Title: Ultraviolet light induced recovery in Escherichia Coli of radiation damaged bacteriophage deoxyribonucleic acid
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Permanent Link: http://ufdc.ufl.edu/UF00099140/00001
 Material Information
Title: Ultraviolet light induced recovery in Escherichia Coli of radiation damaged bacteriophage deoxyribonucleic acid
Physical Description: ix, 80 leaves : ill. ; 28 cm.
Language: English
Creator: Silber, John Randall, 1949- ( Dissertant )
Achey, Phillip M. ( Reviewer )
Boyce, Richard P. ( Reviewer )
Ingram, Lonnie O. ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1977
Copyright Date: 1977
 Subjects
Subjects / Keywords: Escherichia coli   ( lcsh )
DNA repair   ( lcsh )
Microbiology and Cell Science thesis Ph. D   ( local )
Dissertations, Academic -- Microbiology and Cell Science -- UF   ( local )
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
 Notes
Abstract: The ability of ultraviolet light (UV) 0X17^ single stranded and replicative form DNA molecules to produce whole phage when transfected into UV irradiated, calcium treated E. coli K12 hosts was investigated. When IT/ irradiated single and replicative form 0X1TU DNA molecules were transfected into UV irradiated wild type hosts, an enhanced survival of the phage producing ability of the DNA was observed over that seen when the transfection was performed with unirradiated wild type hosts. A similar increase in survival was also found for both types of 0X17 ; t DNA molecules when they were transfected into E. coli deficient in excision repair ( uvrA- , uvrB- or uvrC- ) or recombinational repair (recB- or recB- recC-). No enhanced survival was found with recA- or lex/V- E . coli hosts . The level of the increased phage producing ability for single stranded 0X17^ DNA was 1.3 to 1.7 times greater than that for the replicative form DNA in all genetic backgrounds which showed enhanced survival. These results suggest the existence of a UV inducible recovery system which participates in the recovery of UV irradiated 0X17^ DNA. This recovery system in dependent upon recA and I ox A regulated function.'; but is independent of excision and recombinational repair. The common genetic requirements for the increased survival of both forms of 0X11 h DNA suggest a common mechanism of recovery for both. The greater survival of the single stranded molecule indicates that it is more susceptible to inducible recovery than the replicative form molecule which may be due to the physical differences between the two. The ability of gamma irradiated 0X17'* single stranded and replicative form DNA to produce whole phage when transfected into a UV irradiated, calcium treated E. coli wild type host was also investigated. By adding appropriate radical scavengers to aqueous solutions of the DNA, it was possible to specify which of the water radiolysis radicals was interacting with the DNA. The maximal enhancement of phage producing ability for both types of DNA was observed under conditions which removed the hydroxyl radical allowing the hydrogen radical and the solvated electron to predominate. Scavenging conditions which removed the hydrogen radical and solvated electron as well as the hydroxyl radical resulted in an enhancement of survival only half as large as the maximum. The same was found to be true for scavenging conditions which removed the hydrogen radical and solvated electron only. These results demonstrate the existence of a UV inducible repair system which mediates in the recovery' of 0X17*1 DNA from gamma ray damage. The mechanism of recovery here could possibly be the same as that which affected the UV inducible recovery of UV irradiated 0X17 '4 DNA. From these results, it can also be concluded that the hydrogen radical and the solvated electron produce a type of damage that is more susceptible to inducible recovery than the hydroxyl radical. The hydroxyl radical appears to produce two classes of damage in both the single stranded and replicative form DNA molecules. The first is susceptible to inducible recovery while the second is refractory to it. This second class of damage may inhibit recovery from damage caused by the hydrogen radical and the solvatcd electron.
Thesis: Thesis--University of Florida.
Bibliography: Bibliography: leaves 72-79.
Statement of Responsibility: by John Randall Silber.
General Note: Typescript.
General Note: Vita.
 Record Information
Bibliographic ID: UF00099140
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000208052
oclc - 04086695
notis - AAX4856

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