Immunohistochemical Localization of the Red Tide Organism (Karenia brevis) in the Florida Manatee (Trichechus manatus la...
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Title: Immunohistochemical Localization of the Red Tide Organism (Karenia brevis) in the Florida Manatee (Trichechus manatus latirostris)
Series Title: Journal of Undergraduate Research
Physical Description: Serial
Language: English
Creator: Flandermeyer, Liesl K.
Samuelson, Don A.
McGuire, Peter
Lewis, Patricia A.
Publisher: University of Florida
Place of Publication: Gainesville, Fla.
Publication Date: Fall 2008
Abstract: The female Florida manatee (Trichechus manatus latirostris) only produces a single calf every 2.5 to 5 years. The current population of manatees is 3,000 with an annual death rate of 400. Consequently, the manatee population may continue to decline until it reaches extinction. One leading factor that decreases the manatee population is the exposure to Florida red tide brevetoxins, produced by the dinoflagellate organism Karenia brevis. Sections were made from formalin-preserved paraffin-embedded blocks of manatee tissue. Following incubation with the primary K. brevis antibody overnight as well as no antibody as a negative control, the tissue sections were incubated with biotinylated donkey anti-sheep IgG, streptavidin ABC-AP and chromagen AEC for 10 minutes each. The tissue slides were then examined for traces of K. brevis, which appeared red on the sections. The organism was concentrated in the mandibular, axillary, retropharyngeal, and T-septal lymph nodes, as well as elements of the mucosal-associated lymphoid tissues (MALT). By successfully developing an immunohistochemical test to determine the presence of K. brevis in manatee lymphoid tissues, the pathogenesis of brevetoxin and its mode of entrance into the body can be better understood, revealing the impact of red tide on the survival of the Florida manatee.
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Source Institution: University of Florida
Holding Location: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
System ID: UF00091523:00002

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