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Group Title: Mimeo Report - University of Florida Everlgades Experiment Station ; EES64-18
Title: Evaluation of nematocide treatments on turfgrass in South florida
CITATION THUMBNAILS PAGE IMAGE ZOOMABLE
Full Citation
STANDARD VIEW MARC VIEW
Permanent Link: http://ufdc.ufl.edu/UF00067441/00001
 Material Information
Title: Evaluation of nematocide treatments on turfgrass in South florida
Series Title: Everglades Station Mimeo Report
Physical Description: 23 leaves. : ; 29 cm.
Language: English
Creator: Winchester, J. A
Everglades Experiment Station
Publisher: Everglades Experiment Station
Place of Publication: Belle Glade Fla
Publication Date: 1964
 Subjects
Subject: Turfgrasses -- Diseases and pests -- Control -- Florida   ( lcsh )
Nematocides -- Florida   ( lcsh )
Genre: non-fiction   ( marcgt )
 Notes
Statement of Responsibility: J. A. Winchester.
General Note: "April, 1964."
 Record Information
Bibliographic ID: UF00067441
Volume ID: VID00001
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 63663479

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Full Text





Everglades Station Mimeo Report EES64-18 1, ", 1964
EVALUATION OF NEMATOCIDE TREATMENTS ON TURFGRASSN SOUTH FLOOR
J. A. Winchester K


Summary

During 1963-64 21 commercial and experimental nematocide formulations made up
more than 105 replicated treatments which were compared for control of plant nema-
todes on 31 different turfgrass varieties. Only Zinophos and Bayer 25141 gave con-
sistently good nematode control and increased plant vigor. Neither of these material
caused injury to any grass tested. In one test an application of Bayer 25141 at 8
and l6 pounds per acre (0.18 and 0.36 pounds per 1000 square feet) maintained
Ormond Bermudagrass in a vigorous green condition for 12 months and controlled sting
nematodes for 9 months. The granular and liquid formulations of zinophos and Bayer
25141 appeared to be equally effective in all tests.


Experiments
Test No. Grass Location Nematodes
1. Ormond bermudagrass PFL sting
2. Everglades #1 bernudagrass PFL sting, ring, lance, spiral
3. Meyer Zoysia Ft. Lauderdale sheath, sheathoid
4. Bitter blue St. Augustine Delray cyst
5. Zoysia matrella Lake Worth sting, lance
6. Grass varieties test PFL sting, ring, lance, sheath
7. Phytotoxicity summary So. Fla. burrowing and root knot



Application: Liquid nematocides were mixed in water and the granular nematocides
with sand to aid in application. All were applied uniformly to the plot areas.
Immediately after the treatments in each test were applied the entire plot area was
sprinkled with the equivalent of one quarter to one-half an acre inch of water in
order to move the nematocides into the root zone.


Sampling:
Nematodes
flotation


Plots were sampled periodically with a soil tube to a depth of 4 inches.
were removed from 100 ml. aliquots of soil from each plot by the centrifuge
technique.


Experimental design:
domized block design.
in which plots were 3


All treatments were replicated at least three times in a ran-
Plots in all tests were 3 feet square except in the first test
feet wide and 6 feet long.


Maintenance: All plot areas were treated periodically with fertilizers, insecticides
and fungicides to produce optimum growth.


SThis report is a summary of the turf grass research conducted at the Plantation
Field Laboratory, Everglades Experiment Station and other areas. It does not
constitute a recommendation for the use of any nematocide without specific label
clearance.

SAssistant nematologist, University of Florida, Everglades Experiment Station,
Belle Glade, Fla.









Acknowledgements: The aid
maintaining the plot areas
to the value of this work.
in the following table.


American Cyanamid
Zinophos


Chemagro Corp.
Bayer 25141


Bayer 29492


of Dr. E. O. Burt in initiating these studies, and in
is gratefully acknowledged. His suggestions added much
The nematocides were provided by the suppliers listed


Co.
0,O-diethyl 0-2-pyrazinyl phosphorothioate
10% granular, 4 lbs. per gal. "Cynem".


o,o-diethyl o-p-(Methyl sulfinyl) phenyl phosphorothioate.
10% granular, 2,4,6 lbs. per gallon.


Dow Chemical Co.
Nellite phenyl NN'-dimethyl phosphorodiamidate.

DBCP Dibromochloropropane "Fumazon" 17.5 Ibs./gal.

Geigy Chemical Co.
G-33946 benzyl-N-methyl-dithiocarbamate. 25% e.c.


GS-10469

GS-10979

GS-12286


Diazinon AG500
Diazinon A-1619


0-0-diethyl 0- (2-isopropyl-b-methyl-4-pyrimidinyl)
phosphorothioate.


Niagara Chemical Div. FMC Corp.
Nia 9227 an organo-phosphorus compound.


Ethion


2 Ibs. per gal. e.c.


OO,0,',0' -tetraethyl S,S'methylene bisphosphorodithioate.


Pennsalt Agricultural
Penphene Tetrachloro thiophene. 2 lbs. per gal.

Shell Chemical Co.
DBCP Dibromochloropropane "Nemagon" 17.5 lbs. per gal.

SD7727 not released. 2 Ibs. per gallon.


Union Carbide Co.
U.C-17956


Virginia Carolina Chem. Co.
VC-13 0-2-4 Dichlorophenyl O,0-diethyl phosphorothioate.










Treatment Diagram. Ormond Bermudagrass Test #1. All treatments are in
pounds active ingredient per 1000 square feet.


____ ,,,--- --- ---
SD-7727 Check
2.9#

T5 T9

B-25141 B-25141 Check B-25141 B-25141 Check SD-7727
0.18# 0.36# 0.18# 0.36# 5.8#

T1 T2 T10 T7 T8 T9 T6

B25141 B25141 B25141 VC-13 Check VC-13 VC-13
o.36# o.18# o.36# 9# 6# 9#

T8 Tl T2 T4 T9 T3 T4

SD-7727 VC-13 B-25141 Check Check SD-7727 Check
2.9# 6# 0.18# 5.8#

T5 T3 T7 T9 T9 T6 T9

SD7727 VC-13 B25141 SD7727 Check Check B25141
2.9# 9# 0.36# 5.8 0.18#

T5 T4 T8 T6 T9 T10 T7

B25141 B25141 VC-13 Check SD-7727 Check B25141
0.36# 0.18# 6# 5.8# 0.36#

T2 Tl T3 T9 T6 T9 T2

B25141 Check VC-13 SD-7727 VC-13 B25141 B25141
o.18# 6# 2.9# 9 0.18# o.36#

T7 To1 T3 T5 T4 TI T8


+
E













Experimental Results


Test No. 1


Table 1. Effect of nematocide treatments on Ormond bermudagrass vigor. Treated
March 15, 1963.

,, .. .


__ etment Formulation


Bayer 25141
Bayer 25141
VC-13
VC-13
SD-7727
SD-7727
Bayer 25141
Bayer 25141
Check


10% gr.
10% gr.

5% gr.
2#/gal.
2#/gal.
2#/gal.
2#/gal.
1-


Rate/l000 sq. ft.


0.18 Ibs.
0.36 lbs.
6.0
9.0
2.9
5.8
0.18 Ibs.
0.36 Ibs.
--


1 yr.


1.8
1.3
0.5
0.3
0.3
0.0
0.8
1.8
0.1


4.8
4.5
3.0
2.0
2.8
1.0
5.0
5.0
1.6


5.0
5.0
2.5
3.0
3.5
2.0
5.0
5.0
2.5


/Vigor rating 0 to 5 for dead to very vigorous respectively.


T-


Vigor rating
1 mo. 6 mo.

















Table 2. Effect of nematocldal treatments on weight of 4 inch plugs of Ormond
bermudagrass from plots treated March 15, 1963 and sampled October 14,
1963. Test #1.

Treatment Weight in grams 1/
1. Bayer 25141 130
2. Bayer 25141 118
3. VC-13 47
4. vc-13 63
5. SD-7727 72
6. SD-7727 52
7. Bayer 25141 113
8. Bayer 25141 132
9. Check 72


-/ Weight was determined after the soil was rinsed fran the roots of the 4 inch
turfgrass plug samples. Each figure is the average of 3 replicate samples.



















Table 3.


Effect of nematocidal treatments on sting nematode count from Ormond
bermudagrass plots treated March 15, 1963. Test #1.


Nematode counts
Treatment 1 mo. 6 mos. 12 mos.

1. Bayer 25141 0 3 40
2. Bayer 25141 1 1 66
3. vc-13 17 22 32
4. VC-13 15 38 43
5. SD-7727 39 41 39
6. sD-7727 16 17 26
7. Bayer 25141 1 1 35
8. Bayer 25141 0 0 42
9. Check 42 44 24









Table 4. Effect of nematocidal treatments on Sheath Nematode counts from
Ormond bermudagrass plots treated March 15, 1963. Test #1.

Nematode counts
Treatments 1 mo. 6 mo. 12 mos.

1. Bayer 25141 39 48 50
2. Bayer 25141 32 61 43
3. VC-13 46 68 27
4. vc-13 51 87 38
5. SD-7727 48 104 76
6. SD-7727 56 75 21
7. Bayer 25141 36 42 50
8. Bayer 25141 40 56 20
9. Check 59 79 63


Table 5. Effect of Nematocidal treatments
bermudagrass plots treated March


on ring nematode counts from Ormond
15, 1963. Test #1.


Nematode counts
Treatments 1 mo. 6 mo. 12 mos.

1. Bayer 25141 13 29 17
2. Bayer 25141 10 21 37
3. VC-13 32 43 50
4. VC-13 19 30 32
5. SD-7727 42 87 29
6. SD-7727 21 41 0
7. Bayer 25141 38 25 43
8. Bayer 25141 30 38 91
9. Check 26 47 30









Table 6. Vigor rating of Everglades #1 Bermudagrass as influenced by
treatments applied on May 14, 1963. Test #2. 1/


nematocidal


Rate a.i. per Vigor Rating 2
Treatments 1000 sq. ft. 6 weeks 45weeks
1. Union Carbide 17956 0.46 Ibs. 1.5 1.6
2. Union Carbide 17956 0.92 Ibs. 3.5 3.0
3. Ethion 0.46 Ibs. 3.5 3.0
4. Ethion 0.92 Ibs. 4.5 3.3
5. Ethion 1.84 lbs. 4.5 3.6
6. Bayer 25141 1.84 lbs. 5.0 4.3
7. Bayer 25141 3.68 ibs. 4.5 5.0
8. Diazinon AG500 0.23 lbs. 4.5 3.6
9. Diazinon AG500 0.92 lbs. 4.0 2.3
10. Zinophos 0.37 lbs. 4.0 2.6
11. Zinophos 0.74 lbs. 4.5 2.3
12. Penphene 0.46 Ibs. 3.5 3.3
13. Penphene 0.92 Ibs. 4.0 2.6
14. SD7727 0.37 Ibs. 1.0 3.3
15. SD7727 0.74 lbs. 1.7 2.3
16. Nellite 0.05 Ibs. 2.0 2.6
17. Nellite 0.09 Ibs. 1.6 3.3
18. Diazinon A-1619 2.80 pts. 3.0 3.0
19. GS 33946 0.62 pts. 1.3 2.6
20. GS 10469 1.24 pts. 2.0 2.6
21. GS 10979 0.62 pts. 1.o 1.6
22. GS 12286 1.24 pts. 1.6 2.6
23. DBCP 2.00 lbs. 4.6 3.0
24. VC-13 2.80 pts. 2.6 2.0
25. Check 2.1 3.0
26. Check 2.8 3.0
27. Check 1.2 2.3
28. Check 0.8 2.0


/ Test initiated by A. A. Di Edwardo.

/ Vigor rating 0-5 for dead to very vigorous respectively. (The average of
3 replications.)









Table 7. Sting and Ring nematode counts from Everglades #i Bermudagrass as
influenced by nematocide treatments applied on May 14, 1963. Test #2.


Rate a.i./
Treatments 1000 fe. ft.
1. Union Carbide 17956 0.46 lbs.
2. Union Carbide 17956 0.92 lbs.
3. Ethion 0.46 Ibs.
4. Ethion 0.92 Ibs.
5. Ethion 1.84 Ibs.
6. Bayer 25141 1.84 Ibs.
7. Bayer 25141 3.68 Ibs.
8. Diazinon AG500 0.23 lbs.
9. Diazinon AG500 0.92 Ibs.
10. Zinophos 0.37 lbs.
11. Zinophos 0.74 Ibs.
12. Penphene 0.46 Ibs.
13. Penphene 0.92 Ibs.
14. SD 7727 0.37 Ibs.
15. SD 7727 0.74 Ibs.
16. Nellite 0.05 lbs.
17. Nellite 0.09 lbs.
18. Diazinon A-1619 2.80 pts.
19. GS 33946 0.62 pts.
20. GS 10469 1.24 pts.
21. GS 10979 0.62 pts.
22. GS 12286 1.24 pts.
23. DBCP 2.00 Ibs.
24. VC-13 2.80 pts.
25. Check
26. Check
27. Check
28. Check


C


--1---1----~-


I II i


Sting nematodes
0 6 45
wks ks wks.
41 43 166
17 26 155
30 170 110
20 73 140
26 33 158
10 6 55
33 0 0
28 23 195
45 22 155
38 25 143
35 3 175
17 95 140
15 112 90
48 108 95
48 303 238
10 48 167
17 212 145
86 28 400
17 106 250
13 85 225
62 163 261
12 75 196
3 225
55 168 300
52 70 215
-- 106 355
63 300 180
68 193 266


Ringnematodes
0 6 45
wks. wks. wks.
863 940 140
588 573 101
593 1236 72
443 693 161
617 806 98
640 160 117
886 345 121
448 525 92
528 216 88
466 700 91
615 788 136
726 1452 148
342 768 132
685 885 125
560 1028 101
386 753 179
352 558 142
643 273 167
858 822 140
758 720 128
675 863 136
503 1098 172
- 132 189
518 920 181
565 920 201
796 1155 145
555 712 176
602 1070 192









Treatment diagram. Test #2. Everglades 1r Bermudagrass treated May 14, 1963
at the Plantation Field Laboratory.

All treatment rates are given for 1000 square feet.



Check B25141 Check GS10469 Penphene GS12286
___ 3.68 lbs. 1.24 pt. .92 Ibs. 1.24 pt.
GS12286 Diazinon Penphene S-7727 GS-10797 S-7727
1.24 pt. A 1619 .92 Ibs. .74 Ibs. .62 pt. 32#/a

DBCP Ethion Ethion Check Zincrhos Ethion
2 Ibs. .46 lbs. .92 lbs. .74 Ibs. .46 lbs.

S-7727 Diazinon Ethion Zinophos Check B25141
.37 lbs. AG500 .92 1> .46 lbs. .37 lbs. 3.68 Ibs.
Diazinon VC-13 Diazinon Check VC-13 Penphene
AG500 jg. 2.8 pts. A1619 2.8 2.8 pts. .46 Ibs.
____* ______pts. _____...
Penphen Check UC-17956 DBCD S-7727 Check
.46 lbs. 0.46 Ibs. 2 Ibs. .37 lbs.

Nellite Check GS33946 Diazinon Nellite UC17956
.09 lbs. .62 pt. AG500 O lbs .05 lbs. 0.46 lbs.
S-7727 GS10797 Nellite Diazinon Zinophos UC 17956
.74 Ibs. .62 pt. .09 ibs. AG500 .23 11 .37 lbs. .92 lbs.

Zinophos EThion UC 17956 Nellite DBCP Ethion
.74 lbs. 1.84 Ibs. .92 lbs. .05 Ibs. 2.0 lbs. .92 lbs.
Nellite Penphene Check Penphene B-25141 Diazinon
.05 lbs. .92 lbs. .46 lbs. 1.84 lbs. AG500 .23

Ethion Zinophos B25141 Zinophos GS10469 Diazinon
.92 lbs. .37 lbs. 1.84 lbs. .74 Ibs. 1.24 pt. .92 lbs.

UC17956 GS10469 GS12286 S7727 Ethion S7727
.92 Ibs. 1.24 pt. 1.24 pt. .37 Ibs. 1.84 Ibs. .74 Ibs.

B25141 GS33946 GS10797 Ethion GS33946 Diazinon
1.84 lbs. .62 pt. .62 pt. 1.84 Ibs. .62 pt. 2.8 pts.

UC17956 Check B25141 VC-13 Check Nellite
.46 lbs. 3.68 lbs. 2.8 pts. .09 lbs.
W










Table 8. Spiral & Sheath nematode counts from Everglades 7I'l
influenced by nenatocide treatments applied on I.ay


Bcrmudacrass as
14, 1963.


Sting nematodes Ring nematodes
Rate a.i./ 0 6 45 0 6 45
Treatments 1000 sq. ft. wks. wks. wks. wks. wks. wks.


1. Union Carbide 17956
2. Union Carbide 17956
3. Ethion
4. Ethion
5. Ethion
6. Bayer 25141
7. Bayer 25141
8. Diazinon AG500
9. Diazinon AG500
10. Zinophos
11. Zinophos
12. Penphene
13. Penphene
14. SD 7727
15. SD 7727
16. Nellite
17. Nellite
18. Diazinon A1619
19. GS-33946
20. GS-10469
21. GS-10979
22. GS-12286
23. DBCP
24. VC-13
25. Check
26. Check
27. Check
28. Check


o.46
0.92
0.46
0.92
1.84
1.84
3.68
0.23
0.92
0.37
0.74
0.46
0.92
0.37
0.74
0.05
0.09
2.80
0.62
1.24
0.62
1.24
2.00
2.80
--
--


Ibs.
lbs.
lbs.
lbs.
Ibs.
Ibs.
lbs.
lbs.
Ibs.
Ibs.
lbs.
Ibs.
lbs.
Ibs.
lbs.
lbs.
Ibs.
pts.
pts.
pts.
pts.
pts.
lbs.
pts.


80
55
33
80
50
53
48
62
10
22
80
33
45
43
75
103
53
23
12
42
28
37
18
33
28
20
55
50


125
80
85
103
140
86
196
88
78
72
155
186
100
200
166
90
100
102
156
178
126
86
93
112
186
166
58
96


125
63
113
165
45
33
53
76
102
28
30
180
220
83
78
78
43
43
50
43
40
70
0
180
105
96
32
:73


98
101
117
69
50
32
21
96
68
70
42
101
73
90
142
92
127
120
88
96
72
95
99
120
101
92
130
107









Sheathoid nematode counts from Everglades
by nematode treatments applied on May 14,


#1 Bermudagrass as
1963.


influenced


o 6 45
Treatment Rate a.i./l000 sq. ft. vks. wks. wks.


1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.


0.46
0.92
0.46
0.92
1.84
1.84
3.68
0.23
0.92
0.37
0.74
0.46
0.92
0.37
0.74
0.05
0.09
2.80
0.62
1.24
0.62
1.24
2.00
2.80


Ibs.
Ibs.
lbs.
Ibs.
lbs.
lbs.
lbs.
lbs.
lbs.
Ibs.
Ibs.
lbs.
Ibs.
Ibs.
lbs.
lbs.
lbs.
pts.
pts.
pts.
pts.
pts.
Ibs.
pts.


Union Carbide 17956
Union Carbide 17956
Ethion
Ethion
Ethion
Bayer 25141
Bayer 25141
Diazinon AG-500
Diazinon AG-500
Zinophos
Zinophos
Penphene
Penphene
SD 7727
SD 7727
Nellite
Nellite
Diazinon A-1619
GS 33946
GS 10469
GS 10979
GS 12286
DBCP
vc-13
Check
Check
Check
Check


132
92
22
70
88
110
10
76
36
55
75
18
33
75
72
106
28
60
65
140
33
35
13
18
142
53
63
18


100
43
20
110
46
50
23
42
83
17
88
30
33
42
22
76
36
15
15
15
36
12
0
30
92
36
40
15


Table 9.


38
61
72
48
59
40
26
88
101
32
17
52
65
40
69
76
48
83
79
78
49
62
75
86
91
72
101
63










Table 10. Vigor rating and sting nematode counts from Meyer Zoysia as influenced
by nematocide treatments made on May 14, 1963. 1/

Vigor 2/ tng
rating 0 6
Treatments Rate a.i./1000 sq. ft. 6 wks. wks. wks.
1. U. C. 17956 0.46 Ibs. 3.5 35 28
2. U. C. 17956 0.92 Ibs. 3.9 45 52
3. Ethion 0.46 Ibs. 3.0 45 55
4. Ethion 0.92 lbs. 3.9 58 28
5. Ethion 1.84 lbs. 3.5 33 52
6. Bayer 25141 1.84 lbs. 4.5 33 '2
7. Bayer 25141 3.68 Ibs. 4.5 20 0
8. Diazinon AG500 0.23 Ibs. 3.9 20 17
9. Diazinon AG500 0.92 Ibs. 4.0 26 20
10. Zinophos 0.37 lbs. 3.3 28 56
11. zi pho 0.74 lbs. 4.5 35 10
12. Penphene 0.46 ibs. 2.5 35 45
13. Penphene 0.92 Ibs. 4.0 33 42
14. SD 7727 0.37 lbs. 4.1 22 42
15. SD 7727 0.74 lbs. 3.3 45 103
16. Nellite 0.05 Ibs. 3.0 36 106
17. Hellite 0.09 lbs. 3.5 17 28
18. Diazinon A1619 2.80 pts. 4.5 58 5
19. GS 33946 0.62 pts. 3.5 22 5
20. GS 10469 1.24 pts. 3.1 33 66
21. GS 10797 0.62 pts. 3.0 55 125
22. GS.12286 1.24 pts. 3.5 38 145
23. DBCP 2.00 Ibs. 4.5 30 0
24. VC-13 2.80 Ibs. 4.0 47 70
25. Check -3.6 33 35
26. Check -3.0 50 83
27. Check -3.5 47 66


l/ test initiated by A. A. Di Edwardo.

2/ vigor rating 0-5 for dead to very vigorous respectively (the average of
3 replications).











Table 11.


Lance, ring, and spiral nematode counts from Meyer Zoysia grass as
influenced by nematocide treatments applied May 14, 1963.


Lance Ring Spiral
Rate 2.1./ 0 6 "0 0 6
Treatments 1000 sq. ft. wks. wks. wks. wks. wks. wks.


U.C. 17956
U.C. 17956
Ethion
Ethion
Ethion
Bayer 25141
Bayer 25141
Diazinon AG500
Diazinon AG500
Zinophos
Zinophos
Penphene
Penphene
SD 7727
SD 7727
Nellite
Nellite
Diazinon A1619
GS 33946
GS 10469
GS 10797
GS 12286
DBCP
VC-13
Check
Check
Check


0.46
0.92
0.46
0.92
1.84
1.84
3.68
0.23
0.92
0.37
0.74
0.46
0.92
0.37
0.74
0.05
0.09
2.80
0.62
1.24
0.62
1.24
2.00
2.80


Ibs.
Ibs.
Ibs.
Ibs.
Ibs.
Ibs.
lbs.
lbs.
lbs.
lbs.
Ibs.
lbs.
lbs.
lbs.
Ibs.
Ibs.
lbs.
pts.
pts.
pts.
pts.
pts.
Ibs.
pts.


116
132
113
160
102
96
103
120
98
110
175
86
95
105
190
125
192
142
110
108
100
128
117
125
100
102
142


305
158
110
30
152
90
90
117
80
66
200
110
112
113
153
163
225
107
58
73
156
108
32
163
118
115
155


282
32
150
43
223
152
199
112
112
63
156
249
65
32
88
122
236
113
112
48
122
105
78
198
66
166
78


1548
148
618
152
1662
370
113
620
436
142
812
478
292
56
265
217
426
592
217
428
538
115
7
842
37
603
292


2
32
3
35
15
0
2
37
36
70
132
12
96
143
32
42
60
5
52

56
87
17
12
50
32
36


1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.









TABLE 12. EFFECT OF NEMATOCIDAL TREATMENTS ON BITTER BLUE
STi AUGlStINEGRASS VIGOR AND CYST NEMATODES IN
PLOTS TREATED OCTOBER 16, 1963.

Rate cyst Mmftodes
aiY/i.060 sq. ft. 6 24 6 24
Wks. Wks. Wks. Wks.

1. Zinophos 4 ec. 0.4 Ibs. 3.3 5.0 9 1
2. Zinophos 4 ec. 1.6 Ibs. 3.0 5.0 6 0
3. Bayer 25141 4 ec. 0.6 Ibs, 4.0 5.0 8 1
4. Bayer 25141 4 ec. 1.4 lbs. 3.3 5.0 7 0
5. DBCP 1.5 pts. 3.0 4.0 8 7
6. Bayer 29492 0.5 lbs. 3.3 4.5 6 0
7. Bayer 29492 1.5 Ibs. 3.3 5.0 6 1
8. Zinophos 10% 0.4 Ibs. 3.0 5.0 4 0
9. Zinophos 10% 1.6 Ibs. 3.3 5.0 8 0
10. Bayer 25141 10% 0.4 Ibs. 3.3 5.0 8 1
11. Bayer 25141 10% 1.6 Ibs. 3.0 5.0 5 0
12. Check 3.0 4.0 7 9









TABLE 13.


VIGOR RATINGS AND NEMATODE COURTS FROM ZOYSIA MATRILLA
IN LAKE WORTH, FLORIDA, TREATED ON FEBRUARY 1, 1964.


Treatment Rate Vigor 1/ Sting Nematodes
a.i./1000 sq. ft. 4 48 4
Wks. Wks. Wks. Wks.


1. Bayer 25141 4 ec. 0.4 Ibs. 2 5 27 1
2. Bayer 25141 10~ 0.4 Ibs. 3 5 35 0
3. Zinophos 4 ec. 0.4 Ibs. 3 5 20 0
4. Zinophos 10% 0.4 lbs. 3 5 28 0
5. DBCP 1.5 pts. 3 3 47 38
6. Check -- 2 2 56 47


I/ Vigor rating 0-5 for none to very high population.







EFFECT OF NEMATOCIDE TREATMENTS ON VIGOR AND STING NEMATODE
COUNTS IN TIFTON "57" BERMUDAGRASS TREATED ON JUNE 6, 1963.


Rate VigOr 1 Sting Nematodes
a.i./iOob sq. ft. 1 3 6 1 3 6
mo. mo. mo. mo. mo. mo.


1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.


Bayer 25141 10% gr.
Bayer 25141 10% gr.
Bayer 25141 10% gr
Bayer 25141 2#/gal.
Bayer 25141 2/gal.
Bayer 25141 2#/gal.
Bayer 25141 2#/gal.
DBCP 70%
DBCP 700
DECP 17.5% gr.
DBCP 17.5% gr.
sD 7727 W#/gal.
SD 7727 4#/gal.
SD 7727 10% gr.
SD 7727 10% gr.
Zinophos 41/gal.
Zinophos 4,/gal.
Zinophos 4#/gal.
VC-13
VC-13
VC-13 5% gr.
VC-13 5% gr.
Check


1/ 0-5 for dead to very vigorous.
2/ Actual numbers of sting nematode


per 100 ml. soil.


TABLE 14.


-


0,04
0,08
o.i6


408 iIbl
.16 lbs,
.32 lbs.
2 lbs.
3 Ibs.
2 lbs.
3 Ibs.
0.3 lbs.
0.5 lbs.
0.4 lbs.
0.8 lbs.
0.04 Ibs.
0.08 Ibs.
0.16 Ibs.
1/3 gal.
2/3 gal.
13.2. bs.
26.4 Ibs.
--


3.0 3.0
3.5 3.3
3.5 3.3
3.3 3:3
2i8 3.o
2.8 3.0
2.3 3.0
2.8 3.0
2.5 3.0
2.5 2.6
2.8 3.0
2.3 2.6
3.3 2.6
1.5 1.6
2.3 2.0
2.8 3.0
3.5 3.6
2.8 3.6
2.0 2.3
2.5 2.6
2.3 2.3
3.5 3.0
2.3 2.6


3.3
3.6
3.6
3.6
3.0
3t3
3.3
3.3
3.3
3.0
3.0
2.6
2.6
2.0
2.3
3.6
3.6
3.6
2.6
2.6
3.0
3.0
2.6


; ::


3
1
2
3
0
3
0
17
31
36
27
31
42
21
46
1
3
1
27
39
38
51
46








TABLE 15. NEMATODE COUNTS AND VIGOR RATINGS IN TURFGRASS VARIETIES 3 WEEKS AFTER TREATING WITH ZINOPHOS AND B-25141.

Sting Ring Lance Sheath Vigor
Zino- B- Zino- B- Zino- B- Zino- B- Zino- B-
phos Check 25141 phos Check 25141 phos Check 25141 phos Check 25141 phos Check 2514


Bahia Grasses
Argentine
Pensacola
Paraguayon
Para Texas #22
Paraguay
Common
Wilmington
Bermudagrasses
Everglades j1
Ormond
Uganda
Uganda
Tiflawn
Tiffine
Tifgreen
Tifway
Bayshore
Tifgreen
No Mow

Seaside Paspalum
Zoysia Grasses
Tennfolia
Ruglawn
Matrella
Emerald
Meyer
Meyer


80.
30
24
90
90
250
63
230
60
30
5


14 0
50 110
110 100
80 0
130 30
27 26
60 40

0 10-
11 33
o 26
10 0
41 56
50 20
20 37
90 110
27 70
29 160
19 50


0 10 75 40 38


30
100
81
70
85
14o


10
100
200
0
20
40
27

0
88
0
0
21
20
100
60
51
30
14


0 0
o 180
0 17
00 0
0 0
0 0
0 0
0 0
0 48
0 0
0 0


17 0 o


0 0 0


30 20
70 62
30 42
70 120
32 65
72 30


40 4
36 5
0 4
40 4
50 4
150 4
8 4

0 4
0 4
0 4
0 4
0 4
0 4
100 4
20 4
29 3
0 4
14 4

0 4

10 4
0 4
0 4
30 3
0 4
o 4


4 4







TABLE 15..continued..

Sting Ring Lance Sheath Vigor
Zino- B- Zino- B- Zino- B- Zino- B- Zino- B-
phos Check 25141 phos Check 25141 phos Check 25141 phos Check 25141 phos Check 25141
St. Augustinegrasses
Introduction 16 38 25 42 60 70 91 38 90 0 0 4 2 4
Fa-32 9 17 4 0 0 0 14 17 13 0 0 0 4 3 4
Floratine 11 10 1 29 62 14 0 0 7 79 0 38 4 3 4
Bitter Blue 16 18 30 38 35 50 0 0 0 0 0 0 4 3 4
Common 65 18 42 18 16 6 o 0 0 0 0 3 2 4
Centipedegrasses
Green stem 9 65 14 80 50 70 0 30 3 0 0 0 4 2 4
Common 14 35 36 9 27 50 0 0 0 0 0 0 4 2 4







TABLE 16. NEMATODE COUNTS AND VIGOR RATINGS IN TURFGRASS VARIETIES 6 WEEKS AFTER TREATING WITH ZINOPHOS AND BAYER,25141

Sting Ring Lance Sheath Vigor
Zino- B- Zino- B- Zino- B- Zino- B- Zino- B-
phos Check 25141 phos Check 25141 phos Check 25141 phos Check 25141 phos Check 25141
Bahia Grasses
Argentine 0 38 1 17 43 19 0 0 0 20 26 3 4 4 4
Pensacola 0 41 0 13 90 27 0 0 0 19 42 2 5 5 5
Paroguayan 3 65 1 47 76 21 0 0 o 29 36 4 4 4 4
Para Texas 22 0 32 1 0 0 0 0 0 0 19 14 7 5 5 5
Paraguay 1 46 1 20 36 30 0 0 0 11 40 10 4 3 4
Common 0 79 0 21 29 25 20 42 26 21 56 26 5 5 5
Wilmington 0 23 0 25 52 14 0 0 0 20 38 21 5 5 5
Bermudagrasses
Everglades 1 4 65 3 17 24 16 4 17 1 0 14 4 3 1 3
Ormond 1 110 0 20 42 8 0 0 0 0 0 0 4 1 4
Uganda 0 42 1 16 33 12 16 40 9 40 46 13 4 2 4
Uganda 0 85 1 7 11 11 2 11 1 3 17 1 4 1 4
Tiflawn 0 96 0 41 50 17 0 0 0 0 0 0 4 2 4
Tiffine 2 210 1 60 61 40 0 0 0 0 0 0 5 2 4
Tifgreen 1 54 2 26 90 35 21 75 0 14 96 3 4 2 4
Tifway 0 208 2 47 80 27 11 40 0 11 38 16 4 2 4
Bayshore 0 50 3 46 65 19 28 62 35 18 36 20 3 2 4
Tifgreen 1 36 1 38 140 39 8 16 10 13 19 6 3 3 3
No Mow 3 14 4 32 42 46 1 33 0 22 46 9 4 4 4
Seaside Paspalum 4 60 0 14 50 20 0 0 0 0 0 0 4 4 4
Zoysia Grasses
Tenurolia 1 63 1 3 35 14 10 26 14 27 25 14 3 2 2
Ruglawn 1 108 1 50 70 20 0 12 0 3 13 6 4 2 4
Matrella 2 80 0 9 70 16 3 39 9 1 46 10 3 1 3
Emerald 0 65 1 5 99 1 15 28 10 3 13 5 3 2 3
Meyer 1 61 1 42 60 13 14 55 20 9 27 11 4 1 4
Meyer 1 170 1 10 48 18 17 42 14 2 43 16 3 1 3







TABLE 16..continued..

Sting Ring Lance Sheath Vigor
Zino- B- Zino- B- Zino- B- Zino- B- Zino- B-
phos Check 25141 phos Check 25141 phos Check 25141 phos Check 25141 phos Check 25141

St. Augustinegrasses
Introduction 3 46 1 22 76 14 0 0 0 0 0 0 4 2 4
Fa-32 1 16 2 10 16 15 0 0 0 0 0 0 3 3 3
Floratine 2 27 1 1 13 2 40 70 20 26 60 14 4 4 4
Bitter Blue 0 19 0 38 47 6 0 0 0 0 0 0 4 4 4
Common 2 18 0 4 7 1 0 0 0 19 38 14 3 2 5

Centipede Grasses
Green stem 18 39 5 5 65 0 3 36 6 0 0 0 4 2 4
Common 20 20 10 9 38 4 0 0 0 0 0 0 3 2 4








PHYTOTOXICITY STUDY

TABLE 17. PLANTS TREATED WITH ZINOPHOS AND BAYER 25141 AT 3/4 LB. PER
SQ. FT. AS A DRENCH. ADDITIONAL WATER WAS APPLIED TO RINSE
THE MATERIAL OFF THE PLANTS AND INTO THE SOIL. NO
PHYTCTOXICITY RESULTED.


Aralia Sieboldi
Aloe Sp
Agavie americanum
Bougainvillea glabra
Carissa grandiflora
Coleus blumei
Codiaeum variegatum
Draceana fragrans
D. Samderoama
Diffenbachia sp
Ficus elastica var. decora
Gardenia jasminoides
Hibiscus rosa-sinensis
Ixora coccinea
Philodendron panduraeforms
P. cordatum
P. hastatum
P. hastatum var. Bergundy
P. pothos (marble queen)
Sanseviera hohni
S. laurentii
S. parva
Schefflera actinophylla
Peperonia
Varigated nephytis
Pineapple var. Smooth Cayenne
Pineapple var. red Spanish
Neanthe bella palm.








SUMMARY OF NEMATODE PATHOGENICITY STUDIES ON TURF GRASSES
CONDUCTED AT THE EVERGLADES EXPERIMENT STATION IN 1962-63.


Sting Lance Root Knot Cyst
Nemas Vigor Nemas Vigor Nemas Vigor Nemas Vigor
2 V


1. Ormond bermuda-
grass
2. Ormond bermuda-
grass check
3. Tifway bermuda-
grass
4. Tifway bermuda-
grass check
5. Tifgreen
bermudagrass
6. Tifgreen bermu-
dagrass check
7. Uganda
8. Uganda check
9. Tiflawn
bermudagrass
10. Tiflawn bermu-
dagrass check
11. Meyer Zoysia
12. Meyer Zoysia
Check
13. Emerald Zoysia
14. Emerald Zoysia
Check
15. Bitter Blue St.
Augustinegrass
16. Bitter Blue St.
Augustinegrass
check
17. Floratine St.
Augustinegrass
18. Floratine St.
Augustinegrass
Check
19. Centipedegrass,
Common
20. Centipedegrass,
Common, Check


2 99 4 10 3 0 5


5 0 5


3 142


5 0 5


3 110


5 o


o 5

7 4


0 5

0 5


0 5 o 5


4 13 4 0 5

5 0 5 0 5


113
0
148


5 o 5


137
0

55
0


2 29


5 0


2 0


50 2


0 5 0 5


0
- 0


5 23 3 38 1

5 0 5 0 5

0 19 2 44 2


0 5 0 5


- 25 3 0 5


0 5 0 5


i/ The number of nematode in 100
months after inoculating with
root knot nematode egg masses
of 4 replications.
/ Vigor rated from 1-5 for dead
SRoot knot adults on 5 grams o:
EE c64-18
600 copies


ml. of soil from each pot approximately 8
800 sting nematode, 750 lance nematode, 10
or 50 cysts respectively. All are averages


to very vigorous, respectively.
f grass roots.


TABLE 18.




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