Title: Citrus leaves
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Title: Citrus leaves
Series Title: Citrus leaves
Physical Description: Serial
Creator: Citrus Research and Education Center, Institute of Food and Agricultural Sciences
Publisher: Citrus Research and Education Center
Publication Date: August 2004
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Bibliographic ID: UF00087049
Volume ID: VID00044
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.

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Sir DIVERSITY OF
F LORIDA

IFAS



Citrus Leaves.


= WW .RC1AS.F. 3


Dr. Harold W. Browning, Center Director
UF/IFAS Citrus Research & Education Center
700 Experiment Station Road
Lake Alfred, FL 33850-2299
Tel. (863) 956-1151
Fax (863) 956-4631


August 2004


UF/FA Ciru Reeac an Edcto'etr-Nw Inomtn Voum 22 No


In This Issue
Meet the Grad Student Aug. 11 ........ 1
Citrus Pathology Fall 2004 ................ 1
Packinghouse Newsletter 200 ......... 1
43rd Annual Citrus Packinghouse
Day ....................................... 1
Meet ... Dr. Chung's Lab ................. 2

Meet CREC's Students ................. 3-5
Congressional Staff Visit ................. 6
Travels ....................................... 6
Kanjana at USDA ............................ 6
News Around CREC ....................... 7
Welcome, Farewell; Rudene Scott
Retires; Team B Brings In Over $2500
C alendar ........................................ 8





school and college
students at CREC!
Pages 3-5



Manuscripts Submitted to the
Publications Committee in July will
be published in the September
issue.



Citrus Leaves
is the monthly newsletter for
employees and friends of CREC.
Citrus Leaves welcomes your
contributions, suggestions and
corrections. Editor, Monica
Lewandowski; E-mail
mmlew@crec.ifas.ufl.edu; Ext.
1233. Writer, Meredith Jean
Morton. Photography and
graphics, Gretchen Baut;
Production and Distribution: Word
Processing, Barbara Thompson,
Supervisor; Kathy Snyder, Karla
Flynn and Linda Murphy;
Customer Service, Kathy
Witherington, Supervisor, and
Nancy Burke.


You are invited -
IstAnnual
Meet the CREC Graduate Students
Symposium

Wed., Aug. 11
10 am 12 noon

CREC BHG Rooms 1-2

* All personnel invited; refreshments will
be served

* Research poster symposium for CREC
graduate students and students in
affiliated programs about a dozen
participants and their faculty advisors
will be on hand

* Excellent opportunity to meet our grad
students in food science, plant
pathology, horticultural sciences,
agricultural and biological engineering.
Hope to see you there!


Citrus Pathology at CREC
PLP 5115C Section. no. 2172
Tuesday 3-6 p.m., Aug. 24-Dec. 14
BHG Teaching Lab
Topics include viruses and viroids, fungal
pathogens, prokaryotic pathogens, citrus
canker, disease control, exotic diseases, root
health, postharvest diseases and disease
resistance in plants. The course is coordinated
by Dr. Ron Brlansky and will be team taught by
Drs. Brlansky, K.R. Chung, Bill Dawson, Ken
Derrick, Jim Graham, Pete Timmer, Mark
Ritenour, John Zhang, Jude Grosser, Dennis
Lewandowski and Siddarame Gowda. For the
course schedule, please visit:
www.crec.ifas.ufl.edu/Brlanskv/PLP5115C.htm
Prerequesite: a basic introductory plant
pathology course or permission of the
instructor (contact Dr. Brlansky). Fees for 3.0
unit graduate course (Fla. resident) is $685.44
based on $228.48 per credit hour (subject to
correction).
Registration is August 19-20; the first class is
Aug. 24. For registration information, visit
disted ifan nlfl edCi


____________________________________________________________________________________ I _______________________________________________________________________________


Packinghouse Newsletter
Celebrates 200
www.postharvest.ifas.ufl.edu
The Packinghouse Newsletter, currently
edited by Dr. Mark Ritenour (Indian River
Research and Education Center), provides
information to the fresh citrus industry. July
2004 marked the 200th issue of the newsletter
that was initiated by Dr. Bill Grierson, retired UF/
CREC professor, in 1965. In this milestone issue,
Dr. Grierson recalls one fateful day in 1965 at a
citrus packers pre-season meeting. After
answering "a slew of questions," someone
suggested that Dr. Grierson send out his "good
information" regularly. He got together with Dr.
Andy McCornack at CREC and drew up the first
Packinghouse Newsletter. Over 100 copies were
produced on a mimeograph machine. As the
mailing list grew, he requested that recipients
send self-addressed, stamped envelopes, but
later had to convert to a University mailing
see Packinghouse Newsletter p. 7


Sept. 2 43rd Annual
Citrus Packinghouse Day
Seminars, workshops and vendor exhibits for
the fresh citrus industry. Topics: how to
pass 3rd party food safety audits, brief info
on Eurepgap and British Retail Consortium
(BRC) requirements (Keynote speaker Juan
Muniz with Primus Labs); packinghouse
biosecurity; prospects for good fruit quality
this year; use of color separation before
degreening; prospects and progress on
robotic harvesting for fresh citrus; radio
frequency identification (RFID) tagging;
prevention of physiological disorders.
Supplemental training sessions on:
Food safety, worker health and hygiene
Forklift driving safety
Packinghouse postharvest treatments:
biocides, waxes, recordkeeping, hygiene and
environmental safety.
Free, pre-registration requested:
Dostharvest.ifas.uf.edu.








-2

Meet Dr. Chung's Lab by Meredith Jean Morton Photos by Gretchen Baut
Dr. Kuang-Ren Chung and his laboratory team conduct research in the area of plant pathology specifically, they are working to
understand the molecular mechanisms involved in fungal pathogenicity, or the ability of a pathogen to cause disease. Their work primarily
involves molecular methods to identify fungal genes that are involved in infection of citrus.
Some of the lab's current projects are aimed at the involvement of plant hormones in the citrus postbloom fruit drop (PFD) caused by the
fungus, Colletotrichum acutatum, and the potential for using hormone inhibitors for disease control. Dr. Chung said a goal of his research
is to "establish the involvement of hormones such as ethylene, abscisic acid orjasmonic acid in the occurrence of PFD."
Chung said the long-term goal of this project is to prevent fruit drop and ultimately reduce losses. Another aspect of research in Dr. Chung's
lab is to identify novel compounds that can be used for disease control in citrus. With 30 percent extension work to accompany his research,
Chung wants to provide feedback and results to growers, helping them to learn about PFD and other fungal diseases.
Dr. Chung's lab, located on the first floor of the administration building, is very international. Dr. Chung is from Taiwan, and he has a post
doc from France, as well as a visitor scholar from China and lab technicians from China and Florida.


Dr. Chung


Vivek Gowda and Kate Lahey Dr. Mathias Choquer Dr. Lihua Cao and Huiqin Chen


Dr. Kuang-Ren Chung has been a
faculty member at CREC since 2000. As a
native of Taiwan, Chung came to the United
States in 1991 to pursue his Ph.D. in plant
pathology at the University of Kentucky.
Chung subsequently went to North
Carolina State University for postdoctoral
work on fungal molecular genetics on fungal
toxins produced by Cercosporin.
Raised on his parents' rice farm in
Taiwan, Chung said he realized he wanted
to do something with agriculture; however,
working in the rice fields as a child, he
discovered he did not want to work outside.
Chung said while majoring in plant
pathology he learned how much he truly
enjoyed the "indoor aspect of agriculture."
Chung is very devoted to his family and
takes pride in them. Chung and his wife
Lisa came to the United States after they
had been married for six months. He said
he was especially grateful for her at that
time because she was the only person in
the United States that he knew! Chung
enjoys spending time with his children, 10-
year-old Tony and 6-year-old Casey.

Dr. Mathias Choquer is a
postdoctoral scientist who has been
working in Dr. Chung's lab for 3 months.
From Paris, France, Choquer hopes to stay
in the U.S. and at CREC for the next 2-3
years to conduct research in fungal
molecular biology.
CrrPntlyv Chonner is working on the


cloning and characterization of fungal
pathogenicity genes from Colletotrichum
acutatum causing key lime anthracnose, a
disease that is closely related to PFD. While
PFD primarily affects citrus flowers, Key lime
anthracnose affects both leaves and flowers
on Key lime, raising some intriguing
questions about the processes involved in
pathogenicity.
Choquer earned his Ph.D. in plant
pathology from the University of PARIS in
March, 2004. His research involved chitin
metabolism versus fungal virulence in the
phytopathogenic fungus, Botrytis cinerea.
When in France, Choquer enjoyed traveling
in the countryside because he enjoys the
landscapes and French architecture. Since
he's been in theUnited States, he has toured
Florida a little, but would like to see more of
the country. Choquer also likes movies.

Dr. LiHua Cao is a visiting scholar
from China who has been in Dr. Chung's lab
for about a month. Cao is currently working
on the mechanisms of pathogenicity in
Colletotrichum acutatum causing
postbloom fruit drop.
Cao earned her Ph.D. in plant pathology
from the Northwest Sci-Tech University of
Agricultural and Forestry, China in 2003.
This is Cao's first time in America. She
has a scholarship from the Chinese
government to study here for two years.
Cao enjoys listening to Chinese modern
music swimmin and reading books


Kate Lahey has been working inDr.
Chung's lab since graduating from Florida
Southern College in 2003 with a bachelor's
degree in landscape design.
Originally from Pittsfield, Mass., Lahey
also lived in Naples, Fla. for a few years
before attending Florida Southern.
In Dr. Chung's lab, Lahey has been
working on projects with molecular biology,
gene expression of citrus in response to
fungal infection, and the involvement of
hormones inpostbloom fruit drop. She will
soon begin a project to find signal
transduction genes from Mycosphaerella
citri, the cause of greasy spot, involved in
fungal pathogenicity. Once a genes) is
cloned, functional characterization of the
gene will begin.

Huiqin ChenjoinedDr. Chung's lab
in November 2003 to conduct a research
on the functional determination of the gene
related to Colletotrichum pathogenesis.
Chen earned her master's degree in plant
physiology and Biochemistry from the
Central China Agricultural University.
Chen and her husband, Chunxian, who
works in Dr. Gmitter's lab, are the proud
parents of Manzhen, 7, Mandi, 4, and
Manning, 2.
In her free time, Chen enjoys playing
and reading with her children.

see Dr (C m,,i's Lab, p. 5


Curr Iv honur isworkray n th musc swmmin- an reaini-book







Student Life ... by Meredith Jean Morton/Photos by Gretchen Baut
Among our CREC employees are many bright and talented high school and
college students. They have a wide range of interests and aspirations, but are all
highly motivated towards their educational and career goals. They work at CREC
and perform a variety of jobs ... for the experience, as well as the paycheck. They
are the scientists, doctors, engineers, musicians, artists and business execs of our
future. Read on the learn more about ... students at CREC.


Candace Abou-Tabl has been .
working inDr. Dawson's lab since May
2002. She said she "does a little of
everything," including maxi preps,
tissue collection, purification of L. t
proteins, western blots, running gels, I
and helping in the greenhouse. .. -
Candace just graduated from Polk -
Community College with her AA in :
biological sciences, and hopes to attend .
the University of Florida in fall 2005. .
When she isn't busy working, Candace said she loves to spend
time with her two nieces and nephew, go to the movies with her
husband Emad, read, and learn to cross stitch and crochet. She also
loves to cook and likes to try cooking different ethnic foods. Candace
said she loves herjob at CREC and would like to come back after
graduating from UF to work in research.
Josh Adkins is a junior at Florida Southern College majoring in
horticultural sciences. A 2002 graduate of
Bartow High School, Josh has been working
for Dr. Buker the past two summers in weed
research, as well as mechanical harvesting
studies. After earning his bachelor's degree
from Florida Southern, Josh plans to attend
the University of Florida for graduate school.
Josh enjoys hunting and fishing.


Sylvia Arnold has been working in Dr.
McCoy's lab since May during her summer
break from Auburndale High School. The
Aubumdale senior has been doing field work
with Diaprepes and ants, including trapping
and analysis. Sylvia, who enjoys swimming
and reading English literature, is interested
in pursuing a bachelor's degree at the
University of Florida in either political science
or nuclear physics. After completing four
years at UF, Sylvia said Icshe would Iikc Io
attend graduate school i1 Fl.lic'c .I inollOci
European country. Syl\ lu'. ixill nl .ii Di
Arnold and Rhonda Sc lullldiii


A bass player in his
band, Luxembourg,
George enjoys
music, as well as art,
reading and writing.
Jeff Browning
has been working at
CREC forthree years


Are you a student, or do you know
a student at CREC? Contact
Monica Lewandowski tel. ext. 1233
or (mmlew@crec.ifas.ufl.edu or) and
we'll include you in a future issue.
And ... hope you can attend
CREC's "Meet the Graduate
Students" Symposium on Wed., Aug.
11, from 10 a.m.- 12 p.m. inBHG
Rooms 1-2. About a dozen graduate
students from UF and affiliated
programs will be on hand.
Refreshments will be provided!


during his breaks from the University of
Florida, where he is studying biology. In
Dr. Grosser's lab, Jeff said he has been
working on a genetic analysis project,
along with other general lab work, and a
little bit of field work. Jeff is majoring in
biology, but said his "application for it in
future is yet to be decided." Jeff enjoys
art and playing and listening to music; he
is the son of Center Director Dr. Harold
and Nancy Browning.


p


George Brinkley is a 2004 graduate of -
Haines City High School and began working a
in an OPS position in the Photolab in mid-
June. In the Photolab, George has been
scanning photographs and slides, as well
as doing photo editing. George plans to
attend Polk Community College in the fall
and major in graphics. He has experience in
computer photo-editing from classes at PCC
and working on his high school yearbook.


cf l
S


I


Baylis Carnes is a 2003 graduate of
Winter Haven High School and has been
working for Dr. Syvertsen for a year and a
half. Since Baylis began working in the lab,
he fertilizes plants, helps the postdoctoral
scientists, collects data and organizes the
lab. Baylis is attending Polk Community
College, pursuing a degree in Business
Administration. An avid outdoorsman, he
enjoys fishing and hunting. Baylis said he
likes working inDr. Syvertsen's lab because
he has been exposed to a variety of cultures
from the lab's international diversity.
Patrick Dees has been working for Dr.
Albrigo since February spraying fertilizer,
collecting well samples, counting fruit
harvest, and working in the greenhouse. He
also does a fair amount of driving in the
"massive F150" truck. The 2000 Winter
Haven High School graduate is studying
online to be a web designer through
Education Direct. In his free time, Patrick
builds historic models that he sells on e-
Bay.
Claire Denlinger is a sophomore at
Emory University in Atlanta, and has yet to
choose a major since Emory encourages its
students to take two years to explore their
options. The 2003 Winter Haven High
see More students, p. 4


Ov7
;.


I







More students... from p. 3
School graduate has been working at CREC for Dr. Albrigo since
June doing data collection and recording. Claire (left) just returned
from a trip to Europe, where she toured France and had the
opportunity to see many of its famed sites.

April Dozier has been working in Dr. Albrigo's lab since June
doing data collection and recording.
April is a 2003 graduate of Florida State
University, with a bachelor's degree in
biochemistry. The Lakeland native is
now taking courses at Hillsborough
Community College, hoping to earn a
license in nuclear medical technology.
April enjoys reading and visiting her
sister and nieces
and nephews.

Traliva McGinty (center photo, seated
left) is a sophomore majoring in biology at
Stetson University. The 2003 Haines City High --
School graduate worked inDr. Nigg's lab this
summer running experiments on Diaprepes
root weevils and fruit flies. Traliva would like
to be an ob/gyn or a pharmacist.

Savannah Foltz (photo above right, seated
right) graduated from Harrison Arts Center in
2004. With plans to attend Florida Southern College in the fall,
Savannah has been working in Dr. Nigg's lab since June. In Dr.
Nigg's lab, Savannah records experiments on the Diaprepes root
weevil and fruit flies. She also extracts flies and counts eggs.
Savannah is a vocalist and will be majoring in music education at P
Florida Southern and wants to be an elementary music teacher. i

James Holeton has been working in Dr. Parsons' lab since
November 2003, generally assisting in the lab with soil analysis
and data collection. The 2001
Graduate of Winter Haven High
School received his AA degree from Polk
SCommunity College inMay 2003, and will
Soon begin classes at University of
South Florida to complete his degree in
mechanical engineering. After earning
his degree, James said his goal is to 'get
Ba job using the skills he's learned.' In
what James said is practically nonexistent
free time, he enjoys building mechanical
models.
Lily Kender is a sophomore at the
University of Florida, majoring in accounting,
with hopes of owning her own accounting
firm. Lily worked at CREC in Dr. Singh's lab
during May and June. During her time at
CREC, Lily helped to update and design Dr.
Singh's website and PowerPoint
presentations. When she isn't studying or
busy with schoolwork, Lily said she loves
tennis and dancing. Lily is the daughter of
retired CREC center director, Dr. Walt Kender,
and mother Carole.


Meredith Jean Morton is a junior majoring in magazine
journalism at the University of Florida. She is working with Dr.
Monica Lewandowski as a public relations summer intern, and has
,. enjoyed writing magazine and newsletter
articles on CREC research and other citrus-
related topics. The 2002 Winter Haven High
School graduate is at CREC for the second
S consecutive summer, after working in Dr.
SRouseff's lab last year. Meredith participated
in school science fairs injunior high and high
school (with guidance from various CREC
faculty, including Dr. Nigg), winning honors
at the school, county and state level. She
competed at the State Science and
Engineering Fair of Florida for six straight
years, and was selected to take her project to
the international science fair for two years.
SY.-. ; Meredith aspires to be a science writer for a
-. popular magazine or other mainstream
publication.

She Steve Nagy (lower left) is a senior at
Auburndale High School, working in Dr.
Nigg's lab. Steve has been working for about
\ a year "extracting flies", counting dust mites
jin the grove, and doing a variety of other
laboratory tasks. Forward onAuburndale's
varsity soccer team, Steve said the team is
"pretty good this year.'" Steve plans to attend
the University of Central Florida after
graduation to study either electrical or
, computer engineering. His father, Steve Nagy,
was a chemist with the FDOC at CREC.

rl Jessica Noling is a 2004 graduate of
fl Aubumdale High School and will attend the
S University of Florida in the fall. This is the
second consecutive
summer Jessica has
workedat CREC for Dr. Graham. Jessica
said she helps with soil samples, runs PCRs,
extracts RNA, runs electrophoresis gels,
and 'lots of exciting stuff,' in addition to
washing dishes and working in the
greenhouse. At UF, Jessica plans to major
in forensic sciences or microbiology; she
wants to be a forensic scientist. Jessica
said she is a professional tuber, and just
"likes to have a good time.'" Jessica is the
daughter of Dr. Joe and Roxanne Noling.


Phillip O'Neill graduated from Poinciana
High School and started working in Dr.
McCoy's lab in August. In high school, he
was on the track and cross-country teams
and will enter the Air Force in October.

Amanda Parker has been working at
CREC inDr. Grosser's Lab since January of
see And more students, p.5







And more students... from p. 4
2003. Amanda has worked on ploidy analysis using FLOW
cytometry, and also works in the greenhouse
twice and week. Currently she is learning
protoplast transformation techniques.
Amanda graduated from Auburndale High S
School in May, and plans to attend the
University of Charleston in West Virginia in
August. She plans to major in biology and
then attend graduate school in veterinary ,-
medicine. In her free time, Amanda reads
books and enjoys concerts. --

Monica Puentes is a senior at
Auburndale High School and has been working at CREC for Dr.
Gmitter since May. In the lab, Monica extracts DNA and RNA as a
part of a project to determine the
susceptibility of plants to citrus tristeza
virus. Monica plans to attend either the
University of Tampa or the University of
Florida after graduation from high school,
building on the credits she already has from
her current dual enrollment at Polk
Community College. She would like to be a
pediatrician.
Nolan Rayburn graduated in May from
Winter Haven High School, and has been
working in Dr. Nigg's lab for about two years doing miscellaneous
lab and field work with Diaprepes, fruit flies and mites in the groves,
what Rayburn refers to as 'all the fun stuff.' He has been taking
classes at Polk Community College through the Dual Enrollment
program during high school, and will complete his Associate's
degree in animal science in December.
Following graduation from PCC, Rayburnm -
plans to attend the University of Florida to
major in a biology-based animal science.
Rayburn would like to be a veterinarian like
his father, but acknowledges the difficulty
vet school will bring, so he says he will
"wait and see what happens." In his free
time Rayburn enjoys hunting and fishing,
and was the 2003 Teen Anglers National
Freshwater Champion and 2004 runner-up.
This past spring, he won the 2004 Florida
Freshwater Regional Series points championship.

Carolina Sarmiento is a senior at the University of Florida
majoring in agriculture and biological engineering and will graduate
this summer. She has doing data collection and recording in Dr.
Albrigo's lab this summer. Carolina keeps
very busy, working at CREC during the week
and at Disney's Animal Kingdom on the
weekends. After graduating, Carolina plans
to travel for a semester, hoping to visit Peru
and other places in South America. In
January, Carolina will begin an internship
with Disney where she hopes to work on
their autoCAD program for the irrigation
system at The Land at EPCOT In the little
free time she has, Carolina enjoys traveling ,


and photography.

Rick Timpe has been working in Dr. Albrigo's lab for about nine
months spraying fertilizer, conducting fruit counts, collecting water
samples, and "keeping track of harvest." Ajunior at the University
of South Florida, Rick is studying business and social science. After
graduation, Rick plans to have a
government job for a while to gain
experience with plans to open his own
business later. The 2001 Lake Region
High School graduate has been playing
roller hockey since he was 10 years old,
and modestly said his Tampa team "is
pretty good."

Lee Tomlinson graduated from Winter
Haven High School in 2003 and has been
working for Dr. Miller since January 2003. In Dr. Miller's lab, Lee
works with Variable Rate Technology for fertilizer spreaders and
other miscellaneous jobs. Currently Lee is attending Polk
Community College, but plans to go to
Santa Fe Community College in
Gainesville in January for "a change of
scenery." After earning his AA from
Santa Fe, Lee will attend the University
of Florida for a civil engineering degree.
I When he isn't working or taking
., A classes, Lee enjoys golf and fishing,
and won top honors in the second
annual Polk County Teen Angler
Classic last year.


Dr. Chung's Lab ... from p. 2
Vivek Gowda has been working in Dr. Chung's lab for about
a month, "helping out wherever I can."
Currently he is helping Dr. Chung to develop
a gene disruption construct that will be useful
to determine the function of the gene.
Gowda is a senior in the International
Baccalaureate program at Bartow High
School who enjoys playing tennis and said I
what he does at CREC is "pretty fun."
Gowda would like to attend medical
school in the future, but is not certain where
he will pursue his bechelor's degree; he
plans to 'see what comes up' during his senior year. Gowda is the
son of Dr. Siddarame Gowda who works in Dr. Dawson's lab.


Welcome back to Dr.
Iqrar Khan (right, with
J.L. Chandler) from
Sultan Qaboos
University in Oman. Dr.
Khan is a visiting
scientist at CREC for 6
weeks, working with Dr.
Jude Grosser.


















CREC Hosts Congressional Staff Congressional staff members from the offices of eight Florida legislators (far right photo)
visited CREC on July 1 as part of a legislative tour of Florida's citrus industry. Dr. Mickey Parish (far left photo) presented an overview of
CREC programs, including federally funded programs for the Diaprepes root weevil, citrus canker and citrus tristeza, as well as food safety
and biosecurity. The meeting also included presentations by Dr. Dan Gunter, executive director of the Florida Department of Citrus, who
discussed market trends and strategies for Florida citrus products. Michael Yetter (FDOC) addressed citrus export issues, Robin Bryant
(FDOC) presented information on the citrus mechanical harvesting program, Dr. Jackie Burns (CREC, second photo from left) discussed
abscission research for mechanical harvesting, and Squire Smith (Florida Citrus Mutual, second photo from right) discussed international
trade and the importance of the tariff on imported citrus products for Florida citrus growers.


Traveling...
Dr. Russell Rouseff visited and lectured at
Uludag University during a recent visit to
Turkey. He also presented a talk at the Third
Charalambous International Flavor
Conference in Samos, Greece.
April Elston, UF Ph.D. candidate, presented
a poster, "Determination of aroma activity
of Valencene in orange oil peel," by Elston,
Dr. Jianming Lin and Dr. Russell Rouseff
the Institute of Food Technologists annual
meeting, July 12-16, in Las Vegas.
Dr. Elisabeth Knapp presented a poster,
"Role of elements located within the
methyltransferase domain in Tobacco
mosaic virus defective RNA accumulation,"
by Dr. Knapp, Dr. Gregory Danyluk, and
Dr. Dennis Lewandowski at the American
Society of Virology annual meeting in
Montreal, July 9-15.
Dr. Dennis Lewandowski presented a
poster, "The tubule forming NSm protein
from Tomato spotted wilt virus supports
long-distance movement of Tobacco
mosaic virus in tobacco in the presence of
the TMV coat protein," by Dr.
Lewandowski and Dr. ScottAdkins (USDA
ARS, Ft. Pierce), at the American Society of
Virology annual meeting in Montreal, July
9-15.
Dr. Vladimir Orbovic presented a poster,
"Genetic transformation of Citrusparadisi
(cvs. Duncan, Flame, Marsh, and Ruby Red)
with p23 sequence from the genome of
Citrus Tristeza Closterovirus" by Orbovic,
Dr. Ananthakrishnan Govindarajulu, and
Dr. Jude W. Grosser at the American
Society of Plant Biology annual meeting in
Orlando, July 25-28.


+ II i .i







Pedro Gonzales (above) presented a poster
at the International Workshop on Plant
Membrane Biology in Montpellier, France,
July 6-10. Pedro presented research done at
CREC with Dr. Ed Exteberria and with
colleagues in Pamplona, Spain (Pedro is
pictured with some of them at the meeting,
above right). The meeting included a
presentationby Prof. Roderick MacKinnon,
winner of the 2003 Nobel Prize in Chemistry
for his work on potassium channels. "The

Kanjana atUSDA ".-
by Meredith Jean Morton '
Dr. Kanjana Mahat-
tanatawee graduated from UF
with her Ph.D. in food science in
May and is now working at the
USDA Agricultural Research
Service (USDA-ARS) Citrus and
Subtropical Products Laboratory
in Winter Haven. She is
conducting researching on phytochemical
and flavor compounds in tropical fruits, such
as guava, mango, papaya, and carambola,
with Drs. Elizabeth Baldwin, Kevin Goodner,
John Manthey and Gary Luzio.
After coming to the U.S. from her native
Thailand in 1999, Mahattanatawee spent two
years at UF's Gainesville campus, followed
by three years at CREC under Dr. Rouseff's
instruction. Her research involved the


Conference was very
productive and let us to
meet many people
working in the same area
of plant membrane
biology," wrote Pedro.
After the conference, he
visited friends in Spain,
including Diego Pozueta,
who was a visiting
student at CREC a couple of years ago.
After the meeting, Pedro visited
friends and colleagues in Pamplona and
even partake in the city's San Fermin
festival, a two-week celebration in the
Spanish tradition, famous for the "Running
of the Bulls." (Pedro says he stayed a safe
distance away from the bulls.) He also
visited Barcelona, San Sebastian, Victoria,
and Bilbao, as well as Nimes, France, and
overall had a wonderful trip.

carotenoid
sa f breakdown products
S in citrus and effects
on juice flavor.
Carotenoids are the
yellow-orange
pigments found in
citrus and many
plants. While
carotenoids
generally are not considered to impact
flavor, Mahattanatawee and Rouseff's
research focused on breakdown
compounds from carotenoids
(norisoprenoids) that do impact orange
juice aroma and flavor.
Before coming to Florida,
Mahattanatawee was a faculty member in
the food technology department at Siam
see Kanjana, p. 7





NEWS A N


CREC Welcome
Esther Dunn UF Doctor of Plant Medicine
grad student (Dr. Timmer)
Dr. Ozan Gorbus visiting scientist (Dr.
Rouseff)
Marcela Frata visiting student (Dr. Rouseff)
Shinya Kanzaki visiting scientist (Dr. Gmitter)
Dr. Iqrar Khan visiting scientist (Dr. Grosser)
CREC Farewell
Juan Gabriel Perez (Dr. Syvertsen)
SylviaAmold (Dr. McCoy)
Dr. Jinhe Bai (FDOC, Dr. Dou)
Gary Coates (FDOC, Dr. Dou)
Merritt Daughtery (FDOC, T Long)
Virgil Stewart (FDOC, T Long)
Cynthia Holbrook (FDOC, Dr. Ismail)
Robert Gallagher (FDOC, Dr. Stinson)
Rosemary Hammond (FDOC, Dr. Cancalon)
Bill Lints (FDOC, Dr. Cancalon)
The FDOC laid off a reported 17 people in
Lakeland and Lake Alfred to meet a nearly $10
million budget reduction for 2004-05. The
research division had nine layoffs, three
vacant positions eliminated and two upcoming
retirements (Dr. Ismail and Dr. Nikdel).

DearCREC,
Thank you for the thoughtful
condolences I received following the death
of my father. One is never quite ready to
lose a parent. Your kindness and concern
have been appreciated.
Larry Parsons

Kanjana ... from p. 6
University in Bangkok, where she taught
classes in industrial microbiology and
fermentation technology.
It was during her five years teaching at
Siam University that Mahattanatawee re-
alized she wanted to pursue her Ph.D. in
food science. She said she taught a food
science student and discovered that to be
a more effective teacher she would need to
further her education on the subject.
The desire to learn and accept new chal-
lenges has been a motivating factor
throughout Mahattanatawee's educational
career. In high school she said she wanted
to study science because of its realistic ap-
plications and the challenges of discov-
ery.
Mahattanatawee accepts challenges in
other areas of her life as well, such as try-
ing new cooking recipes in her free time
and sightseeing while in the US.
With her new job at the USDA-ARS,
Mahattanatawee will be challenged as well.
Although she has extensive experience


wof*A _& I'7


We miss you, Rudi! Fond memories,
gifts, refreshments and lots of friends and
family were on hand to wish farewell to
Rudene Scott (above) on June 30, who
retired after 25 years at CREC. Rudene's
husband, children and grandchildren were
in attendance, lending a special touch to
the occasion. For more on Rudene, see her
profile in the June issue of Citrus Leaves.

with citrus, this is her first work with tropi-
cal fruits.
Mahattanatawee's interest in this
project is two-fold: first, her research
involves studies on the phytochemical
compounds considered beneficial to the
human diet; and second, tropical fruits are
important crops in both Florida and
Thailand. After completing a one-year at
the USDA laboratory, Mahattanatawee will
resume her position at Siam University in
Thailand, taking with her a wealth of
knowledge and experience.
After six years away, Mahattanatawee
said she will be ready to go home. "My
family is there, and everyone is waiting for
me to come home," she said with a smile.
"I'll be ready.

Packinghouse Newsletter ... p. 1
system. Dr. Grierson went on to produce
the first 23 Packinghouse Newsletters. He
retired from CREC in 1982, but is still attends
CREC events.
In 1969, Dr. Will Wardowski was hired at
CREC as the first postharvest Extension
specialist the first position of its kind in
the world. Shortly after arriving at CREC,
Dr. Grierson handed him the responsibility
of producing the Packinghouse Newsletter,
as well as organizing the annual Citrus
Packinghouse Day. Dr. Wardowski
produced many more (167 to be exact)
Packinghouse Day newsletters and
established a 31-year career serving the
fresh citrus industry. He retired from CREC
in 1999, and lives in Longboat Key with his
wife, Christie. He still maintains a book
business, Florida Science Source.
Dr. Mark Ritenour at the UF/IFAS Indian


Team "B" brings in over $2500
Many of you made donations in
memory of CREC s Beatriz Nielsen, who
died of cancer on May 24. Bea's brother,
Christian Nielsen Palacios, from Ithaca,
N.Y, participated in the American Cancer
Society's Tompkins County Relay for Life
on July 9. Relay for Life is a walk/jog and
a fundraiser for the American Cancer
Society, held in communities throughout
the U.S. Participants seek donations or
pledges, and join cancer survivors for a
walk orjog around a racetrack or trail during
the night-long event.
Christian was the top individual fund-
raiser in Tompkins County, bringing in
more than $2500. This was, by far, the
largest amount collected by an individual
in this event. The Nielsen-Palacios family
gratefully acknowledges the donations
made inBea's memory.


Our deepest sympathy to Wendy Bell on
the passing of her father in Jamaica.
Wendy is a graduate student in Dr.
Rouseff's lab.


I smIw l .
Citrus postharvest research and extension has a
rich history, including 200 issues of the
Packinghouse Newsletter and the annual Citrus
Packinghouse Day. Much of this work has been
conducted by (photo, left to right): Dr. Bill
Grierson, who retired from CREC in 1982; Dr.
Mark Ritenour at the Indian River REC in Ft.
Pierce, and Dr. Will Wardowski, who retired
from CREC in 1999.

River REC in Fort Pierce carries on as editor
of the Packinghouse Newsletter. It is now
available by e-mail and online, and the
current, 200th issue includes interesting
retrospects on the history of the newsletter
by Dr. Grierson, Dr. Wardowski and David
Hall. This and past issues are available online
at postharvest.ifas.ufl.edu.
Another long tradition continues, with the
43rd Annual Citrus Packinghouse Day on
Sept. 2 at CREC. The event is free and open
to the public; pre-registration is requested
and forms are online at
postharvest.ifas.ufl.edu.


I


I







Sun Mon Tues Wed Thurs Fri Sat


calendar.ifas.ufl.edu FloridaAgCalendar.com
UF/IFAS Extension / Ag industry events
events statewide u/ statewide



1 2 3 4 5 6 7






8 9 10 eetetht ral 1 12 13 14
10 am 12 pm Blood Drive at
Grad student CREC south
Crop Insurance recruitment parking lot
meetings
Crop Insurance

15 16 17 18 19 20 21
Citrus Canker DEP workshop
workshop -
decontam
training
materials

22 23 24 25 26 27 28
Faculty
UF classes Citrus Expo Citrus Expo mtg
begin Ft. Myers Ft. Myers
Citrus Pathologj
course 3-6 pm

29 30 31 Sept. 1 Sept.2
Citrus
PackinghousE
Citrus Pathology Day
course 3-6 pm


All events subject to change.
10-11 Crop insurance, insurance agents,
meeting/information. BHG Teaching Lab.

11 CREC Meet the Graduate Students
Symposium. 10 am 12 pm, BHG 1-2. All
personnel invited to meet CREC graduate
students and their advisors. Open to the
public. Refreshments provided.

1 pm: Grad students to meet with Dr.
Rebecca Darnell re. grad student recruit-
ment to off-campus centers, BHG 3-4
2 pm: Faculty to meet with Dr. Rebecca
Darnell re. grad student recruitment to off-
campus centers, BHG 3-4
16 CRE Foundation Board meeting, Packing-
house conference room.
17 Farmworker Citrus Canker Decontamina-
tion workshop. Distribution and instruc-
tion on the use of new citrus canker
decontamination training materials for
harvesters and grove workers. BHG 1,
9:30 a.m. 2:30 p.m.
18 Dept. of Environmental Protection work-
shop; information on citrus program for air
pollution emissions:
www.dep.state.fl. us/air/programs
citrus. htm
27 -Faculty meeting, H. Bronwing. BHG 3-4.

Class at CREC this semester:

Citrus Pathology (PLP 5115C), a graduate-
level course; Tuesdays, 3-6 p.m. (Aug. 24 -
Dec. 14); BHG Teaching Lab.

UF classes start August 23.

Upcoming:
Citrus Expo at the Lee Civic Center, Fort Myers.
August 25-26.

Citrus Packinghouse Day, Sept. 2. CREC.
Annual event for citrus packers.




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