Group Title: Progress report. Caribbean Coastal Pollution Project
Title: Progress report
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Permanent Link: http://ufdc.ufl.edu/UF00095631/00002
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Title: Progress report
Physical Description: Serial
Language: English
Creator: Caribbean Coastal Pollution Project
Publisher: UNU-INWEH
Place of Publication: Hamilton, Ontario, Canada
Publication Date: January-July 2009
Copyright Date: 2009
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Bibliographic ID: UF00095631
Volume ID: VID00002
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.

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Caribbean Coastal Pollution Project (CCPP)

Assessment, Monitoring and Management of Persistent Organic Pollutants (POPs) and
Persistent Toxic Substances (PTS) in the Coastal Ecosystems of the Wider Caribbean Region


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Caribbean Coastal Pollution Project


Second progress report on activities
Report 1.2


UNU-INWEH

(1 January 2009 31 July 2009)


Prepared by Hanneke Van Lavieren


UNU-INWEH


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Caribben Coastl PoluUon Projc


Status of Project Activities

This second progress report covers the period
January-August 2009. The first interim progress
report covered the period 1 November 2007 to 31
December 2008 and is available at:
http://www.inweh.unu.edu/Coastal/POPs/
ProgressReport2007-08.pdf.

Overall the project is moving forward quite well
despite some delays in the white grunt monitoring
activities, the analysis of samples and the equipment
upgrades of the laboratories.

We expect that most results will be available for our
Final Reporting Workshop which is planned from 2-4
December 2009 at Montego Bay, Jamaica.

You will note that we now have a logo for our CCPP
project depicting our famous white grunt, thanks to
the artistic talents of Dr. Chris Metcalfe at Trent
University.


Coordination
Interim Reporting Workshop
An Interim Reporting Workshop which was held from
21-22 January 2009 in Merida, Mexico, aimed at
providing an update on project activities, a
discussion of problems encountered so far, and
suggestions for improvement. It also provided an
opportunity to present and discuss research projects,
laboratory capacity enhancement, training and
monitoring activities that will be taking place during
2009 and plans for extending the project beyond
2009.

Partners Meeting
A Partners Meeting was also held in Merida, Mexico,
after the Interim Workshop, from 23-24 January
2009. The purpose of this meeting was to explore
the possibility of extending the project beyond this
phase. A draft concept note for a follow up phase
beyond 2009 was discussed and potential
involvement and roles of partners were explored.


A final proposal for phase 3 (2010-2014) was
developed as a follow up to this meeting and
submitted to the World Bank, which has informally
approved the project outline. The proposal has also
recently been sent to partners in the eight project
countries for their endorsement.

Representatives from UNU-INWEH and Trent
University attended the Caribbean Eco Health
Programme (CEHP) Partners Meeting in May 2009 in
Rousseau, Dominica. The objective of the CEHP
meeting was to take stock of the successes of the
programme to date as well as to determine how best
to move forward in the coming years and link with
partners. UNU-INWEH presented the CCPP status
and plans. There are clear links between the CCPP
and CEHP projects and this meeting gave a further
opportunity to define these links and decide on joint
activities. A decision was made to expand the
collection of white grunt if additional funds are
found as well as the oyster bio monitoring and
passive sampler research projects to other islands in
the CARICOM region to ensure a more regional data
set.


Capacity Building
Laboratory Equipment Upgrades
One of the outcomes of the 2008 laboratory
assessment exercise was determining the laboratory
equipment needs so that lab capacities for POPs and
PTS analysis reach an acceptable level for the
project. Due to budget constraints the initial
laboratory infrastructure investments have been
targeted at two Regional Laboratories (CINVESTAV in
Mexico and UWI lab in Jamaica). In April 2009, two
new Agilent 7890A Gas Chromatograph systems
were purchased. The GC-ECD was installed in the
UWI lab during June 2009 and is functioning well and
was used for the inter-lab comparison exercise. The
GC-ECD for Mexico is not yet installed due to delays
in attaining a radio isotope license for Mexico.

Laboratory Inter comparison


http://www.inweh.unu.edu/Coastal/POPs/POPmainpage.htm








Caribben Coatstl PoluUon Pro.ct


This exercise commenced in October 2008 and aims
at conducting an inter comparison between the two
Regional Laboratories (CINVESTAV Mexico and UWI
Jamaica) and the two Canadian laboratories
(University of Windsor and Trent University
Laboratories). This exercise will provide an initial
quality check to ensure consistency in analytical
results between labs and harmonize selection of
standards and certified reference materials (CRMs)
used for quality assurance purposes.

Tuna homogenate certified reference materials
(CRMs) from the IAEA Marine Laboratory in Monaco
were used in this exercise as well as certified
analytical standards (Organochlorine pesticides and
PCB Standards), and a spiking internal standard
(2,4,6-trichlorobiphenyl) purchased in Canada.

Each laboratory performed sample extractions,
clean-up and chemical analysis of 8 replicate samples
of the supplied CRM. Instrumental analysis of the
blanks and replicate CRM samples were completed
by GC-ECD. Electronic reports from each of the 4
participating laboratories were combined and the
results are nearly final. A presentation of results will
be given at the final reporting workshop in
December 2009. In the meantime the results will be
sent to the 4 labs and necessary action will be taken
to improve the methods and analytical outcomes.

Training

Training Workshop on Laboratory Methods and
Procedures
A Training Workshop on Laboratory Methods and
Procedures for Persistent Organic Pollutants in
Biological Tissues was held from 19-20 January 2009
at the Reef Yucatan Hotel, in Merida, Mexico. It
included sessions on QA and QC procedures,
examples of different extraction and clean-up
techniques, instrument detection limits and method
quantization limits and POPs extraction methods. A
total of 15 participants from the eight project
countries attended. Four trainers with extensive
experience in environmental POPs issues conducted
the training: Dr. Chris Metcalfe, Trent University; Dr.
Ken Drouillard, University of Windsor; Dr. Gerardo


Gold, CINVESTAV Unidad Merida, Mexico; and Ms.
Nargis Ismail, University of Windsor.
The training included a practical laboratory exercise
at the CINVESTAV laboratory. It gave participants an
opportunity to visit the laboratory and campus, and
to see some hands on demonstrations of different
extraction techniques, and practice some of these
techniques.

Training of Lab staff in Canada
Three laboratory staff, 2 from the University of West
Indies Pesticides Laboratory and 1 from the
CINVESTAV Unidad Merida Laboratory, visited the
laboratories at the University of Windsor and Trent
University for 2 weeks during March 2009.


The main focus of this South visits North training at
accredited laboratories in Canada, was to increase
the capabilities of the laboratory staff in aspects of
quality control, quality assurance and extraction, and
clean up techniques and methods for determination
of Organochlorine pesticides and PCBs in biological
tissues.

The training was coordinated by Dr. Chris Metcalfe,
Director of the Institute for Watershed Science at
Trent University and Dr. Ken Drouillard, Head of the
Organic Analytical Laboratory at the Great Lakes
Institute for Environmental Research, University of
Windsor, and their lab staff.

Instructional video on POPS extraction methods in
fish tissues for laboratories
This instructional video was prepared by Trent
University, Peterborough, Canada and demonstrates


http://www.inweh.unu.edu/Coastal/POPs/POPmainpage.htm









Caribben Costsl PoluUon Projl


a standard method for the extraction of Persistent
Organic Pollutants (POPs) from fish tissue samples.
We hope that through the use of this video, all the
laboratories involved in the Caribbean Coastal
Pollution Project (CCPP) will be able to prepare
extracts of fish tissues to send to regional
laboratories for POPs analysis. This video also
provides detailed information on the equipment
required for this procedure. This video is available
upon request: please email: vanlav@inweh.unu.edu.


White Grunt Monitoring


The prototype database is currently embedded into
a Microsoft Access 2003 DB file using Access' Visual
Basic for Applications.


Once all the fish collection data are entered centrally
by UNU-INWEH, this database will be distributed to
the two laboratories that are currently responsible
for analysis of the fish samples. The sample analysis
results will be entered by the regional laboratories,
then quality checked, processed and aggregated into
a single master DB that will reside at the IWS and/or
UNU-INWEH for the short-term.


This database will also serve to link participating labs
and encourage the concept of data sharing and data
comparison.


Sample collection and analysis


Sampling of the white grunt (Haemulon plumieri)
commenced in the months following the regional
workshop in Trinidad, in June 2008. Three fish were
collected at each of the 6-9 sites in all 8 countries.
The dorsal muscle of these fish (2-5 g, skin-off) was
then dissected and stored on ice. Nearly all samples
have now been sent to respective regional
laboratories and analysis of these samples will
hopefully start within the upcoming weeks. Annex 1
gives an update on the status of white grunt
sampling in each country for the first sampling
round.

Nearly all the countries have agreed to do a second
round of sampling during this summer of 2009. This
will provide us with a data set in time which will be
useful in identifying temporal trends.


Database
A relational database (DB) has been developed by
the Institute for Watershed Science (IWS) at Trent
University for efficient storage, retrieval,
maintenance and reporting of the white grunt
monitoring data and associated metadata.


Sample ID BZ001 00101-CIN-001
OnginalSample D BELIZE-1-1
Collectors Name JorgeHernander
CaptureMethod Hookandine
Capure Date 13/12/2008 dd/mm/yyyy
SampleStatus Processed
Storage Location Labfidg Room 756


Habitat Type
SubstrateType
PRoxlm To
Waer Depth
Water Temp
Sal,,ty


Sample Lab Rep
001 CIN 001
Species Name White Gl
Length 41 m
Weight 2100 g
PhysicalCondit i
Image Souce Ab n/couon







c ....N.........
comment*


Lagoon
se ........ ..
'NHal liver outlet
12;
22 Celsius
35 ppl


CEmeeI


Research

Passive Sampler Monitoring for Contaminants in the
Caribbean Coastal Zone of the Yucatan Peninsula,
Mexico. Principle investigator: Dr. Chris Metcalfe


The Caribbean coast of the Yucatan peninsula is
highly susceptible to groundwater and coastal zone
contamination as a result of the unique Karst
geology and the rapid development that is occurring
in this region. Flooded caves provide a hydrological
conduit that link inland recharge areas to springs
which discharge into the coastal zone and sinkholes
cenotess), providing direct conduits from the surface


http://www.inweh.unu.edu/Coastal/POPs/POPmainpage.htm








Canbb..n cobsI Podlfon Proj*


into these cave systems.


In order to evaluate contamination of groundwater
and the coastal zone in the Riviera Maya region, we
conducted a monitoring study (commenced in Dec
2008) of selected groundwater discharge zones
along the Caribbean coast between Puerto
Aventuras and Tulum. Passive samplers (both
Permeable Membrane Devices (SPMD) and Polar
Organic Chemical Integrative Sampler (POCIS)
passive samplers) were deployed for a period of one
month from early December 2008 to early January
2009 in cenotes and underwater caves at 5 sites. The
samplers were then collected and analyzed for
several different classes of contaminants. Analysis is
being performed at Trent University, the GLIER lab of
the University of Windsor, Environment Canada, and
CINVESTAV, Unidad Merida, Mexico.

Preliminary results indicate that the freshwater
resources at several locations have been
contaminated by chemicals that could only have
originated from domestic sewage. Final results will
be presented at the final reporting workshop in Dec
2009.

A workshop organized by the Amigos de Sian Ka'an
on September 1, 2009 in Cancun will provide an
opportunity for members of the study team to
present their research results to regional
stakeholders and to discuss the probable sources of
contamination. The workshop will also be an
opportunity to discuss best management practices
for reducing the likelihood of contamination of the
aquifer, such as changes to sewage disposal


~ ~i~


http://www.inweh.unu.edu/Coastal/POPs/POPmainpage.htm


practices, installation of impermeable liners, and
protection of recharge areas.

Quantitative Biomonitoring of POPs in Caribbean
Coastal Zones Using Oysters. Principle investigator:
Ken Drouillard.
This project aims to implement an oyster
biomonitoring survey and to calibrate Caribbean
oyster species as POP biomonitors for the region.
Researchers and their respective graduate students
will be trained in biomonitoring methods and
toxicokinetic modelling techniques to interpret POPs
bioavailability and bioaccumulation in each of their
respective regions. The data will also provide
calibration of chemical toxicokinetics in oysters that
will facilitate better interpretation of spatial scale
biomonitoring data sets implemented according to
classic biomonitoring approaches as well as provide
information on site specific seasonal changes in
bioavailable residues.

During the 1st year, a classic biomonitoring survey
will be implemented, where investigators will collect
native mussels from each study location and submit
them for chemical analysis (OC-pesticides, PCBs,
PAHs and PBDEs).

Surveys will be done in regional waters of Trinidad,
Jamaica and Mexico. So far, oysters have been
collected in all three countries. In Jamaica, oysters
were collected at 2 sites. At one of these, multiple
species were collected. An additional site will be
sampled soon. A student is in charge of this
sampling. In Mexico and Trinidad, oysters were
collected from 3 locations. In Barbados (additional
site), Dr. Emma Smith of UWI will provide oysters
from 3 locations.

During the 2nd year, a quantitative biomonitoring
survey will be implemented, whereby pre-dosed
mussels will be transplanted in regional waters in
each country and destructively sampled over time.

Information Dissemination


Table 1 summarizes the documents, outreach
materials, posters and reports prepared as part of









Caribbwn Costl PolluUon Projat


the project so far. More focus on outreach will be
given during the last part of 2009, including outreach
to public and policy makers.

As a way to present some results of the project, the
following posters were presented at the Ross
University Research Day Theme: Public Health in
Developing Countries: Challenges and Solutions, 22nd
May 2009 in Dominica.

* Hanneke Van Lavieren, UNU-INWEH, Enhancing
capacity to assess and manage persistent organic
pollutants in coastal areas of the Wider Caribbean
region.

* Chris Metcalfe, Trent University, Domestic
wastewater as a source of contaminants in
freshwater aquifers in the Mayan Riviera tourism
region of Mexico.

Our website is updated on a bi-weekly basis and
provides links to project documents and other
outputs.


Table 1. Project Documents
Document Date
Planning Workshop Report, Hamilton Nov. 2007 Jan 2008
Laboratory Evaluation Questionnaire Jun 2008
Consensus Statement on activities in the project Jun 2008
Sampling Protocol for White Grunt (Haemulon plumieri) July 2008
Regional Workshop Report, Trinidad June 2008 July 2008
Final Laboratory Evaluation Report Oct 2008
Lab equipment upgrade cost estimate (phase 1 & 2) Sept 2008
Caribbean Regional Analytical Labs- roles and
Nov 2008
participation in POPs analysis
First Interim Progress Report Report 1.2 Nov 2008
Training Workshop on POPs in biological tissues Report, March
Merida, Mexico 2009
Sampling Protocol for White Grunt (Haemulon plumieri)
Version 2- Preparation of muscle tissue samples April 2009
Poster: Enhancing capacity to assess and manage May 2009
persistent organic pollutants in coastal areas of the
Wider Caribbean region
Poster: Domestic wastewater as a source of May 2009
contaminants in freshwater aquifers in the Mayan
Riviera tourism region of Mexico.
Partners Meeting Report, Merida, Mexico May 2009


NEWS


At the fourth meeting of the Conference of the
Parties (COP 4) of the Stockholm Convention (SC),
meeting in Geneva from 4-9 May 2009, 160
Governments adopted amendments to include
nine new persistent organic pollutants (POPs) to
the list of the existing list of 12 SC POPs.

The nine new chemicals added to the list are:

1. Alpha hexachlorocyclohexane;
2. Beta hexachlorocyclohexane;
3. Hexabromodiphenyl ether and
heptabromodiphenyl ether;
4. Tetrabromodiphenyl ether and
pentabromodiphenyl ether;
5. Chlordecone;
6. Hexabromobiphenyl;
7. Lindane;
8. Pentachlorobenzene;
9. Perfluorooctane sulfonic acid and
Perfluorooctane sulfonyl fluoride.


http://www.inweh.unu.edu/Coastal/POPs/POPmainpage.htm











Annex 1


Samples Data
Data
Country Samples Sent to Problems sheets Sampling
Country Problems sheets
Collected Regional submitted Report
submitted
Lab?

Belize yes yes The first set of samples yes yes
from Jan 2009 were
thrown away accidentally
by others at fisheries
ministry but fish have
been resampled
Dominican Republic yes yes Samples sent by fed ex yes yes
and it took 4 days to
arrive in Merida- samples
were rotten despite ice
packs. However still
usable.
Guatemala some not yet Grunt cannot be found at no yes
4 sites. Only 2 sites
sampled so far. The
marine field station
burned down by rebels
against the government -
no boats available.
Honduras yes no Fish are collected at all yes yes
sites the problem is
sending them to Mexico
(Fed ex problems) -
probably this month (Aug)
Jamaica yes yes One site missing yes yes
Kingston harbour no fish
found
Mexico yes yes The first set of samples yes yes
from 2008 was thrown
away accidentally by
others at ECOSUR were
resampled and resent to
CINVESTAV lab
St. Lucia yes yes One site missing yes yes
Trinidad yes yes Samples were sent and yes yes
(but samples got stuck in Miami
lost need customs for a week then
to be re- resent to Trinidad rotten.
sent) Fish still in freezer so
additional muscle tissue
can be dissected planned
for Aug 09.




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