Citation
Summer Focus on the Physical Sciences and Quantitative Research: A Novel Approach for Actin Bundle Identification and Analysis in Fluorescence Microscopy Images

Material Information

Title:
Summer Focus on the Physical Sciences and Quantitative Research: A Novel Approach for Actin Bundle Identification and Analysis in Fluorescence Microscopy Images
Series Title:
Journal of Undergraduate Research
Creator:
Hamed, Malek L.
Lan, Tian
Tseng, Yiider
Place of Publication:
Gainesville, Fla.
Publisher:
University of Florida
Publication Date:
Language:
English

Subjects

Genre:
serial ( sobekcm )

Notes

Abstract:
Analysis of filamentous actin structures in microscope images is a frequent step in cell research. This analysis is commonly qualitative and based on human inspection, limiting accuracy and quantitative import. Current alternatives involve specialized equipment, require high-resolution images, or limit the structural information that can be extracted. Here, a new approach is developed and applied to wide-field fluorescence microscopy images of actin-stained cells. This analysis was successfully used to extract data regarding individual filament bundles and produce distributional information. A few weaknesses are noted, including occasional erroneous segmentation of intersecting filament bundles and poor results for high-noise images. Future work should investigate the relationships between extracted structural information and physical parameters of filament bundles to strengthen and broaden inference potential.

Record Information

Source Institution:
University of Florida
Holding Location:
University of Florida
Rights Management:
Copyright by Creator or Publisher. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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Full Text

PAGE 1

College of Engineering, University of Florida

PAGE 2

ratio of variations mean directional profile r, r0 r90 orientation matrix Identification of Filament Subdivisions

PAGE 3

Rejoining of Detached Segment Groups Filament Bundle Analysis Orientation Length Diameter. Cell Preparation and Microscopy

PAGE 4

Filament Identification Distributional and holistic analysis