FLORIDA AGRICULTURAL EXPERIMENT STATION
Department of Dairy Science
Dairy Science Mimeo 5$-2
February 5, 1954
Preliminary Report: Relationship of Menadione to Milk
Souring and to Dairy Culture Lactic Acid Development.
H. H. Wilkowske, W. A. Krienke and L. R. Arrington
This Department received several inquiries relative to delayed milk souring
resulting from the feeding of menadione, soon after Kelley and Dittmer, Florida
State University at Tallahassee, announced their results. Some of the requests for
additional information were concerned specifically with the possible effects on
lactic acid production by dairy starters used in the manufacture of fermented
dairy products. A study was undertaken to supply the needed answers and this
preliminary report is a brief summary of the results to date.
When four cows of the Department of Dairy Science dairy herd were fed
25 mg. menadione per day (mixed into the grain ration of the evening feeding) for
a period of 28 days, no change was observed in the rate of souring brought about
by the normal bacterial flora of the milk. In second portions of the milk samples,
inoculated with various commercial buttermilk and cheese cultures, no change was
noted in rate of acid development which could be attributed to feeding the menadione
Results were similar when two other cows were fed the menadione at the rate of
100 mg. daily for 20 days. Another group of four cows, on trials still in progress,
have received 300 mg. of menadione twice daily for 20 days and the results have
been similar to those of the previous studies.
Under feeding conditions as practiced in these studies the feeding of
menadione did not affect the normal rate of milk souring nor the rate of lactic
acid development by dairy cultures.