Group Title: Research report (North Florida Research and Education Center (Quincy, Fla.))
Title: Investigation of soybean stress from defoliating pests Alabama and Arkansas
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 Material Information
Title: Investigation of soybean stress from defoliating pests Alabama and Arkansas
Series Title: Research report (North Florida Research and Education Center (Quincy, Fla.))
Physical Description: 15 pages : ill. ; 28 cm.
Language: English
Creator: Mueller, A. J ( Arthur John ), 1932-
North Florida Research and Education Center (Quincy, Fla.)
Publisher: North Florida Research and Education Center
Place of Publication: Quincy Fla
Publication Date: 1992
 Subjects
Subject: Soybean -- Effect of pesticides on   ( lcsh )
Soybean -- Alabama   ( lcsh )
Soybean -- Arkansas   ( lcsh )
Genre: non-fiction   ( marcgt )
 Notes
Statement of Responsibility: A.J. Mueller ... et al..
General Note: Cover title.
 Record Information
Bibliographic ID: UF00066097
Volume ID: VID00001
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 71171831

Full Text
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University of Florida

Investigation of Soybean Stress from Defoliating Pests:
Alabama and Arkansas
A. J. Mueller, T. P. Mack, L. G. Higley, J. E. Funderburk and I. D. Teare































North Florida Res. and Educ. Ctr., Univ. of Fla., Quincy, Fla. 32351; ; Dept.
of Entomol., Univ. of Arkansas, Fayetteville, AR 72701; Dept. of Zoology, Univ.
of Alabama, Auburn Univ., Auburn Univ, AL 36849; Univ. of Nebraska, Lincoln, NE
68583. Research NF-92-4.








INTRODUCTION
Defoliation by insect pests is a major stress of soybean in the Southern

Region. Quantifying physiological responses of soybean to defoliation is

essential for understanding how insect defoliation reduces soybean yields.

Indeed, defoliators comprise the most abundant and diverse guild of insects that

attack soybean in the U.S. (Turnipseed and Kogan 1976). Understanding the

relationship between defoliation and plant responses, such as yield is essential

for better pest management.

The objectives of this study are: 1) To characterize effects of simulated

insect defoliation on soybean physiology, 2) To determine how yield responses to

defoliation can be explained by reductions in canopy light interception (through

reductions in leaf area below the critical leaf area index), 3) To determine how

response to defoliation at different reproductive stages differ, how responses

to sequential defoliation at two stages differ from defoliation at a single

stage, and how responses to simulated insect defoliation (through time) differ

from responses to defoliation on one day, 4) To characterize mechanisms of

soybean compensation to defoliation, particularly delayed leaf senescence and

altered leaf photosynthesis, 5) To compare responses at different sites (states)

and responses to determinate and indeterminate soybean growth habits (if the

study is conducted nationally), 6) To describe defoliation/yield loss

relationships for calculating single and multiple species EILs for defoliating

insect pests of soybean.

MATERIALS AND METHODS
The experimental design for soil treatments and for yield was a randomized

complete block containing four replications. Yield estimates each year were

determined for all 11 defoliation treatments. The effects of defoliation

treatments on soybean yield, light interception and photosynthesis were evaluated








by using ANOVA, subsequent treatment comparisons, and regression.

Treatments consist of 4 defoliation patterns (simulated insect defoliation

at stage R2, R4, R2R4, three levels within each pattern (defoliation to produce

a leaf area index of 3.5, 2.5, and 1.5 at late R4), and an undefoliated check.

Defoliation levels will be chosen based on the measured leaf area index (LAI) at

late Rl/early R2 and the projected LAI at R4 based, on the R4 measurement.

Defoliation levels will be chosen to provide final LAIs after defoliation (R4)

that include values above, at, and below the critical LAI (the LAI value at which

95% of incident light is intercepted by a plant canopy, estimated to be ca. 305

for soybean). Consequently, specific levels of defoliation may vary between

locations, but all locations will provide defoliation treatments that span the

critical LAI. Soybean will be defoliated by leaflet, and leaf areas of all

leaflets removed will be measured.

Defoliation will be limited to the upper two thirds of the canopy, but may

extend into the lower canopy if necessary for high defoliation levels. The

center 4 row-mm of the two middle rows will be defoliated. Undefoliated plots

will receive comparable handling (walking in plots and "fondling" plants) as

defoliated plots (to allow for compaction during defoliation and affects of

touching plants). Areas adjacent to the defoliated region (border rows and ends

of plots) will be sham defoliated (stripping leaflets without quantifying area

removed) to approximately the same level as the defoliated area. Border areas

should be sham defoliated during the last 8 days of the simulation (when most of

the defoliation is occurring), specifically on days 8 and 12. Treatments will

be color coded with stages or wood lath and flags to minimize potential errors

in leaf picking.

Target LAI's (3.5, 2.5, and 1.5) were chosen to provide discrete reductions

in light interception based a critical LAI of ca. 3.5. The undefoliated check









will provide a treatment above the critical LAI. Because the defoliation levels

depend on these target LAIs at late R4, an estimate of the R4 LAI is needed. The

LAI at R4 is likely to be 1-2 units greater than the LAI at late Rl/early R2

(when defoliation is initiated for certain treatments); therefore, the LAI at R4

will be approximately by the measured LAI at late Rl/early R2 + 2. Treatments

will be imposed by removing a given amount of leaf area (leaflets) based on the

difference in projected LAIs and the target LAIs. For the R2+4 sequential

defoliation treatments, the total leaf area to be removed will be calculated and

half removed at R2 and half removed at R4 (so that total leaf area removed is the

same as in the R2 and R4 treatments). The amount of leaf area removed is likely

to differ between states; however, the resulting treatments (LAI levels and

corresponding levels of light interception) will be comparable. For comparison,

the percent defoliation per treatment to achieve the target LAI can be calculated

from the formula:

[projected LAI target LAI)/projected LAI]*100

Defoliation will be imposed to approximate insect injury. Ideally, daily

injury rates and duration should be based on temperature driven consumption and

development models. However, because temperature driven models would result in

substantial differences in injury rates among locations, standard durations and

injury rates will be employed at all sites. Many soybean defoliators have larval

development times for the latter developmental stages (when >90% of consumption

occurs) of approximately two weeks, at temperatures commonly occurring in mid to

late summer. Consequently, defoliation will be imposed over 12 days (possibly,,

Monday of week 1 to Friday of week 2). Defoliation will occur at soybean stages

R2 and R4 (depending on treatment) which corresponds with the injury phenology

for many soybean defoliators.

Daily defoliation rates depend on stage specific consumption rates. In








brief, to simulate insect feeding we need to estimate what proportion of the

total defoliation required should occur on each day. The rationale behind the

values chosen is as follows. For this study, two aspects of development and

consumption are pertinent. First, proportion of total larval consumption in a

stage, and second, duration of developmental time in a stage. To determine the

proportion of the total defoliation that should occur in each larval stage, an

estimate of proportion of total consumption by stage is needed. Published data

on this question indicate that the proportion of total consumption by instars

are: GCW 1-2=2%, 3-4=8%, 5-6=90% (Hammond et al., 1979b); SBL 1-2=1%, 3-4=9%, 5-

6=90% (Boldt et al., 1975); and VBC 1-2=3%, 3-4=5%, 5-6=92% (Boldt et al., 1975).

Because so little defoliation occurs in the first two larval stages (<3%), for

this study we will consider defoliation only during the latter stages.

Specifically, we estimate the proportion of defoliation by stage as 3-4=10%, and

5-6=90%. The second question is duration of development time in a stage.

Literature data on green cloverworm (GCW), corn earworm (CEW), soybean looper

(SBL), and velvetbean caterpillar (VBC) were used to determine appropriate values

for this study. The proportion of time spent in various instars are: GCW 1-

2=29%, 3-4=26%, 5-6=45% (Hammond et al., 1975); and CEW 1-2=23%, 3-4=25%, 5-6=52%

(Boldt et al., (1975). Based on these values, an appropriate estimate of time

spent in each stage is 1-2=25%, 3-4=25%, and 5-6=5-%. We will estimate

development through stages 3-6 as requiring 12 days. Therefore, the ratio of

development times (25%:50% or 1:2) gives the number of days spent in each stage;

specifically, stages 3-4=4 days and stages 5-6=8 days. Consequently, to provide

an appropriate simulation of a lepidopteran defoliator of soybean (combining

consumption and development data), we will impose injury over 12 days with 2.5%

of the total defoliation occurring on each of the first 4 days and 11.25%

occurring on each of the last 8 days. Although designed to simulate lepidopteran








defoliation patterns, this simulation also is suitable for other species (such

as bean leaf beetle and grasshoppers). For adult defoliating insect (like

beetles) the increasing defoliation rates simulate an increasing pest population

rather than increasing consumption per individual pest.

All calculations of total leaf area to be removed, of % of this total leaf

area to be removed each day per plot, of the conversion of leaf area to be

removed to leaflets to be removed, and of defoliation summaries are provided by

a computer program, DEFOL (written by L. G. Higley for this project). To adjust

for possible discrepancies between projected and actual leaf area removed, all

leaf area removed/plot/day must be quantified and entered into the program to

allow for daily adjustments. The program will output leaf areas and numbers of

leaflets to be removed for each plot on each day. Leaf area to be removed is

based on target defoliation levels, appropriate injury rate, and previously

removed leaf area. Leaflets to be removed are calculated from leaf area to be

removed and a user-supplied estimate of average leaflet size on the first day.

Subsequently, the program calculates the average leaflet size based on number of

leaflets removed and measured leaf areas. Because the defoliation levels are

based on projected LAIs at R4, it is important to have an idea of actual LAIs

during defoliation so that adjustments can be made if the projections are greatly

in error. Measures of plant leaf area will be available from plant samples taken

immediately before the defoliation period, which provide a measure of the actual

LAIs. (To convert a mean plant leaf area into an LAI for 76 cm rows and 25

plants/row-m, multiply the plant leaf area (in cm2) by 0.00329). Records of

total leaf area actually removed (by plot) will be maintained to calculate actual

defoliation at end of the defoliation period.

Light interception will be measured in the plant canopy weekly from R3 to

R6 to include measures at each reproductive stage. A line quantum sensor, 76 cm








long, will be centered across the row and a measure of photosynthetically active

radiation (PAR) will be obtained. Measurement will be made for each of the two

center rows of each plot. Additionally, a measurement will be made outside the

plots, in full sun, for each block, to indicate PAR with 0% light interception.

All measurements will be taken within one hour of solar noon.
Individual.plant samples (for growth analysis) will be taken at ca. weekly

intervals at R2, R3, R4, and R6. For each sample date 3 plants/plot will be

removed, using a stratified random sampling procedure. Plants will be bagged,

labeled by treatment/block, and returned to the laboratory for measurements.

Immediately prior to defoliation at R2, plants will be randomly selected from any

of the 1.5 m areas on either end of the two center rows. On all other dates

plants will be selected randomly from any of the 1 m sections at the end the 4

m defoliated regions of the two center rows. Once a section is sampled

additional samples will not be taken from the same region, and the center 2 M of

the 2 center rows will not be sampled for growth analysis. The R3 sample will

be taken immediately after R2 defoliation and the R5 sample immediately after the

R4 defoliation. At harvest, individual plant samples (3 plants/plot) will be

taken from the center 2 m of the middle 2 rows. After individual plant samples

are obtained, all remaining plants in the defoliated, 4 m, middle two rows will

be harvested to provide plot yield. The appropriate sampling pattern is

indicated below (note that sections B and C are the defoliated areas):

Individual Plant Growth Samples R2 section A; R3, R4, R5, R6 section B1-B4

Individual Plant Yield Samples Section C; Plot Yield section B & C


-- A (1.5 m) --:-- BI (1 m) --:-- C (2 m) --:-- B2 (1 m) --:-- A (1.5 m) --

-- A (1.5 m) --:-- B3 (1 m) --:-- C (2 m) --:-- B4 (1 m) --:-- A 1.5 m ) --









Variable measured for growth analysis are: height (measure from

cotyledonary node), vegetative stage, reproductive stage, branches, nodes, lowest

leaf-bearing node (cotyledonary node=l, unifoliate node = 2, etc), leaves,

flowers, pods, leaf area, leaf dry weight, support (stem and petiole) dry weight,

and pod dry weight.

Variables measured for yield analysis (individual plant yield) are: 0

seeded pods, 1 seeded pods, 2 seeded pods, 3 seeded pods, 4 seeded pods, pod dry

weight (with seeds), seed dry weight, and support (stem) dry weight.

Variables measured for plot yield are yield and percent moisture.

Insecticide (Bacillus thuringiensis or other) will be applied as needed to

avoid confounding with natural insect injury. Fungicide benomyll or other) will

be applied at recommended rates at ca. RI to avoid confounding effects of

disease, if necessary. As much as possible only minimal pesticide application

will be made to avoid confounding effects.

Additional data to be maintained include agronomic practices herbicide

treatments, tillage, fungicides; soil factors soil type, soil pH, % organic

matter; weather data daily maximum and minimum temperatures, daily rainfall;

and important dates planting date, emergence date (80% emergence), sampling

dates for growth, light, individual yield, and plot yield. Other data may be

collected as necessary or indicated.

In specific states (Arkansas and Florida in the south) photosynthesis

measures will be taken weekly, from R1, to examine the soybean compensation to

defoliation through altered leaf photosynthesis. Leaflets at ca. nodes 6, 9, and

12 will be marked, and photosynthetic rates monitored before, during, and after

defoliation. Leaflets on at least two plants per plot will be measured.

Measurements will be made in full sunlight at comparable times for each

measurement.








Individual investigators are encouraged to conduct their own data analysis

as desired, however, data analysis by and across locations will be conducted at

Florida. For the national study data will be collated and analyzed at Nebraska.

Analysis will include calculation of variables for classical growth analysis and

for yield component analysis. Statistical procedures used will include analysis

of variance and regression techniques. All data were subjected to analysis of

variance. When the F test was significant, multiple range tests were applied.

RESULTS AND DISCUSSION

RAINFALL AND DROUGHT

Weather must be discussed in relation to defoliation results for 1990. The

summer of 1990 was one of the driest on record. The average rainfall for the

soybean growing of the previous 10 year period at Quincy, FL was 26 inches

compared to 12 inches for 1990. Soybean were definitely stressed during this

period, particularly from the R4 to R6 stage.

SEED, POD, AND STEM WEIGHT vs DEFOLIATION TREATMENT

Seed weight (Fig. 1) need to be reordered according to Alabama 1991 so that

R2R4-3.5, R4-3.5, R2-1.5, R4-2.5 are in descending order after R2-2.5. Alabama

seed weights were significantly different at the 0.0001 probability level for

1991, but were not significantly different in 1990. Arkansas seed weights were

not significantly different in 1990 or 1991.

NUMBER 0, 1, 2, 3 SEEDED PODS/PLANT VS DEFOLIATION TREATMENT

Number of pods with 0, 1, 2, 3 seeds per pods (Fig. 2 and 3) were not

significantly different in 1990 for Alabama or Arkansas. The 1991 data showed

no significant differences for Arkansas, but contained significant differences

for Alabama.

PHOTOSYNTHESIS vs. DEFOLIATION TREATMENT

Alabama did not participate in photosynthesis study. Arkansas data is





















a a a a
a a a a
l







I I I I 1 I i I I


I I I
ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
3.6 2.5 3.5 3.5 2.6 1.6 1.6 2.5 1.6


ab
b

............. ... ..- -/-- .... ... ----....
._b_ .

cda

. .. ... ......... ..
--d .. .



I i t i i I i i


I I I
ck ck R2R4 R4 R4 R21 R2 R2 R4 R2R4 R2R4
3.6 2.5 3.5 3.6 2.5 1.6 1.5 2.5 1.6


Defoliation Intensity


20

18 ------ -----








16 "" "' -------- --"---








0 I I I 1 I
4-

2-


ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
3.5 2.5 3.5 3.5 2.6 1.5 1.5 2.5 1.5


8 ---- ---- ----- -------------

6 .





2 ----


-a a a a .






0 I i I I I
4-




ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4R2R4
3.5 2.5 3.6 3.5 2.5 1.5 1.5 2.5 1.6


Defoliation Intensity






Fig. 1. Seed weight per soybean plant in relation

to yield for ranking defoliation

intensity (1990) of defoliation

treatments. Upper = AL and lower = AR;

left = 1990 and right = 1991.


q


an


--


n


- -- --


n










0
5 .. 1 Seeded Pod ..
S2 Seeded Pod
o --- 3 dd Pod
5 -- --- -"Total Pods-----

0-

5-
0 _. .. .. .......


6-

0---



0 1 1 1 I I I 1 I
ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
3.5 2.5 3.5 3.5 2.5 1.5 1.5 2.5 1.5


ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
3.5 2.5 3.5 3.5 2.5 1.5 1.5 2.5 1.5


Defoliation Intensity


--..... -18seded Pods--
-. 2 Seeded Pods
3 Seded Pods
-- ----- .-. -Total Pods---











S I- x -

I I I I l


I I I I I I
ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
3.5 2.5 3.5 3.5 2.5 1.5 1.5 2.5 1.5


70
I- Seeded Pods
60 -----:. 2 8--edd Pods
S3 Seeded Pods
Q 60------------- .- --------------- -~ ~ **;-.T-j(,| Pod ---~-
0
z 40 -- -Total Pod
',
40




It 20--



0

ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
3.5 2.5 3.5 3.5 2.5 1.5 1.5 2.5 1.5


Defoliation Intensity




Fig. 2. Mean number of pods with one, two and
three seeds in relation to defoliation
intensity and total pods per plant.
Upper = AL and lower = AR; left = 1990
and right = 1991.


SO


45

40

S35

, 30

S25

* 20

o
16

Z10

5


nI












e 4 ---- --- -
o
0



S----- -...............-- ....--..... .......





I I 0 I I 1 I1 I | 1
12R4 R2R4 ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
2.5 1.5 3.5 2.5 3.5 3.5 2.5 1.5 1.5 2.5 1.5

Defoliation Intensity


5








-2-
Z- ... .. .


I I I I 1 1 1 i 0 1 1 1 1 1 1 1 1 1 1 1
ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4 ck ck R2R4 R4 R4 R2 R2 R2 R4 R2R4 R2R4
3.6 2.5 3.5 3.5 2.5 1.6 1.5 2.5 1.5 3.5 2.5 3.5 3.5 2.5 1.5 1.5 2.5 1.5

Defoliation Intensity


Fig. 3. Mean number of pods with zero seeds in
relation to defoliation intensity. Upper
= AL and lower = AR; left = 1990 and
right = 1991.


0


'


I '


... _


... .. ... ...........






Soybean Physiological Stage


R2 R3 R4 R5 R6 R2 R3 R4 R5 R6
30

Defoliation Defoliation Defoliation Defoliation
.. . . .. 2 5

20

16



01



S ,



25 -
E
2 30







10-





0
220 226 230 235 240 245 250 255 260 265 270 276 280 285 215 220 225 230 235 240 246 250 255
JULIAN DAYS JULIAN DAYS



Fig. 4. Arkansas soybean photosynthesis (g/ mol m-1
s-1) in relation to defoliation period [R2
(top) and R4 (bottom)], year, and soybean
physiological stage. Defoliation level:
Control = *, 3.5 = +, 2.5 = *, 1.5 = 3.
Left = 1990 and right = 1991.









Soybean Physiological Stage

12 RS R4 R5 R6


0.8- -- --


0.6 ------. .- .--


0.4 --


0.2 ---------


0.8 ---- -6 -


0.6 ..----.-- -..- -


0.4 ------------.....-. -


0.2 ---


0.6 ---


0.4 -


0.2 ------


a


205 210 215


220 225 230 236 240 245 260 255 260 265 270
JULIAN DAYS


Fig. 5. Arkansas proportion of intercepted photo-
active-radiation (PIPAR) by soybean
canopy when defoliated at R2 (top), R2+4
(middle), and R4 (bottom) in relation to
Julian days and physiological stage of
growth. Defoliation level: Control = *,
3.5 = +, 2.5 = *, 1.5 = E. Left = 1990
and right = 1991.


''''''''''''


''''''''








presented in Fig. 4, but there were no significant differences between years or

defoliation treatments.

PROPORTION INTERCEPTED PHOTO-ACTIVE-RADIATION

Further information needed from Arkansas (Fig. 5).








ACKNOWLEDGEMENTS


Our thanks to E. Brown, Agric. Tech. IV; North Fla. Res. and Educ. Ctr.,

Univ. of Fla., Quincy, FL; for data collection, computer processing, and data

illustration.








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