Group Title: uptake of free fatty acids from sea water by a marine filter feeder, Crassostrea virginica /
Title: The Uptake of free fatty acids from sea water by a marine filter feeder, Crassostrea virginica
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Permanent Link: http://ufdc.ufl.edu/UF00097520/00001
 Material Information
Title: The Uptake of free fatty acids from sea water by a marine filter feeder, Crassostrea virginica
Physical Description: ix, 120 leaves : ill. ; 28cm.
Language: English
Creator: Bunde, Terry Allen, 1947- ( Dissertant )
Fried, Melvin ( Thesis advisor )
Allen, Charles M. ( Reviewer )
Gurin, Samuel ( Reviewer )
Carr, William ( Reviewer )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 1975
Copyright Date: 1975
 Subjects
Subjects / Keywords: Oysters   ( lcsh )
Fatty acids   ( lcsh )
Biochemistry and Molecular Biology thesis Ph. D   ( local )
Dissertations, Academic -- Biochemistry and Molecular Biology -- UF   ( local )
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
 Notes
Abstract: The ability of the American oyster, Crassostrea virginica to remove naturally occurring dissolved free fatty acids, inconcentrations approximating those found in sea water, was investigated using radioactive isotopes of palmitate, stearatc, and olcate. Petroleum ether (30 - 60°C) extracts of the sea water from a Florida Gulf Coast estuary contained up to 280 μg of total lipid material per liter including 77 yg of free fatty acid. Thefatty acids, separated by gas liquid chromatography, were predominatly saturated with even carbon numbers. The major fatty acid present was palmitate. The animals were shown to remove labeled palmitate from sea water by measuring the appearance of the radio-activity iu the chloroform extractable material. The uptake process was shown to be physiological and not chemical adsorption onto shells. This assimilation was Inhibited ,7±za 200 ml-I sodium cyanide. The temperatcre dependence of the uptake process vas investigated at 20, 25, 30, and 35^C. The rate of uptake of 50 pm celite particles carrying adsorbed radioactively labeled stearate and palmitate demonstrated that the process of filtration feeding was not responsible for the removal of freely dissolved fatty acid.. The rate of uptake of celite bound material was delayed by 3C minutes when compared to the uptake of an equal concentration of dissolved material. The kinetics of the uptake into chloroform extractable material was investigated for palnitate, stearate, and oleate. Both palpitate and stearate shoved saturable uptake systems as determined from reciprocal rate-concentration plots. The rate of uptake of both acids markedly increased when nicellar concentrations of the fatty acids were reached. The rate of uptake of oleate was much less than that for palmitate and stearate, and was not saturable at natural concentrations. The rate of uptake into isolatable lipid classes was investigated; the major species labeled were phosphatidyl choline, triglycerides, and cholesterol. The rates of incorporatron of palmitate into phosphatidyl choline and stearate into the total polar lipids were determined. Oleate was shown to effectively inhibit the uptake of stearate in competition experiments, but no effect was seen by oleate on the palmitate uptake. Increased oleate concentrations were shown to promote palmitate •uptake. Turnover rates for various lipid classes were determined by labeling with sodium. ^'acetate, removing the label, and following the decrease in specific activity of each lipid with time. The contribution of the uptake process to the total metabolic needs of the animal was estimated. The impact of such lipid uptake studies was discussed in light of municipal sewage and petrochemical pollution of natural oyster habitats as well as the selection of oysters as a possible aquaculture species.
Thesis: Thesis--University of Florida.
Bibliography: Bibliography: leaves 116-119.
Additional Physical Form: Also available on World Wide Web
General Note: Typescript.
General Note: Vita.
Statement of Responsibility: by Terry Allen Bunde.
 Record Information
Bibliographic ID: UF00097520
Volume ID: VID00001
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: alephbibnum - 000167707
oclc - 02864490
notis - AAT4098

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