Summer Focus on the Physical Sciences and Quantitative Research: A Conservation Genetic Analysis of the Endangered Florida Bonneted Bat

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Summer Focus on the Physical Sciences and Quantitative Research: A Conservation Genetic Analysis of the Endangered Florida Bonneted Bat
Series Title:
Journal of Undergraduate Research
Alvarez, Valentina
Gore, Jeff A.
Pylant, Courtney
Austin, James D.
Place of Publication:
Gainesville, Fla.
University of Florida
Publication Date:


serial ( sobekcm )


The Florida bonneted bat is one of the rarest bat species in North America, and has been designated as federally endangered since 2013. Very little is known about the basic biology of Florida bonneted bats, and this project was initiated to fill some important gaps regarding the social structure, genetic diversity, and mating system of bonneted bats located at 12 artificial roosting colonies in Charlotte County, Florida. This location represents the largest known population of the species in Florida. Samples were collected over a 32-month period (April 2014-December 2016), during nine tri-annual trapping sessions by Florida Fish and Wildlife Conservation Commission biologists and volunteers. Wing punches were collected from all unique bats to allow for estimation of genetic diversity and molecular relatedness among bats to answer questions of the species’ mating system. The objective of this paper was to test whether roosts within the WMA display patterns of isolation by distance and to determine if relatedness among females is greater than among males within roosts. Genetic distance calculated from 13 microsatellite loci revealed a pattern of significant isolation by distance among roosts spanning approximately 16 kms. Relatedness within roosts was greater among females within roosts than among males, reflecting a pattern driven by male-biased dispersal, which is in line with the expectation of a polygynous mating system. These results provide an important initial genetic picture of the diversity and mating system of Florida bonneted bats.

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University of Florida
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University of Florida
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1 College of Agriculture and Life Sc iences, University of Florida 2 Florida Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission (Eumops floridanus) E. floridanus E. glaucinus E. floridanus E. floridanus E. floridanus E. floridanus


Sample Collection Microsatellite Primer Development DNA Extraction and PCR Genotyping and Data Quality


Analysis of Population Substructure K K K K K K K Genetic Relatedness Analysis Locus Variability Population Substructure K K K K K


Isolation by Distance Intra-Roost Kinship Results


E. floridanus E. floridanus, Molecular Ecology 10 Proceedings of the Royal Society of London B: Biological Sciences 266 Biometrics Conservation genetics resources 4 Econometrica: Journal of the Econometric Society


Molecular ecology 14 Molecular ecology notes 2 Ecology 79 Journal of biomolecular techniques: JBT 22 Reproductive biology of bats 12 Heredity 115 Pathology & Oncology Research 4 Molecular ecology notes 6 The American Naturalist 154 Trends in Ecology & Evolution 8 Evolution Journal of heredity 86 Genetical Research 67 Bioinformatics methods and protocols Journal of Mammalogy 85 Molecular Ecology Notes 4