Title: 5 beta-cholestan-3 beta-ol an indicator of fecal pollution
Full Citation
Permanent Link: http://ufdc.ufl.edu/UF00097677/00001
 Material Information
Title: 5 beta-cholestan-3 beta-ol an indicator of fecal pollution
Physical Description: x, 106 leaves. illus. 28 cm.
Language: English
Creator: Kirchmer, Cliff Joseph, 1939- ( Dissertant )
Singley, Edward E. ( Thesis advisor )
Brezonik, Patrick L. ( Reviewer )
Fox, Jackson L. ( Reviewer )
Furman, Thomas de S. ( Reviewer )
Winefordner, James D. ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1971
Copyright Date: 1971
Subjects / Keywords: Environmental Engineering Sciences thesis Ph. D.
Dissertations, Academic -- UF -- Environmental Engineering Sciences
Water -- Analysis   ( lcsh )
Water -- Pollution   ( lcsh )
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
Abstract: Total coliform and fecal coliform organisms have been used in the past as indicators of fecal pollution. However, these indicators have shown many inadequacies, so that the National Technical Advisory Subcommittee on Public Water has stated that "It is essential that raw-water sources be judged as to suitability by other measures and criteria than coliform organism concentrations." The study reported in this dissertation was an investigation of a chemical indicator of fecal pollution, 56-cholestan-33-ol (coprostanol) . Experiments were performed to improve and shorten existing methods of analysis, as well as to determine the applicability of coprostanol as a water quality indicator. It was found that the free sterol could be chromatographed by using an acid-washed, silanized, diatomaceous earth as column support for SE-30. Improved cleanup of water samples was obtained by washing hexane extracts with acetonitrile saturated with hexane. Results indicated that coprostanol present in sewage is present primarily as the free sterol, with only approximately 10 percent present in the form of esters. Evidence was presented indicating that the removal of coprostanol by sewage treatment is primarily due to biodegradation by microorganisms. Determinations were made on the decay rate of coprost?.nol in laboratory samples of both chlorinated and unchlorinated sewage effluents. It was shown that samples must be preserved with sulfuric acid or by cooling to avoid biodegradation of coprostanol by microorganisms while in storage. Field surveys were made on the receiving streams and lakes of two sewage treatment plants in Gainesville, Florida. Free sterols were measured and found satisfactory as indicators. No correlation between coliform indicators and coprostanol was found in these surveys, primarily due to the effect of chlorination on coliform organisms. It was concluded that free coprostanol can be used as an indicator of fecal pollution in those cases where the fecal coliform tests are inadequate.
General Note: Manuscript copy.
General Note: Vita.
Thesis: Thesis - University of Florida.
Bibliography: Bibliography: leaves 98-105.
 Record Information
Bibliographic ID: UF00097677
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 001024914
oclc - 18000292
notis - AFA6829


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