Group Title: Uteroferrin : a mechanism of maternal to fetal iron transport in swine
Title: Uteroferrin
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 Material Information
Title: Uteroferrin : a mechanism of maternal to fetal iron transport in swine
Physical Description: xii, 180 leaves : ill. ; 28 cm.
Language: English
Creator: Ducsay, Charles Andrew, 1953- ( Dissertant )
Bazer, Fuller W. ( Thesis advisor )
Roberts, R. Michael ( Reviewer )
Sharp, Daniel C. ( Reviewer )
Collier, Robert J. ( Reviewer )
Ammerman, Clarence B. ( Reviewer )
Zachariah G. L. ( Degree grantor )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1980
Copyright Date: 1980
Subjects / Keywords: Maternal-fetal exchange   ( lcsh )
Animal Science thesis Ph. D
Swine -- Physiology   ( lcsh )
Swine -- Embryos   ( lcsh )
Iron -- Metabolism   ( lcsh )
Dissertations, Academic -- Animal Science -- UF
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
Abstract: Uteroferrin (UF) is an iron-containing, progesteroneinduced glycoprotein with an approximate molecular weight of 35,000. This protein is present in uterine secretions of non-pregnant swine between days 12 and 16 of the estrous cycle and is also observed in allantoic fluid and uterine secretions of pregnant swine between days 30 and 100 of gestation. This study was designed to examine the role of UF in maternal to fetal iron transport. Experiment I utilized 21 pregnant crossbred gilts which were hysterectomized either on day 30, 45, 60, 75, 90, 105 or 112 of gestation. Fetal fluids and tissues were collected and analyzed for changes in iron content and UF levels during gestation. Concentration of fetal liver (FL) and placental (PL) iron was affected by day of gestation (P < .05) as were total FL iron, total PL iron, allantoic fluid (ALF) iron concentration and total ALF iron (P < .01). The UF content of ALF (measured by acid phosphatase activity) was affected by day (P < .01). Placental UF concentration was quantified by acid phosphatase activity and was affected by day (P < .05). Concentration and total UF content of PL, as measured by radioimmunoassay, changed during gestation (P < .01). A temporal relationship was observed between iron content and UF levels in both ALF and PL. Experiment II examined effects of maternal iron treatment between days 40 and 60 of gestation on iron content and UF levels in conceptus fluids and tissues on day 90 of gestation. Eight gilts were bred and divided between two treatment groups: 1) Control—no supplemental iron injections; 2) Iron treatment—total of 22 mg iron dextran/kg body weight divided among five injections between days 40 and 60 of gestation. All gilts were hysterectomized on day 90 and fetal fluids and tissues collected. No differences were observed in iron content or UF levels in FL and ALF; however, concentration of UF in PL was higher (P < .01) in the iron treatment group. Total UF content of PL followed a similar trend but was not significant (P > .05). In experiment III, eight gilts were bred and assigned to treatment on day 30, 60, 90 or 105 of gestation. Three gilts were rendered unilaterally pregnant and assigned to treatment on day 60 of gestation. On the assigned day, 59 100yCi Fe was injected intravenously into each gilt. After 24 h, gilts were hysterectomized and fetal fluids and tissues collected. Radiolabeled Fe-UF was isolated from concentrated ALF and uterine flushings from day 60 unilaterally pregnant 59 gilts. Accumulation of Fe was also observed in fetal tissues on all days examined. In experiment IV, in vitro and in vivo absorption of macromolecules by day 60 allantoic epithelium was examined. Absorption of fluorescein labeled y globulin (FyG) , uteroferrin (FUF) and transferrin (FTF) was observed in allantoic epithelium incubated in vitro at 37 C for 30 minutes. Twenty- four hours after introduction of FyG into allantoic fluid sacs of pregnant gilts, uptake of FyG by allantoic epithelium was detected. When y globulin (yG) was introduced in a similar manner, yG was observed in fetal serum after 24 h. In summary, these experiments indicate a temporal relationship between iron content and UF levels in ALF and PL. Iron treatment of the dam during peak production of UF affected (P < .01) placental UF content, but had no effect on fetal iron levels. Uteroferrin appears to be the major iron-containing uterine secretory product during midgestation and allantoic epithelium is capable of absorbing macromolecules present in ALF. Uteroferrin appears to be involved in iron transport in swine during gestation.
Statement of Responsibility: by Charles Andrew Ducsay.
Thesis: Thesis (Ph. D.)--University of florida, 1980.
Bibliography: Bibliography: leaves 161-179.
Original Version: Typescript.
General Note: Vita.
 Record Information
Bibliographic ID: UF00099376
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000014541
oclc - 07373434
notis - AAB7768


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