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Signal Transduction: Environmental Stimulus to Changes in Global Transcription in Porphyromonas gingivalis
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 Material Information
Title: Signal Transduction: Environmental Stimulus to Changes in Global Transcription in Porphyromonas gingivalis
Series Title: Journal of Undergraduate Research
Physical Description: Serial
Language: English
Creator: Smith, Corey James
Progulske-Fox, Ann ( Mentor )
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: 2009
Abstract: Porphyromonas gingivalis is an anaerobic oral pathogen that has been associated with atherosclerosis and coronary heart disease. Genetic regulation in eubacteria occurs primarily at the level of transcription. The specificity of RNA polymerase for promoters is determined by the sigma subunit. Regulation of sigma factors is generally achieved by an attached proprotein sequence or an anti-sigma factor capable of responding to environmental cues. The string database was used to determine the genes in the operons containing putative sigma factors in the P. gingivalis genome. The putative sigma factors were tested for proprotein sequences using signalP peptide cleavage site prediction software. This test concluded that none of the putative sigma factors contained a cleavage site similar to any previously characterized system. The genes in cotranscriptional units with sigma factors were tested for transmembrane helicies using TMHMM, revealing that seven of the ten operons containing a sigma factor had an integral protein. These results provide a set of possible environmentally reactive transcriptional regulators that can be confirmed with a yeast two-hybrid test to confirm protein-protein and protein-DNA interactions.
 Record Information
Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
System ID: UF00091523:00577

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