Title: Unique morphogenetic and cell division behavior in populations of Escherichia coli treated with 6-amidinopenicillanic acid
Full Citation
Permanent Link: http://ufdc.ufl.edu/UF00099119/00001
 Material Information
Title: Unique morphogenetic and cell division behavior in populations of Escherichia coli treated with 6-amidinopenicillanic acid
Physical Description: vi, 70 leaves : ill., graphs ; 28 cm.
Language: English
Creator: Blalock, Harvey Antonio, 1944- ( Dissertant )
Previc, Edward P. ( Thesis advisor )
Bleiweis, Arnold S. ( Reviewer )
Proston, James F. ( Reviewer )
Shah, Dinesh ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1978
Copyright Date: 1978
Subjects / Keywords: Escherichia coli   ( lcsh )
Cell division   ( lcsh )
Bacterial cell walls   ( lcsh )
Amidinopenicillanic acid   ( lcsh )
Microbiology and Cell Science thesis Ph. D   ( local )
Cells -- Morphology   ( lcsh )
Dissertations, Academic -- Microbiology and Cell Science -- UF   ( local )
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
Abstract: A novel physiological system is described in Escherichia coli by means of which cellular morphology and division are perturbed in such a way that division occurs repeatedly at non-deterministic sizes and loci for a given exponential rate of mass increase. E. coli cells in balanced exponential growth, when treated with the novel penicillin derivative FL-1060 (Mecillinam, 6-amidinopenicillanic acid), assume an osmotically stable spherical configuration associated with retarded division. Under these conditions, dry weight of cells/ml and optical density at 450 nm increase exponentially at the same rate as in untreated cultures. Viable count and total particles per ml increase at a lower rate. This results in the development of a population of spheroid cells which divide symmetrically at non-characteristic volumes and (presumably) ages. Upon removal of excess antibiotic, either by washing of the cells, or by treatment with penicillinase, the anomalous morphology and division patterns persist for at least five divisions. The generation of a system in which the cycle of cell division is thus separated from the cycle of replication and distribution of cell constituents as measured by mass increase provides a potentially powerful probe of the control of both morphogenesis and division. One application of this probe is to determine whether or not the rate of synthesis of cellular envelope components is controlled in such a way as to maintain a quantity of envelope consistent with the altered surface-to-volume ratios accompanying a change from a cylindrical to spherical morphology. Experiments were done to measure the uptake and fixation of 2,6-diaminopimelic acid (DPM) into the cell-wall fraction of a culture of E. coli ATCC 13071, an absolute DPM auxotroph, in the presence and absence of FL-1060. It was found that cells in the spherical morphology accumulate labeled DPM at lesser rates than do untreated cells on the basis of mass. This difference does not conform to the surface-to-volume ratios expected.
Statement of Responsibility: by Harvey Antonio Blalock.
Thesis: Thesis--University of Florida.
Bibliography: Bibliography: leaves 67-69.
General Note: Typescript.
General Note: Vita.
 Record Information
Bibliographic ID: UF00099119
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000085292
oclc - 05317531
notis - AAK0641


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