• TABLE OF CONTENTS
HIDE
 Title Page
 Acknowledgement
 Table of Contents
 List of Tables
 List of Figures
 Abstract
 Introduction
 Background
 Methods and materials
 Results and discussion
 Summary and conclusions
 Appendices
 References
 Supplementary bibliography...
 Biographical sketch






Group Title: Trace element profile of B-16 murine melanoma by particle-induced x-ray emission analysis /
Title: Trace element profile of B-16 murine melanoma by particle-induced x-ray emission analysis
CITATION PDF VIEWER THUMBNAILS PAGE IMAGE ZOOMABLE
Full Citation
STANDARD VIEW MARC VIEW
Permanent Link: http://ufdc.ufl.edu/UF00099517/00001
 Material Information
Title: Trace element profile of B-16 murine melanoma by particle-induced x-ray emission analysis
Physical Description: x, 169 leaves : ill. ; 28 cm.
Language: English
Creator: Williams, Michael Duncan, 1941- ( Dissertant )
Roessler, Genevieve S. ( Thesis advisor )
Ohanian, M. J. ( Reviewer )
Van Rinsvelt, Henri A. ( Reviewer )
Weiner, Roy S. ( Reviewer )
Marks, Ronald G. ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1980
Copyright Date: 1980
 Subjects
Subjects / Keywords: Carcinogenesis   ( lcsh )
Nuclear Engineering Sciences thesis Ph. D   ( lcsh )
Cancer -- Research   ( lcsh )
Tumors -- Metastasis   ( lcsh )
Dissertations, Academic -- Nuclear Engineering Sciences -- UF   ( lcsh )
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
 Notes
Abstract: The primary objective of this research was to characterize the elemental composition of cancerous tissues, and specifically to investigate whether differences exist in the elemental concentrations of primary and metastasized tumor tissue. The analytical technique used was particle-induced x-ray emission analysis. The first step in achieving this objective was to produce the cancerous tumors in C57BL/6J black mice. B-16 murine melanoma was selected as the tumor model for this research because its biology is well documented in the literature and it has been used extensively in other metastasis research. Primary tumors developed at the site of the subcutaneous injections and metastatic lung tumors grew following the intraveneous injections. Approximately 30 days following the injections, the mice were sacrificed, and primary and metastatic tumor samples were collected and analyzed. Blood serum was also collected and analyzed to determine whether changes in serum levels could be correlated with the presence or extent of the disease. Some of the samples were split into two parts prior to analysis so that another research team could perform neutron activation analysis, a complementary analytical technique. Eight elements (potassium, calcium, iron, copper, zinc, selenium, bromine, and rubidium) were detected in all samples except serum where the rubidium concentration fell below the lower limit of detection. Five of the elements (iron, zinc, selenium, bromine, and rubidium) were observed by the two analytical methods. The results of both techniques corroborated for all elements except zinc. The Student's t test showed a significantly higher concentration of three elements (calcium, copper, and bromine) in the primary tumor compared to the metastatic tissue. In humans, calcium and bromine are two of the most abundant elements in the body. Calcium is utilized in the formation of bones and teeth, but bromine has not been found to be essential to proper health and nutrition. Their occurrence in higher proportions in the primary tumor may indicate that the body's defense mechanism uses these elements to combat the tumor. Copper is an essential element in humans and has been reported at elevated levels in the serum of certain cancer patients. Data from this research confirm the belief that copper is involved in cancer growth, but whether it is used by the body as a therapeutic agent or whether it is required by the rapidly dividing tumor is not known. This research has shown that elemental concentrations are different in primary and metastasized cancer tissues. Continued research is planned to further investigate differences in elemental content of cancer tissue.
Thesis: Thesis (Ph. D.)--University of Florida, 1980.
Bibliography: Bibliography: leaves 157-167.
General Note: Typescript.
General Note: Vita.
Statement of Responsibility: by Michael Duncan Williams.
 Record Information
Bibliographic ID: UF00099517
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000100255
oclc - 07334048
notis - AAL5716

Downloads

This item has the following downloads:

PDF ( 8 MBs ) ( PDF )


Table of Contents
    Title Page
        Page i
    Acknowledgement
        Page ii
        Page iii
    Table of Contents
        Page iv
        Page v
    List of Tables
        Page vi
    List of Figures
        Page vii
        Page viii
    Abstract
        Page ix
        Page x
    Introduction
        Page 1
        Page 2
        Page 3
        Page 4
        Page 5
        Page 6
    Background
        Page 7
        Page 8
        Page 9
        Page 10
        Page 11
        Page 12
        Page 13
        Page 14
        Page 15
        Page 16
        Page 17
        Page 18
        Page 19
        Page 20
        Page 21
        Page 22
        Page 23
        Page 24
        Page 25
        Page 26
        Page 27
        Page 28
        Page 29
        Page 30
    Methods and materials
        Page 31
        Page 32
        Page 33
        Page 34
        Page 35
        Page 36
        Page 37
        Page 38
        Page 39
        Page 40
        Page 41
        Page 42
        Page 43
        Page 44
        Page 45
        Page 46
        Page 47
        Page 48
        Page 49
        Page 50
        Page 51
        Page 52
        Page 53
        Page 54
        Page 55
        Page 56
        Page 57
        Page 58
        Page 59
        Page 60
        Page 61
        Page 62
        Page 63
        Page 64
        Page 65
        Page 66
        Page 67
        Page 68
        Page 69
        Page 70
        Page 71
        Page 72
        Page 73
        Page 74
        Page 75
        Page 76
        Page 77
        Page 78
        Page 79
        Page 80
        Page 81
        Page 82
        Page 83
        Page 84
        Page 85
        Page 86
        Page 87
        Page 88
        Page 89
        Page 90
        Page 91
        Page 92
        Page 93
        Page 94
        Page 95
        Page 96
        Page 97
        Page 98
        Page 99
        Page 100
        Page 101
        Page 102
        Page 103
        Page 104
        Page 105
        Page 106
        Page 107
        Page 108
        Page 109
        Page 110
        Page 111
        Page 112
        Page 113
        Page 114
        Page 115
        Page 116
    Results and discussion
        Page 117
        Page 118
        Page 119
        Page 120
        Page 121
        Page 122
        Page 123
        Page 124
        Page 125
        Page 126
        Page 127
        Page 128
        Page 129
        Page 130
        Page 131
    Summary and conclusions
        Page 132
        Page 133
        Page 134
    Appendices
        Page 135
        Page 136
        Page 137
        Page 138
        Page 139
        Page 140
        Page 141
        Page 142
        Page 143
        Page 144
        Page 145
        Page 146
        Page 147
        Page 148
        Page 149
        Page 150
        Page 151
        Page 152
        Page 153
        Page 154
        Page 155
        Page 156
    References
        Page 157
        Page 158
        Page 159
        Page 160
    Supplementary bibliography (Categorized)
        Page 161
        Page 162
        Page 163
        Page 164
        Page 165
        Page 166
        Page 167
    Biographical sketch
        Page 168
        Page 169
        Page 170
        Page 171
        Page 172
University of Florida Home Page
© 2004 - 2010 University of Florida George A. Smathers Libraries.
All rights reserved.

Acceptable Use, Copyright, and Disclaimer Statement
Last updated October 10, 2010 - Version 2.9.9 - mvs