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April May 2002 Entomology and Nematology News
Entomology and Nematology Student Organization
A University of Florida Publication
Meetings and Publications
James P. Cuda was invited by Steve Shaw, Vice-President of the Southwest Chapter of the Florida
Association of Environmental Professionals, to give a presentation at their March meeting in Ft. Myers, on
biological control of weeds focusing on melaleuca. The title of the presentation was "Classical Biological
Control of Weeds in Florida: The Melaleuca Weevil Success Story." The first part of the presentation
introduced basic concepts on biological control of weeds, and provided an overview of Florida's programs.
The second part of the presentation focused on melaleuca, a highly invasive weed in southwest Florida.
Various aspects of the biology the Australian weevil Oxvops vitiosa, a recently introduced natural enemy
of melaleuca, and techniques for redistributing the insect to melaleuca infested sites were presented.
Eileen Buss, landscape and turf extension entomologist, recently used a PowerPoint presentation in a talk
at the SE Pest Management Conference called "Challenges of Insect Pest Management in the Landscape"
and was asked by several county faculty to post it to the UF/IFAS Presentations Web site. It is there now.
Elizabeth Felter of Orange County Extension has also contributed a PowerPoint presentation titled
"Scouting A Real Life Experience." The UF/IFAS Presentations Web site can be accessed through htp://
pests.ifas.ufl.edu/ To access this site requires a IFASDOM username and password. If you don't have one
or can't remember it, then you must contact IFAS IT.
Jim Cuda and Norm Leppla also posted the following PowerPoint presentation on the UF/IFAS
Presentations Web site: "Lovebugs in Florida Setting the Record Straight."
Norm Leppla recently traveled to Tunis, Tunisia and visited the Institute National Agronomique de
Tunisie, Departement de Biologie to review a research project for the International Atomic Energy
Agency, Food and Agriculture Organization of the United Nations, "Control of Date Moth Using Radiation
Sterilization." The objectives of this assignment were to assist the counterpart, Professor Dr. Mohamed
Habib Dhouibi, leader of the date moth eradication project, in improving rearing of the date moth (carob
moth), Ectomyelois ceratoniae (Lepidoptera: Pyralidae), with special emphasis on mating densities in
oviposition cages, mating procedures, diet preparation, marking with calico red dye, irradiation for sexual
sterilization, and associated procedures. Additionally, a meeting was held with Professor Mougou
Abdelaziz, President, Institution for Agriculture Research and Higher Education, Ministry of Agriculture to
determine if an irradiator could be housed in the date moth rearing facility. The mission was successful in
determining the status of the date moth project, including capabilities of the staff, resource requirements,
pilot level operations, and support from the Tunisian Ministry of Agriculture. Professor Dhouibi is
exceptionally capable and productive, and has the full support of his administration. Although functional,
the rearing system could be improved in several ways to help prevent problems in the future. It is notable
that the colony was appropriately established with insects from the target population and has been in
production for about 25 generations. Irradiation is a bottleneck to releasing the maximum number of moths
that can be reared; however, there are several options for solving this problem. Success in the field will
depend on reducing the size of the target population by site selection and cultural practices in date
production. It is essential to devise an efficient means of determining and tracking levels of sterility in wild-
type date moths. As the eradication project becomes operational, the date moth will have to be excluded
from areas where it has been eradicated.
PCT Magazine, a national magazine servicing the urban pest control industry, contained an article in its
March 2002 issue titled "Insect Repellents Provide Safe Relief With Proper Use." The article is designed to
be torn out of the magazine so that companies can reproduce it, stamp their company name and address on
the top and distribute to customers. The article was written by Angela Brammer, a M.S. student in our
department. Angela has a B.S. in Journalism (with emphasis on copy editing) and intends to be a science
writer. She is currently working on the final review of the new national Public Health Pest Control
Pesticide Applicator manual, of which she is one of the editors. A link to the online version of Angela's
article in PCT magazine is available from the Florida Pest Alert site. The Florida Pest Alert WWW site is
available at http://PestAlert,ifas.ufl.edu/
Levy, H. C., Garcia-Maruniak, A. and Maruniak, J. (2002). Strain identification of Spodoptera
frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of Citochrome Oxidase C subunit I
gene. Florida Entomologist 85(1): 186-190.
Russell Mizell, Thomas Fasulo and Donald Short released version 2.0 of the WoodyBug CD-ROM.
WoodyBug 2.0 contains new and updated information and photographs. However, whereas WoodyBug 1.0
only ran on Windows PCs, WoodyBug 2.0 runs on any Mac or Windows PC with a CD-ROM drive, a
mouse and a World Wide Web browser. WoodyBug 2.0 (SW-119) contains detailed information on pest
and beneficial arthropods of woody ornamentals in the southeast United States. There are over 170 color
images, including over 150 color photographs of the arthropods (mostly insects). The CD-ROM is heavily
oriented toward IPM, and also contains detailed information on beneficial arthropods. At least 70 color
images are on the beneficial insects and mites. Other areas covered, besides in-depth information on pest
groups, include host plant resistance, biologically compatible pesticides, scouting and monitoring. For
details access the UF Buggy Software Web site at http://pests.ifas.ufl.edu/software/.
James Cuda, Norman Leppla and Eileen Buss conducted an in-service training program for county
faculty using interactive videoconferencing technology on April 10th. The half day distance education
workshop on "Integrated Pest Management Emphasizing Biological Control in Florida's Agricultural,
Urban and Natural Environments" originated from the Gainesville Distance Education Unit in McCarty
Hall, and was transmitted to remote sites in Fort Lauderdale, Immokalee, Quincy, Fort Pierce, Homestead,
Lake Alfred, Apopka and Bradenton. Approximately 60 individuals at the aforementioned locations
participated in the workshop that included presentations by James Cuda, Norm Leppla, and Eileen Buss
as well as Donald Dickson, Raghavan Charudattan, Martin Adjei, Howard Frank, Lance Osborne,
Gregg Nuessly, Phil Koehler, and Freddie Johnson. The goal of the workshop was to increase
communication among state specialists and county faculty around the state who are interested in IPM
emphasizing biological control.
Hazel C. Levy, Alejandra Garcia-Maruniak and James E.Maruniak.2002. Strain identification of
Spodopterafrugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytochrome oxidase
C subunit I gene. Florida Entomologist 85: 186-190.
James Cuda was named Associate Editor of BioControl (formerly Entomophaga), the official journal of
the International Organization for Biological Control. Cuda will be responsible for soliciting reviews on
papers in the sub-discipline of weed biological control.
Minor, R.K., T.R. Fasulo, and A.D. Koehler. Gentrol IGR A computer- verified training\CEU tutorial.
SW-152. March 2002. 11Megabytes.
James Cuda was a guest lecturer for ENY 6203 (Insect Ecology) on April 17th. Cuda presented a lecturer
on aquatic insect communities using examples from his research on biological control of the aquatic weed
Dr. James Cuda participated in an in-service workshop for extension specialists to learn how to develop
and conduct more effective in-service training programs for county faculty, and also how to improve
writing skills. This one-day workshop was held in Gainesville, April 23rd.
Norm Leppla An on-site review of the Insect Production Unit (IPU), Great Lakes Forestry Centre, Sault
Ste. Marie, Ontario was just completed. The review was conducted at the direction and under the
leadership of Errol Caldwell, Director, Integrated Pest Management, Great Lakes Forestry Centre (GLFC).
The review team (Norm Leppla, Brian Melin, Fred Stewart, and Jay Whistlecraft) was asked to "critically
examine our rearing facility and rearing practices with particular attention to issues affecting quality
control," specifically to "eliminate current microbial contamination in our budworm colony, which steps
need to be taken to prevent re-infection, and what we need to change in our rearing practices, facility, and
QC management in order to maintain high-quality and disease-free rearing stocks in the longer term." The
IPU currently produces 12 species of forest insects and two species of other interest. Spruce budworm
(SBW) constitutes 75% of production with about 3.5 million larvae per year distributed to a wide range of
important research projects. The IPU is staffed by four permanent full-time and two temporary employees
with periodic seasonal assistance. A reliable source of high quality, normal (diapausing) SBW is urgently
needed to support planned research projects to manage this forest and urban pest. The second stage SBW
larvae are held in diapause for about six months before completing development, so production must be
stabilized almost a year before the insects will be available for seasonal research. Consequently, the review
team divided its findings into two general categories: immediate action to solve the SBW rearing problems
and supportive activities for the IPU. Immediate action included treating the SBW colony infection,
establishing a disease-free colony of diapausing SBW, isolating rearing facilities from sources of
contamination, instituting procedures to assure a healthy colony, and using QC procedures to immediately
detect and correct rearing problems. Supportive activities were classified into the following categories:
staffing; training and education; resources; focus and prioritization; and communication and consultation.
A comprehensive report is available.
On April 12, in London, Tom Walker spoke at the 18th International Learned Journals Seminar,
sponsored by the Association of Learned and Professional Society Publishers. This year's seminar was
titled "We can't go on like this: the future ofjournals." Walker's talk, "Author (or sponsor) pays," was one
of four talks in a session on alternative business models. In it, he described how two journal-publishing
societies (Florida Entomological Society and Entomological Society of America) were making money by
providing (at a fair price) what their authors want--namely, immediate free Web access (by everyone) to
the authors' articles. The seminar was attended by representatives of the major commercial publishers of
journals as well as by those from society-based publishers. Should anyone be interested in the details of the
talk, it can be viewed or downloaded at http://csssrvr.entnem.ufl.edu/-walker/epub/ALPSP.htm.
Eileen Buss recently attended the IR-4 meeting for minor use crops in Houston, TX (April 14-16). She met
with other research and government entomologists, product manufacturers, and ornamental growers to
discuss the prioritization of phytotoxicity and efficacy studies of insecticides and miticides currently
registered through EPA and those in the "pipeline." The ultimate goal of the IR-4 program is to assist in
obtaining data for pesticide and biopesticide label registrations and label expansions.
Kathryn Barbara and Eileen Buss conducted a field demonstration of the entomopathogenic nematode
that attacks exotic mole crickets, Steinernema scapterisci (Nematac S), in Bradenton on April 5th. About
60 school administrators, county agents, master gardeners, teachers, students, a school principal, school
grounds crews, parks and recreation personnel, pest management professionals, distributors, and golf
course superintendents attended. Students in one of the science classes were enthusiastic about helping
with the research/demonstration project. Even some pest management professionals, who have resisted
adopting an IPM strategy, were really interested in working with the nematodes and other mole cricket
biological control agents.
Speakers for the 2002 Southeast Pest Management Conference held May 5-8 at the Constans Theater
included, Eileen Buss, Billy Crow, Philip Koehler, Faith Oi, Brian Cabrera, Matt Aubuchon, Deanna
Branscome, Shawn Brooks, Rebecca Baldwin, Katie Barbara, Roxanne Burrus, Cara Congdon,
Brian Eisenberg, Richard Martyniak, Kim McCanless, Robin Minor, Dina Richman, and Cindy
Eileen Buss was recently awarded $67,000 in a three year EPA Strategic Agricultural Initiative Grant,
titled "Evaluation of Integrated Pest Management Practices in Urban Turfgrass."
Scotty Long was the recipient of the Outstanding Graduate Student Paper Award in the Agricultural and
Natural Resources Section of the Florida Academy of Sciences. The award was in recognition for Scotty's
oral presentation, "Initial Testing Results of a Novel Bio-Rational Compound for the Control of Insect
Pests of Agricultural and Medical Importance" at the Academy's 2002 Annual Meeting held at Barry
University, Miami, 7-9 March. Lucy Treadwell also received the Honorable Mention Graduate Student
Paper Award in the same section for her presentation titled, "Population Dynamics of Meristems Among
Defoliated Brazilian Peppertrees: Consequences for Growth and Reproduction."
Several students in the Entomology and Nematology Department were recently recognized by Charles E.
Young, President of the University of Florida, "for outstanding achievements and contribution to the
University of Florida." The recipients were Cynthia Khoo, Heather Smith, J. Chapman, Alison Neeley,
Scotty Long, Jim Dunford, Rebecca Baldwin, Wade Davidson, and Kelly Sims.
In April, Emily Heffernan, a student of Tom Emmel, received a Fulbright Fellowship Award to
Malaysia. She will be doing fieldwork out of FRIM (The Forest Research Institute of Malaysia) for ten
months. The grant totals $17,800 and includes travel, living, research costs, and a monthly stipend. Her
research will consist of fieldwork on symbiotic ants and lycaenid caterpillars in the 100 million year old
rainforests of western peninsular Malaysia.
Cynthia Khoo, a Ph.D. student in the lab of Pauline Lawrence, was recently inducted into the Gamma
Sigma Delta honor society of Agriculture. She was also recognized as one of five outstanding International
graduate students in the College of Agricultural and Life Sciences. Students were selected based on
"academic success (GPA, scholarly activity, and outstanding service to their department, college or the
University). In December 2001 Cynthia was elected as a member-at-large of the International Affairs
Committee, a Standing Committee of the Entomological Society of America. Congratulations for a job
well done, Cynthia.
In recognition of her academic achievements, Kathryn Barbara, who works in the Landscape
Entomology program, was inducted into Gamma Sigma Delta in April 2002. Kathryn also recently
received the Vam York Agricultural Women's Scholarship as a result of her hard work, academic
accomplishments, community involvement, and professional goals.
Jennifer Lanoie graduated this spring with a 4.0 and highest honors for her B.S. She did her Honors
Thesis with Dr. James Maruniak. The title of her Thesis is "Sequencing and bioinformatics of the
Neodiprion sertifer nucleopolyhedrovirus."
Debbie Hall and Jerry Wenzel have been awarded the Superior Accomplishment Award for their service
to our department. Congratulations.
James Cuda was awarded a $2,400 grant from the Office of the Dean for Research. The grant is being
used to support Bobbie Jo Davis, a summer intern who will be working on a project to develop an
artificial diet for rearing the flower bud weevil Anthonomus tenebrosus, a candidate for biological control
of tropical soda apple.
Congratulations to the new ENSO and UES officers for the 2002- 2003 school year. The ENSO officers
are President, Trevor Smith, Vice President, Alejandro Arevalo, Secretary, Cara Congdon, Treasurer,
Cindy Tucker, and Historian Rebecca Baldwin. UES officers are, President, Ricky Vasquez, Vice
President, Joe Jonovich, Secretary, Cara Congdon, Treasurer, Cindy Tucker, and Historian, Richard
The Extension Computer Lab has been busy the last two months generating seven new CDs and working
on more. As a result, only two new Creatures were added in February and March and they were previously
reported in this newsletter. However, some of the text and images in the Featured Creatures lady beetles
file have been changed. Thanks go to J.P Michaud for providing positive identification of Olla v-nigrum
Casey and Chilocorus stigma (Walker) and images. So this issue will be used to provide the correct
citation for Web-based articles. In a recent listing of publications by departmental faculty, I observed that
almost everyone who contributed a Featured Creatures used a different form of citation. The correct
citation is based on The Columbia Guide to Online Style Web site at http://www.columbia.edu/cu/cup/
To quote from the site in regard to World Wide Web citations:
"Scientific Style: Give the author's last name and initials (if known) and the date of publication in
parentheses. Next, list the full title of the work, capitalizing only the first word and any proper nouns; the
title of the complete work or site (if applicable) in italics, again capitalizing only the first word and any
proper nouns; any version or file numbers, enclosed in parentheses; the protocol and address, including the
path or directories necessary to access the document; and finally the date accessed, enclosed in
Burka, L. P. (1993). A hypertext history of multi-user dimensions. MUD history. http://www.utopia.com/
talent/lpb/muddex/essay (2 Aug. 1996).
However, if you are the author listing your own work, then the final date is not necessary. This applies to
people citing your work to show the date they accessed it. After all, Web sites can be changed and what
you have written on it today you could change tomorrow.
As an example, an author would list his or her Featured Creatures as:
Brammer, A., and W.T. Crow. (December 2001). Red ring nematode, Bursaphelenchus cocophilus
(Cobb) Baujard. UF/IFAS Featured Creatures. EENY-236. http://creatures.ifas.ufl.edu/nematode/
red ring nematode.htm
Featured Creatures Kudos
"Thank you for a comprehensive and organised website. It is one of the best ones for pests that I have seen
yet!" Emma Lumb, Office of the Chief Plant Protection Officer, Department of Agriculture, Fisheries &
Florida Today, "The Space Coast's Newspaper" of Brevard and adjoining counties, ran an article on
beneficial insects and listed five files from Featured Creatures as sources. These files were on brown
lacewings, lady beetles, ringlegged earwigs and two parasitic wasps, Cotesia marginiventris and Diadegma
With money from the Office of the Provost and from the Dean of the College of Agriculture and Life
Sciences, an academic pavilion will be built in the Natural Area Teaching Laboratory. The pavilion will
provide shelter from rain and lightning for classes of up to 38 students. It will be located near the kiosk and
will have tables with bench seating to facilitate students working with specimens and taking notes.
Construction of the pavilion will be supervised by IFAS Facilities Operations.
New insect order to be reported in Science http://news.nationalgeographic.com/
news/2002/03/0328 0328 TVstickinsect.html
The Florida Department of Health Weekly Arbovirus Summaries are now available from the Florida Pest
Alert site at http://PestAlert,ifas.ufl.edu/. You can also access the 5-page Florida Arbovirus Response Plan
2002 from the Pest Alert site. This is a 66 KB PDF file.
With dengue fever again a serious problem in South America and as close to the USA as Cuba and the
Caribbean, the Florida Department of Health has enhanced its surveillance for dengue fever in this state.
Details are available on the Florida Pest Alert site.
Daniel Sonke, extension graduate assistant for the Florida IPM and Biological Control Program, took a
day out of his spring break to take an IFAS Communication Services video crew to the Bradenton area to
film an integrated pest management success story in Manatee County. IFAS extension specialists were able
to convince a large corporate tomato grower to switch to IPM methods by scouting a sample field for just
one season. Now the grower feels that IPM is a necessary part of his tomato production. Sonke arranged
for the success story to be filmed by ICS. It will be made into a TV news article which will be released to
Florida and nationwide news services.
Entomology and Nematology Course Announcement: ENY 6822C Molecular Biology Techniques
Summer A 2002 (May 13th to June 21st). This will be a hands on laboratory course. It targets Entomology
and Nematology graduate students interested in learning, understanding and applying molecular biology
techniques for their own research projects. The students will extract the DNA that will be used for further
experiments. Maintenance of an adequate laboratory notebook, oral presentation of a project using the
techniques learned, attendance and active participation in every aspect of the course will be the major
criteria for grades. Instructors: James Maruniak (ext 148) and Alejandra Garcia Maruniak (ext 203)
The University of Florida has updated its Label Tutorials to reflect the latest issue of the labels and has
also converted the tutorials from a diskette format to CD- ROMs. The three Label Tutorials that were
updated were Premise 75, Siege gel and Talstar F. We have also issued a new Label Tutorial on Gentrol
IGR. Each of these tutorials is available for one CEU in Florida for Core/General Standards in numerous
categories. Premise 75, Siege gel and Talstar F are also certified for several categories in the State of West
Virginia. The cost of the tutorials is $15 each. Receive a 25% discount when ordering in quantities of 25 or
more (the Label Tutorials can be mixed to receive the discount). A 40% discount is available to resellers
for quantities of 10 or more. Details are on the UF Buggy Software Web site at http://pests.ifas.ufl.edu/
The University of Florida Core2 (Pesticide Labeling) and Core6 (Emergency Response to Harmful Effects
of Pesticides, Heat Stress and Heat Stroke) Tutorials are now available on CD-ROMs. In addition, the price
of each tutorial is reduced from $35 to $25. Quantity discounts are available. Purchase 25 or more copies
and receive a discount of 25%. Resellers can receive a discount of 40% on orders of 10 or more. These
tutorials are based on Chapters 2 and 6 of the USDA Applying Pesticides Correctly manual. Core2 and
Core6 are each authorized for two CEUs in Florida and West Virginia for pesticide applicator
recertification in numerous categories. Detailed information on the CDs is available on the University of
Florida Buggy Software Web site
The State of Arizona Structural Pest Control Commission has approved 16 of the University of Florida
computer-verified training tutorials for CEUs and recertification of pesticide applicator licenses in nine
categories. These tutorials are developed by Tom Fasulo and other members of the department. For details
see the University of Florida Buggy Software site.
The State of Vermont Department of Agriculture, Food and Markets has approved 20 of the University of
Florida computer-verified training tutorials for CEUs and recertification of pesticide applicator licenses in
11 categories. These tutorials are developed by Tom Fasulo and other members of the department. For
details see the University of Florida Buggy Software site.
SOMETHING MISSING FROM YOUR NEWSLETTER?
If there is something you would like to see in future editions of the newsletter, pleas send all thoughts,
suggestions and supportive criticisms to Rebecca Baldwin.
Special thanks to Tom Fasulo who maintains the WWW site for the newsletter and to Pam Howell who is
a tremendous help in the editing of the newsletter.
A hard copy of this newsletter is given to department members in building 970 only. All others can obtain
an electronic subscription by sending a request to listserv(@lists.ufl.edu and in the text of the message type:
subscribe UF-bugnews-L yourfirstname yourlastname
Turn off any signature file, if you have one. You will receive instructions for confirming your subscription
and further information on the rules for the list server.
This version of the newsletter is prepared for the Web by Andy Koehler.
May 2002. Updated July 2003.
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