Title Page
 Table of Contents
 Introduction and review
 Materials and methods
 Discussion and conclusions
 Biographical sketch

Title: Use of adenoviral vector for studying transport kinetics of green fluorescent protein and inflammation in the facial nerve system of the rat
Full Citation
Permanent Link: http://ufdc.ufl.edu/UF00100679/00001
 Material Information
Title: Use of adenoviral vector for studying transport kinetics of green fluorescent protein and inflammation in the facial nerve system of the rat
Physical Description: vi, 31 p.
Language: English
Creator: Alter, Todd Matthew ( Dissertant )
Streit, W.J. ( Thesis advisor )
Hauswirth, W. ( Reviewer )
Goodenow, M. ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 2000
Subjects / Keywords: Molecular Genetics and Microbiology thesis, M.S   ( local )
Dissertations, Academic -- Molecular Genetics and Microbiology -- UF   ( local )
Genre: government publication (state, provincial, terriorial, dependent)   ( marcgt )
bibliography   ( marcgt )
theses   ( marcgt )
non-fiction   ( marcgt )
Abstract: Adenoviral vectors have been used in gene delivery studies throughout the nervous system. This study investigated the use of Ad.CMV-GFPh in the rat facial nerve system. The goals were to determine the optimal viral titer, transgenic GFPh kinetics, and inflammatory responses when the vector was administered at the peripheral injury site of the facial nerve system. My results indicate the optimal titer for in vivo transduction of this system to be 5e9 pfu/ml. Comparison of the brains of the control animals to the Ad.CMV-GFPh treated animals indicated no difference in reactive gliosis for the first 7 days post injury. Furthermore, the reactive gliosis in Ad.CMV-GFPh treated animals did not show selective gliosis for neurons expressing GFPh. At the peripheral injury site Ad.CMV-GFPh treated animals had increased mononuclear cell infiltration compared to the control animals, while both groups exhibited mast cell infiltration.
Subject: facial nerve, adenovirus, GFP, green fluorescent protein, gene delivery, inflammation
Thesis: Thesis (M.S.)--University of Florida, 2000.
Bibliography: Includes bibliographical references (p. 29-30).
System Details: System requirements: World Wide Web browser and PDF reader.
System Details: Mode of access: World Wide Web.
Statement of Responsibility: by Todd Matthew Alter.
General Note: Title from first page of PDF file.
General Note: Document formatted into pages; contains vi, 31 p.; also contains graphics.
General Note: Vita.
 Record Information
Bibliographic ID: UF00100679
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 81469485
alephbibnum - 002566162
notis - AMT2443


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Table of Contents
    Title Page
        Page i
        Page ii
        Page iii
    Table of Contents
        Page iv
        Page v
        Page vi
    Introduction and review
        Page 1
        Page 2
        Page 3
        Page 4
    Materials and methods
        Page 5
        Page 6
        Page 7
        Page 8
        Page 9
        Page 10
        Page 11
        Page 12
        Page 13
        Page 14
        Page 15
        Page 16
        Page 17
        Page 18
        Page 19
        Page 20
        Page 21
        Page 22
        Page 23
        Page 24
    Discussion and conclusions
        Page 25
        Page 26
        Page 27
        Page 28
        Page 29
        Page 30
    Biographical sketch
        Page 31
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