Title: Characterization of Agrobacterium tumefaciens adsorption to potato tissue
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Permanent Link: http://ufdc.ufl.edu/UF00099488/00001
 Material Information
Title: Characterization of Agrobacterium tumefaciens adsorption to potato tissue
Physical Description: vi, 134 leaves : ill. ; 28 cm.
Language: English
Creator: Kluepfel, Daniel Albert, 1955- ( Dissertant )
Peuppke, Steven G. ( Thesis advisor )
Stall, Robert E. ( Reviewer )
Mitchell, David J. ( Reviewer )
Gurley, William B. ( Reviewer )
Moudgil, Brij M. ( Reviewer )
Fry, Jack L. ( Degree grantor )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1984
Copyright Date: 1984
 Subjects
Subjects / Keywords: Agrobacterium tumefaciens   ( lcsh )
Phytopathogenic bacteria   ( lcsh )
Plant Pathology thesis Ph. D
Potatoes -- Diseases and pests   ( lcsh )
Dissertations, Academic -- Plant Pathology -- UF
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
 Notes
Abstract: The Freudlich adsorption isotherm accurately defines the adsorption of Aqrobacterium tumefaciens to potato tuber discs. This isotherm describes a system where the available binding sites are not saturated. The_A. tumefaciens isotherm was developed for inoculum densities that ranged from 10 ' to 10^ bacteria/ml of buffer. Adsorption is rapid with more than 2.8 X 10^ bacteria adsorbing per potato disc within 0.5 min. Aqrobacterium tumefaciens dissociates from the disc and reaches equilibrium within 3 to 4 hr at 6°C. The adsorption isotherm was examined over a more limited initial inoculum density at three different temperatures: 6°C, 28°C, 38°C. Analysis of these isotherm data with the Clausius-Clapeyron equation shows that the heat of adsorption is close to zero. These combined data suggest that the adsorption of _A. tumefaciens to potato tuber tissue is governed by physical adsorption. Optimum adsorption of A_. tumefaciens to potato tissue occurred at pH 7.2, and adsorption was reduced at either pH 4.5 or 9.5. Adsorption to hydrophobic plastic was insensitive to changes in pH. Conversely, the adsorption to hydrophilic plastic surfaces was optimum at pH 4.5 and decreased with increasing pH. The isoelectric point of A^. tumefaciens suspended in water is approximately pH 2.9, as determined by zeta potential measurements. Adsorption of _A. tumefaciens cells to potato tissue was greatly reduced by detergents at concentrations as low as 0.05% (v/v). Anionic, cationic and neutral detergents all were effective. The effect of agents that influence both hydrophobic and ionic forces is consistent with the proposed physisorption mechanism for A^. tumefaciens. Calcium chloride treatment of the bacteria or EGTA treatment of the potato tuber discs did not, however, significantly affect bacterial adsorption. When viewed with the scanning electron microscope, bacterial colonization of the potato surface was the same for both the virulent A. tumefaciens strain ACH5 and its heat-cured derivative ACH5C3. The only ultrastructural feature that distinguished tissues inoculated with the virulent strain from those inoculated with the avirulent strain was the appearance of tumors five days after inoculation with the former.
Statement of Responsibility: by Daniel Albert Kluepfel.
Thesis: Thesis (Ph. D.)--University of Florida, 1984.
Bibliography: Bibliography: leaves 120-133.
General Note: Typescript.
General Note: Vita.
 Record Information
Bibliographic ID: UF00099488
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000458331
oclc - 11494285
notis - ACM1116

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