LA Soi an Wae Scec Deatmn Publ--icatio
Volume 6 Number 2 Institute of Food and Anricultural Sciences
& Soil Moisture Labs
and Mineralogy Lab
Stable Isotope M.ass
Spec trometry Lab
IIIR Spectrometer Lab
ARL & ESTL Labs
GIS & Soil Microbiology
Research and Education
Our Outstanding Students
Faculty a Staff rle.'s
Dr. Vimala Nair
UF UNIVERSITY of
FROM THE CHAIR
The Soil and Water Science Department (SWSD) faculty and students use a wide range of
analytical and field instrumentation in their research programs. To maximize the use of these
analytical instruments, groups of faculty established several core laboratories in the
department with the objective of sharing instrumentation and avoiding duplication of facilities.
The instrumentation in each core laboratory was purchased by select faculty through their
grants and contracts with some matching support from state funds. Instrumentation in these
laboratories are primarily used by these faculty and by their graduate students. In addition, at
a minimal cost, the instrumentation in each of these core laboratories are now made available
to other SWSD faculty and to those outside the department. Details of costs associated with
the use of these laboratories can be obtained from the coordinator of each laboratory. A brief
description of the core laboratories is presented in this newsletter.
Here are few highlights since the last newsletter:
Steve Bloom, Senior Chemist retired after 23 years of dedicated service with the department.
For the past four years, Steve worked in the GIS Research Laboratory. Prior to this assignment,
he worked with the Soil Physics group. We thank Steve for his outstanding service to the
L. Rex Ellis has been appointed as an Assistant Research Scientist in the Environmental
Pedology program. Rex will conduct research on subaqueous soils.
Todd Osborne has been appointed as an Assistant Research Scientist in the Wetlands program.
Todd will conduct research on biogeochemical cycling of nutrients in wetlands and aquatic
-' Continued on Page 7
DrK Raes Reddy Chir Soi an Wae Scec Departmnt 106. Neel Hal Box 1150 Unvrst of Flria Gansvle Flor0ida
321. Telephon 329210;g Fa 329-39..Eal k f.ed htp:/sil*iasuf.ed
CORE LABORATORIES PAGE 2
Soil Chemistry, Environmental Hydrology Et Soil Moisture Labs
Soil Chemistry Laboratory
The Soil Chemistry Laboratory was established to provide basic soil chemical -
analyses to students and faculty in the SWSD. Instrumentation consists of a Varian ,
Fast Sequential Atomic Absorption Spectrophotometer, model 220FS and a High
performance liquid chromatograph (HPLC) (Waters 2690 Separations Module, Waters
2487 Dual Wavelength Detector, and a Waters 432 Conductivity Detector). The Soil
Chemistry Laboratory is primarily a teaching facility and, therefore, students are
given priority in use of this facility. Users are trained to use the instruments and
associated software and are expected to prepare, process, run, and analyze their
own samples. Users provide their own standards and QA/QC samples. Users must
also provide their own HPLC columns and mobile phases. For additional -
information and fees for use of instrumentation, contact Dean Rhue at Bill Reve loads a sample into the HPLC
firstname.lastname@example.org. to test for organic acids.
Environmental Hydrology Laboratory
The Environmental Hydrology Laboratory provides specialized laboratory
equipment including a Perkin Elmer gas chromatograph, Dionex accelerated
7 solvent extractor, and an Ocean Optics fiber optic spectrophotometer.
Specialized field equipment includes pressure transducers, autosamplers,
and a Sontek acoustic doppler velocimeter. Services available include
equipment usage, field hydrology assistance, and modeling of contaminant
transport in soils and sediments. For additional information, visit http//
hydrology. ifas.ufl.edu or contact Jim Jawitz at email@example.com.
Huaguo Wang uses visualization research for
quantifying DNAPL dynamics.
Soil Moisture Laboratory
The Soil Moisture Laboratory is located in McCarty Hall and offers procedures for
determining soil moisture release curves, saturated hydraulic conductivity and bulk
density on individual samples. Specific equipment and soil cores required for soil sample
collection for moisture release procedure can be borrowed by contacting the Lab at 352-
392-1951 x223. Additionally, services to determine particle size distribution on a separate
soil sample are also available. For additional information, contact Rao Mylavarapu at
firstname.lastname@example.org. Kelly Nielson connects a tempe
cell to pressure lines to determine
Environmental Pedology and Mineralogy Laboratory
The Pedology-Mineralogy Laboratory has personnel with expertise who perform
physical, chemical, and mineralogical characterization of soils. Much of the
data generated by the Laboratory is pertinent to soil genesis, classification,
and management. Routine analyses include particle size, organic carbon,
extractable metals, cation exchange capacity, pH, electrical conductivity, total
phosphorus, and mineralogy. Major instrumentation within or associated with
the Laboratory includes an x-ray diffractometer, thermogravimetric analyzer,
differential scanning calorimeter, and ground-penetrating radar system. A list
of services provided by the Pedology-Mineralogy Core Laboratory can be found
at http://soils.ifas. uft.edu/research/pedology. pdf.
Rocky Cao is operating the x-ray
diffractometer for determining mineral For additional information, contact Willie Harris at email@example.com.
change in soil with amendments.
SStable Isotope Mass Spectrometry Laboratory
The Stable Isotope Mass Spectrometry Laboratory (SIMSL)
located in the Soil and Water Science Department.
utilizes a Thermo Finnigan MAT Delta Plus XL mass
spectrometer designed to cover a mass range up to 70
amu to determine the stable isotope ratios of certain
gaseous elements. Since most elements of biological
interest including C. H. O. N. and S have t' ,o or more
stable isotopes '. which fall ..'ithin this range the Delta Plus
XL is '. ell suited for determining the stable isotope ratio
for these elements. The stable isotope ratios of C t N
are routinely determined by Elemental Analyzer Mass
Spectrometry in this facility from plant and soil samples
submitted for analysis. Results are reported as Atom
Atom excess, or Delta o/oo units. Three peripherals
(elemental analyzer, gas chromatograph. and gas bench
are presently dedicated to the mass spectrometer for the
purpose of converting the sample from a solid or liquid
state to a gaseous state prior to introduction into the
mass spectrometer. The SIMSL is under the supervision
of the Soil and Water Science Department: cooperating
departments are the departments of Geological Sciences
and Botany. For additional information on using this
facility, contact Don Graetz at dagifas.ufl.edu.
Patrick Inglett analyzing carbon at the Stable
Isotope Mass Spec Lab.
NIR Spectrometer Laboratory
The NIR Spectrometer
Laboratory is available
requiring scanning of
samples in the Near
Infrared region (350-
2800 nm). For
and fees for instrument
use, contact Camilo
ccordavl uft.edu or Rao Camilo Cornejo scanning
Mylavarapu at samples through FieldPro NIR
ARL a ESTL Labs
Mary MacLeod, analyzes samples for nitrate
plus nitrite by semi-automated colorimetry.
Analytical Research Laboratory
The UF/IFAS Analytical Research Laboratory (ARL) offers
high quality analytical services to all UF/IFAS researchers
state-wide. Researchers are requested to contact the Lab
a few months in advance regarding their analytical needs
and for scheduling analyses. Most of the analyses are
certified by National Environmental Laboratory
Accreditation Program (NELAP), E72850. All information
on analytical services, available schedules and sample
submission forms are available at http://arl.ifas.ufl.edu/
or by contacting ARL at firstname.lastname@example.org.
Extension Soil Testing Laboratory
The UF/IFAS Extension Soil Testing Laboratory (ESTL)
offers a variety of diagnostic tests for mineral soils,
container media, tissue (blueberry and pecan), pine
nursery and irrigation water. Test results will include
recommendations and general guidelines for time and
nutrient applications and for improving irrigation water
quality. These services are available to all Florida
residents for a fee. Information about the available
tests, fee schedule and free sample boxes/bags can be
obtained by contacting ESTL at email@example.com or by
contacting your local County Extension Office. Additional
information can be found at http://soilslab.ifas.ufl.edu/.
Maintenance of instrumentation insures that our
clients receive quality data. Hui X Lu working with one
of the lab's ICP instruments for metals analysis.
GIS Research et Soil Microbiology Labs
I .GIS Research Laboratory
The GIS Research Laboratory provides professional services on the use
of geographic information systems, geostatistical and statistical
methods, remote and soil sensing (e.g., visible/near-infrared diffuse
reflectance spectroscopy). The GIS laboratory is a modern computer
laboratory providing hardware and software including multiple data
servers, 16 PCs with flatscreen monitors, large format color printers,
and scanners. Available software packages include: MS Office Suite;
MS SQL database; ArcGIS Suite (ESRI) including Spatial Analyst and 3D
Analyst; Idrisi Kilimanjaro GIS; Erdas Imagine for remote sensing
analysis; SGeMS, SAS, GenStat and SPSSWin for geostatistical and
j statistical analyses; Adobe Photoshop; Adobe Acrobat; CART -
Classification and Regression Trees; Treenet; Neurosolutions for
Jinseok Hong models soil properties neural network modeling; DreamWeaver; Unscrambler for
using GIS and Remote Sensing. chemometric modeling; Environmental Visualization Software (EVS-
PRO), and RockWorks for 3D modeling and scientific visualization. A
sub-meter accuracy, differential GPS unit (Trimble Pathfinder) is available for geo-referencing of sampling
locations. In addition, several handheld GPS units are available for field navigation. For additional information,
contact Sabine Grunwald at firstname.lastname@example.org.
Soil Microbiology Laboratory
The Soil Microbiology Laboratory (SML) has a wide array of equipment and !
services available for analysis of the biological component of soils. The
primary areas of expertise are characterization of nucleic acids, from
extraction from soil samples through phylogenetic analysis, enumeration of
functional groups by most probable number analysis, and development and
characterization of endomycorrhizal fungi inocula, including assessment of ,
mycorrhizal inoculum potential and development of commercial scale
innocula. The SML also conducts short courses on isolation and
characterization of mycorrhizal fungi each summer. A full listing of services ..
and fees may be found at http://molecol.ifas.ufl.edu/core.html. For
additional information, contact Andy Ogram at email@example.com. Abid Al-Agely determining
-i11_ I I .. #t #; I
Wetland Biogeochemistry Laboratory
Yu Wang, Dawn Lucas, and Gavin Wilson discuss
results of an Ortho P test run on the AutoAnalyzer 3.
The Wetland Biogeochemistry Laboratory is a NELAC certified
facility (certification # E72949) in the category of General
Chemistry for water and soil samples. The lab is equipped with
four flow analyzers for nitrogen and phosphorus analysis, a new
discrete analyzer, four gas chromatographs, ion chromatograph
(Dionex), CNS analyzer, dissolved carbon analyzer, and other
routine instruments such as spectrophotometer, centrifuge, Eh/pH
controllers, EC meters, and incubators. The laboratory provides
specialized services (analytical and experimental) to our
collaborators working on joint projects.
For additional information, contact Ramesh Reddy at firstname.lastname@example.org
or visit http: //wetlands.ifas. ufl.edu.
mycorr za nocuum p e
Research and Education Center Labs
Soil and Water Laboratory at the Tropical Research and Education Center, Homestead
The Soil and Water Laboratory (SWL) at the Tropical Research and
Education Center in Homestead is certified by the National
Environmental Accreditation Conference (NELAC). The lab employs
three full-time, well-trained lab technicians and is equipped with state-
of-the-art instruments. These instruments include an Atomic
Absorption Spectrophotometer (Shimadzu AA-6300 with GFA-EX7i), an
Autoanalyzer (Bran and Luebbe AutoAnalyzer III), a CNS analyzer
(Elementar Vario MAX), an Ion Chromatograph (Dionex ICS-2500), and
an Auto-digestion block (Environmental Express, SC860). The SWL
provides services only to their own research team and collaborators.
The SWL is interested in collaborating with other researchers on
research and extension projects related to water quality, soil
chemistry, nutrient and waste management, ecosystem restoration,
etc. For further information visit http://yuncong.ifas.ufl.edu or
contact Yuncong Li at email@example.com.
contact Yuncong Li at un fas.uf.edu. In-service training for state and county faculty
on analyzing soil and water samples.
Soil and Water Science Laboratory at the Indian River Research Education Center, Ft. Pierce
Qin Lu operating the
The Soil and Water Science Laboratory (SWSL) at the Indian River Research
Education Center (IRREC) in Fort Pierce is a self-supporting laboratory. Its mission is
to provide legally defensible and scientifically credible analytical and technical
support to the Research and Education Programs in Soil and Water Science at the
IRREC. The laboratory is a certified NELAC standard lab for environmental tests in
the category of General Chemistry and Trace Metals (NELAC certification # E76888).
It is equipped with inductively coupled plasma atomic emission spectrometry
(ICPAES, Ultima, JY Horiba, NJ), CN Analyzer (Vario Max, Elementar, Germany), N/P
Discrete Analyzer (EasyChem Plus, Systea Scientific, Inc., Italy), Ion Chromatograph
(DX 500, Dionex, CA), Root Scanner (WIN/MAC-LA1600), and other routine
instruments such as UV-Spectrometry, centrifuge and lon/Eh/EC meters, and
incubators. The SWSL is currently not certified as a commercial lab and therefore, is
\ not qualified to provide analytical services to outside clients. However, the
laboratory provides services to our collaborators working on joint projects. For
additional information, contact Zhenli He at firstname.lastname@example.org.
Everglades Research and Education Center Laboratories, Belle Glade
The Water Resources Group at the Everglades Research and Education
Center (EREC) has established a NELAC certified lab (certification #
E76463) in the category of General Chemistry. The lab is equipped with
two flow analyzers for phosphorus analysis (LACHAT and ALPKEM), a new
discrete analyzer (SEAL) mainly used for nitrogen analysis, ion
chromatograph (Dionex) and other routine instruments such as
spectrophotometer, centrifuge and EC meters, and incubators. The
laboratory provides services to our collaborators working on joint projects.
The EREC also hosts a Soil Testing Laboratory (ESTL) serving clients
throughout the Everglades Agricultural Area by doing routine tests on
organic and sandy soils including pH, P, K, Si, specific conductance and
trace elements, and providing fertilizer recommendations. In addition to
routine instruments, the laboratory has an Atomic Absorption
Spectrometry, and an Inductively Coupled Plasma Atomic Emission
Spectrometry. For additional information on both laboratories, contact
Samira Daroub at sdaroub@ifas. ufl.edu.
Viviana Nadal (right) with Samira Daroub
analyzing water samples.
I I I I
-FACULTY, STAFF E STUDENTS
Our Outstanding Students
New Graduate Students
Alex Cheesman. Advisor. K. Reddy
Jinghua Fan. advisor. Z. He & L. Ma
Yandi Fan, Advisor. R. MAyla-arpu
Hiral Gohil. Advisor. H. Ogram
Tae-Goo Oh, Adv.isor. M. Clark
Anhui Huang, Advisor. M. Teplitski
Ying Liu. Advisor. G. O'Connor
Shiny Mathe -'s, Advisor, L. Ma
Jing Nie. Advisor. M. Teplitski
Julie Pado' ,ski. Advisor. J. Ja' .itz
Rajendra Paudel. Advisor. J. Ja -'itz
Priyanka Roy. Advisor. C. Macko.tiak
Rongzhong Ye. Advisor. A. Wright
Subodh Acharya, Advisor, R. Mylavarapu
Jay Bost. Advisor. H. Popenoe
D' ain J. Butler. Advisor, M. Teplitski
Julie Driscoll. Advisor. D. Graetz
Robin Ferguson. Advisor. K. Reddy
William Grant. Advisor. M. Clark
Jason Hood. Advisor. M. Clark
Italo Lenta. Advisor. M. Clark
Louis Mantini. Advisor. K. Reddy
Stephen McCullers. Advisor. M. Clark
Frank W. Meado' sAd.isor. K. Morgan
Matthe Miller. Advisor. G. O'Connor
Jason Neumann. Advisor. M. Clark
Regina Price. Advisor. L. Ellis
John Ro' 'land, Advisor, G. Snyder
Elisabeth Solchik. Advisor. K. Reddy
Veronica Villasenor. Advisor, R. Mylavarapu
Justin Vogel. Advisor, M. Cohen
Laura Waldo. AHd.isor. A. Schumann
Gabriel Kasozi, graduate student in the soil physics
program (Peter Nkedi-Kizza, major advisor) was awarded
the Scarborough Insurance Award by the International
Student Center, University of Florida.
Isabela Torres, graduate student in wetlands program (K.
R. Reddy, major advisor) won the best poster award at the
2006 annual meetings of the American Society of
Limnology and Oceanography, Vancouver, Canada.
Olawale Oladeji (George O'Connor, major advisor) was
awarded the fist place and Manohardeep Josan (Vimala
Nair, major advisor), the second place in the oral
presentation competition at the 2006 Joint Meeting of
the Florida State Horticultural Society and the Soil and
Crop Science Society of Florida. The third place was
awarded jointly to Carolina Medina (Tom Obreza, major
advisor) and TJ Rew (Don Graetz, major advisor).
Julie Padowski and Melissa Martin, two SWSD graduate
students, spent this summer in southern Africa
participating in a six week-long IGERT course titled
Adaptive Management Field Methods. This course
introduced the social, political, and scientific concerns
surrounding the management of the Okavango River Delta,
Botswana and neighboring watersheds. As Ph.D. fellows in
the NSF funded IGERT program on Adaptive Management of
Wetlands and Watersheds, Julie and Melissa will take
coursework and develop projects that focus on issues
impacting water quality in Florida, Africa, South America,
Mexico, and other international sites.
Congratulations... Summer 2006
Graduates-Master of Science
Jeanna Ragsdale, Advisor. M. Clark
Jason Smith, Advisor, A. Ogram
Jeffrey Smith. Ad.isor. M. Clark
PAGE 7 FACULTY, STAFF Et STUDENTS
[ Faculty Et Staff News
Continued from Page 1
P. K. Nair, Distinguished Affiliate Professor, has been selected to receive the 2006 Humboldt Research Award of the
Alexander von Humboldt Foundation, Bonn, Germany. The award, known as the Humboldt Prize, is Germany's highest
recognition for international senior scientists and carries a cash award of about $60,000. Congratulations to P. K. for
winning this prestigious recognition.
Several SWS faculty taught short courses during this summer: Hydric Soils (Wade Hurt); Soil Microbiology-Mycorrhizal Fungi
(Andy Ogram, Krishna Sundari, and Abid Al-Agely); Modern Methods for Detection of Water- and Soil-borne Pathogens
(Max Teplitski); and Geostatistical Analysis of Environmental Data (Pierre Goovaerts). These courses were organized by
the University of Florida/IFAS Office of Conferences Et Institutes (OCI) http://conference.ifas.ufl.edu/soils/index.html. We
thank OCI for the excellent work in organizing these courses.
Lena Ma, Zhenli He, and Ramesh Reddy traveled to China and presented several seminars at various universities and
research institutes. The purpose of these visits was to discuss collaborative research and educational opportunities
between UF and Chinese universities.
Wendy Graham, Sabine Grunwald, Rao Mylavarapu, and Ramesh Reddy traveled to Hyderabad-India to discuss
collaborative research and educational opportunities. During their visit Graham, Grunwald, and Reddy visited the
International Semiarid Crops Research Institute and Acharya N.G. Ranga Agricultural University and presented seminars.
Rao presented a seminar at the Jawaharlal Nehru Technological University.
Sabine Grunwald taught a short course on GIS applications in land resource management at the International Crops
Research Institute for Semiarid Tropics (ICRISAT), Hyderabad, India during July 31 August 4, 2006. The course was
attended by land resource professionals from several Indian universities, state and federal agencies. The course was joint
effort between the UF and ICRISAT as a part of the MOU between the two organizations.
Amy Shober joined the SWSD as an Assistant Professor of Landscape Nutrient/Runoff
Management at the Gulf Coast Research and Education Center, Balm, Florida. Amy received
a B.S. (1998) in Environmental Science and a B.A. (1998) in Chemistry from Virginia Tech, an
M.S. (2002) in Soil Science from Penn State University, and a Ph.D. (2006) in Environmental
Soil Management from the University of Delaware. Amy's Ph.D. research focused on
understanding the solubility of P in municipal biosolids (sewage sludge) or animal manures to
assess the risk of phosphorus loss to surface and ground water when these materials are land
applied. She has published in refereed journals and presented her research at regional,
national, and international meetings and conferences. In 2005, Amy was awarded the J.
Fielding Reed Potash and Phosphate Institute Fellowship and the Hazel Burgett Endowed PEO
(Philanthropic Education Organization) Scholar award. In 2006, she was named as a
Presidential Management Fellowship finalist. Amy is a member of the Soil Science Society of
America (SSSA), the American Society of Agronomy (ASA), Phi Beta Kappa, the Golden Key
International Honor Society, and Alpha Zeta. Outside of work, Amy enjoys swimming,
walking, kayaking, and crocheting. Amy can be reached at email@example.com.
Maria Lucia Silveira joined our department as an Assistant Professor at the Range Cattle
REC, Ona, Florida. Maria, a soil scientist, earned her Ph.D. in Soil Chemistry at University
of Sao Paulo, Brazil and University of California, Riverside. Her research program will focus
on soil fertility and environmental nutrient management practices for sustainable forage
production. Research objectives will address critical nutrient and water quality issues
related to beef cattle production in South Florida. She will also work closely with IFAS
extension faculty to promote BMPs for beef and dairy operations. Maria can be reached at
William G. Blue, 82, Emeritus Professor of Soil and Water Science
Department at the University of Florida, died in Gainesville, Florida on
July 12, 2006.
He was born in Poplar Bluff, Missouri on November 7, 1923. He enrolled in
the University of Missouri in 1941. During World War II, he served as a
reconnaissance sergeant in the 78th Infantry Division in Europe and was
awarded a Purple Heart. After the end of war, he went back to the
University of Missouri, and received his B.S. in agriculture in 1947 and
i advanced degrees in Soil Science, M.S. in 1948, and Ph.D. in 1950.
Dr. Blue was appointed as an Assistant Biochemist in 1950, by the Florida
Agricultural Experiment Station at the University of Florida, working on
soil fertility problems in forage production. Later, his research expanded
to other areas, including the characterization of the action of anhydrous
ammonia in coarse textured soils and its relation to soil chemistry and
microbiology as well as plant response. He also studied the nitrogen
efficiency of forage plants in poorly drained coarse-textured soils and
potassium and phosphorus effects in coarse-textured and highly weathered
soils. In 1964, whole agricultural programs at the University of Florida were reorganized and the Institute of
Food and Agricultural Sciences was established. The College of Agriculture, Agricultural Experiment Station, and
the Agricultural Extension Service became parts of the Institute. The faculty and their titles in these units were
also integrated. Dr. Blue, then a professor, was asked in 1968 to teach a graduate course in soil fertility. From
that year to his retirement in 1989, he had taught a total of approximately 400 students. He also served as the
advisory committee chair for 17 Ph.D. and 15 M.S. candidates in the Soil and Water Science Department. Many
of them came from overseas, including Asia, South America, and Africa. He was also a member of another 80
graduate committees in Soil Science and other departments in the Institute. He published extensively and
authored and coauthored approximately 200 scientific papers. He was deeply involved in University
international agricultural programs. He was in Costa Rica from 1958 to 1960 as a pasture agronomist and in
1985, he had a two-year assignment as a research and extension advisor in Cameroon, Africa. He also had
several short oversea assignments.
He was a member of ASA and SSSA for over 40 years and was elected a Fellow of both societies. He served as
Associate Editor for the SSSA Journal. He was a member of Sigma Xi, Alpha Zeta, Gamma Sigma Delta, and
Alpha Gamma Rho. He was a member of the Florida Soil and Crop Science Society of which he was president in
1961 and editor of its proceedings.
He retired from the University of Florida in 1989, after 39 years of a successful and outstanding career. He was
respected by his colleagues and loved by his students and those who worked under him. He is survived by his
wife of 59 years, Bernice, and three daughters, Carol Blue Garcia, Jane Blue Marquez, and Cynthia Blue Russell,
as well as seven grandchildren.
Join us at ..
The 7th Annual Soil and Water Science Research Forum
The 7th Annual Soil and Water Science Research Forum http://soils.lfas.ufL.edu/forum/l is scheduled for
September 15, 2006. in Gainesville. Florida. The forum is designed to bring together representatives from state
and federal agencies as 'ell as private industry, faculty and graduate students, and prospective students interested
in soil and ,ater science. The forum i. ll provide an opportunity for all those interested in soil and ater science
to interact ,'ith our students, faculty, and administrators on campus. We look for, ard to your participation in the
forum. If you are planning to attend, please register at http://soils.ifas.ufl.edu/forum/. For additional
information, contact Lena Ma at Iqma@(ufl.edu.