Group Title: ingress of Pseudomonas alboprecipitans Rosen into sweet corn (Zea mays saccharata (Sturtevant) Bailey) in relation to stomatal aperture and infection court /
Title: The Ingress of Pseudomonas alboprecipitans Rosen into sweet corn (Zea mays saccharata (Sturtevant) Bailey) in relation to stomatal aperture and infection court
CITATION PDF VIEWER THUMBNAILS PAGE IMAGE ZOOMABLE
Full Citation
STANDARD VIEW MARC VIEW
Permanent Link: http://ufdc.ufl.edu/UF00099250/00001
 Material Information
Title: The Ingress of Pseudomonas alboprecipitans Rosen into sweet corn (Zea mays saccharata (Sturtevant) Bailey) in relation to stomatal aperture and infection court
Physical Description: vi, 82 leaves : ill. ; 28 cm.
Language: English
Creator: Gitaitis, Ronald David, 1950- ( Dissertant )
Strandberg, James O. ( Thesis advisor )
Stall, Robert E. ( Thesis advisor )
Berger, Richard D. ( Reviewer )
Mitchell, David J. ( Reviewer )
Humphreys, Thomas E. ( Reviewer )
Fry, Jack L. ( Degree grantor )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1979
Copyright Date: 1979
 Subjects
Subjects / Keywords: Pseudomonas alboprecipitans
Plant Pathology thesis Ph. D
Corn -- Diseases and pests   ( lcsh )
Sweet corn -- Diseases and pests   ( lcsh )
Dissertations, Academic -- Plant Pathology -- UF
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
 Notes
Abstract: Events in the infection of sweet corn by Pseudomonas albopreaipitans Rosen were studied to assist in the development of control procedures for bacterial leaf blight and stalk rot of corn (BLBSRC) . Examination of the infection process of P. albopreaipitans provided evidence that leaf areas in or not far removed from the corn-plant whorl were the most favorable sites for infection. The whorl was a favorable environment for bacteria; dew and guttation water collected from the whorl contained nutrients that supported the growth and replication of the BLBSRC pathogen. The movement of a red-pigmented marker bacterium, Serratia. mavoesaens Bizio , was monitored in corn plants over time. Movement of 5. mar-aesaens confirmed that when applied in the whorl, bacteria were capable of upward movement in leaf tissue as far as 12.0 cm. A more in-depth analysis of ingress through stripping film autoradiography was done with P. albopreaipitans that had been labeled with glucose- C. The labeled bacteria were aggregated around stomatal areas in whorl tissue. Furthermore, based upon additional evidence obtained with a scanning electron microscope (SEM) it was concluded that stomata were portals of ingress for P. albopreaipitans. Bacteria were observed in stomata and sub- stomatal chambers 9 when corn-plant whorls were exposed to 10 cells/ml for at least 320 min. Inoculations of the total plant with P. albopreaipitans resulted in the formation of lesions only in leaves .emerging from or in the whorl at time of inoculation. Furthermore, a local application of the bactericide phenylmercuric acetate (PMA) in the whorl protected corn plants from the bacteria. Treatment with PMA not only reduced the amount of leaf damage due to P. albopreaipitans but also altered the distribution of lesion area. However, because of the short duration of protection (24-48 hr) by PMA, it was concluded that chemical protection of susceptible tissue in the whorl was inefficient for a commercial operation. Knowledge of the events in the infection of corn was of limited value in the unsuccessful attempts to devise control procedures alternative to chemical control. Prospective biocontrol agents were evaluated in planta and in mixed cultures' with a streptomycin-resistant mutant (PA 73-31R) of P. albopreaipitans. It was concluded that none of the organisms tested would serve as a microbial antagonist to prevent or reduce BLBSRC. as 12.0 cm. A more in-depth analysis of ingress through stripping film autoradiography was done with P. albopreaipitans that had been labeled with glucose- C. The labeled bacteria were aggregated around stomatal areas in whorl tissue. Furthermore, based upon additional evidence obtained with a scanning electron microscope (SEM) it was concluded that stomata were portals of ingress for P. albopreaipitans. Bacteria were observed in stomata and sub- stomatal chambers 9 when corn-plant whorls were exposed to 10 cells/ml for at least 320 min. Inoculations of the total plant with P. albopreaipitans resulted in the formation of lesions only in leaves .emerging from or in the whorl at time of inoculation. Furthermore, a local application of the bactericide phenylmercuric acetate (PMA) in the whorl protected corn plants from the bacteria. Treatment with PMA not only reduced the amount of leaf damage due to P. albopreaipitans but also altered the distribution of lesion area. However, because of the short duration of protection (24-48 hr) by PMA, it was concluded that chemical protection of susceptible tissue in the whorl was inefficient for a commercial operation. Knowledge of the events in the infection of corn was of limited value in the unsuccessful attempts to devise control procedures alternative to chemical control. Prospective biocontrol agents were evaluated in planta and in mixed cultures' with a streptomycin-resistant mutant (PA 73-31R) of P. albopreaipitans. It was concluded that none of the organisms tested would serve as a microbial antagonist to prevent or reduce BLBSRC.
Thesis: Thesis--University of Florida.
Bibliography: Bibliography: leaves 76-81.
General Note: Typescript.
General Note: Vita.
Statement of Responsibility: by Ronald David Gitaitis.
 Record Information
Bibliographic ID: UF00099250
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000014210
oclc - 06300096
notis - AAB7409

Downloads

This item has the following downloads:

PDF ( 3 MBs ) ( PDF )


University of Florida Home Page
© 2004 - 2010 University of Florida George A. Smathers Libraries.
All rights reserved.

Acceptable Use, Copyright, and Disclaimer Statement
Last updated October 10, 2010 - Version 2.9.9 - mvs