Group Title: Identification, characterization, and soil mite vector relationships of Pseudomonas marginata (McCulloch) Stapp /
Title: Identification, characterization, and soil mite vector relationships of Pseudomonas marginata (McCulloch) Stapp
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Title: Identification, characterization, and soil mite vector relationships of Pseudomonas marginata (McCulloch) Stapp
Physical Description: xv, 146 leaves : ill. ; 28 cm.
Language: English
Creator: Noble, William Estep, 1940- ( Dissertant )
Stall, Robert E. ( Thesis advisor )
Engelhard, Arthur W. ( Reviewer )
Zettler, P. William ( Reviewer )
Poe, Sidney L. ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1974
Copyright Date: 1974
Subjects / Keywords: Pseudomonas   ( lcsh )
Plant Pathology thesis Ph. D
Gladiolus -- Diseases and pests   ( lcsh )
Dissertations, Academic -- Plant Pathology -- UF
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
Abstract: Florida isolates of Pseudomonas marginata (McCulloch) Stapp, the causal organism of bacterial neck.-rot and scab of gladiolus ( Gladiolus hortu lansus Baily) were collected and subjected to pathogenicity, physiological, nutritional, and bacteriophage-typing tests. Comparisons were made with authentic type culture isolates and data in existing literature to evaluate their usefulness of the tests for identification and characterization of naturally occurring pathogenic isolates of P. marginata . A method was developed by which large numbers of P. marginata isolates could be rapidly evaluated for pathogenicity. Environmental factors such as presence of free-water and high temperature were found necessary for significant disease development. Isolates of P. marginata were found to be positive for tobacco hypersensitivity when tested at high inoculum levels (10^ cells/ml) and at optimal bacterial growth temperatures of ca. 35 C -The nonfluorescent character of this "bacterium's pigmentation was confirmed. The majority of pathogenic isolates formed a white precipitate when cultured on nutrient agar. This characteristic proved useful as a marker in mite/bacteria retention studies. A bacteriophage was isolated from a neck-rot diseased gladiolus and found to be specific for isolates of P. marginata collected in this study. Bacteriophage typing proved usefal in screening for pathogenic isolates of P. marginata . The bacteriophage was observed by electron microscopy and partially characterized. Tnree species of soil-inhabiting, astigmatid mites common to diseased gladioli in Florida were studied as potential vectors of P. marginata. Anoetus feroniarum DuFour, Rhizoglyphus robini Claparede, and Tyrophagus putresentiae Schrank were observed under laboratory conditions for their feeding preference and population dynamics on selected fungal and bacterial substrates isolated from diseased gladioli. Anoetus feroniarum exhibited a preference for bacterial substrates. The hypopus stage was observed to be the most readily attracted stage of this mite's life cycle. Rhizoglyphus robini exhibited a primary affinity for forma specialis of Fusarium oxysporum , with a secondary affinity for Stroma - tinia gladioli. Rapid population increases were noted when A. feroniarum fed on P. marginalis and P. marginata , and when R. robini fed on Fusarium . Tyrophagus putresentiae was observed in low populations on both fungal and bacterial substrates confirming the polyphagous feeding habit of this mite in that it feeds on other things. Anoetus feroniarum and R- robini were investigated for their ability to acquire and disseminate P. marginata . The hypopus was considered a possible source of dispersion of P. marginata under natural conditions. Both species of mites were forcibly exposed to pure cultures of P. marginata and tested for passage of this bacterium internally and externally on their body. Neither species gave evidence of retaining viable cells longer than 5 days. The hypopus was eliminated as a source of F^ marginata inocula. Dissemination by A. feroniarum of P. marginata from diseased gladioli was negative. This evidence tends to eliminate A. feroniarum as an active vector of P. marginata. It is suggested that this mite species occupies an ecological niche in which it subsists on bacterial flors . Rhizoglyphus robini , while not attracted to, nor feeding on P. marginata , was positive for a low level dissemination of P. marginata from neck-rot diseased gladioli. Tnis species possesses mouthparts more adapted for possible infliction of wounds in soft tissues of young, newly developing cormels. Information gained in this study suggests further study of R. robini, as well as other prevalent soil-inhabiting insects and nematodes as possible short-term vectors of P. marginata in the rhizosphere of newly developing gladiolus cormels.
Thesis: Thesis (Ph. D.)--University of Florida, 1974.
Bibliography: Includes bibliographical references (leaves 136-141).
General Note: Typescript.
General Note: Vita.
Statement of Responsibility: by William E.Noble.
 Record Information
Bibliographic ID: UF00098176
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000425253
oclc - 37999885
notis - ACH3752


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