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Group Title: Citrus Station mimeo report - Florida Citrus Experiment Station ; CES 64-10
Title: Identification of some bacteria frequently encountered during microbiological analysis of citrus juices and also water
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Permanent Link: http://ufdc.ufl.edu/UF00072422/00001
 Material Information
Title: Identification of some bacteria frequently encountered during microbiological analysis of citrus juices and also water
Series Title: Citrus Station mimeo report
Physical Description: 3 leaves : ; 28 cm.
Language: English
Creator: Patrick, Roger
Citrus Experiment Station (Lake Alfred, Fla.)
Publisher: Citrus Experiment Station
Place of Publication: Lake Alfred FL
Publication Date: 1964
 Subjects
Subject: Citrus juices -- Microbiology -- Florida   ( lcsh )
Citrus juice industry -- Florida   ( lcsh )
Genre: government publication (state, provincial, terriorial, dependent)   ( marcgt )
non-fiction   ( marcgt )
 Notes
Statement of Responsibility: Roger Patrick.
General Note: Cover title.
General Note: "June 19, 1964"--Caption.
 Record Information
Bibliographic ID: UF00072422
Volume ID: VID00001
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 75956996

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IDENTIFICATION OF SOME BACTERIA FREQUENTLY ENCOUNTERED DURING

MICROBIOLOGICAL ANALYSIS OF CITRUS JUICES AND ALSO WATER




Roger Patrick
Citrus Experiment Station
Lake Alfred, Florida


EMB Agar













Citrus Station Mimeo Report CES 64-10
June 19, 1964


IDENTIFICATION OF SOME BACTERIA FREQUENTLY ENCOUNTERED DURING
MICROBIOLOGICAL ANALYSIS OF CITRUS JUICES AND ALSO WATER

Roger Patrick
Citrus Experiment Station
Lake Alfred, Florida


This paper is concerned with the foods utilized and appearance of

some organisms that attracted the attention of the fruit processing

laboratory technicians when following through the routine necessary to

determine the microbiological status of citrus products and water used

in the processing plants. One phase of the routine analysis is to search

for coliform bacteria. This group of bacteria when grown in a liquid

medium containing lactose will produce a gas-fermentation. Some of this

fermenting medium is distributed over the surface of a solidified agar

plate containing lactose and a mixture of dyes, E.M.B. agar. The plates

are incubated at 35-37* C. and observed for 48 hours. If coliform

bacteria are present, the colonies will be recognized by certain character-

istics; it may be possible to predict the quality of the fruit that had

been processed and the conditions of sanitation in the plant.

Frequently and very possibly seasonally, a thin gray-blue growth

appears on the E.M.B. agar surfaces. This type of growth has been

observed by many of the control laboratory personnel and many questions

have been asked. Time does not always permit the investigation of such

problems immediately, about all the information the bacteriology lab-

oratory at the Citrus Experiment Station could give was that the growth

was atypical for coliform bacteria, had little bearing on the plant's

sanitary condition, and that they would not grow in orange juice.











Because of the interest in this problem later, cultures were

collected and a more detailed study was made when time permitted. The

report of that investigation was not made then, because of interest

in the subject seemed to have declined. Recently, the subject came

up for review and a report is being made now.

The organisms were allocated to two groups after a detailed study

was made. They were presented as of the genus, Aerobacter and the

genus Pseudomonas.

The Aerobacter group of three cultures was observed to have almost

consistently these characteristics: Acetoin was produced; methyl red

positive; gram negative; galactose, gas but neutral; arabinose, alkaline;

lactose, acid and gas; maltose, alkaline and gas; sucrose, neutral and

gas; raffinose, acid and gas; dextrine, starch, and salicin not hydrolyzed;

glycerol not used; mannitol and sorbitol, neutral and gas; citric acid

used as a source of carbon; uric acid used as a source of nitrogen;

H2S is produced; esculin is fermented; milk not coaguldted, slightly

acid, white rediment formed; cellulose not digested; carrot, moist thick

creamy growth; potato, thick pink growth; gelatin not liquified.

The Pseudomonas group of four cultures in general possessed these

characteristics:. Most media became green or blue-green in color as the

organisms matured. The odor was aromatic and resembled scented violets.

Gram negative; acetoin and methyl red negative; dexcrose, acid; levulose,

neutral; galactose, pellicle formed, otherwise no change; arabinose,

alkaline; lactose, alkaline; maltose, alkaline, pellicle formed; sucrose,

alkaline with pellicle; raffinose, alkaline with pellicle; dextrine,

starch, and salicin not hydrolyzed; glycerol, mannitol, and sorbitol

not changed; citric acid and uric acid not utilized; H S not formed;
2










esculin not fermented; milk not coagulated, was digested; cellulose not

used, carrot and potato, dark and slightly digested.

All cultures were rod-shaped, were considered saprophytic in nature,

and of no sanitary significance.













































Citrus Experiment Station
Lake Alfred, Florida
350-6/19/64 RP




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