Title: Citrus leaves
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Title: Citrus leaves
Series Title: Citrus leaves
Physical Description: Serial
Creator: Citrus Research and Education Center, Institute of Food and Agricultural Sciences
Publisher: Citrus Research and Education Center
Publication Date: September 2006
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Bibliographic ID: UF00087049
Volume ID: VID00069
Source Institution: University of Florida
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Forty-Fifth Annual

Citrus Packinghouse Day


Dr. Harold W. Browning,
Center Director
UF/IFAS Citrus Research
and Education Center
700 Experiment Station Rd
Lake Alfred, FL
33850-2299
Tel. (863) 956-1151
Fax (863) 956-4631

Current links available on
the CREC home page.

For current manuscripts
submitted for publication
go to: http://www.crec.ifas.ufl.
edu/publications/faculty.htm

For news from the Business
Office go to: ',iij- ..
C. ,1 ,l, rvices/busi-
.. .. ... ,,,,,

For Citrus Leaves archives
and current online issue go
to: 1 ll, ..'.... ,. ,,|i
edu/publications/citrusleaves/
index.htm

For current "for sale" publi-
cations: Iiiil .... I.,,,
,,|i e,1,, |.,, ,.N ,, ..... .,,i, ,.i. l
sale.htm

Citrus Leaves is the monthly
newsletter for employees
and friends of CREC.

Citrus Leaves welcomes
your contributions, sugges-
tions, and corrections.

Editor/writer: Katherine
Snyder; E-mail: snyderkm@
ufl.edu, ext. 1403;
Photography/Graphics:
Gretchen Baut; Events
BHG: Linda Murphy;
Customer Service: Kathy
Witherington and Nancy
Burke; Distribution: Barbara
Thompson and Karla Flynn


undaunted by the renovations and repairs taking
place at the Ben Hill Griffin Hall, Dr. MarkRitenour
(Indian River REC), Program Coordinator, opened the
meeting of the 45th Annual Citrus Packinghouse Day.
Approximately 150 attendees were welcomed by Dr.
Harold Browning (CREC Center Director), who jokingly
referenced the recent crop estimates by saying, "No, those
are not oranges dropping on the roof," as roof repairs
continued at Ben Hill Griffin Hall.
This year's meeting featured citrus canker information
and shipping fruit under the new regulations. The
opening presentation, "Shipping Fresh Citrus Under
the New Canker Quarantine Regulations" by Mr. Mark
Estes of the Florida Department of Agriculture and
Consumer Services (FDACS), explained some of the new

(continued on page 3)


Dr. Harold W. Browning welcomes everyone
to Packinghouse Day


Lab Highlight September 2006
Dr. Ed Etxeberria's Lab
I/ n this laboratory, we concentrate our efforts to understand the fundamental physiological
Land cellular aspects of sugar and acid metabolism in sink tissues such as Citrus fruits,"states
Dr. Ed Etxeberria, Professor of Horticultural Sciences, specializing in plant cell physiology.
Continuing, Etxeberria explains, "Primary emphasis is placed into the study of the mechanisms
of sucrose and citrate uptake into the vacuole of heterotrophic plant cells and their mobilization
at times of high energy demands. At the plant level, my laboratory is also involved in the study
of the effect of imposed water deficit periods on Citrus fruit quality."
Few processes are as vital to '-
plant productivity as the uptake of
photoassimilates into heterotrophic
plant cells. Whether intended for
short- or long-term storage, uptake of
photoassimilates not only supplies energy '
and building blocks to sustain growth and
development, but excess carbon is stored
in diverse cellular structures building
massive reserves. These same reserves,
contained in the vacuole(s), amyloplasts
or elaioplasts, become sources of energy
to support metabolism during postharvest
shelf life and to support germination and
sprouting of seeds, tubers, hypocotyls, and


Etxeberria checking equipment readings.
photo by Gretchen Baut


(continued on page 3)







Human Resources
with Dale Price


Update your address for Open
Enrollment
n preparation for open eniillm innt
the state of Florida usually captures
address information in early August;
therefore, it's important to make sure
your address is current so that you
do not miss any important mailings.
To ensure you receive important P b
benefit and human-resource related
communications particularly relating to fall benefits open
enrollment make sure your home and work addresses
are up-to-date as soon as possible in the myUFL systems by
completing the following steps:
Log on to the myUFL portal with your GatorLink username
and password. (Call 392-HELP if you don't know your
GatorLink usemame or password.)
1. Click "My Account" in the myUFL systems menu.
2. Select "Update My Directory Profile." Once you are
on the Gatordex Self Service page, you may modify
the appropriate address information by clicking on the
orange hyperlinked text that reads "Addresses and Phone
Numbers."
3. To modify an address, select the radio button for
Permanent Home Mailing Address and/or UF Business
Mailing Address and hit submit. Make the necessary
change, and hit submit again. **IMPORTANT NOTE:
Foreign nationals are required to maintain a foreign
address for tax purposes and therefore should not modify
the Permanent Home Mailing Address which is reserved
for the foreign address. Instead, the Local Home Mailing
Address field should be usedfor the local address. Questions
regarding foreign addresses or tax implications should be
directed to the Tax Services Office at 392-1324.
4. If Permanent Home Mailing Address or UF Business
Mailing Address is not available, you must add the
address by clicking on "Add New Address and Related
Phone Numbers" found in the menu options located at
the top left of the page.
5. Fill in your updated address information and click
"submit" at the bottom of the page to complete the
address change.
Contact your directory administrator or the UF Help Desk
at 392-HELP or helpdesk@ufl.edu with questions pertaining
to the UF Directory. Need additional help? Visit http://www.
bridges.ufl.edu/training/docs/AddANewAddress.pdf for an
instruction guide.

Open Enrollment and Benefits Fair
Slated for Fall

This year's annual Open Enrollment and Benefits Fair is
just around the comer. Open enrollment is scheduled to
begin on September 19 at 8:30 a.m. and end on October 18 at
5:30 p.m. A Benefits Fair, which provides more information
about available programs and options, will be held on


September 25 and 26 in the Reitz Union Grand Ballroom.
Additional Benefit Fairs may be available closer to this area,
but a schedule is not currently available. As additional
information is received, the HR Office will try to make it
available to all employees.
Watch for Employee "State Sponsored" Benefits
Statements which will be mailed to home addresses
beginning September 5. The People First Service Center
will be available to respond to general inquiries about Open
Enrollment starting September 12. The Service Center staff
will also be able to provide information and assistance
in preparation for Open Enrollment, such as helping to
establish and reset passwords, etc., which are necessary to
log into the People First web site. However, they will not be
available to accept Open Enrollment elections or changes
until September 19.
Additional information will be provided inthe September
InfoGator. Questions? Please contact University Benefits
and Retirement at 392-1225 (SC 622-1225) or at benefits@
ufl.edu.

Packinghouse Day:
Decontamination Training
By Holly Chamberlain

Decontamination training session was held for
packinghousess at the Citrus REC, Lake Alfred on
August 17, 2006. The program was designed to review
decontamination procedures and regulations for the
2006-2007 fresh fruit harvest. Holly Chamberlain, UF/
IFAS CREC provided information and a review on
decontamination followed by Dr. Tim Schubert, FDACS-
DPI who led the discussion on regulations. Participants,
28 in total, will receive an updated certificate in the mail for
decontamination
training. It .
was decided by
FDACS-DPI that
this training is
now only required
every other year.
As presented in
the Citrus Health
Response Plan
(CHRP), citrus
packers must
register by signing Bins are decontaminated with an approved
the newly revised product and then remaining debris swept out
I with a broom.
compliance
agreements similar to last season that contain the
standard decontamination requirements. Trailers that do
not come into contact with citrus trees do not need to be
decontaminated.
Citrus-Producing States As of this writing, fresh
fruit movement to citrus-producing states will likely be
prohibited. The Department is still seeking a response to
our request to open some of these markets.


(cont. on page 4 Decontamination Training)







(cont. from page 1 Packinghouse Day)
regulations effecting the Florida citrus industry.
During his presentat.n i .nt l.l 'P,-,.:i,n nj Silpp1i-, F, .Lii
Florida Citrus in 2006-2: 11- D, TiS s.:I .t ,-,, t... FD[ CS
stated "This season will l.. [ ,- i t'..i- t n ...nl p.i ..i I'. :,' -
of the reluctance in other i .:i u- i p i..:, L.t-r JL. I [. ::. pt Fl. I ,I '.-
fresh fruit based on per : L.U. .1J :,iInl1 .I nit k ,ll, ,,i. 111111 ni
willbe considered a canli., I L.,i.n 'S:'.hultI .I nt- nu.L J 1 -'. ,i.
"Let's not give them awi. ,-iln11 it l 1n ,1 .:nt I th Ip., ,II I j
Like all other aspects
of this program, we -."ar A:
must work together r .'.
to accomplish this.
One failure could be a
setback for everyone."
Schubert stressed to
everyone that this
upcoming season will
represent the first
step in the process of
regaining unrestricted Approximately 150 attendees are welcomed.
market access for
Florida Fresh Fruit.
Dr. Tim Gottwald, USDA/ARS, continued during his
presentation, "Citrus Canker on Fresh Fruit," along the same
line as Schubert. "California, Texas, Arizona, Louisiana, Hawaii,
Puerto Rico, and the EU have all expressed concern and/or
are legislating to keep Florida fresh fruit, nursery plants, and
ornamental citrus relatives out of the marketplace in some of
those locations," stated Gottwald. The presentation showed
research for identifying lesions on freshly picked fruit and some
decontamination procedures prior to the packinghouse line.
Dr. Jan Narciso, USDA/ARS, in her presentation, "Reduction of
Xanthomonas on Surfaces," also followed similar research lines.
"New Fresh Citrus Cultivars for Florida," presented by
Dr. Jude Grosser, revealed a new mandarin hybrid that will be
available to the Florida growers. LB8-9 mandarin hybrid is a
mid-season maturing hybrid that combines medium fruit size,
attractive orange color, and good fruit flavor. Grosser explained
one economic advantage of the new hybrid would be the harvest
time of at least a month earlier than other similar varieties.
Other presentations were "Postharvest Peel Pitting Relating to
Rapid Changes in Relative Humidity," presented by Dr. Ferando
Alverez for Dr. Jackie Burns, "Preharvest Fungicides to Reduce
Postharvest Decay of Fresh Citrus," presented by Mr. James
Salvatore, and "New Maximum Residue Limits (MRLs) for Fresh
Citrus Export Shipments," presented by Dr. Mark Ritenour.
For copies of many of the presentations, visit the University
of Florida Postharvest Programs & Information website http://
postharvest.ifas.ufl.edu (click on previous events).
Before the afternoon events, attendees were treated to lunch
from The Happy Cooker, PMS of Greater Tampa, Inc. located in
Thonotosassa, which was sponsored by Cerexagri, Inc./DECCO
of Ft. Pierce.
In addition to the commercial exhibits in the Center's
packinghouse, a training session on packinghouse canker
decontamination was presented by Ms. Holly Chamberlain and
Dr. Tim Schubert at Ben Hill Griffin Hall.


(cont. from page 1 Etxeberria Lab Highlight)

other reproductive organs.
Central to all studies is the
determination of the mechanism of
sucrose (sugar) uptake into developing
fruits (and other developing storage
organs). "The necessary transport of
sucrose across several membrane barriers
upon its arrival to the citrus juice cells
has been investigated using purified
membrane systems and, most recently,
with the use of membrane impermeable
fluorescent markers, quantum dots and
I, nano-spheres," explains Etxeberria.
The newly acquired confocal
microscope at CREC has given this investigation a big boost
by allowing the visualization and qualitative measurements
of previously theorized transport processes. "For example,"
continues Etxeberria, "in Fig. 1,
forming endocytic vesicles can
be seen in a 3-D tomographic
analysis of a Citrus juice cell
during sugar uptake. Fig. 2 shows
a cytoplast demonstrating the
synchronous uptake of fluorescent
sugars across membrane-bound
carriers (green cytoplasm) and
fluid phase endocytosis (red
dots) using Nomarsky fluorescent Fig. 2
microscopy."
Sucrose uptake studies are paralleled by determinations
of the factors affecting citric acid accumulation and decline
in Citrus fruits. A multi-year research project is under
way to determine the changes in membrane bound citrate
carriers and channels, and the accompanying enzyme
activities during development of various Citrus fruits
differing in their capacity to accumulate and utilize citric
acid. Although found at much lower concentrations than
sugars, citric acid is a vital component of fruit quality.
Another study, presently being carried out in the Etxeberria
lab, focuses on the effect of mild water stress on Brix content
in Citrus fruit. "In field studies, we have demonstrated that
mild water stress enhances the accumulation of soluble solids
in citrus fruits without affecting yield," said Etxeberria. The
study also demonstrates the lack of effect on citric acid content
and a 2-week delay in flowering. Studies on the postharvest
shelf-life of fruit from mildly water-stressed trees is currently
being planned.
Sugar uptake studies in the Etxeberria lab are carried
out mostly by Pedro Gonzalez (Senior Biological Scientist)
and Diego Pozueta (visiting student). Diann Achor is a
close collaborator in aspects related to confocal and electron
microscopy, and Dr. Larry Parsons (Professor of Horticulture)
and James Holten (OPS) complete the team of collaborators
dealing with water deficit irrigation. Other close collaborators
of Etxeberria include Drs. Javier Pozueta and Edurne Baroja
(Pamplona, Spain), and Drs. Edna Negron and Felix Ramos
(UPR-Mayaguez).







Dr. Madhugiri Nageswara Rao
Recognized by
Genome India International

T he research work
Tof Dr. Madhugiri
Nageswara Rao in the field
of agricultural biotechnology
and forest genomics has
been recently recognized
as 'member-spotlight' by
iGenome India International
(GII; www.thegenomes.org),
an international organization
comprised of several reputed
Indian scientists and research
scholars (700+ members) from all over the world.
Dr. Rao's scientific research in India has contributed
significantly to the understanding of impacts of human
disturbances on the genetics of various forest species, mapping
for germplasm collection in medicinal and economically
important tree species, the role of protected areas and wild-life
santuaries, and identification of bioactive chemical compounds
in forest plants. The outcome of his studies, prehaps the first
of their kind in the Western Ghats (one of the mega diversity
'hot-spots' in the world), has important implications for future
conservation and sustainable management of various forest
genetic resources in India.
At CREC, Dr. Rao works with Dr. Fred G. Gmitter, Jr. as a
Postdoctoral Research Associate and he is currently working
on genetic improvement of Citrus for obtaining tolerance to
various diseases and environmental stresses using advanced
biotechnological tools.

UF/IFAS Faculty and County Extension
Agents Travel to Brazil

In July, a group of 10 county faculty and researchers
traveled to Brazil to gain a better understanding of
citrus canker and greening control practices. Citrus canker
has been endemic in the state of Parana since the 1950s, and
greening was more recently found in the state of Sao Paulo
in 2004. With both of these major citrus diseases present in
Brazil, practical lessons on management can be obtained by
visiting with and observing grower disease management
practices.
The week-long tour was planned and developed by
Drs. Steve Futch, Ron Brlansky, and Ms. Julia Beretta of
the CREC. Other tour participants were Drs. Kuang-Ren
Chung and Michael Rogers of the CREC; Gary England,
Chris Oswalt, and Dr. Mongi Zekri representing the county
agents; and Drs. Robert E. Rouse and Phillip A. Stansly from
the Southwest Florida Research and Education Center in
Immokalee. The tour began in the state of Parana with grove
visits arranged by Dr. Rui Leite, Plant Pathologist, from the
Institute Agronomico do Parana, Brazil.
These visits included seeing citrus canker in groves and


the production practices that have been established to
maintain adequate and profitable citrus production. For the
management of canker, growers in Parana have developed
extensive production practices as well as variety selections
to remain profitable while living with citrus canker.
Later in the week, the group visited groves in the
state of Sao Paulo to view greening and its management
practices. Growers in Sao Paulo have instituted extensive
grove surveys to identify and remove infected trees as well
as vector management and clean nursery trees to remain
productive and economically viable.
Drs. Brlansky, Rogers, and Stansly then attended the
Huanglongbing/Greening International Workshop in
Ribeirao Preto.

P~ri;


from left to right: Michael Kogers, Bob Kouse, Mongi Zekri, mhil
Stansley, Kuang-Ren Chung, Julia Beretta, and Ron Brlansky. Back row,
left to right: Steve Futch, Chris Oswalt, and hidden behind Ron is Gary
England. Others pictured are employees of Gaucho Farm.
(cont. on page 4 Decontamination Training)
Non-Citrus Producing States Fruit must originate
from a grove block that is inspected and found free of canker
within 30 days of harvest. The Department and the USDA
are taking pro-active measures to conduct inspections on a
timelybasis. Fruit
must be shipped
under Limited
Permit.
Fresh Fruit
Exported to other
Countries Fruit
destined to other
countries must
meet the receiving
country's entry
re quirements. Grapefruit being washed with SOPP for fresh
Europewillaccept fruit packing.
citrus from Florida if an inspection of the grove block and
immediate vicinity are free of citrus canker. Asian countries
do not classify citrus canker as a quarantine pest, but the
fruit must be free of citrus canker to meet basic phytosanitary
requirements. Contact the Department for specific details.
Fruit for Processing or Sale Within Florida There are
no restrictions. (Photos Courtesy: H.L. Chamberlain, UF/IFAS CREC)









CREC EMPLOYEE NEWS


Pens, Pencils, and Calculators
for South African Schools

Winter Haven High School (WHHS) students collected pens,
pencils, and calculators to be delivered to Berseba Primary
School, a needy school located north of Johannesburg in South
Africa. The project was organized by David Futch, a student
at WHHS, and Deborah Futch, a history teacher, in May 2006. i
The project provided in excess of 2,500 pens, 3,000 pencils,
and 275 solar powered calculators, all which were donated by
WHHS students to aid the South African needy primary school.
The donated items were delivered to the school by the Futch
family while on vacation in South Africa. This was the second
year of providing basic school supplies to needy schools, with The Futch family, pictured on the
last year's project donating items to a school in Peru. presented the much needed school
School.


Gretchen Baut is
CREC's newest
grandma (pictured
here with her
granddaughter). The
Baut/Holiday families
were joined this month
by the newest family
member, Chelsea
Holiday. Chelsea,
daughter of John and
Kasandra Holiday,
was born on August 7,
2006 and weighed 9 lb
10 oz. Congratulations
to all!


Send in \oour travels photos, fun photos, family news,
personal and school accomplishments, graduations,
school awards: Citrus Leaves Employee News section
is the place to share with others places you'vee been,
actionsn, conmmnunit service efforts, etc.


W


I supplies to the Berseba Primary


r. Kuo-Tan Li recently left Dr. Syvertsen's
program to take a faculty position in Fruit
Science in the Department of Horticulture at the
National Taiwan University in Taipei, Taiwan. Kuo-Tan
had a very strong background in whole-tree physiology
of apples from his Ph.D. work at Cornell and became
a postdoc here 2003. Kuo-Tan soon became the group
leader in the project dealing with effects of mechanical
harvesting and abscission chemicals on citrus tree health.
While here, he independently developed methods
to assess light absorption in citrus tree canopies and
subterranean methods to monitor root growth in situ.
His hard work
will be missed
L and he will
be difficult
to replace.
We will be
hearing more
From Kuo-Tan,
however, as
he owes Drs.
Syvertsen and
Burns several
Left to right: Dr. Jim Syvertsen, Dr. Kuo-Tan Li, manuscripts.
and Jill Dunlop (photo by Kathy Snyder)


WELCOME FOR CHANGE OF ADDRESS
Marcelo Neres, Student, Muraro Lawrence Bohannon, Volunteer, PLEASE NOTIFY:
Armstrong UF/IFAS/CREC
FAREWELL Lillian Brickman, Lab Tech, Grosser Attention: Kathy Snyder
Joshua Adkins, OPS, Rogers Jacob Butler, OPS, Parsons 700 Experiment Station Road
Ryan Atwood, Scientific Research Kelly Cook, OPS, Syvertsen Lake Alfred, FL 33850
Mgr., Gmitter Melinda Grosser, OPS, Graham
Diamond Basnaw II, Volunteer, Kuo-Tan Li, Postdoc, Syvertsen OR E-MAIL:
Hoover Levette Rucks, OPS, Graham SNYDERKM@UFL.EDU
Xiao Zhang, OPS, Timmer























































Sept. 4 HOLIDAY LABOR DAY
Sept. 6 Seminar: Dr. Michelle Danyluk,
Candidate Food Microbiologist, Rm 2, BHG,
11:00 a.m. 12:00 noon.
Sept. 11 Hi-Tech, Mike Evans, Rm 2-4,
BHG. 10:00 a.m. 1:00 p.m.
Sept. 12- Rural Water, Don Hamm, Rm 3-4,
BHG, 8:00 a.m. 4:00 p.m..
Sept. 18 Faculty Meeting, Dr. Harold
Browning, Rm 3-4, BHG. 8:30 a.m. to 10:30
a.m. (TENTATIVE)
Sept. 19- Seminar: Dr. Lukasz Stelinski,
Entomologist Candidate, Rm 2, BHG, 11:00
a.m. 12:00 noon.
Sept. 25 Seminar: Dr. Juan Alvarez,
Entomologist Candidate, Rm 2, BHG, 11:00
a.m. 12:00 noon.


Sept. 27 Seminar: Dr. James Harwood,
Entomologist Candidate, Rm 2, BHG, 11:00
a.m. 12:00 noon.


Updates and Changes can be
seen on the web at
httap: \'ww .c.rec. Lillzti .edli Iiew'

Citrus Leaves Submission Deadline
September 21, 2006

Training Offered


No schedule at this time




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