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Group Title: Department of Animal Science research report - Florida Agricultural Experiment Station ; AL-1980-11
Title: The concomitant use of oxfendazole and trichlorfon as an equine anthelmintic
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Full Citation
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Permanent Link: http://ufdc.ufl.edu/UF00073120/00001
 Material Information
Title: The concomitant use of oxfendazole and trichlorfon as an equine anthelmintic
Series Title: Department of Animal Science research report
Physical Description: 2, 1 p. : ; 28 cm.
Language: English
Creator: Asquith, Richard L
Kulwich, Roman, 1925-
University of Florida -- Dept. of Animal Science
University of Florida -- Agricultural Experiment Station
Publisher: Florida Agricultural Experiment Station
Place of Publication: Gainesville Fla
Publication Date: 1980
 Subjects
Subject: Anthelmintics -- Research -- Florida   ( lcsh )
Horses -- Parasites -- Control -- Florida   ( lcsh )
Genre: government publication (state, provincial, terriorial, dependent)   ( marcgt )
non-fiction   ( marcgt )
 Notes
Statement of Responsibility: R.L. Asquith and R. Kulwich.
General Note: Caption title.
General Note: "September, 1980."
Funding: Animal science research report (University of Florida. Dept. of Animal Science) ;
 Record Information
Bibliographic ID: UF00073120
Volume ID: VID00001
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 80754185

Table of Contents
    Experiment
        Page 1
    Results
        Page 1
        Page 2
    Table 1 - Summary of results of anthelmintic trial
        Page 3
Full Text

Department of Animal Science ----Florida Agricultural
Research Report f-s%_- \ Experiment Station
September, 1980 Gainesville, Florida

THE CONCOMITANT USE OF OXFENDAZOLE AND TRICHLORFON

AS AN EQUINE ANTHELMINTIC

R. L. Asquith and R. Kulwich1

Both oxfendazole and trichlorfon are recognized as safe and efficacious
anthelmintics for use in the equine species and are individually approved for
clinical use. To expedite the de-worming procedure many clinicians prefer to
use two anthelmintics combined in a single dose particularly for a boticidal
effect. Information and data on the clinical safety and efficacy of these
two drugs in combination is lacking.

Experiment

The study involved 10 mares (5 Thoroughbreds and 5 Quarter Horses) be-
tween the ages of 3 and 22 years. Their weights varied from 459.9 kg to
627.3 kg. All animals were assembled, weighed, and housed in a single pad-
dock. They then were confined to individual box stalls for treatment and
collection of fecal specimens at two separate times.

The initial treatment consisted of a single dose of oxfendazole at the
recommended level (10mg/kg body weight) and trichlorfon at the recommended
level (40mg/kg body weight). The drugs were given concurrently via naso-
gastric intubation. On Day 10, the horses were treated with trichlorfon at
the recommended dose level. The animals were not fasted before either treat-
ment.

Fecal analyses (eggs per gram [EPG]) were made on Days -3 and +10, using
the McMaster Egg-Counting Chamber. All feces from each horse were collected
and screened for bot larvae for 48 hours after each treatment. Blood samples
were taken on Days -3, +1, +2, +11, +12, and +13. Plasma levels of enzymes,
iditol dehydrogenase and arginase, were evaluated to determine the possible
presence of hepatic dysfunction.

Each horse was weighed again on Day 12. Monitoring of the horses for
possible adverse clinical signs was maintained throughout the trial.

Results

No adverse clinical signs were observed during the course of this study.
Data for parasitic egg counts and bot larvae recovered are summarized in
Table 1. The total parasitic egg count (Stronaylus spp) for the 10 horses
was reduced by 99% from a mean of 458 EPG observed three days before treatment,
to a mean of 5 EPG. Only 1 horse had detectable parasite eggs on Day 10 after
receiving the combined dose of oxfendazole and trichlorfon.

No eggs other than those of Strongyus spp were observed. The 99% re-
duction in EPG for Strongylus spp after the administration of Oxfendazole and
-trichlorfon combined may be compared with results reported for the average

1Asquith, DVM & Associate Professor of Equine Health, Animal Science Department
and Kulwich, PhD, Chemist, Food Science and Human Nutrition Department,
University of Florida, Gainesville.





Department of Animal Science ----Florida Agricultural
Research Report f-s%_- \ Experiment Station
September, 1980 Gainesville, Florida

THE CONCOMITANT USE OF OXFENDAZOLE AND TRICHLORFON

AS AN EQUINE ANTHELMINTIC

R. L. Asquith and R. Kulwich1

Both oxfendazole and trichlorfon are recognized as safe and efficacious
anthelmintics for use in the equine species and are individually approved for
clinical use. To expedite the de-worming procedure many clinicians prefer to
use two anthelmintics combined in a single dose particularly for a boticidal
effect. Information and data on the clinical safety and efficacy of these
two drugs in combination is lacking.

Experiment

The study involved 10 mares (5 Thoroughbreds and 5 Quarter Horses) be-
tween the ages of 3 and 22 years. Their weights varied from 459.9 kg to
627.3 kg. All animals were assembled, weighed, and housed in a single pad-
dock. They then were confined to individual box stalls for treatment and
collection of fecal specimens at two separate times.

The initial treatment consisted of a single dose of oxfendazole at the
recommended level (10mg/kg body weight) and trichlorfon at the recommended
level (40mg/kg body weight). The drugs were given concurrently via naso-
gastric intubation. On Day 10, the horses were treated with trichlorfon at
the recommended dose level. The animals were not fasted before either treat-
ment.

Fecal analyses (eggs per gram [EPG]) were made on Days -3 and +10, using
the McMaster Egg-Counting Chamber. All feces from each horse were collected
and screened for bot larvae for 48 hours after each treatment. Blood samples
were taken on Days -3, +1, +2, +11, +12, and +13. Plasma levels of enzymes,
iditol dehydrogenase and arginase, were evaluated to determine the possible
presence of hepatic dysfunction.

Each horse was weighed again on Day 12. Monitoring of the horses for
possible adverse clinical signs was maintained throughout the trial.

Results

No adverse clinical signs were observed during the course of this study.
Data for parasitic egg counts and bot larvae recovered are summarized in
Table 1. The total parasitic egg count (Stronaylus spp) for the 10 horses
was reduced by 99% from a mean of 458 EPG observed three days before treatment,
to a mean of 5 EPG. Only 1 horse had detectable parasite eggs on Day 10 after
receiving the combined dose of oxfendazole and trichlorfon.

No eggs other than those of Strongyus spp were observed. The 99% re-
duction in EPG for Strongylus spp after the administration of Oxfendazole and
-trichlorfon combined may be compared with results reported for the average

1Asquith, DVM & Associate Professor of Equine Health, Animal Science Department
and Kulwich, PhD, Chemist, Food Science and Human Nutrition Department,
University of Florida, Gainesville.







-2-


efficacy of oxfendazole given alone at a level of 10 mg/kg in eight separate
studies. In these eight studies, average efficacies were reported as follows:
100% for S. vuloaris, 99.8% for S edentatus, and 98.6% for small strongyles.

The therapeutic activity of trichlorfon as a boticide when given in comb-
ination with oxfendazole was demonstrated in this study. Bot larvae were ex-
creted after the initial, combined dose was given, but no bot larvae were found
in the feces after trichlorfon alone was given 10 days later. This index of
therapeutic activity is based on examination of the total fecal output for 48
hours after treatment, even though more bot larvae may later have been excreted.
Administration of a second dose of boticide within 10 days of the first provided
a means of measuring boticidal effect.

One of these 10 mares appeared to have modestly elevated concentrations of
arginase and iditol dehydrogenase after receiving the combined anthelmintics on
Day 0 and the trichlorfon on Day 10. Concentration of 11.4 IU iditol dehydro-
genase/L plasma and concentration of 18 IU arginase/L plasma observed on Day 12
for this mare were substantially higher than the corresponding values recorded
three days before the initial treatment (1.4 IU iditol dehydrogenase/L and 5 IU
arginase/L). This suggests a very modest degree of liver response to the treat-
ment, resulting in slightly increased release of these two liver enzymes. How-
ever, by the next day, the plasma level of arginase in this mare had dropped to
well within the range observed three days before treatment (a value of 1 IU/L).
The plasma iditol dehydrogenase had dropped to 3.9 IU/L, compared to 11.4 on
the previous day.







TABLE 1 Summary of Results of Anthelmintic Trial


Both Groups
Quarter Horse Mares Thoroughbred Mares Combined

Mean Std. Mean Std. Mean Std.
#1 #2 #3 #4 #5 x Dev. #1 #2 #3 #4 #5 x Dev. x Dev.
(S) (S) (S)


Age (years) 18 20 17 22 15 18.4 +2.7 10 7 8 3 18 9.2 +5.5 13,8 +6.4

EPG before
first treatment 200 450 575 350 350 385 +139 1000 300 400 800 150 530 +356 458 +266

EPG 10 days
after first 50 0 0 0 0 10 +22 0 0 0 0 0 0 +0 5 +16
treatment

Bot larvae
recovered 2 1 1 1 0 1 +1 2 4 8 1 2 3 +3 2 +2
after first
treatment

Bot larvae
recovered 0 0 0 0 0 0 +0 0 0 0 0 0 0 +0 0 +0
after second -
treatment




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