ( 1 SD) of 1315N () from oceanic -stage loggerheads (n = 12) and neritic green turtles resident in seagrass habitat (n = 28), individual values of 13C 15N () for the oldest scute tissue and youngest scute tissue from 16 green turtles that had recruited to seagrass habitat in the Bahamas within the previous year (recruits), and i 1315N () for the oldest scute tissue from small (< 36 cm) Florida green turtles (n = 11), hawksbills (n = 4) and Kemps ridley (n = 1). -22 -20 -18 -16 -14 -12 -10 -8 -6 -413C (o/oo) -1 0 1 2 3 4 5 6 7 8 9 1015N (o/oo) oceanic loggerheads neritic green turtles recruit new tissue recruit old tissue hawkbills Kemp's ridley Florida green turtles
55 CHAPTER 4 BIMODAL FORAGING IN ADULT LOGGERHEADS ( CARETTA CARETTA ): CHANGES TO LIFE HISTORY MODE LS Introduction Loggerhead hatchlings ( Caretta caretta ) emerge from nests on beaches along the southeastern US coast from Florida to North Carolina and enter the North Atl antic. They are incorporated into long -shore currents and are carried to oceanic foraging areas (Bolten 2003a) where they feed primarily o n sea jellies (Bjorndal 1997). Juvenile loggerheads recruit to neritic foraging areas at sizes between 46 to 64 cm car apace length, after about 7 to 12 years in oceanic habitats (Bjorndal et al. 2000, 2003). In neritic habitats, loggerheads shift to a diet primarily composed of hard-shelled, benthic invertebrates (Bjorndal 1997; Seney and Musick 2007). The original hypothesis was that this shift from the oceanic to the neritic environment is a unidirectional ontoge netic niche shift (Carr 1986). However, anecdotal reports (e.g., Eckert and Martins 1989) began to accumulate indicating that some individuals in neritic habitat s may return to oceanic habitats and some may never leave oceanic habitats except to reproduce. Thus, the life history model developed by Bolten (2003a) included these possibilities as speculative connections. Hatase et al. (2002a) were the first to confir m that some adult loggerheads utilize oceanic habitats between nesting seasons. Using stable isotopes (n = 149) and satellite telemetry (n = 5), they discovered nesting loggerheads from two different nesting beaches in Japan had been foraging in eit her oce anic or neritic waters. Through the use of satellite telemetry, Hawkes et al. (2006) documented the same foraging dichotomy for loggerheads (n = 10) from the population nest ing in the Cape Verde Islands. In both studies, females that foraged in oceanic habitats were significantly smaller than those foraging in neritic habitats, although there was overlap in body size i n Japan (Hatase et al. 2002a). In a study of large juvenile loggerheads captured in estuaries
56 of North Carolina, USA, satellite telemetry revealed that 10 loggerheads moved off the continental shelf and into oceanic habitats to forage, while 13 remained in neritic habitats (McClellan and Read 2007). Stable isotopes of carbon and nitrogen in the marine environment provide a tool to investigat e oceanic/pelagic vs coastal/benthic habitat use as well as trophic level (Lathja and Michener 1994; Hobson and Schell 1998). Studies have identified a naturally occurring gradient 1313C values tha n do coastal areas (Lorian et al. 1992; Hobson et al. 1994; France 1995). 15N values increasing at higher trophic levels (Minagawa and Wada 1984; Macko et al. 1986). The Atlantic USA nesting population of loggerheads is the largest loggerhead population in the Atlantic system. The loggerheads nesting in Florida are a large proportion of this population, but Floridas nesting population has declined dramatically by 49% between 1998 and 2006 (Witherington et al. 2009). Informat ion on the locations of foraging grounds for Florida loggerheads between reproductive seasons is incomplete but necessary to develop appr opriate management strategies. Loggerheads are listed as Endangered on the IUCN Red List and as Threatened under the U. S. Endangered Species Act. Do loggerheads nesting in Florida exhibit a polymorphism in foraging strategies? I used two approaches to answer this question and evaluate the foraging strategies of loggerheads before th ey arrived in Florida to nest. First, I evaluated stable isotopes of carbon and nitrogen in samples of skin collected from 310 loggerheads nesting at four locations on the east coast of Florida. The stable isotope signature in skin represents a temporal integration of the isotopes assimilated d uring the synthesis of the tissue before the nesting season. Second, I analyzed epibionts f rom 52 of the 310 loggerheads. Loggerheads serve as a substrate for a diverse array of
57 epibionts (Caine 1986), and these epibiont communities should reflect the pre -nesting habitat of the host turtle. Methods Sample C ollection S kin samples were collected from 310 loggerhead turtles nesting on beaches at Canaveral National Seashore (CNS), Melbourne Beach (MEL), and Juno Beach (JUN) during the first six weeks (2 May 15 June) of the 2003 and 2004 nesting seasons and, in 2003 only, from Pompano and Ft. Lauderdale beaches in Broward County (BRO) (Table 4 1; Fig. 4 1). Stable isotopes of carbon and nitrogen assimilated from the diet into the skin of juvenile loggerheads ha ve a mean residence time of 44.9 ( 3.1) d ays (Reich et al. 2008). Because residence times of isotopes in tissues decrease with increasing growth rates and because adult turtles grow more slowly than the juveniles used to calculate average residence time i n loggerhead skin, I a m confident that my sample period (45 days) is appropriate for assessing foraging location and trophic level of the turtles prior to their migration to the nesting grounds. I used a sterile 6 mm biopsy punch (designed for collecting e pidermis samples from humans) to collect samples of non keratinized skin from the shoulder area of each turtle after c leaning the area with alcohol. Skin samples were stored in 70 % ethanol at room temperature. Minimum curved carapace length (CCL) was mea sured from anterior notch to posterior notch; standard flipper tags were applied to both front flippers of untagged turtles to avoid re -sampling individuals. In 2003 and 2004, epibionts were collected from the carapace of 52 loggerhead turtles (also sample d for stable isotopes) nesting at Canav eral National Seashore. All epibionts present in an area of 20 cm2 on the posterior right quadrant of the carapace were collected and preserved
58 in 70% ethanol (Frick et a l. 1998; Pfaller et al. 2006). Samples were lat er sorted and identified to the lowest taxonomic level possible under light microscopy (magnification up to 1000x). Stable I sotope A nalysis Skin biopsy samples were rinsed in distilled water to remove any epibionts or other organic material and cleaned with isopropyl alcohol swabs. The surface epidermis was removed and hom ogenized with a scalpel blade. The homogenized sample was dried at 6 0C for a minimum of 24 hours. After drying, lipids were removed from all samples using an Accelerated Solvent Extra ctor (ASE) with petroleum ether as the solvent. Approximately 550 g of each dried, lipid -free sample was loaded into a pre -cleaned 4x6mm tin capsule. All samples were combusted in a COSTECH ECS 4010 elemental analyzer interfaced via a Finnigan MAT ConFlo w III device (Finnigan MAT, Breman, Germany) to a Finnigan MAT DeltaPlus XL (Breman, Germany) isotope ratio mass spectrometer in the light stable isotope lab at the University of Flori da, Gainesville, Florida, USA. Stable isotope abundances were expressed in delta ( ) notation, defined as parts per thousand () relative to the standard as follows: = ([Rsample/Rstandard] 1) (1000) (4 1) where Rsample and Rstandard are the corresponding ratios of heavy to light isotopes (13C/12C and 15N/14N) in the sample and international standard, respectively. Rstandard for 13C was Vienna Pee Dee Belemnite (VPDB) and for 15N was atmospheric N2. Internal standards were inserted in all runs at regular intervals to calibrate the system and assess drift over time. The analytical accuracy of my measurements, measured as the SD of replicates of standards, was 0. 09 for both 13C and 15N ( n = 88 and 91, respectively).
59 Statistical Analyses I 13C 15N signatures with cluster analysis (kmeans function, S -PL US v. 7.0.3; 1000 iterations). The kmeans function minimizes the within -cluster sum of squares from the cluster centers based on Euclidian distance (Crawley 2002). For each location for which I had 2003 and 2004 data (CNS, MEL, JUN), I tested for a year effect on the distribution of turtles between oceanic and neritic fora ging grounds using chi square. I also used chi -square tests to evaluate differences in the proportions of oceanic and neriti c turtles among the four nesting locations. To compare size differences, I compared CCL of the oceanic and neritic foraging groups with a t -test. I analyzed epibiont data to evaluate whether habitat -specific epibionts were consistent with my habitat (clust er) assignments. Epibiont species were characterized as either oceanic, neritic, or occurring in both habitats (Akoi 1997; Chace 1951; Foster et al. 2004; Frick et al 2003, 2004, 2006; McCain 1995; Williams 1984). I used chi -square tests to evaluate if the occurrences of neritic epibionts or oceanic epibionts were significantly different between the two f oraging groups of loggerheads. I conducted chi -square tests using the computer program CHIRXC (Zaykin and Pudovkin 1993), which calculates probabilities of independence using a Monte Carlo randomization method (1000 iterations). Statistical analyses were conducted in S -PLUS (v. 7.0.3) except for CHIRXC. Unless otherwise noted, alpha = 0.05. Results The 1315N signatures of the 310 nesting females fell into two clusters (Fig. 4 2). Cluster analysis assigned 158 females to oceanic foraging habitats and 152 females to neritic foraging habitats. The center f 13C = 14.9015N = 11. 213 and for the
60 13C = 15N = 7.488. Within -cluster sum of squares was 788.6 for oceanic and 567.3 for neritic. Comparing 2003 and 2004 distributions of turtles between oceanic and neritic foraging grounds within each nesti ng location, I found no significant difference for any nesting location (chi -s quare tests, df = 1, P >0.05). Therefore, for each nesting location, I combined data from the t wo years for CNS, MEL and JUN. The distributions between oceanic and neritic foragi ng grounds are significantly different among the four nesting locations (chi -square test, d 17.03, P = 0.0007). The proportion of oceanic foragers declines from north to south (Fig. 4 3). Oceanic -foraging females (CCL mean (SD) = 97.6 cm (6.0)) were significantly smaller than neritic -foraging females (CCL mean (SD) = 100.2 cm (5.7); t -test, df = 307, t = 3.903, P = 0.0001). There was, however, substantial overlap in CCL between the two groups (Fig. 4 4). The distributions of 35 species of epibionts (Table 4 2) on 33 oceanic loggerheads and 15 neritic loggerheads are consistent with the foraging habitats assigned by cluster analysis based on stable isotope signatures. The occurrence of neritic epibionts was significantly higher on neritic foraging females than on oceanic foraging females (chi -square, df = 1, The occurrence of oceanic epibionts was significantly higher on oceanic foraging females (chi Discussion Only two loggerhead ne sting aggregations in the world have more than 10,000 females nesting each year: Florida, USA, and Masirah, Oman (Baldwin et a l. 2003; Ehrhart et al. 2003). Analyses of stable isotopes and epibionts reveal that loggerheads nesting in Florida have a bimodal foraging strategy and are divided almost equally between oceani c and neritic foraging groups. Because of the large size of this loggerhead population and the large proportion of oceanic foragers, these results cause a major paradigm shift in the perceived roles of loggerheads
61 in marine ecosystems and in the appropriate management plans for the conservati on of this endangered species. Loggerheads are major marine predators (Bjorndal 2003), but we now know that, as large juveniles and adults, these predators are not supported entirely within neritic foodwebs, as pre viously supposed. Protecting large juvenile and adult loggerheads in oceanic habitats must now be added to the requirement of protecting them in neritic habitats, as has been the focus up to this t ime (National Marine Fisheries Service and U.S. Fish and Wildlife Service 1991). The differential distribution of oceanic and neritic epibionts on loggerheads with oceanic and neritic stable isotope signatures confirms the bimodal foraging pattern. My stu dy is the first to use epibionts to evaluate the bimodal for aging strategy in loggerheads. Earlier, Caine (1986) examined epibionts from loggerheads nesting at one site in South Ca rolina and 5 sites in Florida. He concluded that the epibionts revealed a separation between northern and southern loggerhead populations with the dividing point between Daytona Beach and Cape Canaveral, Florida. A significant trend exists for a decreasing proportion of nesting turtles to recruit from oceanic habitats from north to south alo ng the Florida coast (Fig. 3). Reasons for this north to south trend are not known, but may involve the proximity of the Gulf Stream current and the width of the continental shelf, both of which vary substantially among my sampling areas. Also the shorter distances between the extensive neritic habitats on the Great Bahamas Bank and my southernmost sampling areas may help account for the higher proportion of neritic -foraging turtles from my southern sampling areas. A study is underway to determ ine if this north to south trend continues northward for loggerheads nesting in Georgia, South Carolina, and North Carolina, and to integrate stable isotope results with tracks from satellite telemetry for loggerheads nesting in Georgia and North Carolina.
62 The mean size of turtles foraging in oceanic waters is significantly smaller than the mean size of turtles foraging in neritic waters (Fig. 4 4). This size difference is consistent with the findings of Hatase et al. (20 02a) and Hawkes et al. (2006). Agai n, I do not know the source of this difference. Oceanic turtles may be younger animals that may lat er change to neritic foraging. The relative food (either quantity or quality) and temperature regimes of the two habitats may result in slower growth rates i n oceanic turtles, although Hatase et al. (2004) found no relationship between growth rates and body size in loggerheads nesting in Japan, in a study in which they assumed small size would reflect an oceanic foraging strategy, based on their earlier study (Hatase et al. 2002a). However, they found a non-significant trend for smaller turtles to have longer intervals between nesting seasons, which they suggested may mean that loggerheads in oceanic habitats require more time to replenish nutrient stores neces sary for repr oduction due to a poorer diet. The occurrence of this bimodal foraging ha s not been evaluated in males. Two adult male loggerheads captured in neritic waters during the nonreproductive season in Japan were tracked by satellite telemetry (Sakamoto et al. 1998; Hatase et al. 2002b) into oceanic foraging grounds. Because both males were of relatively small siz e, Hatase et al. (2002b) suggested males may exhibit the same size related foraging dichotomy as in female loggerheads in Japan. Our understanding of the life cycle of loggerheads has been advanced substantially since last reviewed by Bolten (2003a,b). Fig 4 5a (modified from Bolten 2003a,b) shows the basic concept of a linear ontogenetic sequence of lifestages that pass through different marine zones with two speculative connections that wou ld disrupt the linear sequence. Bolten (2003a) suggested that juv enile neritic loggerheads m ay return to the oceanic zone. This suggestion has now been confirmed through satellite telemetry of neritic juvenile loggerheads moving into
63 oceanic habitats (McClellan and Read 2007) and is represented in Fig. 5b as the double -headed arrow marked as connection #2. Bolten (2003a) also suggested that some juvenile loggerheads may never enter the oceanic zone and thus complete their life cycle within neri tic habitats. This suggestion has yet to be confirmed and is represented by co nnection #5 in Fig. 4 5c. A major change in our conception of the life cycle of loggerheads is that some females that arrive at nesting beaches have come from oceanic foraging habitats and so me return to oceanic habitats. This new link, represented by con nection #1 in Fig. 4 5b, has bee n verified in both directions. The connection from oceanic to nesting beaches has been documented through stable isotopes (Hatase et al. 2002a; this stud y) and epibionts (this study). The connection from nesting beaches to t he oceanic zone has been documented through satellite telemetry (Hatase et al. 2002a; Hawkes et al. 2006). An additional question is whether adults in the oceanic zone derive from juveniles in the oceanic zone (speculative connection #3 in Fig. 4 5c). If s o, some loggerheads may have an entirely oceanic existence, other than the forays to the nesting beaches. Or do oceanic adults derive from neritic adults shifting habitats (speculative connection #4 in Fig. 4 5c). As more is learned about the movements of juvenile and adult loggerheads, we may find that loggerheads in all lifestages shift repeatedly between oceanic and neritic foraging habitats. My result of bimodal foraging strategies in female loggerheads nesting in Florida is consistent with the results for female loggerheads nesting in Japan (Hatase et al. 2002a) and Cape Verde Islands (Hawkes et al. 2006). Given the wide distribution of studies that have revealed bimodal foraging strategies in nesting loggerheads, it may well be that loggerheads throug hout the world exhibit this bimodal pattern. Of course, other populations must be studied; high priority should be given to the nesting populations in Brazil, Oman, Australia, and the
64 Mediterranean. Adult males in all of these breeding areas should also be sampled to determine if they exhibit the same bimodal strategy, and in simila r proportions, as the females. Where possible, results from stable isotope analyses, tracks from satellite telemetry, and epibionts should be integrated to provide the most robus t conclusions.
65 Figure 4 1. Locations of the four sampling sites. (1) Canaveral National Seashore (CNS); (2) Melbourne Beach (MEL); (3) Juno Beach (JUN); and (4) Broward County (BRO).
66 Figure 4 2 Distribution of stable isotope values from nesting loggerheads (n = 3 10) at four sites in Florida as determined by cluster analysis [O = oceanic forager ( n = 158), N = neritic forager (n = 152)]. Solid circles indicate the centers of the two clusters derived from the cluster analysis (see text). -18 -16-14 -12 -10 -8 -6 Carbon 0 5 10 15NitrogenO O O O O O O O O O O O O O O O O O O O O OO O O O O O O O O N N N N N N N N N N N N N O O O O O O O OO O O O O O O O N O N O N N N N N N N N N N N N N N N N N NN N N N N N N N N O O O O O O O O O O O O O O O O O O N O ON N N N N N N N N N N N N N N N N N N N O O O O O O O O O OO O O O O O O O O O O O O O O O O O O O O O O O O O N O N NN N N N N N N N N N N N N N N N N N N N O O O O O O O O O OO O O O N O O O O O N N N N N N N N N N N O O O O O O O O O OO N N N N N N N N N N N N N N N N N N N N N N N N N N N N NN O O O O O O O O O O O O O O O O O O O O N O O N N N N N NN N N N N N N N N N N N N N N N N
67 Figure 4 3. The proportions of oceanic/pelagic foragers (open bars) and neritic/benthic foragers (hatched bars) are significantly different among the four nesting locations (chi -square m north to south; see Fig. 4 1 for abbreviations. 0 20 40 60 80 100 Proportion by Foraging Area BRO JUN MEL CNS
68 Figure 4 4. Size distribution s of oceanic foragers ( n = 158; diagonal hatching) and neritic foragers (n = 152; open bars) among n esting loggerheads in Florida. Mean size of neritic foragers is signif icantly larger than the mean size of oceanic foragers (see text). 0 2 4 6 8 10 12 14 16 18 82 84 86 88 90 92 94 96 98 100 102 104 106 108 110 112 114 116 118 120 Number of TurtlesCurved Carapace Length (cm) Ocean ic
69 F igure 4 5. Clinal changes in haplotype frequencies and foraging strategies for female loggerheads nesting at four locations in Florida. Open triangles = haplotype CC -A1, closed triangles = haplotype CC -A2, open circles = oceanic/pelagic foragers, and closed circles = neritic/benthic foragers. These clines are independent (see text). For location abbreviations see Fig. 41. CNS MEL JUN BRO 0.0 0.2 0.4 0.6 0.8Proportion
70 Figure 4 6 Life history pattern for loggerhead sea turtles showing the sequence of lifestages that pass through different marine zones a) Basic Type 2 life history pattern for loggerhead sea turtles showing the sequence of lifestages that pass through different ma rine zones. Solid lines are confirmed connections; dashed lines are speculative connections (modified from Bolten 2003a, b, 2007; and Harrison and Bjorndal 2006).(b) Variations to the basic Type 2 life history pattern that have now been documented for lo ggerhead sea turtles. Connection (1) between oceanic and neritic juveniles is now known to be a 2 -way connection; McClellan and Read (2007) confirmed speculative connection of Bolten (2003a). Connection (2) is between the oceanic and internesting habitat s in the neritic zone for adult nesting females (Hatase et al. 2002a; Hawkes et al. 2006; this study). Connections (3), (4), and (5) are still speculative. Connection (3) indicates that oceanic juveniles may attain maturity in the oceanic zone; a portion of the loggerhead population may never inhabit neritic foraging grounds. Connection (4) indicates that adult loggerheads may move between the neritic and oceanic foraging zones. Connection (5) suggests that some juveniles may not have an early oceanic j uvenile stage and may complete their development in the neritic zone.
71 NERITIC ZONE Internesting Habitat OCEANIC ZONE Juvenile Stage Adult Stage Swim Frenzy Stage Post-Hatchling Trans. Stage Juvenile Stage Adult Stage Egg, Embryo, Hatchling TERRESTRIAL ZONE (a) NERITIC ZONE Internesting Habitat OCEANIC ZONE Juvenile Stage Adult Stage Swim Frenzy Stage Post-Hatchling Trans. Stage Juvenile Stage Adult Stage Egg, Embryo, Hatchling TERRESTRIAL ZONE (b) 1 4 3 2 5
72 Table 4 1. Number of skin samples collected from nesting loggerheads each year by location : Canaveral National Seashore (CNS), Melbourne Bea ch (MEL), Juno Beach (JUN ) and Pompano and Ft. Lauderdale beaches in Broward County (BRO). CNS MEL JUN BRO total 2003 44 60 41 47 192 2004 31 46 41 0 118 total 73 106 82 47 310
73 Table 4 2. Epibiont species characteristic of either oceanic/pelagic or neritic/benthic habitats identified on loggerheads nesting at Canaveral National Seashore, habitat where each epibiont species is typically found, and the number of turtles (oceanic or neritic) on which the epibiont was identified. Higher taxonomic designations are given in Pfaller et al. (2008). Typical habitat of epibiont Occurrence of epibiont Epibiont Oceanic / pelagic Neritic / benthic Oceanic/pelagic turtle (n=33) Neritic/benthic turtle (n=15) Lepas pectinata x 25 2 Membranipora tuberculata x 2 0 Anadara transversa x 0 2 Arbacia punctulata x 0 2 Bugula fulva x 0 4 Caprella equilibra x 0 4 Caprella penantis x 2 13 Caprella scaura x 2 2 Conopea galeata x 0 2 Leptogorgia virgulata x 1 6 Lytechinus variegatus x 0 1 Membranipora arborescens x 1 11 Mitrella lunata x 16 14 Molgula occidentalis x 2 14 Obelia dichotoma x 0 9 Ostrea equestris x 1 0 Podarke obscura x 0 3 Ricordia florida x 0 3 Strombus alatus x 0 2 Strombus gigas x 0 1 Thalamoporella floridana x 0 2
74 CHAPTER 5 CONCLUSIONS Stable Isotopes and Sea Turtle Ecology Advancing the Field My work has advanced both our ability to use stable isotopes to study sea turtles and our knowledge of sea turtle biology throu gh the use of stable isotopes. Before I began my doctoral studies, there was no information on isotopic discrimination or incorporation in sea turtles. As a result, studies in sea turtles were limited to using associate d organisms (Killingly and Lutca vage 1983) to predict sea turtle movements or dependent upon isotopic discrimination factors and incorporation rates generated by studies of other species (i.e. birds and terrestrial mammals) to predict the diet of sea turt le s (Godley et al. 1998, Hatase 2002a). The use of stable isotopes to investigate animal diets, habitat use, and trophic level requires understanding the rate at which animals incorporate the 13C and 15N from their diets (average residence time) and the factors that determine the magnitude of the difference (isotopic discrimination) in isotopic composition between the animals diet and that of its tissues. Results of my isotopic incorporation and discrimination study facilitate accurate interpretation of stable isotope data from multiple tissues of sea turtles, thus providing a tool for data analyses in future studies incorporating stable isotope analysis. Three factors can influence isotopic discrimination between an animals tissue and its diet: (1) iso topic memory, (2) metabolic fractionation (defined as the difference in isotopic composition between reactants and products in biochemical reactions), and (3) isotopic routing (Martnez del Rio and Wolf 2005). The fi rst of these factors, isotopic memory, is the primary focus of the research reported in Chapter 2 and refers to the finding that when animals change diets or habitats, the isotopic
75 signature of their tissue s do not immediately reflect the isotopic signat ure of their new diet and/or habitat. Fry and Arnold (1982) and Phillips and Eldrige (2006) established that tissues incorporate isotopic composition of new diet and/or change in habitat with cha racteristic temporal dynamics. The dynamics of incorporation depend on several factors including animal size (Carleton and Martnez del Rio 2005), nutrient composition of the diet (Gaye -Seisseggar et al. 2003; 2004), the catabolic turnover of the tissue type (Tieszen et al. 1983; Hobson and Clark 1992; Martnez del Rio and Wolf 2005), and the animals growth rate (Fry and Arnold 1982; Hesslein et al. 1993; MacAvoy et al. 2001; Martnez del Rio and Wolf 2005). Although it has been well established that the rate of isotopic incorporation into an animals tissues depends on both the rates of tissue growth and of catabolic turnover (Fry and Arnold 1982; Hesslein et al. 1993), there are few studies that use stable i sotopes to partition the contribution of growth and catabolic turnover to the rate of isotopic incorporation (rev iewed by MacAvoy et al. 2001). I measured both the dynamics and consistency of 13C and 15N incorporation into the tissues of two age classes of a rapidly growing ectotherm, the loggerhead sea turtle ( Caretta caretta ), after a diet shift. I report isotopic discrimination factors and incorporation rates (allowing me to predict the average residence time of C and N) of isotope 13C and 15N in whole blood, red blood cells, plasma, skin, and scute of both hatchling a nd juvenile loggerhead turtles. Knowing the isotopic discrimination factor of individual tissues allows for interpretation of carbon and nitrogen values in pre dator prey isotope analyses. Knowing the average time (average residence time) that a tissue retains the signature of the assimilated diet allows scientists to select the tissue that will most accurately assess the diet and habitat use for the period of interest, past or present. Ski n from juvenile loggerheads for example, has an average residence
76 time ( retains the isotopic signature of carbon and nitrogen from the turtles diet) o f 45 days (Chapter 2). T hus, skin collected from females early ( first 45 days) in the nesting season, is an appropriate tissue to identify the diet and habitat of female loggerheads prior to their arrival at the breeding and nesting grounds. Plasma, with an average residence time of days (Chapter 2) would be an appropriate tissue for looking at current diet or by analyzing a series of plasma samples from the same turtle over a period of months, identifying short term diet changes. Growth, Isotopic Discrimination and Isotopic Incorporation in Loggerheads In Cha pter 2 I present the contribution of growth and c atabolic turnover to the rate of 13C and 15N incorporation into several tissues that can be sampled non invasively (skin, scute, whole blood, red blood cells, and plasma solutes) in two age classes of a rapidly growing ectotherm loggerhead turtles I found significant differences in C and N incorporation rates and isotopic discrimination factors ( 13C = 13C tissues 13Cdiet and 15N tissues 15Ndiet) among tissues and between age classes. Growth explained from 26 to 100% of the total rate of incorporation in hatchling turtles and from 15 to 52% of the total rate of incorporation in juvenile turtles. Because growth contributed significantly to the rate of isotopic incorporation, variation in rates among tissues was lower than reported in previo us studies. The contribution of growth can homogenize the rate of isotopic incorporation and limit the application of stable isotopes to identify dietary changes at contrasting time scales and to determine the timing of diet shifts. The isotopic discrimina tion factor of nitrogen ranged from 0.64 to 1.77 in the turtles tissues. These values are lower than the commonly assumed average 3.4 discrimination factors reported for whole body and muscle isotopic analyses. The increasing reliance on noninvasive and non -destructive sampling in animal isotopic ecology requires that we recognize and understand why different tissues differ in is otopic discrimination factors.
77 Results of my study of isotopic discrimination and incorporation in hatchling and juvenile t urtles provide a baseline for interpreting data from studies of sea turtles that incorp orate stable isotope analysis. These data also provide a tool for experimental design, allowing scientists to identify and subsequently collect the tissue that will prov ide diet and habitat data f or the time frame of interest. Solving a M ystery Lost Years of Small Green Turtles The tissue with the greatest potential to provide scientists with data of historical diet and habitat use is scute, the keratinized epidermal tissue that for ms the outer layer of the carapace E ach scute is comprised of multiple layers that are deposited over time Because keratin is inert once deposited, there is no change in isotopic signature of the t issue once it has been formed. Because scute is continuously growing it can be used to evaluate the ecolo gy of inaccessible lifestages. In Chapter 3, I report how through the use of stable isotopes and the development of a new technique, I have answered a question that has plagued sea turtle biol ogists for decades Archie Carrs quest to locate the developmental habitat of small juvenile green turtles, Chelonia mydas, began more than 50 years ago (Carr 1952) After extensive surveys in neritic waters yielded no sightings, Carr (19 87) hypothesized that green turtle post -hatchlings spend their first years in oceanic habitats feeding primarily on invertebrates such as sea jellies and salps before shifting to feed on seagrasse s and algae in neritic waters. I used stable isoto pes to test Carrs conjecture. Analyses of stable carbon and nitrogen isotopes in successive layers of scute from small, neritic green turtles documented a clear ontogenetic shift in diet and habitat. Turtles were primarily carnivorous and oceanic early in life and changed to an herbivorous diet in neritic habitats as the y grew. My data provide strong support for Carrs long -standing conjecture.
78 Loggerhead Life History A New Perspective In Chapter 4, I used stable isotopes of carbon and nitrogen to invest igate the diet of Floridas nesting loggerhead population; this study yielded unexpected results. Analyses of skin samples for stable carbon and nitrogen isotopes were used to evaluate diet and habitat use of female loggerhead turtles (n=310) prior to thei r arrival at nesting beaches in Florida. Samples were collected in 2003 and 2004 from turtles nesting at four locations on the east coast of Florida I simultaneously collected samples of invertebrates on the carapaces of nesting turtles (n=52) from one of the sites Canaveral National Seashore. My results indicate that prior to the breeding season, loggerheads nesting in Florida use two different foraging strategies with females nearly equally distributed between oceanic and neritic foraging groups. These results are further supported by analysis of the epibiont communities collected from the carapace of 52 of the 310 turtles sampled. The differential distribution of oceanic and neritic epibionts on loggerheads with oceanic and neritic stable isotope signa tures confirms the bimodal foraging pattern. My study is the first to use epibionts to evaluate the bimodal for aging strategy in loggerheads. Because of the large size of this loggerhead population and the large proportion of oceanic foragers, these findin gs imply a major paradigm shift in the perceived roles of loggerheads in marine ecosystems and in the appropriate management plans for the conservation of this endangered species. Future R esearch N eeded Studies to Improve O ur A bility to U se S table I sotope Analyses in Sea Turtle Biology A global effort is needed to standardize the methodology of sample collection, preservation, preparation and analysis use of stable isotope analysis to study sea turtles We need to know the e ffect of common ly used preservation and lipid extraction methods on specific tissues Another gap in our knowledge is the degree of homogeneity within a given tissue ; i.e.,
79 we need to d etermin e i f skin from the shoulder area of a turtle is homogeneous within the shoulder area, o r with skin from other location s such as the flipper s Th ese data would contribute to the establishment of a global protocol, both in determining an optimum si te for collection of skin samples and in the method of homogenizing the sample in preparation for analysis. To interpret more accurately the isotopic discrimination factors and isotopic turnover in each species of sea turtle, isotopic incorporation and discrimination studies are needed for the six remaining species. Studies t o Advance O ur Knowledge o f Sea T urtle s and O ur A bi lity to Conserve Them Stable isotope analys e s when used in conjunction with existing methods of tracking sea turtles (satellite and radio telemetry, mark recapture tagging programs ) can aid scientists by providing a dietary histor y to accompany the geographical data collected via satellite or recovery of a previously tagged turtle. For example satellite telemetry is capable of reporting the number, depth and durat ion of dives made by a turtle. What it cannot provide are data on what the turtle was doing during and between dives is the dive made for the purpose of foraging? Or is the turtle foraging while at the surface? Stable isotope analysis can provide data on what habitat the turtle is using to forage, benthic or pel agic. Another long standing question about sea turtle biology is do nesting females forage during the inter nesting period? Analyses of serial samples of plasma collected from individual females across a nesting season may provide the answer to that ques tion. We also need to be able to compare stable isotope analysis of diet and trophic level of populations from different regions. Because the carbon and nitrogen values of organisms that form the base of the food web (i.e. plankton, POM -particulate organi c matter) differ between ocean basins (Wallace et al. 2006), it is important that we include analyses of turtles potential prey, plankton, and POM samples in our studies of sea turtle diet and habitat use. These data will contribute to the construction of an isoscape (map of baseline carbon and
80 nitrogen values) of marine study sites and facilitate accurate diet, habitat and trophic level comparisons of sea turtle populations across the globe. Development of management and conservation policy relies on rese arch to identify critical habitats, and to identify the role of these threatened and endangered animals in marine systems We need to know wh at habitat each lifestage occupies how long they stay, and what they do there. M y research has shown that stable i sotopes can aid scientists in attain ing this goal.
81 LIST OF REFERENCES Abreu Grobois A, Horrocks J, Formia A, Dutton P, LeRoux R, Vlez Zuazo X Soares L Meylan P (2006) New mtDNA Dloop primers which work for a variety of marine turtle species may increase the resolution of mixed stock analyses. In: Frick M, Panagopoulou A, Rees AF Williams K (compilers) Book of Abstracts. Twenty Sixth Annual Symposium on Sea Turtle Biology and Conservation. International Sea Turtle Society, Athens, Greece pp 179 Aoki M (1997) Comparative study of mother young association in caprellid amphipods: is maternal care effective? J Crustac Biol 17:447458 Addison DS Gore JA, Ryder J, Worley K (2002) Tracking post nesting movements of loggerhead turtles ( Caretta caretta ) with sonic and radio telemetry on the south west coast of Florida, USA. Mar Biol 141:201205 Adkins JN, Varnum SM, Auberry KJ, Moore KJ, Angell NH, Smith RD, Springer LD, Pounds JG (2002) Toward a human blood serum proteome: Analysi s by multidimensional separation coupled with mass spectrometry. Mol Cell Proteomics 1:947955 Araujo MS, Bolnick DI, Machado G, Giaretta AA, dos Reis SF (2007) Using 13C stable isotopes to quantify individual -level diet variation. Oecologia 152: 643654 Baldwin R, Hughes GR, Prince RIT (2003) Loggerhead turtles in the Indian Ocean. In: Bolten AB and Witherington BE (eds) Loggerhead sea turtles. Smithsonian Books, Washington, DC, pp 218 232 Barrick RE, Fischer AG, Showers WJ (1999) Oxygen isotopes from turt le bone: Applications for terrest rial paleoclimates? Palaios 14: 186191 Best PB, Schell DM (1996) Stable isotopes in southern right whale ( Eubalaena australis ) baleen as indicators of seasonal movements, feeding and growth. Mar Biol 124:483 494 Bjorndal KA (1997) Foraging ecology and nutrition of sea turtles. In: Lutz PL and Musick JA (eds), The biology of sea turtles. CRC Press, Boca Raton, Fl, pp 199231 Bjorndal KA (2003) Roles of loggerhead sea turtles in marine ecosystems. In: Bolten AB and Witheringt on BE (eds) Loggerhead sea turtles. Smithsonian Institution Press, Washington, DC, pp 235254 Bjorndal KA Bolten AB (2008) Annual variation in source contributions to a mixed stock: implications for quantifying connectivity. Mol Ecol 17:2185 2193 Bjorndal, KA, Bolten AB, Chaloupka MY (2005) Evaluating trends in abundance of immature green turtles, Chelonia mydas in the Greater Caribbean. Ecol Appl 15:304-314
82 Bjorndal KA, Bolten AB, Dellinger T, Delgado C, Martins HR (2003) Compensatory growth in oceanic loggerhead sea turtles: response to a stochastic environment. Ecology 84:12371249 Bjorndal KA, Bolten AB, Martins HR (2000) Somatic growth model of juvenile loggerhead sea turtles Caretta caretta : duration of pelagic stage. Mar Ecol Prog Ser 202:265272 Bolten AB, Balazs GH (1995 ) Biology of the early pelagic stage the lost year. In: Bjorndal KA (ed) Biology and conservation of sea turtles (Revised Edition) Smithsonian Institution Press, Washington DC, pp 579681 Bolten AB, Bjorndal KA, Martins HR, Dellinger T, Biscoito MJ, Encalada SE, Bowen BW (1998) Transatlantic developmental migrations of loggerhead sea turtles demonstrated by mtDNA sequence analysis. Ecol Appl 8:1 7 Bolten AB (2003a) Active swimmers passive drifters: the oceanic juvenile stag e of loggerheads in the Atlantic system. In: Bolten AB and Witherington BE (eds) Loggerhead sea turtles. Smithsonian Institution Press, Washington, DC, pp 6378 Bolten AB (2003b) Variation in sea turtle life history patterns: neritic vs. oceanic developmen tal stages. In: Lutz PL, Musick J and Wyneken J (eds), The biology of sea turtles, volum e II. CRC Press, Boca Raton, Fl pp 243257 Bolten AB (2007) The natural history and modern challenges of the North Atlantic loggerhead. State of the Worlds Sea Turt les (SWOT) Report, volume II. online access: http://seaturtlestatus.org/pdf/r2_naturalhisto ry.pdf Brown JH, West GB, Enquist BJ (2000) Scaling in biology: patterns, processes, causes, and consequences. In: Brown JH, West GB (eds) Scaling in biology. Oxford University Press, NY, pp 1 24 Burnham KP, Anderson DR (2002) Model selection and multimod el inference. Springer, NY Caine EA (1986) Carapace epibionts of nesting loggerhead sea turtles: Atlantic Coast of U.S.A. J Exp Mar Biol Ecol 95:1526 Carleton SA, Martnez del Rio C (2005) The effect of cold induced i ncreased metabolic rate on the rate of 13C and 15N incorporation in house sparrows ( Passer domesticus). Oecologia 114:226232 Carr A (1952) Handbook of Turtles: The Turtles of the United States, Canada, and Baja California Cornell University Press, Ithaca, NY Carr A (1986) Rips, FADS, and little loggerheads. Bio S cience 36:92101 Carr A (1987) New perspectives on the pelagic stage of sea turtle development. Conserv Biol 1:103121
83 Castanet J (1994) Age estimation and longevit y in reptiles. Gerontology 40:174192 Chamberlain CP, Blum JD, Holmes RT, Feng X, Sherry TW, Graves GR (1997) The use of isotope tracers for identifying populations of migratory birds. Oecologia 109: 132141 Cherel Y, Hobson KA, Weimerskirch H (2000) Using stable isotope analysis of feathers to distinguish moulting and breeding orig ins of seabirds. Oecologia 122: 155162 Crawley MJ (2002) Statistical computing: an introduction to data analysis in S -Plus. John Wiley & Sons, West Sussex, UK Cerling TE and Harri s JM (1999) Carbon isotope fractionation between diet and bioapatite in ungulate mammals and implications for ecological and paleontological studies. Oecologia 120:347 363 Cerling TE, Wittemyer G, Rasmussen HB, Vollrath F, Cerling CE, Robinson TJ, Douglas Hamilton I (2006) Stable isotopes in elephant hair document migration patterns and diet changes. Proc Natl Acad Sci US A 103:371373 Chace FA (1951) The oceanic crabs of the genera Planes and Pachygrapus Proc US Natl Mus 101:65103 Crawley MJ (2002) Statist ical computing: an introduction to data analysis in S -Plus. John Wiley & Sons, West Sussex, UK Dalerum F, Angerbjorn A (2005) Resolving temporal variation in vertebrate diets using naturally occurring stable isotopes. Oecologia 144:647658 DeNiro MJ, Epstein S (1978) Influence of diet on the distribution of carbon isotopes in animals. Geochim Cosmochim Acta 42:495506 DeNiro MJ, Epstein S (1981) Influence of diet on the distribution of nitrogen isotopes in animals. Geochim Cosmochim Acta 45:341351 Dod ds ED, McCoy MR, Geldenhuys A, Rea LD, Kennish JM (2004) Microscale recovery of total lipids from fish tissue by accelerated solvent extraction. J Am Oil Chem Soc 81:835 840 Eckert SA, Martins HR (1989) Transatlantic travel by juvenile loggerhead turtle. Mar Turt News 45:15 Ehrhart LM, Bagley DA, Redfoot WE (2003) Loggerhead turtles in the Atlantic Ocean: Geographic distribution, abundance, and population status. In: Bolten AB and Witherington BE (eds) Loggerhead sea turtles. Smithsonian Institution Press, Washington, DC, pp 157 174 Fogel ML, Tuross N (2003) Extending the limits of paleodietary studies in humans with compound specific carbon isotope analysis of amino acids. J Archaeol Sci 30:535545
84 Foster JM, Thoma BP, Heard RM (2004) Range extensions and review of the caprellid amphipods (Crustacea: Amphipoda: Caprellidae) from the shallow, coastal waters from the Suwannee River, Florida, to Port Aransas, Texas, with an illustrated key. Gulf Carib Res 16:161175 France RL (1995) Carbon13 enrichment in ben thic compared to planktonic algae: foodweb implications. Mar Ecol Prog Ser 124:307312 Frick MG, Williams KL, Robinson M (1998) Epibionts associated with nesting loggerhead sea turtles ( Caretta caretta ) in Georgia, USA. Herpetol Rev 29: 211 214 Frick MG, Ross A, Williams KL, Bolten AB, Bjorndal KA, Martins HR (2003) Epibiotic associates of oceanic -stage loggerhead turtles from the southeastern North Atlantic. Mar Turt News 101: 1820 Frick MG, Ross A, Williams KL, Bolten AB, Bjorndal KA, Martins HR (2004) D iet and fecundity of columbus crabs, Planes minutus associated with oceanic-stage loggerhead sea turtles, Caretta caretta and inanimate flotsam J Crustac Biol 24:350355 Frick MG, Williams KL, Bresette M, Singewald DA, Herren RM (2006) On the occurrence of Columbus Crabs ( Planes minutus ) from loggerhead turtles in Florida, USA. Mar Turt News 114 :12 14 Fry B, Arnold C (1982) Rapid 13C/12C turnover during growth of brown shrimp ( Penaeus aztecus ). Oecologia 54:200204 Gannes LZ, Martnez del Rio C, Koch P (1998) Natural abundance variations in stable isotopes and their potential uses in animal physiological ecology. Comp Biochem Phys 119: 7257 Gaye -Seisseggar J, Focken U, Abel H, Becker K (2003) Feeding level and diet qua lity influence trophic shift of C and N in Nile tilapia ( Oreocromis niloticus (L.)). Isot Environ Health Stud ies 39:125134 Gaye -Seisseggar J, Focken U, Muetzel S, Abel H, Becker K (2004) Feeding level and individual metabolic rate affect 13C and 15N val ues in carp: implications for food web studies. Oecologia 138:175183 Godley BJ, Thompson DR, Waldron S, Furness RW (1998) The trophic status of marine turtles as determined by stable isotope analysis. Mar Ecol Prog Ser 166:277284 Godley BJ, Richardson S, Broderick AC, Coyne MS, Glen F, Hays GC (2002) Long term satellite telemetry of the movements and habitat utilization by green turtles in the Mediterranean. Ecography 25: 352362 Gustafson L, Showers W, Kwak T, Levine J, Stoktof M (2007) Temporal and spat ial variability in stable isotope compositions of a fresh water mussel: implications for biomonitoring and ecological studies. Oecologia 152:140150
85 Harrison AL, Bjorndal KA (2006) Connectivity and wide -ranging species in the ocean. In: Crooks KR and Sanj ayan MA (eds) Connectivity conservation. Cambridge University Press, Cambridge pp 213 232 Haschemeyer AEV, Smith MAK (1979) Protein synthesis in liver, muscle, and gill of mullet (Mugil cephalus L.) in vivo. Biol Bull 156:93102 Hatase H, Takai N Matsuzawa Y Sakamoto W, Omuta K Goto K Arai N, Fujiwara T (2002a) Size -related differences in feeding habitat use of adult female loggerhead turtles Caretta caretta around Japan determined by stable isotope analyses and satellite telemetry. Mar Ecol Pro g Ser 233:273281 Hatase H, Matsuzawa Y Sakamoto W Baba N Miyawaki I (2002b) Pelagic habitat use of an adult Japanese male loggerhead turtle Caretta caretta examined by the Argos satellite system. Fish Sci 68:945947 Hatase H, Matsuzawa Y Sato K Bando T Goto K (2004) Remigration and growth of loggerhead turtles ( Caretta caretta ) nesting on Senri Beach in Minabe, Japan: life history polymorphism in a sea turtle population. Mar Biol 144:807811 Hawkes LA Broderick AC Coyne MS Godfrey MS Lopez Jurado LF Lopez -Suarez P Merino SE Varo -Cruz N Godley BJ (2006) Phenotypically linked dichotomy in sea turtle foraging requires multiple conservation approaches. Curr Biol 16:990995 Hayes GC, Akesson S, Broderick AC, Glen F, Godley BJ, Luschi P, Martin C, M etcalfe JD, Papi F (2001) The diving behavior of green turtles undertaking oceanic migration to and from Ascension Island: dive durations, dive profiles, and depth distribution. J Exp Biol 204: 40934098 Hesslein RH, Hallard KA, Ramal P (1993) Replacement of sulfur, carbon, and nitrogen in tissue of growing broad whitefish ( Coregonus nasus ) in response to a change in diet traced by 34S, 13C, and 15N. Can J Fish Aquat Sci 50:20712076 Hobson KA (1999) Tracing origins and migration of wildlife using stable iso topes: a review. Oecologia 120: 397404 Hobson KA, Bairlein F (2003) Isotopic fractionation and turnover in captive garden warblers (Sylvia borin): implications for delineating dietary and migratory associations in wild passerines. Can J Zool 81:16301635 Hobson KA, Clark RG (1992) Assessing avian diets using stable isotopes I: turnover of 13C in tissues. Condor 94:181188 Hobson KA, Schell DM (1998) Stable carbon and nitrogen isotope patterns in baleen from eastern Arctic bowhead whales ( Balaena mystic etus ). Can J Fish Aquat Sci 55:26012607 Hobson KA, Stirling I (1997) Low variation in blood 13C among Hudson Bay polar bears: implications for metabolism and tracing terrestrial foraging. Mar Mamm Sci 13:359367
86 Hobson KA, Welch HE (1992) Determination of trophic relationships within a high Arctic 1315N analysis. Mar Ecol Prog Ser 84:9 18 Hobson K A, Alisaiskas RT C lark RG (1993) Stable -nitrogen isotope enrichment in avian tissues due to fasting and nutritional stress: implic ations for isotopic analysis of diet. Condor 95: 388394 Hobson KA, Piatt JF, Pitocchelli J (1994) Using stable isotopes to determine seabird trophic relationships. J Anim Ecol 63:786798 Houlihan DF, Carter CG, McCarthy I (1995) Protein turnover in animals In: Walsh PJ, Wright P (eds) Nitrogen metabolism and excretion. CRC Press, Boca Raton, FL. pp 1 -32 Howland MR, Corr LT, Young SMM, Jones V, Jim S, Van der Merwe NJ, Mitchell AD, Evershed RP (2003) Expression of the dietary isotope signal in the compound-specific 13C values of pig bone lipids and amino acids. Int J Osteoarchaeol 13:5465 Iverson JB (1984) Proportional skeletal mass in turtles. Fla Sci 47:111 Kelly JF (1999) Stable isotopes of carbon and nitrogen in the study of avian and mammalian trophic ecology. Can J Zool 78:1 27 Killingly JS, Lutcavage M (1983) Loggerhead turtle movements reconstructed from 18O and 13C profiles from commensal barnacle shells. Oecologia 16:345349 Kohler A (1964) Variation in the growth of Atlantic cod ( Gadus mor hua L.). J Fish Res B oard Can 21:57100 Kraemer JE, Bennett SH (1981) Utilization of posthatching yolk in loggerhead sea turtles, Caretta caretta Copeia 1981:406411 Lajtha K, Michener RH (1994) Stable isotopes in ecology and environmental science. Blackw ell Scientific Publications, Oxford UK Lesage V, Hammill MO, Kovacs KM (2002) Diet tissue fractionation of stable carbon and nitrogen in phocid seals. Mar Mamm Sci 18:182193 Lorian D, Erez J, Lazar B (1992) Stable carbon isotopes in the reef ecosystem of the Gulf of Eliat Red Sea. In: Achituv Y, Brickner L, Erez J (1997) Stable carbon isotope ratios in Red Sea barnacles ( Cirripedia ) as an indicator of their food source. Mar Biol 130:243247 MacAvoy SE, Macko SA, Garman GC (2001) Isotopic turnover in aquatic predators: quantifying the exploitation of migratory prey. Can J Fish Aquat Sci 56:923 932 MacAvoy SE, Macko SA, Arneson LS (2005) Growth versus metabolic tissue replacement in mouse tissues determined by stable carbon and nitrogen isotope analysis. C an J Zool 83:631 641
87 Macko SA, Estep MLF, Engel MH, Hare PE (1986) Kinetic Fractionation of stable isotopes during aminoacid transamination. Geochem Cosmochim Acta: 50:21432146 Martnez del Rio C, Wolf BO (2005) Mass b alance models for animal isotopic ecology. In: Starck MA, Wang T (eds) Physiological and ecological adaptations to feeding in vertebrates. Science Publishers, Enfield, NH, pp 141174 McCain JC (1968) The Caprellidae (Crustacea: Amphipoda) of the western No rth Atlantic. Bull US Natl Mus 278: 1 147 McClellan CM, Read AJ (2007) Complexity and variation in logge rhead sea turtle life history. Biol Lett 3:592 594 McClelland JW, Montoya JP (2002) Trophic relationships and the nitrogen isotopic composition of amino acids in plankton. Ecology 83:21732180 McCutchan JH Jr., Lewis WM Jr., Kendall C, McGraith CC (2003) Variation in trophic shift for stable isotope ratios of carbon, nitrogen, and sulfur. Oikos 102:378390 McIntyre PB, Flecker AS (2006) Rapid turnover of t issue nitrogen of primary consumers in tropical freshwaters. Oecologia 148:1221 Michener RH, Schell DM (1994) Stable isotope ratios as tracers in marine aquatic food webs. In: Lathja K, Michener RH (eds) Stable isotopes in ecology and environmental scienc e. Blackwell, NY, pp 138 157 Miller K, Birchard GF (2005) Influence of body size on shell mass in the ornate turtle, Terrapene ornate J Herpetol 39:158161 Minagawa M, Wada E (1984) Stepwise enrichment of 15N along food chains: further evidence and the re lation between 15N and animal age. Geochim Cosmochim Acta 48:11351140 Musick JA, Limpus CJ (1997) Habitat utilization and migration in juvenile sea turtles. I n Lutz PL, Musick JA (eds) The biology of sea t urtles CRC Press, Boca Raton, FL pp 137163 National Marine Fisheries Service and U.S. Fish and Wildlife Service (1991 ) Recovery plan for U.S. population of loggerhead turtle, Caretta caretta National Marine Fisheries Service, Washington, DC USA National Marine Fisheries Service and U.S. Fish and Wildlife Service (2008) Recovery plan for the Northwest Atlantic population of the loggerhead sea turtle ( Caretta caretta ), second revision. National Marine Fisheries Service, Silver Spring, MD, USA Newsome SD, Martnez del Rio C, Phillips DL, Bearhop S (2007) A niche for isotopi c ecology. Front Ecol Environ 5: 429436 OBrien DM, Schrag DP, Martnez del Rio C (2000) Allocation to reproduction in a hawkmoth: a quantitative analysis using stable isotopes. Ecology 81:282 2 2831
88 Pearson SF, Levey DJ, Greenberg CH, Martnez del Rio C (2003) Effects of elemental composition on the incorporation of dietary nitrogen and carbon isotopic signatures in an omnivorous songbird. Oecologia 135:5165 23 Perga ME, Gerdeaux D (2005) Are fish what they eat all year round? Oecologia 144:598606 Peterson BJ, Fry B (1987) Stable isotopes in ecosystem studies. Ann Rev Ecol Syst 18:293320 Pfaller JB, Bjorndal KA, Reich KJ, Williams KL, Frick MG (2006) Distr ibution patterns of epibionts on the carapace of loggerhead turtles, Caretta caretta. J Mar Biol Assoc UK Biodivers Rec 5381:14 Pfaller JB, Frick MG, Reich KJ, Williams KL, Bjorndal KA (2008) Carapace epibionts of loggerhead turtles ( Caretta caretta ) nesting at Canaver al National Seashore, Florida. J Nat Hist 1095 1102 Phillips DL, Eldridge PM (2006) Estimating the timing of diet shifts using stable isotopes. Oecologia 14:195203 Pinnegar JK, Polunin NVC (1999) Differential fractionation of 13C and 15N among fish tissues: implications for the study of trophic interactions. Funct Ecol 13:225231 Polovina JJ, Kobayashi DR, Parker DM Seki MP, Balaz s GH (2000) Turtles on the edge: movement of loggerhead turtles ( Caretta caretta ) along oceanic fronts, sp anning longline fishing grounds in the central North Pacific, 1997 1998. Fish Oceanogr 9:7182 Post DM (2002) Using stable isotopes to estimate trophic position: models, methods, and assumptions Ecology 83:703718 Reich KJ, Bjorndal KA, Bolten AB (2007) Th e lost years of green turtles: using stable isotopes to study cryptic lifestages. Biol Lett 3:712 714 Reich KJ, Bjorndal KA, nez del Rio C ( 2008) Effects of growth and tissue type on the kinetics of 13C and 15N incorporation in a rapidly growing ec totherm. Oecologia 155: 651663 Rooker JR, Secor DH, DeMetrio G, Kaufman AJ, Belmonte Rios A, Ticina V (2008) Evidence of trans -Atlantic movement and natal homing of bluefin tuna from stable isotopes in otoliths. Mar Ecol Prog Ser 368: 231239 Roth JD, Hob son KA (2000) Stable carbon and nitrogen isotopic fractionation between diet and tissues of captive red fox: implications for dietary reconstruction. Can J Zool 78:848852 Sakamoto W, Bando T, Arai N, Baba N (1997) Migration paths of the adult female and male loggerhead turtles Caretta caretta determined through satellite telemetry. Fish Sci 63:547 552
89 Schoeller DA (1999) Isotopic fractionation: why arent we what we eat? J Archaeol Sci 26:667 673 Sebens KP (1987) Th e ecology of indeterminate growth in animals. Annu Rev Ecol Syst 18:371 407 Seminoff JA, Resendiz A, Nichols WJ (2002) Home range of green turtles ( Chelonia mydas ) at a coastal foraging area in the Gulf of Californi a, Mexico. Mar Ecol Prog Ser 242:253265 Seminoff JA, Jones TT, Eguchi T, Jones DR, Dutton PH (2006) Stable isotope discrimination 1315N) between soft tissues of the green sea turtle Chelonia mydas and its diet. Mar Ecol Prog Ser 308:271278 Seney EE, Musick JA (2007) Historical diet ana lysis of loggerhead sea turtles ( Caretta caretta ) in Virginia. Copeia 2007:478489 Stuart A, Ord JK (1994) Kendalls advanced t heory of statistics. Volume 1, D istribution T heory. Edward Arnold, New York Sullivan JC, Buscetta KJ, Michener RH, Whitaker JO, Finnerty JR, Kunz TH (2006) Models developed from delta C 13 and delta N 15 of skin tissue indicate non-specific habitat use by the big brown bat ( Eptesicus fuscus ). Ecoscience 13:11 22 Swingle WM, Warmolts DI, Keinath JA, Musick JA (2005) Exceptional grow th rates of captive loggerhead turtles, Caretta caretta Zoo Biol 12:491 497 Tieszen LL, Boutton TW, Tesdahl KG, Slade NA (1983) Fractionation and turnover of stable 13C analysis of diet. Oecologia 57:3237 Tominga O, Uno N, Seikai T (2003) Influence of diet shift from formulated feed to live mysids 1315N in dorsal muscles of juvenile Japanese flounders, Paralichthys olivaceus Aquaculture 218:265276 Tsudaka K, Moriyama T, Lieberman I (1971) Liver amino acid pool may be homogeneous with respect to protein synthesis. J Biochem 70:173 174 Turner MW, Hulme B (1970) The plasma proteins: an introduction. Pitman Medical & Scientific Publishing Company, London Vanderklift MA, Ponsard S (2003) Sources of variation in consumer diet 15N enrichment: a meta analysis. Oecologia 136:169182 Wallace BP, Seminoff JA, Kilham SS, Spotila JR, Dutton PH (2006) Leatherback turtles as oceanographic indicators: stable isotope analyses reveal a trophic dichotomy between ocean basins. Mar Biol 149:953 960
90 West GB, Brown JH, Enquist BJ (2001) A general model for ontogenetic growth. Nature 413:628 631 Williams AB, (1984) Shrimps, lobsters and crabs of the Atlantic co ast of the eastern United States, Maine to Florida. Smithsonian Institution Press, Washington DC 550pp Witherington BE (2002) Ecology of neonate loggerhead turtles inhabiting lines of downwelling near a Gulf Stream front. Mar Biol 140:843853 Witherington B, Kubili s P, Brost B, Meylan A (2009) Decreasing annual nest counts in a globally important loggerhead sea turtle population. Ecol Appl 19: 3034 Wunder MB, Norris DR (2008) Improved estimates of certainty in stable isotope based methods for t racking migr atory animals. Ecol Appl 18:549559 Youngson AF, McLaren IS, Bacon PJ, Jones PJ, Jones W (2005) Seasonal growth patterns of wild juvenile fish: partitioning variation among explanatory variables based on individual growth trajectories. J Anim Ecol 74:1 11 Zaykin DV, Pudovkin AI (1993) Two programs to estimate chi square values using pseudoprobability test. J Hered 84:152 Zimmerman DL, Nez -Anton V, Gregoire TG, Schanenberger O, Hart JD, Kenward MG, Molenberghs G, Verbeke G, Pourahmadi M, Vieu P (2001) Par ametric modelling of growth curve data: an overview. TEST 10:1 73 Zug GR, Glor RE (1999) Estimates of age and growth in a population of green sea turtles (Chelonia mydas ) from the Indian River lagoon system, Florida: a skeletochronologial analysis. Can J Z ool 76:14971506.
91 BIOGRAPHICAL SKETCH Kimberly Reich was born in Delray Beach, Florida, USA, in 1960. In 1995 she returned to school in pursuit of a new career. Kimberly received her Bachelor of Science in b iology with a specialization in m arine b iology and a m inor in o ceanography in 1998. In January 1999, she entered the graduate program in the Department of Wildlife and Fisheries Scien ces at Texas A&M University in College Station, Texas. In August 2001 she graduated from Texas A&M University with her M.S. degree and entered the graduate program in the Department of Zoology at the University of Florida in Gainesville, Florida to pursue her Ph.D.