Group Title: Research report (North Florida Research and Education Center (Quincy, Fla.))
Title: Evaluation of a new biologically-derived nematicide, ABG 9017, to manage nematodes in tobacco
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 Material Information
Title: Evaluation of a new biologically-derived nematicide, ABG 9017, to manage nematodes in tobacco
Series Title: Research report (North Florida Research and Education Center (Quincy, Fla.))
Physical Description: 5 leaves : ; 28 cm.
Language: English
Creator: Rich, J. R ( Jimmy Ray ), 1950-
Whitty, E. B
North Florida Research and Education Center (Quincy, Fla.)
Publisher: North Florida Research and Education Center
Place of Publication: Quincy Fla
Publication Date: 1996
Subject: Tobacco -- Effect of pesticides on   ( lcsh )
Nematocides   ( lcsh )
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
Bibliography: Includes bibliographical references.
Statement of Responsibility: J. R. Rich and E. B. Whitty.
General Note: Caption title.
 Record Information
Bibliographic ID: UF00066139
Volume ID: VID00001
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 71196028

Full Text

:r 9 191996
Uni'ersiti of forida


IFAS NFREC Research Report 96: 10

J. R. Rich
University of Florida
Route 3, Box 4370
Quincy, FI 32351

E. B. Whitty
University of Florida
304 Newell Hall
Gainesville, FL 32611

~3 ~,M)

Abbott Tobacco Nematicide Test 1995

The trial reported herein was conducted to evaluate the effectiveness of a new

nematicide, ABG 9017, from Abbott Laboratories to control Meloidogyne javanica on

flue-cured tobacco.

Materials and Methods

A field trial was conducted on a fine sand soil (93% sand, 4% silt, and 3% clay)

infested with Meloidogyne spp. (primarily M. javanica). In 1994, the site was

previously in tobacco. In early April, the field was moldboard plowed and disced.

Before tobacco transplanting, the plot area was double-disced and herbicide application

was made at that time. On April 11, treatment with Nemacur 3 SC was made and on

April 12, the ABG 9017 was applied. All treatments were applied with a CO2 backpack

sprayer, with Nemacur 3 SC broadcast in 20 gallons of water per acre, and the ABG

9017, 0.10" of overhead irrigation was applied, and the next day, 0.25" was added.

Tobacco cv. Hicks was transplanted 18" apart in-row on April 14. The single

row plots were 48 inches wide and 30 ft. long. The ABG 9017 treatments were

arranged with treated and untreated rows for paired plot comparisons, while the control

and Nemacur treatments were two-row plots. Two additional treatments were made to

one of the 50 lbs. a.i./A ABG 9017 band treatments. A 50 lb. a. i./A, 24" band

treatment was made on May 22, 41 days after the first treatment. Irrigation of 0.25"

followed the treatment after 48 hours. The third and same treatment was made on June

23, 73 days after the first treatment and irrigated immediately with 0.25" of water.

Plants were observed for phytoxotcity and harvested 3 times over the season.

Soil samples were taken on June 26 by removing 6 soil cores per row to a depth of 10".

Samples from individual plots were combined and 100 cm3 extracted by the modified

centrifugation-flotation technique. Root gall ratings (0-4 scale) were taken 2 days after

final harvest from 4 plants in each plot on August 2, 1995. Tobacco was harvested

three times over the season and cured weights recorded.


No phytotoxicity was found in any of the preplant treatments or in the first post-

plant treatment of ABG 9017. However, in the second post-plant treatment, minor leaf

necrosis was evident where the ABG 9017 had been sprayed. Few differences in

nematode numbers were found among treatments and numbers were low at the mid-

season sampling. Paired plot comparisons indicated lowest root galling in the 100 lbs.

a.i./A band treatment with ABG 9017 followed by the Nemacur 3 SC treatment. When

the experiment was treated as a completely randomized block design (east row

comparisons), similar trends of lowest root galling was observed with these two

treatments. Plant growth in plots was good but stands were highly erratic. Yield data

were thus discarded in this test.


Both the ABG 9017 and Nemacur 3 SC reduced root galling compared to the

control treatments. Data were highly variable especially for yield of tobacco due to

"patchiness" of nematode distribution in the test area and greatly differing number of

plants in each plot. A heavy infestation of Ridomil-resistant blue mold reduced number

of plants in random plots. Root galling data, however, indicated consistent activity of

the ABG 9017 in reducing this parameter of nematode infection in tobacco.


Table 1. Paired plot comparionss of ABG 9017 treated and untreated tobacco root gall
ratings, 1995.1

ABG 9017

Rate in
lbs. a.i./A

Nemacur 3 SC

ABG 9017

ABG 90174

ABG 9017

ABG 9017

50 + 50 + 50








Control -- 2.45 (U)

1Predominantly Meloidogyne javanica with some M. incognita.

2All band applications were 24" wide; BCI indicates broadcast and incorporated 4-5"
deep; BC represents broadcast followed by light irrigation.

3Root galling based on a 0-4 scale using 4 plants per plot.

4Treatment applications were made preplant, 41 days and 73 days afterwards.

1.25 (T)
2.21 (U)
1.54 (T)

1.55 (T)
2.17 (U)
1.69 (T)
2.00 (U)
2.05 (T)
2.00 (U)
2.05 (T)
1.55 (U)

Table 2. Influence of ABG 9017 on root galling and nematode numbers in a tobacco
trial, 1995.1,2
Rate in Application Root No. nematodes/
Treatment3 lbs. a.i./A. method3 Galling5 100 cm3 soil

ABG 9017 100 Band 1.25 89

ABG 9017 50 Band 1.55 104

ABG 90174 50+50+50 BC+Band+Band 1.69 72

Nemacur 3 SC 6.0 BCI 1.75 48

ABG 9017 100 BC 2.05 131

ABG 9017 50 BC 2.05 115

Control -- 2.57 87

'Predominantly Meloidogyne javanica with some M. incognita.

2Treated as a randomize complete block design using east row from all treatments.

3Band applications were 24" wide, BCI represents broadcast and incorporated 4-5"
deep, BC is broadcast followed by light irrigation.

4Treatment applications were made preplant, and 41 days and 73 days afterwards.

SRoot galling based on a 0-4 scale using 4 plants per plot.

. I 4

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