Group Title: black flies (Diptera : Simuliidae) of Florida and their involvement in the transmission of Leucocytozoon smithi to turkeys
Title: The black flies (Diptera : Simuliidae) of Florida and their involvement in the transmission of Leucocytozoon smithi to turkeys
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 Material Information
Title: The black flies (Diptera : Simuliidae) of Florida and their involvement in the transmission of Leucocytozoon smithi to turkeys
Physical Description: xiii, 331 leaves : ill. ; 28 cm.
Language: English
Creator: Pinkovsky, Dennis Drew, 1947-
Copyright Date: 1976
 Subjects
Subject: Flies -- Florida   ( lcsh )
Entomology and Nematology thesis Ph. D
Dissertations, Academic -- Entomology and Nematology -- UF
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
 Notes
Statement of Responsibility: by Dennis Drew Pinkovsky.
Thesis: Thesis (Ph. D.)--University of Florida, 1976.
Bibliography: Bibliography: leaves 296-318.
General Note: Typescript.
General Note: Vita.
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Bibliographic ID: UF00098869
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000569107
notis - ACZ5866
oclc - 02526789

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THIE BAICK FLIES (DIPTERA:: SIHULLIIDAE) OF FLOIDAT, AN1\D 'THEIR
INVOLVEMENT INi THE TRANSMISSION OF'
L~eucocytozoon smith TO TURKEYS








By

DENNIS DREW PINKOVSKY


A D)ISSERTATIOJN PRESENTED TO THE GRA.DUAT~ COUNCIL OF
THE UNIVERSrTY OF FLOREDAl
IN PARTIAL FULFILLMENTT UF THE REQUIREMENTS FOR\ TbE
DEGREE OF DOCTOR OF PHILOSOPHti












UNIZVERSITY OF FLO;:rIDA
















ACKNOWLEDGEMIENTS


I sincerely thank Dr. J.F. Butler, my Committee Chairman, for his

constructive suggestions and support during my research endeavors and

for his valuable, critical review of my dissertation.

To Dr. D.F. Forrester I extend my deep appreciation for the advice

and guidance he enthusiastically offered during my transmission studies

and for the equipment and facilities he generously allowed me to use.

To all the mem~btrs of my Ph.D. Committee I express appreciation

for their critical appraisal of my dissertation and their helpful

comments.

I wish to thank Dr. E.L. Snoddy and Dr. G.E. Shewell for examining

black fly specimens which I sent from Florida and for the determinations

rendered.

I express my gratitude for the opportunity to examine black fly

specimens collected in Florida which were made available to me by

numerous individuals and institutions.

To the staff at the U.S. Department of Agriculture Insects Affecting

Man Laboratory, Gainesville, I extend my appreciation for the use of equip-

ment and for advice during my research and academic studies.

I am grateful to Dr. E.V. Komarek and the staff at the Tall Timbers

Research Station and L..E. pjilliams, D.H. Austin, and T. Peoples of the

Florida Game and! Fresh Water Fish~ Commission for the use of facilities

and other assistance during my collecting trips.









I wish to also thank the State of Florida Division of Recreation

and Parks for collecting permits and the opportunity to gather specimens

at the beautiful State Parks around Florida.

I extend my deep gratitude to the U.S. Air Force Institute of

Technology Civilian Institutions Program for financial support and for

the opportunity to attend the University of Florida and pursue my Ph.D.

degree.

To P. Humphrey, D. Young, L. DuBose and especially T. DiNuzzo I

express my sincere thanks for good-spirited support during my research.


iii




















































































General Comments .


Introduction to the Black Fly Keys. ..... .


A key to the larvae of the black flies of
Florida . . . . . *

A key to the pupae of the black flies of
Florida . . . . . . .


TABLE OF CONTENTS


ii


vi


vii


ziii


ACKNOWLEDGEMENTS. ....


LIST OF TABLES. ...c.


LIST OF FIGURES .....


AB STRACT. ........


CHAPTER


I. INTRODUCTION. ..........


II. LITERATURE REVIEW ........


Simuliidae. ...........


Taxonomy and Distribution. .
Bionomics. .........

Damage .. . .. . .. .
Control. ..........


Florida Ecological Habitats ...


Leucocytozoon smith. ......


III. MATERIALS AND METHODS ,.....


Black Fly Survey. ........


Leucocy-tozoon smithi Transmission


IV. RESULTS AND DISCUSSION. .....


Simuliidae. ...........


. .


. . .


. . .


. . .


. . .
. . .
. . .
. . .


. . .


. . .


. . .


. . .


. . .


. . .


. . .


. . .









TABLE OF CONTENTS
(Continued)


Page


A key to the adult male black flies of
Florida . . . . . . . .
A key to the adult female black flies of
Florida . . . . . . . . .

Introduction to the Individual Species Sections...
Chephia (Cnephia) ornithophilia Davies, Peterson,
and Wood . . . . . . . .
Simutiumr (Byssodon) meridionaie Riley. ......
Simulium (Bt~ssodon) slo~ssonae D~yar and Shannon ..
Sirmuium (Eurshnutium) cong~areenatum(Dyar and
Shannon) . . . . . . . . .
Simutium (Phosterodoros) dixieinse Stone and Snoddy
Simutium (Phosterodoros) haysi Stone and Snoddy..
Simu~ium (Phosterodoros) denningsi Malloch.. ..
Simutlium (Phosterodoros) jonesi Stone and Snoddy..
SimuZium (Phosterodoros) lake~i Snoddy.. .....
SimuZium (Phoster~odoros) notiale Stone and Snoddy.
SimuZium (Phosterodoros) nyssa Stone and Snoddy..
SimuZium (Phosterodoros) taxodium Snoddy and
Beshea-r. . . . . . . . .
Simu*Zium (Psilozia) vistatum Ze~ttrstedt
Simulium (Simutium) decorun Walker ........
Simulium (SimuZium) tuberosum (Lundstram)......
SimuZium (S~imulium) verecundwnr Stone and Jamnback..
Opephia species Undetermined No. 1.. ......
Shaulium species Undetermined No. 1.... ....

Leucocytozoon smith Transmission... ........

V. CONCLUSIONS.. ... .. .......

LITERATURE CITED. ...... ........

APPENDIX. COLLECTION SITE NAMES AND LOCATIONS........ .

BIOGRAPHICAL SKETCH.. ... .............
















LIST OF TABLES


>le Pag~e

1. Florida black fly species. .. .. .. .. .. ... 55

2. Florida black fly distribution records by county .. .. 57

3. Black fly associations based on! collections of immature
stages .. .. .. ... .. ... .. .. ... 59

4. Black flies captured in Manitoba traps . ... .. .. 60

5. Sentinel turkey locations and results. .. .. .. .. 278

6. Black flies captured in ramp traps .. .. .. .. .. 281

7. Blackout box trapping results. . .. ... .. ... 282

8. Black fly captures from exposed turkeys. .. .. .. .. 283

9. Leucocytozoon smith transmissions .. .. . ... 289
















LIST OF FIGURES


Igure Page

1. A modified Manitoba trap with a black plastic skirt . .. 44

2. Sentinel turkeys in an exposure cage. .. .. .. .. .. 47

3. A blackout box trap in the field. .. . .. ... ... 47

4. One view of a ramp trap .. .. .. ... ... .. 48

5. An exposed turkey in the field. .. .. ... .. .. 48

6. Glass container and paper cartons used for holding
black fly adults alive in the laboratory. .. .. .. .. 51

7. Locations in Florida where black flies have been
collected . .. .. . .. . .. .. .. 56

8. Seasonal occurrence of black flies in Florida .. ... 63

9. Dorsum of the head capsule of a black fly larva
(S. slossonae) .. .. .... .. .. .. .. .. 65

10. Venter of the head capsule of a black fly larva
(C.ornithoph~iia ... .. .. . .. .... .. .. 65

11. Lateral view of two black fly larvae (S. dirEense) .. .. 66

12. Black fly pupa and cocoon (S. diriense). .. .. . ... 66

13. A wing of the black fly onephia ornith~ophilia .. .. .. 68

14. A frontal view of the head of a female black fly
(S. notiate). .. .. .... .. .. . ... . .. 68

15. The male genitalia of a black fly, Cnephia
ornithoohilia ... .. .. .. . ... ... . .. 69

16. The distal portion of the hind leg of a S. meridionate
female. .. ... .. ... .. .. .. . .... 69

17. The terminalia of a female S. merZidonatea .. .. .. .. 70

18. The head spots of a larva of C. ornithophiflia . ... .. 82


vii










LIST OF FIGURES
(Continued)


Figure rage

19. The pupal exuvium and cocoon of C. ornithophilia. . ... 82

20. Tarsal claw of a female of C. ornithophilia .. .. .. 84

21. Genital fork and terminalia of a C. ornithophilia female. 84

22. Collection locations for C. ornithophilia in Florida. .. 88

23. Site 119, Guim Creek, a stream inhabited by
C. ornith~ophilia. .. . ... .. . . .89

24. The pupa and cocoon of S. mer~idionate .. ... . 94

25. The scutum of' a S. meridionale female . .. .. 94

26. Collection locations for S. meridionale in Florida. .. 97

27. Gular notch of a S. sZossoncae larva .. .. .. .. .. 101

28. The pupa and cocoon of S. slossonae . ... .. .. .. 101

29. Terminalia of a male of S. s~ossonae. .. .. .. .. .. 102

30. Terminalia of a female of S. sZossonae. .. .. .. .. 102

31. Collection locations for S. slossonzae in Florida. .. .. 105

3.Site 43, Double Run Creek, where S. so~;ssonae
immatures were collected. . .. .. .. .. .. 107

33. Cephalic apotome of a S. congareenarum larva. . .. ... 118

34. Venter of the larval head capsule of S. congareenaumo .. 118

35. Pupa and cocoon of S. congareenarum .. .. ... .. 120

36. Terminalia of a male of S. congareenarum. .. .. ... 120

37. Terminalia of a female of S. congaree~nawn. .. .. .. 121

38. Collection locations for S. congereencrum in Florida. .. 124

39. Site 216, Turkey Creek, a typical S. congareenarum
stream. .. .. ... .. .. .. .. .. .. ,.. 125

40. Dorsal view of the head capsule of a S. didcense larva. . 130

41. Gular notch and by~postomiumn of a S. d'ixiense larva. . .. 130









LIST OF FIGURES
(Continued)


vFigure.

42.

43.

44.

45.



46.

47.

48.

49.

50.


.. .132

. 132

. 134


Male terminalia of S. diziense. ..........

Terminalia of a female of S. dizciense .......

Collection locations for S. dizciense in Florida ..

Site 74, Pine Barrens Creek, a stream inhabited by
S. didcense . . . . . . . . . .


Cephalic apotome of a S. haysi larva. .......

Gular notch of a S. haysi larva ..........

Pupal exuvium and cocoon of S. haysi. .......

Location of the collection site for S. hacysi in Flol

Site 195, Juniper Creek, where S. haysi was collect~


. .135

. .138

..138

. .139

. .141

. .142


rida

ed .


51. Cephalic apotome of a S. jenningsi larva. .. .. ... 145

52. Gular notch of a S. jenningsi larva . ... .. .. .. 145

53. A pupa and cocoon of S. jenningsi .. .. .. .. .. .. 146

54. S. jenningsi male terminalia. .. ... .. .. .. .. 146

55. Genitalia of a female of S. jenningsi .. .. .. . 147

56. Collection locations for S. jenn~ingsi in Florida. . ... 151

57. Site 141 at Gulf Hamrmock where S. jennings~i was collected 152

58. Cephalic apotome of a S. jonesi larva ... .. .. 156

59. Gular notch of a S. jonesi larva. .. .. .. . .. 156

60. Respiratory organ of a S. jonesi pupa .. .. .. . 157

61. Terminalia of a male of S. jonesi .... .. .. .. 159

62. Genital fork and terminalia of a female of S. jon~esi. .. 159

63. Florida collection locations for S. jonesi. .. .. .. 161

64. Site 210, the Senholloway River, a S. jcnesi collection
location. ... .. .. .. .. .. .. .. .. .. 162










LIST OF FIGURES
(Continued)


Figure

65.

66.


67.

68.


69.

70.


71.


72.

73.

74.

75.


76.

77.


78.

79.


Page

171

171

172


172

. .173


. .173

175


. .176

. .183


...183

. .184

184

185


. .185

. .187


Cephalic apotome of a S. lakei larva. ........

Gular notch and hypostomium of a S. lakei larva ...


Pupa and cocoon of S. lakei .............

Dorsal view of a male of S. laket .....

Terminalia of a male of S. lakei....... .. .


Terminalia of a female of S. lakei. .........

Collection locations for S. lakei in Florida. ....


Site 135, Otter Creek, a collection site for S. lakei


Head spots of a S. notiale larva. ..........

Gular notch of a S. notiate larva ..........


Pupal exuvium and cocoon of S. notiale. .......

Scutum of a male of S. notiate. ...........


Terminalia of a male of S. notiale. .........

Terminalia of a female of S. notiaie. ........

Collection locations for S. notCate in Florida. ...


80. Site 88 at Chattahoochee where S. notiate immatures
were found. .. ... .. .. .. . .. .. . 188


81. S. nyssa pupa and cocoon. .. ... .. ... .. .. 191


82. Collection locations for S. nyssa in Florida. ... .. 193


83. Site 116, Blue Creek, where S. nyssa was collected. . .. 194

84. Cephalic apotome and head spots of a S. taxcodium larva. . 197

85. Gular notch of a S. tarcodium: larva. .. ... .. .. 197


86. Pupal exuvium and cocoon of S. taxcodiu7 . ... .. .. 198

87. Male terminalia of S. txodium. . .. .. .. ... .. 198


88. Female terminalia of S'. Laod~ium. .. .. .. .. .. .. 199




































































110. Terminalia of a female of S. tCuberosumr. .. ... . .. 239

111. Collection locations for S. tubrerosum in Florida. .. .. 243

112. Site 56 in Clay Countyr, a collection spot for S. tuberosum. 266


LIST OF FIGURtES
(Continued)


site. ...


Page

201

203

209

209

210

210

211

211

217

219

226

226

227

227

228

232

234

237

237

238

238


89. Collection locations for S. tax~odium in Florida

90. The Ichetucknee River, a S. taxcodium collection

91. Head spots of a S. vittatum larva .......


92.

93.

94.

95.-

96.

97.

98.

99.

100.

101.

102.

103.

104.

105.

106.

107.

108.

109.


Gular notch of a S. vittatum larva. ............

S. vittatum pupa and cocoon ................

Terminalia of a S. v-ittatum male. .............

Scutum of a female of S. vittatum.... .....

Terminalia of a female of S. vittatum... ....

Collection locations for S. vittatum1 in Florida ......

Site 167 at Crestview where S. vittatum was collected ...

Head spots of a S. decorum larva. .............

Gular notch of a S. deconrum larva .............

S. decorum pupa and cacoon. ................

Male terminalia of S. decoum ..... .....

Female terminalia of S. decorum ..............

Collection locations for S. decorum in Florida. ......

Shepard's Mill, Site 86, where S. decorum was collected ..

The cephalic apotome of a S. tuberosum larva. .......

The gular notch of a S. tuberosum larva ..........

Pupa and cocoon of S. tuberosum.... .....

Male terminalia of S. tuberosum.... .....









LIST OF FIGURES
(Continued)



Figure Page

113. The head spots of a S. verecundum larva. .. .. .. .. .257

114. The gular notch of a S. vereoundum larva .. . .. ... .257

115. The pupa and cocoon of S. verecundum .. . .. .. .258

116. Male terminalia of S. verecundum .. .. .. .. .258

117. Terminalia of a female of S. vereoundum. .. .. .. .. .259

118. Collection locations for S. verecundum in Florida. . .. .262

119. Panther Creek, Site 223, where S. verecundumlu was collected .264

120. Cephalic apotomce of a larva of Onephia species No. 1 .. .269

121. Venter of the head capsule of a larva of Cnephia species
No. 1... . .. .. .. .. . . .. .. .. 269

122. Collection location for Q~nephia species No. 1 in Florida .270

123. Pupal exuviumn and cocoon of SimuZi~um species No. 1 .. .273

124. Larval head spots of Simulium species No. 1. .. .. .. .274

125. Venter of the head capsule of the larva of SimuZium species
No. 1. .. .. .. .. .. .. .. ... .. ... .274

126. Collection location for SimuL~ium species No. 1 in Florida. .275

127. Small flow to Pine Barrens Creek, Site 74, where
Simulium species No. I was collected .. .. . .276

128. Gametocytes (G) of L. smith among normal turkey blood
cells. .. .. .. .. . . . .. .. .291

129. 00kinetes of L. smith .. .. .. . . .291

130. Sporozoites of L.. smith photographed in saline. . ... .292

131. Stained sporozoites of L. smithi . . .. .. . .292















Abstract of Dissertation Presented to the Graduate Council
of the University of Florida in Partial Fulfillment of the Requirements
for the Degree of Doctor of Philosophy



THE BLACK FLIES (DIPTERA: SIMULIIDAE) OF F~LORIDA AZND THEIR
INVOLVEMENT IN THIE TRANSMISSION OF
Leucocytozoon smith TO TURKEYS

By

Dennis Drew Pinkovsky

August, 1976


Chairman: Jerry F. Butler
Major Department: Entomology and Nematology

Immature and adult black flies (Diptera: Simuliidae) were collected

in Florida over a period of three years. Eighteen species of black

flies including ten which are new records for the State were found to

occur in Florida. Records of black flies from 192 locations in 50

counties are included. Biological information is provided for each

species together with data on distribution, seasonal occurrence, scream

ecology and species associations. Keys to the Simuliidae of Florida are

provided and structures are illustrated. Representative specimens have

been deposited in the Florida State Collection of Arthropods and in the

United States National Miuseum, Washington D.C.

Transmission investigations have incriminated three species,

SimuZiumi congareenrum~, S. me~ridion~ale, and S. slossonae, as vectors of

Leucoceytozon smithi to turkeys in Florida. On nineteen occasions

L. smithli was transmitted to domestic turkeys by the bites of infected

black flies.


xtiii














CHAPTER I
INTRODUCTION



Members of the family Simuliidae as immatures inhabit flowing water

and as adults are often blood feeders which may vector diseases to man

and animals in many parts of the world. A knowledge of the composition,

distribution, ecology, and habits of the simuliid fauna of any area of

local concern is essential for proper assessment of the impact on man of

these insects. Successful black fly control is dependent on thorough

knowledge of the major breeding sites, seasonal occurrence and other

facts concerning local Simuliidae.

When I arrived in Florida to begin my Ph.D. research I wrote to

the Florida Division of Health offices in Jacksonville, Vero Beach, and

Panama City and enquired about previous investigations on the black

flies of Florida. I found that little work had been done in the State

on this family of biting flies (Beck, 1973; Linley, 1973; Rogers, 1973 -

all personal communications). Mrs. A.T. Slosson collected black flies

in Florida (Dyar and Shannon, 1927), probably at the turn of the

twentieth century. Some of Mrs. Slosson's specimens and a portion of

those of Calvin Jones, Harry Couck, Darrell Anthony and other USDA

researchers who collected black flies in the northern and central por-

tions of the State in the 1940's and 1950's are located in the U.S.

National Museum. Based at least in part on the examination of

these specimens Stone (.1965.) listed the following seven species from

Florida: Crnephia (Cnephia) pecuarum (Riley); Simulium (Eusimuli~un)









congareenatnum(Dyar and Shannon); Simulium (Byssodon) meridionaite Riley;

Simuliwn, (Byssodon) saossonae Dyar and Shannon; Simulium (Simutium)

decorum Walker; SimuZitrm (Simulium) jenningsi Malloch; and Sirmutium

(Simutium) tuberosum (Lundstrijm). Stone and Snoddy (1969) did not list

S. jenningsi as occurring in Florida but did record two new species

Simulium (Phosterodoros) jonesi Stone and Snaddy and Simulium (Phostero-

d'oros) nyssa Stone and Snoddy from the State. Thus the total number

of species of black flies known to occur in Florida when I began my

research was eight.

This research was initiated in the fall of 1973 with the following

objectives: 1) to determine the species complement, distribution and

seasonal occurrence of black flies throughout Florida; 2) to gather

ecological information primarily on the immature stages; 3) to provide

keys with illustrations to the black flies of Florida; 4) to gather and

deposit in the U.S. National Museum of Natural History and the Florida

State Collection of Arthropods as complete a collection of adult and

immature Florida black flies as possible; and 5) to determine the vec-

tors of Leucocytozoon smith in turkeys in Florida.













CHAPTER II
LITERATURE REVIEW



Simuliidae


Taxonomy and Distribution


Rubtsov (1974) presents a concise discussion on the history and

major advances in black fly taxonomy. Linnaeus (1758) first described

two simuliids and assigned the names CuZez reptan~s and Culex: equinzus

but did not differentiate them from mosquitoes (Davies et al., 1962).

Latreille created the name Simulium for the genus in 1802 using Rhagio

cotomb~aschenzsis Fabricus as the type (Stone and Jamnback, 1955). This

remained the only genus for the thirty to forty black fly species de-

scribed up to the turn of the twentieth century. The work of Smith and

Kilbourne (1893) and others which focused on arthropods as vectors of

diseases stimulated much research on insects and related groups of

medical and veterinary importance. Roubaud (1906) designated two black

fly subgenera, Prosimuium and Eusinulium, based on differences in wing

venation. Lundstram (1911) in Sweden and Jobbins-Pomeroy (1916) in the

United States were early taxonomists who made use of the male terminalia

of simuliids to separate species. Edwards (1915) in England also used

male terminalia but more significantly in 1920 stressed the importance

of studying black fly larvae and pupae. Dyar and Shannon (1927) first

used female genitalia to separate North American black flies. Enderlein

(1930) divided the family Simuliidae into 29 genera. Smart (1945) took

a more conservative approach and distinguished 6 genera in the family:








ParasimuZium Malloch, ProsirmuLium Roubaud, Aulstrosimulium Tonnoir,

Gigantodcw: Enderlein, Onephia Edwards and Simulium Latreille. Stone

(1963) listed 11 genera and 22 subgenera in the Simuliidae which he

considered valid. Crosskey (1969) stressed a trinomial approach to

simuliid taxonomy with heavy reliance on subgenera. Rubtsov (1974)

recognized 17 genera of black flies in the Palaearctic region and listed

59 genera for the Simuliidae of the world, many of which American and

British authors regard as subgenera.

Brues et al. (1954) provide a worldwide bibliography of important

papers which deal with the taxonomy of the Simuliidae up to the early

1950's. In addition to the works already mentioned outstanding publi-

cations since about 1950 dealing with the classification and distribu-

tion of black flies outside of the United States include:


Palaearctic region Davies (1968) Britain, Carlsson (1969) Spain,

Rubtsov (1956 and 1962) U.S.S.R., Kuusela (1971) Finland,

Zivkovic (1971) Yugoslavia, Rivosecchi (1971 and 1972) Italy,

Crosskey and Pete~rson (1972), and Zwick (1974) Germany;

Ethiopian region Crosskey (1957) West Africa, Travis et al. (1967),

Crosskey (1969) Africa and its islands, Fain and Elsen (1973) -

Cameroons, Lewis and Raybould (1974) Tanzania;

Oriental region Travis and Labadan (1967a) Asia and European U.S.S.R.,

Delfinado (1969, 1971) Philippines, Crosskey (1973), Takaoka

(1973) Nansei Is., Uemoto et al. (1973) Japan, Lewis (1973) -

Pakistan, Datta (1973, 1974, 1975) India;

Australian Dumbleton (1963, 1972) Australia and New Zealand, Travis

et al. (1968);

Neotropical Leon and Wygodzinsky (1953) Ecuador, Dalmat (1955) -









Guatemala, Vulcano (1967), Travis and Labadan (1967b),Barreto

(1969) Colombia, Wygodzinsky and Coscaron (1970, 1973), Wygod-

zinsky and Najera (1970), Wygodzinsky (1971) Northern Andes,

Perez (1971) Venezuela, Rubtsov and Avila (1972) Cuba, Travis

et al. (1974);

Nearctic (excluding the U.S.) -- Syme and Davies (1958), Davies et al.

(1962) Ontario, Wood et al. (1963) Ontario, Travis et al. (1969),

Peterson (1970) Canada and Alaska, Lewis and Bennett (1973) -

Newfoundland.


In addition to the above works those of Rubtsov (1970, 1974) and

Coscaron and Wygodzinsky (1973) mention the variability which has been

observed in characters that are used for taxonomic determination of

black flies. Gambarian and Terterian (1973) applied a numerical tax-

onomy approach and using 100 characters tried to separate simuliids in

the Eusimulium group. The subgenus was found to be very homogeneous

and creation of supraspecific taxa was not statistically justified.

Internationally, black fly species determinations based on classical

external morphological characters and behavioral differences are being

supported by cytological, specifically chromosomal, studies. Rothfels

(1956) provided an introduction and overview on analytical techniques

for cytologically comparing black flies. Landau (1962), Dunbar (1969),

and Vajime and Dunbar (1975) used chromosomal differences to separate

forms or sibling species of a variety of simuliids including disease

vectors where previously each had been considered a single species.

Taxonomic works covering the whole United States include: Coquillett

(1898, 1902), Malloch (1914), Jobbins-Pomeroy (1916), Dyar and Shannon

(19217), and Stone (1965). Other publications were more regional in








scope. Western and midwestern articles include: RTinn (1938), Smith

and Lowe (1948) California; Nicholson and M~ickel (1950) Minnesota;

Stone (1952), Somrmerman (1953) Alaska; Wirth and Stone (1956) -

California; Stone and DeFoliart (1959); Anderson and Dicke (1960) -

Wisconsin; Stone and Boreham (1965), Hall (1972, 1974) California;

and Corredor (1975) Washington.

Northeastern U.S. works include: Johannsen (1903), Leonard (1926),

Metcalf (1932), DeFoliart (1951), Stone and Jamnback (1955), Jamnback

and Stone (1957), Jamnback (1969), Pinkovsky (1970), and, reporting the

unusual find of a South American black fly in the U.S. Wygodzinsky (1973)

- New York; O'Kane (1926) New Hampshire; Frost (1949) Pennsylvania;

Dimond and Hart (1953) Rhode Is.; Sutherland and Darsie (1960a and b)

- Delaware; Stone (1964) Connecticut; Holbrook (1967) Massachussetts;

Eckhart and Snetsinger (1969) Pennsylvania; and Crans and McCuiston

(1970a) New Jersey.

In the southeastern U.S. Tucker (1920) gave accounts of personal

experiences with black flies and reported two species, S. pecuarun and

S. meridionale, from Louisiana. Jones and Richey (1956) conducted a

survey of the black flies of Jasper County, South Carolina, and discussed

the biology, ecology and relationships to Leucocytozoon in turkeys of

one Cnephia (pecuarum) and seven Simulium species (congareenarum, deconk ,

jenningsi, slossonae, tuberosum, voenuatum and undescribed species).

Snow et al. (1958) reported on the ecology, habits and distribution of

black flies occurring in the Tennessee River Basin and recorded species

in the three genera: Chephia mutate2), Prosimutium~ (hirtipes, magnum,

plus undescribed species), and Silmu~ium (decorum, fibrinflatum~, jenningsi

ap. group, merid~ionate, pictipes, tuberose, veuan~tum, verecundum,





-7-


vittatum and three undescribed species). Snoddy and Hays (1966) mentioned

that eleven species of Simuliidae were captured at one location in

Alabama using a New Jersey light trap modified for daylight use by

removing the light and substituting,as an attractant, carbon dioxide

gas dispensed at .45 kg (1 lb)/hr. Stone and Snoddy (1969) present

distribution records, descriptions, and some biological information for

28 species of black flies discovered or expected to occur in Alabama.

Garris et al. (1975) present the seasonal distribution of 7 species of

Simulium collected from streams in Sumter Co., South Carolina, and

mention observations of black flies feeding on turkeys and captured in

a CO2-baited trap. Snoddy and Beshear (1968), and Snoddy (1971, 1976)

describe and give some facts on the biology of three new species

(S. tcaodiumi, podocstem~i and Zakei) in the expanding species list of the

former S. jenningsi group.



Bionomics


The eggs of black flies are dropped freely into the water or are

attached to substrates in the flows of streams and rivers. The larvae

which develop feed by filtering material from the current or by scraping

organic matter off the substrate and, usually within a month, transform

into pupae. From the pupa which is normally attached to rocks or

vegetation beneath the surface of the stream an adult fly emerges, in a

bubble of gas, rises to the surface and is immediately capable of flight.

The adult females of most black fly species suck blood and both male

and female flies obtain energy from natural sugar sources such as nectar

and honeydew.

Eggs. Ussova (1961) describes most black fly eggs as between .24








and .33 mm long and irregularly triangular in shape with rounded corners;

the eggs of some Cnephia species, however, are said to be larger and

elongate elliptical. Golini and Davies (1975) found black fly eggs were

.228 mm long and .139 mm wide. Davies and Peterson (1956) examined the

eggs of four genera (Gymnopais, ProsimuZium, Cnephia, Simutium) and

found no external sculpturing. The eggs are light in color when just

laid and darken as the embryos develop. Davies and Peterson (1956)

found that the eggs of Gymnopais and Prosimulium were the largest and

those of ProsimuZium and C~ep~hia were the narrowest of the genera checked.

Black fly eggs are often found attached to vegetation and rocks in a

swift flowing stream or river or lying free on the stream bottom.

Tarshis (1968) conducted experiments on and reviewed accounts in the

literature of desiccation and the overwintering of black fly eggs and

concluded, as Wu (1930) had done earlier, that only those eggs which

are kept moist, by damp stones or underground springs, etc., in ap-

parently dry stream bottoms and other situations are able to remain

viable. Tarshis (1968) found that freezing eggs of a number of species

at 0 to-700C killed the embryos, while he was able to maintain moistened

eggs alive for 424 days at 2-90C. Kurtak (1974) found that eggs of

SimuZ~ium pictipes, recovered while encased in ice from rock crevices

along a stream, hatched in the lab after three days in 100C flowing

water. In northern areas eggs of many black fly species hatch when the

water temperature reaches around 80C (Carlsson, 1967). Raybould and

Grunewald (1975) found eggs of the K~ibwezi form of S. down2osrum watch at

200C four days after oviposition. Tarshis (1968) indicates normal egg

hatch for Maryland black fly eggs occurs within one to five days.

Larvae general morph~ology and development Black fly larvae are

usually between 4.5 andl 10 mm long when mature, club-shaped with a wider









posterior end, and possess a pair of multiple-rayed cephalic fans on

the anterior end, an unsegmented ventral proleg in the thoracic region,

and a circlet of anal hooks posteriorly. Dark cephalic head spots mark

the origins of cephalic muscles. Tarshis (1968) found that larvae

hatched from eggs in the lab after 7-38 days at 1000 and 1-5 days at

20-250C. Larvae developed to pupae in 18-50 days at 100C and 11-29 days

at 20-250C. Cameron (1922), Dalmat (1955) and Reisen (1975) found six

larval instars in the black flies they studied. Johnson and Pengelly

(1970) observed S. rugglesi to pass through seven larval instars and

Fredeen (1975) mentioned a seventh and final instar for S. aration.

Craig (1975) distinguished nine larval instars in Tahitian species of

black flies.

Larval habitat. Larvae frequently attach by their posterior hooks

onto a patch of silk secreted by their large, looped salivary glands.

The silk is often applied to the same substrate on which the eggs are

found. Larvae may drift a short distance tethered to the old substrate

by a silk thread (Tarshis and Neil, 1970). A larva may also move in a

looping, geometrid fashion from silk patch to silk patch alternately

attaching its proleg then its anal disc (Dalmat, 1955). Elliot (1971)

reported that black fly larvae can actively migrate upstream as well as

passively downstream. Clean, smooth items free of algae or slime

are preferred attachment substrates for the larvae and larvae are

often located on such objects in stream sections where the current

is increased by a partial obstruction (Dalmat, 1955; Carlsson, 1967).

Larvae show positive phototaxis and colonize light substrates faster

and more densely than dark substrates (Carlsson, 1967). Unusual

attachment sites include the phoretic associations of black fly larvae

on prawns, mayfly nymphs, crabs, and other arthopods reported by









Disney (1971, 1973, 1975) and thle attachment of young larvae of S.

damnosum to older larvae of the same species (Burton, 1971). Carlsson

(1967) in Sweden found the greatest concentrations of larvae in flows

80 to 120 cm /sec although some species preferred 40

cm/sec currents. Cariase (1962) in the Philippines found immature black

flies concentrated where the water velocity was .5-.86 m/sec (1.63-2.83

ft/sec). Wu (1930) reported that simulfid larvae remained well estab-

lished at a velocity of 1.83 m/sec (6 ft/sec). Rohdendorf (1974) sug-

gested that the closed respiratory system of black fly larvae resulted

after invasion of the swift flowing well oxygenated habitat typical of

most simuliids. Tarshis (1968) found that black fly larvae could not

live more than eight hours in still water. Anderson and Shemanchuk (1975),

however, report they transported black fly larvae for several days in

shallow, non-agitated, ice-chilled water with little mortality. Wu

(1930) found approximately equal amounts of dissolved oxygen in quiet

and turbulent sections of black fly streams and after a number of ex-

periments concluded that black fly larvae have a definite requirement

for current to lessen sedimentation and supply adequate food, not because

of improved oxygen conditions. Crans and McCuiston (1970b)found larvae

Ln permanent rivers and streams, temporary creeks and flowing roadside

ditches. Dalmat (1955) and Lewis and Bennett (1975) reported finding a

few live larvae in situations where the flow was so slow that silt and

mud present prevented the larvae from anchoring to any fixed object.

Van Someren (1944) reported S. ruficorne larvae from small pools and

footprints in sandy river beds in Somaliland. Certain species are

typically found in limited type's of lotic habitats: S. arcticum in

large rivers (Cameron, 1922), S. pictipes at the outflow of damns and on









flat bedrock (Snow et al., 1958) and S. cohraceum in very small streams

about one meter (a few feet) wide and a few centimeters (a few inches)

deep (Dalmat, 1955). Travis and Vargas (1970) found that in contrast

to the clear mountain streams, the lower, slower Costa Rican streams

most polluted with sewage and garbage had the greatest larval and pupal

populations. Cariaso (1962) reported that black fly larvaeweredis-

couraged from breeding in water polluted with nitrogenous and human

wastes. Carlsson (1967) indicates moderate pollution increases organic

drift and is good for larvae but heavy pollution clogs the cephalic

fans. Streams in Wisconsin carrying large amounts of eroded soil par-

ticles and other detritus were observed to be poor habitats for most

black fly larvae due to feeding interference (Anderson and Dicke, 1960).

Larvae feeding structures, behavior, and food. Davies (1974)

and Craig (1974) describe the evolution and development of the important

larval feeding structures in many species, the cephalic fans, and men-

tion how these lateral palatal brushes which are well developed filter-

ing devices in most black flies are apomorphically absent in later

instars of Gyrmnopais and Zainnia and modified into raking structures

in Crosetia species. Chance (1970) stated that particles from about 1

to 350 microns in diameter, the majority in the 10 to 100 micron range,

were ingested by four filter feeding species she studied. Larvae were

found to attach within 10 centimeters of the water surface, extend their

bodies into the flow and alternately open and close the fans combing

particles from the fans when they were closed into the cibarium where

a bolus was formed. Mulla and Lacey (1976) report larvae, with their

heads pointed downstream, attach to silk deposits with their anal hooks,

rotate the body 900 to 1800 and open the cephalic fans to strain matter









from the flow. Chance (19703) suggests that the horizontally operating

mandibles, especially in non-filtering, grazing forms like Tannia,

scrape organic food off the substrate. Rubtsov (1974) discusses the

outer sclerite of the labium the submentum or hypostomum, an anteriorly

serrate structure bearing setae which lies just cephalad of the

gular notch and mentions that it is a tactile organ, is important in

producing the larval silk strands, and that it may serve to scrape food

from the substrate. In species which lack fans the submental teeth

sometimes acquire a spatulate shape.

Jobbins-Pomeroy (1916) reported Euglenaand Spirogyra were important

food items for black fly larvae but Cameron (1922) found diatoms formed

the main components of the food. Anderson and Dicke (1960) found

diatoms, other algae, and considerable inorganic material in the in-

testinal contents of black fly larvae. Carlsson (1967) listed as larval

food bacteria, plankton, plant and animal parts, pollen and aerial

fallout. Spring flooding leads to large outbreaks of black flies when

rising water temperatures trigger synchronous hatching of many black

fly eggs. The rising stream and river waters make more substrate avail-

able for larval attachment and increase organic debris and hence food

available for the larvae. Bacteria filtered out by S. underhilli led

to speculation of using simuliid larvae as indicators of pollution

(Snoddy and Chipley, 1971). Reisen (1974) found that larvae remove

bacteria and organic and inorganic particulate matter. Young larvae of

Simulium species in nature fed at a faster rate than did older larvae

as indicated by the time necessary to void a plug of dye particles

(Mulla and Lacey, 1976). These authors also found that at a lower

temperature (12.80C 550F) a longer time (35-55 min) was necessary to









eliminate a particulate plug from larval guts than at a higher tempera-

ture (300C = 860F, 20-30 min).

Pupae. Pupae attach to the same substrates as the larvae. The

larvae form silken cocoons in which pupation occurs. Hinton (1958)

mentions a pharate pupa stage where pupal characters are visible within

the last larval skin but the larval mouth parts are still articulated

and feeding continues up to when this "pupa" begins spinning the cocoon.

The cocoons may be thin and rudimentary (Rrinnia), shapeless, irregular

masses of silk (Prosim~utium and some Cnephia), sturdy, coarse cocoons

(Sinuium pictipes) or tapered, slipper-like finely shaped and tightly

woven cocoons (most SimuZium) (Stone and Jamnbackc, 1955). Members of

the Simuliumn subgenus Phosterodoros have cocoons with forward edges that

are convex in profile and have a large opening anteriorly on each side

of the cocoon (Stone and Snoddy, 1969). Field and Law (1961) describe

what Underhill (1944) illustrated for S. jenningsi (as S. nigroparvum),

that is, sexual dimorphism in the cephalic plate of simuliid pupae. The

cephalic plate, a sclerite that begins between the bases of the antennal

sheaths and extends over the cephalic area, is longer and narrower

in the male than it is in the female pupa. The pupae usually face down-

stream and are bordered on each side by tubular filaments which vary in

number and shape according to species and aid in respiration. Cameron

(1922) observed that the number of pupal filaments in S. arcticumt varied

from the usual 12 to 13 or 11 and that one set might vary while the

other group on the same pupa might bear the more typical number of

filaments. Coscaron and Wygodzinsky (1973) mention the variation in

the point of bifurcation or petiole lengths of the respiratory filaments

of different specimens within the same species.









Adults emergence. Tarshis (1968) reported that pupal develop-

ment of three species of Simulium took 1 to 5 days at 20-250C and that

at 100C it took 21 days following pupation before adults began emerging.

Davies et al. (1962) describe how, at emergence, after gas fills the

skin of the submerged pupa, the adult ruptures the pupal exuvium, pulls

itself through a T-shaped opening and quickly floats to the surface in

a bubble of gas (also see Hannay and Bond, 1971, section below). Carlsson

(1967) found that a heavy silt cover on pupae prevented adults from

emerging. Adults may easily be reared from pupae placed on moist filter

paper in petri dishes (Hannay and Bond, 1971; Sutcliffe and McIver,

1974). Wenk (1965) found males emerge sooner from pupae than do females.

Disney (1969) found three species of Simulium in Africa pupate by day

and may prolong pupation to avoid emerging at night. On warm days

eclosion occurred early in the morning, on cooler days eclosion peaks

occurred during the late morning and mid-day and under artificially cold

days emergence was shifted to the late afternoon.

Adults appearance and morphology. Adult black flies rarely ex-

ceed 5 mm in length (Dalmat, 1955). Male and female black flies are

hump-backed in appearance. The males are holoptic, usuallydarkerand

morevelvety than females and often bear a shiny pair of anterior lateral

spots on the scutum (Davies et al., 1962). Costalization (i.e., a

strengthening of the anterior wing veins and a decrease in wing venation

in the posterior portion of the wing), shortening of the legs, evolution

of the calcipala and pedisulcus, increase in head size and shortening of

the abdomen with reduction of tergites and sternites are considered

apomorphic developments (Rohdendorf, 1974; Rubtsov, 1974). Hungerford

(1914) in discussing the anatomy of Simulium vittatumt adults noted that









black fly females possess a single spermatheca. Lewis (1957) presented

morphological information about adults of S. damnnsum and suggestions

on dissection techniques. Bennett (1963b)found the shape and appearance

of the salivary glands of adults valuable in differentiating species.

Hannay and Bond (1971) found raised cylindrical buttons, each with a

wax filament, between the macratrichia on black fly wings and suggest

these structures may aid the adult in keeping its wings dry during

emergence. Sutcliffe and Melver (1974) describedcleaning hairs and

combs of cuticular teeth on the metathoracic legs and cleaning hairs

on the prothoracic legs which are used to clean the wings and head

appendages, respectively.

Adults attraction and trapping. Wirth and Stone (1956) indicate

that male black flies are readily collected at light traps. Snow et al.

(1958) captured adults at light, on vegetation, in cars and biting

mammalian hosts. Fallis and Smith (1964) succeeded in using ether

extracts of birds as attractants for simuliids. Anderson and DeFoliart

(1961) used a variety of caged birds to attract ornithophilic black

flies to traps in Wisconsin. Golini and Davies (1971) found that fe-

males of S. venustum fly upwind to a carbon dioxide source and cease

flying upwind if the CO2 source is turned off. Snoddy and Hays (1966)

and DeFoliart and Morris (1967) made use of the attractancy of CO2! to

capture simuliids. The silhouette of a target rather than the reflec-

tance is more important in attracting black flies (Gillies, 1974).

Peschken and Thorsteinson (1965) state that black flies are more at-

tracted to stationary targets than to moving targets and simuliids show

less discrimination between form and shape of three dimensional objects

than some other hematophagous insects. Bradbury and Bennett (1974a)






-16-


found bloodseeking black flies in the genera Prosimulium, Onephia, and

Simuliwn were more attracted to black, blue, and red than white or

yellow, especially when the colors were of low visible reflectance.

Thorsteinson et al. (1965) describe a tripiod canopy-type Manitoba fly

trap which uses a large black sphere target to make the trap visually

attractive to bloodsucking diptera. Bradbury and Bennett (1974b) found

that black flies could distinguish between targets based on their color,

largely independent of carbon dioxide flow rates. Carlsson (1967) stated

lakes, shoals, bogs and swamps act as "collecting mirrors" for female

black flies seeking ovipositioning spots. Female black flies seeking

oviposition sites oviposited more on green and yellow colored plastic

strips in a stream than on red, purple, white or black strips (Golini

and Davies, 1975). The Malaise trap, a final valuable collecting device

for black flies and other insects,was conceived as a passive or random

flight interception trap;however there is some evidence that the degree

of color contrast between the trap and background vegetation is an

attractance factor (Roberts, 1970 and 1972).

Adult mating. Females of C. de~cotensis have mature eggs at the

end of the pupal stage and copulate with males on damp rocks soon after

emergence. In general, however, simuliids can mate any time from

emergence to oviposition time (Davies and Peterson, 1956). Davies and

Peterson (1956) were unable to induce male black flies to mate by broad-

casting the sound of the female wing beat frequency. Compared to males

which do not form mating swarms, males with mating flights have larger

eyes and enlarged upper facets. Snow et al. (1958) observed that males

of S. pictipes flew upside down with their abdomens curved upward, con-

tacted females as they flew by overhead, settled to a solid substrate,









and copulated venter to venter with the female situated uppermost. The

presence of about one male for every two hundred females of onephia mutata

is considered evidence for parthenogenic reproduction (Davies and Peterson,

1956). Downes (1965) reports Prosimulium .ursinum is a maleless species

in which the females do not even emerge from the pupa but disintegrate

shedding the eggs into the stream.

Adult feeding. Black flies have been collected from flowers

(Davies and Peterson, 1956). Hocking (1953) stated that black flies and

other northern biting flies obtain the energy for flight almost entirely

from floral nectar. Lewis and Domoney (1966) reviewed the importance of

sugar feeding on bloodsucking, autogeny, and parasite development and re-

ported finding glucose, sucrose, fructose and other sugars in the crops

of 101 wild caught Simutium. There are scattered reports of black flies

being attracted to and feeding on cold-blooded animals (Hagen, 1883;

Jobbins-Pomeroy, 1916; Smith, 1969), but of more importance are their

ornithophilic, mammalophilic, and anthropophilic bloodsucking habits.

Onephia dacotensis, Gyrmnopais hotoptious, Tw~innia ti~bbesi and a few

other black fly species acquire sufficient nutrients during the larval

stage to produce eggs and do not suck blood (Davies and Peterson, 1956;

Shewell, 1957). Rohdendorf (1974) suggested that limited larval food

stimulated the adult female black flies to hunt and feed on vertebrates.

Cameron (1922) mentions the tenacity and persistence black flies ex-

hibit when feeding and states that once the mouth parts are securely

inserted into the skin the insect is not easily disturbed. Ussova (1961)

states that the mandibles pierce a host's skin, the maxillae with re-

curved teeth anchor the proboscis in the skin, and alternating actions

of the cibarial and pharyngeal pumps suck the blood into the esophagus.





-18-


James and Harwood (1969) indicate simuliids are telmophages or pool

feeders which lacerate blood vessels with the toothed, transversely

operating mandibles and vertically operating maxillae. Davies and

Peterson (1956) record a wide, natural host range for some species such

as S. venustum duck, crow, heron, deer, and human. Others such as

P. hirtipes (now three species) and S. decorum which feed on? mammals in

the field fed on birds when placed in vials on the avian species in

the lab. Yang and Davies (1974) examined the salivary glands of three

black flies and found an anticoagulant factor which keeps the blood

fluid for movement into the gut. These authors also report an agglutin

factor in flies at least twelve hours old.

Adult egg development and egg laying. Cameron (1922) found

development of the ovaries of female black flies followed a successful

blood meal. Davies and Peterson (1956) describe females of several

species with weak teeth or only hairs on the mandibles and maxillae. These

flies do not suck blood and emerge with already ,mature eggs. Davies

and Peterson (1956) indicate eggs develop five to twenty-one days after

emergence with the longer time involving a prolonged blood meal search

under natural conditions. Immature and mature eggs are found together

in the ovaries which suggests at least two ovarian cycles (Cameron,

1922). Females may survive long enough for three batches of eggs but

few probably live this long in the field (Davies and Peterson, 1956).

Cameron (1922) reported that the eggs of Simutium simile (=S. arctioum)

are oviposited on rocks in large cake-like masses embedded in a soft

gelatinous matrix, Davies and Peterson (1956) mention Prosimulium and

Cnephia species which deposit eggs freely while flying down or across

a stream. Stone and Jamnback (1955) indicate S. vittatum lays strings









of eggs in a gelatinouls matrix. Golini and Davies (1975) in Canada

found that female black flies settled at the water line on trailing

cattail leaves and deposited large (16 cm x 2 em) irregular egg masses

one to five layers deep in a gelatinous substance. Davies and Peterson

(1956) found simuliids oviposited an average of 200 to 500 eggs per

female while Golini and Davies (1975) report an average of 417 eggs

for each female.

Adults life cycle, longevity, range, and general activity.

Tarshis (1968) reports a 21 to 25 day period for mixed cultures of five

Simuliaum species to develop into adults after eggs were placed in aquar-

ia. Raybould and Grunewald (1975) found developmental time from egg to

adult ranged from 18 to 50 days for African black flies.

Dalmat (1955) in Guatemala used colored dyes and the release-

recapture technique and found marked female black flies survived as

long as 85 days and traveled as far as 9.7 miles. Bennett (1963a) tagged

females of S. rugglesi by feeding them on ducks injected with a phos-

phorous-32 solution and recovered labelled flies up to 28 days later and

9.6 km? distant following watercourse paths. West-et al. (1968) exposed

black fly larvae and pupae to 32P-treated water and recovered radioactive

flies as far as 33.5 km (20.8 mi) distant. Bennett and Fallis (1971)

found S. eurycdniniculum flew up to five miles from the release point

and report the average life span of the females was at least two to

three weeks. Hocking (1953) summarized published records of flight

ranges which reached a maximum of 145 km for S. reptans columbaczen~se.

Wellington (1974) reported frenzied activity in black flies which

was apparently correlated with barometric pressure changes as a storm

system approached. Cameron (1922) observed swarms of male black flies





-20-


or males and females on warm, cloudy days with rain threatening or

falling gently. Carlsson (1967) reports that rapid changes in tempera-

ture, air pressure, and light seem to increase the activity of all

simuliid species but he also mentions even a light breeze reduced black

fly activity considerably. Hocking (1953) found flight was continuous

in Simulium species down as low as 12.80C (550F).

Concerning nocturnal behavior Dalmat (1955) observed that black

flies in Guatemala move down to the base of plants as the sun sets and

can be captured at night using a lantern and sheet when the insects

emerge from their resting sites near the ground. Wolfe and Peterson

(1960) describe climbing trees and sweep netting twenty-five feet off the

ground to capture black flies at night in Quebec. A4t dawn Wolfe and

Peterson observed simuliids flying down from the canopy. Recently in

India researchers used light traps and found that black flies were active

throughout the night with a peak of flight activity occurring around

midnight (Datta and Dasgupta, 1974).

Lab colonization. Black flies have been collected in nature in all

stages and have been brought to the lab for experiments. Tarshis (1965a)

describes techniques for collecting and shipping viable black fly eggs.

Tarshis (1965b) and Tarshis and Adkins (1971) discuss collecting

large numbers of black fly larvae on artificial cloth substrates and

mention techniques for transporting larvae in aerated containers.

Tarshis (1971) discusses rearing black fly pupae to adults in individual

brass strainer cloth cylinders connected to aquarium air stone units.

Tarshis (1972, 1973) describes field collection, laboratory rearing of

immatures to adults, and successful feedings of females of C. ornitho-

philia on ducklings in the lab.





-21-


Wenk and Raybould (1972) point out that colonies of insects aid

critical studies on their biology, provide adults free from infection

for transmission work and can provide abundant material for investiga-

tions at times when natural populations are limited. Dunbar (1969)

mentions that hybridization experiments supported by colonies of flies

and sufficient knowledge of mating requirements can provide valuable

information concerning the species status of insect forms. Dalmat (1955)

found carbon dioxide stimulated feeding and oviposition and succeeded in

inducing 40% of 65,000 S. ochraceum, S. callidum,; and S. metaieum to

feed and 20% to oviposit but no eggs developed into larvae. Pield et al.

(1967) found that females of S. vitbatum confined in a vial bit man,

quail, and rabbits. By impaling a male on a minute and brushing the

male genitalia against the genitalia of the female coupling could be

achieved. No female of S. vittatum which mated survived to oviposit

and eggs deposited by other, unmated, females in the artificial flows

available failed to hatch. In the laboratory Wenk (1965) successfully

reared, mated, blood fed and achieved oviposition of viable eggs with

Boophthora ery~throcephala. Wenk and Raybould (1972) working with the

Kibwezi form of S. damnrosum likewise reared, mated, blood fed and ob-

tained viable eggs from female black flies in the lab. Mating, which

was also achieved with a member of the S. bovis complex, occurred in

partially lighted emergence cages and was confirmed by the presence of

sperm or spermatophores. Raybould and Grunewald (1975) review the litera-

ture on lab colonization of black flies and mention difficulties confront-

ing researchers: inducing mating, inducing females to feed on blood suf-

ficiently and consistently, erratic viability or hatchibility of eggs

oviposited in the lab, and removing wastes from immature rearing setups.









Although a few black fly species have been induced to complete every

stage of their life cycle in the lab, they are still not considered to

be successfully colonized.



Damage


Jamnback (1973) describes the black fly bite reaction: a hemor-

rhagic spot which develops into an itching wheal; sensitive individuals

may suffer headache, fever, nausea, glandular enlargement, and bites

around the eyes may cause swelling that results in obscured vision.

Loomis et al. (1975) report black flies cause considerable irritation

and tissue damage to horses' ears, head, neck, and belly,producing

wounds and papules. S. archiewn, C. pecwuarn, and S. columbaczense are

described as major pests of livestock (Jamnback, 1973). Fredeen (1974)

mentions black fly outbreaks during 1944-1947 in Saskatchewan which re-

sulted in the deaths of 1100 farm animals including cows, horses, hogs,

and shorn sheep. Fredeen (1975) reports a S. arcticum outbreak in 1972

along the N'orthern Saskatchewan River that killed at least 18 cattle.

Black flies transmit Leucocytozoon parasites to ducks (Fallis and

Bennett, 1966), trypanosomes to ducks (Desser et al., 1975), and

Leucocytozoon parasites to turkeys (Skidmore, 1931; Noblet et al., 1972)

(see also the Leuc-ocytozoon smithi sections in the Literature Review and

Results and Discussion below). Sudia et al. (1975) mention that epi-

demic VEE virus has been isolated from Simuli~um species in Colombia and

although biologic transmission has not been proven, mechanical trans-

mission by contaminated mouth pprts may be possible for at least 72

hours. Travis et al. (1974) report a finding of vesicular stomatitis

virus in black flies in Colombia. Eastern encephalitis virus has been








isolated from a pool of 100 unengorged S. meridionale which indicates

this species may biologically transmit the disease (Anderson et al.,

1961).

Steelman (1976) reports monetary losses in cattle herds due to

worm nodules caused by simuliid transmitted Onchocerca gutterosa amount

to $500,000 each year in Australia. Onchooerca voloulus,the causative

agent of blinding filariasis in mantis vectored by black flies and has

been reported from Africa, Yemen, Mexico, Guatemala, Venezuela, Colombia,

and Surinamf; indigenous cases may also occur in Brazil (World Health

Organization, 1971; Travis et al., 1974; Raybould and Grunewald, 1975).

i~crofilaria of 0. voloulus previously were observed in the eyes and

skin, however, Anderson et al. (1975a) found microfilaria in the urine,

blood, and sputum of persons treated with diethylcarbamazine. Diurnal

periodicity of 0. volvulus microfilaria in the skin has been shown to

correspond with the peak feeding periods of important black fly vectors

in Guatemala and Africa (Anderson et al., 1975b). Duke et al. (1975)

found the transmission potentials of 0. volvulus along breeding rivers

for S. d'awnosum in Africa so high that no communities could survive there.



Control


Physical control. Impoundage of rivers and the creation of reser-

voirs together with planned periodic cutoffs of discharge water has

discouraged or eliminated black fly breeding along many stretches of

rivers (Snow et al., 1958). Removing debris such as planks, tree limbs,

trailing vegetation and other potential substrates for black fly larvae

can help control s~imuliid population levels in streams (Jamnback, 1973).









Chemical control. Carestia et al. (1974) reported that serial ap-

plications of malathion, DibromR or Dibrom plus a repellent for

adult black fly control were unsatisfactory. High winds, dropping

temperatures and decreasing daylight interfered with adult fly activity

and chemical effectiveness. Rapid reinfestation of adults from areas

just outside the treated zone is another reason why most control efforts

have been aimed at immature rather than adult black flies.

Fairchild and Barreda (1945) observed the effectiveness of DDT as

a black fly larvicide. Davis et al. (1957) discussed early attempts at

evaluating the larvicidal properties of parathion dripped into streams,

dieldrin applied by air, and DDT delivered by various ground techniques

as well as by airplane. Evidence of the persistence of DDT in non-target

organisms and decreased susceptibility of black fly larvae from streams

with a history of DDT treatments stimulated a switch to less persistent

materials (Fredeen et al., 1971; Jamnback and West, 1970). Travis et al.

(1970) in field trough tests found Dibram was outstanding in producing

mortality of S. pictipes larvae at .5 ppm and 1 ppm concentrations and

stated Dibrom, GardonaR and CiodrinR deserved more practical stream

tests.' Jamaback and Frempong-Boadu (1966) found chemical formulations

are most effective which give uniform distribution of the insecticide

in the water and have a specific gravity of slightly less than 1.0 to

keep the toxicant near the surface where most of the black fly larvae

are located. AbateR (20%) in Panasol plus .5% Triton X-161 (.998

specific gravity) applied by aircraft eliminated larvae up to .8 km

(.5 mi) downstream from the treatment point. Pelsue et al. (1970) chose

Abate as a black fly larvicide due to its low mammalian, avian, and fish

toxicity and achieved 100% control of larvae in three locations









with .5 ppm delivered for 60 minutes. Detachment of larvae occurred

within 24 hours and reinfestation was noted in 15 to 60 days. Kissam

et al. (1975) reported 2% Abate Celatom granules delivered at 91 g AI/

.4 ha monthly reduced larval populations t~o zero and the populations

only slowly built back up during the two weeks following each applica-

tion.

Fredeen (1974) mentions that methoxychlor, another black fly larvi-

cide, is minimally toxic to vertebrates and methoxychllor and its

metabolites are not concentrated in fish or other aquatic species. In

Canada in 1969 a 15 minute injection of .2 ppm methoxychlor emulsifiable

concentrate caused the disappearance of 96% of the S. arcticum larvae

32 km downstream. Fredeen (1975) reported that a 7.5 minute injection

of methoxychlor at .6 ppm killed 100% of the older black fly instars 40

and 80 km below the treatment area. Younger black fly instars which are

more susceptible were depleted by 96% at a site 161 km downstream from

the injection. Fredeen et al. (1975) indicate that methoxychlor adsorbs

to suspended solids in the water and may act selectively against the

filter-feeding black fly larvae. Following a methoxychlor application

Wallace et al. (1973) noted increased drift but no eradication of non-

target organisms and Fredeen (1975) found that non-target organisms

repopulated more densely than before the treatment.

Investigations of the effect of insect developmental inhibitors on

black flies indicate that significant reduction (75-100%) of adult

emergence can be attained in the lab with at least three black fly

species with AlcosidR at concentrations between .001 and 1 ppm (McKague

and Wood, 1974; Dove and McKague, 1975). Frormmer et al. (1975) reported

that in field evaluation tests in New York DEET-treated light mesh





-26-


jackets were effective in reducing landings per five minutes from 404

on controls to 1.2 on personnel with treated jackets.

Biological control. Cameron (1922) found that a fish of no food

value called the common sucker fed on S. simile (=S. arcticum) larvae

and pupae. Peterson and Davies (1960) review the predators of black

flies which include adult Empididae and Dolichopodidae, Tendipedidae

larvae, Trichoptera, Formicidae, Odonata -adults and naiads, and spiders.

Sommerman (1962) reported empidid larvae fed on black fly larvae.

Snoddy (1968) found the solitary wasp Oxrybelus emarginatum to be a

predator of adult black flies. Peterson and Davies (1960) and Chutter

(1972) report cannibalism among simuliid larvae. Carlsson (1967) in-

dicates predators are unlikely to serve well as man-manipulated biologi-

cal control agents though the tricopteran genera Hiydropsyche and

R~hyacophila might give good results.

One of the earliest accounts of parasitism of black flies is that

.of Strickland (1911) who reported a mermithid nematode that retarded

the development of the larval histoblasts and a sporozoan (microspori-

dian) which caused distorted and swollen larvae. Jamnbackc (1973) states

that eighteen microsporidia have been described from black flies.

Spores are ingested by the larvae, sporoplasm invades suitable host

cells which are often in the fat bodies, multiplication and the pro-

duction of many spores occurs, and the host larvae commonly die. Black

fly pupae and adults have been found infected and transovarian trans-

mission is common. Jamnback (1973) also mentions a fungus Coeiomycidiwn

simulii which occurs in black fly larvae and is usually fatal.

Davies (1958) reports microsporidians, mites, and mermithid nema-

todes as parasites of Canadian black flies and mentions that mermithids









are found in larvae, pupae and both sexes of the adults. From 15 to

60% of the females in emergence collections were found to be infected

with mermithids and females in oviposition swarms were also found with

mermithids. The infected females which attempt to oviposit may serve to

disperse the mermithids and introduce them to additional stream environ-

ments. Welch (1964) indicated that the mermithid life cycle began with

the consumption by black fly larvae of the mermithid, usually in the

infective first juvenile stage. Molloy and Jamnback (1975) observed

direct penetration of the black fly larval cuticle by preparasitic

juveniles of Neomesomermis fluiminais, a mermithid which infects at

least fourteen species of black flies in three genera. Exit of the

parasite from the host is almost immediately fatal to the black fly.

Anderson and DeFoliart (1962) report 49-93% parasitism by Isomermis and

Gastromermis in one black fly species in Wisconsin. As a step toward

the mass rearing of these biological control agents Bailey et al. (1974)

discuss techniques for the mass collection of mermithid postparasites

from field collected black fly larvae.

Batson et al. (1976) reportedatiridescent virus from black fly

larvae in Wales but could associate no major fluctuations in the black

fly population with the presence of the infected larvae.



Florida Ecological Habitats


Florida is a peninsula that lies between 80 and 88 degrees west

longitude and in the same latitude belt as the Sahara, Arabian, and other

large deserts. Exclusive of the Keys, the State extends roughly 644 km

(400 mi) north and south along the peninsula and about the same distance

east to west along the north coast of the Gulf of Mexico. Florida covers





-28-


151,710 sq km (58,560 sq mi)'. The highest point in the State is 105 m

(345 ft) above sea level, at Lakewood in Walton County. From Orlando

south to Sebring another high section occurs and Iron Mountain near

Lake Wales is located about 91.5 m (300 ft) above sea level (Morris,

1973). The earliest geological horizon is the Ocala limestone formed

during the Eocene, 58 million years ago. Modern Florida made its first

appearance during the Oligocene, 36 million years ago, as a small island

about where the counties of Suwanee, Columbia, and Alachua are now

located and then rose as a peninsula during the Miocene (Byers, 1930).

There are three schemes which have been used to break Florida up

into sections according to: 1) vegetation; 2) geology; and 3) edaphic

factors. These schemes are presented to help illustrate the ecological

diversity in Florida, which an the one hand provides a range of habitats

for simulfid species and on the other hand restricts black fly breeding

to certain more suitable regions of the state. Byers (1930) divided

Florida into seven biotic areas based on dominant types of vegetation:

a tropical hammock strip from St. Lucie County through Dade County along

the east coast extending a few miles inland with cabbage palms, mahogany,

ironwood, papaya, epiphytic bromeliads, plus other plants; a grass swamp

area south and east of Lake Okeechabee; magnolia and temperate hammocks,

primarily along the east coast from Flagler County to Indian River

County and on the Gulf Coast from Wakulla through Hernando Counties -

these occur on well drained but moisture holding soils with magnolia,

holly, and red bay as dominant vegetation on the east coast and beech,

elm, sweetgum, hickory, and oaks dominant in the west coast hammocks;

a section of southeastern deciduous forest extending down into Leon,

Liberty and Jefferson Counties; tree swamps in Collier, Columbia, Baker









and Nassau Counties which include Big Cypress Swamp and other cypress

and also black gum swamps; pine flatwoods along the Gulf west of

Wakulla County, on the west coast of the peninsula from Pasco to

Collier Counties, and in the Clay County and Putnam County region -

these flatwoods occur on level, poorly drained land underlain by hard

pan which results in an acid soil upon which grow palmetto, grass and

pines; and, lastly, the southeast coniferous forest with long leaf,

yellow, and slash pines and saw palmetto on well drained uplands.

Cooke(1939) divided Florida into five physiographic areas: central

highlands, Tallahassee hills, western highlands, Marianna lowlands and

coastal lowlands. The central highlands, in the center of the peninsula

from Baker County and Columbia County south to Lake Okeechobee, contain

in their southern section thousands of lakes which in the north section

are accompanied by numerous streams. The Tallahassee hills and western

highlands are composed mainly of red clay with a relatively good number

of streams. The coastal lowlands usually lie less than 30 m (100 ft)

above sea level and include swampy areas such as the Florida Everglades

and marshy areas along the east and west coasts of the state. The

Marianna lowlands in Wlalton, Holmes, Washington and Jackson Counties

contain few permanent flows and consist mainly of swamps, flatwoods,

and sandy hills bearing pines.

Davis (1967) and Smith et al. (1967) present six major land resource

areas for Florida: a southern coastal plain; gulf coast flatwoods;

central Florida ridge; Atlantic coast flatwoods; southern Florida flat-

woods; and Everglades and associated areas. The southern coastal plain,

occupying the northern half of the state from Escambia County to Madison

County,is covered by forests of mixed hardwoods and pines on the lower





-30-


areas and forests of long leaf pines and oaks on upland clay or well

drained upland sand. The gulf coast flatwoods--a strip along the gulf

side of the panhandle from Escambia County through Citrus County, the

Atlantic coast flatwoods primarily in Columbia, Baker, Nassau andBrad-

ford Counties in the north, and the south Florida flatwoods occupying

most of the southern section of the peninsula outside of the Everglades

consist of pine flatwoods and swamp forests (pines, palmetto, herbs,

bay tree, laurel, gum, cypress) on poorly to very poorly drained and

marshy soils. The central Florida ridge is located on well drained

soil in the middle of the peninsula and includes forests of long leaf

pine and xerophytic turkey oak (now mainly planted in citrus) and hard-

wood forests of mixed evergreen and deciduous hardwoods on rich upland

soils. The Everglades are primarily sawgrass, M~ariscus jamaioensis, on

peat and muck soils.

Average January temperatures range from 10-130C (50-550F) in the

panhandle to 18-210C (65-700F) in the Everglades area. Average July

temperatures fall between 27-290C (80-840F) throughout the state. An-

nual rainfall averages 134.62 cm (53 in) (Raisz, 1964). June through

September or October is considered the rainy season in Florida (Byers,

1930) however Berner (1950) indicates that in the northwestern area

rainfall is more evenly distributed throughout the year. There are

seventeen first magnitude springs in Florida (Morris, 1973). Despite

the heavy precipitation there are relatively few surface flows since

much of the water moves in underground channels in the underlying lime-

stone (Raisz, 1964). The vast majority of true streams and rivers are

found north of L~ake Okeechobee. Morris (1973) indicates there are 1,711

streams, rivers, and creeks in Florida with a total length of 16,989 km

(10,550 mi).








Berner (1950) states that in Florida there are relatively few

intermittent streams. He divides permanently flowing streams in the

State into: sand-bottom creeks with loose shifting sand and little

vegetation; sand-bottom creeks with fairly loose sand and considerable

vegetation; silt-bottomed creeks with little vegetation; and silt-

bottomed creeks with considerable vegetation. Stagnant rivers occur in

south Florida where they serve primarily as drainage canals for the

Everglades and though there is considerable vegetation along the shores

of the rivers their fifteen to twenty-foot depths restrict plant growth

toward the middle. Larger calcareous streams contain water which rises

from springs, are basic in pH reaction, and in shallower sections are

lined on the bottom with Vazllisneria (eel grass), Sagittaria (arrowhead),

algae and other plants. Erving (1971) notes that the temperature for

Florida springs is about 220C (720F) year round. While noting springs

are alkaline Berner (1950) also indicates that marsh and swamp water can

be very acidic reaching a pH of 3.6 or below. He further adds slow

flowing rivers to his classification of Florida's streams and rivers

and included here? are the Suwanee, Apalachicola and other rivers which

have extensive drainage areas. Wood and Fernald (1974) list twenty-

eight major drainage basins and streams in Florida. The two largest,

the Apalachicola River Basin and the Suwanee River Basin drain 50,777

sq km (19,600 sq mi) and 25,984 sq km (10,030 sq mi), respectively,

some of which lies outside of Florida. Wood and Fernald (1974) indicate

that peak annual flows occur in eastern rivers in September and October

with buildups since June while more western rivers (Chipola, Apalachi-

cola) reach maximum flow during March and April. In northwesternl

Florida a continental weather pattern predominates whereas a more









tropical weather routine predominates in central and south Florida.

Beck (1965) recognized five stream types in Florida: sand-bottomed,

calcareous, swamp and bog, larger rivers, and canals. He points out

that due to velocity, substrate, and spring discharges many of these

types of flows can be recognized at different points along a single

watercourse and cites the Suwanee River which is successively a swamp

and bog stream, a sand-bottomed stream, a calcareous stream and a larger

river of mixed origin. Beck (1965) classifies swift flow in Florida's

streams as that velocity suitable for Plecopterawhile simuliid popula-

tions indicate conditions of moderate velocity. Below these two levels

the speed is considered low. A general progression is recognized with

the swifter portions of upper rivers marked by eroding limestone and

thick growths of moss, midpoints along rivers being sand-bottomed and

lower, slower portions of rivers containing deposits of finer materials

on the bottom. Beck (1965) lists the sand-bottomed stream as the most

common type of stream in Florida. The fauna consists of Hydropsychid

and Philopotamid caddistlies, simuliid larvae, Ple-coptera, Stenonema

mayflies, orthocladine chironomids, and Corydatis. In these sand-

bottomed streams the pH reaction is usually between 5.7 and 7.4 and

they display moderate to high color and moderate to swift velocity. The

bottoms consist of sand, leaf deposits, and limestone outeroppings, and

plant growth may be dense. Beck mentions that swamp and bog streams are

highly acidic (pH reaction 3.8-6.5), sluggish flows found in the coastal

lowlands and central highlands with origins in swamps and marshes.

Calcareous streams are cool, clear flows of spring origin with dense

growths of vegetation. These flows are alkaline (7.0-8.2 in pH), bear

large mollusc populations, are generally of low color, of variable










velocity and have sand, clay or limestone bottoms. The flows in the

larger river category usually carry considerable silt and are turbid,

have high and steep banks and bottoms of coarse sand and limestone.

They demonstrate a pH reaction of 6.5-7.4 and have few shallow places

and few aquatic plants. Beck (1965) mentions from about the area of

the St. Lucie Canal to south of Homestead no natural streams of any

consequence remain along the east coast of Florida and, instead, canals

are prevalent although in the past the Miami River had a swift flow and

rapids (Fairchild, 1976 personal communication).

The above discussion provides an introduction to the climatic,

edaphic, biotic and hydrologic conditions which are found in the State

of Florida.



Leucooptozoon smith


During the 1880's Danilewsky observed parasites which were later

described and designated as species of Leucocytozoon and Haemoproteus

(Bennett et al., 1965). Hsu et al. (1973) list 67 species of

Leucocytozoon, one of which is reported from Meteagris gallopavo, the

domestic and wild turkey. Theobald Smith in 1895 first reported a

protozoan-parasite in the blood of turkeys in Massachusetts and Rhode

Island (Smith, 1895). Similar gametocytes were observed in the blood

of turkeys in France and named Haemamoeba smith (Laveran and Lucet,

1905). The following taxonomic scheme which shows the position of the

turkey parasite now known as Leucocytozoon smith was modified from

Aikawa and Sterling (1974) by crediting Sambon (1908) not Danilewsky

(according to Bennett et al., 1975) with the genus Leucocytozoon:








Phylum Protozoa Goldfuss, 1818, emend. von Siebold, 1845
Subphylum Sporozoa Leuckart, 1879
Class Telosporea, Schaudinn, 1900
Subclass Coccidia Leuckart, 1879
Order Eucoccida Leger and Duboscq, 1910
Suborder Haemosporina Danilewsky, 188i5
Family Leucocytozooidae Fallis and Bennett, 1961
Genus L~eucocytozoon Sambon, 1908
Species smith (Laveran and Lucet, 1905).


The following authors observed L. smith in turkeys in their respec-

tive states or countries: Atchley (1951), Bierer (1954), Jones and

Ricbey (1956) and W~ehr (1962) in South Carolina; Savage and Isa (1945)

and Bennett et al. (1965) in Canada; Volkmar (1929) in Minnesota and

North Dakota; Hinshaw and McNeil (1943) in California; Johnson (1945) in

Michigan; Kozicky (1948) in Pennsylvania; Skidmore (1931) in Nebraska;

Stoddard et al. (1952) in Georgia; and Byrd (1959) in Virginia. Volkmar

(1929) also lists L. amithi from Germany and the Crimean Peninsula and

Cook (1971) lists Asia. Travis et al. (1939) reported Leucocytoaoon

smith from wild and domestic turkeys in Florida as well as Georgia,

Missouri, Alabama and South Carolina. Simpson et al. (1956) and Forrester

et al. (1974) also reported Leu~cocytozoon smithi from Florida turkeys.

Solis (1973) exposed turkeys, ringnecked pheasants, chickens, bob'white

quail, pekin ducks and chukar partridges in an area where L~. snrithi

occurred and found that only turkeys were susceptible to L. smithi.

Fallis et al. (1974) and Aikawa and Sterling (1974) present in-

formation an the life cycle and structure of the intracellular parasite,

L. smith. Leucacotozoon belongs to the same suborder, Haemosporina,

as Plasmodiun and Hiaemoproteus and undergoes a similar life cycle.

Unllike PLasmodium, however, there is no eryrthrocytic schizogony and in

addition to the red blood cells being invaded by merozoites as in

Haemoproteus (and Plrsmrodiuw)lin L~aucocytozoon white blood cells are









also used as sites for gametogony (Huff, 1942; Fallis et al., 1974).

Gametocytes, in the case of L. smith, grow and split the host nucleus

in two (Sambon, 1908; Volkmar, 1929). Plasmodium species are tran~s-

mitted by mosquitoes and Haemnoproteus (Parahaemoproteus) species by the

Hippoboscidae and Ceratopogonidae (Fallis and Bennett, 1961a and b;

Bennett et al., 1974). Leucocytozoon, is known at present to be trans-

mitted only by black flies. A parasite of chickens which is vectored by

Culicoides was designated Akiba caulleryi by Bennett et al. (1965) but

Akiba has recently been considered as a subgenus of Leucocytozoon (Hsu

et al., 1973; Fallis et al., 1974). Gametocytes are ingested with the

blood of an infected turkey by a feeding black fly. Roller and Desser

(1973) observed diurnal periodicity with L. simondi where peak gameto-

cytemia corresponded with peak activity and biting periods of the vector.

It has been suggested that there is periodicity of the gametocytes of

L. smith in the peripheral blood and correlation with the periodicity

and feeding peaks of the vectors (Moore et al., 1974). Early in the

infection each gametocyte appears as a round body in the parasitized

cells; later the gametocytes deform each blood cell into the charac-

teristic spindle shape (Huff, 1942). Desser et al. (1970) found with

L. sim~ondi elongated cells resulted when merozoites developed in leuco-

cytes and round cells resulted from invasion of red blood cells. Macro-

gametacytes stain more darkly with Glemsa stain than microgametocytes.

Within minutes of ingestion the microgametocytes and macrogametocytes

escape from their host blood cells. The microgametocytes exflagellate

and syngamy occurs with the macrogametes. The zygotes formed grow into

elongate ookinetes within twelve hours after the initial blood ingestion.

Sections of flies have revealed ookinetes in the process of penetrating








the midgut of the fly (Fallis et al., 1974). Fallis and Bennett (1961a)

working with L. simonzdi detected only sluggish movement of ookinetes

and expressed doubt that this motion could facilitate penetration of

the gut wall of a black fly. Howard (1962) working with Plasmodium

gallinaceum1 observed no mobility or active penetration by zygotes.

Howard suggests a largely passive incorporation into the gut wall to

within 5 microns of the external basement membrane as the blood meal is

digested and the distended gut returns from a squamous cell configura-

tion to its original columnar cell morphology. Desser (1970) described

a pore, strengthening struts, microtubules and elongate convoluted

micronemes in the apical cap of the anterior end of L. simondi cokinetes

and suggested these structures might aid penetration through the peri-

trophic membrane and into the fly midgut epithelium. In as little as

48 hours the ookinetes develop into oocysts containing 50-100 sporozoites

just beneath the outer membrane of the gut. Sporozoites escape from the

oocysts and penetrate and collect in the salivary glands. Desser (1970)

describes structures on the anterior end of sporozoites of L. simondi

which may contain proteolytic enzymes and aid in entering the salivary

glands and, later, host liver cells. The sporozoites pass out the

proboscis of the black fly with the salivary fluids when the fly bites

another host. The sporozoites of L. sm~ithi invade the liver parenchymal

cells of the turkey and grow into hepatic schizonts. Huff (1942) re-

ports with other species of Leucocytozoon megaloschizonts in the heart,

spleen and other host tissues. Such schizonts are not normally observed

with L. smithi; however Siccardi et al. (1974) reported finding magalo-

schizonts in the kidneys of infected turkeys fed coccidiostat medication.

The schizonts by asexual multiplication (schizogony) produce merozoites





-37-


which enter red and white blood cells. Peters (1971) suggests that in

the related genus Plasmodium merozoite penetration may involve proteolytic

enzymes and be active or may be more passive with the merozoites being

engulfed or invaginated into the blood cells. The merozoites grow into

gametocytes in the blood cells and the cycle is completed. The prepatent

period or time elapsed between entrance of the sporozoites and appear-

ance of gametocytes in the peripheral blood is 10 to 16 days.

Symptoms associated with leucocytozoonosis include: loss of

appetite, emaciation, wheezing breathing, congested heart and lungs,

drooling, drooping wings, sitting on the hocks, enlarged liver and

spleen, anemia, impaired imrmunalogical responsiveness, and death (Wehr,

1962; Salsbury, 1971; Siccardi et al., 1974). Turkeys under 12 weeks

of age are often severely affected but mortality in older birds has

also been reported (Simpson et al., 1956). Many birds with high para-

sitemias appear outwardly normal. Birds that do not die may continue

to carry the parasite for as long as 14 years (Skidmore, 1931). Borg

(1953), Simpson et al. (1956) and others suggest that the pathogenicity

of L. smith has not been proven conclusively and that L. smith may be

an additive debilitating factor which when combined with other factors

such as blackhead, fowl cholera or leukosis results in bird mortality.

Stoddard at al. (1952), Skidmore (1931), Savage and Isa (1945) and

others however have observed flock mortality up to 75% where bacterial

cultures and other tests revealed L. smith as the sole disease organ-

ism present. Jones and Richey (1956) indicated annual death losses due

to Leucocytozoon in one county in South Carolina averaged 5%. In

1973,132 million turkeys were raised in the U.S. and farmers grossed

$934 million (Poultry Digest, April 1974; Agricultural Situation,








November 1974). In the absence of severe threats by disease this indus-

try will continue to grow as the public's demand for poultry and its

products like turkey rolls and t.v. dinners as an alternative to beef

increases.

A number of black flies have been incriminated as possible vectors

of L. smith. Skidmore (1931) incriminated S. occidentale (=meridionale)

as a vector of L. smith in Nebraska by grinding up a number of f-lies

that had fed on infected turkeys, injecting them in saline into clean

birds, and 12 days later observing gametocytes of L. smith in the tur-

keys' blood streams. Johnson et al. (1938) in Virginia exposed uninfected

turkeys to the bites of S. nigroparvum (=jenningsi) and obtained

L. amithi transmission. Underhill (1944) further substantiated this

work but Byrd (1959) was unable to infect clean turkeys by macerating

and injecting S. jenningsi that had fed on diseased birds. Wehr (1953)

by injection and Jones and Richey (1956) by fly-bite incriminated

S. skossonae as a vector of L. smith. Byrd (1959) succeeded in

infecting turkeys with L. smith by grinding and injecting females of

Prosimulium hirtipes that had fed on infected birds. I have seen no

further follow up of this work and Fallis et al.(1974) do not list any

member of the former P. hirti~pes complex as a vector of L. s ithi.

Noblet et al. (1972) ground up in saline and injected infected females

of S. congareoenruAm into turkeys and incriminated this species as a

suitable or possible vector for L. smith. Savage and Isa (1945) and

Anthony and Richey (1958) have reported leucocytozoonosis in the sup-

posed absence of simuliids although other biting flies such as CulcZcoidesJ,

Stomorys, Di~achlorus and mosquitoes were present.

Control of leucocytozoonosis can be achieved by locating :urkey

flocks at least 12.8 km (8 mi) from known breeding locations of potential









vectors and in areas free from possible wild turkey disease reservoirs.

Control of black flies by large scale aerial treatment of streams with

larvicides has been shown to markedly decrease the level of

parasitemias of L. emithi in sentinel turkeys (Kissam et al., 1975).

Fine mesh wire screen enclosures might be a valuable preventive tech-

nique with smaller flocks. Clopidol, a coccidiostat, at .025 and

.0125% in feed is effective in reducing the number of L. smithi para-

sites in turkeys as indicated by blood smears (Siccardi et al., 1974).

Investigators of leucocytozoonosis in turkeys in Florida have

studied the presence, consequence, and distribution of L. smithi in

the State but have not proven specific vectors. Travis (1939) reported

that nearly 100% of the wild turkeys in low areas of Georgia and Florida

were infected with L. smithi and suggested that an aquatic breeding

insect might be involved. Simpson et al. (1956) investigating out-

breaks of L. smith in turkey flocks near Palatka, Florida, reported

finding S. slossonae and a species #158 (=S. jonesi Stone and Snoddy,

1969) breeding near the turkey flocks and suggested that S. slossonae

might be an important vector. Davis et al. (1957) reported S. slossonae

as prevalent in Florida. Forrester et al. (1974) found a 72-75% in-

cidence of L. smith in 484 turkeys more than one month old sampled

from 10 localities in Florida. These authors also noted that a drop in

the infection rate of L. smithzi observed in wild turkeys in the Sikes

Fisheating Creek Wildlife Management Area and other areas of the State

in 1971 corresponded with low rainfall and low stream conditions and

theorized that reduced numbers of potential black fly vectors may have

resulted in the reduced prevalence of Leucocytozoon in turkeys.














CHAPTER III
MATERIALS AND METHODS



Black Fly Survey


Primarily, efforts in this survey concentrated on collecting larvae

and pupae from Florida's streams and rivers. In Alachua County ten

streams were selected and in nearby counties an additional twenty flows

were chosen which were fairly permanent and represented a diversity of

ecological conditions. An attempt was made to secure samples from each

of these streams at least once a month. Approximately the same location

was revisited each time to obtain some consistency in stream and sub-

strate conditions in order to judge seasonal replacement phenomena and

normal population changes. Other sites in south, southwest and west

Florida were chosen, generally along highways or country roads for ac-

cessibility, which were visited at least quarterly to obtain records at

all seasons of the year.

At each location specimens were removed with forceps or placed

still attached to small portions of grass, twigs and so on into four-dram

lip vials with neoprene stoppers. Each vial contained 80% ethyl alcohol

and a code number penciled on a small slip of paper. The immatures,

collected in 30 to 45 minutes were deemed sufficient to indicate numbers

and species present. Dark pupae, indicating an advanced state of develop-

ment, were placed in four-dram screw cap vials on a strip of paper toweling

moistened in the stream and were held alive in order to allow the adults

to emerge. Images could be reared very successfully in this manner from





-41-


older and frequently, even younger, more pale pupae. The forceps used

were secured to my wrist by a string to prevent their loss when my

balance was upset by the current, when I was wading and saw an occasional

water moccasin, and at other critical times. A 1.53 m long rake and a

modified, extendible, golf ball retriever were used to obtain substrate

samples and specimens where it was impossible to wade.

At each collection location stream dimensions, velocity, pH, tem-

perature, color, and substrate were recorded an special data cards along

with indications of the attachment substrates of the immatures and an

estimate of the population size. Temperatures were obtained with a

metal-cased pocket thermometer, range -lo to 490C (300 to 1200F). Initial

attempts were made to determine the pH of the streams using two portable,

electronic pH meters. After obtaining erratic, inconsistent results in

the field with the meters, pH papers (pHydrion papers Micro Essential

Labs, New York) were tried with more success. These pH papers come in

ranges such as 3.5-5.5 and 6.0-8.0 between I and 12 pH units with illus-

trated color differences at .5 pH unit intervals and are rated accurate

to .25 pH unit in buffered solutions. The papers were checked regularly

for accuracy with pH 4.0, 6.5, 7.0 and 10.0 buffered solutions. Very

acidic readings were confirmed by bringing chilled water samples into

the lab and checking them on a Beckman pH meter. Experiments with

electronic, propelleredGurley flow meters proved them too cumbersome to

set up and difficult to use in the streams which were often shallow and

clogged with submerged vegetation. The floating cork technique suggested

by Dalmat (1955) and others was used. A weighted cork 3.8 cm in diameter

which floated low in the water and was connected to a 3.05 m (10 ft)

long string was timed with a stop watch to determine the stream velocity









at different points where larvae and pupae were located. Most immature

black flies were encountered on trailing vegetation at or near the sur-

face and thus the floating cork velocity figures should reasonably

reflect immature habitat conditions. A ruler one meter long with

centimeter markings was used to determine stream depths and widths. The

widths of larger flows were estimated by pacing across them, when possi-

ble, or by pacing across a nearby bridge. Other omnipresent items on

collecting trips included an insect net an-d a snake bite kit.

Alcohol specimens and pupal vials from each site were placed with

the data card into cloth bags and transported to the lab where identi-

fications were made with the aid of a Bausch and Lomb stereamicroscope.

Larvae were usually identifiable without dissection (except unraveling

of the respiratory histablast); however representatives of each species

were dissected and mandibles, respiratory histoblasts, cephalic apotome,

gular notch, antennae and fans, and anal sclerite and hooks were pre-

pared in cellosolve and mounted in balsam under individual small cover

slips. Adults which emerged were allowed to harden for a few hours and

were then killed and placed in alcohol, prepared and mounted in balsam

on slides, or pinned on minutin nadeln inserted in white polyporous

pieces on #3 insect pins.

In addition to immatures, some adult collecting was undertaken

using a variety of traps. Black fly adults have been obtained in Florida

by other individuals using light traps and Malaise (flight) traps. In

this research three types of adult fly traps were used: the Manito'oa

trap (Thorsteinson et al., 1965), the blackout box trap (Anderson and

Dicke, 1960), and a modified ramp-type trap. The latter two types of

traps will be discussed with the Leucocytozoon. work. A modified Manitoba









trap (Fig. 1) was constructed using 3 bamboo poles, each 2.1 m (7 ft)

long, with a hole drilled vertically into the uppermost node of each

pole into which a leg of a metal tripod laboratory ring stand was in-

serted. A triangular serex or organza canopy 1.2 m (4 ft) tall was

positioned inside the leg frame and anchored at the three lower corners

by strings tied through a hole in each pole. The apex of the tapering

canopy with a 5.1 cm (2 in) wide elastic opening was drawn up a short

distance through the ring opening and stretched around the lower lip of

a 17.8 cm (7 in) tall lantern jar, the collecting vessel, which rested

on the ring. Th-e jar was topped with a double layer of white serex or

organza held in place with rubber bands. The jar contained an inverted

plastic funnel affixed to the jar with hot plastic glue. On strings

attached around the base of the jar and hanging into the canopy was

suspended a 1.2 m (4 ft) long cord with a black, cylindrical (20 cm

wide, 22 cm tall) metal can or a black cardboard triangle, 63 cm on

each side, on the end. A 23 cm wide black plastic "skirt" was sometimes

fitted over the top of the canopy and positioned at the lower margin of

the canopy to accentuate the "window" effect. A 31 cm long cloth bag

which contained 1.36-1.8 kg of dry ice was also hung inside the canopy.

The dry ice and the black target attracted Simuliidae and other Diptera

which flew or walked up the canopy into the collecting jar. Adults

could be aspirated from the jar by removing the outer cloth layer and

inserting an aspirator tube through a small slit in the lower layer of

material. Specimens could also be taken to the lab in the glass jar and

immobilized by cold in a refrigerator, though condensation inside the

jar often wet the specimens. Manitoba. trapping was accomplished using

one or two traps at a time in six counties at twelve locations for one





-44-


C'? -

'~.., $~
~~~~
r~b-i~~a a


Figure 1. A modified Manitoba trap with a black plastic skirt.









to eight hour periods during the months of January through October. The

air temperature, wind speed and relative humidity were measured during

the collecting periods. Trapped adults were pinned or mounted on

slides as with reared adults. Identification of irmmatures and adults

was possible using taxonomic keys in Stone and Snoddy (1969), Stone

(1964) and smaller publications on individual species.



Leucoc~ytozoon amithi Transmission


In the investigation of the transmission of L. smith to turkeys

in Florida, modifications of Koch's postulates were used as guidelines.

The first aim was to establish an association in nature between the

host, the disease, and the vector. It was necessary to have a sus-

pected vector, preferably a clean, reared one, feed on an infected host

in the lab and to observe the stages of the parasite, especially the

infective stage, the sporozoite, in the fly. A clean host was then to

'be infected by a vector which had become infected in the lab with the

Leucocytozoon parasite being recovered in the gametocyte stage from the

formerly uninfected turkey.

In order to confirm the reported presence of L. smithi in certain

areas such as the Lochloosa Creek and Fisheating Creek Wildlife Manage-

ment areas (L. Williams, 1974 personal communication; Forrester et al.,

1974), I set out sentinel turkeys. The same was done to establish the

presence of the disease in other areas, and to obtain infected hosts to

serve as donors for transmission studies. These turkeys, like all clean

poults used in the transmission studies, were either hatched from fertile

eggs in a fly-proof room or obtained as disease-free one-day old poults

and held in a fly-proof room until use. Difficulties in obtaining clean










poults for experiments early in 1975 when winter and early spring species,

suspected to be L. smithi vectors, were flying were overcome for work

in early 1976. Usually three birds were placed out in the field near

a black fly stream in a cage for about a week and provided with food

and water (Fig. 2). There were three primary sentinel sites, all in

Alachua Co., and additional birds were also set out for short periods

at Fisheating Creek, Glades Co. (see Table 5). Sentinels were also

placed out on a few occasions in a ramp trap described below. In addi-

tion I worked with Dr. D.F. Forrester at Fisheating Creek during 1974

when he exposed many young turkeys for two week periods in cages in

cypress trees and on the ground and in 1975 when cages and ramp traps

were used for turkey exposures.

To determine the species of black flies generally present at the

sentinel sites, immature stages were collected and adults on the wing

were sampled with Manitoba traps as described in the previous section.

To identify black flies specifically attracted to turkeys I used blackout

box traps (Fig. 3), ramp traps (Fig. 4) and exposed turkeys (Fig. 5).

Anderson and DeFoliart (1961) used the technique of blackout box trapping

in Wisconsin. In Florida the blackout box traps were used at two

locations in 1974 and an additional seven sites during 1975 (see Table 7).

A turkey was exposed in a cage on the ground or on a platform in a tree

for about 15 minutes. A large cardboard box with two plastic collecting

cups on the top which appeared as bright areas inside the otherwise dark

box was then placed over the cage and bird. Black flies completing their

blood meals or unfed but on the turkey exhibited a positive phototactic

response, flew to the collecting cups and were captured. The flies were

removed to holding cartons, the turkey was re-exposed, and the process

was repeated.





-47-


;.j13 '' ~k9- );~x~i~


Figure 2. Sentinel turkeys in an exposure cage.


Figure 3. A blackout box trap in the field.





-48-


Figure 4. One view of a ramp trap.


Figure 5. An exposed turkey in the field.


uirPLP)iC' n






t I (1~

1]L~





-49-


An omnidirectional ramp trap was used with the hopes that black

flies would be captured without the constant presence of the collector.

The trap was used at three locations from April to July 1975 (see Table

6). The ramp trap was a wooden-framed cube approximately .92 m (3 ft)

in each demension with 4 slanted lower level ingress panels of aluminum

framing and organza material and 4 upper, vertical organza-paneled

windows. A cage with host animals was placed and suspended inside the

trap through a hinged access opening cut into the solid plywood top.

The attracted insects would fly or bounce up the slanted ingress open-

ings, enter the trap to feed on the hosts, theoretically be unable to

escape and later be aspirated from the inside of the bright vertical

cloth panels or windows.

By capturing flies off normally one or two exposed turkeys it was

possible to tell which species approached the birds and, more signifi-

cantly, to observe which species fed on the birds. Capturing flies off

exposed turkeys first involved immobilizing the host. Masking tape

was wrapped around the turkey's legs just above the feet which were

thus held together. The bird was placed on its side and a layer of

gauze followed by a strip of wide masking tape was placed across the

turkey's neck, chest, and legs and affixed to the platform which was

usually a large plastic tray. The upper wing was propped up to provide

the black flies with easy access to bare skin. Using this technique it

was possible to monitor the number and type of ornithophilic adults

present and capture them as well as observe their feeding locations,

duration and behavior. Their feeding could also be assisted and securing

of blood-fed flies from exposed infected turkeys could be more readily

ensured. To do this, flies that landed on the feathers of the wing or





-50-


elsewhere were quickly aspirated before they could scurry beneath the

feathers and were placed in the glass aspirator tube against the bare

skin of the underside of the wing elbow, the bare skin of the chest

over the rib cage or against the turkey's neck and allowed to feed to

repletion. The fly was observed, the feeding timed, and the turkey

soothed and kept as still as possible to assure a complete blood meal.

Once fed, the flies were transferred to paper pint ice cream cartons with

netting tops, provided a moistened paper towel to maintain high humidity

and transported to the lab. The exposed turkey technique was used to

collect black flies on numerous occasions from January through August

at 8 locations in 3 counties (Table 8).

To obtain black flies that had not been exposed to infections in

the field and to obtain specimens of known identity, especially when

working with species in the Simulium subgenus Phosterodoros which are

difficult to distinguish as adults and are much easier to separate as

pupae, adults were reared in the lab. Some adults were reared in pupal

vials from field collected pupae and other adults were reared form

field collected larvae and pupae in an aquarium with aerated water and

a fine mesh netting cover.

Black flies which fed on exposed infected hosts in the field were

held in the lab for at least two days after feeding and then allowed to

bite an uninfected bird. All adult flies maintained in the lab were

held in paper pint-sized ice cream cartons covered with netting and

provided with moist cotton for water and a raisin for nutrition. Up to

four cartons were kept at one time together in either a large plastic-

covered aquarium or a glass desiccator-type container (Fig. 6) where

high humidity was maintained by including a sponge half immersed in a



















































Figure 6. Glass container and paper cartons used for holding black
fly adults alive in the laboratory.









cup of water. Flies captured in an unfed condition in the field or

reared in the lab were allowed to feed on an infected turkey, held for

two to seven days and then allowed to feed on a clean bird. All

laboratory feedings involving uninfected hosts were conducted in a fly-

proofed former bull room at the Veterinary Science area at the University

of Plorida. This room was incandescent lighted,had a double door entrance

and had windows covered with very fine mesh organza material. Feedings

on infected hosts were conducted either in the Veterinary Science room

or at the fluorescent-lighted Veterinary Entomology Lab on campus.

Black flies were routinely taken off their sugar source at least 24

hours before planned feeding trials and maintained only on the water

provided by the moist cotton. Black flies were aspirated from the car-

tons or chilled in a refrigerator and removed with forceps and placed,

usually ane per vial, into seven-dram, clear plastic, cylindrical vials

covered with green, fine mesh (30/6.45 sq cm) netting at both ends and

held against the bare skin of the imrmobilized turkey. Room temperature,

relative humidity and feeding times were recorded.

Uninfected turkeys bitten by possible vectors were held in a fly-

proof room and the appearance of gametocytes in the peripheral blood

was monitored by blood smears obtained by wing vein punctures every 3

to 4 days. Blood smears were processed by air drying, fixing in

absolute methanol and staining in a 1:50 dilution of Giemsa solution

and distilled water. Flies were dissected in .9% physiological saline

and midguts and salivary glands were observed for the presence of

oocysts and sporozoites of L. smith. A few midguts were fixed in 10%

formalin, stained in Haematoxylin and mounted in balsam, with the

necessary intermediate steps for dehydration and clearing, in attempts





-53-



to more readily view the oocysts. Most midguts were air dried, fixed

in methanol and stained with Giemsa, as were the salivary glands. The

preparations were covered with permount and a cover slip for microscopic

examination. Other fly structures were placed in 10% Na0H (except the

wings) for 48 hours, rinsed in water and acid alcohol, placed in 100%

cellosolve for 15 to 30 minutes and mounted in balsam on a microscope

slide.














CHAPTER IV
RESULTS AND DISCUSSION



Simuliidae


General Comments


The primary efforts of this survey and ecological investigation

of the black flies of Florida were concentrated on the immature stages.

Over 700 adults were reared and pinned or mounted in balsam on slides

and a number of adults have been captured in traps or on host animals

(Tables 4, 6, 7 and 8) however the vast majority of specimens are

alcohol -preserved larvae and pupae. Over 1,100 four-dram vials con-

taining about 50,000 specimens from 1,080 individual, positive collections

which have been examined and identified are on hand. In addition,

records were obtained for Florida black flies from institution and

individual collections and are included under the appropriate species.

Table 1 is a list of the Simuliidae collected during this research

in Florida. Eighteen species representing two genera and six subgenera

are recorded. Figure 7 shows the 192 locations where I personally col-

lected specimens or obtained records from the collections of other indi-

viduals. One star normally designates a single collection site; in a

few cases a single star is used to indicate two collection sites adjacent

to each other such as a main stream and a side drainage flow to that

main stream. Each collection location is listed by county and site

number in the Appendix and the name and location of each stream is given.

The 50 counties from which black flies are recorded are listed in Table 2


-54-








Table 1. Florida black fly species.


Onephia (Chephia) ornithophilia Davies, Peterson, and Wood*

SimuZium (Byssodon) meridionate Riley

SimuZium (Byssodon) slossonae Dyar and Shannon

SimuZium (Eusimuiwnw congareenamrw(Dyar and Shannon)

SimuZium (Phosterodoros) diziense Stone and Snoddy*

SimuZium (Phosterodor~os) haysi Stone and Snoddy*

Sinulium (Ph~osterodoros) jenningsi Malloch

Simulium (Phosterodoros) jonesi Stone and Snoddy

Simutiurm (Phosterodoros) Zakei Snoddy*

Simulium (Phosterodoros) notiate Stone and Snoddy*

S~imultiwn (Phosterodoros) nyssa Stone and Snoddy

SiimuZium (Phosterodoros) tacodium Snoddy and Beshear*

SimuZiwn (Psitozia) vittatum Zetterstedt*

Simutium (Simuliwn) decorum Walker

SimuZiwon (Simutium) tuberosum (Lundstriim)

SimuZiwn (Simulium) verecundum Stone and Jamnback*

Onephia species undetermined No. l*

Simutium species undetermined No. 1*


New collection record for Florida.


































































Figure 7. Locations in Florida where black flies have been collected.










Table 2. Florida black fly distribution records by county.






Species



O O







County~~~~~~~ Sie

Alacua 3 x xx x x xx x x
Baker~Q 1 xx





Dade 1 x


Desoto
Dixie
Duval
Escambia
Flagler
Franklin
Gadsden
Glades
Gulf
Hamilton
Hardee
Hendry
Highlands
Hillsborough
Holmes
Jackson
Jefferson
Lafayette
Lake
Leon
Levy
Liberty
Madison
Manatee
Marion


x x
x x
x


x
x x
x
x
x x
x
x
X
x



x
x
x
x x
X
x
x
x
x
x x
x
x


X x
x xx
x x


x
x X


xX xx


x x x xx x
xX xx


x x x
x
x x


xX xx
xX xx


x
x xx


x xx
x x


x xx
x x x


x x
x
x


x x
X


x x xx
x x xX xx
x x X
x x xX x xx
xX x xx
x













Species


o 0








Osceola 1


Pinlla 1\ x



Sute 1 x x


Waul 1 xO~rO 3 ~


Wralng 4 x x xx


-58-


Table 2. (Continued)





























































By reading across or down from a selected species the number of times
the immatures of that species were collected together with the immatures
of another species is indicated by the figure at the intersection of the
two columns.


-59-


Table 3. Black fly associations based on collections of immature stages.*


26 1 4 4


84 3 1 38107 73
2 19 7

3 24
3 5
2 19 92
19 24 5 19 36


C. ornishophilia
S. meridionate
S. s~ossonae

S. congareenatum
S. diziense

S. haysi

S. jenningsi
S. jonesi
S. lakei
S. notiate

S. nyssa
S. taxodian
S. vittatum
S. decorum
S. tuberosum
S. verecundum

S. slpp. undetermined


2 2 32 10


52 15 186 55
6 2 49 23
21 4


73 4 21 8

2 1 40 11 3 253 44
150 15 54 19
23 1


7


38
1 107
73


92 36


8 44 10
8 9 50 33
9 67
4 50 6 78


2
2


32
10
No. 1


52 6 73 40 150
15 2 4 11 15


186 49 21 5 21 253 54
55 23 4 8 44 19


10 33


1 4


7 78


ar
s N


s s ~o
E~s
oEm


32





-60-


Table 4. Black flies captured in Manitoba traps.


Collection
times


1730-2030


1130-1930


1800-2000


1710-2020


1500-1745


0630-0930;
1730-2045

1630-1845


1600-1800


1600-1730


1500-1830


1530-1815


0700-1010;
1530-1800

1730-1845


0830-1430


1630-1805


1445-1710


Species
(all females)


vittartum


slossonae,
(Phosterodoros) spp.

s lossonae


slossonae


s lossonace


alossonae,
(Phosterodioros) spp.

slossnnae,
(Phosterodoros) spp.

s lossonae,
(Phosterodoros) spp.

s Zossonae


sZoss~nate


slossonae


stossonae,
(Phosterodoros) spp.

slossonae,
(Phosterodoros) spp.

slossonae


s lossonae,
(Phosterodoros)6 spp.

congrelcrenrum,
slossonae,
(Phosterodoros) CFpp


Date


1974: 3 May


30 May


28 June


28 June


6 July


11-12 July


16 July


16 July


19 July


19 July


24 July


26-27 July


15 Aug


17 Aug


15 Sep


1975: 24 Jan


Location


Univ. of Florida,
Site 34

Hatchet Creek
Preserve, Site 24

Lochloosa Creek,
Site 22

Hatchet Creek
Preserve, Site 24

Junction SR 225/
340, Site 8

Fisheating Creek,
Site 95

Lochloosa Creek,
Site 21

Hatchet Creek
Preserve, Site 24

Sandy Hatchet
Creek, Site 1

Univ. of Florida,
Site 34

Hatchet Creek
Preserve, Site 24

Fisheating Creek,
Site 95

Lochloosa Creek,
Site 22

Univ. of Florida,
Site 34

Lochloosa Creek,
Site ?22

Lochloosa Creek,
Site 22










Table 4. (Continued)


-61-


Collection
times


1000-1330


1100-1330


1000-1400



0930-1330


1030-1300


0930-1200


1400-1930


0730-1145;
1600-1910

1730-2000


0730-1200


0700-0830;
1500-1900

0830-1130;
1515-1715

0815-1315;
1620-2000

1625-1800


1400-1530


Species
(all females)


congareenarum,
s lossonae

congareenerumn,
s lossonae

s lossonae



slossonae


s lossonae,
(Phosterodoros) spp.

slossonae


s lossonae,
(Phosterodoros) spp.

sZossonae,
(Phosterodoros) spp.

stossonale


slossonae,
(Phosterodoros) spp.

slossonae,
(Phosterodoros) spp.

slossonae,
(Phosterodoros) spp.

slossonae


congareenarum,
slossonae

congareenrumro,
slossonae


Date


1975: 26 Jan


12 Mar


12 Mar


Location


Double Run Creek,
Site 43

Thomas Creek,
Site 159

8 km west of
Highway 115C on
Rt 90, Site 72

Double Run Creek,
Site 43

Turkey Creek,
Site 216

Junction SR 225/
340, Site 8

Hatchet Creek
Preserve, Site 24

Fisheating Creek,
Site 95

Lochloosa Creek,
Site 22

Fisheating Creek,
Site 95

Fisheating Creek,
Site 95

Fisheating Creek,
Site 95

Fisheating Creek,
Site 95

Turkey Creek,
Site 216

Double Run Creek,
Site 43


6


6


1


18


27-29


7


29-30


9-10


17-18


15-16


1976: 17


24













Collection Species
Date Location times (all females)


1976: 24 Mar Turkey Creek, 1640-1830 S. congareenzarum,
Site 216 S. slossonae

26 Mar Sante Fe College, 1030-1800 S. tuberosum,
Site 28 S. slossonae


-62-


Table 4. (Continued)












C. (C.)I ornithophilia

S. (B. ) meridionate

S. (B.)I slossonae

S. (E.)i congareenarum

S. (P.)I didiense

S. (P.)I haysi
------~I
S. (P.) jenningsi

S. (P.) jonesi
1 I
S. (P.) Lakei
I
S. (P.) notiate
I I

S. (P.) nssa

S. (P. ) taxodium

S. (P.) vittatum

S. (.S.)I decorum

S. (S.) tuberocum1

S. (S.)I verecundum
t
C. spp. No. 1

S. spp. No. 1


A S' O N D


J F M A M J J

Months


Figure 8. Seasonal occurrence of black flies in Florida.








with an indication of the species found in each county. Table 3 pre-

sents information on the frequency of association of black flies in

Florida's streams and rivers. Figure 8 shows the seasonal occurrence

patterns of the collected Simuliidae. Table 4 presents information on

species captured in Manitoba traps. Further reference to these figures

and tables will be made in discussing the individual species below.

Keys to the black flies of Florida, in all stages, are included.



Introduction to the Black Fly Keys


Characters which are important taxonomically on black fly larvae

and are noted on Figures 9, 10, and 11 include: the anterior, multi-

ple-rayed cephalic fans and their stalks (CF,CFS); the antennae (A); the

central dorsal section of the larval head capsule referred to as the

fronroclypeus or the cephalic apotome (CA) which bears usually dark

pigmented areas called head spots (HS); the ventral, setae bearing and

anteriorly toothed hypostomium or submentum (S); posterior to the sub-

mentum is the throat (or postgenal) cleft (TC) or gular notch which

differs in size and shape in the different species; laterally on the

thoracic region are a pair of organs called the respiratory histablasts (RH)

consisting of a number of coiled filaments whose number and branching

pattern are often diagnostic; the ventral proleg (VP); dorsally at the

posterior end the anal gills (AG), asmoregulatory organs, protrude and

may be simple and branlchless stalks or may have many branches and be

arborescent; behind the anal gills is located the anal sclerite (AS) or

X-piece which assists the larva in releasing its posterior from the silk

attachment patches; th7e cir-lfe or many rows of tiny anal hooks (AH) at




-65-


CF r A
CFS CA















Figure 9. Dorsum of the head capsule of a black fly larva (S. slossonae).















TC


Figure 10. Venter of the head capsule of a black fly larva
(C. ornithophi. iia) .





-66-


RO \


.*


Figure 12. Black fly pupa and cocoon (S. dixiense).


AS

Figure 11. Lateral view of two black fly larvae (S. dixiense).









the very end of the larva; and the ventral protrusions which occur on

some larvae and are called ventral or anal tubercles (VT).

Characters important on the pupa include: the filamented repira-

tory organs (RO); tiny setae-like structures on the thorax called

trichomes and posterior dorsal tail hooks (Fig. 12). On the

cocoon lateral apertures (LA) or windows occur anteriorly in the

Phosterodcros species and the texture and general regularity of the

cocoon is sometimes useful in separating species.

On the adult wing the presence or absence of hairs under the sub-

costal vein (SC); hairs on the dorsal, basal portion of the radius (R);

the presence or absence of a basal cell (BC); and color of hairs on the

stem vein (SV) are valuable characters (Fig. 13).

On the adult female head the shininess or pollinosity of the frons

(F) and its shape and that of the clypeus (C), the color of the antennal

segments (A), and the shape or size of the sensory vescicle of the

maxillary palps (SV) are useful taxonomically (Fig. 14).

Important characters of the male genitalia include: the appearance

of the ventral plate (VP) and the shape of its median portion; the shape

and relative lengths of the basimere (B) and distimere or clasper (D);

the presence or absence of a basal lobe on the distimere; and the number of

distal spines (DS) or teeth on the distimere (Fig. 15).

On the female hind leg the presence or absence of a dorsal groove

called the pedisulcus (P) on the second tarsal segment and the size of

the calcipala (CL), a flattened lobe on the inner side and at the apex

of the basitarsad segment, as well as presence or absence of a basal tooth

(BT) on the tarsal claws are important characters (Fig. 16j).

Structures useful for determinations in the region of the female





































Figure 13. A wing of the black fly Cnephia ornishophilia.


*


Figure 14. A frontal view of the head of a female black fly (S, notiaele.






























Figure 15. The male genitalia of a black fly, onephia omithophilia.







rC gBTI


Figure 16. The distal portion of the hind leg of a S. meridionatle
female.




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v g


Figure 17. The terminalia of a female S. meridionate.





-71-


genitalia (Fig. 17) are the size and shape of the genital. fork stem

(CF~S) an1d arms (CFA), the ovipositor lobes, each anal lobe (AZL) and

eachi cercus (CR).

A key to ther larvae of the blacki flies of: Florida.

(Photographiic illustrations of certain structures referred to in this

key and the keys which follow are included in the sections on the in-

dividual species. The larval key is primarily of value for later in-

stars. For further illustrations; refer to Stone and Jamnlhackk (1955),

Davies e~t al. (1962), Dood et al. (1963), StonE (1964), Stone and

Snoddy (1969~), Snoddy and Beshecar (1968) and Snotddy (11971, 1.976).

la. Hypostol:iuim convex, along anterior margin; head sp~ot pattern as in

Fig. 18. . .. ..... .. .. .. .. . C. ornrithop~hilia

16. Hypostomiumum cone~a\ or leve~l alo~ng tol anterio~r maergin~; the hcad

spot pattern not as in Fig. 18 ... .. .. ... . ... 2


2a. (Ib) Hiead spots light (white) .. .. .. .. . ... . 3

2b. Nlead spots dark or indistinct in a fulv~ous pattern . ... .. 5


3a. (2a) He~ad s5pot:, conpsristing of a cenitral.1 poste-io~r w~hte spot1 with

dark rays projecting and dive~rginge anterio~rly (Fig. 247, Stone and

Snoddy, 1.969; nol mature larva!e coll~ected~). . ... .S. mier~c~'idone

3b. Hea:d spot! s with! 3nterjior anld po~ster~ior- medial anld lalteral spots all

whiLe, spots vi-ib~le in thie usual pofitjions . .. .. .. .. 4


4a. (3b,) Meiccll an~terior and postecrior wh~ite hanld spots bor-dered by a

darki fulvous area on each sidle (F'ig. 99); Iespiratory his8tchlost

wilhl 8 fijlownts~n .. . ,. .. .. . . . ,'. decoruim

Ab. Nol dist inc. dairki fulvous border b~y rcontra? l he spots; histoblnst

wJith 6 filamints; .... . .. . . . . S. :'''^wrecundu









5a. (2b) Gular notch in the-form of a shallow inverted -v; headlspots

as in F~ig. 121 ... .. .. .. ... . .. C. species No. I

5b. Gular notch rectanngular, sub-rcctangular or sagiittiform. . .. 6


6a. (Sb) Cul~ar nlotch broadly sagittiform, ext~eundin at least across 8

of the venter of the head capsule, as widle as long .. .. .. 7

6b. Cular notch not Ragittiformi or else longer than wide .. .. .13


7a. (6a) R~espiracory histabla~sts consisting of 10 filomntins 9 of which

arise fr~om a thick basal 10th (Fig. 60); aaterior medial head spots

often weak . .. ... .. .. ... .. .. S. Jonesi

7b. Respiratory histablast not as above; a.ll head spots usually dark,

distinct .. .. .. ... .. .. .. .. .. .. ... 8



8a. (7b) Respiratory histroblast with 6 filaments; anal tubercles not

prominent. .. .. .. .. .. .. ... .. . .. S. notiate

8b. Respiratory histoblast within more than 6 filarmen~ts; anal tubercles

promninenlt........ ... ... .... ........9


9a. (8b) Respiratory histoblast wait-h 7 filanones. . . .. S. haysi

9b. Respiratory histablast with more than 7 filame-ncs. .. .. .10


10a. (9b) Respiratory hristoblast with, 8 filaments. . ... S. tiaOicodiim

10b. Respiratory histohlast with more than 8 filamnents. . ... .11


11a. (10b) Rlespiratory histablast ulith 9 ;ilanments .. .. .. ,S. Zakei

11~b. Respiratorq histohlzst wJith 10 finments . .. .. .. .12


1.2a. (1lb) 10 recspira::ory h-iotablast filamennts w~ith a pattern as in

Fig. 81.; a1nal tuberele~s piromuinenl 91nd CocaiI. .. . S. niYouC

1.2b. 10 respiratory hiistchlast filametnts w~ith a palttorn as in Fic. 53;

anal tuibercler sma1 :l and ronded lc.. . .. . .. jennringsjri










13a. (6b) Gular notch long extending b the distance or more to thle teeth

of the submentum, sagittiform or subrectangular, pointed or rounded

anteriorly. .. ... .. ... .. ... . . . 14

13b. Gular notch extending less than k the distance to the teeth of sub-

mentum, subrectangular. ....... ...... .. 16


14a. (13a) H-ead spots indistinct in fulvous area; gular notch usually

with parallel sides; anal tubercles inconspicuous . S. tuberosure

14b. Head spots distinct; gular notch with curved margins, elongate

sagittiform; anal tubercles prominent . . . . . . 15


15a. (14b) Re~spiratory histablast with 6 filaments; larvae reddish.

. . . . . * S. slossonae

15b. Respiratory histoblast with 10 filamren~ts; larvae ye~llow.

. . . . . . . . . . - S, clixience



16a. (13b) Respiratory histoblast filaments 4 in number; gular notch

longer than wide. . .. ... . .. . . S. species No. 1

16b. Respiratory histoblast. filaments numbeLr more than 4; the gular

notch not longer than wide. .. .. .. .. ... . .. .. 17


17a. (16b) Respiratory histablast with 12 filaments; the anal tubercles

are large, prominent; a medium~-sized larva, 6 mm~ long.

. . . S. conga~ailreeam

17b. Respiratory histoblast withi 16 filaments; the anal tubercles are

absent or inconspicuous; a large larva, 8-9 mml long . S. viCttatumi






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A key to the pulPae of the black flies of Florida.

(No pupae of Cnephiia species No. 1 have been observed.)

la. Cocoon a loose mass of silk, indistinct shape; strong dorsal books

at the posterior end of the pupa. . . .. .. C. ornithophilia

lb. Cocoon a distinct slipper or pocket shape, strong dorsal hooks

absent. .. .. .. .. .. .. .. .. .; . . .. . 2


2a. (lb) Cocoon with large anterior, lateral apertures .

2b. Cocoon uniform without large lateral apertures. ...



3a. (2a) Pup~a wath 6 respiratory filaments on each side;

antero-ventrally comlpletely joined (FiG. 75). ....

3b. Pupa with miore than 6 filaments ...........



4a. (3b) Pupa with 6 filaments rising off a strong basal

(Fig. 48) . . . . . . . . . . .

Ab. Pupa with more tha7n 7 fil.aments ...........



Sa. (4b) Pupa with 8 filamecnts with four pairs of 2. ..

5b. Pupa wit-h more than 8 fi'l~anclts ..........


. . . 3

. . .10


cocoonI

..S. notiate





7h one

...S. haysi





.S. Zaxodium

. . .6


6a. (5b) Pupa with 9 filaments in the pattern 2, 2, 2 and 3 from the

dorsal. .. .. ... .. .. .. .. .. .. .. .. S. lakei

6b. Pup3 with 10 filaments. ... .. .. ... .. .. .. 7



7;1. (6b) Pupa wJithl 9 filaments rising from a thick basal 10h one

(Fig. 60) ... ... . .. ... . ... .. ionesi

7b. Pupal respiratory organ lacking a str-ong basal filnment . .. 8



88. (7b) Pupa w~itn 10 respiraltoryy filamecnts whichh rise as 5 pairs, the

petiole for filaments 7 nnd 8 (from; thIe dorcal) noticiably longer










and stouter than the other petioles (Fig. 12) .. .. S. diziense

8b. Pupa with 10 filaments, some of which~ rise in pairs others in

triplets. .. .. .. .. . .. . .. . .. 9



9a. (8b) The lower more ventral respiratory filaments on long petioles,

filaments long. .. ... ... .. .. .. .. .. S. nyssa

9b. Lower filaments rise close to the base, petioles short, filaments

short .. .. .. ... ... .. .. . S. jcinnngsi



10a. (2b) Pupa with 4 respiratory filaments. .. .. S. species No. 1

10b. Pupa with more than 4 filaments .. .. ... .. .. 11



11a. (10b) Pupae with 6 filaments in the respiratory organ1. .. 12

11b. Pupae with more than 6 filaments. . . ... . 14



12a. (11a) Pupal cocoon with concave anterior edge (in lateral view);

respiratory filaments rising from long petioles (Fig. 28)S. slossonae

12b. Pupal cocoon with convex or straight anterior edges; petioles

short ... .. .. .. ... . .. .. . ... .. .3



13a. (12b) Respicatory filaments widespread, base of filamlents 5 and

6 sharply separated from banse of 3 and 4,filaments long (Fig.115).

. . . . . . * * S. vercun dw~n

13b. Respiratory filaments not: widespread, bases about equidistant,

filaments short (Fig. 108). ... .. .. ... .. S. :ubcr'oswni



14a. (11h) Pupa with 8 respiratory f~laments; cocoon with rouGh text~ure.

. . . . . . . . . . S. accr:C r nl

14b. More than 8 filaments . .. .. . .. .. .. . 15



.15a. (141,) Pupa with 12 respiratory filaments; cocoon with long anterior-





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dorsal projection (Fig. 35).. . .. ... .. .. cong'areenarurr

15b. Pupa with more than 12 filaments; cocoon lacking dorsal projec-

tion .. .. .. .. .. .. .. .. . .. . .. .. 6



16a. (15b) Pupa with 16 filaments in 8 pairs . .. .. .. S. vittatus

16b. Pup3 withr more than 20 filaments in pairs and in 3's .S. meridionale




A key to the adult male black flies of Florida.

(Adapted and modified from Stone and Enoddy, 1969)

la. Second tarsal. segment of the hind leg without a pedi~sllcus but a

shallow~ depression may be present; radius vein with hair~ dorsally

on the basal segment; large basal cell present . .C. ornithophilia

lb. Second tarsal sfement of the~ hind leg with a deep pedisulous; base

of radius withi or without hair dorsally; large basal cell absent.





2a, (Ib) Basal portion of radius with hair dorsally S. congareeniar~um

2b. Basal. portion of radius without hair dorsnlly. .. .. .. .. 3


3a. (2b) D~istimere~ short, stout, with; 3 or mo~re tceeth .. S. visttumw

3b. Distimore long, with 1 tooth or none .. .. ... .. .. 4


4a. (3b) Distimere with a rounded 10be on the inner margin near the

base .. . . .. .... .. ... .. ... .. 5

4h, Distimefre without a lobe or basal projection .. .. .. .. 6


Sa. (42) Distimuere with ba~sal lobe bearing a number of stout, spine-

Itke scenec . .. .... .. .. .. ... .. S. tuber'osur

5b. Distime~re with basal lobe bearing finec hairs only. . S. stossonae










6a. (4b) Ventral plate In ventral view broadly rounded. S. meridionale

6b. Ventral plate in ventral view more narrow, compressed from th~e

sides. . .. . ... . .. .. .. . .. .. 7



7a. (6b) Ventral plate with basal arms hearing distinct lateral pro-

jactions; posterior third of sc~utum shiny with indistinct hairs. 8

7b. Ventral plate with basal arms without distinct lateral projections;

posterior quarter or less of scutum shiny with some strong erect

hairs. ... .. .. ... . .. ... .. .. . .. 11



8a. (7n) Ventral plate in ventral view with median portion longer than

wide and parallel-sided or nearly so; scutumn with silver spots

narrow, oblique; thle dark area be~tween the spots broadens anteriorly.

. . . . . . . . . . . . . . S. dizience

S. jenningjsi

S. notiate


8b. Ventral plate in ventral view with median portion not longer than

wide and' widened toward the end; anterior silver spots ;nd inter-

vening dark area variabLe. .. .. ... .. .. ... .. 9



9a. (8b) Scutuml with large anterior silvery spotsa each extendingi about

one-third the distance across the front of thle scutum; thle dark area

between the spots na1rrows strongly to thle anterior margin. .S. Jone~d

S. txodiuim


9b. Scutumi with smanller silvery spots; ilhe dark area betweenr th:e spots

is broad convergies little, and is narrow~est a-bout mindway along

the l~ength of the spot; .. .. .. .. ... . S. hiaUGL

S. lakei.

S. nuiscal









10a. (7b) Ventral plate in ventral view narrow, V-shaped, middle

region tapers almost to a1 point; with a1 ventral keel. S. decorumr

10b. Ventral plate broader, not as strongly compressed in the middle

section; ventral keel absent. .. .. .. .. .. S. vereoundlun




A key to the adult female black flies of Florida.

(Adapted and modified from Stone and Snoddy, 1969)

la. The second hind tarsal segment lacks a pedisulcus, although there

may be a slight depression; the basal cell is present and the basal

portion of the radius bears setae dorsally . .. C. ornlithophiliai

lb. The second hinid tarsal segment with a deep pedisuicuis; large basal

cell absent and the basal portion of the radius bears or lacks setae

dorsally (Simu~7~ium.. ... ... .... . . . . ..


2a. (lb) Radius vein with hair dorsal~ly on the basal section.

. . . .. . . . .. S congareelnarumn

2b. Radius without hair dorsally on the babal section. .. .. .. 3


3a. (2b) Tcrsal claw with a prominent basal. tGOoc .. .. .. .. 4

3b. Tarsal claw simple, without a prominent basal tooth. .. .. 5


4a. (3a) Fr-ons gray pollinose .. ... .. .. .. S. mieridionale

Ab. Frons shiny black. .. .. ... .. .. ... .S. slossonna


Sa. (3b) Frons and terminal abdominal targites shiiny black or dark

brown. ... . .. .. . . .. ... . .. . .. 6

5b. Fron~s and tecrminal abdominal tergites at least lightly pollinose.10


6a. (Sa) Sub~cosr-al vein with a row of hai~rs ventral3y; scutumn

subshiny .. .. .. .. ... . . .. . .. .7










6b. Subcostal vein without hairs ventrally; scutumi shiny .. .. 8


7a. (6a) Fore tibia with a narrow gray-white patch not more than one

third the width of the tibia; inner margins of ovipositor lobes

fairly st-raigiht. .. .. .. .. .. .. .. ... S. tubhero~swn

7b. Fore tibia with a brilliant white patch which covers one half or

more of the tibia; inner margins of the ovipositor lobes concave

enclosing anr oval area .. .. ... ... .. S. ver~ecundum


8a. (6b) In anterior view the scutum displays a pair of fairly distinct

rounded pollinose spots with a darker area of the scutum in between;

hairs on the stem vein are dark brown to black . .. S. jenningsi

S. notiate

S. nyssa

S. taxodium

8b. In anterior view the scutum is not pollinose or the pollinosity

occurs as a diffuse area along the front and sides .. .. .. 9


9a. (8b) Clypeus about as wide as long. . .. .. S. jonesi

S. Zakei

9b. Clypeus longer than wide . .. . .. .. .. S. dixiense

S. haysi


10a. (5b) Scutum silvery gray with dark brown markings; abdomen with a

black and light gray pattern .. .. .. .. .. S. v~ittatusm

10b. Scutum uniform brownish gray without contrasting dark brown manrkings;

abdomen black with thin g~ray pollinosity without a pattern.

. . . . . . . . . . S. decolmm





-80-


Introductionto th Individual Species Sections


The followingf sections deal specifically with the black fly species

found in Florida. References are listed below each species name which

provide synonyms for the currently accepted name and additional sources

of descriptions andi figures. The descriptions which follow the brief

taxonomiy portions are intended to point out outstanding or diagnostic

characters and are accomlpanied with photographic illustrations. Under

"Florida Observations" below the heading "Stream Parameters" A summary

is given of the dimension, pH, temperature, and velr.city figures for

streams which contained each par-ticular species. i~n the sectionn on

Florida collection records the numbers i:amediatel~y inl!.owing the county

names refer to individual collection siteS identified inl the A~ppendix.

The code for thle collection records is as Follow~s: S refers to small,

very young larvae with no or weakly developed respiratory histoblasts;

M refers to medium-aged larvae with distjinct but white histablasts;

Refers to large, mature larvae withi dar:k histobl~asts; P refers to

full pupae or pupal exuviae; C refers to ucocons; anld A1 refer~s to male

or female adults. Tlh- majority of records refer to specimens the

authonr personally collected; in those instances where specimens were

gathecred by other individuals, the collector is noted. On the indi-

vidual species ma~ps wJhen a record is included with the only locality

information beingi the county the mark for that record on the ma~p is

placed in thle center of the :ounty. Immat~nure, very young larvae

lacking well-develouped respirnatry h~istobtasts in the subgenus

Phol~sterodoros~? are difficult if not impossible to differentiate as: are

the adlul~t fema;les. Records for t~he P~hostr:: odo:. l~.i ronpeces are based~

prima~rly oin mature larvae and p~uPre. Only at locntionss whero









essentially a single Phosterodoros species was found to exist are

records for earlier stages included. Records for undetermined species

are listed as S. (Phosterodoros) spp. in the Manitoba trap results

(Table 4). Dr. E.L. Snoddy kindly examined immature and adult specimens

of S. jenningsi, S. lakei and S. tarodium~ from Florida and confirmed my

identifications.



Onephia (Cnephia) or-nithophilia Davies, Peterson, and Wood


onephia ornitshophiziai Davies, Peterson and Wood, 1962, Proc. Entomol.

Soc. Ontario 92: 102 (female).

Onephzia ornithoph2iia Stone and Snoddy, 1969, Auburn Univ. Agr. Exp.

Sta. Bull. 390: 25 (female, larva).


Taxonomy. Davies et al. (1962) first described a female of this

species. The holotype was collected from a blue jay at Algonquin Park,

Ontario and was deposited in the Canadian National Museum. Paratypes

are located in the U.S. National Museum. Stone and Snoddy (1969) in-

dicated the male of C. orn)ithophlZ~ia was not known and that the larva

and pupa were apparently indistinguishable from those of C. pecuaruo.

Description. The larva is large, about 8 mm long with a grayish

brown abdomen and a light yellow brown head capsule. The cephalic

spots are dark with the median groups of about 20 forming one long

continuous row (Fig. 18). The submentum is convex apically and bears

small teeth. The gular notch has fairly parallel sides, is broadly

rounded anteriorly and extends less than 1/3 the distance to the sub-

mental teeth (Fig. 10). The cephalic fans each contain about 60 rays

with long hair-like spines. The anal tubercles are absent.





-82-


Figure 18. The head spots of a larva of C. ornithophilia.


Figure 19. The pupal exuvium and cocoon of C. ornithop~hilia.





-83-


The pupa bears strong dorsal posterior hooks and is located in an

irregular, loosely woven cocoon. The respiratory organs each contain

about 30 filaments (Fig. 19).

This is believed to be the first description of the male. The

wings are 3.75-4 mm long. There are hairs dorsally on the base of the

radius. The first segment of the flagellum is the longest, about twice

the length of the other segments. The scutum is dull brownish black with

numerous short golden hairs. The scutellum is reddish brown and bears

long dark hairs. The legs are almost uniform yellow brown. The second

hind tarsal segment lacks a pedisulcus. The abdomen is dull brownish

black dorsally and lighter gray or light brown ventrally. The ventral

plate in ventral view is broad and the tapering distimere which is

almost equal in length to the basimere has a single tooth at its apex

as in Fig. 15.

Plesiotype: Male, with associated pupal exuvium and cocoon,

NW 23rd Ave. and NW 83rd St., Gainesville, Alachua Co., Florida, 2

February 1975 (Pinkcovsky), to be deposited in the U.S. National Museum.

The wings of the female are about 4.5 mm long. The base of the

radius is covered with setae and a basal cell is present on the wing.

The frons bears short yellow hair. The pedisulcus is essentially absent

and the prominent tooth on the basal claw has convex margins (Fig. 20).

The apex of the abdomen is shiny. The genital fork has widespread

narrow arms as in Fig. 21.

Distribution. Stone and Snoddy (1969) indicate C. orni-thophiia

occurs in Louisiana, Mississippi, South Carolina, Virginia, and Ontario.

Tarshis and Stuht (1970) report this species from Mlaryland.

Life History. Stone and Snoddy (1969) mention that C'. ornith~ophilla







































Figure 20. Tarsal claw of a female of C. ornithophilia.


Figure 21. Genital fork and terminalia of a
female.


C. ornithophilia









is probably univoltine. Eggs collected in stream bottom samples and

placed in laboratory rearing tanks yielded first instar larvae three

days later (Tarshis, 1973). Larvae have been collected between 11

January and 18 March in South Carolina and adult females were reared on

6 February from pupae; females have been collected from 6 February to

1 May in Louisiana and Mississippi (Stone and Snoddy, 1969). Tarshis

(1973) working in Maryland found larvae from 14 November to 29 April,

pupae from 11 January to 22 April and adults from 2 to 17 March. Tarshis

and Stuht (1970) captured adult C. ornithophitia between 28 February

and 1 April in emergence cages over a stream in Maryland. When first

to third instar larvae were collected and reared in the laboratory in

aerated water at 15-220C pupae appeared after 2 to 21 days and adults

emerged 4 to 23 days after introduction of the larvae (Tarshis, 1973).

Tarshis (1973) found adults emerged as soon as 2.5 hours after pupation

[very difficult to believe] and that the number of males emerging ex-

ceeded the number of females emerging only on the first day.

Ecology. Tarshis and Stuht (1970) found massive numbers of larvae

on leaves, rocks, and twigs in a stream that flowed .153 to 1.0 m/sec

(.49-3.3 ft/sec) from a pond through an oak and willow woods. TIhe

stream was .92-1.53 m (3-5 ft) wide, 2.5-20 cm (1-8 in) deep and had a

pH reaction between 6.9 and 7.4. In a similar stream that was slightly

deeper and 1.22-3.66 m (4-12 ft) wide, larvae were collected at water

temperatures of .5-15oC (Tarshis, 1973). Tarshis (1973) mentioned that

pupae easily suffered injury and that development to adults did not

occur when pupae were removed from their substrate. Larvae of C. muitaba,

P. gibsoni, S. decorum, S. tuberosumn, S. venustum, and S. vitt~atum were

collected with C. ornithophilia in a pond runoff stream where









C. ornithophilia predominated (Talrshis antd Stuh~t, 1970).

Habits. Tarshis and Stubt (1970) suggest that C. ornithophilia

ov-iposits on the stream surface and the eggs sink to the stream bed.

Bennett (1960) in Ontario recovered C. ornithophilia (as CnephiJa "U")

fromt jays, hawks, robins, sparrows and other woodland birds. Tarshis

(1972) fed C. onnithophiia on geese and ducklings in the laboratory

and transmitted L;. simondi to ducklings by the bite of the fly. Tarshis

(1973) did not collect any C. ornithophi~lia from ducklings exposed on

the banks of a stream containing larvae and pupae of the black fly and

suggests that thle target hosts were inappropriate or perhaps that the

flies were absent at ground level, and concentrated in the canopy.

Florida observations.

Stream Parametrers

Width Depth pH Temperature Velocity

Mleanl: 2.57m 23.64 cm 4.7 14.70C (58.60F) .45 m/sec (1.47 ft/sec)

Mlin: .3 1.3 3.6 8.3 (47) .23 (.75)

Max: 11 90 6.6 24.4 (76) 1 (3)


C'nephia ornithophilia-lc is reported here for the first timne from

Florida. Both Stone (19)65) and Stone end Snoddy (1969) mention that

C. pecuarum occurs in Florida. Key chiaracters, such ats the shape of

the tooth on the tarsal claw of the female, on specinens I obtained in

Florida differed from those of C. pccuarwn.m In the U.S. National

Museum I located one adult On~ephia specimen from Florida, listed for

Site 204 below,, which was a male that had been labeled as C. pecuarum

but C. ornithophiliadn was pe~nciled inl underneath.. Adult and immature

specimens whlichl I submitted fro~m five collections representingli two welst

Florida (Hol~mes and Liberty) and one east F~lorida (Alachua) counties werre








identified by G.E. Shewell of the Canadian Biosystematics Research

Institute as C. omnithophilia.

Onesphia ornithophilia was collected from 31 sites in 14 counties

in Florida (Fig. 22). Most of the records represent collections of only

a few, usually young larvae with small, pale histoblasts, but the larger

overall body size compared to other species and distinctive head spots

and gular notch are discernible even in young larvae. Larvae, pupae

and associated adults were obtained at three sites 28, 119 (Fig. 23),

and 147). This species was first collected in the streams on 28 October

and last collected on 18 April. Most collections occurred and the

largest populations were encountered during December through March.

Collections of C". ornithophilia at a number of sites were made during

one winter month and the species was not found again until about the

same time the following year. It appears that C. ornithophilia is

univoltine in Florida. This species occurs in cool, fairly shallow

flows, usually at most a few meters wide, most frequently with a velocity

of .305-.61 m/sec (1-2 ft/sec) and a pH which normally is below 5. Im-

matures were also collected at Lebanon Station (Site 139) and Yellow

Water Creek (Site 70) where the pH approaches neutrality. At most sites

there was considerable vegetation especially trailing grass in the stream

to which the immatures were attached. At Blues Creek (Site 2) and Site

28 sparce vegetation was present. Site 28 originated in a small swamp

just upstream, was about .5 m wide, 10 cm or less deep and tumbled down

a root-filled and rocky bed through woods. In January a heavy popula-

tion of C. ornithophilia covered rocks, dead tree leaves, pine needles,

and a few blades of trailing grass. Masses of 40 or more larvae en-

circled twigs about .6 cm in diameter in the current. Most of the




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