Group Title: Critical Care
Title: In contrast to CD64 expression, CD14 expression is persistently downregulated on polymorphonuclear cells (PMN) but not on monocytes of patients with septic shock
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Title: In contrast to CD64 expression, CD14 expression is persistently downregulated on polymorphonuclear cells (PMN) but not on monocytes of patients with septic shock
Series Title: Critical Care
Physical Description: Archival
Creator: Barth,E.
Wollmeyer,J.
Fischer,G.
Weckmann,T.
Appel,M.
Schneider,E. M.
Moldawer,L. L.
Georgieff,M.
Weiss,M.
Publication Date: 2000
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General Note: Start pageP53
General Note: M3: 10.1186/cc773
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Bibliographic ID: UF00100069
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: Open Access: http://www.biomedcentral.com/info/about/openaccess/
Resource Identifier: issn - 1364-8535

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Critical Care Vol 4 Suppl 1 20th International Symposium on Intensive Care and Emergency Medicine


P53 In contrast to CD64 expression, CD14 expression is persistently downregulated on
polymorphonuclear cells (PMN) but not on monocytes of patients with septic shock

E Barth, J Wollmeyer, G Fischer, T Weckmann, M Appel, EM Schneider, LL Moldawer*, M Georgieff and M Weiss
Department of Anesthesiology, University of Ulm, Steinhoevelstr. 9, 89070 Ulm, Germany; *Department of Surgery, University of
Florida, Gainesville, USA


Objectives: To investigate the time course of surface
CD14 and CD64 on PMN and on monocytes, in postoper-
ative/post-traumatic patients with septic shock, from the
first day of septic shock onwards.

Methods: Over a six month period, in 22 patients admit-
ted to the intensive care unit, a daily analysis of CD14 and
CD64 surface expression on PMN and monocytes was
performed by flow cytometry (Becton Dickinson, USA). All
patients suffered from septic shock for three days at least.
Box plots of the mean intensity (Mnl) values of surface
CD14 and CD64 are given for days 1-14. Statistical
analysis was performed by Wilcoxon rank sum test
(P<0.05; significant intragroup differences between the
day indicated by a star [*] and the following days denoted
by a line over the respective days [I-I]). In addition, normal
range of controls [C] is presented by a box plot.


Figure


PMN


D I D3 D5 D7 D9 Dl11 D13 C
D2 D4 D6 D8 D10 D 12 D14
PMN







D 1 D3 D5 D7 1D9 D1 1 D13 C
D2 D4 D6 D8 DI1G D 12 D14


Results: CD64 is significantly upregulated on PMN and
monocytes compared to healthy volunteers, whereas
CD14 expression is significantly decreased on all days.
Regarding the time course, a significant decrease in
CD14 and CD64 expression on PMN occurred within the
first three days. In monocytes, compared with the first two
days, CD64 expression was diminished from day 3
onwards, however, CD14 expression on day 4, only.

Conclusions: Upregulation of CD64 expression on PMN
and monocytes indicates leukocyte activation in patients
with septic shock. In contrast to CD64, surface CD14 on
PMN decreased and is continuously downregulated over
time, whereas no decrease in surface CD14 on mono-
cytes is observed. This may be due to different membrane
anchors and/or different LPS-CD14 signaling in PMN and
monocytes in patients during septic shock.




Monocytes

4000 T



1000 -T


500
400
300
CD 200
100


D1 D3 D5 D7 D9 D11 D13 C
D2 D4 D6 D8 D10 D12 D14
Monocytes







D1 D3 D5 D7 D9 D11 D13 C
D2 D4 D6 D8 D10 D12 D14


P54 Respiratory burst activity of PMN is increased in most patients during septic shock

E Barth, G Fischer, F Borner, LL Moldawer*, EM Schneider, M Georgieff and M Weiss
Department of Anesthesiology, University of Ulm, Steinhoevelstr. 9, 89070 Ulm, Germany; *Department of Surgery, University of
Florida, Gainesville, USA


Objectives: To investigate whether burst activity of poly-
morphonuclear cells (PMN) of postoperative/post-trau-
matic patients during septic shock is predominantly
increased, unchanged or decreased compared to that of
healthy volunteers.


Methods: Over a period of six months, 12 patients (four
survivors and eight nonsurvivors) with septic shock for at
least three days duration were studied during their period
of septic shock on the intensive care unit. E. coli induced
burst activity of granulocytes was determined by flow




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